RchiOBHm_Chr2g0136341

NB-ARC domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0005191
Physical Location & Seq
Forward (+)
53700716 .. 53704902
4187 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50714

Sequence Viewer

Length: 3525 bp
ATGACCGAAATAGCAACTCACGCTGTGGGGATTGCCATCACTAAATTGTCTCATCTTGTCACCACGGAGTTTCTTGCATTCACTAATGTCAAGGATGACCTGGAATCCCTCAGAAGCACTCTAACAGATGTTCGGGAGTTGCTTGGAATTCTGGAAAGAAATCGGCCGAATTCTTCAGGTTTCGAACAGATGAACAATTGGCTCAAGAAGCTTCAAGTAGCAGCTTATGATGCAGGGGATTTAATCGCGTCCTGGGCAGTAGAATATCACCAATGGAAGACGAAGAAGCAGGTACGAAAATTCCCTCTTCCATTTAGTGCTTCAAAATTTTTCTTTCAGCATCAAGAATCATGTGACTTGAGAGAAATTACAGCGAGAATAAATAAGATTTTACAAGATGGACAAGTCTATAGAACAATCATTGGAGTGCCTAGGAGTGAAAGTTTTTCACGGCAAACTGGCTCTCTTTCTAGCAATATAATTGAGGGCAGGGGAGATGATGTAGAGAACATAATAAAACTGCTGATACCAGATGAAGAAGCCAATGATGAGGGCAGTATTGCTTTTATTCCAATTGTGGGGATGGGGGGCTTGGGTAAAAGTAAAACAACTCTCGCTCAACTCGTATATAAGAATGGCAGGGTTCATGAACATTTTAAAAGAAAATTCTGGGTTTGTGTCACAGAAACTTATGACGAGACTAGAATTCTCAAACGAATGTTGGAGTCTAATGAGATTACTTTTGATGCCAATATCACATTCGAGGTACTGCAAGACCGAGTTCGTGAACTCGTGGCTGAAAAACGATTTTTACTTGTTCTAGATGATTTATGGAACAATAATTATATGGATTTGGAGCCACTAGAAAATTTACTGAAACAAGGGGTCAGTGGAAGTAAGGTCTTGGTTACTAGTCGGAACAATGAAGTTCGGAAGATTACGGGTGCAAAATCCTCCTATAGCTTGCGACATTTCAATGATAAAGAATCACTGTCGTTATTCGAAAAAATTGCCTTTGAAGGAAGGAGTCCGCCAGATGAGTTGAAGGAATATGCAAAGCAAATTGTAAACAAGTGCAACGGTTTACCTCTGGCAGTAAAACAAATGGGGGGTCTGCTACGTGGAATCACTGATGCAAGTGAGTGGAAATATGTCGCTAGAAGTGAAATATGGGAACTAGAAAAAGATAAAGTACTGCCTGCTCTTCGATTGAGTTATAATCATCTGTCTTCAGCTCTGAAGCAATGTTTTGCGTATTGTTCCCTGTTTCCAAAAGCTTATGTTTTTGAAAAAAATGAGTTGATCAAATTATGGATGGCAGAAGCATTCATTCAACCTCATGACAGACACAGGACAGAAGACATAGGAAGCAGATACTTCAAGGAGCTGTCAGACAGGTTCTTCTTTGAGATCTCGGCGGAAGACAAGAGCAAGTGTAGAATGCATGATCTTATCCACGACTTGGCGCTATTAATTTCCTCTCCCTTTTTCTGCCAAGTGGAAGATACTGTGCCAAAGAGTTTAGACGAAGTATCTCGCCATGTATCACTGCTTTCGAATGAGGTTGAGCAGCCCCTTTCAGAAATAATAAAAAAATCAAAGAAGCTGCGCACACTTTTATTGCCAGTGAAACACTTCAAAGCCTTTGGGAAAGCTGAACGTGCAATATTCCATTCACTTAAGTACATGCGCACGCTAGATTTGAGTTCAAGCACACTTCAAGAACTGTCAGGCTCAATCGAGAATTTGAAGCTTTTGCACTACCTTGATTTATCAAAAACTGAAATAAAAAAGCTTCCCGATTCGATATGCAACCTCTGCCATTTGGAGACGTTGAAACTTTCAGATTGCCCTTGGCTTTTCACTTTGCCCAGGAAGCTCAAAACTCTTGTCAACCTGAGACATCTGGAGCTAGATGAAATGTTCTGGTACAAGGTATCAACGCTTCCAAAAAGCATGGGGTGTCTAACCAGTCTACACAATCTTCACAAATTTCAGGTTGGTCTTCTTCTCTCTTTTTCTTTGTTCCTGTTGTTCTTCTCTACACTTCATCTCCCATGTAACAAAATATACACATTTCAGGTTGGCTGCAATACAGGATACAAACTTCAAGAATTGAAGAACATGGAGTACCTTACAGGGTTCCTGCACATCTCAAACCTGGAAAATGCAGTGGATGCAGGGGAGGCCAACTTGAAAGAAAAAGAAATGATTGATAAAGTGGTATATGAATGGAGCAGCAGCAATCTGAATTTGCAAGATGAAGACGCCAAGCAGGTACTCGAAGACCTGCAGCCTCACCCGAGGGTTCAAGAGATCCAAATCTGTCACTACAGAAGCTGTGAGTTTCCAATTTGGATCAGATACGGAAAGTATGAGAACTTAACTTCTATATATTTGAATCATTGCACAAGGATCAAAATCCTCTCACTTGGCGAGTTACCCAACCTCAGAGAACTCCGTCTCAAGAACATGGCAGAAATGATGGAATGGAAGGAAGAGAACGACGTGTTCCTCTTGAGTCTAAAGATTAGTGGCTGCCCAAAGCTGACAAAACTGCCCGGCTACTTTCCTGTATTACATAGTATGAAGATCAAGAATTGTGAATCATTGGATACCCTTCCAGTTGGCCCAGTGGAATTCATAAGACTTGCTGGCAATCCTGTGCTGAAGCACTGGACTGAAGTCAATGTACTAACAAGGAGAGTTGTCGATGGTCGCCTGGTCACAAGTCATGGCATGACAAGTCATTATCTGTTGGAAGCAAACATTATCAACTGCCCTGAACTGCAGAGGTTGCCAAGAGAACTCCATCCACAGAAACTGGAAATAAGCGGCTGCAAGAACTTGCGCACCCTACCAGATGCAGACCATGCAGAATGGCTTGGGGTTTTAGCATTGGATGCATGCCATGATGAAACATTAGTTGGTATGATCCCCAGTTCTGGGAGTTTATATTCCTTGGTGATTTCTAACATCCCAAATCTCATTTGTCTTCCAAAGTGGCCCAGTCTCCCCAGCCTCCAAGCATTGTATATCCGTGATTGCACAGACCTTGAATATCTATTCAACCAAGAGAATGGGAACGGGTTGTTCGATGGCTACAACTCTCTAAAGCACCTCTCCATCCGTAACTGTCCTAAACTTCTGACACTGCCTGCTGAAGGTCTCCCAGCCAGTCTTCAAGTTTTGAGTATTGGCCAATGTGAAAGTCTCAGTTCGTTTGGCCCCAGGGAAGTACTCGCAAAACTCACATCCCTTGGTGATCTCTACCTTGAAGATTGCCCTGCACTCCAGTCCTTGCCAGAGGAAGGCCTGTCAGCGTCCCTCCAACATCTGTCCATCCAAGGATGCCCCTCGCTGGTAAGAAGCTGTGCAAAAGATGGCAGCGATTGGCCAAAGATCAAGGACATCCCCGATCTGGAAATAGAGATGCCATCTACTGAAACCACTTCAGCACCATGGTACAATCCTTTTAGGAACCGCGGAGGAAACAAGTCCAAAGGAAAGAAGGCTTCGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1174

Amino Acids

133.81

Weight (kDa)

6.67

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 8 - 98 7.5e-13 Rx N-terminal domain
NB-ARC PF00931 167 - 342 1.1e-28 NB-ARC domain
WHD_DRP PF23559 422 - 489 1.2e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 550 - 665 1.5e-13 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 703 - 825 5e-28 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1218
