RLG00000019511

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005191
Physical Location & Seq
Forward (+)
52958385 .. 52965907
7523 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019511

Sequence Viewer

Length: 3873 bp
ATGGTTCCCATTGTTGTCTCCCTCGCAACTCCTGTTGTGACGCTTGCACTGAACAAAGTGTTATCTTTCGTCTGTGACGAGGTTTCTGCATTCTATTATGCCAAGGACGAGATAAAAAGGCTGGAGACCATTATACCAGAAATTCAAGATCAGCTTGCAAAGTTGGAAGACATTCAGCTGGATTCTCCTGGTACTGACAAGCTGAAGAATTGGGTTGAAGATCTTCAAGTTGCAGCTTATGATGCGGAGGATTTGCTCTACTCTTGGACAACAAAGTATCAACAATGGTACATGAAGAAAGAGGTAGAAAAACTCCATCTTCCATTTTGTACTTCAAAATTTCCTTTTCAGTATCTTGAATCTCGCAAGTTGAAGAAAATTATAGCGAGATTAGAAACGATTTTAAAAGATGGAAAATGTAATGAGAATCAAACAAAAGGAAGCAGAGCAACAGTTTTACAAGAAACTGGTGCTTTATTAGAATCATCCAAGCCTGTTGCTAGTAGGGAAGAAGATAAACAAGAGATAATTAGACGGCTGATAACTGATGAAGAATCTAGTAATGGGGAAAACTATTCTTTCATTCCAATTGTTGGGATGGGGGGTTTGGGTAAAACAACTCTTGCCCAACTAGTCTTCCACTATTTTCAAGACAAAGCCAAAAATGACGAGCGTAATAAACATTTCGATATTATAACCTGGGTTTCTGTGACAAAGAATTTTGATATTAGGAAGATTTTGTTTGCAATAATTTTGTCTAACAAGTCTGAAGAAGATAGAGCTCACTATGATGTCAATTACCCCTTAAGCCTACTCCAAGAAGATGGGTCTAAAACGCTGACTGGAAAAAAGTTTTTACTTGTTCTAGATGATTTATGGGCTACTGATAATCATGAGGACATGAAAAATTTAAAAGCAGTCTTAAGACTAGGGGGCAAGGGTAGTAGGGTTCTGGTGACTAGCCGAAACCAAGGAGTTTCAAAGCAGATACTGCTTTCAAAATCCCCTTATCATTTGCAATGTTTGGGTGACAATGAATCCTGGTCAATATTTGAAGAATTGGCATTTAGAAAAGATGGTATGACAGCCAGGCTGCGTGAAGAATTAGAAATACATGGAAAGGAAATTGTCAGGAAGTGCAAGGGTTTACCTCTGGCCATAAAACAAATGGCAGGTCTTTTAAGTGGGGTTACTGATGTAGCTCAGTGGAAAGATGTAGAGTTTGATCAAACATGGAAAGCAGATGATTCAGAAGAAAAGAGTAACAGTGTGCTGTCTGCTCTTAGGTTGAGTTATAATTATCTGCCTTCATCAGTTCACAAGCAGTGCTTTGCATACTGTTCCTTGTTTCCGAAAGCTCATGTATATGAGGTAGACGAATTGGTCAAATTATGGATGGCTGAAGGATTTATTGAACATAAGAAGGGGCAGAGGACAGAAGACATAGGCCGGGGTTACTTTGAAAAGCTGTCGGACAGGTTCTTCTTCGAATGTACAAGCACAGATGACGCAAGATACAGAATGCATGACCTTATCCATGACTTGGCACAATTAATATCCAGTCCCTTTTATCGCCAAGTGGTGGAAATGGAAGATATCAAGAGTTTTGATAAAAAATCTCGTCATGTATCAGTGCTTTCTGAAGAAGTTGAGCAGCCTACTTCAATGATAATCAGCAAATCCAAGAAGCTGCGGACACTTCTCTTTCCAGTTGAAGGCTTCAAAACCTTTGGGAGAGCACACACTGATATTTTCAGTTCACTTTTGTATGTGCGCGTGCTAGACCTGAGTTCAAGCACACTCGAGACACTACCAGACTCGATTGGAAAGATGAAGCTTGTGCACTACCTTGATATTTCCAAAACTGAAGTCAAAAAGCTCCCCGACTCGATATGCAACCTCTATCATTTGCAGACGTTGAAACTTTGGGGTTGCCCCTGGATATTCAGTTTGCCAAAGAAGTTCAAAATACTTGTCAACCTGAGACATCTGGAGCTAGATGACATGTTCTGGTACAAGGCATCTACATTTCCACCGAGAATGGGGTGTCTAACCAATCTGCACAATTTACACAAATTCCAGGTTGGCCGCAATACTGGGTACAAACTTGAAGAATTGAAGAACATGAAGTACCTTACAGGAAGACTTCACATCTCAAATCTGGAAAATGCTGTGAATGCAGGGAAAGAAAAGACCAATGCAGGGGAGGGGAATACGCCAGAGGTGAATGCAGAGGAGGCCAACTTGAAAGGAAAGGAAAGGATTGACAAAGTGGTATATGAATGGAGCGACAGCAAACTCAATTTGCAAAACGAAGCCATCGAAAAGAAGGTACTTGAAGACTTGGAACCTCAACCAAGGATCAAAGAGCTCCGAATCTGTCACTACAGGGGTTCTGAATTTCCAAGTTGGATGGGGGGCGGACAACTCCAGGATTTGGTTACTCTTGTTTTTACTCATTGCGAAAGAGTCAAAATCCTCTCTCTTGGCGAGCTACCCCACCTTCGAGAAGTTCATCTCGAAAGTATGCTCGAGTTGGAGGAATGGCAAGAAGAGGGGTATGAGTCATGGGACCCCGAGAAAAGTCAGAAAACCAAAGAAGAGAATTACCAACCCCTTAGAAGGCTGACGGTAAGTAATTGTCCAAAGCTGACAAAGTTGCCCCGCTGCTTTTCCAACTTAACTAATCTGAAGATCACCGAGTGTGAGAAACTGGAGAACATTCCATTTGGCCCGATGTCGTTTATCACACTTGAGGACAATGCTGTAATGAAGCACTGGGGTGAAGAAAGCCTACAAGTTTTCCTTAAGAATGTCTTGGAAGGAGTCCGAGAAACCATACCAAGAGGGCAAGAAGAAGTCATCCCGGAGGGGCGCAACGAACCCATGCCAGGAGAGAGAGAAGAAGTCATCCCAGAGGGGCACACCGAACCCATACCAGGAGAGAGAGAAGAAGTCATCCCGGAGGAGCACACCGAACCCAGACCAAGAGAGATAGAAGAAGTCATCTCGGAAGGGCACAACGAACCGATACCAAGATATATAGAAGAAGCCATCTTGGAGGGGCATAACGAACCCATACCAAGAGAGCAACAAGAAGCTATCTTGGAGGGGCACAGCATACCAATTCCAAGAAAGGTAATTATATTAGCTTTCTCATCAAAAATGATCAATCTGAAGATCGTGAAATGCCCTGCAATGAGTAAGTTGCCAGGAGGGCTCCTACCACAGAAATTGGAAATAAGCGGGTGCAGTTCACTGACTGCCCTCCCAGAAGAAAACTGCGCAGGACGCTTGACTCAATTAGCATTGGATTCATGTCAGGGCGACACATTATTGGGTACGATACCCAGTAGCCCCAAGTTACTGTCTTTAATGATTACAAATATCTCAGATATCCGCTGCCTTCCGACTTTTCCAGGACTCCCCGAACTCCAAACGCTGCACATCCATGACTTTCCAGATCTCGAGTACCTATCCACTCCAGAAAACAGGTTATTCGAACGCTTCACCTGTCTTCGTATACTTTCCTTCAAGAACTGTCCGAAGCTTATGACACTGCCAGCCGAAGGGCTCCCAACCAGTCTTCGGTATTTGAGTATCGGCCCGTGTGACTGTCTCAATTCATTTGGCCCCTCCGACGTACTCGTGGCCCAGTCTTCCCAACCTCGCGCAGACCTTGAATATCCATCCAACCAAGAGAAGAACATGTTGCTCAAGAGCTTCAACTCTCTAAAGCGTCTCTCCATCCGTAACTGTCCTGAACTTCTGACACTACCAGCTGAATGTCTCCCAACCAGTCCTCAAGTTTTGAGTATTGGCCATGTGCAAGTCTCAGTTGGTTTGGCCCCGGAGAAGTACTTTCAAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1291

Amino Acids

147.24

Weight (kDa)

5.78

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 96 1.5e-10 Rx N-terminal domain
NB-ARC PF00931 170 - 358 1.3e-32 NB-ARC domain
WHD_DRP PF23559 449 - 516 1.8e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 585 - 879 2.6e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 703 - 843 1.5e-27 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 695, 1296
AasI GACNNNNNNGTC 1 cut(s) 1382
