Rw2G031000

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005191
Physical Location & Seq
Forward (+)
50960702 .. 50965730
5029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G031000.1

Sequence Viewer

Length: 4032 bp
ATGGTTCCCATTGTTGTCGCCCTTGCAGCTCCTGTTGTGAAGCTTGCACTGGAAAAAGTGTTATCTTTCGTCAGCGACGAGGGTTTTGCATTCTATCATGCCAAGGACGAGATAAAAAGGCTGGGGACCATTGTACCAGAAATTCAAGATCAGCTTGCAAAGTTGGAAGACGATCGGCTGGATCCTTCTAGTACTTCCAGGCTGAAGAATTGGTTTGAAAATCTTCGAGTTGCAGCTTATGATGCGGAGGATTTGCTCTACACTTGGACAACAAAGTATCAAAAATGGAACATGAAGAAAGAGGTAGAAAAACTCCACCTTCCATTTAGTATGTCAACCACTTCTTTTCAGTATTTTGAATCTCGCAAGTTGAAGAAAATTATAGCGAGATTGGATAAGATTGTAGAAGAAGGAAAATGTTATGGGATTCAAACAATAGGAAGTAGAGCACCGGTTTCACCAGAAACTAGTGATTTTTTAGAGTCATCCGAGCCTGTTGTTAGTAGGGAAGAAGATAAACAAGAGATAATTAGACGGCTGATAACTGATGAAGAATCTAGTAATGGGGAAAACTATTCTTTCATTCCAATTGTTGGGATGGGGGGTTTGGGTAAAACAACTCTTGCCCAACTAGTATTCCACTATTTTAAAGAGAAAGCCAAAAATGACAAGCCTAATAAACATTTCGATATTAGAACCTGGGTTTCTGTGACAAAGAATTTTGATATAAAGAAGATTTTGACTGAAATTATTTTGTCCAACAAGTCTGAAGAAGACATAGTGACCACTAATGTCAATTCATCCATGCGCCTACTCCAAAACGATGCGTCTAAAACGCTGACTGGAAAAAAGTTTTTACTTGTTCTCGATGATTTATGGGATACTGATAATCATAAGGACATGAAAAATTTAAAAGCAGTCTTAAGACTAGGGGGCAAGGGTAGTAGGGTTCTGGTGACTAGTCGAAACCAAGGAGTTTCAAAGCAGATACTGCTTTCAAAATCCCCTTATCATTTGCAATGTTTGGGTGACGATGAATCCTGGTCAATGTTTAAAGAATTTGCATTTAGAAAAGATGGGATGACAGCTAGGCTGCGTGAAGATTTAGAAATACATGGAAAGGAAATTGTCAGAAAGTGCAGGGGTTTACCTTTGGCCATAAAACAAATGGCAGGTCTTTTAAGTGGGGTTACTGATGTAGCTCGGTGGAGAGCTATAGAGTCAAGTCAAACATGGGAAGTAGATGATTCAGATTACGAAAACAGTTATAATGTACTGTCTGCTCTTAGGTTGAGTTATAATTATCTGCCTTCATCAGTTCACAAGCAGTGCTTTGCATACTGTTCCTTGTTTCCAAAAGCTCATGTATATGAGGTAGACGAATTGGTCAAATTATGGATGGCTGAAGGATTTATTGAACCTGAGAAGGGACAGAGGACAGAAGAAATAGGCCGCCATTACTTTGAGAAGCTGGCAGACAGGTTCTTCTTCGAATGTACTAGCACAGATGACGCAAAGTACAGAATGCACGACCTTATTCATGACTTGGCACAATTAATTTCCAGTCCCCTTTATCGCCAAGTGGTGGAAATGGAAGATATTGAGAGTTTTGATAAAAAATCTCGTCATGTATCAGTACTTTCTGAAGAAGTTGAGCAGCCTACTTTAATGATAATCAGCAAATCCAAGAAGCTGCGGACACTTCTCTTTCCAGTTGAACGCTTCAAAACCTTTGGGAGAGCATACACTGATATTTTCAGTTCACTTGTGTACGTGCGCGTGCTAGACCTGGGTTCAAGCACACTCGAGACACTACCAGACTCGATTGGAGAGATGAAGCTTGTGCACTACCTTGATATATCCAAGACTGAAGTCAAAAAGCTCCCCGATTCGATATGCAACCTCTATCATTTGCAGACGTTGAAACTTAGGGGTTGCCCCTGGATATTCAGTTTGCCGAATAAGATCAAAATGCTTGTCAACCTGAGACATCTGGAGCTAGATGACATGTTCTGGTACAAGGCATCTACATTTCCACCGAGCATGGGGTGTCTAACCAATCTGCACAATTTACACAAATTTCAGGTTGGCCGCGATACTGGGTACAAACTCAAAGAATTGAAGAACATGAAGTATCTTACAGGAAGACTTCACATCTCAAATCTGGAAAATGCTGTGAATGCAGGAAAAGAAAAGACCAGTGCAGGGGAGGGGAATACGCCACAGGTGAATGCAGAGGAGGCCAACTTGAAAGGAAAGAAACGGATTGAGAAAGTGGAATATGAATGGAGCGACAGCACACTCAATTTGCAAAACGAAGCCATCGAAAAGAAGGTACTTGAAGACTTGGAACCTCAACCAAGGATCAAAGAGCTCCGAATCTGTCACTACAGGGGTTCTGAATTTCCAAGTTGGATGGGGGGCGGGCAACTCCAGGATTTGGTTACTCTTGTTTTGACTCATTGCGAAAGAGTCAAAATCCTCTCTCTTGGCGAGCTACCCCACCTTCAAGAAGTTCATCTCGAAAGTATGCTTGAGTTGGAGGAATGGCAAGAAGAGGGGTATGAGTCCCTAGACCTGGAGAAAACCGAAGAAGAGAATTACCAACCCCTCAGAAGGCTGACAGTGAGTAATTGCCCAAAGCTCACAAAGTTGCCCTGCTGCTTTTCCAAATTATATGTTCTGAAGATCACAAAGTGTGTGAAATTGGAGAACATTCCATTTGGCCCGATGTCGTTTATTACTCTGGAGGACAATGCTGTTATGAAGCACTGGGGTGAAGAAAGCCTACAAGTTTTCTTTAAGAATTTCTTGGAAGAAGTCATCTCGGACAGGCACAACGAAACCATACCAAGAGGGCTAGAAGAAGTCATCCCGGAGGGGCACAACGAATCCATACCAGGAGAGATAGAAGAAGTCATCCCGGAGGGGCACACCGAATCCATACCAGGAGAGGTAGAAGAAGTCATCCCGGACAGGCACAACGAATCCATACCAGGAGAGATAGAAGAAGTCATCCCGGAGGGGAACACCGAACCCATACCAGAAGAGATAGAAGAAGTGATCCCGGAGGAGCACAACGAACCCATACCAAGAGAGATAGAAGAAGTCACCTCGGAAGGGCATAACGAACCGATACCAAGATGGCTAGAAGAAGTCATCTCGGAAGGGCATAACGAACCCATACCAAGAGAGCGACAAGAAGCTATCTTGGAGGGGCACAGCATACCAATTCCAAGAGAGGTAATTATATTAGCCTTCTCATCAAGAATGATCAATGTGAAGATCGTGAAATGCCCTGCACTGAGTACGTTGCCAGGAGGCCTCCATCCACAGAAATTGGAAATAAGCGGGTGCAGTTCATTGAGTGCCCTCCCAGACGAAACCTGCGCAGGACGCTTGTTTCTTTTAACATTGGATTCATGTCAGGGTGACACATTACTGGGTATGATACCCAGTAGCCCCCAGTTACTATCTTTGATGATTTCAAATATCTCAGATGTCCGCTGCCTTCCAACTTTTCCCGGACTCCCCAAACTCCAAACGCTGCACATCCATGACTGTCCAGATCTCGAGTATCTCTCCACTGCAGAAAACAGGTTATTTGAACGCTTCACCTGTCTTCATATACTTTCCATCAAGAACTGTCTAAAGCTAACGACACTGCCAGCCGAAGGGCTCCCAACCAGTCTTTGGTATTTGAGTATCGGCCCGTGTGACTGCCTCAATTCATTTGGCCCCCCTGACGTACTCGTGAAACTCAACTCCCTCCGTGATCTTTACATTGAAGATTGCCCTGAACTCCAGTCCTTGCCAGACAATGGCTTGCCAGCCTCCCTCCTGCATCTGTCTATCAAAGGATGCCCCTCGCTGATGAAAAGCTGTGCAGAGGGTGGCAGCTATTGGCCAAAGATCAAGGACATCTCTGATCTGGAAATAGAAACACCAAGCTCATCTTCAAGCTCTGCTTGGTACAGTCTTTCTAGGAACCGCAGAGGACATAATTCCAAAGGAAAGAAGGTTCTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1343

Amino Acids

152.84

Weight (kDa)

5.44

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 98 8.2e-11 Rx N-terminal domain
