MD11G1079400.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005191
Physical Location & Seq
Reverse (-)
6810609 .. 6815218
4610 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1079400.v1.1.491

Sequence Viewer

Length: 3816 bp
ATGGCTGACTTACTTATCAGCCTTGCAAGTCCTATTGTAGAGATTGCAACGAAGAAACTGGCTAATGTTGCGACTAATGAATTCGTAAATATCGAGGATGAGGTAGAGAGGCTGAAACTTTCTTTTATGGAAGTTCAAGATTTGCTTAAAATTTTGGAAGGAAATCATCAGATGAATTCTTCTACTCCTTCCACCCTGAAGAATTGGTTCGAAAGGCTACGAGCAGCATCTTATGATATTGAGGATTTGATCGACTGTTGGGCAGCAGAATGTGAATGGTGGAAGATGAAGAAGCAGCAGCAGCAGCAGGTACACAAATTCCATCTTCTGTTTGGTGCTTTTAAGTTTCTCTTTCTGCATCGTTTATTGTCTAAGTTGAGAGAAATTACATCAAGACTACATGTGATTAAAGAAGATGGAAAATTCTACAGAAAAATGATAGAAAGTAATCCTATGCATTCACAAACACATGAAAATCCACGGGAAACTGTGTCCGTTCCATACAGTTCAGTTGTTAGTAGGGAAGAGGAAAAAAGGATCATCATAGAACAGCTGATAACAGCTAAAGAAGCCAGTAGGGGAAACTTTTCATGTATTGCCATTGTTGGGATGGGAGGAGTGGGTAAAACCACTCTTGCACAACTTGTCTTTCAAGATGAAAAGGTTGATGGGTATTTCCGTTTTAGAATTTGGGTTCATGTGGGGAAGGATTTTGAGTTGAAGAGGATTTTCACAGAAATACTGGTCTCTCTAGTGCATTCATCAAATTTGAAAAGAACAAAACCAATAGAGTCTTTACTAAATATGTTAGATGAAAAAAAATCTCAACTCCAAACCCATGTCGATCTCAACTATCCCATCTTCGACAAACCCCAAACCCCTGTCGATCCCAACTCTTCCATCTTCGACAAACTAAATATCTCGTTTGAAGATGCCTGTTCCCTCGAGTTGAACCAACTACCTTCCCCCAATGATCTCAACCCTCTCAACTTGCCCCAACTAAAAATCCTTCATGGTCTCTACTCCAACTACTTAGAACGATTCCAAACCCGTACTGAGAATACTGACTTCACCTCTCTCTCCTTGGACCAACTACAAACTCGCATTCGTGAACTTCTGGCTCGGAAACGATTTTTACTTGTTCTAGACGACCTGTGGAATCATGAAGCATTGCAGCAACTAGTGAATGTCTTAAATGTGGGGGTCAACGGAAGTAAGGTTCTGGTGACTAGTCGGCAGAAAATTGTTTCAAGTTTTATGGATTCAAAGTCACTCCATTCTCTGAAATGTTTGGGTGATGATGAGTCATGGTCGTTGTTCAAAAAATTGGCACTCAAAGAAGATAGTAACTTGAGAGATAGCGAACGTCGGGAGTTTGAAAATTGTGGACGGGAAATTGTACGTATGTGTCAGGGTCTTCCTTTGGCTATAAAACAAATGGCAGGTCTGTTGCGTGGGAAAAACTTGAAAGAATGGAAAAATGTTGCCAAAAGTCAAACATGGAAGGCACGAGCAGAAGATGATACTGGAGTACTGCCTGCTCTTAGACTGAGTTATAATCACCTGCCCTCACCAGATCACAAGCAGTGCTTTTCGTTGTGTTCTCTGTTTCCGAAATCATATCTCTATGAGAAGGATGAGTTGGTCAAGTTATGGATGGCAGAAGGATTTATACAACCACCTGAGAACGGAGTGCGAGAGAGGATAGAAGACATCGGAAGTCGATACTTCAAGGAGCTGTCAGACAGGTTCTTCTTCGAATGCTCAACTGAAGACAAGACAAAGTATAGGATGCATGATCTTATCCATGATCTGGCACTTTCAGTTTCCAGTCCCTTTTGTTGTCAAGTGAATGATATGAAAGATTTTGACGAAAAATCTCGTCATATATCACTGCTTTCAGAACAAGTTGAGAAGCCTGCAATAGAGATCGCCAACAAATCGAAGAAGCTGCGCACACTTTTACTTCCAGTTCAACACTTGCAACTGAGAGCCTTTGGGAAAGGACAAGGTGAGATAATTCATTCACTTACATATATGCGCGTGCTAGACCTGAGTTCAAGCACAGTTGTCACACTTCCAGATTCAATTGGGAGGTTGAAGCTCTTGCGCTACCTCGATTTATCGACAACTGAAATCAGAAAGCTCCCTGACTCGGTATGCAACCTCCTCAATTTGGAGACGCTGAAACTTTTGCGTTGTCCTTGGATTTTCAATTTGCCCAAGAACTTCAAAGCTCTTGTCAACCTGAGACATCTGGAGCTAGATGAAATTTTCTGGTACAAGGCCTTGAAGTTGCCACGAAGCATGGGGTGTCTAACCAGTCTGCACAACTTGCACAAATTTCCGGTTAGCCAAGAAACAGGATACAAACTTGAAGAATTGAAGAACATGGTGTACCTTGGAGGAACGTTGCACATCACAAAGCTGGAAAATGCAGTGTATGCAGGGGAGGCCAACTTGAAAGGGAAAAAAATGATTCAGAAAGTGGTTTATGAATGGAGCAATAAGGATCTTAATTTTCATGATGAAGCCGATGCAAAGCAGGTACTTGAAGACTTGGAACCTCACCCAGAGGCACTCAAAGAGCTCCAAATCTGTCACTACAAGGGCACTGAATTTCCACGATGGATGGGACTGCTCACCAATTTGGTTAGTATTCATTTGAATAATTGCAACAGATCCAAAGTCCTTGATCTTGGGGTGCTACCGAAACTTGAAGAGTTCCGTCTCAAAAATTTGCTAGAGTTGGAGGATTGGAAAGGAACGTTTGTATCCCTCAAAAAGCTAAAGATAGCTAACTGCCCGAATTTGAAAAGTTGTGAAAATTATTCGAAAGGACTTGATTTTCTGAAGATCAAAAGGTGTGAATCATTGGTAAGCCTTAAAATAAACTACCTCGTCCGCCCGAAGAATATCACGCTTGTTGACAATCCTGTTCTGCAGAGGTGGTCTGTAAGCAATCCTCGCCTGTTTGGGCTTTCAATTGGGCTCTTAAGATTGAATGAACTCATAATCATCAATTGCCCCGAGCTACATAAGGTGCCTAACTGCTTCAATCTAGAGAAATTGGAAACAGGTGGGTGCAATTCAGTAATCAAAATGCTAGAAAGAGGTTCTTTATGGAAGTCTCTACAAAAGAAGGAACTTCGACATTTGGCATTGGATACATGTTCTGCAGATGCTAAAACATTAGTGGGTTTGCGGCCTGGTATTATCTCTGGTTTATGGTTCTTGACGATTTCCAATATCTCAAACCTCATACATCTTCCAGATTGGGGTCTCCCCAAACTCAGAGCACTCTACATCCGTGACTGCAAAGCTCTTGAGTATTTATCCAACCAAGAAAACAAGTTGTTCCAAGGATTCACCTCCCTCACAACACTCTCCATCCACAATTGTCCTGAGCTTGTAACGTTGCCAGCTGAAGGCCTCCCAACATCTCTTAAGTATTTGAGTATTGGCTCGTGTGCAAGGCTCGAGTCATTTGGCCCCGCGGATGCACTCCAAAACCTCAACTCCCTCCATGATCTGTACATTGAGGATTGCCCTGCACTCCAGTCCTTTCCAGAGGGTGGGCTACCAACCTCCCTCCTGCATCTCTCTATCCAACGCTGCGCCTCGCTGATAAAACAATGTGGGAAGGAAGGCAGTGATTGGCCCAAGATCAAAGATATCCCTGATCCGGAAATAGGGATGCCATCCACCGAAACTGCAACCTCATCACCATCATCTTCTTCAGCAGCTTGGTACCATCTTGGTGGGAGCTGCGCTTGCGTAGGACAAGCTTCAAAAGGAAAAATGGTTGCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1272

Amino Acids

145.0

Weight (kDa)

8.38

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 103 3.5e-10 Rx N-terminal domain
AAA_16 PF13191 172 - 390 8.5e-06 AAA ATPase domain
NB-ARC PF00931 178 - 273 3e-15 NB-ARC domain