Acc16I TGCGCA 1 cut(s) 3286
Acc36I ACCTGC 1 cut(s) 1163
AccB7I CCANNNNNTGG 4 cut(s) 593, 1543, 1582, 2437
AccI GTMKAC 2 cut(s) 1374, 3523
AccII CGCG 2 cut(s) 1776, 3672
AciI CCGC 7 cut(s) 245, 1693, 2089, 2421, 2665, 3246, 3400
AclWI GGATC 1 cut(s) 2369
AcoI YGGCCR 3 cut(s) 1155, 2086, 3820
AcsI RAATTY 6 cut(s) 141, 338, 718, 907, 2075, 2399
AcuI CTGAAG 7 cut(s) 224, 789, 1422, 1662, 1887, 2711, 3197
AdeI CACNNNGTG 1 cut(s) 2703
AflII CTTAAG 3 cut(s) 805, 922, 2807
AflIII ACRYGT 2 cut(s) 2004, 3708
AhlI ACTAGT 1 cut(s) 631
AleI CACNNNNGTG 1 cut(s) 2781
Alw21I GWGCWC 5 cut(s) 784, 1741, 1845, 2373, 2973
Alw26I GTCTC 8 cut(s) 22, 119, 1799, 1978, 3623, 3746, 3794, 3838
Alw44I GTGCAC 1 cut(s) 1841
AlwI GGATC 1 cut(s) 2369
AlwNI CAGNNNCTG 1 cut(s) 991
Ama87I CYCGRG 4 cut(s) 1802, 2531, 2576, 3467
ApaLI GTGCAC 1 cut(s) 1841
ApeKI GCWGC 7 cut(s) 233, 1093, 1654, 1690, 2667, 3402, 3442
ApoI RAATTY 6 cut(s) 141, 338, 718, 907, 2075, 2399
ArsI GACNNNNNNTTYG 6 cut(s) 1159, 1191, 1854, 1886, 1959, 1991
AseI ATTAAT 1 cut(s) 1553
Asp700I GAANNNNTTC 2 cut(s) 171, 222
AspLEI GCGC 4 cut(s) 1776, 2877, 3287, 3674
AspS9I GGNCC 6 cut(s) 2572, 2732, 3605, 3632, 3652, 3847
AsuC2I CCSGG 4 cut(s) 1451, 2867, 2963, 3851
AsuHPI GGTGA 6 cut(s) 967, 1040, 2236, 2689, 2795, 3502
AsuII TTCGAA 2 cut(s) 1488, 3501
AvaI CYCGRG 4 cut(s) 1802, 2531, 2576, 3467
AvaII GGWCC 1 cut(s) 2572
BaeGI GKGCMC 4 cut(s) 1845, 2925, 3021, 3117
BalI TGGCCA 2 cut(s) 1157, 3822
BanII GRGCYC 4 cut(s) 784, 2373, 3222, 3576
BarI GAAGNNNNNNTAC 2 cut(s) 2778, 2810
BauI CACGAG 1 cut(s) 3647
BbsI GAAGAC 8 cut(s) 174, 628, 1446, 2149, 2346, 3509, 3578, 3651
Bbv12I GWGCWC 5 cut(s) 784, 1741, 1845, 2373, 2973
BbvI GCAGC 7 cut(s) 245, 1080, 1666, 1677, 2654, 3389, 3429
BceAI ACGGC 1 cut(s) 551
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BclI TGATCA 2 cut(s) 1225, 3168
BcnI CCSGG 4 cut(s) 1451, 2867, 2963, 3851
BcoDI GTCTC 8 cut(s) 22, 119, 1799, 1978, 3623, 3746, 3794, 3838
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 8 cut(s) 501, 558, 632, 866, 929, 960, 1781, 1997
BfmI CTRYAG 1 cut(s) 2386
BfrI CTTAAG 3 cut(s) 805, 922, 2807
BfuAI ACCTGC 1 cut(s) 1163
BglI GCCNNNNNGGC 1 cut(s) 3217
BglII AGATCT 2 cut(s) 220, 3463
BisI GCNGC 8 cut(s) 234, 1094, 1655, 1691, 2089, 2668, 3403, 3443
