Rh2DG403600

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005191
Physical Location & Seq
Forward (+)
58765247 .. 58770838
5592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG403600.1

Sequence Viewer

Length: 3435 bp
ATGGCCGAAATAGCAACTCACGCTGTGGGGATTGCCATCACTAAATTGTCTCATCTTGTCACCACGGAGTTTCTTGCATTCACTAATGTCAAGGATGACCTGGAATCCCTCAGAAGCACTCTAACAGATGTTGGGGAGTTGCTTGGAATTCTGGAAAGAAATCGGACGAATTCTTCAGGTTTCGAACAGATGAACAATTGGCTCAAGAAGCTTCAAGTAGCAGCTTATGATGCAGGGGATTTAATCGCGTCCTGGGCAGTAGAATATCACCAATGGAAGACGAAGAAGCAGGTACGAAAATGCTCTCTTCCATTTAGTGCCTCAAAATTTTTCTTTCAGCATCAAGAATCATGTGACTTGAGAGAAATTACAGCGAGAATAAATAAGATTTTACAAGATGGACAAGTCTATAGAACAATCATTGGATTGCCTAGGAGTGAAAGTCTTTCACGGCAAACTGGCTCTCTTTCTAGCAATATAATTGAGGGCAGGGGAGATGATGTAGAGAACATAATAAAACTGCTGATACCAGATGAAGGAGCCAGTGATGAGGGCAGTATTGCTTTTATTCCAATTGTGGGGATGGGGGGCTTGGGTAAAACAACTCTCGCTCAACTCGTATATAAGAATGACAGGGTTCATGATCATTTTAAAAGAAAATTCTGGGTTTGTGTCACAGAAACTTATGACAAGACTAGAATTCTCAAAGGAATGTTGGAGTCTAATGAGATTACTTTTGATGCCAATATCACATTCGAGGTACTGCAAGACCGAGTTCGTGAACTCGTGGCTGAAAAACGATTTTTACTTGTTCTAGATGATTTATGGAACAATAATTATATGGATTTGGAGCCACTAGAAAATTTACTGAAACAAGGGGTCAGTGGAAGTAAGGTCTTGGTTACTAGTCGGAACAATGAAGTTCGGAAGATTACGGGTGCAAAATCCTCCTATAGCTTGCGACATTTCAATGATAAAGAATCACTGTCGTTATTCGAAAAAATTGCCTTTGAAGGAAGGAGTCCGCCAGATGAGTTGAAGGAATATGCAAAGCAAATTGTAAACAAGTGCAACGGTTTACCTCTGGCAGTAAAACAAATGGGGGGTCTGCTACGTGGAATCACTGATGCAAGTGAGTGGAAATATGTCGCTAGAAGTGAAATATGGGAACTAGAAAAAGATAAAGTACTGCCTGCTCTTCGATTGAGTTATAATCATCTGTCTTCAGCTCTGAAGCAATGTTTTGCGTATTGTTCCCTGTTTCCAAAAGCTTATGTTTTTGAAAAAAATGAGTTGATCAAATTATGGATGGCAGAAGCATTCATTCAACCTCATGACAGACACAGGACAGAAGACATAGGAAGCAGATACTTCAAGGAGCTGTCAGACAGGTTCTTCTTTGAGATCTCGGCGGAAGACAATAGCAAGTGTAGAATGCATGATCTTATCCACGACTTGGCGCTATTAATTTCCTCTCCCTTTTTCTGCCAAGTGAAAGATACTGTGCCAAAGAGTTTAGACGAAGTATCTCGCCATGTATCACTGCTTTCGAATGAGGTTGAGCAGCCCCTTTCAGAAATAATAAAAAAATCAAAGAAGCTGCGCACACTTTTATTGCCAGTGAAACACTTCAAAGCCTTTGGGAAAGCTCAACTTGCAATATTCCATTCACTTAAGTACATGCGCACGCTAGATTTGAGTTCAAGCACACTTCAAGAACTGTCAGGCTCAATCGAGAATTTGAAGCTTTTGCACTACCTTGATTTATCAAAAACTGAAATAAAAAAGCTTCCCGATTCGATATGCAACCTCTACCATTTGGAGACGTTGAAACTTTCAGATTGCCCTTGGCTTTTCACTTTGCCCAGGAAGCTCAAAACTCTTGTCAACCTGAGACATCTGGAGCTAGATGAAATGTTCTGGTACAAGGTATCAACGCTTCCAAAAAGCATGGGGTGTCTAACCAGTCTACACAATCTTCACAAATTTCAGGTTGGCTGCAATACAGGATACAAACTTCAAGAATTGAAGAACATGGAGTACCTTACAGGGTTCCTGCACATCTCAAACCTGGAAAATGCAGTGGATGCAGGGGAGGCCAACTTGAAAGAAAAAGAAATGATTGATAAAGTGGTATATGAATGGAGCAGCAGCAATCTGAATTTGCAAGATGAAGACGCCAAGCAGGTACTCGAAGACCTGCAGCCTCACCCGAGGGTTCAAGAGATCCAAATCTGTCACTACAGAAGCTGTGAGTTTCCAATTTGGATCAGATACGGAAGGTATGAGAACTTAACTTCTATATATTTGAATCATTGCACAAGGATCAAAATCCTCTCACTTGGCGAGGTACCCAACCTCAGAGAACTCCGTCTCAAGAACATGGCAGAAATGATGGAATGGAAGGAAGAGAACAACGTGTTCCTCTTGAGTCTAAAGATTAGTGGCTGCCCAAAGCTGACAAAACTGCCCGGCTACTTTCCTGTATTACATAGTATGAAGATCAAGAATTGTGAATCATTGGATACCCTTCCAGTTGGCCCAGTGGAATTCATAAGACTTGCTGGCAATCCTGTGCTGAAGCACTGGACTGAAGTCAATGTACTAACAAGGAGAGTTGTCGATGGTCGCCCGGTCACAAGTCATGGCATGACAAGTCATTATCTGTTGGAAGCAAACATTATCAACTGCCCTGAACTGCAGAGGTTGCCAAGAGAACTCCATCCACAGAAACTGGAAATAAGCGGCTGCAAGAACTTGCGCACCCTACCAGATGCAGACCATGCAGGATGGCTTGGGGTTTTAGCATTGGATGCATGCCATGATGAAACATTAGTTGGTATGATCCCCAGTTCTGGGAGTTTATATTCCTTGGTGATTTCTAACATCCCAAATCTCATTTGTCTTCCAAAGTGGCCCAGTCTACCCAGCCTCCAAGCATTGTATATCCGTGATTGCACAGACCTTGAATATCTATTCAACCAAGAGAATGGGAACGGGTTGTTCGATGGCTACAACTCTCTAAAGCACCTCTCCATCCGTAACTGTCCTAAACTTCTGACACTGCCTGCTGAAGGTCTCCCAGCCAGTCTTCAAGTTTTGAGTATTGGCCAATGTGAAAGTCTCAGTTCGTTTGGCCCCAGGGAAGTACTCGCAAAACTCACATCCCTTGGTGATCTCTACCTTGAAGATTGCCCTGCACTCCAGTCCTTGCCAGAGGAAGGCCTGTCAGCGTCCCTCCAACATCTGTCCATCCAAGGATGCCCCTCGCTGATAAGAAGCTGTGCAAAAGATGGCAGCGATTGGCCAAAGATCAAGGACATCCCCGATCTGGAAATAGAGATGCCATCTACTGAAACCACTTCAGCACCATGGTACAATCCTTTTAGGAACTGCGGAGGAAACAAGTCCAAAGGAAAGAAGGCTTCGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1144

Amino Acids

129.88

Weight (kDa)

6.65

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 97 1.3e-12 Rx N-terminal domain
NB-ARC PF00931 168 - 340 1.7e-30 NB-ARC domain
WHD_DRP PF23559 420 - 487 1.2e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 551 - 802 3.5e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 673 - 795 1.6e-27 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1212
Acc16I TGCGCA 3 cut(s) 1604, 1685, 2762
Acc36I ACCTGC 3 cut(s) 280, 2176, 2208
Acc65I GGTACC 1 cut(s) 2350
AccB1I GGYRCC 1 cut(s) 2350
AccB7I CCANNNNNTGG 1 cut(s) 1456
AccI GTMKAC 2 cut(s) 1969, 2923
AccII CGCG 1 cut(s) 248
AciI CCGC 4 cut(s) 1025, 1412, 2745, 3396
AclWI GGATC 4 cut(s) 2221, 2276, 2333, 2839
AcoI YGGCCR 3 cut(s) 3, 3109, 3305
AcuI CTGAAG 7 cut(s) 159, 1209, 1253, 2600, 2613, 3093, 3348
AcyI GRCGYC 1 cut(s) 2178
AdeI CACNNNGTG 1 cut(s) 25
AfiI CCNNNNNNNGG 8 cut(s) 536, 578, 1456, 2855, 2856, 3074, 3221, 3331
