pycom04g09410

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0007950
Physical Location & Seq
Reverse (-)
12016390 .. 12017868
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g09410.1

Sequence Viewer

Length: 1479 bp
ATGATTCAGGCCCGAATCGTGGAAACGAAGAAAATCATGGATTTGAAGGGCGTGAGAATGCAGATCCTTCATGGGTCTATGGCCCGGCGCGTGTTTCTCTGGGCATTCTTGATCGCCGCGGCTATGTCGATCATACGATCGATCCACATTTTGTCCGGGACCTACATGACAATGTTCACTTTTGTGAACTCTGGCGACTGCGCCGCCGAACTGGGCCGCGTTGTCCCTGTCGAATTGACCACGGAGAAGTTCAATTTCCAGAGTCGGTTCCTGAACCCCTTCTGGGGGTCTTACGATTCGGAGCAGTGCGTGCGCGATGTGAATTTGACTGTCAGTGTGGTCAGAGAGCTCATGGGTCAGAAGCTATTGAAATACGGTACAAAAGCTCTCTCTGTAGGAGAGGATTCCGCCTCAGCCGTGCTGGCATTGCAAGCTTTGGGATTTCCCAATGCTCGTGGTGTGTACAAACACCGGTTCTTCTCGCTCAAGCACAAACAGTTTGTGTACGAAATTGACTATGTGGACAAGTCTTTCGATTTTGTGCTTTCCAGGGATCTCGATAAGGTTTCGGTCCCTGCGCTACTAGTGCTTGAGATCGAGCGTGTTCTTAGCCCTGGTGGAATTGGGGCTATGCTTGTGGGTAGCAGTGTTTCCTCCCCGAATAGCTTGATTAGGTCTGCTACTCCAATTTCTTCACTGCTGAAAAGTTCCAGTGTTGTGTACGTTAATTACGTCAATAACTTCACACTGGTTGTGTTCAAGAAAAATTATGACAATGCTGGTCTCTTTGAGCAGTATCGCCTTCCAGCTGACTGCCGATCCCTCACAAACAATCGACCTCTCATTGGGAAAATGGAGCCTCTGGTGAAGGAAAAACCAGTGGAGTATGGAAGGAGGTTCGTCTATTTGCCTAATTTTGTTGATCTTTCCTCAAAGAGGCGTTTGGTCTATATTGATATTGGGGCAGCACAGCATCTGAACTCAAATGTTACAAATTGGTTCCTGCCTTCTTATCCCATCGAACACAAAGATTTCAATGTTTATTTTGTTGACCATAACACTTCTGTTCTATTATCCTATGTCAAAAAGCCGGGAATTACCTTTGTTTATCATCCAGGGCTGGCCGGAATCAAGCCAAAAGTCAATGTTACAATCGATGGAGACACAGATCCATATGTCGGAGATGAAGGGTTTGATTTCCTCGTTTGGTTCAAAGAAACAGTGGGGTTTGCAGATTTCGTGGTGCTTAAGATGAATGCAGGCAGTGTAGAACTGAAGTTCCTCACGGAACTGTTTGAAAGTGGAGCCATATGCTTTGTGGACGAGCTGTTTCTTCACTGCTCAGGCCAAGTAGATGCTGGAGGTGCAATGCCGGTGGACTGCATGGACATCTTCGGAAGCCTCCGCAGCAGTGGTGTGTTTGTCCATCAGTGGTGGGGAGATGGCAACCCCGGTGATGTTCTCCTATCTGTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

493

Amino Acids

54.9

Weight (kDa)

