RLG00000019518

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005191
Physical Location & Seq
Forward (+)
53036550 .. 53040624
4075 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019518

Sequence Viewer

Length: 3315 bp
ATGGCCGAAATAGCAACTCACGTTGTGGGGATTGCCATCACTAAATTGTCTCCTCTTGTCACCACGGAGTTTCTTGCATTCACTAATGTCAAGGATGACCTGGAATCCCTCAGAAGCACTCTAACAGATGTTCGGGAGTTGCTTGGAATTCTGGAAAGAAACCGGACGAATTCTTCAGGTTTCGGACAGATGAACAATTGGCTCAAGAAGCTTCAAGTAGCAGCTTATGATGCAGGGGATTTAATCGAGTCCTGGGCAGTAGAATATCACCAATGGAAGACGAAGAAGCAGGTACGAAAATTCTCTCTTCCATTTAGTGCTTCAAAATTTTTCTTTCAGCATCAAGAATCATGTGACTTGAGAGAAATTACAGCGAGAATAAATAAGATTTTACAAGATGGACAAGTCTATAGAACAATCATTGGAGTGCCTAGGAGTGAAAGCCTTTCACGGCAAACTGGCTCTCTTTCTAGCAATATAATTGAGGGCAGGGGAGATGATGTAGAGAACATAATAAAACTGCTGATACCAGATGAAGGAGCCAGTGATGAGGGCAGTATTGCTTTTATTCCAATTGTGGGGATGGGGGGCTTGGGTAAAACAACTCTCGCTCAACTCGTATATAAGAATGGCAGGGTTCATGATCATTTTAAAAGAAAATTCTGGGTTTGTGTCACAGAAACTTATGACATGACTAGAATTCTCAAAGGAATGTTGGAGTCTAATGAGATTACTTTTGATGCCAATATCACATTCGAGGTACTGCAAGACCGAGTTCATGAACTCGTGGCTGAAAAACGATTTTTACTTGTTCTAGATGATTTATGGAACAATAATTATATGGATTTGGAGCCACTAGAAAATTTACTGAAACAAGGGGTCAGTGGAAGTAAGGTCTTGGTTACTAGTCGGAACAATGAAGTTCGGAAGATTACGGGTGCAAAATCCTCCTATAGCTTGCGACATTTCAATGATGATGAATCGTGGTCGTTATTCAAAAAAATTGCCTTTGAAGGAAGGAGTCCGCCAGATGAGTTGAAGGAATATGCAAAGCAAATTGTAAACAAGTGCAACGGTTTACCTCTGGCAGTGAAACAAATGGGGGGTCTGCTACGTGGAATCACTGATGAAGAAGAGTGGAGATATATCGCTAAAAGTGAAATATGGGAACTAGAAAAAGATAAGGTACTGCCTGCTCTTCGATTGAGTTATAATCATCTGTCTTCAGCTCTGAAGCAATGTTTTGCGTATTGTTCCCTGTTTCCAAAAGCTCATGTTTTTGATAAAAATGAGTTGGTCAAATTATGGATGGCAGAAGCATTCATTCAACCTCATGACAGACACAGGACAGAAGACATAGGAAGCAGATACTTCAAGGAGCTGTCAGACAGGTTCTTCTTTGAGATCTCGGCGGAAGACAAGAGCAAGTGTAGAATGCATGATCTTATCCACGACTTGGCGCTATTAATCTCCTCTCCCATTTTCTGCCAAGTGAAAGATACGGTGCCAAAGAGTTTAGACGAAGTATCTCGCCATGTATCACTGCTTTCGAATGAGGTTGAGCAGCCCCTTTCAGAAATAATAAAAAAATCAAAGAAGCTGCGCACACTTTTATTGCCAGTTAAACACTTCAAAGCCTTTGGGAAAGCTCAACGTGCAATATTCCATTCACTTAAGTACATGCGCACATTAGATTTGAGTTCAAGCACACTTCAAGAACTGTCGGGCTCAATCGAGAATTTGAAGCTTTTGCACTACCTTGATTTATCAAAAACTGAAATAAAAAAGCTTCCCGATTCGATATGCAACCTCTACTCTTTGGAGACGTTGAAACTTTCAGATTGCCCTTGGCTTTTCACTTTGCCCAGGAAGCTCAAAATTCTTGTCAACCTGAGACATCTGGAGCTAGATGAAATGTTTTGGTACAAGTTATCAACGCTTCCAGAAAGCATGGGGTGTCTAACCAGTCTGCACAATCTTCACAAATTTCAGGTTGGCTGCAATACAGGATACAAGCTTCAAGAATTGAAGAACATGAAGTACCTTACAGGGTTCCTGCACATCTCGAACCTGGAAAATGCAGTGGATGCAGGGGAGGCCAGCTTGAAAGAAAAAGAAATGATTGATAAAGTGGTATATGAATGGAGCAGCAGCAATCTGAATTTGCAAGATGAAGACGCCAAGCAGGTACTCGAAAACCTGCAGCCTCACCCAGGGGTCCAAGAGATCCAAATCTGTCACTACAAGAGCTCTGAATTTCCAATTTGGATCAGATACGGAAAGTATGAGAACTTGAAGACTATATATTTGAATCATTGCACAGGGATCAAAATCCTCTCTCTTGGCGAGTTACCCAACCTCAGAGAACTCCGTCTCAAGAACATGGCAGAAATGATCGAATGGAAGGAAGAGAAGAACCCGTTCCTCTTGAGTCTTAAGATAAGTGGCTGCCCAAAGCTGACAAAACTGCCCAGCTTCTTTACTGCATTACATAGTATGAAGATCAAGAATTGTGAATCATTGGATACCCTTCCAGTTGGCCCAGTGCGACTCATAAGACTTGCTGGCAATCCTGTGCTGAAGCACTGGACTGAAGTGAATGTAATAATACCGACAATTGTCGATGGTCGCCCAGTCAAAATTCATGGCATGACAAGTCATTTTCTGTTGGAAGCAAACATTATCAACTGCCCTGAGCTGCAGACGTTGCCAAGAGGACTGCATCCACAGAAACTGGAAATCAGTGGCTGCAAGAGCTTGAGCACCCTACCAGACGCAGACCATGCAGTACGGCTTGGGGTTTTAGCATTGGATGCATGCCGTGATGAAACATTAGTCGGTATGATCCCCAGTTCTGGGACTTTATATTCCTTGGTGATTTCGAACATCCCAAATCTCATTTGTATTCCAAAGTGGCCCAGTCTCCCCAGCCTCCAAGCATTGTATATCCGTGATTGCGCAGAACTTGAATATCTATCCAACCAAGAGAACGGGGACGGGTTGTTCGACGGCTTCAACTCTCTAAAGCATCTCTCCATCCGTAACTGTCCTAAACTTCTGACACTGCCAGCTGAAGGTCTCCCAACTAGTCTTCAAGTTTTGAGTGTTGGCCCATGTGCAAGTCTCAGTTCGTTTGGCCCTAGGGAAGTACTCGCAAAACTCACATCCCTTGGTGATCTCTACCTTGAAGGTACCGCAGAGGACACAAGACCAAAGGAAAGAAGGCATTGGAATGATGCCAAGCTTCTCGAATTGCACAAAAGGGAGGTCAAGTTGGATGGAAGACTCGATGTTGGCCAAGTAAAACGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1105

Amino Acids

125.46

Weight (kDa)

7.74

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 100 3.9e-13 Rx N-terminal domain
NB-ARC PF00931 168 - 340 6.6e-32 NB-ARC domain
WHD_DRP PF23559 420 - 487 1.9e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 544 - 801 2e-22 Leucine-rich repeat region
LRR_8 PF13855 561 - 615 1e-05 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 673 - 795 6e-27 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1212
Acc16I TGCGCA 3 cut(s) 1604, 1685, 2959
Acc36I ACCTGC 3 cut(s) 280, 2176, 2208
Acc65I GGTACC 1 cut(s) 3191
AccB1I GGYRCC 2 cut(s) 1504, 3191
AccB7I CCANNNNNTGG 1 cut(s) 1456
AciI CCGC 3 cut(s) 1025, 1412, 3195
AclWI GGATC 4 cut(s) 2221, 2276, 2333, 2839
AcoI YGGCCR 2 cut(s) 3, 3295
AcuI CTGAAG 6 cut(s) 159, 1209, 1253, 2600, 2613, 3093
AcyI GRCGYC 1 cut(s) 2178
AdeI CACNNNGTG 1 cut(s) 25
AfiI CCNNNNNNNGG 7 cut(s) 536, 578, 1456, 2213, 2855, 2856, 3074
AflII CTTAAG 2 cut(s) 1673, 2435
AhlI ACTAGT 2 cut(s) 905, 3086
AjnI CCWGG 5 cut(s) 99, 251, 1865, 2070, 2212
