FvH4_6g15740

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007950
Physical Location & Seq
Forward (+)
9821475 .. 9823913
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15740.t1

Sequence Viewer

Length: 1419 bp
ATGAAGCTCGTGAAACTCCAGATCCTTCATGGATCTCTAGCCCGGCGCGTGTTTTTCCGCGCTTTCTTTATCGCCGCGGCTATGTCTATTATTCCGTTGGTTCACATTTTATCCGGGTCTTACCTGAAAATGTTTAACTATGTGAGCACTATTGACTGTGCATCCGAATATGGCGGGGACAATGTCAACTTGGACCCGGGCAAGTTCATGTTTCAGACCCGGTTTCTGAATCCCATTTGGGGCGCTTTCGATGCCGAGAAGTGCAAGCAAGATGTGAACTTGACTGTAACGGTGGTCAGAGAGCTAATGGGCAAGCAGCTTTTGAATGGCGATAAGGCTCTCTGTGTTGGTGAAGGATCAGCCTCGGCCGTGATGGCTTTGCAAGATTTGGGGTTTCCTAATGCCCGTGGTGTTTATGAGCACAGATTCTTCTCCCTCAAGCACAAACAGTTTGTGTATGAGATTGACTATGAGGACAAGTCTTTTGACTTCGTGTTCTGTAGGGATCTAGACAAGGTTTCTGTCCCTGCTCTGCTTGTGCTCGAGATTGAGCGTGTTCTTAGCCCAGGTGGAATTGGGGCTATGCTTGTTGGTAGCAGTGGTTTGGCTCCGAATAGCCTGATACGGTCTGCTACCCCAATTTCTTCATTGTTGAAGAGTTCCAGTATCGTGCACGTTAATTATATCAGCAATTTCACACTGGTTGTGTTCAGGAAGAGTTACGAAAATGCTGGTCTATTTGAGCAGTATCGCCTTCCGGCGGACTGCCAATCCCTCACGAACAATAGACCTTTCATTGAGAAAATGGAGCCTCTTGTGATGGAGAAGTCTTTGGAGGATGGAGGAATGGTTACTTATCTGCCTAATGTTATTGATGTTTCCTCAAAAAAGCGGTTGGTCTATATCGATATTGGGGCAGCAGAGCATCTGAAATCAAATGTCACAAACTGGTTCCTGCCTTCTTATCCGATTACACACAAAGCTTTCCATGTTTATTTTGTTGACCACAACACTACTGTGCTATTGTCCTATGTCAAGAAGCCGGGAATCACCTTTGTTTATCATCCAGGGCTGGCTGGAATCAAGGACAAGAGCAGTGTTAACATTGAAGGAGAAGCAGATCCATATGTTGCAGATGAAGGATTTGATTTTCTTGATTGGTTCAAAGAAACAGTGCAGCATGCCGAGTTTGTGGTGCTTAAGATGAATGCAGGCAATGTTGAATTGAAGTTCCTGAGGGATCTATTTGAGAGTGGAGCCATATGCTTTGTCGACGAGCTCTTTCTTCACTGCTCTGGCAACATAGACGGGGAAGGTCCAATGCCAATAGATTGTATGGACATCTTCAAGAGCCTCCGAAGCAGTGGTGTGTATGTGCATCAGTGGTGGGGGGAAGACAACCCCGTCGAAGTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

473

Amino Acids

52.88

Weight (kDa)

