RchiOBHm_Chr2g0136171

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005191
Physical Location & Seq
Forward (+)
53475012 .. 53477602
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50699

Sequence Viewer

Length: 2544 bp
ATGGCTTCAGTAGTTATTCCCCTCGCAACTCCTGTTGTGCGGCTTGCACTAACTAAATTGACCTCTTTTGTCACCACCGAGGTTTCTGCATTCAATCATGTCAAGGATGAAATAGAAACGCTGAAGAACACTTTAACAGAAATTCAAGCTGTGCTTACAAGGTTGGAGGCAGTTCAGCAGAGTTCTTCCATGGAGTGGTTCAACAGGCTTCGAGTAGCTGCTTATGATGCAGATGATCTAATTTACTCCTGGGCGACAAGGTATCTCCAATGGAGCATGAAGAAGGAGGTACAAATAATCCATCTTCCCTTTAGTACTTCAAACTTTCTGTTTCAGTATCTTCAATCCCGTAAGTTGAGAAAGATTATAGCGACACTAAATAAGATTTTGGAAGATGGACAACGCTATAAAAACATTACTGACACTCCCATGCCTACAACTGGAAATCCTTCACAATCACAGCAAACTGGCTCTGTTTCAAGCAGTTTAGTTGTTAGCAGGGAAGAAGATAAAGAAAAGATAATAAGACTGCTGACAACGGATGAAGAATCCAGTGACGGGCAAAACATTTCTTTTATTCCCATTGTAGGCATGGGAGGTTTGGGGAAAACAACTCTTGCTCAGCTTGTATATAACGATGACAGGATGAATCAGACTTTCGAAACTAAAATATGGGTTTGTGTGACGGACAATTTTGACATCAAGAGGATTTTCAGAGAAATAGTGATGTCTCAAATGTCTAAAAAAATAATTGGCCATGATGCCAACTCTATCTCCGTCGACAAACTAGAAACCCATGCTAAGGATATCCTGGCACAAAAAAGGTTTTTACTTGTCCTAGATGATTTATGGACGTACAACCATGTGGATTTGGAGCCCCTTGAAAAAATCTTAGTTCAAGGCGGCAGGGGAAGCAAGGTTCTGGTGACTAGTAGGATCTCAGAAATTTCAAATCTCAAGTATTCAAAATCCGCTTATTTTCTGCAATGTTTGGGTGATACTGAATCATGGTCGTTGTTTCAAAACATAGCATTCAAAGAACACCTTTTGACAGGTCGTGTACGTGAGGAGTTGGAAGTGTATGGAAAAGAAATTGTGAGCAAGTGCAAAGGTTTACCTCTGGCAGTGAAACAAATGGGAGGTCTGCTATGTGGGAATACTGATGTAAATCGATGGAAGAATATCAAGTCAAGTCAAACATGGGAAGTAGGGGATGATAGAGTACTGCCTGCTCTTAGATTGAGTTACGATCATCTGCTACCAAATCACAAGCAGTGCTTCGCATACTGTTCCTTGTTCCCAAAGGCTTATGTTTATGAAAAGGATGAATTGGTCAAACTATGGATGGCAGAAGCATTAATTGAGCCTAATAATAGAGAAAGAACAGAAGTCATCGGAAGCACATACTTGAAGGAGCTGTCAAACAGGTTCTTTTTTGAGCGCGAAGATGAGACAAAGTATAGGATGCATGATGTTATCCATGACTTGGCACAAGAGATCTCCAGTCCCTTCTGTTGCCAAGTGAAGGATAAGAAAGATATTATGACACCGAATTTCAATGAAAAATCTCGCCATGTATCACTGCTTTCAGAAGAAATTGAGCAACCTACTTTGGAGATAATCAAAAAGTCAAAGAAGCTGCGGACACTTATATTGCCTGTTCAACACTTGAAAGCCTTTGGGAAAGTGCAGCATGAAATATTCCATTCACTGAGGTATATGCGCACCTTAGACCTGAGTTCAAGCACAATTCAAACACTCCCAGAGTCTATTGAAAAGCTGAGGCTTTTGCACTATCTTGATTTATCAAAAACTGAAATCACAGCACTTCCCGACTCAATATGCAAACTGTACAATTTGCAGACGTTGAAACTTCTGCAGTGCCCTTGGCTTTTCAATTTGCCCACAAACCTCAAAACTCTTGTCAATTTGAGACATCTGGAGCTAGATGAAATGTTCTGGTATAAGGCTTCGATGCTTCCACGAAGCATTGGGTGTCTAACCAGTCTGCACAATTTACACAAATTTCAGGTTAGCTTTTCTTTTACATTTGTTGTTGCCTTGCTGAACTTGGGACCTTTGACCTTGCACTGCCATCTGACTGAAAGTCTTATTAATAATAACAATAATGATGGATCTCAGGTTGGCTGCAAAACCGGATACAAACTTGAAGAATTGAAGAATATGGAGTACCTTACAGGAACGCTGCACATATCAAAGCTAGAAAATGCAACAAATGCAGAGGAGGCTAACTTGAAGGAAAAAGAATTGATTGAAAAAGTGATATATGAATGGAGCAACAGTGATCTTAATTTGCAAGATGAAGCCACTGAAAAGCAGGTACTTGAAGACATACAACCTCACTCAAGGGTCAAAGAGCTCCAAATCTGTCACTACAGGAGCACTGAATTTCCAATTTGGATGAGAGGCAGACAGCTCCAGAATTTAGTTAACATTGAGTTGAATCATTGCACAAGGATCACAATCCTCTCTCTGGTTGAAGGAATGGCAAGAAGAGACTTATCAATCCTTAGAGGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

847

Amino Acids

97.5

Weight (kDa)

6.93

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 94 8e-16 Rx N-terminal domain
NB-ARC PF00931 168 - 348 5.1e-33 NB-ARC domain
WHD_DRP PF23559 432 - 497 2.5e-21 Disease resistance protein Winged helix domain
LRR_8 PF13855 560 - 606 4.5e-06 Leucine rich repeat
LRR_14 PF23598 590 - 739 2.5e-12 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 723 - 832 1.2e-25 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1724
Acc36I ACCTGC 1 cut(s) 2329
AccB7I CCANNNNNTGG 2 cut(s) 195, 1486
AccI GTMKAC 1 cut(s) 780
AccII CGCG 1 cut(s) 1443
AciI CCGC 4 cut(s) 40, 903, 972, 1642
AclWI GGATC 3 cut(s) 944, 2143, 2486
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 6 cut(s) 141, 945, 1552, 2024, 2408, 2443
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 8 cut(s) 291, 316, 857, 1062, 1224, 1853, 2192, 2343
AfiI CCNNNNNNNGG 8 cut(s) 195, 440, 558, 587, 802, 1486, 1525, 2494
AhdI GACNNNNNGTC 1 cut(s) 2106
AhlI ACTAGT 1 cut(s) 929
AjnI CCWGG 2 cut(s) 248, 810
AloI GAACNNNNNNTCC 2 cut(s) 903, 935
Alw21I GWGCWC 2 cut(s) 2382, 2405
Alw26I GTCTC 4 cut(s) 735, 1445, 1927, 2511
AlwI GGATC 3 cut(s) 944, 2143, 2486
AoxI GGCC 1 cut(s) 754
ApeKI GCWGC 5 cut(s) 218, 1639, 1690, 2148, 2206
ApoI RAATTY 6 cut(s) 141, 945, 1552, 2024, 2408, 2443
ArsI GACNNNNNNTTYG 4 cut(s) 1126, 1158, 1908, 1940
AseI ATTAAT 2 cut(s) 1358, 2115
Asp700I GAANNNNTTC 1 cut(s) 448
AspLEI GCGC 2 cut(s) 1443, 1725
AspS9I GGNCC 1 cut(s) 2075
AsuHPI GGTGA 3 cut(s) 64, 937, 1007
AsuII TTCGAA 1 cut(s) 660
AvaII GGWCC 1 cut(s) 2075
BaeGI GKGCMC 1 cut(s) 1886
BalI TGGCCA 1 cut(s) 756
BanII GRGCYC 2 cut(s) 879, 2382
BbsI GAAGAC 1 cut(s) 2355
Bbv12I GWGCWC 2 cut(s) 2382, 2405
BbvCI CCTCAGC 1 cut(s) 1781
BbvI GCAGC 5 cut(s) 205, 1626, 1702, 2135, 2193
BccI CCATC 6 cut(s) 309, 389, 1167, 1339, 2103, 2126
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 2 cut(s) 250, 812
BciVI GTATCC 1 cut(s) 2153
BcoDI GTCTC 4 cut(s) 735, 1445, 1927, 2511
BcuI ACTAGT 1 cut(s) 929
BfaI CTAG 5 cut(s) 788, 839, 930, 1946, 2222
BfmI CTRYAG 2 cut(s) 1877, 2395
BfuAI ACCTGC 1 cut(s) 2329
BfuI GTATCC 1 cut(s) 2153
BglII AGATCT 1 cut(s) 1497
BisI GCNGC 7 cut(s) 41, 219, 904, 1640, 1691, 2149, 2207
