Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0006459

Physical Location & Seq

Genomic Coordinates Forward (+)

25381297 .. 25388400

Gene Structure 7104 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g32330.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1914 bp

ATGGACACCATGATCTCGCTGAATAGGAGCTGCTTCCTGGGAAATCCTGGCGTATGTGGGAATGGAAGCCCGTTCTCTCAGAAGAGGCGGTCGGCGCCGCTGAGAGTGGTGGCGGTGGGGACGAACGCCGCCAGGAAAGGTGAAAGAAATGGGAGCATAGTGATGGAGACGGCAGCAAAAGAAGTTGTTGAGCTGAAATCTACCGTCCATGGTGGGGACAGAGACGTGTACGGTGAGGATACTGCCAGCGAGGACCAACCTGTTACACCTTGGTGTGTGTCTGTTGCAAGTGGTTATAATTTGATAAGAGATCCACACCACAACAAAGGTCTTGCCTTCTCTGAAAAGGAAAGAGATGCCCACTACTTGCGTGGTCTTCTCCCCCCAGTTGTTATTTCTCAAGAACTTCAGGTGAAGAAAATGATAAACGCCATCCGCCAGTATCAAGTTCCGCTGCAGAAGTATATAGCCATGATGGATCTTCAGGAAAGAAATCAGAAGCTATTTTACAAGCTTCTTATTGAGCATGTTGAGGAATTACTCCCAGTTGTTTATACTCCAACAGTTGGTGAAGCTTGCCAAAAATATGGAAGCATCTTCCAGCGTCCCCAGGGTCTATTCATAAGTTTGAAAGAAAAGGGAAAGATTCTTGAAGTGTTGCGGAACTGGCCAGAGAAGAATATTCAAGTCATAGTTGTCACTGACGGAGAGCGTATCCTAGGGCTGGGGGATCTTGGCTGTCATGGGATGGGTATACCTGTTGGCAAACTTTCTCTATATACAGCACTTGGAGGAGTTCGTCCATCTGCCTGCTTGCCTGTAACCATTGATGTGGGTACAAACAATGAGAATCTGCTGGACGATGAGTTCTACATAGGGCTCAGGCAAAAGAGGGCAACTGGGCAGGAATATGCAGAACTCCTTCAAGAATTCATGACTGCTGTCAAGCAGAATTATGGAGAGAAAGTTCTTGTACAGTTTGAGGACTTCGCAAACCACAATGCTTTTGATCTTCTTTCAAAGTATGGCGCAACTCATCTTGTTTTTAATGATGACATTCAGGGGACAGCATCTGTGGTTCTTGCAGGACTTATATCATCTTTGAAGTTAGTAGGGGGATCCTTAGCTGATCACACATTTTTATTCCTTGGTGCTGGAGAGGCTGGCACTGGCATTGCGGAGCTCATAGCCCTGGAAATATCTAAGCAGACAAATGCCCCAGTGGAAGAGACTCGCAAGAAGGTTTGGCTTGTGGACTCGAAGGGATTGATTGTCAGTTCGCGGTTGGAATCACTCCAACATTTTAAAAAGCCTTGGGCTCATGAACATGAACCGGTCACAACACTGGTCGATGCTGTTAATGCAATCAAGCCAACAGTGTTGATTGGAACTTCAGGAGTAGGAAGAACATTTACTAAAAAAGTAGTTGAGGCTATGGCCACCTACAATGAGAGACCCATTATCCTTGCTCTCTCCAACCCAACTTCACAATCTGAGTGTACTGCGGAAGAAGCATATACATGGACACAGGGTCGTGCCATTTACTGCAGTGGAAGCCCATTCCCACCAGTTGAATATGAGGGAAAGGTTTACACTCCTGGCCAGTCGAATAATGCCTACATCTTTCCTGGATTTGGTCTCGGCTTATTGATGTCTGGTACTATTCGTGTTCATGATGACATGCTTCTGGCAGCCTCGGAAGCTTTGGCTTCACAGGTATCCCAGGAAGATTTTGACAAGGGACTTATATACCCTCCATTTACAAACATCAGAGAGATTTCAGCACATATTGCTGCTAAAGTAGCTGCTAAATCATATGAACTCGGTTTGGCTACGCGCCTCCCCCAGCCAAAGGATCTGTTCCATTATGCTGAAAGCTGTATGTACAGCCCTAACTACCGAAGTTTCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006090 GO:0006108 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016053 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