5.66

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 378 - 463 1.4e-13 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1403
AccI GTMKAC 1 cut(s) 1272
AccII CGCG 3 cut(s) 48, 60, 77
AciI CCGC 6 cut(s) 58, 75, 77, 174, 761, 892
AclWI GGATC 6 cut(s) 16, 40, 364, 513, 1115, 1248
AcoI YGGCCR 1 cut(s) 366
AfiI CCNNNNNNNGG 2 cut(s) 239, 760
AflII CTTAAG 1 cut(s) 1199
AgsI TTSAA 7 cut(s) 325, 655, 1109, 1165, 1223, 1228, 1348
AjnI CCWGG 2 cut(s) 565, 1066
AleI CACNNNNGTG 1 cut(s) 1016
AluBI AGCT 5 cut(s) 7, 304, 319, 983, 1279
AluI AGCT 5 cut(s) 7, 304, 319, 983, 1279
Alw21I GWGCWC 5 cut(s) 149, 423, 543, 675, 1281
Alw44I GTGCAC 1 cut(s) 671
AlwI GGATC 6 cut(s) 16, 40, 364, 513, 1115, 1248
Ama87I CYCGRG 2 cut(s) 196, 542
AoxI GGCC 1 cut(s) 366
ApaLI GTGCAC 1 cut(s) 671
ApeKI GCWGC 3 cut(s) 316, 917, 1177
ArsI GACNNNNNNTTYG 2 cut(s) 467, 499
AspLEI GCGC 3 cut(s) 48, 62, 245
AspS9I GGNCC 2 cut(s) 193, 1316
AsuC2I CCSGG 6 cut(s) 43, 115, 197, 198, 220, 1044
AsuHPI GGTGA 2 cut(s) 362, 1042
AvaI CYCGRG 2 cut(s) 196, 542
AvaII GGWCC 2 cut(s) 193, 1316
AxyI CCTNAGG 1 cut(s) 1235
BaeGI GKGCMC 1 cut(s) 675
BanII GRGCYC 1 cut(s) 1281
BauI CACGAG 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 1401
Bbv12I GWGCWC 5 cut(s) 149, 423, 543, 675, 1281
BbvI GCAGC 3 cut(s) 328, 929, 1189
BccI CCATC 3 cut(s) 367, 814, 833
BceAI ACGGC 1 cut(s) 353
BciT130I CCWGG 2 cut(s) 567, 1068
BcnI CCSGG 6 cut(s) 43, 115, 197, 198, 220, 1044
BfaI CTAG 2 cut(s) 38, 509
BfmI CTRYAG 1 cut(s) 499
BfoI RGCGCY 1 cut(s) 246
BfrI CTTAAG 1 cut(s) 1199
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 5 cut(s) 75, 78, 317, 918, 1178
BlsI GCNGC 5 cut(s) 76, 79, 318, 919, 1179
Bme1390I CCNGG 8 cut(s) 43, 115, 197, 198, 220, 567, 1044, 1068
Bme18I GGWCC 2 cut(s) 193, 1316
BmeT110I CYCGRG 2 cut(s) 196, 542
BmgT120I GGNCC 2 cut(s) 193, 1316
BmiI GGNNCC 5 cut(s) 195, 609, 810, 953, 1258
BmrFI CCNGG 8 cut(s) 43, 115, 197, 198, 220, 567, 1044, 1068
BmsI GCATC 4 cut(s) 170, 241, 934, 1387
BpiI GAAGAC 1 cut(s) 1401
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 6 cut(s) 43, 115, 197, 198, 220, 1044
Bsa29I ATCGAT 1 cut(s) 906
BsaJI CCNNGG 6 cut(s) 75, 196, 363, 406, 565, 1067
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 760
Bse1I ACTGG 3 cut(s) 663, 705, 953
Bse21I CCTNAGG 1 cut(s) 1235
Bse3DI GCAATG 1 cut(s) 1222
BseBI CCWGG 2 cut(s) 567, 1068
BseCI ATCGAT 1 cut(s) 906
BseDI CCNNGG 6 cut(s) 75, 196, 363, 406, 565, 1067
BseGI GGATG 3 cut(s) 161, 844, 1063
BseLI CCNNNNNNNGG 2 cut(s) 239, 760
BseMI GCAATG 1 cut(s) 1222
BseMII CTCAG 1 cut(s) 1226
BseNI ACTGG 3 cut(s) 663, 705, 953
BseSI GKGCMC 1 cut(s) 675
BseX3I CGGCCG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 328, 929, 1189
BsgI GTGCAG 1 cut(s) 1196
Bsh1236I CGCG 3 cut(s) 48, 60, 77
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 368