AjuI GAANNNNNNNTTGG 2 cut(s) 737, 769
AloI GAACNNNNNNTCC 2 cut(s) 2987, 3019
Alw21I GWGCWC 2 cut(s) 2252, 2765
Alw26I GTCTC 7 cut(s) 54, 1817, 1888, 2378, 2927, 3083, 3128
AlwI GGATC 4 cut(s) 2221, 2276, 2333, 2839
AlwNI CAGNNNCTG 2 cut(s) 2734, 2748
AoxI GGCC 7 cut(s) 3, 2097, 2539, 2915, 3109, 3136, 3295
AseI ATTAAT 1 cut(s) 1466
Asp700I GAANNNNTTC 1 cut(s) 2420
Asp718I GGTACC 1 cut(s) 3191
AspA2I CCTAGG 2 cut(s) 431, 3140
AspLEI GCGC 4 cut(s) 1462, 1605, 1686, 2960
AspS9I GGNCC 5 cut(s) 2218, 2540, 2916, 3110, 3137
AsuHPI GGTGA 5 cut(s) 52, 260, 2201, 2887, 3185
AsuII TTCGAA 2 cut(s) 1550, 2882
AvaII GGWCC 1 cut(s) 2218
AvrII CCTAGG 2 cut(s) 431, 3140
BalI TGGCCA 1 cut(s) 3297
BanI GGYRCC 2 cut(s) 1504, 3191
BanII GRGCYC 2 cut(s) 1730, 2252
BauI CACGAG 1 cut(s) 785
BbsI GAAGAC 8 cut(s) 284, 1215, 1359, 1422, 2181, 2303, 3083, 3289
Bbv12I GWGCWC 2 cut(s) 2252, 2765
BccI CCATC 7 cut(s) 44, 392, 577, 1303, 2618, 3044, 3272
BceAI ACGGC 4 cut(s) 467, 2805, 2807, 3025
BciT130I CCWGG 5 cut(s) 101, 253, 1867, 2072, 2214
BciVI GTATCC 2 cut(s) 2003, 2518
BclI TGATCA 1 cut(s) 643
BcoDI GTCTC 7 cut(s) 54, 1817, 1888, 2378, 2927, 3083, 3128
BcuI ACTAGT 2 cut(s) 905, 3086
BfmI CTRYAG 4 cut(s) 409, 952, 2201, 2699
BfoI RGCGCY 1 cut(s) 1463
BfrI CTTAAG 2 cut(s) 1673, 2435
BfuAI ACCTGC 3 cut(s) 280, 2176, 2208
BfuI GTATCC 2 cut(s) 2003, 2518
BglII AGATCT 1 cut(s) 1404
BlnI CCTAGG 2 cut(s) 431, 3140
BmcAI AGTACT 1 cut(s) 3150
Bme1390I CCNGG 5 cut(s) 101, 253, 1867, 2072, 2214
Bme18I GGWCC 1 cut(s) 2218
BmgT120I GGNCC 5 cut(s) 2218, 2540, 2916, 3110, 3137
BmiI GGNNCC 6 cut(s) 541, 852, 1506, 2054, 2219, 3193
BmrFI CCNGG 5 cut(s) 101, 253, 1867, 2072, 2214
BmrI ACTGGG 4 cut(s) 2537, 2627, 2844, 2913
BmsI GCATC 8 cut(s) 220, 349, 730, 2077, 2731, 2803, 3037, 3226
BmuI ACTGGG 4 cut(s) 2537, 2627, 2844, 2913
BoxI GACNNNNGTC 1 cut(s) 2618
BpiI GAAGAC 8 cut(s) 284, 1215, 1359, 1422, 2181, 2303, 3083, 3289
BpmI CTGGAG 1 cut(s) 1922
Bpu10I CCTNAGC 1 cut(s) 2694
Bpu14I TTCGAA 2 cut(s) 1550, 2882
BpuEI CTTGAG 5 cut(s) 188, 379, 2360, 2449, 2779
BsaAI YACGTR 1 cut(s) 1115
BsaBI GATNNNNATC 3 cut(s) 35, 2231, 2330
BsaHI GRCGYC 1 cut(s) 2178
BsaI GGTCTC 1 cut(s) 3083
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 486, 516, 3257, 3287
Bsc4I CCNNNNNNNGG 7 cut(s) 536, 578, 1456, 2213, 2855, 2856, 3074
