FvH4_6g30120

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005191
Physical Location & Seq
Forward (+)
23274097 .. 23279638
5542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30120.t2

Sequence Viewer

Length: 3468 bp
ATGGCTGAAGCAATCATCATCACCCTTGCAGCTCCTGTTGTGAAGATTGCCCTCACTAAATTGACTTCTCTTGTTACCGAGGAGTTTCTTGCATTCACCAATGTCAGGGATGACATGGATACCCTCTGCAGCACTTTGACAGATATTTGTCAGTTGCTTGAAGTTTTGGAAAGAAATCGGACGAATTCTTCTGATGGTTTCCACCAGATGAACAATTGGCTCAAGAAGCTTCAAATAGCAGGTTATGATGCAGGCGATTTAATCGAGTCCTGGGCAGCAGAATATCACCAGTGGAAGACGAAGAAGCAGGTACAAAAACTCTCTCTTCCATTTAGTGCTTCAAAATTTTCCTTTCAGCGCCAAGAAGCATGCGACTTGAAAGAAATTACAGCGAGAATAAAAAAGATTTTACAAGAAGGACAAGTCTATAGAACCATCATTGGAAATCCTCCAATGCGGACTGAAAGCCTTTCACGGGAAACTGGCTCTCTTTCTAACAACATAGTCGAGGGAAGGGGAGACGATGTAGAGAACATAATAAAACTGCTGATACCAGATGAAGAAGCCAGTAATGGAGGCGATATTGATTTTATTCCAATTGTGGGGATGGGCGGCTTGGGTAAAACAACTCTTGCTCAAGTCGTATATAATGATGCAAGAGTTCATGATCATTTTAAAAGAAAATTCTGGGTTTGTGTTACAGAAAATTATGACGAGAAGAAAATTCTCAAAGGAATTTTGGAGTCTAATAAGAGTATTTTTGATGCCGACATCTCATTAGTGGTGCTGCAAGACCGAGTTCGTGAGCTCCTGGTTGAAAAACCATTCTTACTTGTTCTAGATGATTTATGGAACGATGATTATATGAAATTGCAGCCACTAGAAAATTTACTGAAACAAGGCGTCCGTGGAAGTAAGGTCTTGGTTACTACTCGGAACAATGAAGTGTGGAAGATTACAGGTGCAAAAGCCTCCTATAGCTTGCGAGCTTTTAATGATAATGAATCATTGGCGTTATTGGAGAAAATTGCCTTTAAAGAAAGTGCTTTGCCAGATGAGTTGAAGGAATATGCAAAGCAAATTGTAAACAGGTGCAACGGTTTACCTCTGGCAGTAAAACAAGTTGGGGGTCTGCTCCGTGGAATCACTGAGGCAGAGGAGTGGAGTTATATCGCTAAAAGTGAAATATGGGAACTAAAAAAAGATAAAGTACTGCCTGCTCTTCGATTGAGTTACAACAATCTTTCTTCAGCTCTAAAGCAGTGTTTTGCATTTTGTTCCCTGTTTCCGAAAGCTCATGTTTTTGACAAAACTGAGTTGGTAAAATTATGGACAGCAGAAGCATTCATTCAACCTCATGAAAGACACAGGACAGAAGTCATAGGAAGCAGATACTTCAAAGAGCTGTCAGACAGGTTCTTCTTTGAGATCTTGGCGGAAGACGAGAACAAGGGCAGAATGCATGATCTTGTCCATGACTTGGCGCTATCCATCTCCTCTCCCTTTTGCTGCCAAGTGAAAGATGTTGTGCCAAACAAATTTGAGGAAGTTACTCGCCATGTATCACTGCTTTCAAACGAGGTTGAGCAGCCCCTTTCAGAAATAATCAAAAGATCAAAGAAGCTGCGCACGCTTTTATTGCCAAATAAACAGTTCAAAGCCTTTGGGAAAGCTGACCGTGAAATATTCCATTCACTCAAGTACATGCGCACGCTAGACTTGAGTTCAAGCACACTTCAAGAGCTGTCAGGCTCAATCGAGAATTTGAAGCTTTTGCACTACCTTGATTTATCAAAAACTGAAATTAAAAAGCTTCCTGATTCAATATGCAACCTCTACCAATTGGAGACGTTGAAACTTTCAGATTGCCCTTGGCTTTTCACTTTGCCCAGGAAGCTCAAAACTCTTGTCAACCTGAGACATCTGGAGCTAGATGAAATGTTTTGGTACACAGTATCGACGCTTCCAGAAAGCATTGGGTGTCTAACCGGTCTGCACAATCTTCACAAATTTCAGGTTGGCTGCAATACAGGATACAAACTTCAAGAATTGAAGAAAATGGAGTACCTTACAGGGTTCCTGCACATCTCAAACCTGGAAAACGCAGTAGATGCACGGGAGGCCAACTTGAAAGAAAAAGAAATGATTAAAAAACTGGTATATGAATGGAGCAGCAGCAATCTGAACTTGCAAGATGAAGACGCCAAGCAGGTACTCGAAGACCTGCAGCCTCACCCAATGGTCCAAGACATCCAAATCTGTCACTACAGGAGCTCTGAATTTCCAATTTGGATCAGATACGGAAAGTATGAAAATTTGGGTTCCATATACTTAAATCATTGCACAGGGATCAAAATCCTCTCTCTTGGCGAGTTACCCAACCTCAGAGAACTCCGTCTCAAAAACATGCCAGAAATGATGGAATGGAAGGAAGAGAGAATCCTTTTCTTCTCGCGTCTACATATAAGTGGCTGCCCAAAGCTGACAAAACTGCCCAGCATGTTTTCTGCATTACGCAGTATGAAGATCAAGAATTGTGAATCATTGGATACCATTCCAGTTGGCCCTGTGCAATTCATAACACTTGCTGGCAATCCTGTACTGAAGCACTGGACTGAAGCCGATGTATTTGCAAGGAGCGTTGTCGATGGTCGCCCAGTCATAAGTCATGGCATGACAAGTCATTCTCTGTTGGAAGCAAACATTATCAACTGCCCTGAACTGCAGACGTTGCCAAGCGAACTTTATCCACAGAAACTGGAAATCAGCAGGTGCAAGAAACTGCGCACCCTACCAGATGCAAACCATGCACAACGGCTTCAGGTTTTAGCATTGGATGCATGTCATGATGAAACTTTAGTCGGTATGATCCCAAGTTCTGAGACTTTATATTCCTTGGTGATATCTAACATCCCAAATCTCATTTGTCTCCCAAAGTGGCCTAGTCTCCCTGGCCTCCAAGCATTGTATATCCGTGATTGCGCAGACCTTGCGTATCTATCCAACCAAGAGAACGGGAACTGGTTGTTTGAGTGCTTCAACTCTCTAAAGCATCTCTCCATCCGTAACTGTCCTAAACTTCTGACGCTGCCAGCTGAAGGCCTCCCAACCAGCCTACAAGTTTTGAGTATTGGCCCATGTGCAAGTCTCAGTTCTTTCGGCCCCGGGGAAGTATTCGCAAAACTCACATCCCTTACTGATCTTTACCTTGAAGATTGCCCAGCACTCGAGTCCTTGCCTAAGGAAGGCCTGTCAGCGTCTGTCCAGCATCTGTCTATCCAAGGATGCCCGTCGCTGATAAGAAGTTGTGGAAAAGACTGCAATGATTGGCCAAAGATCAAGGACATCCCGGATCTGGAAATAGAGACGCCATCTACCGAAACTTCAAGCTCATCCTCAGCAAGTTGGTACAATCTTCGTAGGAACCGCAAAGGAAACAAATCTAAAGGAAAGAAGGCTTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1156

Amino Acids

131.07

Weight (kDa)

6.57

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 103 1.5e-12 Rx N-terminal domain
NB-ARC PF00931 191 - 348 1.8e-30 NB-ARC domain
WHD_DRP PF23559 428 - 495 2.3e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 557 - 808 1.6e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 681 - 803 2.4e-27 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2760
Acc16I TGCGCA 4 cut(s) 1628, 1709, 2786, 2983
Acc36I ACCTGC 5 cut(s) 230, 298, 2200, 2232, 2760
AccB7I CCANNNNNTGG 1 cut(s) 1480
AccI GTMKAC 1 cut(s) 2458
AccII CGCG 1 cut(s) 2455
AciI CCGC 4 cut(s) 457, 612, 1436, 3427
AclWI GGATC 4 cut(s) 2300, 2357, 2863, 3360
AcoI YGGCCR 1 cut(s) 3329
AcuI CTGAAG 6 cut(s) 27, 1233, 2624, 2637, 2804, 3117
AcyI GRCGYC 3 cut(s) 903, 2202, 3368
AfaI GTAC 8 cut(s) 312, 1212, 1703, 1949, 2066, 2214, 2601, 3410
AfiI CCNNNNNNNGG 7 cut(s) 105, 475, 602, 1480, 3098, 3245, 3355
AgeI ACCGGT 1 cut(s) 1988
AjnI CCWGG 5 cut(s) 269, 810, 1889, 2094, 2950
Alw21I GWGCWC 2 cut(s) 810, 2276
Alw26I GTCTC 9 cut(s) 513, 1841, 1912, 2402, 2876, 2933, 2951, 3152, 3359
AlwI GGATC 4 cut(s) 2300, 2357, 2863, 3360
AlwNI CAGNNNCTG 4 cut(s) 35, 2758, 3260, 3271
Ama87I CYCGRG 2 cut(s) 3164, 3227
AoxI GGCC 9 cut(s) 2121, 2563, 2939, 2953, 3100, 3133, 3160, 3247, 3329
ArsI GACNNNNNNTTYG 2 cut(s) 1893, 1925
AsiGI ACCGGT 1 cut(s) 1988
AspLEI GCGC 6 cut(s) 360, 1486, 1629, 1710, 2787, 2984
AspS9I GGNCC 4 cut(s) 2242, 2564, 3134, 3161
AsuC2I CCSGG 3 cut(s) 3165, 3166, 3350
AsuHPI GGTGA 5 cut(s) 13, 88, 278, 2225, 2911
AvaI CYCGRG 2 cut(s) 3164, 3227
AvaII GGWCC 1 cut(s) 2242
AxyI CCTNAGG 1 cut(s) 3240
BalI TGGCCA 1 cut(s) 3331
BanII GRGCYC 2 cut(s) 810, 2276
BarI GAAGNNNNNNTAC 2 cut(s) 1947, 1979
BbsI GAAGAC 4 cut(s) 302, 1446, 2205, 2226
Bbv12I GWGCWC 2 cut(s) 810, 2276
BbvCI CCTCAGC 1 cut(s) 3397
BccI CCATC 8 cut(s) 188, 443, 601, 1499, 2413, 2642, 3068, 3379
BceAI ACGGC 1 cut(s) 2831
BcgI CGANNNNNNTGC 2 cut(s) 2612, 2646
BciT130I CCWGG 5 cut(s) 271, 812, 1891, 2096, 2952
BciVI GTATCC 3 cut(s) 112, 2027, 2542
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 3 cut(s) 3165, 3166, 3350
BcoDI GTCTC 9 cut(s) 513, 1841, 1912, 2402, 2876, 2933, 2951, 3152, 3359
BfaI CTAG 5 cut(s) 839, 881, 1715, 1931, 2943
BfmI CTRYAG 6 cut(s) 127, 427, 976, 2225, 2266, 2723
BfoI RGCGCY 2 cut(s) 361, 1487
BfuAI ACCTGC 5 cut(s) 230, 298, 2200, 2232, 2760
BfuI GTATCC 3 cut(s) 112, 2027, 2542
BglII AGATCT 1 cut(s) 1428
BmcAI AGTACT 1 cut(s) 1212
Bme1390I CCNGG 8 cut(s) 271, 812, 1891, 2096, 2952, 3165, 3166, 3350
Bme18I GGWCC 1 cut(s) 2242
BmeT110I CYCGRG 2 cut(s) 3164, 3227
BmgT120I GGNCC 4 cut(s) 2242, 2564, 3134, 3161
BmiI GGNNCC 4 cut(s) 2078, 2323, 3163, 3425
BmrFI CCNGG 8 cut(s) 271, 812, 1891, 2096, 2952, 3165, 3166, 3350
BmrI ACTGGG 1 cut(s) 2651
BmsI GCATC 9 cut(s) 238, 643, 754, 2101, 2788, 2827, 3061, 3275, 3277