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 7 cut(s) 42, 220, 905, 1641, 1692, 2150, 2208
BmcAI AGTACT 2 cut(s) 316, 1224
Bme1390I CCNGG 2 cut(s) 250, 812
Bme18I GGWCC 1 cut(s) 2075
BmeRI GACNNNNNGTC 1 cut(s) 2106
BmgT120I GGNCC 1 cut(s) 2075
BmiI GGNNCC 2 cut(s) 876, 2076
BmrFI CCNGG 2 cut(s) 250, 812
BmsI GCATC 4 cut(s) 217, 751, 1455, 1965
BpiI GAAGAC 1 cut(s) 2355
BpmI CTGGAG 3 cut(s) 1486, 1961, 2423
Bpu10I CCTNAGC 2 cut(s) 801, 1781
Bpu1102I GCTNAGC 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 660
BpuEI CTTGAG 2 cut(s) 941, 2350
Bsa29I ATCGAT 1 cut(s) 1171
BsaAI YACGTR 1 cut(s) 1064
BsaBI GATNNNNATC 2 cut(s) 1167, 2483
BsaJI CCNNGG 4 cut(s) 78, 189, 249, 1886
BsaWI WCCGGW 1 cut(s) 2156
BsaXI ACNNNNNCTCC 6 cut(s) 409, 439, 758, 788, 2287, 2317
Bsc4I CCNNNNNNNGG 8 cut(s) 195, 440, 558, 587, 802, 1486, 1525, 2494
Bse1I ACTGG 5 cut(s) 445, 472, 552, 1503, 2004
Bse3DI GCAATG 2 cut(s) 992, 2467
Bse8I GATNNNNATC 2 cut(s) 1167, 2483
BseBI CCWGG 2 cut(s) 250, 812
BseCI ATCGAT 1 cut(s) 1171
BseDI CCNNGG 4 cut(s) 78, 189, 249, 1886
BseGI GGATG 8 cut(s) 112, 547, 651, 1219, 1330, 1350, 1470, 2427
BseJI GATNNNNATC 2 cut(s) 1167, 2483
BseLI CCNNNNNNNGG 8 cut(s) 195, 440, 558, 587, 802, 1486, 1525, 2494
BseMI GCAATG 2 cut(s) 992, 2467
BseMII CTCAG 6 cut(s) 635, 954, 1703, 1727, 1772, 2153
BseNI ACTGG 5 cut(s) 445, 472, 552, 1503, 2004
BseRI GAGGAG 2 cut(s) 1082, 2258
BseSI GKGCMC 1 cut(s) 1886
BseXI GCAGC 5 cut(s) 205, 1626, 1702, 2135, 2193
BsgI GTGCAG 3 cut(s) 1709, 1994, 2192
Bsh1236I CGCG 1 cut(s) 1443
BshFI GGCC 1 cut(s) 756
BshVI ATCGAT 1 cut(s) 1171
BsiHKAI GWGCWC 2 cut(s) 2382, 2405
BsiSI CCGG 1 cut(s) 2157
BslFI GGGAC 2 cut(s) 1491, 2088
BslI CCNNNNNNNGG 8 cut(s) 195, 440, 558, 587, 802, 1486, 1525, 2494
BsmAI GTCTC 4 cut(s) 735, 1445, 1927, 2511
BsmFI GGGAC 2 cut(s) 1491, 2088
BsmI GAATGC 2 cut(s) 89, 1031
BsnI GGCC 1 cut(s) 756
Bsp119I TTCGAA 1 cut(s) 660
Bsp1286I GDGCHC 4 cut(s) 879, 1886, 2382, 2405
Bsp1407I TGTACA 1 cut(s) 1851
Bsp143I GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
Bsp1720I GCTNAGC 1 cut(s) 621
Bsp19I CCATGG 1 cut(s) 189
BspACI CCGC 4 cut(s) 40, 903, 972, 1642
BspANI GGCC 1 cut(s) 756
BspCNI CTCAG 6 cut(s) 634, 953, 1704, 1728, 1773, 2152
BspDI ATCGAT 1 cut(s) 1171
BspFNI CGCG 1 cut(s) 1443
BspLI GGNNCC 2 cut(s) 876, 2076
BspMAI CTGCAG 1 cut(s) 1881
BspMI ACCTGC 1 cut(s) 2329
BspPI GGATC 3 cut(s) 944, 2143, 2486
BspT104I TTCGAA 1 cut(s) 660
BsrDI GCAATG 2 cut(s) 992, 2467
BsrGI TGTACA 1 cut(s) 1851
BsrI ACTGG 5 cut(s) 445, 472, 552, 1503, 2004