BshVI ATCGAT 1 cut(s) 906
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 5 cut(s) 149, 423, 543, 675, 1281
BsiHKCI CYCGRG 2 cut(s) 196, 542
BsiSI CCGG 6 cut(s) 43, 114, 197, 220, 758, 1043
BslFI GGGAC 2 cut(s) 191, 509
BslI CCNNNNNNNGG 2 cut(s) 239, 760
BsmFI GGGAC 2 cut(s) 191, 509
BsmI GAATGC 1 cut(s) 1213
BsnI GGCC 1 cut(s) 368
BsoBI CYCGRG 2 cut(s) 196, 542
Bsp1286I GDGCHC 5 cut(s) 149, 423, 543, 675, 1281
Bsp143I GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
BspACI CCGC 6 cut(s) 58, 75, 77, 174, 761, 892
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 1 cut(s) 1227
BspDI ATCGAT 1 cut(s) 906
BspFNI CGCG 3 cut(s) 48, 60, 77
BspLI GGNNCC 5 cut(s) 195, 609, 810, 953, 1258
BspPI GGATC 6 cut(s) 16, 40, 364, 513, 1115, 1248
BspTI CTTAAG 1 cut(s) 1199
BsrDI GCAATG 1 cut(s) 1222
BsrI ACTGG 3 cut(s) 663, 705, 953
BssECI CCNNGG 6 cut(s) 75, 196, 363, 406, 565, 1067
BssMI GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 2 cut(s) 567, 1068
Bst4CI ACNGT 7 cut(s) 158, 286, 292, 450, 627, 1018, 1174
Bst6I CTCTTC 2 cut(s) 650, 710
BstAFI CTTAAG 1 cut(s) 1199
BstC8I GCNNGC 5 cut(s) 266, 314, 1074, 1182, 1213
BstDEI CTNAG 2 cut(s) 560, 1235
BstDSI CCRYGG 2 cut(s) 75, 406
BstF5I GGATG 3 cut(s) 161, 844, 1063
BstFNI CGCG 3 cut(s) 48, 60, 77
BstH2I RGCGCY 1 cut(s) 246
BstHHI GCGC 3 cut(s) 48, 62, 245
BstKTI GATC 6 cut(s) 24, 35, 359, 508, 1123, 1243
BstMBI GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 3 cut(s) 251, 374, 1359
BstNI CCWGG 2 cut(s) 567, 1068
BstNSI RCATGY 1 cut(s) 1184
BstSCI CCNGG 8 cut(s) 41, 113, 195, 196, 218, 565, 1042, 1066
BstSFI CTRYAG 1 cut(s) 499
BstSLI GKGCMC 1 cut(s) 675
BstUI CGCG 3 cut(s) 48, 60, 77
BstV1I GCAGC 3 cut(s) 328, 929, 1189
BstV2I GAAGAC 1 cut(s) 1401
BstX2I RGATCY 5 cut(s) 21, 32, 505, 1120, 1240
BstYI RGATCY 5 cut(s) 21, 32, 505, 1120, 1240
BstZI CGGCCG 1 cut(s) 366
Bsu15I ATCGAT 1 cut(s) 906
Bsu36I CCTNAGG 1 cut(s) 1235
BsuRI GGCC 1 cut(s) 368
BsuTUI ATCGAT 1 cut(s) 906
BtgI CCRYGG 2 cut(s) 75, 406
BtsCI GGATG 3 cut(s) 161, 844, 1063
BtsI GCAGTG 4 cut(s) 604, 1102, 1288, 1369
BtsIMutI CAGTG 7 cut(s) 604, 698, 1102, 1179, 1288, 1369, 1388
Cac8I GCNNGC 5 cut(s) 266, 314, 1074, 1182, 1213
CfoI GCGC 3 cut(s) 48, 62, 245
Cfr13I GGNCC 2 cut(s) 193, 1316
Cfr42I CCGCGG 1 cut(s) 78
Cfr9I CCCGGG 1 cut(s) 196
ClaI ATCGAT 1 cut(s) 906
CviAII CATG 4 cut(s) 29, 208, 989, 1181
DdeI CTNAG 2 cut(s) 560, 1235
DpnI GATC 6 cut(s) 23, 34, 358, 507, 1122, 1242
DpnII GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
DrdI GACNNNNNNGTC 1 cut(s) 1403
DseDI GACNNNNNNGTC 1 cut(s) 1403
EaeI YGGCCR 1 cut(s) 366
EagI CGGCCG 1 cut(s) 366
Eam1104I CTCTTC 2 cut(s) 650, 710
EarI CTCTTC 2 cut(s) 650, 710
EciI GGCGGA 1 cut(s) 776
Ecl136II GAGCTC 1 cut(s) 1279
EclXI CGGCCG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 1281