BmuI ACTGGG 1 cut(s) 2651
BoxI GACNNNNGTC 1 cut(s) 1376
BpiI GAAGAC 4 cut(s) 302, 1446, 2205, 2226
BpmI CTGGAG 1 cut(s) 1946
Bpu10I CCTNAGC 1 cut(s) 3397
BpuEI CTTGAG 4 cut(s) 206, 621, 1682, 1741
BpuMI CCSGG 3 cut(s) 3165, 3166, 3350
BsaBI GATNNNNATC 1 cut(s) 2354
BsaHI GRCGYC 3 cut(s) 903, 2202, 3368
BsaWI WCCGGW 1 cut(s) 1988
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 7 cut(s) 105, 475, 602, 1480, 3098, 3245, 3355
Bse118I RCCGGY 1 cut(s) 1988
Bse1I ACTGG 9 cut(s) 289, 487, 567, 2160, 2558, 2615, 2657, 2763, 3026
Bse21I CCTNAGG 1 cut(s) 3240
Bse3DI GCAATG 2 cut(s) 2338, 3328
Bse8I GATNNNNATC 1 cut(s) 2354
BseBI CCWGG 5 cut(s) 271, 812, 1891, 2096, 2952
BseJI GATNNNNATC 1 cut(s) 2354
BseLI CCNNNNNNNGG 7 cut(s) 105, 475, 602, 1480, 3098, 3245, 3355
BseMI GCAATG 2 cut(s) 2338, 3328
BseMII CTCAG 7 cut(s) 1140, 1305, 1907, 2398, 2871, 3163, 3411
BseNI ACTGG 9 cut(s) 289, 487, 567, 2160, 2558, 2615, 2657, 2763, 3026
BseRI GAGGAG 3 cut(s) 95, 1172, 1486
BseYI CCCAGC 2 cut(s) 2495, 3220
BsgI GTGCAG 2 cut(s) 1979, 2066
Bsh1236I CGCG 1 cut(s) 2455
BshFI GGCC 9 cut(s) 2123, 2565, 2941, 2955, 3102, 3135, 3162, 3249, 3331
BshTI ACCGGT 1 cut(s) 1988
BsiHKAI GWGCWC 2 cut(s) 810, 2276
BsiHKCI CYCGRG 2 cut(s) 3164, 3227
BsiSI CCGG 3 cut(s) 1989, 3165, 3350
BslI CCNNNNNNNGG 7 cut(s) 105, 475, 602, 1480, 3098, 3245, 3355
BsmAI GTCTC 9 cut(s) 513, 1841, 1912, 2402, 2876, 2933, 2951, 3152, 3359
BsmBI CGTCTC 4 cut(s) 513, 1841, 2402, 3359
BsmI GAATGC 3 cut(s) 92, 1343, 1464
BsnI GGCC 9 cut(s) 2123, 2565, 2941, 2955, 3102, 3135, 3162, 3249, 3331
BsoBI CYCGRG 2 cut(s) 3164, 3227
Bsp1286I GDGCHC 2 cut(s) 810, 2276
BspACI CCGC 4 cut(s) 457, 612, 1436, 3427
BspANI GGCC 9 cut(s) 2123, 2565, 2941, 2955, 3102, 3135, 3162, 3249, 3331
BspCNI CTCAG 7 cut(s) 1141, 1306, 1908, 2397, 2872, 3162, 3410
BspFNI CGCG 1 cut(s) 2455
BspHI TCATGA 3 cut(s) 664, 1357, 2845
BspLI GGNNCC 4 cut(s) 2078, 2323, 3163, 3425
BspMAI CTGCAG 3 cut(s) 131, 2229, 2727
BspMI ACCTGC 5 cut(s) 230, 298, 2200, 2232, 2760
BspPI GGATC 4 cut(s) 2300, 2357, 2863, 3360
BspQI GCTCTTC 1 cut(s) 1227
BsrDI GCAATG 2 cut(s) 2338, 3328
BsrFI RCCGGY 1 cut(s) 1988