Acc16I TGCGCA 3 cut(s) 1610, 1691, 2852
Acc36I ACCTGC 3 cut(s) 280, 2266, 2298
AccB7I CCANNNNNTGG 1 cut(s) 1462
AccI GTMKAC 1 cut(s) 1975
AccII CGCG 2 cut(s) 248, 3486
AciI CCGC 5 cut(s) 1031, 1418, 2835, 3484, 3486
AclWI GGATC 4 cut(s) 2311, 2366, 2423, 2929
AcoI YGGCCR 3 cut(s) 164, 3199, 3395
AcuI CTGAAG 7 cut(s) 159, 1215, 1259, 2690, 2703, 3183, 3438
AcyI GRCGYC 1 cut(s) 2268
AdeI CACNNNGTG 1 cut(s) 25
AfiI CCNNNNNNNGG 8 cut(s) 578, 1462, 2945, 2946, 3164, 3311, 3361, 3421
AflII CTTAAG 1 cut(s) 1679
AflIII ACRYGT 1 cut(s) 2508
AhlI ACTAGT 1 cut(s) 911
AjiI CACGTC 1 cut(s) 2509
AjnI CCWGG 6 cut(s) 99, 251, 1871, 2160, 2721, 3230
AjuI GAANNNNNNNTTGG 4 cut(s) 743, 775, 2910, 2942
Alw26I GTCTC 8 cut(s) 54, 692, 1823, 1894, 2468, 3017, 3173, 3218
AlwI GGATC 4 cut(s) 2311, 2366, 2423, 2929
AlwNI CAGNNNCTG 2 cut(s) 2340, 2824
Ama87I CYCGRG 1 cut(s) 2302
AoxI GGCC 8 cut(s) 164, 2187, 2629, 3005, 3199, 3226, 3313, 3395
ArsI GACNNNNNNTTYG 4 cut(s) 1096, 1128, 1875, 1907
AseI ATTAAT 1 cut(s) 1472
Asp700I GAANNNNTTC 1 cut(s) 1634
AspA2I CCTAGG 1 cut(s) 431
AspLEI GCGC 4 cut(s) 1468, 1611, 1692, 2853
AspS9I GGNCC 3 cut(s) 2630, 3006, 3227
AsuC2I CCSGG 1 cut(s) 2562
AsuHPI GGTGA 5 cut(s) 52, 260, 2291, 2977, 3275
AsuII TTCGAA 3 cut(s) 183, 1002, 1556
AvaI CYCGRG 1 cut(s) 2302
AvrII CCTAGG 1 cut(s) 431
BalI TGGCCA 2 cut(s) 3201, 3397
BarI GAAGNNNNNNTAC 4 cut(s) 1929, 1961, 2676, 2708
BauI CACGAG 1 cut(s) 791
BbsI GAAGAC 9 cut(s) 284, 1221, 1365, 1428, 1997, 2271, 2292, 2987, 3173
BceAI ACGGC 1 cut(s) 467
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 6 cut(s) 101, 253, 1873, 2162, 2723, 3232
BciVI GTATCC 2 cut(s) 2093, 2608
BclI TGATCA 1 cut(s) 1302
BcnI CCSGG 1 cut(s) 2562
BcoDI GTCTC 8 cut(s) 54, 692, 1823, 1894, 2468, 3017, 3173, 3218
BcuI ACTAGT 1 cut(s) 911
BfmI CTRYAG 5 cut(s) 409, 958, 2291, 2332, 2789
BfoI RGCGCY 1 cut(s) 1469
BfrI CTTAAG 1 cut(s) 1679
BfuAI ACCTGC 3 cut(s) 280, 2266, 2298
BfuI GTATCC 2 cut(s) 2093, 2608
BglII AGATCT 1 cut(s) 1410
BlnI CCTAGG 1 cut(s) 431
BmcAI AGTACT 2 cut(s) 1194, 3240
Bme1390I CCNGG 7 cut(s) 101, 253, 1873, 2162, 2562, 2723, 3232
