RchiOBHm_Chr3g0469451

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007950
Physical Location & Seq
Forward (+)
15228509 .. 15230880
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43529

Sequence Viewer

Length: 1674 bp
ATGGATCGGGGTGTGTGGTACGATACAAAGCCTGCAAAGGTCTCAAAGAGGGTGTGGTTAGTTATTCATAAATCAATGGTACTCCTCGTGGGTGGGAATCACACCTCCGCCAGAATTTTTTGTTTTTGTTTTTTGCAATCAAACAAAATTCGCAAACTCTGCTCTCTGAGAAGAGAGGATTCGAAACCGCAATCGTTCGGTTTCGCCCTGTTCTTTCGCGGCCGAATCGCGAAAGAAAGAGCACAAGTCATGGATTTGAAGCTCAAGAAACTCCAGGTCCTTCACGGATCTGTGGCCCGGCGCGTGTTTTACCGCTCGTTCTTTATCGCCGTGGCTATGTCTGTAATTCCCCTGGTTCACATTCTGTCCGGTTCTTACATGAAAATGTTCAACTTTGTGAACACTATTGACTGTGCCTCCGGAATGGGCCGTGACAATGTCAACTTGGACCCGGGCAAGTTCATCTTTCAGACCCGGTTTCTGAATCCCATTTGGGGTACTTTCGATTTGGCGCAGTGCAAGCAAGATGTGAACTTGACTGTCAGTGTGGTCAGAGAGCTAATGGGCGAGCAGCTTTTGAACGGTGATAAGGCTCTCTGTGTCGGTGAAGGATCGGCCTCGGCCGTGATGGCGTTGCAAGATTTGGGGTTTCCCGATGCTCGTGGTGTTTATGAACACCGATTCTTCTCCCTCAAGCGCAAACAGTTTGTGTATGAGATTTACTATGAGGACAAGTCTTTCGATTTCGTGTTCTGCAGGGATCTAGACAAGGTTTCAGTCCCTGCTCTGCTTGTGCTCGAGATTGAGCGCGTTCTTAGCCCAGGTGGAATTGGGGCTATGCTTGTTGGTAGCAGTGGTTTGGCTCCGAATAGCCTGATTAGGTCTGCTACCCCAATTTCTTCATTGCTGAAGAGCTCCAGTGTCGTGCATGTTAATTATATCAGCAATTTCACACTGGTTGTGTTCAGGAAGAGTTACGAAAATGTTGGTCTATTTGAGCAGTATCGCCTTCCGGCTGACTGCCAATCCCTCACAAACAATAGACCTTTGATCGAGAAAATGGAGCCTCTTGTGAAGGAAAAGCCAGTGGAGGACAGGAAGATGGCTACTTATCTGCCTAATTTTATTGATGTTTCCTCAAAGAAGCGGTTGGTCTATATCGATATTGGGGCAGCAGAGCACCTGAAATCAAATGTCACAGACTGGTTCCTGCCTTCTTATCCGATTACACACAGAGCTTTCCATGTCTATTTTGTTGACCACAACACTTCTGTGCTATTGTCTTATGTCAAAAAGCCAGGAATCACCTTCGTTTATCATCCAGGGCTGGCTGGAATTAAGGACAAGGACAATGTTAACATCGAAGCAGATGCAGAGCCCTATGTCGCAGATGAAGGGTTTGATTTTCTTGTTTGGTTTAAAGAAACAGTGCAGCATGCCGAGTTTGTGGTGCTGAAGATGAATGCAGGCAGTGTAGAATTGAAGTTCCTGAGGGATCTGTTTGAGAGTGGAGCCATATGCTTTGTCGACGAGCTGTTTCTTCACTGCTCTGGCCACATAGACGGGGAAGGTCCAATGCCGGTAGACTGCATGGACATCTTCAAGAGCCTCCGCAGCAGTGGTGTGTATGTGCATCAGTGGTGGGGAGAAGACAACCCCGTCGATGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

557

Amino Acids

63.09

Weight (kDa)

8.34

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 462 - 548 1e-12 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1658
AccBSI CCGCTC 1 cut(s) 315
AccI GTMKAC 2 cut(s) 1527, 1584
AccII CGCG 4 cut(s) 219, 230, 303, 810
AccIII TCCGGA 1 cut(s) 419
AciI CCGC 6 cut(s) 108, 188, 219, 313, 1147, 1612
AclWI GGATC 5 cut(s) 12, 295, 619, 768, 1503
AcoI YGGCCR 3 cut(s) 220, 621, 1552
AcsI RAATTY 2 cut(s) 114, 147
AcuI CTGAAG 2 cut(s) 929, 1475
AfaI GTAC 3 cut(s) 20, 81, 499