NB-ARC PF00931 170 - 358 1.8e-34 NB-ARC domain
WHD_DRP PF23559 450 - 517 1.2e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 586 - 877 1.2e-22 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 704 - 844 7.4e-28 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1269, 1299
AasI GACNNNNNNGTC 1 cut(s) 1385
Acc16I TGCGCA 1 cut(s) 3391
Acc36I ACCTGC 2 cut(s) 1163, 3395
AccB7I CCANNNNNTGG 5 cut(s) 593, 1585, 2440, 3693, 3821
AccI GTMKAC 1 cut(s) 1377
AccII CGCG 2 cut(s) 1779, 2094
AciI CCGC 8 cut(s) 245, 1453, 1696, 2092, 2424, 3351, 3505, 3991
AclWI GGATC 4 cut(s) 176, 189, 2372, 3058
AcoI YGGCCR 3 cut(s) 1155, 2089, 3905
AcsI RAATTY 7 cut(s) 141, 718, 907, 1058, 2078, 2402, 2806
AcuI CTGAAG 6 cut(s) 224, 789, 1425, 1665, 1890, 2705
AdeI CACNNNGTG 1 cut(s) 2697
AflII CTTAAG 1 cut(s) 922
AflIII ACRYGT 1 cut(s) 2007
AgeI ACCGGT 1 cut(s) 451
AhlI ACTAGT 3 cut(s) 467, 631, 959
AjuI GAANNNNNNNTTGG 2 cut(s) 3940, 3972
AleI CACNNNNGTG 1 cut(s) 2775
AloI GAACNNNNNNTCC 1 cut(s) 4005
Alw21I GWGCWC 4 cut(s) 451, 1848, 2376, 3078
Alw26I GTCTC 2 cut(s) 1802, 1981
Alw44I GTGCAC 1 cut(s) 1844
AlwI GGATC 4 cut(s) 176, 189, 2372, 3058
AlwNI CAGNNNCTG 2 cut(s) 32, 991
Ama87I CYCGRG 2 cut(s) 1805, 3572
AoxI GGCC 9 cut(s) 1155, 1450, 2089, 2241, 2725, 3322, 3709, 3736, 3905
ApaLI GTGCAC 1 cut(s) 1844
ApeKI GCWGC 9 cut(s) 26, 233, 1093, 1657, 1693, 2661, 3507, 3547, 3897
ApoI RAATTY 7 cut(s) 141, 718, 907, 1058, 2078, 2402, 2806
ArsI GACNNNNNNTTYG 6 cut(s) 68, 100, 1159, 1191, 1962, 1994
AseI ATTAAT 1 cut(s) 1556
AsiGI ACCGGT 1 cut(s) 451
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 3 cut(s) 810, 1779, 3392
AspS9I GGNCC 4 cut(s) 126, 2726, 3710, 3737
AsuC2I CCSGG 6 cut(s) 2876, 2924, 2972, 3020, 3068, 3525
AsuHPI GGTGA 8 cut(s) 450, 967, 1040, 2239, 2789, 3103, 3443, 3607
AsuII TTCGAA 1 cut(s) 1491
AvaI CYCGRG 2 cut(s) 1805, 3572
AvaII GGWCC 1 cut(s) 126
BaeGI GKGCMC 5 cut(s) 1848, 2886, 2934, 3222, 3373
BaeI ACNNNNGTAYC 2 cut(s) 117, 150
BalI TGGCCA 2 cut(s) 1157, 3907
BamHI GGATCC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 2376, 3681
BarI GAAGNNNNNNTAC 2 cut(s) 2772, 2804
BauI CACGAG 1 cut(s) 3752
BbsI GAAGAC 5 cut(s) 174, 780, 2152, 2349, 3614
