Rroxscaffold_6G00411880

Methyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007950
Physical Location & Seq
Reverse (-)
34494775 .. 34497067
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00411880.1

Sequence Viewer

Length: 1425 bp
ATGGATTTGAAGCTCAAGAAACTCCAGATCCTTCACGGATCTCTAGCCCGGCGCGTGTATTACCGCACTTTCTTTATCGCCGTGGCTATGTCTGTAATTCCCTTGGTTCACATTTTGTCCGGTTCTTACATGAAAATGTTCAACTTTGTAAACTTTACGGACTGTGCCTCCGAATTGGGCCGTGACAATGTCAACTTGGACCCGGGCAAGTTCATCTTTCAGACCCGGTTTCTGAATCCCATTTGGGGTACTTTCGATTCGGAGCAGTGCAAGCAAGATGTGAACTTGACTGTCAGTGTGGTCAGAGAGCTAATGGGCGAGCAGCTTTTGAACGGTGATAAAGCTCTCTGTGTTGGTGAAGGATCGGCCTCGGCCGTGATGGCGTTGCAAGATTTGGGGTTTCCCAATGCTCGTGGTGTTTATGAACACCGATTCTTCTCCCTCAAGCGCAAACAGTTTGTGTATGAGATTTACTATGAGGACAAGTCTTTCGATTTCGTGTTCTGCAGGGATCTAGACAAGGTTTCAGTCCCTGCTCTGCTTGTGCTCGAGATTGAGCGCGTTCTTAGCCCAGGTGGAATTGGGGCTATGCTTGTTGGTAGCAGTGGTTTGGCTCCGAATAGCCTGATTAGGTCTGCTACCCCAATTTCTTCATTGCTGAAGAGCTCCAGTGTCGTGCATGTTAATTATATCAGCAATTTCACACTGGTTGTGTTCAGGAAGAGTTACGAAAATGCTGGTCTATTTGAGCAGTATCGCCTTCCGGCCGACTGCCAATCCCTCACAAACAATAGACCTTTGATCGAGAAAATGGAGCCTCTTGTGAAGGAAAAGCCAGTGGAGGACGGGAAGATGGCTACTTATCTGCCTAATTTTATTGATGTTTCCTCAAAGAAGCGGTTGGTCTATATCGACATTGGGGCAGCAGAGCACCTGAAATCAAATGCCACAGACTGGTTCCTGCCTTCTTATCCGATTACACACAGAGCTTTCCATGTCTATTTTGTTGACCACAACACTTCTGTGCTATTGTCCTATGTCAAAAAGCCGGGAATCACCTTCGTTTATCATCCAGGGCTGGCAGGAATTAAGGACAAGAACAATGTTAACATCGAAGCAGATGCAGAGCCCTATGTCGCAGATGAAGGGTTTGATTTTCTTGTTTGGTTTAAAGAAACAGTGCAGCATGCCGAGTTTGTGGTGCTGAAGATGAATGCAGGCAGTGTAGAATTGAAGTTCCTGAGGGATCTATTTGAGAGTGGAGCCATATGCTTTGTCGACGAGCTGTTTCTTCACTGCTCTAGCCACATAGACGGGGAAGGTCCAATGCCGGTAGACTGCATGGACATCTTCAAGAGCCTCCGCAGCAGTGGTGTGTATGTGCATCAGTGGTGGGGAGAAGACAACCCCGTCGATGTTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

474

Amino Acids

53.48

Weight (kDa)