AflII CTTAAG 1 cut(s) 1673
AflIII ACRYGT 1 cut(s) 2418
AhlI ACTAGT 1 cut(s) 905
AjnI CCWGG 5 cut(s) 99, 251, 1865, 2070, 3140
AjuI GAANNNNNNNTTGG 4 cut(s) 737, 769, 2820, 2852
Alw26I GTCTC 6 cut(s) 54, 1817, 1888, 2378, 3083, 3128
AlwI GGATC 4 cut(s) 2221, 2276, 2333, 2839
AlwNI CAGNNNCTG 2 cut(s) 2250, 2734
Ama87I CYCGRG 1 cut(s) 2212
AoxI GGCC 8 cut(s) 3, 2097, 2539, 2915, 3109, 3136, 3223, 3305
ArsI GACNNNNNNTTYG 4 cut(s) 1090, 1122, 1869, 1901
AseI ATTAAT 1 cut(s) 1466
Asp700I GAANNNNTTC 1 cut(s) 1628
Asp718I GGTACC 1 cut(s) 2350
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 4 cut(s) 1462, 1605, 1686, 2763
AspS9I GGNCC 3 cut(s) 2540, 2916, 3137
AsuC2I CCSGG 2 cut(s) 2472, 2633
AsuHPI GGTGA 5 cut(s) 52, 260, 2201, 2887, 3185
AsuII TTCGAA 3 cut(s) 183, 996, 1550
AvaI CYCGRG 1 cut(s) 2212
AvrII CCTAGG 1 cut(s) 431
BalI TGGCCA 2 cut(s) 3111, 3307
BanI GGYRCC 1 cut(s) 2350
BarI GAAGNNNNNNTAC 4 cut(s) 1923, 1955, 2586, 2618
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 8 cut(s) 284, 1215, 1359, 1422, 2181, 2202, 2897, 3083
BceAI ACGGC 1 cut(s) 467
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciT130I CCWGG 5 cut(s) 101, 253, 1867, 2072, 3142
BciVI GTATCC 2 cut(s) 2003, 2518
BclI TGATCA 2 cut(s) 643, 1296
BcnI CCSGG 2 cut(s) 2472, 2633
BcoDI GTCTC 6 cut(s) 54, 1817, 1888, 2378, 3083, 3128
BcuI ACTAGT 1 cut(s) 905
BfmI CTRYAG 5 cut(s) 409, 952, 2201, 2242, 2699
BfoI RGCGCY 1 cut(s) 1463
BfrI CTTAAG 1 cut(s) 1673
BfuAI ACCTGC 3 cut(s) 280, 2176, 2208
BfuI GTATCC 2 cut(s) 2003, 2518
BglII AGATCT 1 cut(s) 1404
BlnI CCTAGG 1 cut(s) 431
BmcAI AGTACT 2 cut(s) 1188, 3150
Bme1390I CCNGG 7 cut(s) 101, 253, 1867, 2072, 2472, 2633, 3142
BmeT110I CYCGRG 1 cut(s) 2212
BmgT120I GGNCC 3 cut(s) 2540, 2916, 3137
BmiI GGNNCC 5 cut(s) 541, 852, 2054, 2352, 3139
BmrFI CCNGG 7 cut(s) 101, 253, 1867, 2072, 2472, 2633, 3142
BmrI ACTGGG 3 cut(s) 2537, 2844, 2913
BmsI GCATC 9 cut(s) 220, 349, 730, 1117, 2077, 2764, 2803, 3251, 3333
BmuI ACTGGG 3 cut(s) 2537, 2844, 2913
BoxI GACNNNNGTC 1 cut(s) 2594
BpiI GAAGAC 8 cut(s) 284, 1215, 1359, 1422, 2181, 2202, 2897, 3083
BpmI CTGGAG 2 cut(s) 1922, 3188
Bpu14I TTCGAA 3 cut(s) 183, 996, 1550
BpuEI CTTGAG 4 cut(s) 188, 379, 2360, 2449
BpuMI CCSGG 2 cut(s) 2472, 2633
BsaAI YACGTR 1 cut(s) 1115
BsaBI GATNNNNATC 3 cut(s) 35, 2231, 2330
BsaHI GRCGYC 1 cut(s) 2178