7.14

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 291 - 354 3.5e-06 Domain of unknown function (DUF7870)
DUF7870 PF25276 401 - 479 1e-13 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 90, 119, 219, 315
AciI CCGC 6 cut(s) 117, 119, 204, 217, 408, 1405
AclWI GGATC 5 cut(s) 58, 136, 561, 813, 1163
AcoI YGGCCR 1 cut(s) 1122
AcsI RAATTY 1 cut(s) 322
AcuI CTGAAG 1 cut(s) 1295
AfaI GTAC 4 cut(s) 379, 464, 506, 722
AfiI CCNNNNNNNGG 7 cut(s) 19, 283, 284, 285, 845, 936, 1178
AflII CTTAAG 1 cut(s) 1247
AgeI ACCGGT 1 cut(s) 471
AgsI TTSAA 7 cut(s) 46, 253, 370, 760, 1036, 1213, 1298
AhlI ACTAGT 1 cut(s) 583
AjnI CCWGG 3 cut(s) 548, 613, 1114
AleI CACNNNNGTG 1 cut(s) 182
AloI GAACNNNNNNTCC 2 cut(s) 1263, 1295
AluBI AGCT 7 cut(s) 349, 364, 386, 434, 666, 809, 1327
AluI AGCT 7 cut(s) 349, 364, 386, 434, 666, 809, 1327
Alw21I GWGCWC 1 cut(s) 351
Alw26I GTCTC 2 cut(s) 788, 1155
AlwI GGATC 5 cut(s) 58, 136, 561, 813, 1163
AlwNI CAGNNNCTG 1 cut(s) 976
AoxI GGCC 5 cut(s) 9, 81, 214, 1122, 1345
ApeKI GCWGC 2 cut(s) 965, 1407
ApoI RAATTY 1 cut(s) 322
ArsI GACNNNNNNTTYG 2 cut(s) 515, 547
AsiGI ACCGGT 1 cut(s) 471
Asp700I GAANNNNTTC 1 cut(s) 278
AspLEI GCGC 4 cut(s) 90, 203, 315, 580
AspS9I GGNCC 5 cut(s) 10, 82, 159, 214, 571
AsuC2I CCSGG 4 cut(s) 85, 157, 1092, 1452
AsuHPI GGTGA 2 cut(s) 877, 1466
AvaII GGWCC 2 cut(s) 159, 571
BanII GRGCYC 1 cut(s) 351
BauI CACGAG 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 412
BbvI GCAGC 2 cut(s) 977, 1419
BccI CCATC 4 cut(s) 1025, 1151, 1434, 1436
BceAI ACGGC 1 cut(s) 401
BciT130I CCWGG 3 cut(s) 550, 615, 1116
BcnI CCSGG 4 cut(s) 85, 157, 1092, 1452
BcoDI GTCTC 2 cut(s) 788, 1155
BcuI ACTAGT 1 cut(s) 583
BfaI CTAG 1 cut(s) 584
BfmI CTRYAG 1 cut(s) 393
BfrI CTTAAG 1 cut(s) 1247
BglI GCCNNNNNGGC 1 cut(s) 422
BisI GCNGC 6 cut(s) 117, 120, 204, 217, 966, 1408
BlsI GCNGC 6 cut(s) 118, 121, 205, 218, 967, 1409
Bme1390I CCNGG 7 cut(s) 85, 157, 550, 615, 1092, 1116, 1452
Bme18I GGWCC 2 cut(s) 159, 571
BmgT120I GGNCC 5 cut(s) 10, 82, 159, 214, 571
BmiI GGNNCC 6 cut(s) 160, 269, 573, 858, 1001, 1306
BmrFI CCNGG 7 cut(s) 85, 157, 550, 615, 1092, 1116, 1452
BmrI ACTGGG 1 cut(s) 221
BmsI GCATC 2 cut(s) 982, 1345
BmuI ACTGGG 1 cut(s) 221
BpmI CTGGAG 1 cut(s) 1380
Bpu10I CCTNAGC 2 cut(s) 412, 1342
BpuEI CTTGAG 2 cut(s) 470, 611
BpuMI CCSGG 4 cut(s) 85, 157, 1092, 1452
Bsa29I ATCGAT 2 cut(s) 140, 1155
BsaI GGTCTC 1 cut(s) 788
BsaJI CCNNGG 6 cut(s) 117, 240, 549, 613, 1115, 1450
BsaWI WCCGGW 1 cut(s) 471
Bsc4I CCNNNNNNNGG 7 cut(s) 19, 283, 284, 285, 845, 936, 1178
Bse118I RCCGGY 2 cut(s) 471, 1372
Bse1I ACTGG 4 cut(s) 216, 711, 753, 878
Bse3DI GCAATG 2 cut(s) 425, 1374
BseBI CCWGG 3 cut(s) 550, 615, 1116
BseCI ATCGAT 2 cut(s) 140, 1155
BseDI CCNNGG 6 cut(s) 117, 240, 549, 613, 1115, 1450