BlsI GCNGC 8 cut(s) 235, 1095, 1656, 1692, 2090, 2669, 3404, 3444
BmcAI AGTACT 1 cut(s) 3860
Bme18I GGWCC 1 cut(s) 2572
BmeT110I CYCGRG 4 cut(s) 1802, 2531, 2576, 3467
BmgT120I GGNCC 6 cut(s) 2572, 2732, 3605, 3632, 3652, 3847
BmiI GGNNCC 8 cut(s) 6, 2349, 2573, 2574, 3221, 3575, 3634, 3849
BmrI ACTGGG 4 cut(s) 2106, 2788, 3345, 3649
BmsI GCATC 2 cut(s) 232, 2030
BmuI ACTGGG 4 cut(s) 2106, 2788, 3345, 3649
BpiI GAAGAC 8 cut(s) 174, 628, 1446, 2149, 2346, 3509, 3578, 3651
BpmI CTGGAG 5 cut(s) 143, 2012, 2414, 2735, 3468
Bpu14I TTCGAA 2 cut(s) 1488, 3501
BpuEI CTTGAG 3 cut(s) 2774, 3701, 3789
BpuMI CCSGG 4 cut(s) 1451, 2867, 2963, 3851
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 7 cut(s) 102, 699, 970, 1450, 1937, 2357, 3849
Bse3DI GCAATG 3 cut(s) 1025, 2458, 3204
BseDI CCNNGG 7 cut(s) 102, 699, 970, 1450, 1937, 2357, 3849
BseMI GCAATG 3 cut(s) 1025, 2458, 3204
BseMII CTCAG 5 cut(s) 1217, 1778, 1973, 3405, 3849
BseRI GAGGAG 2 cut(s) 2249, 2981
BseSI GKGCMC 4 cut(s) 1845, 2925, 3021, 3117
BseXI GCAGC 7 cut(s) 245, 1080, 1666, 1677, 2654, 3389, 3429
BsgI GTGCAG 3 cut(s) 2045, 3271, 3428
Bsh1236I CGCG 2 cut(s) 1776, 3672
BsiHKAI GWGCWC 5 cut(s) 784, 1741, 1845, 2373, 2973
BsiHKCI CYCGRG 4 cut(s) 1802, 2531, 2576, 3467
BsiSI CCGG 4 cut(s) 1450, 2867, 2963, 3851
BslFI GGGAC 2 cut(s) 1548, 2585
BsmAI GTCTC 8 cut(s) 22, 119, 1799, 1978, 3623, 3746, 3794, 3838
BsmBI CGTCTC 1 cut(s) 3746
BsmFI GGGAC 2 cut(s) 1548, 2585
BsmI GAATGC 4 cut(s) 89, 1527, 2182, 2233
Bso31I GGTCTC 1 cut(s) 119
BsoBI CYCGRG 4 cut(s) 1802, 2531, 2576, 3467
Bsp119I TTCGAA 2 cut(s) 1488, 3501
Bsp1407I TGTACA 1 cut(s) 1493
Bsp143I GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
BspACI CCGC 7 cut(s) 245, 1693, 2089, 2421, 2665, 3246, 3400
BspCNI CTCAG 5 cut(s) 1216, 1779, 1974, 3404, 3848
BspFNI CGCG 2 cut(s) 1776, 3672
BspHI TCATGA 1 cut(s) 892
BspLI GGNNCC 8 cut(s) 6, 2349, 2573, 2574, 3221, 3575, 3634, 3849
BspMI ACCTGC 1 cut(s) 1163
BspPI GGATC 1 cut(s) 2369
BspT104I TTCGAA 2 cut(s) 1488, 3501
BspTI CTTAAG 3 cut(s) 805, 922, 2807
BspTNI GGTCTC 1 cut(s) 119
BsrDI GCAATG 3 cut(s) 1025, 2458, 3204
BsrGI TGTACA 1 cut(s) 1493
BssECI CCNNGG 7 cut(s) 102, 699, 970, 1450, 1937, 2357, 3849
BssMI GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
BssNAI GTATAC 1 cut(s) 3524
BssSI CACGAG 1 cut(s) 3647