638

Amino Acids

70.02

Weight (kDa)

6.24

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	22 - 202	6.8e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	213 - 464	5.9e-96	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0006459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79750
fragaria_vesca	FvH4_6g32330 FvH4_6g32330 FvH4_6g32330
malus_domestica	MD09G1203300.v1.1 MD17G1184200.v1.1
prunus_persica	Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1
pyrus_communis	pycom09g12010 pycom17g19390
rosa_chinensis	RchiOBHm_Chr2g0140441
rosa_laevigata	RLG00000019867
rosa_multiflora	Rmu_sc0003516.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436750 Rroxscaffold_2G00104830
rosa_rugosa	Rorug02G0362900 Rorug02G0363000 Rorug02G0363100 Rorug02G0363200 Rorug02G0363300
rosa_samantha	Rh2AG413500 Rh2BG423700 Rh2CG399700 Rh2DG433400
rosa_wichuraiana	Rw2G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	297
AccB1I	GGYRCC	1 cut(s)	94
AccB7I	CCANNNNNTGG	1 cut(s)	566
AccI	GTMKAC	1 cut(s)	754
AccII	CGCG	2 cut(s)	1282, 1837
AclWI	GGATC	6 cut(s)	305, 486, 738, 1113, 1126, 1863
AcoI	YGGCCR	3 cut(s)	668, 1437, 1600
AcsI	RAATTY	1 cut(s)	929
AcuI	CTGAAG	3 cut(s)	392, 467, 1377
AcyI	GRCGYC	1 cut(s)	95
AfaI	GTAC	6 cut(s)	230, 838, 975, 1501, 1660, 1886
AfiI	CCNNNNNNNGG	5 cut(s)	214, 566, 724, 1634, 1852
AflIII	ACRYGT	1 cut(s)	225
AgeI	ACCGGT	1 cut(s)	1333
AgsI	TTSAA	8 cut(s)	631, 653, 686, 926, 1020, 1105, 1574, 1909
AhdI	GACNNNNNGTC	1 cut(s)	1530
AjiI	CACGTC	1 cut(s)	226
AjnI	CCWGG	8 cut(s)	36, 46, 131, 609, 1191, 1597, 1627, 1722
AleI	CACNNNNGTG	1 cut(s)	271
AloI	GAACNNNNNNTCC	4 cut(s)	851, 883, 951, 983
Alw21I	GWGCWC	1 cut(s)	1185
Alw26I	GTCTC	5 cut(s)	161, 216, 1223, 1447, 1643
AlwI	GGATC	6 cut(s)	305, 486, 738, 1113, 1126, 1863
AlwNI	CAGNNNCTG	1 cut(s)	1073
AoxI	GGCC	3 cut(s)	668, 1437, 1600
ApeKI	GCWGC	6 cut(s)	30, 173, 454, 1691, 1793, 1805
ApoI	RAATTY	1 cut(s)	929
AsiGI	ACCGGT	1 cut(s)	1333
Asp700I	GAANNNNTTC	1 cut(s)	921
AspA2I	CCTAGG	1 cut(s)	718
AspLEI	GCGC	3 cut(s)	97, 1031, 1839
AspS9I	GGNCC	1 cut(s)	253
AsuHPI	GGTGA	4 cut(s)	152, 245, 424, 581
AvaII	GGWCC	1 cut(s)	253
AvrII	CCTAGG	1 cut(s)	718
BalI	TGGCCA	3 cut(s)	670, 1439, 1602
BamHI	GGATCC	1 cut(s)	1118
BanI	GGYRCC	1 cut(s)	94
BanII	GRGCYC	3 cut(s)	882, 1185, 1321
BarI	GAAGNNNNNNTAC	4 cut(s)	491, 523, 1396, 1428
BbsI	GAAGAC	1 cut(s)	368
Bbv12I	GWGCWC	1 cut(s)	1185