BseGI GGATG 1 cut(s) 1111
BseLI CCNNNNNNNGG 7 cut(s) 19, 283, 284, 285, 845, 936, 1178
BseMI GCAATG 2 cut(s) 425, 1374
BseMII CTCAG 2 cut(s) 426, 1356
BseNI ACTGG 4 cut(s) 216, 711, 753, 878
BseXI GCAGC 2 cut(s) 977, 1419
Bsh1236I CGCG 4 cut(s) 90, 119, 219, 315
Bsh1285I CGRYCG 1 cut(s) 140
BshFI GGCC 5 cut(s) 11, 83, 216, 1124, 1347
BshTI ACCGGT 1 cut(s) 471
BshVI ATCGAT 2 cut(s) 140, 1155
BsiEI CGRYCG 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 351
BsiSI CCGG 7 cut(s) 85, 156, 472, 1091, 1125, 1373, 1452
BslFI GGGAC 3 cut(s) 172, 209, 557
BslI CCNNNNNNNGG 7 cut(s) 19, 283, 284, 285, 845, 936, 1178
BsmAI GTCTC 2 cut(s) 788, 1155
BsmFI GGGAC 3 cut(s) 172, 209, 557
BsmI GAATGC 3 cut(s) 63, 104, 1261
BsnI GGCC 5 cut(s) 11, 83, 216, 1124, 1347
Bso31I GGTCTC 1 cut(s) 788
Bsp1286I GDGCHC 1 cut(s) 351
Bsp1407I TGTACA 1 cut(s) 462
BspACI CCGC 6 cut(s) 117, 119, 204, 217, 408, 1405
BspANI GGCC 5 cut(s) 11, 83, 216, 1124, 1347
BspCNI CTCAG 2 cut(s) 425, 1355
BspDI ATCGAT 2 cut(s) 140, 1155
BspFNI CGCG 4 cut(s) 90, 119, 219, 315
BspLI GGNNCC 6 cut(s) 160, 269, 573, 858, 1001, 1306
BspPI GGATC 5 cut(s) 58, 136, 561, 813, 1163
BspTI CTTAAG 1 cut(s) 1247
BspTNI GGTCTC 1 cut(s) 788
BsrDI GCAATG 2 cut(s) 425, 1374
BsrFI RCCGGY 2 cut(s) 471, 1372
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 4 cut(s) 216, 711, 753, 878
BssAI RCCGGY 2 cut(s) 471, 1372
BssECI CCNNGG 6 cut(s) 117, 240, 549, 613, 1115, 1450
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 550, 615, 1116
Bst4CI ACNGT 5 cut(s) 331, 377, 498, 1222, 1293
BstAFI CTTAAG 1 cut(s) 1247
BstAPI GCANNNNNTGC 1 cut(s) 310
BstAUI TGTACA 1 cut(s) 462
BstC8I GCNNGC 5 cut(s) 311, 423, 432, 1122, 1261
BstDEI CTNAG 3 cut(s) 412, 608, 1342
BstDSI CCRYGG 2 cut(s) 117, 240
BstENI CCTNNNNNAGG 1 cut(s) 934
BstF5I GGATG 1 cut(s) 1111
BstFNI CGCG 4 cut(s) 90, 119, 219, 315
BstHHI GCGC 4 cut(s) 90, 203, 315, 580
BstMAI GTCTC 2 cut(s) 788, 1155
BstMCI CGRYCG 1 cut(s) 140
BstMWI GCNNNNNNNGC 7 cut(s) 310, 422, 427, 431, 586, 1364, 1407
BstNI CCWGG 3 cut(s) 550, 615, 1116
BstSCI CCNGG 7 cut(s) 83, 155, 548, 613, 1090, 1114, 1450
BstSFI CTRYAG 1 cut(s) 393
BstUI CGCG 4 cut(s) 90, 119, 219, 315
BstV1I GCAGC 2 cut(s) 977, 1419
BstX2I RGATCY 3 cut(s) 63, 553, 1168
BstYI RGATCY 3 cut(s) 63, 553, 1168
Bsu15I ATCGAT 2 cut(s) 140, 1155
BsuRI GGCC 5 cut(s) 11, 83, 216, 1124, 1347
BsuTUI ATCGAT 2 cut(s) 140, 1155
BtgI CCRYGG 2 cut(s) 117, 240
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 1111
BtsI GCAGTG 6 cut(s) 311, 652, 695, 1270, 1336, 1417
Cac8I GCNNGC 5 cut(s) 311, 423, 432, 1122, 1261
CaiI CAGNNNCTG 1 cut(s) 976
CfoI GCGC 4 cut(s) 90, 203, 315, 580
Cfr10I RCCGGY 2 cut(s) 471, 1372
Cfr13I GGNCC 5 cut(s) 10, 82, 159, 214, 571