Bse3DI GCAATG 2 cut(s) 1244, 2314
Bse8I GATNNNNATC 3 cut(s) 35, 2231, 2330
BseBI CCWGG 5 cut(s) 101, 253, 1867, 2072, 2214
BseJI GATNNNNATC 3 cut(s) 35, 2231, 2330
BseLI CCNNNNNNNGG 7 cut(s) 536, 578, 1456, 2213, 2855, 2856, 3074
BseMI GCAATG 2 cut(s) 1244, 2314
BseMII CTCAG 5 cut(s) 124, 1883, 2374, 2685, 3139
BseRI GAGGAG 2 cut(s) 42, 1462
BseYI CCCAGC 2 cut(s) 2471, 2927
BsgI GTGCAG 2 cut(s) 1955, 2042
BshFI GGCC 7 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3297
BshNI GGYRCC 2 cut(s) 1504, 3191
BsiHKAI GWGCWC 2 cut(s) 2252, 2765
BsiSI CCGG 1 cut(s) 163
BslFI GGGAC 2 cut(s) 2872, 3008
BslI CCNNNNNNNGG 7 cut(s) 536, 578, 1456, 2213, 2855, 2856, 3074
BsmAI GTCTC 7 cut(s) 54, 1817, 1888, 2378, 2927, 3083, 3128
BsmBI CGTCTC 2 cut(s) 1817, 2378
BsmFI GGGAC 2 cut(s) 2872, 3008
BsmI GAATGC 3 cut(s) 77, 1319, 1440
BsnI GGCC 7 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3297
Bso31I GGTCTC 1 cut(s) 3083
Bsp119I TTCGAA 2 cut(s) 1550, 2882
Bsp1286I GDGCHC 3 cut(s) 1730, 2252, 2765
BspACI CCGC 3 cut(s) 1025, 1412, 3195
BspANI GGCC 7 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3297
BspCNI CTCAG 5 cut(s) 123, 1884, 2373, 2686, 3138
BspHI TCATGA 3 cut(s) 640, 778, 1333
BspLI GGNNCC 6 cut(s) 541, 852, 1506, 2054, 2219, 3193
BspMAI CTGCAG 2 cut(s) 2205, 2703
BspMI ACCTGC 3 cut(s) 280, 2176, 2208
BspPI GGATC 4 cut(s) 2221, 2276, 2333, 2839
BspQI GCTCTTC 1 cut(s) 1203
BspT104I TTCGAA 2 cut(s) 1550, 2882
BspT107I GGYRCC 2 cut(s) 1504, 3191
BspTI CTTAAG 2 cut(s) 1673, 2435
BspTNI GGTCTC 1 cut(s) 3083
BsrDI GCAATG 2 cut(s) 1244, 2314
BssNI GRCGYC 1 cut(s) 2178
BssSI CACGAG 1 cut(s) 785
BssT1I CCWWGG 5 cut(s) 431, 1847, 2871, 3140, 3169
Bst2BI CACGAG 1 cut(s) 785
Bst2UI CCWGG 5 cut(s) 101, 253, 1867, 2072, 2214
Bst4CI ACNGT 4 cut(s) 1076, 1504, 1722, 3047
Bst6I CTCTTC 4 cut(s) 312, 1128, 1203, 2403
BstACI GRCGYC 1 cut(s) 2178
BstAFI CTTAAG 2 cut(s) 1673, 2435
BstAPI GCANNNNNTGC 4 cut(s) 2087, 2707, 2783, 2813
BstBAI YACGTR 1 cut(s) 1115
BstBI TTCGAA 2 cut(s) 1550, 2882
BstC8I GCNNGC 6 cut(s) 959, 1194, 2101, 2566, 2818, 3069
BstDEI CTNAG 5 cut(s) 110, 1892, 2360, 2694, 3125
BstDSI CCRYGG 1 cut(s) 63
BstENI CCTNNNNNAGG 1 cut(s) 2211
BstH2I RGCGCY 1 cut(s) 1463
BstHHI GCGC 4 cut(s) 1462, 1605, 1686, 2960
BstMAI GTCTC 7 cut(s) 54, 1817, 1888, 2378, 2927, 3083, 3128