NB-ARC PF00931 345 - 448 1.4e-19 NB-ARC domain
WHD_DRP PF23559 536 - 606 1.9e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 674 - 933 1.1e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 792 - 913 5e-27 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1557
AarI CACCTGC 1 cut(s) 1572
Acc16I TGCGCA 1 cut(s) 1955
Acc36I ACCTGC 4 cut(s) 298, 1433, 1572, 2536
Acc65I GGTACC 1 cut(s) 3750
AccB1I GGYRCC 2 cut(s) 3043, 3750
AccB7I CCANNNNNTGG 2 cut(s) 1813, 3575
AccII CGCG 2 cut(s) 2043, 3497
AccIII TCCGGA 1 cut(s) 3685
AciI CCGC 4 cut(s) 2905, 3205, 3495, 3497
AclI AACGTT 3 cut(s) 2411, 2768, 3416
AclWI GGATC 5 cut(s) 545, 881, 2520, 2676, 3677
AcuI CTGAAG 5 cut(s) 218, 1791, 2873, 3447, 3723
AfaI GTAC 9 cut(s) 312, 1054, 1401, 1533, 2282, 2399, 2550, 3536, 3752
AflII CTTAAG 2 cut(s) 2995, 3446
AflIII ACRYGT 2 cut(s) 400, 3170
AhlI ACTAGT 2 cut(s) 1180, 1229
AjnI CCWGG 1 cut(s) 3208
AjuI GAANNNNNNNTTGG 2 cut(s) 1625, 1657
AloI GAACNNNNNNTCC 2 cut(s) 1203, 1235
Alw21I GWGCWC 2 cut(s) 2592, 3301
Alw26I GTCTC 7 cut(s) 751, 1022, 2174, 2245, 2735, 3135, 3287
AlwI GGATC 5 cut(s) 545, 881, 2520, 2676, 3677
Ama87I CYCGRG 3 cut(s) 944, 3029, 3479
Aor13HI TCCGGA 1 cut(s) 3685
AoxI GGCC 6 cut(s) 2286, 2454, 3206, 3430, 3490, 3659
ArsI GACNNNNNNTTYG 4 cut(s) 1429, 1461, 2226, 2258
Asp700I GAANNNNTTC 2 cut(s) 206, 586
Asp718I GGTACC 1 cut(s) 3750
AspLEI GCGC 5 cut(s) 1956, 2043, 2112, 3620, 3773
AspS9I GGNCC 3 cut(s) 1087, 3491, 3660
AsuII TTCGAA 3 cut(s) 210, 1758, 2834
AvaI CYCGRG 3 cut(s) 944, 3029, 3479
AvaII GGWCC 1 cut(s) 1087
BaeGI GKGCMC 1 cut(s) 2615
BaeI ACNNNNGTAYC 4 cut(s) 1515, 1548, 2757, 2790
BanI GGYRCC 2 cut(s) 3043, 3750
BanII GRGCYC 2 cut(s) 2592, 2994
BarI GAAGNNNNNNTAC 2 cut(s) 1656, 1688
BauI CACGAG 2 cut(s) 1509, 3466
BbsI GAAGAC 4 cut(s) 1409, 1716, 1779, 2562
Bbv12I GWGCWC 2 cut(s) 2592, 3301
BcgI CGANNNNNNTGC 2 cut(s) 1933, 1967
BciT130I CCWGG 1 cut(s) 3210
BciVI GTATCC 3 cut(s) 2360, 2785, 3160
BcoDI GTCTC 7 cut(s) 751, 1022, 2174, 2245, 2735, 3135, 3287
BcuI ACTAGT 2 cut(s) 1180, 1229
BfaI CTAG 9 cut(s) 752, 1145, 1181, 1230, 2048, 2264, 2744, 3062, 3107
BfmI CTRYAG 3 cut(s) 427, 2942, 3177
BfrI CTTAAG 2 cut(s) 2995, 3446