BssECI CCNNGG 4 cut(s) 78, 189, 249, 1886
BssMI GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
BssT1I CCWWGG 2 cut(s) 189, 1886
Bst2UI CCWGG 2 cut(s) 250, 812
Bst4CI ACNGT 3 cut(s) 1289, 1851, 2303
Bst6I CTCTTC 1 cut(s) 2509
BstAPI GCANNNNNTGC 1 cut(s) 2237
BstAUI TGTACA 1 cut(s) 1851
BstBAI YACGTR 1 cut(s) 1064
BstBI TTCGAA 1 cut(s) 660
BstC8I GCNNGC 2 cut(s) 45, 1230
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 8 cut(s) 112, 547, 651, 1219, 1330, 1350, 1470, 2427
BstFNI CGCG 1 cut(s) 1443
BstHHI GCGC 2 cut(s) 1443, 1725
BstKTI GATC 7 cut(s) 238, 939, 1252, 1500, 2138, 2308, 2481
BstMAI GTCTC 4 cut(s) 735, 1445, 1927, 2511
BstMBI GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
BstMWI GCNNNNNNNGC 4 cut(s) 227, 912, 2237, 2246
BstNI CCWGG 2 cut(s) 250, 812
BstSCI CCNGG 2 cut(s) 248, 810
BstSFI CTRYAG 2 cut(s) 1877, 2395
BstSLI GKGCMC 1 cut(s) 1886
BstUI CGCG 1 cut(s) 1443
BstV1I GCAGC 5 cut(s) 205, 1626, 1702, 2135, 2193
BstV2I GAAGAC 1 cut(s) 2355
BstX2I RGATCY 3 cut(s) 936, 1497, 2135
BstYI RGATCY 3 cut(s) 936, 1497, 2135
Bsu15I ATCGAT 1 cut(s) 1171
BsuI GTATCC 1 cut(s) 2153
BsuRI GGCC 1 cut(s) 756
BsuTUI ATCGAT 1 cut(s) 1171
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 8 cut(s) 112, 547, 651, 1219, 1330, 1350, 1470, 2427
BtsI GCAGTG 5 cut(s) 1131, 1280, 1580, 1886, 2089
BveI ACCTGC 1 cut(s) 2329
Cac8I GCNNGC 2 cut(s) 45, 1230
CfoI GCGC 2 cut(s) 1443, 1725
Cfr13I GGNCC 1 cut(s) 2075
ClaI ATCGAT 1 cut(s) 1171
Csp6I GTAC 8 cut(s) 290, 315, 856, 1061, 1223, 1852, 2191, 2342
CviQI GTAC 8 cut(s) 290, 315, 856, 1061, 1223, 1852, 2191, 2342
DpnI GATC 7 cut(s) 237, 938, 1251, 1499, 2137, 2307, 2480
DpnII GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
DriI GACNNNNNGTC 1 cut(s) 2106
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 2509
Eam1105I GACNNNNNGTC 1 cut(s) 2106
EarI CTCTTC 1 cut(s) 2509
Ecl136II GAGCTC 1 cut(s) 2380
Eco130I CCWWGG 2 cut(s) 189, 1886
Eco24I GRGCYC 2 cut(s) 879, 2382
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 1 cut(s) 2075
Eco53kI GAGCTC 1 cut(s) 2380
Eco57I CTGAAG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 2380
EcoO109I RGGNCCY 1 cut(s) 2075
EcoRII CCWGG 2 cut(s) 248, 810
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 2 cut(s) 189, 1886
EcoT22I ATGCAT 1 cut(s) 1470
EcoT38I GRGCYC 2 cut(s) 879, 2382
ErhI CCWWGG 2 cut(s) 189, 1886
FalI AAGNNNNNCTT 6 cut(s) 138, 170, 1029, 1061, 1262, 1294
FaqI GGGAC 2 cut(s) 1491, 2088
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 7 cut(s) 41, 219, 904, 1640, 1691, 2149, 2207
