Rw3G011220

Protein NRT1 PTR FAMILY 2.8-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003229
Physical Location & Seq
Reverse (-)
10885992 .. 10888149
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G011220.1

Sequence Viewer

Length: 1728 bp
ATGGAAGAAGGTTGGGGGAGAGGAGAGAACGCTAGGGAGCATGCGACAATGCATTCTTCATCCCCTCCCAAACAACCAGTCGGAGGGTGGAAAGCCGTCAGATACATCCTTGGGAATGAGACCTTTGAGAAATTGGCTTCCATGAGTTTGGTGGCAAATCTAGTGCTGTATCTGCATACCAAGTATAACTTGGATAATGTAGTTTCAGCTAATGTATACAATATCTGGTCTGGTTCATGCAATGTTGCACCCTTAATTGGTGCCTACCTTGCTGATACTCTTTTCGGAAAGTACTCTACTCTTCTTTATAGCTCAATAGCATCCCTCATGGGTATGGTGTCCTTGACATTAACTGCAAGTTTACATGAGCTAACTCCTCCTGCCTGCAATGGACAAGCGCAATGCGAACAACCCAATGCGTGGCAGAAGTTTGTCCTCTTTTCGGGTCTGGGACTGCTCGTTATTGGATCTGGAGGCCTCAGACCATGCAACATTGCCTTTGGAGCTGATCAATTCGACACCACAACAGAGAAGGGAAGAGCACAGCTAGATAGCTTTTGCAATTGGTGGTACTTTTTGTTCACCATTGCTCTCATTGTAGCTCTGACCGGAGTGGTCTACATACAAACTCATGTCAGCTGGGTTTTGGGTTTTGCTATACCAGCTGGATGCTTTGCCCTCTCCGTACTGATTTTCTTGCTCGGCCGAAAATTGTACATTCGTGTTAAGCCTCAAGGAAGCACCTTTGGGGACATAATGAGGGTAATTGTAGCTGCTTTTCGTAAGTTTAGAACAAAGACTTCAGGGCAGTCCCTTTATGATGCTCCTTCAATTGGACAAGAGTTGGAGCAAACACTTGCTCACACAGAGAGGTTCGAGTTTCTTGATAAGGCTGCTGTAATTGTAGATCCAAATGAATTGGATAGCAATGGCAACCCTAAAAGCGGTTGGAGACTATGCAGTGTGCAACAAGTTGAGCGACTCAAATGCGTCATAGCTTTGTTACCTGTTTGTATAAGTGGAATTGGTTGTTTCATAGGAATGCAGCAAATGCCATCATTTGGGACCTTTCAAGCTATTCAAACAAACAAAAAGATTGGAAACAATTTTGAAATCCCACCAGCCTGGATGTTTCTCACACAAATGATTGCACTTTCAATATGGATCATCATCTACGAATGCATTTACATCCCGTGGATTCAGAAAAGGAACAATGATGAATCTGGAAGATTGACAATGGAGACGAGATTCAAGGTTGGCATTGTGATGTCAATTCTGTGCATGGTGGTGGCTGGACTCACCGAAATGAAGCGCAGAAACTCTGCTTTGGAAAACAGATCTTTCGAGTCACCTATAACTGTTGCATTGCTTGTGCCACAATTTGCCTTGTCAGGTTTGATCGAGGCATTTGCAGCCATTGCCTTAATGGAGTTGCTTACAACACAGTGGCCAAAGAGCATGAGGACTTTTGCCGGGGCGGTCTTCTTTCTCAGCTTATCCATTGCAAGCTACTTCACCTCCATTCTTGTTAGCATTGTCAAGAAAGTGACTAGTTTGAATGGCAATACATCATGGCTGGGTGGTAATGACCTAAACAAGAACAGGCTTGATTACTACTATTACACCATTGCTGGTCTCGGGGTCTTGAACTTGTTGTATTTCCAATTCATTGCACGCCATTTTCTGTCCGATGCCGATGGTCATGACCGTTCTAGAATCCCCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04974 map04974
Pfam Domains
Protein Families
PTR2

Protein Analysis

575

Amino Acids

63.78

Weight (kDa)