BbvI	GCAGC	6 cut(s)	17, 185, 441, 1703, 1780, 1792
BccI	CCATC	5 cut(s)	157, 440, 469, 742, 811
BceAI	ACGGC	1 cut(s)	186
BciT130I	CCWGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
BciVI	GTATCC	3 cut(s)	232, 725, 1729
BclI	TGATCA	1 cut(s)	1129
BcoDI	GTCTC	5 cut(s)	161, 216, 1223, 1447, 1643
BfaI	CTAG	1 cut(s)	719
BfmI	CTRYAG	2 cut(s)	455, 1546
BfoI	RGCGCY	1 cut(s)	98
BfuI	GTATCC	3 cut(s)	232, 725, 1729
BisI	GCNGC	8 cut(s)	31, 98, 129, 174, 455, 1692, 1794, 1806
BlnI	CCTAGG	1 cut(s)	718
BlsI	GCNGC	8 cut(s)	32, 99, 130, 175, 456, 1693, 1795, 1807
Bme1390I	CCNGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
Bme18I	GGWCC	1 cut(s)	253
BmeRI	GACNNNNNGTC	1 cut(s)	1530
BmgBI	CACGTC	1 cut(s)	226
BmgT120I	GGNCC	1 cut(s)	253
BmiI	GGNNCC	2 cut(s)	96, 1120
BmrFI	CCNGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
BmrI	ACTGGG	4 cut(s)	380, 539, 909, 1214
BmsI	GCATC	4 cut(s)	346, 603, 1079, 1342
BmuI	ACTGGG	4 cut(s)	380, 539, 909, 1214
BoxI	GACNNNNGTC	1 cut(s)	941
BpiI	GAAGAC	1 cut(s)	368
BpmI	CTGGAG	1 cut(s)	1176
Bpu10I	CCTNAGC	2 cut(s)	881, 1123
BpuEI	CTTGAG	1 cut(s)	384
BsaHI	GRCGYC	1 cut(s)	95
BsaI	GGTCTC	2 cut(s)	1447, 1643
BsaWI	WCCGGW	1 cut(s)	1333
BsaXI	ACNNNNNCTCC	2 cut(s)	951, 981
Bsc4I	CCNNNNNNNGG	5 cut(s)	214, 566, 724, 1634, 1852
Bse118I	RCCGGY	1 cut(s)	1333
Bse3DI	GCAATG	1 cut(s)	1173
BseBI	CCWGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
BseGI	GGATG	2 cut(s)	432, 753
BseLI	CCNNNNNNNGG	5 cut(s)	214, 566, 724, 1634, 1852
BseMI	GCAATG	1 cut(s)	1173
BseMII	CTCAG	4 cut(s)	92, 92, 895, 1485
BseRI	GAGGAG	1 cut(s)	807
BseXI	GCAGC	6 cut(s)	17, 185, 441, 1703, 1780, 1792
BseYI	CCCAGC	2 cut(s)	724, 1845
Bsh1236I	CGCG	2 cut(s)	1282, 1837
Bsh1285I	CGRYCG	1 cut(s)	92
BshFI	GGCC	3 cut(s)	670, 1439, 1602
BshNI	GGYRCC	1 cut(s)	94
BshTI	ACCGGT	1 cut(s)	1333
BsiEI	CGRYCG	1 cut(s)	92
BsiHKAI	GWGCWC	1 cut(s)	1185
BsiSI	CCGG	1 cut(s)	1334
BslFI	GGGAC	5 cut(s)	133, 230, 591, 1078, 1755
BslI	CCNNNNNNNGG	5 cut(s)	214, 566, 724, 1634, 1852
BsmAI	GTCTC	5 cut(s)	161, 216, 1223, 1447, 1643
BsmBI	CGTCTC	2 cut(s)	161, 216
BsmFI	GGGAC	5 cut(s)	133, 230, 591, 1078, 1755
BsnI	GGCC	3 cut(s)	670, 1439, 1602
Bso31I	GGTCTC	2 cut(s)	1447, 1643
Bsp1286I	GDGCHC	3 cut(s)	882, 1185, 1321
Bsp1407I	TGTACA	2 cut(s)	973, 1884
Bsp143I	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
Bsp19I	CCATGG	1 cut(s)	208
BspANI	GGCC	3 cut(s)	670, 1439, 1602