Eco47I GGWCC 2 cut(s) 193, 1316
Eco52I CGGCCG 1 cut(s) 366
Eco53kI GAGCTC 1 cut(s) 1279
Eco81I CCTNAGG 1 cut(s) 1235
Eco88I CYCGRG 2 cut(s) 196, 542
EcoICRI GAGCTC 1 cut(s) 1279
EcoRII CCWGG 2 cut(s) 565, 1066
EcoT38I GRGCYC 1 cut(s) 1281
FaeI CATG 4 cut(s) 32, 211, 992, 1184
FaqI GGGAC 2 cut(s) 191, 509
FatI CATG 4 cut(s) 28, 207, 988, 1180
FauI CCCGC 1 cut(s) 167
FauNDI CATATG 2 cut(s) 1126, 1262
FblI GTMKAC 1 cut(s) 1272
Fnu4HI GCNGC 5 cut(s) 75, 78, 317, 918, 1178
FokI GGATG 3 cut(s) 148, 851, 1050
FriOI GRGCYC 1 cut(s) 1281
Fsp4HI GCNGC 5 cut(s) 75, 78, 317, 918, 1178
FspBI CTAG 2 cut(s) 38, 509
GlaI GCGC 3 cut(s) 47, 61, 244
GluI GCNGC 5 cut(s) 75, 78, 317, 918, 1178
HaeII RGCGCY 1 cut(s) 246
HaeIII GGCC 1 cut(s) 368
HapII CCGG 6 cut(s) 43, 114, 197, 220, 758, 1043
HhaI GCGC 3 cut(s) 48, 62, 245
Hin1II CATG 4 cut(s) 32, 211, 992, 1184
Hin6I GCGC 3 cut(s) 46, 60, 243
HinP1I GCGC 3 cut(s) 46, 60, 243
HincII GTYRAC 4 cut(s) 187, 1003, 1102, 1273
HindII GTYRAC 4 cut(s) 187, 1003, 1102, 1273
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 4 cut(s) 229, 426, 1047, 1080
HpaI GTTAAC 1 cut(s) 1102
HpaII CCGG 6 cut(s) 43, 114, 197, 220, 758, 1043
HphI GGTGA 2 cut(s) 362, 1042
Hpy166II GTNNAC 7 cut(s) 103, 187, 277, 673, 1003, 1102, 1273
Hpy188I TCNGA 8 cut(s) 166, 216, 228, 299, 612, 930, 969, 1358
Hpy8I GTNNAC 7 cut(s) 103, 187, 277, 673, 1003, 1102, 1273
Hpy99I CGWCG 2 cut(s) 1277, 1409
HpyAV CCTTC 7 cut(s) 35, 347, 764, 969, 1103, 1133, 1307
HpyCH4III ACNGT 7 cut(s) 158, 286, 292, 450, 627, 1018, 1174
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 8 cut(s) 161, 264, 382, 673, 1133, 1177, 1211, 1378
HpyF10VI GCNNNNNNNGC 3 cut(s) 251, 374, 1359
HpyF3I CTNAG 2 cut(s) 560, 1235
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 4 cut(s) 32, 211, 992, 1184
HspAI GCGC 3 cut(s) 46, 60, 243
KspAI GTTAAC 1 cut(s) 1102
KspI CCGCGG 1 cut(s) 78
Kzo9I GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
LmnI GCTCC 3 cut(s) 613, 808, 1256
Lsp1109I GCAGC 3 cut(s) 328, 929, 1189
LweI GCATC 4 cut(s) 170, 241, 934, 1387
MaeI CTAG 2 cut(s) 38, 509
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 4 cut(s) 286, 719, 850, 940
MalI GATC 6 cut(s) 23, 34, 358, 507, 1122, 1242
MboI GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
MboII GAAGA 7 cut(s) 421, 636, 667, 727, 1277, 1336, 1406
MflI RGATCY 5 cut(s) 21, 32, 505, 1120, 1240
MhlI GDGCHC 5 cut(s) 149, 423, 543, 675, 1281
MluCI AATT 5 cut(s) 573, 639, 679, 691, 1223
MseI TTAA 4 cut(s) 135, 678, 1101, 1200
MslI CAYNNNNRTG 1 cut(s) 1016
MspA1I CMGCKG 1 cut(s) 77
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 6 cut(s) 43, 114, 197, 220, 758, 1043
MspR9I CCNGG 8 cut(s) 43, 115, 197, 198, 220, 567, 1044, 1068
Mva1269I GAATGC 1 cut(s) 1213
MvaI CCWGG 2 cut(s) 567, 1068
MvnI CGCG 3 cut(s) 48, 60, 77