BmeT110I CYCGRG 1 cut(s) 2302
BmgBI CACGTC 1 cut(s) 2509
BmgT120I GGNCC 3 cut(s) 2630, 3006, 3227
BmiI GGNNCC 4 cut(s) 858, 2144, 3229, 3482
BmrFI CCNGG 7 cut(s) 101, 253, 1873, 2162, 2562, 2723, 3232
BmrI ACTGGG 3 cut(s) 2627, 2934, 3003
BmsI GCATC 9 cut(s) 220, 349, 736, 1123, 2167, 2854, 2893, 3341, 3423
BmuI ACTGGG 3 cut(s) 2627, 2934, 3003
BoxI GACNNNNGTC 1 cut(s) 2684
BpiI GAAGAC 9 cut(s) 284, 1221, 1365, 1428, 1997, 2271, 2292, 2987, 3173
BpmI CTGGAG 2 cut(s) 1928, 3278
Bpu14I TTCGAA 3 cut(s) 183, 1002, 1556
BpuEI CTTGAG 4 cut(s) 188, 379, 2450, 2539
BpuMI CCSGG 1 cut(s) 2562
BsaAI YACGTR 1 cut(s) 1121
BsaBI GATNNNNATC 3 cut(s) 35, 2321, 2420
BsaHI GRCGYC 1 cut(s) 2268
BsaI GGTCTC 1 cut(s) 3173
BsaXI ACNNNNNCTCC 6 cut(s) 427, 457, 486, 516, 2037, 2067
Bsc4I CCNNNNNNNGG 8 cut(s) 578, 1462, 2945, 2946, 3164, 3311, 3361, 3421
Bse3DI GCAATG 2 cut(s) 1250, 2404
Bse8I GATNNNNATC 3 cut(s) 35, 2321, 2420
BseBI CCWGG 6 cut(s) 101, 253, 1873, 2162, 2723, 3232
BseJI GATNNNNATC 3 cut(s) 35, 2321, 2420
BseLI CCNNNNNNNGG 8 cut(s) 578, 1462, 2945, 2946, 3164, 3311, 3361, 3421
BseMI GCAATG 2 cut(s) 1250, 2404
BseMII CTCAG 4 cut(s) 124, 1889, 2464, 3229
BseX3I CGGCCG 1 cut(s) 164
BseYI CCCAGC 2 cut(s) 3017, 3172
BsgI GTGCAG 2 cut(s) 2132, 3273
Bsh1236I CGCG 2 cut(s) 248, 3486
Bsh1285I CGRYCG 1 cut(s) 167
BshFI GGCC 8 cut(s) 166, 2189, 2631, 3007, 3201, 3228, 3315, 3397
BsiEI CGRYCG 1 cut(s) 167
BsiHKCI CYCGRG 1 cut(s) 2302
BsiSI CCGG 1 cut(s) 2562
BslFI GGGAC 1 cut(s) 3310
BslI CCNNNNNNNGG 8 cut(s) 578, 1462, 2945, 2946, 3164, 3311, 3361, 3421
BsmAI GTCTC 8 cut(s) 54, 692, 1823, 1894, 2468, 3017, 3173, 3218
BsmBI CGTCTC 2 cut(s) 1823, 2468
BsmFI GGGAC 1 cut(s) 3310
BsmI GAATGC 3 cut(s) 77, 1325, 1446
BsnI GGCC 8 cut(s) 166, 2189, 2631, 3007, 3201, 3228, 3315, 3397
Bso31I GGTCTC 1 cut(s) 3173
BsoBI CYCGRG 1 cut(s) 2302
Bsp119I TTCGAA 3 cut(s) 183, 1002, 1556
Bsp19I CCATGG 1 cut(s) 3461
BspACI CCGC 5 cut(s) 1031, 1418, 2835, 3484, 3486
BspANI GGCC 8 cut(s) 166, 2189, 2631, 3007, 3201, 3228, 3315, 3397
BspCNI CTCAG 4 cut(s) 123, 1890, 2463, 3228
BspFNI CGCG 2 cut(s) 248, 3486