BspCNI	CTCAG	4 cut(s)	91, 93, 894, 1486
BspFNI	CGCG	2 cut(s)	1282, 1837
BspHI	TCATGA	3 cut(s)	933, 1321, 1672
BspLI	GGNNCC	2 cut(s)	96, 1120
BspMAI	CTGCAG	2 cut(s)	459, 1550
BspPI	GGATC	6 cut(s)	305, 486, 738, 1113, 1126, 1863
BspT107I	GGYRCC	1 cut(s)	94
BspTNI	GGTCTC	2 cut(s)	1447, 1643
BsrDI	GCAATG	1 cut(s)	1173
BsrFI	RCCGGY	1 cut(s)	1333
BsrGI	TGTACA	2 cut(s)	973, 1884
BssAI	RCCGGY	1 cut(s)	1333
BssMI	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
BssNAI	GTATAC	1 cut(s)	755
BssNI	GRCGYC	1 cut(s)	95
BssT1I	CCWWGG	5 cut(s)	208, 269, 718, 1147, 1313
Bst1107I	GTATAC	1 cut(s)	755
Bst2UI	CCWGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
Bst4CI	ACNGT	5 cut(s)	205, 233, 565, 978, 1378
Bst6I	CTCTTC	2 cut(s)	77, 1221
BstACI	GRCGYC	1 cut(s)	95
BstAPI	GCANNNNNTGC	1 cut(s)	1790
BstAUI	TGTACA	2 cut(s)	973, 1884
BstC8I	GCNNGC	5 cut(s)	247, 577, 811, 815, 1165
BstDEI	CTNAG	6 cut(s)	78, 101, 881, 1123, 1203, 1494
BstDSI	CCRYGG	1 cut(s)	208
BstF5I	GGATG	2 cut(s)	432, 753
BstFNI	CGCG	2 cut(s)	1282, 1837
BstH2I	RGCGCY	1 cut(s)	98
BstHHI	GCGC	3 cut(s)	97, 1031, 1839
BstKTI	GATC	8 cut(s)	15, 313, 481, 733, 1012, 1121, 1132, 1858
BstMAI	GTCTC	5 cut(s)	161, 216, 1223, 1447, 1643
BstMBI	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
BstMCI	CGRYCG	1 cut(s)	92
BstMWI	GCNNNNNNNGC	9 cut(s)	94, 667, 1160, 1361, 1511, 1554, 1700, 1790, 1802
BstNI	CCWGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
BstNSI	RCATGY	2 cut(s)	530, 1684
BstPAI	GACNNNNGTC	1 cut(s)	941
BstSCI	CCNGG	8 cut(s)	36, 46, 131, 609, 1191, 1597, 1627, 1722
BstSFI	CTRYAG	2 cut(s)	455, 1546
BstUI	CGCG	2 cut(s)	1282, 1837
BstV1I	GCAGC	6 cut(s)	17, 185, 441, 1703, 1780, 1792
BstV2I	GAAGAC	1 cut(s)	368
BstX2I	RGATCY	5 cut(s)	310, 478, 730, 1118, 1855
BstXI	CCANNNNNNTGG	2 cut(s)	587, 832
BstYI	RGATCY	5 cut(s)	310, 478, 730, 1118, 1855
BstZ17I	GTATAC	1 cut(s)	755
BsuI	GTATCC	3 cut(s)	232, 725, 1729
BsuRI	GGCC	3 cut(s)	670, 1439, 1602
BtgI	CCRYGG	1 cut(s)	208
BtrI	CACGTC	1 cut(s)	226
BtsCI	GGATG	2 cut(s)	432, 753
BtsI	GCAGTG	1 cut(s)	1555
BtsIMutI	CAGTG	6 cut(s)	699, 1167, 1227, 1343, 1383, 1555
Cac8I	GCNNGC	5 cut(s)	247, 577, 811, 815, 1165
CaiI	CAGNNNCTG	1 cut(s)	1073
CciI	TCATGA	3 cut(s)	933, 1321, 1672
CfoI	GCGC	3 cut(s)	97, 1031, 1839
Cfr10I	RCCGGY	1 cut(s)	1333
Cfr13I	GGNCC	1 cut(s)	253
CseI	GACGC	1 cut(s)	593
Csp6I	GTAC	6 cut(s)	229, 837, 974, 1500, 1659, 1885