MwoI GCNNNNNNNGC 3 cut(s) 251, 374, 1359
NciI CCSGG 6 cut(s) 43, 115, 197, 198, 220, 1044
NdeI CATATG 2 cut(s) 1126, 1262
NdeII GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
NlaIII CATG 4 cut(s) 32, 211, 992, 1184
NlaIV GGNNCC 5 cut(s) 195, 609, 810, 953, 1258
NmeAIII GCCGAG 3 cut(s) 280, 344, 1210
NmuCI GTSAC 1 cut(s) 940
NspI RCATGY 1 cut(s) 1184
OliI CACNNNNGTG 1 cut(s) 1016
PaeI GCATGC 1 cut(s) 1184
PaeR7I CTCGAG 1 cut(s) 542
PctI GAATGC 1 cut(s) 1213
PfeI GAWTC 4 cut(s) 229, 426, 1047, 1080
PflFI GACNNNGTC 1 cut(s) 182
PkrI GCNGC 5 cut(s) 76, 79, 318, 919, 1179
Psp124BI GAGCTC 1 cut(s) 1281
Psp6I CCWGG 2 cut(s) 565, 1066
PspGI CCWGG 2 cut(s) 565, 1066
PspN4I GGNNCC 5 cut(s) 195, 609, 810, 953, 1258
PspPI GGNCC 2 cut(s) 193, 1316
PsuI RGATCY 5 cut(s) 21, 32, 505, 1120, 1240
PsyI GACNNNGTC 1 cut(s) 182
RseI CAYNNNNRTG 1 cut(s) 1016
SacI GAGCTC 1 cut(s) 1281
SacII CCGCGG 1 cut(s) 78
SalI GTCGAC 1 cut(s) 1271
SaqAI TTAA 4 cut(s) 135, 678, 1101, 1200
SatI GCNGC 5 cut(s) 75, 78, 317, 918, 1178
Sau3AI GATC 6 cut(s) 21, 32, 356, 505, 1120, 1240
Sau96I GGNCC 2 cut(s) 193, 1316
ScrFI CCNGG 8 cut(s) 43, 115, 197, 198, 220, 567, 1044, 1068
SduI GDGCHC 5 cut(s) 149, 423, 543, 675, 1281
SfaNI GCATC 4 cut(s) 170, 241, 934, 1387
SfcI CTRYAG 1 cut(s) 499
Sfr274I CTCGAG 1 cut(s) 542
Sfr303I CCGCGG 1 cut(s) 78
SgrBI CCGCGG 1 cut(s) 78
SinI GGWCC 2 cut(s) 193, 1316
SlaI CTCGAG 1 cut(s) 542
SmaI CCCGGG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 1016
SmlI CTYRAG 3 cut(s) 437, 542, 1199
SmoI CTYRAG 3 cut(s) 437, 542, 1199
SphI GCATGC 1 cut(s) 1184
Sse9I AATT 5 cut(s) 573, 639, 679, 691, 1223
SsiI CCGC 6 cut(s) 58, 75, 77, 174, 761, 892
SspMI CTAG 2 cut(s) 38, 509
SstI GAGCTC 1 cut(s) 1281
StyD4I CCNGG 8 cut(s) 41, 113, 195, 196, 218, 565, 1042, 1066
TaaI ACNGT 7 cut(s) 158, 286, 292, 450, 627, 1018, 1174
TaiI ACGT 1 cut(s) 678
TaqI TCGA 5 cut(s) 249, 543, 906, 1272, 1407
TasI AATT 5 cut(s) 573, 639, 679, 691, 1223
TauI GCSGC 2 cut(s) 77, 80
TfiI GAWTC 4 cut(s) 229, 426, 1047, 1080
Tru1I TTAA 4 cut(s) 135, 678, 1101, 1200
Tru9I TTAA 4 cut(s) 135, 678, 1101, 1200
TscAI CASTG 7 cut(s) 604, 705, 1102, 1179, 1295, 1369, 1388
TseFI GTSAC 1 cut(s) 940
TseI GCWGC 3 cut(s) 316, 917, 1177
Tsp45I GTSAC 1 cut(s) 940
TspDTI ATGAA 7 cut(s) 17, 17, 196, 636, 784, 1152, 1220
TspGWI ACGGA 1 cut(s) 84
TspMI CCCGGG 1 cut(s) 196
TspRI CASTG 7 cut(s) 604, 705, 1102, 1179, 1295, 1369, 1388
Tth111I GACNNNGTC 1 cut(s) 182
Vha464I CTTAAG 1 cut(s) 1199
VneI GTGCAC 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 193, 1316
XbaI TCTAGA 1 cut(s) 508
XceI RCATGY 1 cut(s) 1184
XcmI CCANNNNNNNNNTGG 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 542
XmaI CCCGGG 1 cut(s) 196
XmiI GTMKAC 1 cut(s) 1272
XspI CTAG 2 cut(s) 38, 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.