BssT1I CCWWGG 3 cut(s) 102, 970, 2357
Bst1107I GTATAC 1 cut(s) 3524
Bst2BI CACGAG 1 cut(s) 3647
Bst4CI ACNGT 8 cut(s) 454, 1268, 1340, 2632, 3369, 3542, 3617, 3758
Bst6I CTCTTC 2 cut(s) 2547, 2595
BstAFI CTTAAG 3 cut(s) 805, 922, 2807
BstAPI GCANNNNNTGC 1 cut(s) 991
BstAUI TGTACA 1 cut(s) 1493
BstBI TTCGAA 2 cut(s) 1488, 3501
BstC8I GCNNGC 5 cut(s) 45, 156, 1778, 2492, 3564
BstDEI CTNAG 7 cut(s) 1203, 1283, 1787, 1982, 2618, 3391, 3835
BstFNI CGCG 2 cut(s) 1776, 3672
BstHHI GCGC 4 cut(s) 1776, 2877, 3287, 3674
BstKTI GATC 8 cut(s) 151, 223, 1228, 2364, 2697, 3171, 3183, 3466
BstMAI GTCTC 8 cut(s) 22, 119, 1799, 1978, 3623, 3746, 3794, 3838
BstMBI GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
BstMWI GCNNNNNNNGC 6 cut(s) 242, 991, 2177, 2237, 3217, 3291
BstNSI RCATGY 2 cut(s) 2008, 3712
BstSFI CTRYAG 1 cut(s) 2386
BstSLI GKGCMC 4 cut(s) 1845, 2925, 3021, 3117
BstUI CGCG 2 cut(s) 1776, 3672
BstV1I GCAGC 7 cut(s) 245, 1080, 1666, 1677, 2654, 3389, 3429
BstV2I GAAGAC 8 cut(s) 174, 628, 1446, 2149, 2346, 3509, 3578, 3651
BstX2I RGATCY 2 cut(s) 220, 3463
BstXI CCANNNNNNTGG 1 cut(s) 824
BstYI RGATCY 2 cut(s) 220, 3463
BstZ17I GTATAC 1 cut(s) 3524
BtsI GCAGTG 2 cut(s) 1331, 3557
BtsIMutI CAGTG 9 cut(s) 47, 1211, 1273, 1331, 1638, 1743, 2776, 3257, 3557
BveI ACCTGC 1 cut(s) 1163
Cac8I GCNNGC 5 cut(s) 45, 156, 1778, 2492, 3564
CaiI CAGNNNCTG 1 cut(s) 991
CciI TCATGA 1 cut(s) 892
CfoI GCGC 4 cut(s) 1776, 2877, 3287, 3674
Cfr13I GGNCC 6 cut(s) 2572, 2732, 3605, 3632, 3652, 3847
CseI GACGC 4 cut(s) 49, 1517, 3300, 3728
CspCI CAANNNNNGTGG 2 cut(s) 3216, 3251
DdeI CTNAG 7 cut(s) 1203, 1283, 1787, 1982, 2618, 3391, 3835
DpnI GATC 8 cut(s) 150, 222, 1227, 2363, 2696, 3170, 3182, 3465
DpnII GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
DraI TTTAAA 2 cut(s) 405, 912
DraIII CACNNNGTG 1 cut(s) 2703
DrdI GACNNNNNNGTC 1 cut(s) 1382
DseDI GACNNNNNNGTC 1 cut(s) 1382
EaeI YGGCCR 3 cut(s) 1155, 2086, 3820
Eam1104I CTCTTC 2 cut(s) 2547, 2595
EarI CTCTTC 2 cut(s) 2547, 2595
EciI GGCGGA 1 cut(s) 2436
Ecl136II GAGCTC 2 cut(s) 782, 2371
Eco130I CCWWGG 3 cut(s) 102, 970, 2357
Eco24I GRGCYC 4 cut(s) 784, 2373, 3222, 3576
Eco31I GGTCTC 1 cut(s) 119
Eco32I GATATC 2 cut(s) 1597, 3397
Eco47I GGWCC 1 cut(s) 2572
Eco53kI GAGCTC 2 cut(s) 782, 2371
Eco57I CTGAAG 7 cut(s) 224, 789, 1422, 1662, 1887, 2711, 3197