BsrI ACTGG 9 cut(s) 289, 487, 567, 2160, 2558, 2615, 2657, 2763, 3026
BssAI RCCGGY 1 cut(s) 1988
BssNI GRCGYC 3 cut(s) 903, 2202, 3368
BssT1I CCWWGG 3 cut(s) 1871, 2895, 3280
Bst2UI CCWGG 5 cut(s) 271, 812, 1891, 2096, 2952
Bst4CI ACNGT 5 cut(s) 1100, 1653, 1679, 1954, 3071
Bst6I CTCTTC 3 cut(s) 330, 1227, 2427
BstACI GRCGYC 3 cut(s) 903, 2202, 3368
BstAPI GCANNNNNTGC 5 cut(s) 2111, 2731, 2807, 2837, 2990
BstC8I GCNNGC 9 cut(s) 253, 370, 983, 987, 1218, 1631, 1712, 2590, 3093
BstDEI CTNAG 8 cut(s) 1149, 1314, 1916, 2384, 2880, 3149, 3240, 3397
BstDSI CCRYGG 2 cut(s) 907, 1138
BstENI CCTNNNNNAGG 1 cut(s) 3243
BstFNI CGCG 1 cut(s) 2455
BstH2I RGCGCY 2 cut(s) 361, 1487
BstHHI GCGC 6 cut(s) 360, 1486, 1629, 1710, 2787, 2984
BstMAI GTCTC 9 cut(s) 513, 1841, 1912, 2402, 2876, 2933, 2951, 3152, 3359
BstNI CCWGG 5 cut(s) 271, 812, 1891, 2096, 2952
BstNSI RCATGY 5 cut(s) 372, 1708, 2410, 2503, 2844
BstPAI GACNNNNGTC 1 cut(s) 1376
BstSCI CCNGG 8 cut(s) 269, 810, 1889, 2094, 2950, 3163, 3164, 3348
BstSFI CTRYAG 6 cut(s) 127, 427, 976, 2225, 2266, 2723
BstUI CGCG 1 cut(s) 2455
BstV2I GAAGAC 4 cut(s) 302, 1446, 2205, 2226
BstX2I RGATCY 2 cut(s) 1428, 3352
BstYI RGATCY 2 cut(s) 1428, 3352
Bsu36I CCTNAGG 1 cut(s) 3240
BsuI GTATCC 3 cut(s) 112, 2027, 2542
BsuRI GGCC 9 cut(s) 2123, 2565, 2941, 2955, 3102, 3135, 3162, 3249, 3331
BtgI CCRYGG 2 cut(s) 907, 1138
BtsI GCAGTG 2 cut(s) 1268, 1565
BtsIMutI CAGTG 5 cut(s) 296, 1146, 1268, 1565, 2608
BveI ACCTGC 5 cut(s) 230, 298, 2200, 2232, 2760
Cac8I GCNNGC 9 cut(s) 253, 370, 983, 987, 1218, 1631, 1712, 2590, 3093
CaiI CAGNNNCTG 4 cut(s) 35, 2758, 3260, 3271
CciI TCATGA 3 cut(s) 664, 1357, 2845
CfoI GCGC 6 cut(s) 360, 1486, 1629, 1710, 2787, 2984
Cfr10I RCCGGY 1 cut(s) 1988
Cfr13I GGNCC 4 cut(s) 2242, 2564, 3134, 3161
Cfr9I CCCGGG 1 cut(s) 3164
CseI GACGC 7 cut(s) 892, 1969, 2210, 2444, 3094, 3246, 3376
Csp6I GTAC 8 cut(s) 311, 1211, 1702, 1948, 2065, 2213, 2600, 3409
CspAI ACCGGT 1 cut(s) 1988
CviQI GTAC 8 cut(s) 311, 1211, 1702, 1948, 2065, 2213, 2600, 3409
DdeI CTNAG 8 cut(s) 1149, 1314, 1916, 2384, 2880, 3149, 3240, 3397
DraI TTTAAA 2 cut(s) 676, 1036
EaeI YGGCCR 1 cut(s) 3329
Eam1104I CTCTTC 3 cut(s) 330, 1227, 2427
EarI CTCTTC 3 cut(s) 330, 1227, 2427