5.84

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 377 - 465 2.9e-13 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1409
AccB7I CCANNNNNTGG 1 cut(s) 954
AccI GTMKAC 2 cut(s) 1278, 1335
AccII CGCG 2 cut(s) 54, 561
AciI CCGC 3 cut(s) 64, 898, 1363
AclWI GGATC 5 cut(s) 22, 46, 370, 519, 1254
AcoI YGGCCR 2 cut(s) 372, 765
AcuI CTGAAG 2 cut(s) 680, 1226
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 2 cut(s) 245, 954
AgsI TTSAA 5 cut(s) 10, 142, 331, 1234, 1354
AjnI CCWGG 2 cut(s) 571, 1072
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 7 cut(s) 13, 310, 325, 344, 666, 989, 1285
AluI AGCT 7 cut(s) 13, 310, 325, 344, 666, 989, 1285
Alw21I GWGCWC 3 cut(s) 549, 668, 933
AlwI GGATC 5 cut(s) 22, 46, 370, 519, 1254
AlwNI CAGNNNCTG 1 cut(s) 533
Ama87I CYCGRG 2 cut(s) 202, 548
AoxI GGCC 4 cut(s) 178, 366, 372, 765
ApeKI GCWGC 4 cut(s) 322, 923, 1183, 1365
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
Asp700I GAANNNNTTC 1 cut(s) 137
AspLEI GCGC 3 cut(s) 54, 450, 561
AspS9I GGNCC 3 cut(s) 178, 199, 1322
AsuC2I CCSGG 5 cut(s) 49, 203, 204, 226, 1050
AsuHPI GGTGA 3 cut(s) 347, 368, 1048
AvaI CYCGRG 2 cut(s) 202, 548
AvaII GGWCC 2 cut(s) 199, 1322
AxyI CCTNAGG 1 cut(s) 1241
BanII GRGCYC 2 cut(s) 668, 1131
BauI CACGAG 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 1407
Bbv12I GWGCWC 3 cut(s) 549, 668, 933
BbvI GCAGC 4 cut(s) 334, 935, 1195, 1377
BccI CCATC 2 cut(s) 373, 847
BceAI ACGGC 3 cut(s) 65, 165, 359
BcgI CGANNNNNNTGC 2 cut(s) 1103, 1137
BciT130I CCWGG 2 cut(s) 573, 1074
BcnI CCSGG 5 cut(s) 49, 203, 204, 226, 1050
BfaI CTAG 3 cut(s) 44, 515, 1302
BfmI CTRYAG 1 cut(s) 505
BglI GCCNNNNNGGC 1 cut(s) 380
BisI GCNGC 4 cut(s) 323, 924, 1184, 1366
BlsI GCNGC 4 cut(s) 324, 925, 1185, 1367
Bme1390I CCNGG 7 cut(s) 49, 203, 204, 226, 573, 1050, 1074
Bme18I GGWCC 2 cut(s) 199, 1322
BmeT110I CYCGRG 2 cut(s) 202, 548
BmgT120I GGNCC 3 cut(s) 178, 199, 1322
BmiI GGNNCC 5 cut(s) 201, 615, 816, 959, 1264
BmrFI CCNGG 7 cut(s) 49, 203, 204, 226, 573, 1050, 1074
BmsI GCATC 2 cut(s) 1111, 1393
BpiI GAAGAC 1 cut(s) 1407
BpmI CTGGAG 2 cut(s) 8, 652
BpuEI CTTGAG 1 cut(s) 428
BpuMI CCSGG 5 cut(s) 49, 203, 204, 226, 1050
BsaJI CCNNGG 6 cut(s) 81, 102, 202, 369, 571, 1073
BsaWI WCCGGW 1 cut(s) 119
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 245, 954
Bse118I RCCGGY 1 cut(s) 1330
Bse1I ACTGG 4 cut(s) 669, 711, 836, 959
Bse21I CCTNAGG 1 cut(s) 1241
Bse3DI GCAATG 1 cut(s) 653
BseBI CCWGG 2 cut(s) 573, 1074
BseDI CCNNGG 6 cut(s) 81, 102, 202, 369, 571, 1073
BseGI GGATG 1 cut(s) 1069
BseLI CCNNNNNNNGG 2 cut(s) 245, 954
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 1 cut(s) 1232
BseNI ACTGG 4 cut(s) 669, 711, 836, 959
BseX3I CGGCCG 2 cut(s) 372, 765
BseXI GCAGC 4 cut(s) 334, 935, 1195, 1377
BsgI GTGCAG 1 cut(s) 1202
Bsh1236I CGCG 2 cut(s) 54, 561
Bsh1285I CGRYCG 2 cut(s) 375, 768
BshFI GGCC 4 cut(s) 180, 368, 374, 767