AfiI CCNNNNNNNGG 1 cut(s) 494
AgsI TTSAA 5 cut(s) 259, 391, 580, 1483, 1603
AjnI CCWGG 5 cut(s) 273, 351, 820, 1297, 1321
AleI CACNNNNGTG 1 cut(s) 1271
AluBI AGCT 6 cut(s) 262, 559, 574, 915, 1238, 1534
AluI AGCT 6 cut(s) 262, 559, 574, 915, 1238, 1534
Alw21I GWGCWC 4 cut(s) 244, 798, 917, 1182
Alw26I GTCTC 1 cut(s) 46
AlwI GGATC 5 cut(s) 12, 295, 619, 768, 1503
AlwNI CAGNNNCTG 1 cut(s) 782
Ama87I CYCGRG 2 cut(s) 451, 797
Aor13HI TCCGGA 1 cut(s) 419
AoxI GGCC 6 cut(s) 220, 294, 427, 615, 621, 1552
ApeKI GCWGC 4 cut(s) 571, 1172, 1432, 1614
ApoI RAATTY 2 cut(s) 114, 147
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 4 cut(s) 303, 514, 699, 810
AspS9I GGNCC 5 cut(s) 277, 295, 427, 448, 1571
AsuC2I CCSGG 4 cut(s) 298, 452, 453, 475
AsuHPI GGTGA 3 cut(s) 596, 617, 1297
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 2 cut(s) 451, 797
AvaII GGWCC 3 cut(s) 277, 448, 1571
AxyI CCTNAGG 1 cut(s) 1490
BalI TGGCCA 1 cut(s) 1554
BanII GRGCYC 2 cut(s) 917, 1380
BauI CACGAG 2 cut(s) 86, 660
BbsI GAAGAC 1 cut(s) 1656
Bbv12I GWGCWC 4 cut(s) 244, 798, 917, 1182
BbvI GCAGC 4 cut(s) 583, 1184, 1444, 1626
BccI CCATC 2 cut(s) 622, 1096
BceAI ACGGC 3 cut(s) 314, 414, 608
BcgI CGANNNNNNTGC 2 cut(s) 1352, 1386
BciT130I CCWGG 5 cut(s) 275, 353, 822, 1299, 1323
BcnI CCSGG 4 cut(s) 298, 452, 453, 475
BcoDI GTCTC 1 cut(s) 46
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 629
BisI GCNGC 5 cut(s) 220, 572, 1173, 1433, 1615
BlsI GCNGC 5 cut(s) 221, 573, 1174, 1434, 1616
Bme1390I CCNGG 9 cut(s) 275, 298, 353, 452, 453, 475, 822, 1299, 1323
Bme18I GGWCC 3 cut(s) 277, 448, 1571
BmeT110I CYCGRG 2 cut(s) 451, 797
BmgT120I GGNCC 5 cut(s) 277, 295, 427, 448, 1571
BmiI GGNNCC 5 cut(s) 450, 864, 1065, 1208, 1513
BmrFI CCNGG 9 cut(s) 275, 298, 353, 452, 453, 475, 822, 1299, 1323
BmsI GCATC 3 cut(s) 646, 1360, 1642
BpiI GAAGAC 1 cut(s) 1656
BpmI CTGGAG 2 cut(s) 257, 901
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 2 cut(s) 248, 677
BpuMI CCSGG 4 cut(s) 298, 452, 453, 475
Bsa29I ATCGAT 1 cut(s) 1161
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 6 cut(s) 330, 351, 451, 618, 820, 1322
BsaWI WCCGGW 2 cut(s) 368, 419
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse118I RCCGGY 1 cut(s) 1579
Bse1I ACTGG 4 cut(s) 918, 960, 1085, 1208
Bse21I CCTNAGG 1 cut(s) 1490
Bse3DI GCAATG 1 cut(s) 902
BseAI TCCGGA 1 cut(s) 419
BseBI CCWGG 5 cut(s) 275, 353, 822, 1299, 1323
BseCI ATCGAT 1 cut(s) 1161
BseDI CCNNGG 6 cut(s) 330, 351, 451, 618, 820, 1322