Cfr42I CCGCGG 1 cut(s) 120
ClaI ATCGAT 2 cut(s) 140, 1155
Csp6I GTAC 4 cut(s) 378, 463, 505, 721
CspAI ACCGGT 1 cut(s) 471
CspCI CAANNNNNGTGG 4 cut(s) 436, 471, 1356, 1391
CviAII CATG 5 cut(s) 37, 71, 166, 352, 1384
CviQI GTAC 4 cut(s) 378, 463, 505, 721
DdeI CTNAG 3 cut(s) 412, 608, 1342
EaeI YGGCCR 1 cut(s) 1122
EciI GGCGGA 1 cut(s) 397
Ecl136II GAGCTC 1 cut(s) 349
Eco24I GRGCYC 1 cut(s) 351
Eco31I GGTCTC 1 cut(s) 788
Eco47I GGWCC 2 cut(s) 159, 571
Eco53kI GAGCTC 1 cut(s) 349
Eco57I CTGAAG 1 cut(s) 1295
EcoICRI GAGCTC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 934
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 3 cut(s) 548, 613, 1114
EcoT38I GRGCYC 1 cut(s) 351
FaeI CATG 5 cut(s) 40, 74, 169, 355, 1387
FaqI GGGAC 3 cut(s) 172, 209, 557
FatI CATG 5 cut(s) 36, 70, 165, 351, 1383
FauNDI CATATG 2 cut(s) 1174, 1310
Fnu4HI GCNGC 6 cut(s) 117, 120, 204, 217, 966, 1408
FokI GGATG 1 cut(s) 1098
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 6 cut(s) 117, 120, 204, 217, 966, 1408
FspBI CTAG 1 cut(s) 584
GlaI GCGC 4 cut(s) 89, 202, 314, 579
GluI GCNGC 6 cut(s) 117, 120, 204, 217, 966, 1408
GsuI CTGGAG 1 cut(s) 1380
HaeIII GGCC 5 cut(s) 11, 83, 216, 1124, 1347
HapII CCGG 7 cut(s) 85, 156, 472, 1091, 1125, 1373, 1452
HhaI GCGC 4 cut(s) 90, 203, 315, 580
Hin1II CATG 5 cut(s) 40, 74, 169, 355, 1387
Hin6I GCGC 4 cut(s) 88, 201, 313, 578
HinP1I GCGC 4 cut(s) 88, 201, 313, 578
HincII GTYRAC 1 cut(s) 1051
HindII GTYRAC 1 cut(s) 1051
HindIII AAGCTT 1 cut(s) 432
HinfI GANTC 6 cut(s) 4, 15, 262, 296, 404, 1128
HpaII CCGG 7 cut(s) 85, 156, 472, 1091, 1125, 1373, 1452
HphI GGTGA 2 cut(s) 877, 1466
Hpy166II GTNNAC 9 cut(s) 177, 187, 463, 505, 523, 721, 1051, 1321, 1378
Hpy188I TCNGA 6 cut(s) 301, 344, 360, 978, 1181, 1397
Hpy188III TCNNGA 5 cut(s) 109, 259, 271, 557, 760
Hpy8I GTNNAC 9 cut(s) 177, 187, 463, 505, 523, 721, 1051, 1321, 1378
HpyAV CCTTC 8 cut(s) 40, 77, 289, 812, 862, 885, 1017, 1181
HpyCH4III ACNGT 5 cut(s) 331, 377, 498, 1222, 1293
HpyCH4IV ACGT 2 cut(s) 723, 732
HpyCH4V TGCA 6 cut(s) 61, 430, 1232, 1259, 1367, 1383
HpyF10VI GCNNNNNNNGC 7 cut(s) 310, 422, 427, 431, 586, 1364, 1407
HpyF3I CTNAG 3 cut(s) 412, 608, 1342
HpySE526I ACGT 2 cut(s) 723, 732
Hsp92II CATG 5 cut(s) 40, 74, 169, 355, 1387
HspAI GCGC 4 cut(s) 88, 201, 313, 578
KspI CCGCGG 1 cut(s) 120
LmnI GCTCC 3 cut(s) 301, 856, 1304
Lsp1109I GCAGC 2 cut(s) 977, 1419
LweI GCATC 2 cut(s) 982, 1345
MaeI CTAG 1 cut(s) 584
MaeII ACGT 2 cut(s) 723, 732
MaeIII GTNAC 2 cut(s) 988, 1147
MboII GAAGA 5 cut(s) 40, 469, 684, 1325, 1384
MflI RGATCY 3 cut(s) 63, 553, 1168
MhlI GDGCHC 1 cut(s) 351
MlyI GAGTC 1 cut(s) 271
MmeI TCCRAC 1 cut(s) 1159
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 2 cut(s) 726, 1248