BsaI GGTCTC 1 cut(s) 3083
BsaXI ACNNNNNCTCC 6 cut(s) 427, 457, 486, 516, 2916, 2946
Bsc4I CCNNNNNNNGG 8 cut(s) 536, 578, 1456, 2855, 2856, 3074, 3221, 3331
Bse3DI GCAATG 2 cut(s) 1244, 2314
Bse8I GATNNNNATC 3 cut(s) 35, 2231, 2330
BseBI CCWGG 5 cut(s) 101, 253, 1867, 2072, 3142
BseJI GATNNNNATC 3 cut(s) 35, 2231, 2330
BseLI CCNNNNNNNGG 8 cut(s) 536, 578, 1456, 2855, 2856, 3074, 3221, 3331
BseMI GCAATG 2 cut(s) 1244, 2314
BseMII CTCAG 4 cut(s) 124, 1883, 2374, 3139
BseYI CCCAGC 2 cut(s) 2927, 3082
BsgI GTGCAG 2 cut(s) 2042, 3183
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 8 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3225, 3307
BshNI GGYRCC 1 cut(s) 2350
BsiHKCI CYCGRG 1 cut(s) 2212
BsiSI CCGG 2 cut(s) 2472, 2633
BslFI GGGAC 1 cut(s) 3220
BslI CCNNNNNNNGG 8 cut(s) 536, 578, 1456, 2855, 2856, 3074, 3221, 3331
BsmAI GTCTC 6 cut(s) 54, 1817, 1888, 2378, 3083, 3128
BsmBI CGTCTC 2 cut(s) 1817, 2378
BsmFI GGGAC 1 cut(s) 3220
BsmI GAATGC 3 cut(s) 77, 1319, 1440
BsnI GGCC 8 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3225, 3307
Bso31I GGTCTC 1 cut(s) 3083
BsoBI CYCGRG 1 cut(s) 2212
Bsp119I TTCGAA 3 cut(s) 183, 996, 1550
Bsp19I CCATGG 1 cut(s) 3371
BspACI CCGC 4 cut(s) 1025, 1412, 2745, 3396
BspANI GGCC 8 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3225, 3307
BspCNI CTCAG 4 cut(s) 123, 1884, 2373, 3138
BspFNI CGCG 1 cut(s) 248
BspHI TCATGA 2 cut(s) 640, 1333
BspLI GGNNCC 5 cut(s) 541, 852, 2054, 2352, 3139
BspMAI CTGCAG 2 cut(s) 2205, 2703
BspMI ACCTGC 3 cut(s) 280, 2176, 2208
BspPI GGATC 4 cut(s) 2221, 2276, 2333, 2839
BspQI GCTCTTC 1 cut(s) 1203
BspT104I TTCGAA 3 cut(s) 183, 996, 1550
BspT107I GGYRCC 1 cut(s) 2350
BspTI CTTAAG 1 cut(s) 1673
BspTNI GGTCTC 1 cut(s) 3083
BsrDI GCAATG 2 cut(s) 1244, 2314
BssNI GRCGYC 1 cut(s) 2178
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 6 cut(s) 431, 1847, 2871, 3169, 3256, 3371
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 5 cut(s) 101, 253, 1867, 2072, 3142
Bst4CI ACNGT 5 cut(s) 987, 1076, 1504, 1722, 3047
Bst6I CTCTTC 3 cut(s) 312, 1203, 2403
BstACI GRCGYC 1 cut(s) 2178
BstAFI CTTAAG 1 cut(s) 1673
BstAPI GCANNNNNTGC 4 cut(s) 2087, 2707, 2783, 2813
BstBAI YACGTR 1 cut(s) 1115
BstBI TTCGAA 3 cut(s) 183, 996, 1550
BstC8I GCNNGC 6 cut(s) 959, 1194, 1688, 2566, 2818, 3069
BstDEI CTNAG 4 cut(s) 110, 1892, 2360, 3125