Bbv12I GWGCWC 4 cut(s) 451, 1848, 2376, 3078
BbvI GCAGC 9 cut(s) 38, 245, 1080, 1669, 1680, 2648, 3494, 3534, 3909
BccI CCATC 8 cut(s) 592, 1070, 1393, 2330, 2410, 3138, 3336, 3644
BceAI ACGGC 1 cut(s) 551
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciVI GTATCC 1 cut(s) 874
BclI TGATCA 1 cut(s) 3273
BcnI CCSGG 6 cut(s) 2876, 2924, 2972, 3020, 3068, 3525
BcoDI GTCTC 2 cut(s) 1802, 1981
BcuI ACTAGT 3 cut(s) 467, 631, 959
BfmI CTRYAG 3 cut(s) 1215, 2389, 3588
BfrI CTTAAG 1 cut(s) 922
BfuAI ACCTGC 2 cut(s) 1163, 3395
BfuI GTATCC 1 cut(s) 874
BglII AGATCT 1 cut(s) 3568
BmcAI AGTACT 2 cut(s) 193, 1638
Bme18I GGWCC 1 cut(s) 126
BmeT110I CYCGRG 2 cut(s) 1805, 3572
BmgT120I GGNCC 4 cut(s) 126, 2726, 3710, 3737
BmiI GGNNCC 7 cut(s) 6, 127, 183, 2352, 3680, 3739, 3989
BmrI ACTGGG 5 cut(s) 2109, 2782, 3450, 3452, 3460
BmsI GCATC 5 cut(s) 232, 814, 2033, 3851, 3853
BmuI ACTGGG 5 cut(s) 2109, 2782, 3450, 3452, 3460
BoxI GACNNNNGTC 1 cut(s) 1871
BpiI GAAGAC 5 cut(s) 174, 780, 2152, 2349, 3614
BplI GAGNNNNNCTC 2 cut(s) 3566, 3598
BpmI CTGGAG 5 cut(s) 2015, 2417, 2600, 2768, 3788
Bpu14I TTCGAA 1 cut(s) 1491
BpuEI CTTGAG 1 cut(s) 2555
BpuMI CCSGG 6 cut(s) 2876, 2924, 2972, 3020, 3068, 3525
BsaAI YACGTR 1 cut(s) 1774
BsaJI CCNNGG 7 cut(s) 102, 699, 970, 1789, 1940, 2360, 3114
BsaWI WCCGGW 1 cut(s) 451
Bse118I RCCGGY 1 cut(s) 451
Bse3DI GCAATG 2 cut(s) 1025, 2461
BseDI CCNNGG 7 cut(s) 102, 699, 970, 1789, 1940, 2360, 3114
BseMI GCAATG 2 cut(s) 1025, 2461
BseMII CTCAG 5 cut(s) 1413, 1976, 2626, 3296, 3510
BseRI GAGGAG 2 cut(s) 2252, 3086
BseSI GKGCMC 5 cut(s) 1848, 2886, 2934, 3222, 3373
BseXI GCAGC 9 cut(s) 38, 245, 1080, 1669, 1680, 2648, 3494, 3534, 3909
BseYI CCCAGC 1 cut(s) 121
BsgI GTGCAG 7 cut(s) 1159, 2048, 2223, 3285, 3376, 3533, 3906
Bsh1236I CGCG 2 cut(s) 1779, 2094
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 9 cut(s) 1157, 1452, 2091, 2243, 2727, 3324, 3711, 3738, 3907
BshTI ACCGGT 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 4 cut(s) 451, 1848, 2376, 3078
BsiHKCI CYCGRG 2 cut(s) 1805, 3572
BsiSI CCGG 7 cut(s) 452, 2876, 2924, 2972, 3020, 3068, 3525
BslFI GGGAC 4 cut(s) 139, 1443, 1551, 2554
BsmAI GTCTC 2 cut(s) 1802, 1981
BsmFI GGGAC 4 cut(s) 139, 1443, 1551, 2554