8.7

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 98 - 526 3e-70 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 2 cut(s) 420, 1061
AccI GTMKAC 2 cut(s) 216, 618
AciI CCGC 2 cut(s) 945, 1478
AclWI GGATC 3 cut(s) 475, 902, 1172
AcoI YGGCCR 2 cut(s) 703, 1448
AcuI CTGAAG 1 cut(s) 786
AfaI GTAC 4 cut(s) 293, 572, 687, 716
AfiI CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
AgsI TTSAA 8 cut(s) 831, 1073, 1082, 1112, 1158, 1252, 1558, 1648
AhlI ACTAGT 1 cut(s) 1550
AjnI CCWGG 1 cut(s) 1124
Alw21I GWGCWC 1 cut(s) 544
Alw26I GTCTC 4 cut(s) 113, 946, 1235, 1640
AlwI GGATC 3 cut(s) 475, 902, 1172
Ama87I CYCGRG 1 cut(s) 1637
AoxI GGCC 3 cut(s) 475, 703, 1448
ApeKI GCWGC 4 cut(s) 773, 893, 1045, 1412
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
AspLEI GCGC 2 cut(s) 400, 1314
AspS9I GGNCC 1 cut(s) 1065
AsuC2I CCSGG 1 cut(s) 1474
AsuHPI GGTGA 4 cut(s) 574, 1291, 1341, 1507
AvaI CYCGRG 1 cut(s) 1637
AvaII GGWCC 1 cut(s) 1065
BalI TGGCCA 1 cut(s) 1450
BanI GGYRCC 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 1474
Bbv12I GWGCWC 1 cut(s) 544
BbvI GCAGC 4 cut(s) 760, 880, 1057, 1424
BccI CCATC 2 cut(s) 1063, 1691
BceAI ACGGC 1 cut(s) 80
BcgI CGANNNNNNTGC 4 cut(s) 969, 1003, 1391, 1425
BciT130I CCWGG 1 cut(s) 1126
BclI TGATCA 1 cut(s) 508
BcnI CCSGG 1 cut(s) 1474
BcoDI GTCTC 4 cut(s) 113, 946, 1235, 1640
BcuI ACTAGT 1 cut(s) 1550
BfaI CTAG 6 cut(s) 33, 161, 548, 1551, 1713, 1726
BglII AGATCT 1 cut(s) 1337
BisI GCNGC 4 cut(s) 774, 894, 1046, 1413
BlsI GCNGC 4 cut(s) 775, 895, 1047, 1414
BmcAI AGTACT 1 cut(s) 293
Bme1390I CCNGG 2 cut(s) 1126, 1474
Bme18I GGWCC 1 cut(s) 1065
BmeT110I CYCGRG 1 cut(s) 1637
BmgT120I GGNCC 1 cut(s) 1065
BmiI GGNNCC 2 cut(s) 262, 1066
BmrFI CCNGG 2 cut(s) 1126, 1474
BmsI GCATC 4 cut(s) 329, 659, 811, 1681
BpiI GAAGAC 1 cut(s) 1474
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 717
BpuMI CCSGG 1 cut(s) 1474
BsaBI GATNNNNATC 1 cut(s) 1169
BsaI GGTCTC 2 cut(s) 113, 1640
BsaJI CCNNGG 3 cut(s) 109, 1193, 1473
BsaWI WCCGGW 1 cut(s) 608
BsaXI ACNNNNNCTCC 2 cut(s) 1502, 1532
Bsc4I CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
Bse1I ACTGG 1 cut(s) 77
Bse8I GATNNNNATC 1 cut(s) 1169
BseBI CCWGG 1 cut(s) 1126
BseDI CCNNGG 3 cut(s) 109, 1193, 1473
BseGI GGATG 6 cut(s) 59, 105, 320, 674, 1134, 1188
BseJI GATNNNNATC 1 cut(s) 1169
BseLI CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
BseMII CTCAG 2 cut(s) 493, 1504
BseNI ACTGG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 36, 366
BseX3I CGGCCG 1 cut(s) 703
BseXI GCAGC 4 cut(s) 760, 880, 1057, 1424
BseYI CCCAGC 2 cut(s) 639, 1576
Bsh1285I CGRYCG 1 cut(s) 706
BshFI GGCC 3 cut(s) 477, 705, 1450
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 1637