BstDSI CCRYGG 2 cut(s) 63, 3371
BstENI CCTNNNNNAGG 1 cut(s) 3072
BstFNI CGCG 1 cut(s) 248
BstH2I RGCGCY 1 cut(s) 1463
BstHHI GCGC 4 cut(s) 1462, 1605, 1686, 2763
BstMAI GTCTC 6 cut(s) 54, 1817, 1888, 2378, 3083, 3128
BstNI CCWGG 5 cut(s) 101, 253, 1867, 2072, 3142
BstNSI RCATGY 2 cut(s) 1684, 2820
BstPAI GACNNNNGTC 1 cut(s) 2594
BstSCI CCNGG 7 cut(s) 99, 251, 1865, 2070, 2470, 2631, 3140
BstSFI CTRYAG 5 cut(s) 409, 952, 2201, 2242, 2699
BstUI CGCG 1 cut(s) 248
BstV2I GAAGAC 8 cut(s) 284, 1215, 1359, 1422, 2181, 2202, 2897, 3083
BstX2I RGATCY 2 cut(s) 1404, 2226
BstXI CCANNNNNNTGG 1 cut(s) 2990
BstYI RGATCY 2 cut(s) 1404, 2226
BsuI GTATCC 2 cut(s) 2003, 2518
BsuRI GGCC 8 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3225, 3307
BtgI CCRYGG 2 cut(s) 63, 3371
BtsI GCAGTG 3 cut(s) 1541, 2088, 3062
BveI ACCTGC 3 cut(s) 280, 2176, 2208
Cac8I GCNNGC 6 cut(s) 959, 1194, 1688, 2566, 2818, 3069
CaiI CAGNNNCTG 2 cut(s) 2250, 2734
CciI TCATGA 2 cut(s) 640, 1333
CfoI GCGC 4 cut(s) 1462, 1605, 1686, 2763
Cfr13I GGNCC 3 cut(s) 2540, 2916, 3137
CseI GACGC 3 cut(s) 237, 2186, 3222
DdeI CTNAG 4 cut(s) 110, 1892, 2360, 3125
DraI TTTAAA 1 cut(s) 652
DraIII CACNNNGTG 1 cut(s) 25
EaeI YGGCCR 3 cut(s) 3, 3109, 3305
Eam1104I CTCTTC 3 cut(s) 312, 1203, 2403
EarI CTCTTC 3 cut(s) 312, 1203, 2403
EciI GGCGGA 2 cut(s) 1014, 1427
Eco130I CCWWGG 6 cut(s) 431, 1847, 2871, 3169, 3256, 3371
Eco147I AGGCCT 1 cut(s) 3225
Eco31I GGTCTC 1 cut(s) 3083
Eco57I CTGAAG 7 cut(s) 159, 1209, 1253, 2600, 2613, 3093, 3348
Eco88I CYCGRG 1 cut(s) 2212
EcoNI CCTNNNNNAGG 1 cut(s) 3072
EcoRI GAATTC 4 cut(s) 147, 169, 699, 2549
EcoRII CCWGG 5 cut(s) 99, 251, 1865, 2070, 3140
EcoT14I CCWWGG 6 cut(s) 431, 1847, 2871, 3169, 3256, 3371
EcoT22I ATGCAT 2 cut(s) 1440, 2818
ErhI CCWWGG 6 cut(s) 431, 1847, 2871, 3169, 3256, 3371
Esp3I CGTCTC 2 cut(s) 1817, 2378
FalI AAGNNNNNCTT 2 cut(s) 1638, 1670
FaqI GGGAC 1 cut(s) 3220
FbaI TGATCA 2 cut(s) 643, 1296
FblI GTMKAC 2 cut(s) 1969, 2923
FspAI RTGCGCAY 1 cut(s) 1685
FspI TGCGCA 3 cut(s) 1604, 1685, 2762
GlaI GCGC 4 cut(s) 1461, 1604, 1685, 2762
GsaI CCCAGC 2 cut(s) 2931, 3086
GsuI CTGGAG 2 cut(s) 1922, 3188
HaeII RGCGCY 1 cut(s) 1463
HaeIII GGCC 8 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3225, 3307
HapII CCGG 2 cut(s) 2472, 2633
HgaI GACGC 3 cut(s) 237, 2186, 3222