BspHI TCATGA 2 cut(s) 646, 1339
BspLI GGNNCC 4 cut(s) 858, 2144, 3229, 3482
BspMAI CTGCAG 2 cut(s) 2295, 2793
BspMI ACCTGC 3 cut(s) 280, 2266, 2298
BspPI GGATC 4 cut(s) 2311, 2366, 2423, 2929
BspQI GCTCTTC 1 cut(s) 1209
BspT104I TTCGAA 3 cut(s) 183, 1002, 1556
BspTI CTTAAG 1 cut(s) 1679
BspTNI GGTCTC 1 cut(s) 3173
BsrDI GCAATG 2 cut(s) 1250, 2404
BssNI GRCGYC 1 cut(s) 2268
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 6 cut(s) 431, 1853, 2961, 3259, 3346, 3461
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 6 cut(s) 101, 253, 1873, 2162, 2723, 3232
Bst4CI ACNGT 5 cut(s) 993, 1082, 1510, 1728, 3137
Bst6I CTCTTC 3 cut(s) 312, 1209, 2493
BstACI GRCGYC 1 cut(s) 2268
BstAFI CTTAAG 1 cut(s) 1679
BstAPI GCANNNNNTGC 5 cut(s) 1818, 2177, 2797, 2873, 2903
BstBAI YACGTR 1 cut(s) 1121
BstBI TTCGAA 3 cut(s) 183, 1002, 1556
BstC8I GCNNGC 6 cut(s) 965, 1200, 1694, 2656, 2908, 3159
BstDEI CTNAG 4 cut(s) 110, 1898, 2450, 3215
BstDSI CCRYGG 3 cut(s) 63, 3461, 3484
BstENI CCTNNNNNAGG 1 cut(s) 3162
BstFNI CGCG 2 cut(s) 248, 3486
BstH2I RGCGCY 1 cut(s) 1469
BstHHI GCGC 4 cut(s) 1468, 1611, 1692, 2853
BstMAI GTCTC 8 cut(s) 54, 692, 1823, 1894, 2468, 3017, 3173, 3218
BstMCI CGRYCG 1 cut(s) 167
BstNI CCWGG 6 cut(s) 101, 253, 1873, 2162, 2723, 3232
BstNSI RCATGY 2 cut(s) 1690, 2910
BstPAI GACNNNNGTC 1 cut(s) 2684
BstSCI CCNGG 7 cut(s) 99, 251, 1871, 2160, 2560, 2721, 3230
BstSFI CTRYAG 5 cut(s) 409, 958, 2291, 2332, 2789
BstUI CGCG 2 cut(s) 248, 3486
BstV2I GAAGAC 9 cut(s) 284, 1221, 1365, 1428, 1997, 2271, 2292, 2987, 3173
BstX2I RGATCY 2 cut(s) 1410, 2316
BstXI CCANNNNNNTGG 1 cut(s) 3080
BstYI RGATCY 2 cut(s) 1410, 2316
BstZI CGGCCG 1 cut(s) 164
BsuI GTATCC 2 cut(s) 2093, 2608
BsuRI GGCC 8 cut(s) 166, 2189, 2631, 3007, 3201, 3228, 3315, 3397
BtgI CCRYGG 3 cut(s) 63, 3461, 3484
BtrI CACGTC 1 cut(s) 2509
BtsI GCAGTG 3 cut(s) 1547, 2178, 3152
BtsIMutI CAGTG 9 cut(s) 895, 989, 1128, 1547, 1632, 2178, 2640, 2674, 3152
BveI ACCTGC 3 cut(s) 280, 2266, 2298
Cac8I GCNNGC 6 cut(s) 965, 1200, 1694, 2656, 2908, 3159
CaiI CAGNNNCTG 2 cut(s) 2340, 2824
CciI TCATGA 2 cut(s) 646, 1339
CfoI GCGC 4 cut(s) 1468, 1611, 1692, 2853