EciI GGCGGA 1 cut(s) 1451
Ecl136II GAGCTC 2 cut(s) 808, 2274
Eco130I CCWWGG 3 cut(s) 1871, 2895, 3280
Eco147I AGGCCT 2 cut(s) 3102, 3249
Eco24I GRGCYC 2 cut(s) 810, 2276
Eco32I GATATC 1 cut(s) 2904
Eco47I GGWCC 1 cut(s) 2242
Eco53kI GAGCTC 2 cut(s) 808, 2274
Eco57I CTGAAG 6 cut(s) 27, 1233, 2624, 2637, 2804, 3117
Eco81I CCTNAGG 1 cut(s) 3240
Eco88I CYCGRG 2 cut(s) 3164, 3227
EcoICRI GAGCTC 2 cut(s) 808, 2274
EcoNI CCTNNNNNAGG 1 cut(s) 3243
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 5 cut(s) 269, 810, 1889, 2094, 2950
EcoRV GATATC 1 cut(s) 2904
EcoT14I CCWWGG 3 cut(s) 1871, 2895, 3280
EcoT22I ATGCAT 2 cut(s) 1464, 2842
EcoT38I GRGCYC 2 cut(s) 810, 2276
ErhI CCWWGG 3 cut(s) 1871, 2895, 3280
Esp3I CGTCTC 4 cut(s) 513, 1841, 2402, 3359
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 1 cut(s) 2458
FriOI GRGCYC 2 cut(s) 810, 2276
FspAI RTGCGCAY 1 cut(s) 1709
FspBI CTAG 5 cut(s) 839, 881, 1715, 1931, 2943
FspI TGCGCA 4 cut(s) 1628, 1709, 2786, 2983
GlaI GCGC 6 cut(s) 359, 1485, 1628, 1709, 2786, 2983
GsaI CCCAGC 2 cut(s) 2499, 3224
GsuI CTGGAG 1 cut(s) 1946
HaeII RGCGCY 2 cut(s) 361, 1487
HaeIII GGCC 9 cut(s) 2123, 2565, 2941, 2955, 3102, 3135, 3162, 3249, 3331
HapII CCGG 3 cut(s) 1989, 3165, 3350
HgaI GACGC 7 cut(s) 892, 1969, 2210, 2444, 3094, 3246, 3376
HhaI GCGC 6 cut(s) 360, 1486, 1629, 1710, 2787, 2984
Hin1I GRCGYC 3 cut(s) 903, 2202, 3368
Hin6I GCGC 6 cut(s) 358, 1484, 1627, 1708, 2785, 2982
HinP1I GCGC 6 cut(s) 358, 1484, 1627, 1708, 2785, 2982
HincII GTYRAC 1 cut(s) 1912
HindII GTYRAC 1 cut(s) 1912
HindIII AAGCTT 3 cut(s) 227, 1769, 1811
HinfI GANTC 8 cut(s) 266, 743, 1004, 1143, 1820, 2439, 2540, 3230
HpaII CCGG 3 cut(s) 1989, 3165, 3350
HphI GGTGA 5 cut(s) 13, 88, 278, 2225, 2911
Hpy166II GTNNAC 5 cut(s) 1087, 1103, 1912, 1950, 2459
Hpy8I GTNNAC 5 cut(s) 1087, 1103, 1912, 1950, 2459
Hpy99I CGWCG 2 cut(s) 1963, 3295
HpyAV CCTTC 7 cut(s) 410, 507, 1057, 2422, 3092, 3239, 3448
HpyCH4III ACNGT 5 cut(s) 1100, 1653, 1679, 1954, 3071
HpyCH4IV ACGT 2 cut(s) 1850, 2729
HpyF3I CTNAG 8 cut(s) 1149, 1314, 1916, 2384, 2880, 3149, 3240, 3397
HpySE526I ACGT 2 cut(s) 1850, 2729
Hsp92I GRCGYC 3 cut(s) 903, 2202, 3368