FokI GGATG 8 cut(s) 119, 554, 658, 1226, 1337, 1357, 1477, 2434
FriOI GRGCYC 2 cut(s) 879, 2382
Fsp4HI GCNGC 7 cut(s) 41, 219, 904, 1640, 1691, 2149, 2207
FspAI RTGCGCAY 1 cut(s) 1724
FspBI CTAG 5 cut(s) 788, 839, 930, 1946, 2222
FspI TGCGCA 1 cut(s) 1724
GlaI GCGC 2 cut(s) 1442, 1724
GluI GCNGC 7 cut(s) 41, 219, 904, 1640, 1691, 2149, 2207
GsuI CTGGAG 3 cut(s) 1486, 1961, 2423
HaeIII GGCC 1 cut(s) 756
HapII CCGG 1 cut(s) 2157
HhaI GCGC 2 cut(s) 1443, 1725
Hin6I GCGC 2 cut(s) 1441, 1723
HinP1I GCGC 2 cut(s) 1441, 1723
HincII GTYRAC 2 cut(s) 781, 2452
HindII GTYRAC 2 cut(s) 781, 2452
HinfI GANTC 6 cut(s) 548, 649, 1004, 1766, 1835, 2464
HpaI GTTAAC 1 cut(s) 2452
HpaII CCGG 1 cut(s) 2157
HphI GGTGA 3 cut(s) 64, 937, 1007
Hpy166II GTNNAC 4 cut(s) 781, 1061, 1115, 2452
Hpy188I TCNGA 6 cut(s) 654, 716, 943, 1397, 1591, 2100
Hpy188III TCNNGA 5 cut(s) 703, 1799, 1832, 1940, 2440
Hpy8I GTNNAC 4 cut(s) 781, 1061, 1115, 2452
Hpy99I CGWCG 1 cut(s) 782
HpyAV CCTTC 7 cut(s) 277, 459, 1405, 1519, 1519, 2251, 2495
HpyCH4III ACNGT 3 cut(s) 1289, 1851, 2303
HpyCH4IV ACGT 3 cut(s) 854, 1063, 1865
HpyF10VI GCNNNNNNNGC 4 cut(s) 227, 912, 2237, 2246
HpySE526I ACGT 3 cut(s) 854, 1063, 1865
HspAI GCGC 2 cut(s) 1441, 1723
KspAI GTTAAC 1 cut(s) 2452
Kzo9I GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
LmnI GCTCC 8 cut(s) 273, 874, 1414, 1942, 2295, 2385, 2400, 2442
Lsp1109I GCAGC 5 cut(s) 205, 1626, 1702, 2135, 2193
LweI GCATC 4 cut(s) 217, 751, 1455, 1965
MaeI CTAG 5 cut(s) 788, 839, 930, 1946, 2222
MaeII ACGT 3 cut(s) 854, 1063, 1865
MaeIII GTNAC 6 cut(s) 70, 554, 682, 925, 1244, 2390
MalI GATC 7 cut(s) 237, 938, 1251, 1499, 2137, 2307, 2480
MboI GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
MflI RGATCY 3 cut(s) 936, 1497, 2135
MhlI GDGCHC 4 cut(s) 879, 1886, 2382, 2405
MlsI TGGCCA 1 cut(s) 756
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 2 cut(s) 1775, 1829
MmeI TCCRAC 2 cut(s) 144, 1053
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 1 cut(s) 1470
MroXI GAANNNNTTC 1 cut(s) 448
MscI TGGCCA 1 cut(s) 756
MseI TTAA 5 cut(s) 134, 1358, 2115, 2310, 2451
MslI CAYNNNNRTG 1 cut(s) 428
Msp20I TGGCCA 1 cut(s) 756
MspI CCGG 1 cut(s) 2157
MspR9I CCNGG 2 cut(s) 250, 812
Mva1269I GAATGC 2 cut(s) 89, 1031
MvaI CCWGG 2 cut(s) 250, 812
MvnI CGCG 1 cut(s) 1443
MwoI GCNNNNNNNGC 4 cut(s) 227, 912, 2237, 2246
NcoI CCATGG 1 cut(s) 189
NdeII GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
NlaIV GGNNCC 2 cut(s) 876, 2076
NmuCI GTSAC 5 cut(s) 70, 554, 682, 925, 2390
NsbI TGCGCA 1 cut(s) 1724