BfuAI ACCTGC 4 cut(s) 298, 1433, 1572, 2536
BfuI GTATCC 3 cut(s) 2360, 2785, 3160
BmcAI AGTACT 1 cut(s) 1533
Bme1390I CCNGG 1 cut(s) 3210
Bme18I GGWCC 1 cut(s) 1087
BmeT110I CYCGRG 3 cut(s) 944, 3029, 3479
BmgT120I GGNCC 3 cut(s) 1087, 3491, 3660
BmiI GGNNCC 4 cut(s) 2565, 3045, 3493, 3752
BmrFI CCNGG 1 cut(s) 3210
BmsI GCATC 9 cut(s) 236, 367, 922, 1782, 2527, 3172, 3490, 3607, 3687
BpiI GAAGAC 4 cut(s) 1409, 1716, 1779, 2562
BpmI CTGGAG 3 cut(s) 1548, 2279, 3542
Bpu10I CCTNAGC 1 cut(s) 3405
Bpu14I TTCGAA 3 cut(s) 210, 1758, 2834
BpuEI CTTGAG 2 cut(s) 1372, 3347
BsaAI YACGTR 1 cut(s) 1403
BsaI GGTCTC 3 cut(s) 751, 1022, 3287
BsaJI CCNNGG 6 cut(s) 479, 1083, 2204, 2401, 3361, 3495
BsaWI WCCGGW 2 cut(s) 2347, 3685
BsaXI ACNNNNNCTCC 2 cut(s) 3503, 3533
Bse1I ACTGG 8 cut(s) 63, 573, 747, 1531, 1830, 1970, 2322, 3559
Bse3DI GCAATG 1 cut(s) 1169
BseAI TCCGGA 1 cut(s) 3685
BseBI CCWGG 1 cut(s) 3210
BseDI CCNNGG 6 cut(s) 479, 1083, 2204, 2401, 3361, 3495
BseMI GCAATG 1 cut(s) 1169
BseMII CTCAG 8 cut(s) 1047, 1541, 1674, 1979, 2045, 2240, 3307, 3396
BseNI ACTGG 8 cut(s) 63, 573, 747, 1531, 1830, 1970, 2322, 3559
BseRI GAGGAG 2 cut(s) 630, 2159
BseSI GKGCMC 1 cut(s) 2615
BsgI GTGCAG 2 cut(s) 2312, 3537
Bsh1236I CGCG 2 cut(s) 2043, 3497
BshFI GGCC 6 cut(s) 2288, 2456, 3208, 3432, 3492, 3661
BshNI GGYRCC 2 cut(s) 3043, 3750
BsiHKAI GWGCWC 2 cut(s) 2592, 3301
BsiHKCI CYCGRG 3 cut(s) 944, 3029, 3479
BsiSI CCGG 2 cut(s) 2348, 3686
BslFI GGGAC 2 cut(s) 1818, 2649
BsmAI GTCTC 7 cut(s) 751, 1022, 2174, 2245, 2735, 3135, 3287
BsmBI CGTCTC 2 cut(s) 2174, 2735
BsmFI GGGAC 2 cut(s) 1818, 2649
BsmI GAATGC 4 cut(s) 457, 757, 1104, 1766
BsnI GGCC 6 cut(s) 2288, 2456, 3208, 3432, 3492, 3661
Bso31I GGTCTC 3 cut(s) 751, 1022, 3287
BsoBI CYCGRG 3 cut(s) 944, 3029, 3479
Bsp119I TTCGAA 3 cut(s) 210, 1758, 2834
Bsp1286I GDGCHC 4 cut(s) 2592, 2615, 2994, 3301
Bsp13I TCCGGA 1 cut(s) 3685
Bsp1407I TGTACA 1 cut(s) 3534
BspACI CCGC 4 cut(s) 2905, 3205, 3495, 3497
BspANI GGCC 6 cut(s) 2288, 2456, 3208, 3432, 3492, 3661
BspCNI CTCAG 8 cut(s) 1048, 1542, 1675, 1980, 2046, 2241, 3306, 3397
BspEI TCCGGA 1 cut(s) 3685
BspFNI CGCG 2 cut(s) 2043, 3497
BspHI TCATGA 2 cut(s) 1162, 2524