BsiEI CGRYCG 2 cut(s) 375, 768
BsiHKAI GWGCWC 3 cut(s) 549, 668, 933
BsiHKCI CYCGRG 2 cut(s) 202, 548
BsiSI CCGG 7 cut(s) 49, 120, 203, 226, 764, 1049, 1331
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 245, 954
BsmFI GGGAC 1 cut(s) 515
BsmI GAATGC 1 cut(s) 1219
BsnI GGCC 4 cut(s) 180, 368, 374, 767
BsoBI CYCGRG 2 cut(s) 202, 548
Bsp1286I GDGCHC 4 cut(s) 549, 668, 933, 1131
Bsp143I GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
BspACI CCGC 3 cut(s) 64, 898, 1363
BspANI GGCC 4 cut(s) 180, 368, 374, 767
BspCNI CTCAG 1 cut(s) 1233
BspFNI CGCG 2 cut(s) 54, 561
BspLI GGNNCC 5 cut(s) 201, 615, 816, 959, 1264
BspMAI CTGCAG 1 cut(s) 509
BspPI GGATC 5 cut(s) 22, 46, 370, 519, 1254
BspQI GCTCTTC 1 cut(s) 656
BsrDI GCAATG 1 cut(s) 653
BsrFI RCCGGY 1 cut(s) 1330
BsrI ACTGG 4 cut(s) 669, 711, 836, 959
BssAI RCCGGY 1 cut(s) 1330
BssECI CCNNGG 6 cut(s) 81, 102, 202, 369, 571, 1073
BssMI GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 1 cut(s) 102
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 573, 1074
Bst4CI ACNGT 5 cut(s) 164, 292, 335, 456, 1180
Bst6I CTCTTC 2 cut(s) 656, 716
BstC8I GCNNGC 5 cut(s) 272, 320, 1080, 1188, 1219
BstDEI CTNAG 2 cut(s) 566, 1241
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 1 cut(s) 1069
BstFNI CGCG 2 cut(s) 54, 561
BstHHI GCGC 3 cut(s) 54, 450, 561
BstKTI GATC 6 cut(s) 30, 41, 365, 514, 804, 1249
BstMBI GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
BstMCI CGRYCG 2 cut(s) 375, 768
BstMWI GCNNNNNNNGC 4 cut(s) 271, 380, 567, 1365
BstNI CCWGG 2 cut(s) 573, 1074
BstNSI RCATGY 2 cut(s) 683, 1190
BstSCI CCNGG 7 cut(s) 47, 201, 202, 224, 571, 1048, 1072
BstSFI CTRYAG 1 cut(s) 505
BstUI CGCG 2 cut(s) 54, 561
BstV1I GCAGC 4 cut(s) 334, 935, 1195, 1377
BstV2I GAAGAC 1 cut(s) 1407
BstX2I RGATCY 4 cut(s) 27, 38, 511, 1246
BstYI RGATCY 4 cut(s) 27, 38, 511, 1246
BstZI CGGCCG 2 cut(s) 372, 765
Bsu36I CCTNAGG 1 cut(s) 1241
BsuRI GGCC 4 cut(s) 180, 368, 374, 767
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 1069
BtsI GCAGTG 5 cut(s) 272, 610, 1228, 1294, 1375
Cac8I GCNNGC 5 cut(s) 272, 320, 1080, 1188, 1219
CaiI CAGNNNCTG 1 cut(s) 533
CfoI GCGC 3 cut(s) 54, 450, 561
Cfr10I RCCGGY 1 cut(s) 1330
Cfr13I GGNCC 3 cut(s) 178, 199, 1322
Cfr9I CCCGGG 1 cut(s) 202
Csp6I GTAC 1 cut(s) 249
CspCI CAANNNNNGTGG 2 cut(s) 394, 429
CviAII CATG 5 cut(s) 130, 680, 995, 1187, 1342
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 2 cut(s) 566, 1241
DpnI GATC 6 cut(s) 29, 40, 364, 513, 803, 1248
DpnII GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
DraI TTTAAA 1 cut(s) 1171
DrdI GACNNNNNNGTC 1 cut(s) 1409
DseDI GACNNNNNNGTC 1 cut(s) 1409
EaeI YGGCCR 2 cut(s) 372, 765
EagI CGGCCG 2 cut(s) 372, 765
Eam1104I CTCTTC 2 cut(s) 656, 716
EarI CTCTTC 2 cut(s) 656, 716
Ecl136II GAGCTC 1 cut(s) 666
EclXI CGGCCG 2 cut(s) 372, 765
Eco130I CCWWGG 1 cut(s) 102
Eco24I GRGCYC 2 cut(s) 668, 1131