BsmI GAATGC 4 cut(s) 89, 1530, 2185, 2236
BsnI GGCC 9 cut(s) 1157, 1452, 2091, 2243, 2727, 3324, 3711, 3738, 3907
BsoBI CYCGRG 2 cut(s) 1805, 3572
Bsp119I TTCGAA 1 cut(s) 1491
Bsp1286I GDGCHC 9 cut(s) 451, 1848, 2376, 2886, 2934, 3078, 3222, 3373, 3681
BspACI CCGC 8 cut(s) 245, 1453, 1696, 2092, 2424, 3351, 3505, 3991
BspANI GGCC 9 cut(s) 1157, 1452, 2091, 2243, 2727, 3324, 3711, 3738, 3907
BspCNI CTCAG 5 cut(s) 1414, 1977, 2625, 3297, 3509
BspFNI CGCG 2 cut(s) 1779, 2094
BspHI TCATGA 1 cut(s) 1540
BspLI GGNNCC 7 cut(s) 6, 127, 183, 2352, 3680, 3739, 3989
BspMAI CTGCAG 1 cut(s) 3592
BspMI ACCTGC 2 cut(s) 1163, 3395
BspPI GGATC 4 cut(s) 176, 189, 2372, 3058
BspT104I TTCGAA 1 cut(s) 1491
BspTI CTTAAG 1 cut(s) 922
BsrDI GCAATG 2 cut(s) 1025, 2461
BsrFI RCCGGY 1 cut(s) 451
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 7 cut(s) 102, 699, 970, 1789, 1940, 2360, 3114
BssSI CACGAG 1 cut(s) 3752
BssT1I CCWWGG 3 cut(s) 102, 970, 2360
Bst2BI CACGAG 1 cut(s) 3752
Bst4CI ACNGT 7 cut(s) 1265, 1278, 1343, 2626, 3563, 3647, 3977
Bst6I CTCTTC 3 cut(s) 2550, 2589, 3042
BstAFI CTTAAG 1 cut(s) 922
BstAPI GCANNNNNTGC 1 cut(s) 991
BstBAI YACGTR 1 cut(s) 1774
BstBI TTCGAA 1 cut(s) 1491
BstC8I GCNNGC 9 cut(s) 45, 156, 1473, 1781, 2426, 2495, 3669, 3827, 3831
BstDEI CTNAG 7 cut(s) 1286, 1422, 1928, 1985, 2612, 3305, 3496
BstFNI CGCG 2 cut(s) 1779, 2094
BstHHI GCGC 3 cut(s) 810, 1779, 3392
BstMAI GTCTC 2 cut(s) 1802, 1981
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 6 cut(s) 26, 242, 991, 2180, 2240, 3396
BstNSI RCATGY 1 cut(s) 2011
BstPAI GACNNNNGTC 1 cut(s) 1871
BstSFI CTRYAG 3 cut(s) 1215, 2389, 3588
BstSLI GKGCMC 5 cut(s) 1848, 2886, 2934, 3222, 3373
BstUI CGCG 2 cut(s) 1779, 2094
BstV1I GCAGC 9 cut(s) 38, 245, 1080, 1669, 1680, 2648, 3494, 3534, 3909
BstV2I GAAGAC 5 cut(s) 174, 780, 2152, 2349, 3614
BstX2I RGATCY 2 cut(s) 181, 3568
BstYI RGATCY 2 cut(s) 181, 3568
BsuI GTATCC 1 cut(s) 874
BsuRI GGCC 9 cut(s) 1157, 1452, 2091, 2243, 2727, 3324, 3711, 3738, 3907
BtsI GCAGTG 3 cut(s) 1334, 3585, 3662
BtsIMutI CAGTG 9 cut(s) 47, 1334, 1746, 2206, 2631, 2770, 3302, 3585, 3662
BveI ACCTGC 2 cut(s) 1163, 3395
Cac8I GCNNGC 9 cut(s) 45, 156, 1473, 1781, 2426, 2495, 3669, 3827, 3831