BstNI CCWGG 5 cut(s) 101, 253, 1867, 2072, 2214
BstNSI RCATGY 2 cut(s) 1684, 2820
BstPAI GACNNNNGTC 1 cut(s) 2618
BstSCI CCNGG 5 cut(s) 99, 251, 1865, 2070, 2212
BstSFI CTRYAG 4 cut(s) 409, 952, 2201, 2699
BstV2I GAAGAC 8 cut(s) 284, 1215, 1359, 1422, 2181, 2303, 3083, 3289
BstX2I RGATCY 2 cut(s) 1404, 2226
BstYI RGATCY 2 cut(s) 1404, 2226
BsuI GTATCC 2 cut(s) 2003, 2518
BsuRI GGCC 7 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3297
BtgI CCRYGG 1 cut(s) 63
BtsI GCAGTG 4 cut(s) 1095, 1541, 2088, 3062
BveI ACCTGC 3 cut(s) 280, 2176, 2208
Cac8I GCNNGC 6 cut(s) 959, 1194, 2101, 2566, 2818, 3069
CaiI CAGNNNCTG 2 cut(s) 2734, 2748
CciI TCATGA 3 cut(s) 640, 778, 1333
CfoI GCGC 4 cut(s) 1462, 1605, 1686, 2960
Cfr13I GGNCC 5 cut(s) 2218, 2540, 2916, 3110, 3137
CseI GACGC 2 cut(s) 2186, 2783
DdeI CTNAG 5 cut(s) 110, 1892, 2360, 2694, 3125
DraI TTTAAA 1 cut(s) 652
DraIII CACNNNGTG 1 cut(s) 25
EaeI YGGCCR 2 cut(s) 3, 3295
Eam1104I CTCTTC 4 cut(s) 312, 1128, 1203, 2403
EarI CTCTTC 4 cut(s) 312, 1128, 1203, 2403
EciI GGCGGA 2 cut(s) 1014, 1427
Ecl136II GAGCTC 1 cut(s) 2250
Eco130I CCWWGG 5 cut(s) 431, 1847, 2871, 3140, 3169
Eco24I GRGCYC 2 cut(s) 1730, 2252
Eco31I GGTCTC 1 cut(s) 3083
Eco47I GGWCC 1 cut(s) 2218
Eco53kI GAGCTC 1 cut(s) 2250
Eco57I CTGAAG 6 cut(s) 159, 1209, 1253, 2600, 2613, 3093
EcoICRI GAGCTC 1 cut(s) 2250
EcoNI CCTNNNNNAGG 1 cut(s) 2211
EcoRI GAATTC 3 cut(s) 147, 169, 699
EcoRII CCWGG 5 cut(s) 99, 251, 1865, 2070, 2212
EcoT14I CCWWGG 5 cut(s) 431, 1847, 2871, 3140, 3169
EcoT22I ATGCAT 2 cut(s) 1440, 2818
EcoT38I GRGCYC 2 cut(s) 1730, 2252
ErhI CCWWGG 5 cut(s) 431, 1847, 2871, 3140, 3169
Esp3I CGTCTC 2 cut(s) 1817, 2378
FaqI GGGAC 2 cut(s) 2872, 3008
FbaI TGATCA 1 cut(s) 643
FriOI GRGCYC 2 cut(s) 1730, 2252
FspAI RTGCGCAY 1 cut(s) 1685
FspI TGCGCA 3 cut(s) 1604, 1685, 2959
GlaI GCGC 4 cut(s) 1461, 1604, 1685, 2959
GsaI CCCAGC 2 cut(s) 2475, 2931
GsuI CTGGAG 1 cut(s) 1922
HaeII RGCGCY 1 cut(s) 1463
HaeIII GGCC 7 cut(s) 5, 2099, 2541, 2917, 3111, 3138, 3297
HapII CCGG 1 cut(s) 163
HgaI GACGC 2 cut(s) 2186, 2783
HhaI GCGC 4 cut(s) 1462, 1605, 1686, 2960
Hin1I GRCGYC 1 cut(s) 2178
Hin6I GCGC 4 cut(s) 1460, 1603, 1684, 2958
HinP1I GCGC 4 cut(s) 1460, 1603, 1684, 2958
HincII GTYRAC 1 cut(s) 1888
HindII GTYRAC 1 cut(s) 1888