BspLI GGNNCC 4 cut(s) 2565, 3045, 3493, 3752
BspMAI CTGCAG 2 cut(s) 2946, 3181
BspMI ACCTGC 4 cut(s) 298, 1433, 1572, 2536
BspPI GGATC 5 cut(s) 545, 881, 2520, 2676, 3677
BspT104I TTCGAA 3 cut(s) 210, 1758, 2834
BspT107I GGYRCC 2 cut(s) 3043, 3750
BspTI CTTAAG 2 cut(s) 2995, 3446
BspTNI GGTCTC 3 cut(s) 751, 1022, 3287
BsrDI GCAATG 1 cut(s) 1169
BsrGI TGTACA 1 cut(s) 3534
BsrI ACTGG 8 cut(s) 63, 573, 747, 1531, 1830, 1970, 2322, 3559
BssECI CCNNGG 6 cut(s) 479, 1083, 2204, 2401, 3361, 3495
BssSI CACGAG 2 cut(s) 1509, 3466
BssT1I CCWWGG 4 cut(s) 1083, 2204, 2401, 3361
Bst2BI CACGAG 2 cut(s) 1509, 3466
Bst2UI CCWGG 1 cut(s) 3210
Bst4CI ACNGT 4 cut(s) 257, 490, 506, 2068
Bst6I CTCTTC 4 cut(s) 519, 716, 901, 2715
BstAFI CTTAAG 2 cut(s) 2995, 3446
BstAPI GCANNNNNTGC 2 cut(s) 2335, 2444
BstAUI TGTACA 1 cut(s) 3534
BstBAI YACGTR 1 cut(s) 1403
BstBI TTCGAA 3 cut(s) 210, 1758, 2834
BstC8I GCNNGC 5 cut(s) 1539, 1920, 2045, 3423, 3775
BstDSI CCRYGG 2 cut(s) 479, 3495
BstFNI CGCG 2 cut(s) 2043, 3497
BstHHI GCGC 5 cut(s) 1956, 2043, 2112, 3620, 3773
BstMAI GTCTC 7 cut(s) 751, 1022, 2174, 2245, 2735, 3135, 3287
BstMWI GCNNNNNNNGC 9 cut(s) 68, 301, 304, 569, 2335, 2444, 2453, 2967, 3774
BstNI CCWGG 1 cut(s) 3210
BstNSI RCATGY 2 cut(s) 404, 3174
BstSCI CCNGG 1 cut(s) 3208
BstSFI CTRYAG 3 cut(s) 427, 2942, 3177
BstSLI GKGCMC 1 cut(s) 2615
BstSNI TACGTA 1 cut(s) 1403
BstUI CGCG 2 cut(s) 2043, 3497
BstV2I GAAGAC 4 cut(s) 1409, 1716, 1779, 2562
BstX2I RGATCY 2 cut(s) 2512, 2681
BstXI CCANNNNNNTGG 1 cut(s) 3761
BstYI RGATCY 2 cut(s) 2512, 2681
BsuI GTATCC 3 cut(s) 2360, 2785, 3160
BsuRI GGCC 6 cut(s) 2288, 2456, 3208, 3432, 3492, 3661
BtgI CCRYGG 2 cut(s) 479, 3495
BtsI GCAGTG 4 cut(s) 1592, 1892, 2445, 3658
BtsIMutI CAGTG 5 cut(s) 1592, 1892, 2445, 2613, 3658
BveI ACCTGC 4 cut(s) 298, 1433, 1572, 2536
Cac8I GCNNGC 5 cut(s) 1539, 1920, 2045, 3423, 3775
CciI TCATGA 2 cut(s) 1162, 2524
CfoI GCGC 5 cut(s) 1956, 2043, 2112, 3620, 3773
Cfr13I GGNCC 3 cut(s) 1087, 3491, 3660
Cfr42I CCGCGG 1 cut(s) 3498
CseI GACGC 1 cut(s) 2191
Csp6I GTAC 9 cut(s) 311, 1053, 1400, 1532, 2281, 2398, 2549, 3535, 3751
CviQI GTAC 9 cut(s) 311, 1053, 1400, 1532, 2281, 2398, 2549, 3535, 3751