CaiI CAGNNNCTG 2 cut(s) 32, 991
CciI TCATGA 1 cut(s) 1540
CfoI GCGC 3 cut(s) 810, 1779, 3392
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 4 cut(s) 126, 2726, 3710, 3737
CseI GACGC 3 cut(s) 816, 1520, 3405
CspAI ACCGGT 1 cut(s) 451
CspCI CAANNNNNGTGG 2 cut(s) 3321, 3356
DdeI CTNAG 7 cut(s) 1286, 1422, 1928, 1985, 2612, 3305, 3496
DraI TTTAAA 3 cut(s) 649, 912, 1054
DraIII CACNNNGTG 1 cut(s) 2697
DrdI GACNNNNNNGTC 1 cut(s) 1385
DseDI GACNNNNNNGTC 1 cut(s) 1385
EaeI YGGCCR 3 cut(s) 1155, 2089, 3905
Eam1104I CTCTTC 3 cut(s) 2550, 2589, 3042
EarI CTCTTC 3 cut(s) 2550, 2589, 3042
Ecl136II GAGCTC 1 cut(s) 2374
Eco130I CCWWGG 3 cut(s) 102, 970, 2360
Eco147I AGGCCT 1 cut(s) 3324
Eco24I GRGCYC 2 cut(s) 2376, 3681
Eco47I GGWCC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 2374
Eco57I CTGAAG 6 cut(s) 224, 789, 1425, 1665, 1890, 2705
Eco88I CYCGRG 2 cut(s) 1805, 3572
EcoICRI GAGCTC 1 cut(s) 2374
EcoT14I CCWWGG 3 cut(s) 102, 970, 2360
EcoT38I GRGCYC 2 cut(s) 2376, 3681
ErhI CCWWGG 3 cut(s) 102, 970, 2360
FaqI GGGAC 4 cut(s) 139, 1443, 1551, 2554
FauI CCCGC 2 cut(s) 2417, 3344
FbaI TGATCA 1 cut(s) 3273
FblI GTMKAC 1 cut(s) 1377
FriOI GRGCYC 2 cut(s) 2376, 3681
FspI TGCGCA 1 cut(s) 3391
GlaI GCGC 3 cut(s) 809, 1778, 3391
GsaI CCCAGC 1 cut(s) 125
GsuI CTGGAG 5 cut(s) 2015, 2417, 2600, 2768, 3788
HaeIII GGCC 9 cut(s) 1157, 1452, 2091, 2243, 2727, 3324, 3711, 3738, 3907
HapII CCGG 7 cut(s) 452, 2876, 2924, 2972, 3020, 3068, 3525
HgaI GACGC 3 cut(s) 816, 1520, 3405
HhaI GCGC 3 cut(s) 810, 1779, 3392
Hin6I GCGC 3 cut(s) 808, 1777, 3390
HinP1I GCGC 3 cut(s) 808, 1777, 3390
HincII GTYRAC 2 cut(s) 336, 1981
HindII GTYRAC 2 cut(s) 336, 1981
HindIII AAGCTT 2 cut(s) 41, 1838
HpaII CCGG 7 cut(s) 452, 2876, 2924, 2972, 3020, 3068, 3525
HphI GGTGA 8 cut(s) 450, 967, 1040, 2239, 2789, 3103, 3443, 3607
Hpy166II GTNNAC 8 cut(s) 336, 1148, 1321, 1378, 1763, 1771, 1846, 1981
Hpy8I GTNNAC 8 cut(s) 336, 1148, 1321, 1378, 1763, 1771, 1846, 1981
Hpy99I CGWCG 1 cut(s) 80
HpyCH4III ACNGT 7 cut(s) 1265, 1278, 1343, 2626, 3563, 3647, 3977
HpyCH4IV ACGT 4 cut(s) 1773, 1919, 3311, 3747
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 242, 991, 2180, 2240, 3396
HpyF3I CTNAG 7 cut(s) 1286, 1422, 1928, 1985, 2612, 3305, 3496