NsiI ATGCAT 1 cut(s) 1470
NspV TTCGAA 1 cut(s) 660
PctI GAATGC 2 cut(s) 89, 1031
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 4 cut(s) 548, 649, 1004, 2464
PflMI CCANNNNNTGG 2 cut(s) 195, 1486
PkrI GCNGC 7 cut(s) 42, 220, 905, 1641, 1692, 2150, 2208
PleI GAGTC 2 cut(s) 1774, 1829
PpsI GAGTC 2 cut(s) 1774, 1829
Ppu21I YACGTR 1 cut(s) 1064
PpuMI RGGWCCY 1 cut(s) 2075
PshBI ATTAAT 2 cut(s) 1358, 2115
Psp124BI GAGCTC 1 cut(s) 2382
Psp5II RGGWCCY 1 cut(s) 2075
Psp6I CCWGG 2 cut(s) 248, 810
PspGI CCWGG 2 cut(s) 248, 810
PspN4I GGNNCC 2 cut(s) 876, 2076
PspPI GGNCC 1 cut(s) 2075
PspPPI RGGWCCY 1 cut(s) 2075
PstI CTGCAG 1 cut(s) 1881
PsuI RGATCY 3 cut(s) 936, 1497, 2135
RsaI GTAC 8 cut(s) 291, 316, 857, 1062, 1224, 1853, 2192, 2343
RsaNI GTAC 8 cut(s) 290, 315, 856, 1061, 1223, 1852, 2191, 2342
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 1 cut(s) 2382
SalI GTCGAC 1 cut(s) 779
SaqAI TTAA 5 cut(s) 134, 1358, 2115, 2310, 2451
SatI GCNGC 7 cut(s) 41, 219, 904, 1640, 1691, 2149, 2207
Sau3AI GATC 7 cut(s) 235, 936, 1249, 1497, 2135, 2305, 2478
Sau96I GGNCC 1 cut(s) 2075
ScaI AGTACT 2 cut(s) 316, 1224
SchI GAGTC 2 cut(s) 1775, 1829
ScrFI CCNGG 2 cut(s) 250, 812
SduI GDGCHC 4 cut(s) 879, 1886, 2382, 2405
SfaNI GCATC 4 cut(s) 217, 751, 1455, 1965
SfcI CTRYAG 2 cut(s) 1877, 2395
SfuI TTCGAA 1 cut(s) 660
SinI GGWCC 1 cut(s) 2075
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 2 cut(s) 956, 2365
SmoI CTYRAG 2 cut(s) 956, 2365
SpeI ACTAGT 1 cut(s) 929
SsiI CCGC 4 cut(s) 40, 903, 972, 1642
SspI AATATT 1 cut(s) 1701
SspMI CTAG 5 cut(s) 788, 839, 930, 1946, 2222
SstI GAGCTC 1 cut(s) 2382
StyD4I CCNGG 2 cut(s) 248, 810
StyI CCWWGG 2 cut(s) 189, 1886
TaaI ACNGT 3 cut(s) 1289, 1851, 2303
TaiI ACGT 3 cut(s) 857, 1066, 1868
TaqI TCGA 5 cut(s) 211, 660, 780, 1171, 1973
TatI WGTACW 3 cut(s) 314, 1222, 1851
TauI GCSGC 2 cut(s) 43, 906
TfiI GAWTC 4 cut(s) 548, 649, 1004, 2464
Tru1I TTAA 5 cut(s) 134, 1358, 2115, 2310, 2451
Tru9I TTAA 5 cut(s) 134, 1358, 2115, 2310, 2451
TseFI GTSAC 5 cut(s) 70, 554, 682, 925, 2390
TseI GCWGC 5 cut(s) 218, 1639, 1690, 2148, 2206
Tsp45I GTSAC 5 cut(s) 70, 554, 682, 925, 2390
TspGWI ACGGA 3 cut(s) 554, 701, 766
Van91I CCANNNNNTGG 2 cut(s) 195, 1486
VpaK11BI GGWCC 1 cut(s) 2075
VspI ATTAAT 2 cut(s) 1358, 2115
XapI RAATTY 6 cut(s) 141, 945, 1552, 2024, 2408, 2443
XcmI CCANNNNNNNNNTGG 1 cut(s) 589
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 5 cut(s) 788, 839, 930, 1946, 2222
ZrmI AGTACT 2 cut(s) 316, 1224
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.