Cfr13I GGNCC 3 cut(s) 2630, 3006, 3227
Cfr42I CCGCGG 1 cut(s) 3487
CseI GACGC 3 cut(s) 237, 2276, 3312
DdeI CTNAG 4 cut(s) 110, 1898, 2450, 3215
DraI TTTAAA 1 cut(s) 658
DraIII CACNNNGTG 1 cut(s) 25
EaeI YGGCCR 3 cut(s) 164, 3199, 3395
EagI CGGCCG 1 cut(s) 164
Eam1104I CTCTTC 3 cut(s) 312, 1209, 2493
EarI CTCTTC 3 cut(s) 312, 1209, 2493
EciI GGCGGA 2 cut(s) 1020, 1433
EclXI CGGCCG 1 cut(s) 164
Eco130I CCWWGG 6 cut(s) 431, 1853, 2961, 3259, 3346, 3461
Eco147I AGGCCT 1 cut(s) 3315
Eco31I GGTCTC 1 cut(s) 3173
Eco52I CGGCCG 1 cut(s) 164
Eco57I CTGAAG 7 cut(s) 159, 1215, 1259, 2690, 2703, 3183, 3438
Eco88I CYCGRG 1 cut(s) 2302
EcoNI CCTNNNNNAGG 1 cut(s) 3162
EcoRI GAATTC 4 cut(s) 147, 169, 705, 2639
EcoRII CCWGG 6 cut(s) 99, 251, 1871, 2160, 2721, 3230
EcoT14I CCWWGG 6 cut(s) 431, 1853, 2961, 3259, 3346, 3461
EcoT22I ATGCAT 2 cut(s) 1446, 2908
ErhI CCWWGG 6 cut(s) 431, 1853, 2961, 3259, 3346, 3461
Esp3I CGTCTC 2 cut(s) 1823, 2468
FaqI GGGAC 1 cut(s) 3310
FbaI TGATCA 1 cut(s) 1302
FblI GTMKAC 1 cut(s) 1975
FspAI RTGCGCAY 1 cut(s) 1691
FspI TGCGCA 3 cut(s) 1610, 1691, 2852
GlaI GCGC 4 cut(s) 1467, 1610, 1691, 2852
GsaI CCCAGC 2 cut(s) 3021, 3176
GsuI CTGGAG 2 cut(s) 1928, 3278
HaeII RGCGCY 1 cut(s) 1469
HaeIII GGCC 8 cut(s) 166, 2189, 2631, 3007, 3201, 3228, 3315, 3397
HapII CCGG 1 cut(s) 2562
HgaI GACGC 3 cut(s) 237, 2276, 3312
HhaI GCGC 4 cut(s) 1468, 1611, 1692, 2853
Hin1I GRCGYC 1 cut(s) 2268
Hin6I GCGC 4 cut(s) 1466, 1609, 1690, 2851
HinP1I GCGC 4 cut(s) 1466, 1609, 1690, 2851
HincII GTYRAC 1 cut(s) 1894
HindII GTYRAC 1 cut(s) 1894
HindIII AAGCTT 4 cut(s) 209, 1275, 1751, 1793
HpaII CCGG 1 cut(s) 2562
HphI GGTGA 5 cut(s) 52, 260, 2291, 2977, 3275
Hpy166II GTNNAC 5 cut(s) 788, 1069, 1085, 1894, 1976
Hpy8I GTNNAC 5 cut(s) 788, 1069, 1085, 1894, 1976
Hpy99I CGWCG 1 cut(s) 2510
HpyAV CCTTC 8 cut(s) 1013, 1017, 1039, 2488, 2630, 3158, 3305, 3505
HpyCH4III ACNGT 5 cut(s) 993, 1082, 1510, 1728, 3137
HpyCH4IV ACGT 4 cut(s) 1120, 1660, 1832, 2508
HpyF3I CTNAG 4 cut(s) 110, 1898, 2450, 3215
HpySE526I ACGT 4 cut(s) 1120, 1660, 1832, 2508
Hsp92I GRCGYC 1 cut(s) 2268