Eco88I CYCGRG 4 cut(s) 1802, 2531, 2576, 3467
EcoICRI GAGCTC 2 cut(s) 782, 2371
EcoO109I RGGNCCY 1 cut(s) 2572
EcoRV GATATC 2 cut(s) 1597, 3397
EcoT14I CCWWGG 3 cut(s) 102, 970, 2357
EcoT22I ATGCAT 1 cut(s) 1527
EcoT38I GRGCYC 4 cut(s) 784, 2373, 3222, 3576
ErhI CCWWGG 3 cut(s) 102, 970, 2357
Esp3I CGTCTC 1 cut(s) 3746
FalI AAGNNNNNCTT 8 cut(s) 138, 170, 1313, 1345, 2790, 2822, 2801, 2833
FaqI GGGAC 2 cut(s) 1548, 2585
FauI CCCGC 2 cut(s) 2672, 3239
FbaI TGATCA 2 cut(s) 1225, 3168
FblI GTMKAC 2 cut(s) 1374, 3523
Fnu4HI GCNGC 8 cut(s) 234, 1094, 1655, 1691, 2089, 2668, 3403, 3443
FriOI GRGCYC 4 cut(s) 784, 2373, 3222, 3576
Fsp4HI GCNGC 8 cut(s) 234, 1094, 1655, 1691, 2089, 2668, 3403, 3443
FspBI CTAG 8 cut(s) 501, 558, 632, 866, 929, 960, 1781, 1997
FspI TGCGCA 1 cut(s) 3286
GlaI GCGC 4 cut(s) 1775, 2876, 3286, 3673
GluI GCNGC 8 cut(s) 234, 1094, 1655, 1691, 2089, 2668, 3403, 3443
GsuI CTGGAG 5 cut(s) 143, 2012, 2414, 2735, 3468
HapII CCGG 4 cut(s) 1450, 2867, 2963, 3851
HgaI GACGC 4 cut(s) 49, 1517, 3300, 3728
HhaI GCGC 4 cut(s) 1776, 2877, 3287, 3674
Hin6I GCGC 4 cut(s) 1774, 2875, 3285, 3672
HinP1I GCGC 4 cut(s) 1774, 2875, 3285, 3672
HincII GTYRAC 1 cut(s) 1978
HindII GTYRAC 1 cut(s) 1978
HindIII AAGCTT 2 cut(s) 1835, 3548
HpaII CCGG 4 cut(s) 1450, 2867, 2963, 3851
HphI GGTGA 6 cut(s) 967, 1040, 2236, 2689, 2795, 3502
Hpy166II GTNNAC 8 cut(s) 1148, 1318, 1375, 1760, 1843, 1978, 3257, 3524
Hpy8I GTNNAC 8 cut(s) 1148, 1318, 1375, 1760, 1843, 1978, 3257, 3524
Hpy99I CGWCG 1 cut(s) 3644
HpyCH4III ACNGT 8 cut(s) 454, 1268, 1340, 2632, 3369, 3542, 3617, 3758
HpyCH4IV ACGT 2 cut(s) 1916, 3642
HpyF10VI GCNNNNNNNGC 6 cut(s) 242, 991, 2177, 2237, 3217, 3291
HpyF3I CTNAG 7 cut(s) 1203, 1283, 1787, 1982, 2618, 3391, 3835
HpySE526I ACGT 2 cut(s) 1916, 3642
HspAI GCGC 4 cut(s) 1774, 2875, 3285, 3672
KflI GGGWCCC 1 cut(s) 2572
Ksp22I TGATCA 2 cut(s) 1225, 3168
Kzo9I GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
LmnI GCTCC 7 cut(s) 1884, 1993, 2286, 2376, 2968, 3225, 3579
Lsp1109I GCAGC 7 cut(s) 245, 1080, 1666, 1677, 2654, 3389, 3429
LweI GCATC 2 cut(s) 232, 2030
MaeI CTAG 8 cut(s) 501, 558, 632, 866, 929, 960, 1781, 1997
MaeII ACGT 2 cut(s) 1916, 3642
MalI GATC 8 cut(s) 150, 222, 1227, 2363, 2696, 3170, 3182, 3465