HhaI GCGC 4 cut(s) 1462, 1605, 1686, 2763
Hin1I GRCGYC 1 cut(s) 2178
Hin6I GCGC 4 cut(s) 1460, 1603, 1684, 2761
HinP1I GCGC 4 cut(s) 1460, 1603, 1684, 2761
HincII GTYRAC 1 cut(s) 1888
HindII GTYRAC 1 cut(s) 1888
HindIII AAGCTT 4 cut(s) 209, 1269, 1745, 1787
HpaII CCGG 2 cut(s) 2472, 2633
HphI GGTGA 5 cut(s) 52, 260, 2201, 2887, 3185
Hpy166II GTNNAC 6 cut(s) 782, 1063, 1079, 1888, 1970, 2924
Hpy8I GTNNAC 6 cut(s) 782, 1063, 1079, 1888, 1970, 2924
HpyCH4III ACNGT 5 cut(s) 987, 1076, 1504, 1722, 3047
HpyCH4IV ACGT 3 cut(s) 1114, 1826, 2418
HpyF3I CTNAG 4 cut(s) 110, 1892, 2360, 3125
HpySE526I ACGT 3 cut(s) 1114, 1826, 2418
Hsp92I GRCGYC 1 cut(s) 2178
HspAI GCGC 4 cut(s) 1460, 1603, 1684, 2761
KpnI GGTACC 1 cut(s) 2354
Ksp22I TGATCA 2 cut(s) 643, 1296
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 539, 850, 1378, 1903, 2145
LweI GCATC 9 cut(s) 220, 349, 730, 1117, 2077, 2764, 2803, 3251, 3333
MaeII ACGT 3 cut(s) 1114, 1826, 2418
MaeIII GTNAC 7 cut(s) 58, 353, 673, 901, 2237, 2635, 3041
MfeI CAATTG 2 cut(s) 196, 573
MflI RGATCY 2 cut(s) 1404, 2226
MlsI TGGCCA 2 cut(s) 3111, 3307
MluNI TGGCCA 2 cut(s) 3111, 3307
MlyI GAGTC 3 cut(s) 728, 1030, 2440
MmeI TCCRAC 4 cut(s) 696, 890, 2649, 3265
Mox20I TGGCCA 2 cut(s) 3111, 3307
Mph1103I ATGCAT 2 cut(s) 1440, 2818
MroXI GAANNNNTTC 1 cut(s) 1628
MscI TGGCCA 2 cut(s) 3111, 3307
MseI TTAA 5 cut(s) 242, 651, 1466, 1674, 2294
MslI CAYNNNNRTG 1 cut(s) 969
Msp20I TGGCCA 2 cut(s) 3111, 3307
MspCI CTTAAG 1 cut(s) 1673
MspI CCGG 2 cut(s) 2472, 2633
MspR9I CCNGG 7 cut(s) 101, 253, 1867, 2072, 2472, 2633, 3142
MunI CAATTG 2 cut(s) 196, 573
Mva1269I GAATGC 3 cut(s) 77, 1319, 1440
MvaI CCWGG 5 cut(s) 101, 253, 1867, 2072, 3142
MvnI CGCG 1 cut(s) 248
NciI CCSGG 2 cut(s) 2472, 2633
NcoI CCATGG 1 cut(s) 3371
NlaIV GGNNCC 5 cut(s) 541, 852, 2054, 2352, 3139
NmeAIII GCCGAG 1 cut(s) 1388
NmuCI GTSAC 5 cut(s) 58, 353, 673, 2237, 2635
NsbI TGCGCA 3 cut(s) 1604, 1685, 2762
NsiI ATGCAT 2 cut(s) 1440, 2818
NspI RCATGY 2 cut(s) 1684, 2820
NspV TTCGAA 3 cut(s) 183, 996, 1550
PaeI GCATGC 1 cut(s) 2820
PagI TCATGA 2 cut(s) 640, 1333
PasI CCCWGGG 1 cut(s) 3141
PceI AGGCCT 1 cut(s) 3225
PciSI GCTCTTC 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 2 cut(s) 615, 3003
PctI GAATGC 3 cut(s) 77, 1319, 1440
PdmI GAANNNNTTC 1 cut(s) 1628
PfeI GAWTC 7 cut(s) 104, 347, 980, 1119, 1796, 2311, 2516