HspAI GCGC 4 cut(s) 1466, 1609, 1690, 2851
Ksp22I TGATCA 1 cut(s) 1302
KspI CCGCGG 1 cut(s) 3487
LguI GCTCTTC 1 cut(s) 1209
LmnI GCTCC 4 cut(s) 856, 1384, 1909, 2235
LweI GCATC 9 cut(s) 220, 349, 736, 1123, 2167, 2854, 2893, 3341, 3423
MaeII ACGT 4 cut(s) 1120, 1660, 1832, 2508
MaeIII GTNAC 9 cut(s) 58, 353, 679, 907, 2060, 2327, 2439, 2725, 3131
MfeI CAATTG 2 cut(s) 196, 573
MflI RGATCY 2 cut(s) 1410, 2316
MlsI TGGCCA 2 cut(s) 3201, 3397
MluNI TGGCCA 2 cut(s) 3201, 3397
MlyI GAGTC 3 cut(s) 734, 1036, 2530
MmeI TCCRAC 4 cut(s) 702, 896, 2739, 3355
Mox20I TGGCCA 2 cut(s) 3201, 3397
Mph1103I ATGCAT 2 cut(s) 1446, 2908
MroXI GAANNNNTTC 1 cut(s) 1634
MscI TGGCCA 2 cut(s) 3201, 3397
MseI TTAA 5 cut(s) 242, 657, 1472, 1680, 2384
MslI CAYNNNNRTG 2 cut(s) 425, 975
Msp20I TGGCCA 2 cut(s) 3201, 3397
MspA1I CMGCKG 1 cut(s) 3486
MspCI CTTAAG 1 cut(s) 1679
MspI CCGG 1 cut(s) 2562
MspR9I CCNGG 7 cut(s) 101, 253, 1873, 2162, 2562, 2723, 3232
MunI CAATTG 2 cut(s) 196, 573
Mva1269I GAATGC 3 cut(s) 77, 1325, 1446
MvaI CCWGG 6 cut(s) 101, 253, 1873, 2162, 2723, 3232
MvnI CGCG 2 cut(s) 248, 3486
NciI CCSGG 1 cut(s) 2562
NcoI CCATGG 1 cut(s) 3461
NlaIV GGNNCC 4 cut(s) 858, 2144, 3229, 3482
NmeAIII GCCGAG 1 cut(s) 1394
NmuCI GTSAC 5 cut(s) 58, 353, 679, 2327, 2725
NsbI TGCGCA 3 cut(s) 1610, 1691, 2852
NsiI ATGCAT 2 cut(s) 1446, 2908
NspI RCATGY 2 cut(s) 1690, 2910
NspV TTCGAA 3 cut(s) 183, 1002, 1556
PaeI GCATGC 1 cut(s) 2910
PagI TCATGA 2 cut(s) 646, 1339
PasI CCCWGGG 1 cut(s) 3231
PceI AGGCCT 1 cut(s) 3315
PciSI GCTCTTC 1 cut(s) 1209
PcsI WCGNNNNNNNCGW 2 cut(s) 621, 3093
PctI GAATGC 3 cut(s) 77, 1325, 1446
PdmI GAANNNNTTC 1 cut(s) 1634
PfeI GAWTC 7 cut(s) 104, 347, 986, 1125, 1802, 2401, 2606
PflMI CCANNNNNTGG 1 cut(s) 1462
PleI GAGTC 3 cut(s) 733, 1035, 2529
PpsI GAGTC 3 cut(s) 733, 1035, 2529
Ppu21I YACGTR 1 cut(s) 1121
PshAI GACNNNNGTC 1 cut(s) 2684
PshBI ATTAAT 1 cut(s) 1472
PsiI TTATAA 1 cut(s) 1218
Psp6I CCWGG 6 cut(s) 99, 251, 1871, 2160, 2721, 3230
PspFI CCCAGC 2 cut(s) 3017, 3172
PspGI CCWGG 6 cut(s) 99, 251, 1871, 2160, 2721, 3230
PspN4I GGNNCC 4 cut(s) 858, 2144, 3229, 3482
PspPI GGNCC 3 cut(s) 2630, 3006, 3227