BsiSI CCGG 2 cut(s) 609, 1473
BslFI GGGAC 4 cut(s) 465, 764, 796, 1078
BslI CCNNNNNNNGG 7 cut(s) 83, 257, 420, 442, 833, 944, 1061
BsmAI GTCTC 4 cut(s) 113, 946, 1235, 1640
BsmBI CGTCTC 1 cut(s) 1235
BsmFI GGGAC 4 cut(s) 465, 764, 796, 1078
BsmI GAATGC 3 cut(s) 52, 1047, 1184
BsnI GGCC 3 cut(s) 477, 705, 1450
Bso31I GGTCTC 2 cut(s) 113, 1640
BsoBI CYCGRG 1 cut(s) 1637
Bsp1286I GDGCHC 1 cut(s) 544
Bsp1407I TGTACA 1 cut(s) 714
Bsp143I GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
BspACI CCGC 2 cut(s) 945, 1478
BspANI GGCC 3 cut(s) 477, 705, 1450
BspCNI CTCAG 2 cut(s) 492, 1503
BspHI TCATGA 1 cut(s) 1702
BspLI GGNNCC 2 cut(s) 262, 1066
BspPI GGATC 3 cut(s) 475, 902, 1172
BspQI GCTCTTC 1 cut(s) 532
BspT107I GGYRCC 1 cut(s) 260
BspTNI GGTCTC 2 cut(s) 113, 1640
BsrGI TGTACA 1 cut(s) 714
BsrI ACTGG 1 cut(s) 77
BssECI CCNNGG 3 cut(s) 109, 1193, 1473
BssMI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
BssNAI GTATAC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 109
Bst1107I GTATAC 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 1126
Bst4CI ACNGT 3 cut(s) 1360, 1446, 1709
Bst6I CTCTTC 2 cut(s) 306, 532
BstAPI GCANNNNNTGC 2 cut(s) 1051, 1418
BstAUI TGTACA 1 cut(s) 714
BstC8I GCNNGC 4 cut(s) 42, 385, 1507, 1675
BstDEI CTNAG 2 cut(s) 479, 1490
BstDSI CCRYGG 1 cut(s) 1193
BstF5I GGATG 6 cut(s) 59, 105, 320, 674, 1134, 1188
BstHHI GCGC 2 cut(s) 400, 1314
BstKTI GATC 6 cut(s) 470, 511, 910, 1167, 1340, 1401
BstMAI GTCTC 4 cut(s) 113, 946, 1235, 1640
BstMBI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
BstMCI CGRYCG 1 cut(s) 706
BstMWI GCNNNNNNNGC 7 cut(s) 172, 269, 503, 662, 1051, 1412, 1418
BstNI CCWGG 1 cut(s) 1126
BstNSI RCATGY 1 cut(s) 44
BstSCI CCNGG 2 cut(s) 1124, 1472
BstV1I GCAGC 4 cut(s) 760, 880, 1057, 1424
BstV2I GAAGAC 1 cut(s) 1474
BstX2I RGATCY 3 cut(s) 467, 907, 1337
BstXI CCANNNNNNTGG 2 cut(s) 148, 1125
BstYI RGATCY 3 cut(s) 467, 907, 1337
BstZ17I GTATAC 1 cut(s) 217
BstZI CGGCCG 1 cut(s) 703
BsuRI GGCC 3 cut(s) 477, 705, 1450
BtgI CCRYGG 1 cut(s) 1193
BtsCI GGATG 6 cut(s) 59, 105, 320, 674, 1134, 1188
BtsI GCAGTG 1 cut(s) 967
BtsIMutI CAGTG 2 cut(s) 967, 1451
Cac8I GCNNGC 4 cut(s) 42, 385, 1507, 1675
CciI TCATGA 1 cut(s) 1702
CfoI GCGC 2 cut(s) 400, 1314
Cfr13I GGNCC 1 cut(s) 1065
CseI GACGC 1 cut(s) 979
Csp6I GTAC 4 cut(s) 292, 571, 686, 715
CviQI GTAC 4 cut(s) 292, 571, 686, 715
DdeI CTNAG 2 cut(s) 479, 1490
DpnI GATC 6 cut(s) 469, 510, 909, 1166, 1339, 1400
DpnII GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
EaeI YGGCCR 2 cut(s) 703, 1448
EagI CGGCCG 1 cut(s) 703
Eam1104I CTCTTC 2 cut(s) 306, 532
EarI CTCTTC 2 cut(s) 306, 532
EclXI CGGCCG 1 cut(s) 703
Eco130I CCWWGG 1 cut(s) 109