BseGI GGATG 1 cut(s) 1318
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 902
BseMII CTCAG 2 cut(s) 158, 1481
BseNI ACTGG 4 cut(s) 918, 960, 1085, 1208
BseRI GAGGAG 1 cut(s) 74
BseX3I CGGCCG 2 cut(s) 220, 621
BseXI GCAGC 4 cut(s) 583, 1184, 1444, 1626
BsgI GTGCAG 1 cut(s) 1451
Bsh1236I CGCG 4 cut(s) 219, 230, 303, 810
Bsh1285I CGRYCG 2 cut(s) 223, 624
BshFI GGCC 6 cut(s) 222, 296, 429, 617, 623, 1554
BshVI ATCGAT 1 cut(s) 1161
BsiEI CGRYCG 2 cut(s) 223, 624
BsiHKAI GWGCWC 4 cut(s) 244, 798, 917, 1182
BsiHKCI CYCGRG 2 cut(s) 451, 797
BsiSI CCGG 7 cut(s) 298, 369, 420, 452, 475, 1013, 1580
BslFI GGGAC 1 cut(s) 764
BslI CCNNNNNNNGG 1 cut(s) 494
BsmAI GTCTC 1 cut(s) 46
BsmFI GGGAC 1 cut(s) 764
BsmI GAATGC 1 cut(s) 1468
BsnI GGCC 6 cut(s) 222, 296, 429, 617, 623, 1554
Bso31I GGTCTC 1 cut(s) 46
BsoBI CYCGRG 2 cut(s) 451, 797
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 5 cut(s) 244, 798, 917, 1182, 1380
Bsp13I TCCGGA 1 cut(s) 419
Bsp143I GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
Bsp68I TCGCGA 1 cut(s) 230
BspACI CCGC 6 cut(s) 108, 188, 219, 313, 1147, 1612
BspANI GGCC 6 cut(s) 222, 296, 429, 617, 623, 1554
BspCNI CTCAG 2 cut(s) 159, 1482
BspDI ATCGAT 1 cut(s) 1161
BspEI TCCGGA 1 cut(s) 419
BspFNI CGCG 4 cut(s) 219, 230, 303, 810
BspLI GGNNCC 5 cut(s) 450, 864, 1065, 1208, 1513
BspMAI CTGCAG 1 cut(s) 758
BspPI GGATC 5 cut(s) 12, 295, 619, 768, 1503
BspQI GCTCTTC 1 cut(s) 905
BspT104I TTCGAA 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 902
BsrFI RCCGGY 1 cut(s) 1579
BsrI ACTGG 4 cut(s) 918, 960, 1085, 1208
BssAI RCCGGY 1 cut(s) 1579
BssECI CCNNGG 6 cut(s) 330, 351, 451, 618, 820, 1322
BssMI GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
BssSI CACGAG 2 cut(s) 86, 660
Bst2BI CACGAG 2 cut(s) 86, 660
Bst2UI CCWGG 5 cut(s) 275, 353, 822, 1299, 1323
Bst4CI ACNGT 5 cut(s) 413, 541, 584, 705, 1429
Bst6I CTCTTC 3 cut(s) 166, 905, 965
BstAPI GCANNNNNTGC 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 6 cut(s) 33, 521, 569, 1329, 1437, 1468
BstDEI CTNAG 3 cut(s) 167, 815, 1490
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 1318
BstFNI CGCG 4 cut(s) 219, 230, 303, 810
BstHHI GCGC 4 cut(s) 303, 514, 699, 810
BstKTI GATC 6 cut(s) 7, 290, 614, 763, 1053, 1498
BstMAI GTCTC 1 cut(s) 46
BstMBI GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
BstMCI CGRYCG 2 cut(s) 223, 624
BstMWI GCNNNNNNNGC 5 cut(s) 159, 520, 629, 816, 1614
BstNI CCWGG 5 cut(s) 275, 353, 822, 1299, 1323
BstNSI RCATGY 2 cut(s) 932, 1439