Eam1104I CTCTTC 4 cut(s) 519, 716, 901, 2715
EarI CTCTTC 4 cut(s) 519, 716, 901, 2715
EciI GGCGGA 1 cut(s) 2894
Ecl136II GAGCTC 1 cut(s) 2590
Eco105I TACGTA 1 cut(s) 1403
Eco130I CCWWGG 4 cut(s) 1083, 2204, 2401, 3361
Eco147I AGGCCT 2 cut(s) 2288, 3432
Eco24I GRGCYC 2 cut(s) 2592, 2994
Eco31I GGTCTC 3 cut(s) 751, 1022, 3287
Eco32I GATATC 1 cut(s) 3676
Eco47I GGWCC 1 cut(s) 1087
Eco53kI GAGCTC 1 cut(s) 2590
Eco57I CTGAAG 5 cut(s) 218, 1791, 2873, 3447, 3723
Eco88I CYCGRG 3 cut(s) 944, 3029, 3479
EcoICRI GAGCTC 1 cut(s) 2590
EcoRI GAATTC 2 cut(s) 80, 175
EcoRII CCWGG 1 cut(s) 3208
EcoRV GATATC 1 cut(s) 3676
EcoT14I CCWWGG 4 cut(s) 1083, 2204, 2401, 3361
EcoT22I ATGCAT 2 cut(s) 459, 1797
EcoT38I GRGCYC 2 cut(s) 2592, 2994
ErhI CCWWGG 4 cut(s) 1083, 2204, 2401, 3361
Esp3I CGTCTC 2 cut(s) 2174, 2735
FalI AAGNNNNNCTT 8 cut(s) 129, 161, 335, 367, 1574, 1606, 3103, 3135
FaqI GGGAC 2 cut(s) 1818, 2649
FauI CCCGC 1 cut(s) 3502
FriOI GRGCYC 2 cut(s) 2592, 2994
FspBI CTAG 9 cut(s) 752, 1145, 1181, 1230, 2048, 2264, 2744, 3062, 3107
FspI TGCGCA 1 cut(s) 1955
GlaI GCGC 5 cut(s) 1955, 2042, 2111, 3619, 3772
GsuI CTGGAG 3 cut(s) 1548, 2279, 3542
HaeIII GGCC 6 cut(s) 2288, 2456, 3208, 3432, 3492, 3661
HapII CCGG 2 cut(s) 2348, 3686
HgaI GACGC 1 cut(s) 2191
HhaI GCGC 5 cut(s) 1956, 2043, 2112, 3620, 3773
Hin6I GCGC 5 cut(s) 1954, 2041, 2110, 3618, 3771
HinP1I GCGC 5 cut(s) 1954, 2041, 2110, 3618, 3771
HincII GTYRAC 3 cut(s) 1207, 2245, 2929
HindII GTYRAC 3 cut(s) 1207, 2245, 2929
HindIII AAGCTT 1 cut(s) 3786
HpaII CCGG 2 cut(s) 2348, 3686
Hpy166II GTNNAC 7 cut(s) 313, 1112, 1207, 1388, 2245, 2398, 2929
Hpy8I GTNNAC 7 cut(s) 313, 1112, 1207, 1388, 2245, 2398, 2929
Hpy99I CGWCG 1 cut(s) 1371
HpyCH4III ACNGT 4 cut(s) 257, 490, 506, 2068
HpyCH4IV ACGT 5 cut(s) 1366, 1402, 2411, 2768, 3416
HpyF10VI GCNNNNNNNGC 9 cut(s) 68, 301, 304, 569, 2335, 2444, 2453, 2967, 3774
HpySE526I ACGT 5 cut(s) 1366, 1402, 2411, 2768, 3416
HspAI GCGC 5 cut(s) 1954, 2041, 2110, 3618, 3771
Kpn2I TCCGGA 1 cut(s) 3685
KpnI GGTACC 1 cut(s) 3754
KspI CCGCGG 1 cut(s) 3498
LmnI GCTCC 6 cut(s) 1735, 2151, 2260, 2502, 2595, 3765
LweI GCATC 9 cut(s) 236, 367, 922, 1782, 2527, 3172, 3490, 3607, 3687