MslI CAYNNNNRTG 2 cut(s) 170, 182
MspA1I CMGCKG 2 cut(s) 119, 809
MspCI CTTAAG 1 cut(s) 1247
MspI CCGG 7 cut(s) 85, 156, 472, 1091, 1125, 1373, 1452
MspR9I CCNGG 7 cut(s) 85, 157, 550, 615, 1092, 1116, 1452
Mva1269I GAATGC 3 cut(s) 63, 104, 1261
MvaI CCWGG 3 cut(s) 550, 615, 1116
MvnI CGCG 4 cut(s) 90, 119, 219, 315
MwoI GCNNNNNNNGC 7 cut(s) 310, 422, 427, 431, 586, 1364, 1407
NciI CCSGG 4 cut(s) 85, 157, 1092, 1452
NdeI CATATG 2 cut(s) 1174, 1310
NlaIII CATG 5 cut(s) 40, 74, 169, 355, 1387
NlaIV GGNNCC 6 cut(s) 160, 269, 573, 858, 1001, 1306
OliI CACNNNNGTG 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 729
PctI GAATGC 3 cut(s) 63, 104, 1261
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 5 cut(s) 4, 15, 296, 404, 1128
PfoI TCCNGGA 1 cut(s) 155
PinAI ACCGGT 1 cut(s) 471
PkrI GCNGC 6 cut(s) 118, 121, 205, 218, 967, 1409
Ple19I CGATCG 1 cut(s) 140
PleI GAGTC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 351
Psp5II RGGWCCY 1 cut(s) 159
Psp6I CCWGG 3 cut(s) 548, 613, 1114
PspGI CCWGG 3 cut(s) 548, 613, 1114
PspN4I GGNNCC 6 cut(s) 160, 269, 573, 858, 1001, 1306
PspPI GGNCC 5 cut(s) 10, 82, 159, 214, 571
PspPPI RGGWCCY 1 cut(s) 159
PstNI CAGNNNCTG 1 cut(s) 976
PsuI RGATCY 3 cut(s) 63, 553, 1168
PvuI CGATCG 1 cut(s) 140
PvuII CAGCTG 1 cut(s) 809
RsaI GTAC 4 cut(s) 379, 464, 506, 722
RsaNI GTAC 4 cut(s) 378, 463, 505, 721
RseI CAYNNNNRTG 2 cut(s) 170, 182
SacI GAGCTC 1 cut(s) 351
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 2 cut(s) 726, 1248
SatI GCNGC 6 cut(s) 117, 120, 204, 217, 966, 1408
Sau96I GGNCC 5 cut(s) 10, 82, 159, 214, 571
SchI GAGTC 1 cut(s) 271
ScrFI CCNGG 7 cut(s) 85, 157, 550, 615, 1092, 1116, 1452
SduI GDGCHC 1 cut(s) 351
SfaNI GCATC 2 cut(s) 982, 1345
SfcI CTRYAG 1 cut(s) 393
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 2 cut(s) 159, 571
SmiMI CAYNNNNRTG 2 cut(s) 170, 182
SmlI CTYRAG 3 cut(s) 485, 590, 1247
SmoI CTYRAG 3 cut(s) 485, 590, 1247
SpeI ACTAGT 1 cut(s) 583
SsiI CCGC 6 cut(s) 117, 119, 204, 217, 408, 1405
SspMI CTAG 1 cut(s) 584
SstI GAGCTC 1 cut(s) 351
StyD4I CCNGG 7 cut(s) 83, 155, 548, 613, 1090, 1114, 1450
TaaI ACNGT 5 cut(s) 331, 377, 498, 1222, 1293
TaiI ACGT 2 cut(s) 726, 735
TaqI TCGA 9 cut(s) 128, 140, 231, 534, 558, 597, 835, 1020, 1155
TaqII GACCGA 1 cut(s) 559
TatI WGTACW 1 cut(s) 462
TauI GCSGC 4 cut(s) 119, 122, 206, 219
TfiI GAWTC 5 cut(s) 4, 15, 296, 404, 1128
Tru1I TTAA 2 cut(s) 726, 1248
Tru9I TTAA 2 cut(s) 726, 1248
TseI GCWGC 2 cut(s) 965, 1407
TspDTI ATGAA 3 cut(s) 59, 1200, 1268
TspGWI ACGGA 2 cut(s) 257, 1301
Vha464I CTTAAG 1 cut(s) 1247
VpaK11BI GGWCC 2 cut(s) 159, 571
XagI CCTNNNNNAGG 1 cut(s) 934
XapI RAATTY 1 cut(s) 322
XcmI CCANNNNNNNNNTGG 2 cut(s) 1315, 1355
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.