Eco147I AGGCCT 1 cut(s) 477
Eco31I GGTCTC 2 cut(s) 113, 1640
Eco47I GGWCC 1 cut(s) 1065
Eco52I CGGCCG 1 cut(s) 703
Eco57I CTGAAG 1 cut(s) 786
Eco88I CYCGRG 1 cut(s) 1637
EcoO109I RGGNCCY 1 cut(s) 1065
EcoRII CCWGG 1 cut(s) 1124
EcoT14I CCWWGG 1 cut(s) 109
EcoT22I ATGCAT 2 cut(s) 54, 1184
ErhI CCWWGG 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 1235
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 4 cut(s) 465, 764, 796, 1078
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 2 cut(s) 216, 618
Fnu4HI GCNGC 4 cut(s) 774, 894, 1046, 1413
FokI GGATG 6 cut(s) 46, 92, 307, 681, 1141, 1175
Fsp4HI GCNGC 4 cut(s) 774, 894, 1046, 1413
FspBI CTAG 6 cut(s) 33, 161, 548, 1551, 1713, 1726
GlaI GCGC 2 cut(s) 399, 1313
GluI GCNGC 4 cut(s) 774, 894, 1046, 1413
GsaI CCCAGC 2 cut(s) 643, 1580
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 3 cut(s) 477, 705, 1450
HapII CCGG 2 cut(s) 609, 1473
HgaI GACGC 1 cut(s) 979
HhaI GCGC 2 cut(s) 400, 1314
Hin6I GCGC 2 cut(s) 398, 1312
HinP1I GCGC 2 cut(s) 398, 1312
HinfI GANTC 7 cut(s) 981, 1198, 1220, 1248, 1296, 1346, 1716
HpaII CCGG 2 cut(s) 609, 1473
HphI GGTGA 4 cut(s) 574, 1291, 1341, 1507
Hpy166II GTNNAC 4 cut(s) 217, 362, 582, 619
Hpy188I TCNGA 7 cut(s) 83, 101, 287, 482, 606, 1203, 1690
Hpy188III TCNNGA 7 cut(s) 471, 884, 1224, 1540, 1645, 1703, 1713
Hpy8I GTNNAC 4 cut(s) 217, 362, 582, 619
HpyAV CCTTC 2 cut(s) 526, 837
HpyCH4III ACNGT 3 cut(s) 1360, 1446, 1709
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 269, 503, 662, 1051, 1412, 1418
HpyF3I CTNAG 2 cut(s) 479, 1490
HspAI GCGC 2 cut(s) 398, 1312
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
LguI GCTCTTC 1 cut(s) 532
LmnI GCTCC 4 cut(s) 37, 503, 829, 847
Lsp1109I GCAGC 4 cut(s) 760, 880, 1057, 1424
LweI GCATC 4 cut(s) 329, 659, 811, 1681
MaeI CTAG 6 cut(s) 33, 161, 548, 1551, 1713, 1726
MaeIII GTNAC 3 cut(s) 1002, 1347, 1546
MalI GATC 6 cut(s) 469, 510, 909, 1166, 1339, 1400
MboI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
MboII GAAGA 6 cut(s) 17, 48, 293, 549, 1239, 1474
MfeI CAATTG 2 cut(s) 562, 831
MflI RGATCY 3 cut(s) 467, 907, 1337
MhlI GDGCHC 1 cut(s) 544
MlsI TGGCCA 1 cut(s) 1450
MluNI TGGCCA 1 cut(s) 1450
MlyI GAGTC 3 cut(s) 975, 1290, 1355
MmeI TCCRAC 3 cut(s) 61, 825, 929
Mox20I TGGCCA 1 cut(s) 1450
Mph1103I ATGCAT 2 cut(s) 54, 1184
MscI TGGCCA 1 cut(s) 1450
MseI TTAA 4 cut(s) 254, 350, 726, 1424
MslI CAYNNNNRTG 6 cut(s) 332, 1040, 1142, 1265, 1286, 1304
Msp20I TGGCCA 1 cut(s) 1450
MspA1I CMGCKG 2 cut(s) 639, 665
MspI CCGG 2 cut(s) 609, 1473
MspR9I CCNGG 2 cut(s) 1126, 1474
MunI CAATTG 2 cut(s) 562, 831
Mva1269I GAATGC 3 cut(s) 52, 1047, 1184
MvaI CCWGG 1 cut(s) 1126