Eco47I GGWCC 2 cut(s) 199, 1322
Eco52I CGGCCG 2 cut(s) 372, 765
Eco53kI GAGCTC 1 cut(s) 666
Eco57I CTGAAG 2 cut(s) 680, 1226
Eco81I CCTNAGG 1 cut(s) 1241
Eco88I CYCGRG 2 cut(s) 202, 548
EcoICRI GAGCTC 1 cut(s) 666
EcoRII CCWGG 2 cut(s) 571, 1072
EcoT14I CCWWGG 1 cut(s) 102
EcoT38I GRGCYC 2 cut(s) 668, 1131
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 5 cut(s) 133, 683, 998, 1190, 1345
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FaqI GGGAC 1 cut(s) 515
FatI CATG 5 cut(s) 129, 679, 994, 1186, 1341
FauNDI CATATG 1 cut(s) 1268
FblI GTMKAC 2 cut(s) 1278, 1335
Fnu4HI GCNGC 4 cut(s) 323, 924, 1184, 1366
FokI GGATG 1 cut(s) 1056
FriOI GRGCYC 2 cut(s) 668, 1131
Fsp4HI GCNGC 4 cut(s) 323, 924, 1184, 1366
FspBI CTAG 3 cut(s) 44, 515, 1302
GlaI GCGC 3 cut(s) 53, 449, 560
GluI GCNGC 4 cut(s) 323, 924, 1184, 1366
GsuI CTGGAG 2 cut(s) 8, 652
HaeIII GGCC 4 cut(s) 180, 368, 374, 767
HapII CCGG 7 cut(s) 49, 120, 203, 226, 764, 1049, 1331
HhaI GCGC 3 cut(s) 54, 450, 561
Hin1II CATG 5 cut(s) 133, 683, 998, 1190, 1345
Hin6I GCGC 3 cut(s) 52, 448, 559
HinP1I GCGC 3 cut(s) 52, 448, 559
HincII GTYRAC 4 cut(s) 193, 1009, 1108, 1279
HindII GTYRAC 4 cut(s) 193, 1009, 1108, 1279
HinfI GANTC 4 cut(s) 235, 257, 432, 1053
HpaI GTTAAC 1 cut(s) 1108
HpaII CCGG 7 cut(s) 49, 120, 203, 226, 764, 1049, 1331
HphI GGTGA 3 cut(s) 347, 368, 1048
Hpy166II GTNNAC 8 cut(s) 109, 151, 193, 283, 1009, 1108, 1279, 1336
Hpy188I TCNGA 7 cut(s) 172, 222, 234, 262, 305, 618, 975
Hpy188III TCNNGA 8 cut(s) 16, 25, 515, 550, 718, 805, 1240, 1354
Hpy8I GTNNAC 8 cut(s) 109, 151, 193, 283, 1009, 1108, 1279, 1336
Hpy99I CGWCG 2 cut(s) 1283, 1415
HpyAV CCTTC 8 cut(s) 41, 353, 770, 820, 975, 1069, 1139, 1313
HpyCH4III ACNGT 5 cut(s) 164, 292, 335, 456, 1180
HpyCH4V TGCA 9 cut(s) 270, 388, 507, 679, 1124, 1183, 1217, 1341, 1384
HpyF10VI GCNNNNNNNGC 4 cut(s) 271, 380, 567, 1365
HpyF3I CTNAG 2 cut(s) 566, 1241
Hsp92II CATG 5 cut(s) 133, 683, 998, 1190, 1345
HspAI GCGC 3 cut(s) 52, 448, 559
KspAI GTTAAC 1 cut(s) 1108
Kzo9I GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 5 cut(s) 262, 619, 671, 814, 1262
Lsp1109I GCAGC 4 cut(s) 334, 935, 1195, 1377
LweI GCATC 2 cut(s) 1111, 1393
MaeI CTAG 3 cut(s) 44, 515, 1302
MaeIII GTNAC 2 cut(s) 182, 725
MalI GATC 6 cut(s) 29, 40, 364, 513, 803, 1248
MboI GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
MboII GAAGA 9 cut(s) 427, 642, 673, 733, 862, 1219, 1283, 1342, 1412
MflI RGATCY 4 cut(s) 27, 38, 511, 1246
MhlI GDGCHC 4 cut(s) 549, 668, 933, 1131
MluCI AATT 9 cut(s) 96, 173, 579, 645, 685, 697, 871, 1086, 1229
MroXI GAANNNNTTC 1 cut(s) 137
MseI TTAA 4 cut(s) 684, 1089, 1107, 1170
MslI CAYNNNNRTG 2 cut(s) 134, 1022
MspI CCGG 7 cut(s) 49, 120, 203, 226, 764, 1049, 1331
MspR9I CCNGG 7 cut(s) 49, 203, 204, 226, 573, 1050, 1074
Mva1269I GAATGC 1 cut(s) 1219
MvaI CCWGG 2 cut(s) 573, 1074
MvnI CGCG 2 cut(s) 54, 561