PflMI CCANNNNNTGG 1 cut(s) 1456
PleI GAGTC 3 cut(s) 727, 1029, 2439
PpsI GAGTC 3 cut(s) 727, 1029, 2439
Ppu21I YACGTR 1 cut(s) 1115
PshAI GACNNNNGTC 1 cut(s) 2594
PshBI ATTAAT 1 cut(s) 1466
PsiI TTATAA 1 cut(s) 1212
Psp6I CCWGG 5 cut(s) 99, 251, 1865, 2070, 3140
PspFI CCCAGC 2 cut(s) 2927, 3082
PspGI CCWGG 5 cut(s) 99, 251, 1865, 2070, 3140
PspN4I GGNNCC 5 cut(s) 541, 852, 2054, 2352, 3139
PspPI GGNCC 3 cut(s) 2540, 2916, 3137
PsrI GAACNNNNNNTAC 2 cut(s) 2024, 2056
PstI CTGCAG 2 cut(s) 2205, 2703
PstNI CAGNNNCTG 2 cut(s) 2250, 2734
PsuI RGATCY 2 cut(s) 1404, 2226
RseI CAYNNNNRTG 1 cut(s) 969
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 5 cut(s) 242, 651, 1466, 1674, 2294
Sau96I GGNCC 3 cut(s) 2540, 2916, 3137
ScaI AGTACT 2 cut(s) 1188, 3150
SchI GAGTC 3 cut(s) 728, 1030, 2440
ScrFI CCNGG 7 cut(s) 101, 253, 1867, 2072, 2472, 2633, 3142
SfaNI GCATC 9 cut(s) 220, 349, 730, 1117, 2077, 2764, 2803, 3251, 3333
SfcI CTRYAG 5 cut(s) 409, 952, 2201, 2242, 2699
SfuI TTCGAA 3 cut(s) 183, 996, 1550
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 5 cut(s) 203, 358, 1673, 2375, 2428
SmoI CTYRAG 5 cut(s) 203, 358, 1673, 2375, 2428
SpeI ACTAGT 1 cut(s) 905
SphI GCATGC 1 cut(s) 2820
SseBI AGGCCT 1 cut(s) 3225
SsiI CCGC 4 cut(s) 1025, 1412, 2745, 3396
SspI AATATT 1 cut(s) 1662
StuI AGGCCT 1 cut(s) 3225
StyD4I CCNGG 7 cut(s) 99, 251, 1865, 2070, 2470, 2631, 3140
StyI CCWWGG 6 cut(s) 431, 1847, 2871, 3169, 3256, 3371
TaaI ACNGT 5 cut(s) 987, 1076, 1504, 1722, 3047
TaiI ACGT 3 cut(s) 1117, 1829, 2421
TaqII GACCGA 1 cut(s) 786
TatI WGTACW 4 cut(s) 1186, 1677, 2602, 3148
TauI GCSGC 1 cut(s) 2748
TfiI GAWTC 7 cut(s) 104, 347, 980, 1119, 1796, 2311, 2516
Tru1I TTAA 5 cut(s) 242, 651, 1466, 1674, 2294
Tru9I TTAA 5 cut(s) 242, 651, 1466, 1674, 2294
TseFI GTSAC 5 cut(s) 58, 353, 673, 2237, 2635
Tsp45I GTSAC 5 cut(s) 58, 353, 673, 2237, 2635
TspGWI ACGGA 5 cut(s) 80, 2292, 2360, 2939, 3029
Van91I CCANNNNNTGG 1 cut(s) 1456
Vha464I CTTAAG 1 cut(s) 1673
VspI ATTAAT 1 cut(s) 1466
XagI CCTNNNNNAGG 1 cut(s) 3072
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 2 cut(s) 1684, 2820
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 2 cut(s) 1969, 2923
XmnI GAANNNNTTC 1 cut(s) 1628
ZrmI AGTACT 2 cut(s) 1188, 3150
Zsp2I ATGCAT 2 cut(s) 1440, 2818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.