MaeI CTAG 9 cut(s) 752, 1145, 1181, 1230, 2048, 2264, 2744, 3062, 3107
MaeII ACGT 5 cut(s) 1366, 1402, 2411, 2768, 3416
MaeIII GTNAC 7 cut(s) 1225, 1269, 1346, 2071, 2600, 3311, 3412
MfeI CAATTG 4 cut(s) 2088, 2985, 3022, 3397
MflI RGATCY 2 cut(s) 2512, 2681
MhlI GDGCHC 4 cut(s) 2592, 2615, 2994, 3301
MlyI GAGTC 4 cut(s) 800, 1313, 2147, 3491
MmeI TCCRAC 4 cut(s) 1050, 2730, 3363, 3634
Mph1103I ATGCAT 2 cut(s) 459, 1797
MroI TCCGGA 1 cut(s) 3685
MroXI GAANNNNTTC 2 cut(s) 206, 586
MseI TTAA 8 cut(s) 147, 342, 408, 1193, 2517, 2886, 2996, 3447
MslI CAYNNNNRTG 1 cut(s) 3759
MspA1I CMGCKG 3 cut(s) 553, 3425, 3497
MspCI CTTAAG 2 cut(s) 2995, 3446
MspI CCGG 2 cut(s) 2348, 3686
MspR9I CCNGG 1 cut(s) 3210
MunI CAATTG 4 cut(s) 2088, 2985, 3022, 3397
Mva1269I GAATGC 4 cut(s) 457, 757, 1104, 1766
MvaI CCWGG 1 cut(s) 3210
MvnI CGCG 2 cut(s) 2043, 3497
MwoI GCNNNNNNNGC 9 cut(s) 68, 301, 304, 569, 2335, 2444, 2453, 2967, 3774
NlaIV GGNNCC 4 cut(s) 2565, 3045, 3493, 3752
NmuCI GTSAC 5 cut(s) 1225, 1269, 2071, 2600, 3311
NsbI TGCGCA 1 cut(s) 1955
NsiI ATGCAT 2 cut(s) 459, 1797
NspI RCATGY 2 cut(s) 404, 3174
NspV TTCGAA 3 cut(s) 210, 1758, 2834
PaeR7I CTCGAG 2 cut(s) 944, 3479
PagI TCATGA 2 cut(s) 1162, 2524
PaqCI CACCTGC 1 cut(s) 1572
PceI AGGCCT 2 cut(s) 2288, 3432
PciI ACATGT 2 cut(s) 400, 3170
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 4 cut(s) 457, 757, 1104, 1766
PdmI GAANNNNTTC 2 cut(s) 206, 586
PfeI GAWTC 7 cut(s) 1041, 1159, 1262, 2084, 2479, 2870, 3366
PflMI CCANNNNNTGG 2 cut(s) 1813, 3575
PleI GAGTC 4 cut(s) 799, 1312, 2147, 3490
PpsI GAGTC 4 cut(s) 799, 1312, 2147, 3490
Ppu21I YACGTR 1 cut(s) 1403
PscI ACATGT 2 cut(s) 400, 3170
PsiI TTATAA 1 cut(s) 1557
Psp124BI GAGCTC 1 cut(s) 2592
Psp1406I AACGTT 3 cut(s) 2411, 2768, 3416
Psp6I CCWGG 1 cut(s) 3208
PspGI CCWGG 1 cut(s) 3208
PspN4I GGNNCC 4 cut(s) 2565, 3045, 3493, 3752
PspPI GGNCC 3 cut(s) 1087, 3491, 3660
PspXI VCTCGAGB 2 cut(s) 944, 3479
PsrI GAACNNNNNNTAC 2 cut(s) 2381, 2413
PstI CTGCAG 2 cut(s) 2946, 3181
PsuI RGATCY 2 cut(s) 2512, 2681
PvuII CAGCTG 2 cut(s) 553, 3425
RsaI GTAC 9 cut(s) 312, 1054, 1401, 1533, 2282, 2399, 2550, 3536, 3752