HspAI GCGC 6 cut(s) 358, 1484, 1627, 1708, 2785, 2982
Ksp22I TGATCA 1 cut(s) 667
LguI GCTCTTC 1 cut(s) 1227
LmnI GCTCC 7 cut(s) 37, 813, 1140, 1927, 2169, 2271, 2637
LweI GCATC 9 cut(s) 238, 643, 754, 2101, 2788, 2827, 3061, 3275, 3277
MaeI CTAG 5 cut(s) 839, 881, 1715, 1931, 2943
MaeII ACGT 2 cut(s) 1850, 2729
MaeIII GTNAC 8 cut(s) 73, 697, 925, 1232, 1549, 2261, 2373, 3065
MfeI CAATTG 3 cut(s) 214, 597, 1841
MflI RGATCY 2 cut(s) 1428, 3352
MhlI GDGCHC 2 cut(s) 810, 2276
MlsI TGGCCA 1 cut(s) 3331
MluNI TGGCCA 1 cut(s) 3331
MlyI GAGTC 3 cut(s) 275, 752, 3239
MmeI TCCRAC 2 cut(s) 2673, 3027
Mox20I TGGCCA 1 cut(s) 3331
Mph1103I ATGCAT 2 cut(s) 1464, 2842
MscI TGGCCA 1 cut(s) 3331
MseI TTAA 7 cut(s) 260, 675, 993, 1035, 1806, 2148, 2333
MslI CAYNNNNRTG 1 cut(s) 2466
Msp20I TGGCCA 1 cut(s) 3331
MspA1I CMGCKG 1 cut(s) 3095
MspI CCGG 3 cut(s) 1989, 3165, 3350
MspR9I CCNGG 8 cut(s) 271, 812, 1891, 2096, 2952, 3165, 3166, 3350
MunI CAATTG 3 cut(s) 214, 597, 1841
Mva1269I GAATGC 3 cut(s) 92, 1343, 1464
MvaI CCWGG 5 cut(s) 271, 812, 1891, 2096, 2952
MvnI CGCG 1 cut(s) 2455
NciI CCSGG 3 cut(s) 3165, 3166, 3350
NlaIV GGNNCC 4 cut(s) 2078, 2323, 3163, 3425
NmuCI GTSAC 1 cut(s) 2261
NsbI TGCGCA 4 cut(s) 1628, 1709, 2786, 2983
NsiI ATGCAT 2 cut(s) 1464, 2842
NspI RCATGY 5 cut(s) 372, 1708, 2410, 2503, 2844
PaeI GCATGC 1 cut(s) 372
PaeR7I CTCGAG 1 cut(s) 3227
PagI TCATGA 3 cut(s) 664, 1357, 2845
PaqCI CACCTGC 1 cut(s) 2760
PceI AGGCCT 2 cut(s) 3102, 3249
PciSI GCTCTTC 1 cut(s) 1227
PctI GAATGC 3 cut(s) 92, 1343, 1464
PfeI GAWTC 5 cut(s) 1004, 1143, 1820, 2439, 2540
PflMI CCANNNNNTGG 1 cut(s) 1480
PfoI TCCNGGA 1 cut(s) 3348
PinAI ACCGGT 1 cut(s) 1988
PleI GAGTC 3 cut(s) 274, 751, 3238
PpsI GAGTC 3 cut(s) 274, 751, 3238
PshAI GACNNNNGTC 1 cut(s) 1376
Psp124BI GAGCTC 2 cut(s) 810, 2276
Psp6I CCWGG 5 cut(s) 269, 810, 1889, 2094, 2950
PspFI CCCAGC 2 cut(s) 2495, 3220
PspGI CCWGG 5 cut(s) 269, 810, 1889, 2094, 2950
PspN4I GGNNCC 4 cut(s) 2078, 2323, 3163, 3425
PspPI GGNCC 4 cut(s) 2242, 2564, 3134, 3161
PspXI VCTCGAGB 1 cut(s) 3227
PstI CTGCAG 3 cut(s) 131, 2229, 2727
PstNI CAGNNNCTG 4 cut(s) 35, 2758, 3260, 3271
PsuI RGATCY 2 cut(s) 1428, 3352
PvuII CAGCTG 1 cut(s) 3095