BstSCI CCNGG 9 cut(s) 273, 296, 351, 450, 451, 473, 820, 1297, 1321
BstSFI CTRYAG 1 cut(s) 754
BstUI CGCG 4 cut(s) 219, 230, 303, 810
BstV1I GCAGC 4 cut(s) 583, 1184, 1444, 1626
BstV2I GAAGAC 1 cut(s) 1656
BstX2I RGATCY 3 cut(s) 287, 760, 1495
BstYI RGATCY 3 cut(s) 287, 760, 1495
BstZI CGGCCG 2 cut(s) 220, 621
Bsu15I ATCGAT 1 cut(s) 1161
Bsu36I CCTNAGG 1 cut(s) 1490
BsuRI GGCC 6 cut(s) 222, 296, 429, 617, 623, 1554
BsuTUI ATCGAT 1 cut(s) 1161
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 1318
BtsI GCAGTG 5 cut(s) 521, 859, 1477, 1543, 1624
BtuMI TCGCGA 1 cut(s) 230
Cac8I GCNNGC 6 cut(s) 33, 521, 569, 1329, 1437, 1468
CaiI CAGNNNCTG 1 cut(s) 782
CfoI GCGC 4 cut(s) 303, 514, 699, 810
Cfr10I RCCGGY 1 cut(s) 1579
Cfr13I GGNCC 5 cut(s) 277, 295, 427, 448, 1571
Cfr9I CCCGGG 1 cut(s) 451
ClaI ATCGAT 1 cut(s) 1161
Csp6I GTAC 3 cut(s) 19, 80, 498
CviAII CATG 6 cut(s) 250, 379, 929, 1244, 1436, 1591
CviQI GTAC 3 cut(s) 19, 80, 498
DdeI CTNAG 3 cut(s) 167, 815, 1490
DpnI GATC 6 cut(s) 6, 289, 613, 762, 1052, 1497
DpnII GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
DraI TTTAAA 1 cut(s) 1420
DrdI GACNNNNNNGTC 1 cut(s) 1658
DseDI GACNNNNNNGTC 1 cut(s) 1658
EaeI YGGCCR 3 cut(s) 220, 621, 1552
EagI CGGCCG 2 cut(s) 220, 621
Eam1104I CTCTTC 3 cut(s) 166, 905, 965
EarI CTCTTC 3 cut(s) 166, 905, 965
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 915
EclXI CGGCCG 2 cut(s) 220, 621
Eco24I GRGCYC 2 cut(s) 917, 1380
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 3 cut(s) 277, 448, 1571
Eco52I CGGCCG 2 cut(s) 220, 621
Eco53kI GAGCTC 1 cut(s) 915
Eco57I CTGAAG 2 cut(s) 929, 1475
Eco81I CCTNAGG 1 cut(s) 1490
Eco88I CYCGRG 2 cut(s) 451, 797
EcoICRI GAGCTC 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 277
EcoRII CCWGG 5 cut(s) 273, 351, 820, 1297, 1321
EcoT38I GRGCYC 2 cut(s) 917, 1380
FaeI CATG 6 cut(s) 253, 382, 932, 1247, 1439, 1594
FalI AAGNNNNNCTT 2 cut(s) 449, 481
FaqI GGGAC 1 cut(s) 764
FatI CATG 6 cut(s) 249, 378, 928, 1243, 1435, 1590
FauNDI CATATG 1 cut(s) 1517
FblI GTMKAC 2 cut(s) 1527, 1584
Fnu4HI GCNGC 5 cut(s) 220, 572, 1173, 1433, 1615
FokI GGATG 1 cut(s) 1305
FriOI GRGCYC 2 cut(s) 917, 1380
Fsp4HI GCNGC 5 cut(s) 220, 572, 1173, 1433, 1615
FspBI CTAG 1 cut(s) 764
GlaI GCGC 4 cut(s) 302, 513, 698, 809
GluI GCNGC 5 cut(s) 220, 572, 1173, 1433, 1615
GsuI CTGGAG 2 cut(s) 257, 901
HaeIII GGCC 6 cut(s) 222, 296, 429, 617, 623, 1554
HapII CCGG 7 cut(s) 298, 369, 420, 452, 475, 1013, 1580
HhaI GCGC 4 cut(s) 303, 514, 699, 810