MboI GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 220, 3463
MlsI TGGCCA 2 cut(s) 1157, 3822
MluNI TGGCCA 2 cut(s) 1157, 3822
MlyI GAGTC 7 cut(s) 1811, 1880, 2478, 2573, 2835, 3292, 3417
MmeI TCCRAC 8 cut(s) 144, 1452, 2390, 2517, 2700, 3434, 3663, 3717
Mox20I TGGCCA 2 cut(s) 1157, 3822
Mph1103I ATGCAT 1 cut(s) 1527
MroXI GAANNNNTTC 2 cut(s) 171, 222
MscI TGGCCA 2 cut(s) 1157, 3822
MseI TTAA 9 cut(s) 404, 806, 911, 923, 1181, 1553, 2681, 2808, 3374
MslI CAYNNNNRTG 4 cut(s) 789, 1365, 2781, 3450
Msp20I TGGCCA 2 cut(s) 1157, 3822
MspA1I CMGCKG 4 cut(s) 178, 2667, 3402, 3782
MspCI CTTAAG 3 cut(s) 805, 922, 2807
MspI CCGG 4 cut(s) 1450, 2867, 2963, 3851
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 4 cut(s) 89, 1527, 2182, 2233
MvnI CGCG 2 cut(s) 1776, 3672
MwoI GCNNNNNNNGC 6 cut(s) 242, 991, 2177, 2237, 3217, 3291
NciI CCSGG 4 cut(s) 1451, 2867, 2963, 3851
NdeII GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
NlaIV GGNNCC 8 cut(s) 6, 2349, 2573, 2574, 3221, 3575, 3634, 3849
NmuCI GTSAC 7 cut(s) 37, 74, 709, 955, 1028, 2381, 3611
NsbI TGCGCA 1 cut(s) 3286
NsiI ATGCAT 1 cut(s) 1527
NspI RCATGY 2 cut(s) 2008, 3712
NspV TTCGAA 2 cut(s) 1488, 3501
OliI CACNNNNGTG 1 cut(s) 2781
PaeR7I CTCGAG 3 cut(s) 1802, 2531, 3467
PagI TCATGA 1 cut(s) 892
PciI ACATGT 2 cut(s) 2004, 3708
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 2319
PctI GAATGC 4 cut(s) 89, 1527, 2182, 2233
PdmI GAANNNNTTC 2 cut(s) 171, 222
PfeI GAWTC 9 cut(s) 182, 359, 427, 482, 554, 1037, 1247, 2376, 3314
PflMI CCANNNNNTGG 4 cut(s) 593, 1543, 1582, 2437
PfoI TCCNGGA 4 cut(s) 2430, 2865, 2961, 3418
PkrI GCNGC 8 cut(s) 235, 1095, 1656, 1692, 2090, 2669, 3404, 3444
PleI GAGTC 7 cut(s) 1811, 1880, 2477, 2572, 2834, 3292, 3417
PpsI GAGTC 7 cut(s) 1811, 1880, 2477, 2572, 2834, 3292, 3417
PpuMI RGGWCCY 1 cut(s) 2572
PscI ACATGT 2 cut(s) 2004, 3708
PshBI ATTAAT 1 cut(s) 1553
PsiI TTATAA 2 cut(s) 695, 1296
Psp124BI GAGCTC 2 cut(s) 784, 2373
Psp5II RGGWCCY 1 cut(s) 2572
PspN4I GGNNCC 8 cut(s) 6, 2349, 2573, 2574, 3221, 3575, 3634, 3849
PspPI GGNCC 6 cut(s) 2572, 2732, 3605, 3632, 3652, 3847
PspPPI RGGWCCY 1 cut(s) 2572
PspXI VCTCGAGB 1 cut(s) 2531
PsrI GAACNNNNNNTAC 2 cut(s) 2114, 2146
PstNI CAGNNNCTG 1 cut(s) 991
PsuI RGATCY 2 cut(s) 220, 3463
PvuII CAGCTG 2 cut(s) 178, 3782
RseI CAYNNNNRTG 4 cut(s) 789, 1365, 2781, 3450