RsaI GTAC 8 cut(s) 312, 1212, 1703, 1949, 2066, 2214, 2601, 3410
RsaNI GTAC 8 cut(s) 311, 1211, 1702, 1948, 2065, 2213, 2600, 3409
RseI CAYNNNNRTG 1 cut(s) 2466
SacI GAGCTC 2 cut(s) 810, 2276
SapI GCTCTTC 1 cut(s) 1227
SaqAI TTAA 7 cut(s) 260, 675, 993, 1035, 1806, 2148, 2333
Sau96I GGNCC 4 cut(s) 2242, 2564, 3134, 3161
ScaI AGTACT 1 cut(s) 1212
SchI GAGTC 3 cut(s) 275, 752, 3239
ScrFI CCNGG 8 cut(s) 271, 812, 1891, 2096, 2952, 3165, 3166, 3350
SduI GDGCHC 2 cut(s) 810, 2276
SfaNI GCATC 9 cut(s) 238, 643, 754, 2101, 2788, 2827, 3061, 3275, 3277
SfcI CTRYAG 6 cut(s) 127, 427, 976, 2225, 2266, 2723
Sfr274I CTCGAG 1 cut(s) 3227
SinI GGWCC 1 cut(s) 2242
SlaI CTCGAG 1 cut(s) 3227
SmaI CCCGGG 1 cut(s) 3166
SmiMI CAYNNNNRTG 1 cut(s) 2466
SmlI CTYRAG 5 cut(s) 221, 636, 1697, 1720, 3227
SmoI CTYRAG 5 cut(s) 221, 636, 1697, 1720, 3227
SphI GCATGC 1 cut(s) 372
SseBI AGGCCT 2 cut(s) 3102, 3249
SsiI CCGC 4 cut(s) 457, 612, 1436, 3427
SspI AATATT 1 cut(s) 1686
SspMI CTAG 5 cut(s) 839, 881, 1715, 1931, 2943
SstI GAGCTC 2 cut(s) 810, 2276
StuI AGGCCT 2 cut(s) 3102, 3249
StyD4I CCNGG 8 cut(s) 269, 810, 1889, 2094, 2950, 3163, 3164, 3348
StyI CCWWGG 3 cut(s) 1871, 2895, 3280
TaaI ACNGT 5 cut(s) 1100, 1653, 1679, 1954, 3071
TaiI ACGT 2 cut(s) 1853, 2732
TaqI TCGA 8 cut(s) 264, 507, 1225, 1758, 1958, 2217, 2646, 3228
TaqII GACCGA 1 cut(s) 810
TatI WGTACW 3 cut(s) 1210, 1701, 2599
TauI GCSGC 1 cut(s) 615
TfiI GAWTC 5 cut(s) 1004, 1143, 1820, 2439, 2540
Tru1I TTAA 7 cut(s) 260, 675, 993, 1035, 1806, 2148, 2333
Tru9I TTAA 7 cut(s) 260, 675, 993, 1035, 1806, 2148, 2333
TscAI CASTG 5 cut(s) 296, 1153, 1268, 1572, 2615
TseFI GTSAC 1 cut(s) 2261
Tsp45I GTSAC 1 cut(s) 2261
TspGWI ACGGA 6 cut(s) 896, 1127, 2316, 2384, 2963, 3053
TspMI CCCGGG 1 cut(s) 3164
TspRI CASTG 5 cut(s) 296, 1153, 1268, 1572, 2615
Van91I CCANNNNNTGG 1 cut(s) 1480
VpaK11BI GGWCC 1 cut(s) 2242
XagI CCTNNNNNAGG 1 cut(s) 3243
XbaI TCTAGA 1 cut(s) 838
XceI RCATGY 5 cut(s) 372, 1708, 2410, 2503, 2844
XhoI CTCGAG 1 cut(s) 3227
XmaI CCCGGG 1 cut(s) 3164
XmiI GTMKAC 1 cut(s) 2458
XspI CTAG 5 cut(s) 839, 881, 1715, 1931, 2943
ZrmI AGTACT 1 cut(s) 1212
Zsp2I ATGCAT 2 cut(s) 1464, 2842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.