Hin1II CATG 6 cut(s) 253, 382, 932, 1247, 1439, 1594
Hin6I GCGC 4 cut(s) 301, 512, 697, 808
HinP1I GCGC 4 cut(s) 301, 512, 697, 808
HincII GTYRAC 4 cut(s) 442, 1258, 1357, 1528
HindII GTYRAC 4 cut(s) 442, 1258, 1357, 1528
HinfI GANTC 6 cut(s) 97, 179, 225, 484, 681, 1302
HpaI GTTAAC 1 cut(s) 1357
HpaII CCGG 7 cut(s) 298, 369, 420, 452, 475, 1013, 1580
HphI GGTGA 3 cut(s) 596, 617, 1297
Hpy166II GTNNAC 8 cut(s) 358, 400, 442, 532, 1258, 1357, 1528, 1585
Hpy188I TCNGA 6 cut(s) 168, 471, 483, 554, 867, 1224
Hpy8I GTNNAC 8 cut(s) 358, 400, 442, 532, 1258, 1357, 1528, 1585
Hpy99I CGWCG 2 cut(s) 1532, 1664
HpyAV CCTTC 8 cut(s) 290, 602, 1019, 1069, 1224, 1318, 1388, 1562
HpyCH4III ACNGT 5 cut(s) 413, 541, 584, 705, 1429
HpyF10VI GCNNNNNNNGC 5 cut(s) 159, 520, 629, 816, 1614
HpyF3I CTNAG 3 cut(s) 167, 815, 1490
Hsp92II CATG 6 cut(s) 253, 382, 932, 1247, 1439, 1594
HspAI GCGC 4 cut(s) 301, 512, 697, 808
Kpn2I TCCGGA 1 cut(s) 419
KspAI GTTAAC 1 cut(s) 1357
Kzo9I GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
LguI GCTCTTC 1 cut(s) 905
LmnI GCTCC 4 cut(s) 868, 920, 1063, 1511
Lsp1109I GCAGC 4 cut(s) 583, 1184, 1444, 1626
LweI GCATC 3 cut(s) 646, 1360, 1642
MaeI CTAG 1 cut(s) 764
MaeIII GTNAC 3 cut(s) 431, 974, 1195
MalI GATC 6 cut(s) 6, 289, 613, 762, 1052, 1497
MbiI CCGCTC 1 cut(s) 315
MboI GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
MflI RGATCY 3 cut(s) 287, 760, 1495
MhlI GDGCHC 5 cut(s) 244, 798, 917, 1182, 1380
MlsI TGGCCA 1 cut(s) 1554
MluNI TGGCCA 1 cut(s) 1554
Mox20I TGGCCA 1 cut(s) 1554
MroI TCCGGA 1 cut(s) 419
MroXI GAANNNNTTC 1 cut(s) 386
MscI TGGCCA 1 cut(s) 1554
MseI TTAA 4 cut(s) 933, 1338, 1356, 1419
MslI CAYNNNNRTG 2 cut(s) 383, 1271
Msp20I TGGCCA 1 cut(s) 1554
MspI CCGG 7 cut(s) 298, 369, 420, 452, 475, 1013, 1580
MspR9I CCNGG 9 cut(s) 275, 298, 353, 452, 453, 475, 822, 1299, 1323
Mva1269I GAATGC 1 cut(s) 1468
MvaI CCWGG 5 cut(s) 275, 353, 822, 1299, 1323
MvnI CGCG 4 cut(s) 219, 230, 303, 810
MwoI GCNNNNNNNGC 5 cut(s) 159, 520, 629, 816, 1614
NciI CCSGG 4 cut(s) 298, 452, 453, 475
NdeI CATATG 1 cut(s) 1517
NdeII GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
NlaIII CATG 6 cut(s) 253, 382, 932, 1247, 1439, 1594
NlaIV GGNNCC 5 cut(s) 450, 864, 1065, 1208, 1513
NmeAIII GCCGAG 2 cut(s) 599, 1465
NmuCI GTSAC 2 cut(s) 431, 1195
NruI TCGCGA 1 cut(s) 230
NspI RCATGY 2 cut(s) 932, 1439
NspV TTCGAA 1 cut(s) 182
OliI CACNNNNGTG 1 cut(s) 1271
PaeI GCATGC 1 cut(s) 1439
PaeR7I CTCGAG 1 cut(s) 797