HindIII AAGCTT 5 cut(s) 209, 1745, 1787, 2015, 3242
HpaII CCGG 1 cut(s) 163
HphI GGTGA 5 cut(s) 52, 260, 2201, 2887, 3185
Hpy166II GTNNAC 3 cut(s) 1063, 1079, 1888
Hpy8I GTNNAC 3 cut(s) 1063, 1079, 1888
Hpy99I CGWCG 1 cut(s) 3011
HpyAV CCTTC 9 cut(s) 530, 1007, 1011, 1033, 2398, 2540, 3068, 3182, 3216
HpyCH4III ACNGT 4 cut(s) 1076, 1504, 1722, 3047
HpyCH4IV ACGT 5 cut(s) 21, 1114, 1654, 1826, 2705
HpyF3I CTNAG 5 cut(s) 110, 1892, 2360, 2694, 3125
HpySE526I ACGT 5 cut(s) 21, 1114, 1654, 1826, 2705
Hsp92I GRCGYC 1 cut(s) 2178
HspAI GCGC 4 cut(s) 1460, 1603, 1684, 2958
KpnI GGTACC 1 cut(s) 3195
Ksp22I TGATCA 1 cut(s) 643
LguI GCTCTTC 1 cut(s) 1203
LmnI GCTCC 5 cut(s) 539, 850, 1378, 1903, 2145
LweI GCATC 8 cut(s) 220, 349, 730, 2077, 2731, 2803, 3037, 3226
MaeII ACGT 5 cut(s) 21, 1114, 1654, 1826, 2705
MaeIII GTNAC 7 cut(s) 58, 353, 673, 901, 2237, 2349, 3041
MfeI CAATTG 3 cut(s) 196, 573, 2616
MflI RGATCY 2 cut(s) 1404, 2226
MhlI GDGCHC 3 cut(s) 1730, 2252, 2765
MlsI TGGCCA 1 cut(s) 3297
MluNI TGGCCA 1 cut(s) 3297
MlyI GAGTC 6 cut(s) 257, 728, 1030, 2440, 2544, 3279
MmeI TCCRAC 5 cut(s) 696, 890, 2649, 3003, 3255
Mox20I TGGCCA 1 cut(s) 3297
Mph1103I ATGCAT 2 cut(s) 1440, 2818
MroXI GAANNNNTTC 1 cut(s) 2420
MscI TGGCCA 1 cut(s) 3297
MseI TTAA 7 cut(s) 242, 651, 1466, 1623, 1674, 2436, 3313
MslI CAYNNNNRTG 3 cut(s) 425, 969, 3231
Msp20I TGGCCA 1 cut(s) 3297
MspA1I CMGCKG 1 cut(s) 3071
MspCI CTTAAG 2 cut(s) 1673, 2435
MspI CCGG 1 cut(s) 163
MspR9I CCNGG 5 cut(s) 101, 253, 1867, 2072, 2214
MunI CAATTG 3 cut(s) 196, 573, 2616
Mva1269I GAATGC 3 cut(s) 77, 1319, 1440
MvaI CCWGG 5 cut(s) 101, 253, 1867, 2072, 2214
NlaIV GGNNCC 6 cut(s) 541, 852, 1506, 2054, 2219, 3193
NmeAIII GCCGAG 1 cut(s) 1388
NmuCI GTSAC 4 cut(s) 58, 353, 673, 2237
NsbI TGCGCA 3 cut(s) 1604, 1685, 2959
NsiI ATGCAT 2 cut(s) 1440, 2818
NspI RCATGY 2 cut(s) 1684, 2820
NspV TTCGAA 2 cut(s) 1550, 2882
PaeI GCATGC 1 cut(s) 2820
PagI TCATGA 3 cut(s) 640, 778, 1333
PasI CCCWGGG 1 cut(s) 2213
PciSI GCTCTTC 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 2 cut(s) 615, 3003
PctI GAATGC 3 cut(s) 77, 1319, 1440
PdmI GAANNNNTTC 1 cut(s) 2420
PfeI GAWTC 7 cut(s) 104, 347, 980, 1119, 1796, 2311, 2516
PflMI CCANNNNNTGG 1 cut(s) 1456
PleI GAGTC 6 cut(s) 256, 727, 1029, 2439, 2544, 3279
PpsI GAGTC 6 cut(s) 256, 727, 1029, 2439, 2544, 3279