HpySE526I ACGT 4 cut(s) 1773, 1919, 3311, 3747
HspAI GCGC 3 cut(s) 808, 1777, 3390
Ksp22I TGATCA 1 cut(s) 3273
LmnI GCTCC 7 cut(s) 34, 1887, 1996, 2289, 2379, 3073, 3684
Lsp1109I GCAGC 9 cut(s) 38, 245, 1080, 1669, 1680, 2648, 3494, 3534, 3909
LweI GCATC 5 cut(s) 232, 814, 2033, 3851, 3853
MaeII ACGT 4 cut(s) 1773, 1919, 3311, 3747
MfeI CAATTG 1 cut(s) 588
MflI RGATCY 2 cut(s) 181, 3568
MhlI GDGCHC 9 cut(s) 451, 1848, 2376, 2886, 2934, 3078, 3222, 3373, 3681
MlsI TGGCCA 2 cut(s) 1157, 3907
MluNI TGGCCA 2 cut(s) 1157, 3907
MlyI GAGTC 7 cut(s) 491, 1229, 1814, 2452, 2481, 2576, 3522
MmeI TCCRAC 5 cut(s) 144, 783, 2393, 2520, 3539
Mox20I TGGCCA 2 cut(s) 1157, 3907
MroXI GAANNNNTTC 1 cut(s) 222
MscI TGGCCA 2 cut(s) 1157, 3907
MseI TTAA 9 cut(s) 648, 911, 923, 1053, 1181, 1556, 1667, 2802, 3410
MslI CAYNNNNRTG 3 cut(s) 1368, 2775, 3555
Msp20I TGGCCA 2 cut(s) 1157, 3907
MspA1I CMGCKG 1 cut(s) 3507
MspCI CTTAAG 1 cut(s) 922
MspI CCGG 7 cut(s) 452, 2876, 2924, 2972, 3020, 3068, 3525
MunI CAATTG 1 cut(s) 588
Mva1269I GAATGC 4 cut(s) 89, 1530, 2185, 2236
MvnI CGCG 2 cut(s) 1779, 2094
MwoI GCNNNNNNNGC 6 cut(s) 26, 242, 991, 2180, 2240, 3396
NciI CCSGG 6 cut(s) 2876, 2924, 2972, 3020, 3068, 3525
NlaIV GGNNCC 7 cut(s) 6, 127, 183, 2352, 3680, 3739, 3989
NmuCI GTSAC 8 cut(s) 709, 781, 955, 1028, 2384, 3109, 3431, 3716
NsbI TGCGCA 1 cut(s) 3391
NspI RCATGY 1 cut(s) 2011
NspV TTCGAA 1 cut(s) 1491
OliI CACNNNNGTG 1 cut(s) 2775
PaeR7I CTCGAG 2 cut(s) 1805, 3572
PagI TCATGA 1 cut(s) 1540
PceI AGGCCT 1 cut(s) 3324
PciI ACATGT 1 cut(s) 2007
PcsI WCGNNNNNNNCGW 2 cut(s) 75, 2322
PctI GAATGC 4 cut(s) 89, 1530, 2185, 2236
PdmI GAANNNNTTC 1 cut(s) 222
PflMI CCANNNNNTGG 5 cut(s) 593, 1585, 2440, 3693, 3821
PfoI TCCNGGA 7 cut(s) 2433, 2874, 2922, 2970, 3018, 3066, 3523
PinAI ACCGGT 1 cut(s) 451
Ple19I CGATCG 1 cut(s) 175
PleI GAGTC 7 cut(s) 490, 1228, 1814, 2452, 2480, 2575, 3522
PpsI GAGTC 7 cut(s) 490, 1228, 1814, 2452, 2480, 2575, 3522
Ppu21I YACGTR 1 cut(s) 1774
PscI ACATGT 1 cut(s) 2007
PshAI GACNNNNGTC 1 cut(s) 1871
PshBI ATTAAT 1 cut(s) 1556
PsiI TTATAA 2 cut(s) 1269, 1299
Psp124BI GAGCTC 1 cut(s) 2376
PspFI CCCAGC 1 cut(s) 121
PspN4I GGNNCC 7 cut(s) 6, 127, 183, 2352, 3680, 3739, 3989