MwoI GCNNNNNNNGC 7 cut(s) 172, 269, 503, 662, 1051, 1412, 1418
NciI CCSGG 1 cut(s) 1474
NdeII GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
NlaIV GGNNCC 2 cut(s) 262, 1066
NmeAIII GCCGAG 1 cut(s) 681
NmuCI GTSAC 2 cut(s) 1347, 1546
NsiI ATGCAT 2 cut(s) 54, 1184
NspI RCATGY 1 cut(s) 44
PaeI GCATGC 1 cut(s) 44
PagI TCATGA 1 cut(s) 1702
PceI AGGCCT 1 cut(s) 477
PciSI GCTCTTC 1 cut(s) 532
PctI GAATGC 3 cut(s) 52, 1047, 1184
PfeI GAWTC 4 cut(s) 1198, 1220, 1248, 1716
PflMI CCANNNNNTGG 2 cut(s) 420, 1061
PkrI GCNGC 4 cut(s) 775, 895, 1047, 1414
PleI GAGTC 3 cut(s) 975, 1290, 1354
PpsI GAGTC 3 cut(s) 975, 1290, 1354
PpuMI RGGWCCY 1 cut(s) 1065
Psp5II RGGWCCY 1 cut(s) 1065
Psp6I CCWGG 1 cut(s) 1124
PspFI CCCAGC 2 cut(s) 639, 1576
PspGI CCWGG 1 cut(s) 1124
PspN4I GGNNCC 2 cut(s) 262, 1066
PspPI GGNCC 1 cut(s) 1065
PspPPI RGGWCCY 1 cut(s) 1065
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PsuI RGATCY 3 cut(s) 467, 907, 1337
PvuII CAGCTG 2 cut(s) 639, 665
RsaI GTAC 4 cut(s) 293, 572, 687, 716
RsaNI GTAC 4 cut(s) 292, 571, 686, 715
RseI CAYNNNNRTG 6 cut(s) 332, 1040, 1142, 1265, 1286, 1304
SapI GCTCTTC 1 cut(s) 532
SaqAI TTAA 4 cut(s) 254, 350, 726, 1424
SatI GCNGC 4 cut(s) 774, 894, 1046, 1413
Sau3AI GATC 6 cut(s) 467, 508, 907, 1164, 1337, 1398
Sau96I GGNCC 1 cut(s) 1065
ScaI AGTACT 1 cut(s) 293
SchI GAGTC 3 cut(s) 975, 1290, 1355
ScrFI CCNGG 2 cut(s) 1126, 1474
SduI GDGCHC 1 cut(s) 544
SfaNI GCATC 4 cut(s) 329, 659, 811, 1681
SinI GGWCC 1 cut(s) 1065
SmiMI CAYNNNNRTG 6 cut(s) 332, 1040, 1142, 1265, 1286, 1304
SmlI CTYRAG 1 cut(s) 732
SmoI CTYRAG 1 cut(s) 732
SpeI ACTAGT 1 cut(s) 1550
SphI GCATGC 1 cut(s) 44
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 2 cut(s) 945, 1478
SspMI CTAG 6 cut(s) 33, 161, 548, 1551, 1713, 1726
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 2 cut(s) 1124, 1472
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 3 cut(s) 1360, 1446, 1709
TaqI TCGA 4 cut(s) 516, 876, 1344, 1401
TatI WGTACW 2 cut(s) 291, 714
TfiI GAWTC 4 cut(s) 1198, 1220, 1248, 1716
Tru1I TTAA 4 cut(s) 254, 350, 726, 1424
Tru9I TTAA 4 cut(s) 254, 350, 726, 1424
TscAI CASTG 2 cut(s) 967, 1451
TseFI GTSAC 2 cut(s) 1347, 1546
TseI GCWGC 4 cut(s) 773, 893, 1045, 1412
Tsp45I GTSAC 2 cut(s) 1347, 1546
TspDTI ATGAA 7 cut(s) 48, 225, 930, 1024, 1233, 1322, 1657
TspGWI ACGGA 1 cut(s) 673
TspRI CASTG 2 cut(s) 967, 1451
Van91I CCANNNNNTGG 2 cut(s) 420, 1061
VpaK11BI GGWCC 1 cut(s) 1065
XbaI TCTAGA 1 cut(s) 1712
XceI RCATGY 1 cut(s) 44
XcmI CCANNNNNNNNNTGG 4 cut(s) 84, 148, 187, 1423
XmiI GTMKAC 2 cut(s) 216, 618
XspI CTAG 6 cut(s) 33, 161, 548, 1551, 1713, 1726
ZrmI AGTACT 1 cut(s) 293
Zsp2I ATGCAT 2 cut(s) 54, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.