Ppu21I YACGTR 1 cut(s) 1115
PshAI GACNNNNGTC 1 cut(s) 2618
PshBI ATTAAT 1 cut(s) 1466
PsiI TTATAA 1 cut(s) 1212
Psp124BI GAGCTC 1 cut(s) 2252
Psp6I CCWGG 5 cut(s) 99, 251, 1865, 2070, 2212
PspFI CCCAGC 2 cut(s) 2471, 2927
PspGI CCWGG 5 cut(s) 99, 251, 1865, 2070, 2212
PspN4I GGNNCC 6 cut(s) 541, 852, 1506, 2054, 2219, 3193
PspPI GGNCC 5 cut(s) 2218, 2540, 2916, 3110, 3137
PsrI GAACNNNNNNTAC 2 cut(s) 2024, 2056
PstI CTGCAG 2 cut(s) 2205, 2703
PstNI CAGNNNCTG 2 cut(s) 2734, 2748
PsuI RGATCY 2 cut(s) 1404, 2226
PvuII CAGCTG 1 cut(s) 3071
RseI CAYNNNNRTG 3 cut(s) 425, 969, 3231
SacI GAGCTC 1 cut(s) 2252
SapI GCTCTTC 1 cut(s) 1203
SaqAI TTAA 7 cut(s) 242, 651, 1466, 1623, 1674, 2436, 3313
Sau96I GGNCC 5 cut(s) 2218, 2540, 2916, 3110, 3137
ScaI AGTACT 1 cut(s) 3150
SchI GAGTC 6 cut(s) 257, 728, 1030, 2440, 2544, 3279
ScrFI CCNGG 5 cut(s) 101, 253, 1867, 2072, 2214
SduI GDGCHC 3 cut(s) 1730, 2252, 2765
SfaNI GCATC 8 cut(s) 220, 349, 730, 2077, 2731, 2803, 3037, 3226
SfcI CTRYAG 4 cut(s) 409, 952, 2201, 2699
SfuI TTCGAA 2 cut(s) 1550, 2882
SinI GGWCC 1 cut(s) 2218
SmiMI CAYNNNNRTG 3 cut(s) 425, 969, 3231
SmlI CTYRAG 7 cut(s) 203, 358, 1673, 2375, 2428, 2435, 2758
SmoI CTYRAG 7 cut(s) 203, 358, 1673, 2375, 2428, 2435, 2758
SpeI ACTAGT 2 cut(s) 905, 3086
SphI GCATGC 1 cut(s) 2820
SsiI CCGC 3 cut(s) 1025, 1412, 3195
SspI AATATT 1 cut(s) 1662
SstI GAGCTC 1 cut(s) 2252
StyD4I CCNGG 5 cut(s) 99, 251, 1865, 2070, 2212
StyI CCWWGG 5 cut(s) 431, 1847, 2871, 3140, 3169
TaaI ACNGT 4 cut(s) 1076, 1504, 1722, 3047
TaiI ACGT 5 cut(s) 24, 1117, 1657, 1829, 2708
TaqII GACCGA 1 cut(s) 786
TatI WGTACW 2 cut(s) 1677, 3148
TfiI GAWTC 7 cut(s) 104, 347, 980, 1119, 1796, 2311, 2516
Tru1I TTAA 7 cut(s) 242, 651, 1466, 1623, 1674, 2436, 3313
Tru9I TTAA 7 cut(s) 242, 651, 1466, 1623, 1674, 2436, 3313
TseFI GTSAC 4 cut(s) 58, 353, 673, 2237
Tsp45I GTSAC 4 cut(s) 58, 353, 673, 2237
TspGWI ACGGA 5 cut(s) 80, 2292, 2360, 2939, 3029
Van91I CCANNNNNTGG 1 cut(s) 1456
Vha464I CTTAAG 2 cut(s) 1673, 2435
VpaK11BI GGWCC 1 cut(s) 2218
VspI ATTAAT 1 cut(s) 1466
XagI CCTNNNNNAGG 1 cut(s) 2211
XbaI TCTAGA 1 cut(s) 814
XceI RCATGY 2 cut(s) 1684, 2820
XmaJI CCTAGG 2 cut(s) 431, 3140
XmnI GAANNNNTTC 1 cut(s) 2420
ZrmI AGTACT 1 cut(s) 3150
Zsp2I ATGCAT 2 cut(s) 1440, 2818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.