RsaNI GTAC 9 cut(s) 311, 1053, 1400, 1532, 2281, 2398, 2549, 3535, 3751
RseI CAYNNNNRTG 1 cut(s) 3759
SacI GAGCTC 1 cut(s) 2592
SacII CCGCGG 1 cut(s) 3498
SaqAI TTAA 8 cut(s) 147, 342, 408, 1193, 2517, 2886, 2996, 3447
Sau96I GGNCC 3 cut(s) 1087, 3491, 3660
ScaI AGTACT 1 cut(s) 1533
SchI GAGTC 4 cut(s) 800, 1313, 2147, 3491
ScrFI CCNGG 1 cut(s) 3210
SduI GDGCHC 4 cut(s) 2592, 2615, 2994, 3301
SfaNI GCATC 9 cut(s) 236, 367, 922, 1782, 2527, 3172, 3490, 3607, 3687
SfcI CTRYAG 3 cut(s) 427, 2942, 3177
Sfr274I CTCGAG 2 cut(s) 944, 3479
Sfr303I CCGCGG 1 cut(s) 3498
SfuI TTCGAA 3 cut(s) 210, 1758, 2834
SgrBI CCGCGG 1 cut(s) 3498
SinI GGWCC 1 cut(s) 1087
SlaI CTCGAG 2 cut(s) 944, 3479
SmiMI CAYNNNNRTG 1 cut(s) 3759
SmlI CTYRAG 6 cut(s) 944, 1351, 2995, 3326, 3446, 3479
SmoI CTYRAG 6 cut(s) 944, 1351, 2995, 3326, 3446, 3479
SnaBI TACGTA 1 cut(s) 1403
SpeI ACTAGT 2 cut(s) 1180, 1229
SseBI AGGCCT 2 cut(s) 2288, 3432
SsiI CCGC 4 cut(s) 2905, 3205, 3495, 3497
SspMI CTAG 9 cut(s) 752, 1145, 1181, 1230, 2048, 2264, 2744, 3062, 3107
SstI GAGCTC 1 cut(s) 2592
StuI AGGCCT 2 cut(s) 2288, 3432
StyD4I CCNGG 1 cut(s) 3208
StyI CCWWGG 4 cut(s) 1083, 2204, 2401, 3361
TaaI ACNGT 4 cut(s) 257, 490, 506, 2068
TaiI ACGT 5 cut(s) 1369, 1405, 2414, 2771, 3419
TatI WGTACW 2 cut(s) 1531, 3534
TauI GCSGC 1 cut(s) 3208
TfiI GAWTC 7 cut(s) 1041, 1159, 1262, 2084, 2479, 2870, 3366
Tru1I TTAA 8 cut(s) 147, 342, 408, 1193, 2517, 2886, 2996, 3447
Tru9I TTAA 8 cut(s) 147, 342, 408, 1193, 2517, 2886, 2996, 3447
TscAI CASTG 5 cut(s) 1592, 1899, 2445, 2620, 3658
TseFI GTSAC 5 cut(s) 1225, 1269, 2071, 2600, 3311
Tsp45I GTSAC 5 cut(s) 1225, 1269, 2071, 2600, 3311
TspGWI ACGGA 6 cut(s) 484, 668, 1224, 1704, 2717, 3299
TspRI CASTG 5 cut(s) 1592, 1899, 2445, 2620, 3658
Van91I CCANNNNNTGG 2 cut(s) 1813, 3575
Vha464I CTTAAG 2 cut(s) 2995, 3446
VpaK11BI GGWCC 1 cut(s) 1087
XbaI TCTAGA 2 cut(s) 1144, 3061
XceI RCATGY 2 cut(s) 404, 3174
XcmI CCANNNNNNNNNTGG 2 cut(s) 329, 607
XhoI CTCGAG 2 cut(s) 944, 3479
XmnI GAANNNNTTC 2 cut(s) 206, 586
XspI CTAG 9 cut(s) 752, 1145, 1181, 1230, 2048, 2264, 2744, 3062, 3107
ZrmI AGTACT 1 cut(s) 1533
Zsp2I ATGCAT 2 cut(s) 459, 1797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.