PsrI GAACNNNNNNTAC 2 cut(s) 2114, 2146
PstI CTGCAG 2 cut(s) 2295, 2793
PstNI CAGNNNCTG 2 cut(s) 2340, 2824
PsuI RGATCY 2 cut(s) 1410, 2316
RseI CAYNNNNRTG 2 cut(s) 425, 975
SacII CCGCGG 1 cut(s) 3487
SapI GCTCTTC 1 cut(s) 1209
SaqAI TTAA 5 cut(s) 242, 657, 1472, 1680, 2384
Sau96I GGNCC 3 cut(s) 2630, 3006, 3227
ScaI AGTACT 2 cut(s) 1194, 3240
SchI GAGTC 3 cut(s) 734, 1036, 2530
ScrFI CCNGG 7 cut(s) 101, 253, 1873, 2162, 2562, 2723, 3232
SfaNI GCATC 9 cut(s) 220, 349, 736, 1123, 2167, 2854, 2893, 3341, 3423
SfcI CTRYAG 5 cut(s) 409, 958, 2291, 2332, 2789
Sfr303I CCGCGG 1 cut(s) 3487
SfuI TTCGAA 3 cut(s) 183, 1002, 1556
SgrBI CCGCGG 1 cut(s) 3487
SmiMI CAYNNNNRTG 2 cut(s) 425, 975
SmlI CTYRAG 5 cut(s) 203, 358, 1679, 2465, 2518
SmoI CTYRAG 5 cut(s) 203, 358, 1679, 2465, 2518
SpeI ACTAGT 1 cut(s) 911
SphI GCATGC 1 cut(s) 2910
SseBI AGGCCT 1 cut(s) 3315
SsiI CCGC 5 cut(s) 1031, 1418, 2835, 3484, 3486
SspI AATATT 1 cut(s) 1668
StuI AGGCCT 1 cut(s) 3315
StyD4I CCNGG 7 cut(s) 99, 251, 1871, 2160, 2560, 2721, 3230
StyI CCWWGG 6 cut(s) 431, 1853, 2961, 3259, 3346, 3461
TaaI ACNGT 5 cut(s) 993, 1082, 1510, 1728, 3137
TaiI ACGT 4 cut(s) 1123, 1663, 1835, 2511
TaqII GACCGA 2 cut(s) 20, 792
TatI WGTACW 4 cut(s) 1192, 1683, 2692, 3238
TauI GCSGC 1 cut(s) 2838
TfiI GAWTC 7 cut(s) 104, 347, 986, 1125, 1802, 2401, 2606
Tru1I TTAA 5 cut(s) 242, 657, 1472, 1680, 2384
Tru9I TTAA 5 cut(s) 242, 657, 1472, 1680, 2384
TscAI CASTG 9 cut(s) 895, 996, 1135, 1554, 1632, 2178, 2640, 2681, 3159
TseFI GTSAC 5 cut(s) 58, 353, 679, 2327, 2725
Tsp45I GTSAC 5 cut(s) 58, 353, 679, 2327, 2725
TspGWI ACGGA 5 cut(s) 80, 2382, 2450, 3029, 3119
TspRI CASTG 9 cut(s) 895, 996, 1135, 1554, 1632, 2178, 2640, 2681, 3159
Van91I CCANNNNNTGG 1 cut(s) 1462
Vha464I CTTAAG 1 cut(s) 1679
VspI ATTAAT 1 cut(s) 1472
XagI CCTNNNNNAGG 1 cut(s) 3162
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 1690, 2910
XmaJI CCTAGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 1975
XmnI GAANNNNTTC 1 cut(s) 1634
ZrmI AGTACT 2 cut(s) 1194, 3240
Zsp2I ATGCAT 2 cut(s) 1446, 2908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.