MwoI GCNNNNNNNGC 4 cut(s) 271, 380, 567, 1365
NciI CCSGG 5 cut(s) 49, 203, 204, 226, 1050
NdeI CATATG 1 cut(s) 1268
NdeII GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
NlaIII CATG 5 cut(s) 133, 683, 998, 1190, 1345
NlaIV GGNNCC 5 cut(s) 201, 615, 816, 959, 1264
NmeAIII GCCGAG 2 cut(s) 350, 1216
NmuCI GTSAC 1 cut(s) 182
NspI RCATGY 2 cut(s) 683, 1190
OliI CACNNNNGTG 1 cut(s) 1022
PaeI GCATGC 1 cut(s) 1190
PaeR7I CTCGAG 1 cut(s) 548
PciSI GCTCTTC 1 cut(s) 656
PctI GAATGC 1 cut(s) 1219
PdmI GAANNNNTTC 1 cut(s) 137
PfeI GAWTC 4 cut(s) 235, 257, 432, 1053
PflFI GACNNNGTC 1 cut(s) 188
PflMI CCANNNNNTGG 1 cut(s) 954
PkrI GCNGC 4 cut(s) 324, 925, 1185, 1367
Psp124BI GAGCTC 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 571, 1072
PspGI CCWGG 2 cut(s) 571, 1072
PspN4I GGNNCC 5 cut(s) 201, 615, 816, 959, 1264
PspPI GGNCC 3 cut(s) 178, 199, 1322
PstI CTGCAG 1 cut(s) 509
PstNI CAGNNNCTG 1 cut(s) 533
PsuI RGATCY 4 cut(s) 27, 38, 511, 1246
PsyI GACNNNGTC 1 cut(s) 188
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
RseI CAYNNNNRTG 2 cut(s) 134, 1022
SacI GAGCTC 1 cut(s) 668
SalI GTCGAC 1 cut(s) 1277
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 4 cut(s) 684, 1089, 1107, 1170
SatI GCNGC 4 cut(s) 323, 924, 1184, 1366
Sau3AI GATC 6 cut(s) 27, 38, 362, 511, 801, 1246
Sau96I GGNCC 3 cut(s) 178, 199, 1322
ScrFI CCNGG 7 cut(s) 49, 203, 204, 226, 573, 1050, 1074
SduI GDGCHC 4 cut(s) 549, 668, 933, 1131
SfaNI GCATC 2 cut(s) 1111, 1393
SfcI CTRYAG 1 cut(s) 505
Sfr274I CTCGAG 1 cut(s) 548
SinI GGWCC 2 cut(s) 199, 1322
SlaI CTCGAG 1 cut(s) 548
SmaI CCCGGG 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 134, 1022
SmlI CTYRAG 3 cut(s) 14, 443, 548
SmoI CTYRAG 3 cut(s) 14, 443, 548
SphI GCATGC 1 cut(s) 1190
Sse9I AATT 9 cut(s) 96, 173, 579, 645, 685, 697, 871, 1086, 1229
SsiI CCGC 3 cut(s) 64, 898, 1363
SspMI CTAG 3 cut(s) 44, 515, 1302
SstI GAGCTC 1 cut(s) 668
StyD4I CCNGG 7 cut(s) 47, 201, 202, 224, 571, 1048, 1072
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 5 cut(s) 164, 292, 335, 456, 1180
TaqI TCGA 8 cut(s) 255, 492, 549, 804, 912, 1113, 1278, 1413
TasI AATT 9 cut(s) 96, 173, 579, 645, 685, 697, 871, 1086, 1229
TfiI GAWTC 4 cut(s) 235, 257, 432, 1053
Tru1I TTAA 4 cut(s) 684, 1089, 1107, 1170
Tru9I TTAA 4 cut(s) 684, 1089, 1107, 1170
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 4 cut(s) 322, 923, 1183, 1365
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 6 cut(s) 146, 202, 438, 642, 1158, 1226
TspGWI ACGGA 2 cut(s) 51, 173
TspMI CCCGGG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 188
Van91I CCANNNNNTGG 1 cut(s) 954
VpaK11BI GGWCC 2 cut(s) 199, 1322
XbaI TCTAGA 1 cut(s) 514
XceI RCATGY 2 cut(s) 683, 1190
XhoI CTCGAG 1 cut(s) 548
XmaI CCCGGG 1 cut(s) 202
XmiI GTMKAC 2 cut(s) 1278, 1335
XmnI GAANNNNTTC 1 cut(s) 137
XspI CTAG 3 cut(s) 44, 515, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.