PciSI GCTCTTC 1 cut(s) 905
PctI GAATGC 1 cut(s) 1468
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 6 cut(s) 97, 179, 225, 484, 681, 1302
PflFI GACNNNGTC 1 cut(s) 437
PkrI GCNGC 5 cut(s) 221, 573, 1174, 1434, 1616
PpuMI RGGWCCY 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 917
Psp5II RGGWCCY 1 cut(s) 277
Psp6I CCWGG 5 cut(s) 273, 351, 820, 1297, 1321
PspGI CCWGG 5 cut(s) 273, 351, 820, 1297, 1321
PspN4I GGNNCC 5 cut(s) 450, 864, 1065, 1208, 1513
PspPI GGNCC 5 cut(s) 277, 295, 427, 448, 1571
PspPPI RGGWCCY 1 cut(s) 277
PstI CTGCAG 1 cut(s) 758
PstNI CAGNNNCTG 1 cut(s) 782
PsuI RGATCY 3 cut(s) 287, 760, 1495
PsyI GACNNNGTC 1 cut(s) 437
RruI TCGCGA 1 cut(s) 230
RsaI GTAC 3 cut(s) 20, 81, 499
RsaNI GTAC 3 cut(s) 19, 80, 498
RseI CAYNNNNRTG 2 cut(s) 383, 1271
SacI GAGCTC 1 cut(s) 917
SalI GTCGAC 1 cut(s) 1526
SapI GCTCTTC 1 cut(s) 905
SaqAI TTAA 4 cut(s) 933, 1338, 1356, 1419
SatI GCNGC 5 cut(s) 220, 572, 1173, 1433, 1615
Sau3AI GATC 6 cut(s) 4, 287, 611, 760, 1050, 1495
Sau96I GGNCC 5 cut(s) 277, 295, 427, 448, 1571
ScrFI CCNGG 9 cut(s) 275, 298, 353, 452, 453, 475, 822, 1299, 1323
SduI GDGCHC 5 cut(s) 244, 798, 917, 1182, 1380
SfaNI GCATC 3 cut(s) 646, 1360, 1642
SfcI CTRYAG 1 cut(s) 754
Sfr274I CTCGAG 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 3 cut(s) 277, 448, 1571
SlaI CTCGAG 1 cut(s) 797
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 2 cut(s) 383, 1271
SmlI CTYRAG 3 cut(s) 263, 692, 797
SmoI CTYRAG 3 cut(s) 263, 692, 797
SphI GCATGC 1 cut(s) 1439
SsiI CCGC 6 cut(s) 108, 188, 219, 313, 1147, 1612
SspMI CTAG 1 cut(s) 764
SstI GAGCTC 1 cut(s) 917
StyD4I CCNGG 9 cut(s) 273, 296, 351, 450, 451, 473, 820, 1297, 1321
TaaI ACNGT 5 cut(s) 413, 541, 584, 705, 1429
TaqI TCGA 9 cut(s) 182, 504, 741, 798, 1053, 1161, 1362, 1527, 1662
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 6 cut(s) 97, 179, 225, 484, 681, 1302
Tru1I TTAA 4 cut(s) 933, 1338, 1356, 1419
Tru9I TTAA 4 cut(s) 933, 1338, 1356, 1419
TseFI GTSAC 2 cut(s) 431, 1195
TseI GCWGC 4 cut(s) 571, 1172, 1432, 1614
Tsp45I GTSAC 2 cut(s) 431, 1195
TspDTI ATGAA 7 cut(s) 56, 395, 451, 687, 891, 1407, 1475
TspGWI ACGGA 1 cut(s) 300
TspMI CCCGGG 1 cut(s) 451
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 3 cut(s) 277, 448, 1571
XapI RAATTY 2 cut(s) 114, 147
XbaI TCTAGA 1 cut(s) 763
XceI RCATGY 2 cut(s) 932, 1439
XhoI CTCGAG 1 cut(s) 797
XmaI CCCGGG 1 cut(s) 451
XmiI GTMKAC 2 cut(s) 1527, 1584
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.