PspPI GGNCC 4 cut(s) 126, 2726, 3710, 3737
PstI CTGCAG 1 cut(s) 3592
PstNI CAGNNNCTG 2 cut(s) 32, 991
PsuI RGATCY 2 cut(s) 181, 3568
PvuI CGATCG 1 cut(s) 175
RseI CAYNNNNRTG 3 cut(s) 1368, 2775, 3555
SacI GAGCTC 1 cut(s) 2376
SaqAI TTAA 9 cut(s) 648, 911, 923, 1053, 1181, 1556, 1667, 2802, 3410
Sau96I GGNCC 4 cut(s) 126, 2726, 3710, 3737
ScaI AGTACT 2 cut(s) 193, 1638
SchI GAGTC 7 cut(s) 491, 1229, 1814, 2452, 2481, 2576, 3522
SduI GDGCHC 9 cut(s) 451, 1848, 2376, 2886, 2934, 3078, 3222, 3373, 3681
SfaNI GCATC 5 cut(s) 232, 814, 2033, 3851, 3853
SfcI CTRYAG 3 cut(s) 1215, 2389, 3588
Sfr274I CTCGAG 2 cut(s) 1805, 3572
SfuI TTCGAA 1 cut(s) 1491
SinI GGWCC 1 cut(s) 126
SlaI CTCGAG 2 cut(s) 1805, 3572
SmiMI CAYNNNNRTG 3 cut(s) 1368, 2775, 3555
SmlI CTYRAG 4 cut(s) 922, 1805, 2534, 3572
SmoI CTYRAG 4 cut(s) 922, 1805, 2534, 3572
SpeI ACTAGT 3 cut(s) 467, 631, 959
SseBI AGGCCT 1 cut(s) 3324
SsiI CCGC 8 cut(s) 245, 1453, 1696, 2092, 2424, 3351, 3505, 3991
SstI GAGCTC 1 cut(s) 2376
StuI AGGCCT 1 cut(s) 3324
StyI CCWWGG 3 cut(s) 102, 970, 2360
TaaI ACNGT 7 cut(s) 1265, 1278, 1343, 2626, 3563, 3647, 3977
TaiI ACGT 4 cut(s) 1776, 1922, 3314, 3750
TatI WGTACW 5 cut(s) 191, 1273, 1496, 1518, 1636
TauI GCSGC 2 cut(s) 1455, 2094
Tru1I TTAA 9 cut(s) 648, 911, 923, 1053, 1181, 1556, 1667, 2802, 3410
Tru9I TTAA 9 cut(s) 648, 911, 923, 1053, 1181, 1556, 1667, 2802, 3410
TscAI CASTG 9 cut(s) 54, 1334, 1753, 2206, 2631, 2777, 3309, 3592, 3669
TseFI GTSAC 8 cut(s) 709, 781, 955, 1028, 2384, 3109, 3431, 3716
TseI GCWGC 9 cut(s) 26, 233, 1093, 1657, 1693, 2661, 3507, 3547, 3897
Tsp45I GTSAC 8 cut(s) 709, 781, 955, 1028, 2384, 3109, 3431, 3716
TspGWI ACGGA 2 cut(s) 2278, 3761
TspRI CASTG 9 cut(s) 54, 1334, 1753, 2206, 2631, 2777, 3309, 3592, 3669
Van91I CCANNNNNTGG 5 cut(s) 593, 1585, 2440, 3693, 3821
Vha464I CTTAAG 1 cut(s) 922
VneI GTGCAC 1 cut(s) 1844
VpaK11BI GGWCC 1 cut(s) 126
VspI ATTAAT 1 cut(s) 1556
XapI RAATTY 7 cut(s) 141, 718, 907, 1058, 2078, 2402, 2806
XceI RCATGY 1 cut(s) 2011
XcmI CCANNNNNNNNNTGG 2 cut(s) 1165, 3690
XhoI CTCGAG 2 cut(s) 1805, 3572
XmiI GTMKAC 1 cut(s) 1377
XmnI GAANNNNTTC 1 cut(s) 222
ZrmI AGTACT 2 cut(s) 193, 1638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.