SacI GAGCTC 2 cut(s) 784, 2373
SaqAI TTAA 9 cut(s) 404, 806, 911, 923, 1181, 1553, 2681, 2808, 3374
SatI GCNGC 8 cut(s) 234, 1094, 1655, 1691, 2089, 2668, 3403, 3443
Sau3AI GATC 8 cut(s) 148, 220, 1225, 2361, 2694, 3168, 3180, 3463
Sau96I GGNCC 6 cut(s) 2572, 2732, 3605, 3632, 3652, 3847
ScaI AGTACT 1 cut(s) 3860
SchI GAGTC 7 cut(s) 1811, 1880, 2478, 2573, 2835, 3292, 3417
SfaNI GCATC 2 cut(s) 232, 2030
SfcI CTRYAG 1 cut(s) 2386
Sfr274I CTCGAG 3 cut(s) 1802, 2531, 3467
SfuI TTCGAA 2 cut(s) 1488, 3501
SinI GGWCC 1 cut(s) 2572
SlaI CTCGAG 3 cut(s) 1802, 2531, 3467
SmiMI CAYNNNNRTG 4 cut(s) 789, 1365, 2781, 3450
SmlI CTYRAG 9 cut(s) 805, 922, 1802, 2531, 2753, 2807, 3467, 3716, 3804
SmoI CTYRAG 9 cut(s) 805, 922, 1802, 2531, 2753, 2807, 3467, 3716, 3804
SpeI ACTAGT 1 cut(s) 631
SsiI CCGC 7 cut(s) 245, 1693, 2089, 2421, 2665, 3246, 3400
SspI AATATT 1 cut(s) 1050
SspMI CTAG 8 cut(s) 501, 558, 632, 866, 929, 960, 1781, 1997
SstI GAGCTC 2 cut(s) 784, 2373
StyI CCWWGG 3 cut(s) 102, 970, 2357
TaaI ACNGT 8 cut(s) 454, 1268, 1340, 2632, 3369, 3542, 3617, 3758
TaiI ACGT 2 cut(s) 1919, 3645
TatI WGTACW 3 cut(s) 329, 1493, 3858
TauI GCSGC 1 cut(s) 2091
TfiI GAWTC 9 cut(s) 182, 359, 427, 482, 554, 1037, 1247, 2376, 3314
Tru1I TTAA 9 cut(s) 404, 806, 911, 923, 1181, 1553, 2681, 2808, 3374
Tru9I TTAA 9 cut(s) 404, 806, 911, 923, 1181, 1553, 2681, 2808, 3374
TscAI CASTG 9 cut(s) 54, 1211, 1273, 1331, 1638, 1750, 2783, 3264, 3564
TseFI GTSAC 7 cut(s) 37, 74, 709, 955, 1028, 2381, 3611
TseI GCWGC 7 cut(s) 233, 1093, 1654, 1690, 2667, 3402, 3442
Tsp45I GTSAC 7 cut(s) 37, 74, 709, 955, 1028, 2381, 3611
TspGWI ACGGA 1 cut(s) 3740
TspRI CASTG 9 cut(s) 54, 1211, 1273, 1331, 1638, 1750, 2783, 3264, 3564
Van91I CCANNNNNTGG 4 cut(s) 593, 1543, 1582, 2437
Vha464I CTTAAG 3 cut(s) 805, 922, 2807
VneI GTGCAC 1 cut(s) 1841
VpaK11BI GGWCC 1 cut(s) 2572
VspI ATTAAT 1 cut(s) 1553
XapI RAATTY 6 cut(s) 141, 338, 718, 907, 2075, 2399
XbaI TCTAGA 1 cut(s) 865
XceI RCATGY 2 cut(s) 2008, 3712
XcmI CCANNNNNNNNNTGG 1 cut(s) 1165
XhoI CTCGAG 3 cut(s) 1802, 2531, 3467
XmiI GTMKAC 2 cut(s) 1374, 3523
XmnI GAANNNNTTC 2 cut(s) 171, 222
XspI CTAG 8 cut(s) 501, 558, 632, 866, 929, 960, 1781, 1997
ZrmI AGTACT 1 cut(s) 3860
Zsp2I ATGCAT 1 cut(s) 1527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.