CspAI	ACCGGT	1 cut(s)	1333
CviQI	GTAC	6 cut(s)	229, 837, 974, 1500, 1659, 1885
DdeI	CTNAG	6 cut(s)	78, 101, 881, 1123, 1203, 1494
DinI	GGCGCC	1 cut(s)	96
DpnI	GATC	8 cut(s)	14, 312, 480, 732, 1011, 1120, 1131, 1857
DpnII	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
DraI	TTTAAA	1 cut(s)	1306
DriI	GACNNNNNGTC	1 cut(s)	1530
EaeI	YGGCCR	3 cut(s)	668, 1437, 1600
Eam1104I	CTCTTC	2 cut(s)	77, 1221
Eam1105I	GACNNNNNGTC	1 cut(s)	1530
EarI	CTCTTC	2 cut(s)	77, 1221
EciI	GGCGGA	1 cut(s)	425
Ecl136II	GAGCTC	1 cut(s)	1183
Eco130I	CCWWGG	5 cut(s)	208, 269, 718, 1147, 1313
Eco24I	GRGCYC	3 cut(s)	882, 1185, 1321
Eco31I	GGTCTC	2 cut(s)	1447, 1643
Eco47I	GGWCC	1 cut(s)	253
Eco53kI	GAGCTC	1 cut(s)	1183
Eco57I	CTGAAG	3 cut(s)	392, 467, 1377
EcoICRI	GAGCTC	1 cut(s)	1183
EcoRI	GAATTC	1 cut(s)	929
EcoRII	CCWGG	8 cut(s)	36, 46, 131, 609, 1191, 1597, 1627, 1722
EcoT14I	CCWWGG	5 cut(s)	208, 269, 718, 1147, 1313
EcoT38I	GRGCYC	3 cut(s)	882, 1185, 1321
EgeI	GGCGCC	1 cut(s)	96
EheI	GGCGCC	1 cut(s)	96
ErhI	CCWWGG	5 cut(s)	208, 269, 718, 1147, 1313
Esp3I	CGTCTC	2 cut(s)	161, 216
FaqI	GGGAC	5 cut(s)	133, 230, 591, 1078, 1755
FauNDI	CATATG	1 cut(s)	1816
FbaI	TGATCA	1 cut(s)	1129
FblI	GTMKAC	1 cut(s)	754
Fnu4HI	GCNGC	8 cut(s)	31, 98, 129, 174, 455, 1692, 1794, 1806
FokI	GGATG	2 cut(s)	419, 760
FriOI	GRGCYC	3 cut(s)	882, 1185, 1321
Fsp4HI	GCNGC	8 cut(s)	31, 98, 129, 174, 455, 1692, 1794, 1806
FspBI	CTAG	1 cut(s)	719
GlaI	GCGC	3 cut(s)	96, 1030, 1838
GluI	GCNGC	8 cut(s)	31, 98, 129, 174, 455, 1692, 1794, 1806
GsaI	CCCAGC	2 cut(s)	728, 1849
GsuI	CTGGAG	1 cut(s)	1176
HaeII	RGCGCY	1 cut(s)	98
HaeIII	GGCC	3 cut(s)	670, 1439, 1602
HapII	CCGG	1 cut(s)	1334
HgaI	GACGC	1 cut(s)	593
HhaI	GCGC	3 cut(s)	97, 1031, 1839
Hin1I	GRCGYC	1 cut(s)	95
Hin6I	GCGC	3 cut(s)	95, 1029, 1837
HinP1I	GCGC	3 cut(s)	95, 1029, 1837
HindIII	AAGCTT	3 cut(s)	512, 573, 1701
HinfI	GANTC	5 cut(s)	646, 850, 1231, 1256, 1289
HpaII	CCGG	1 cut(s)	1334
HphI	GGTGA	4 cut(s)	152, 245, 424, 581
Hpy166II	GTNNAC	5 cut(s)	229, 755, 1255, 1500, 1591
Hpy188I	TCNGA	6 cut(s)	81, 343, 498, 1495, 1699, 1772
Hpy188III	TCNNGA	8 cut(s)	401, 485, 650, 926, 934, 1322, 1395, 1673
Hpy8I	GTNNAC	5 cut(s)	229, 755, 1255, 1500, 1591
HpyAV	CCTTC	4 cut(s)	346, 932, 1234, 1255
HpyCH4III	ACNGT	5 cut(s)	205, 233, 565, 978, 1378
HpyCH4IV	ACGT	1 cut(s)	225
HpyCH4V	TGCA	6 cut(s)	287, 457, 914, 1085, 1364, 1548
HpyF10VI	GCNNNNNNNGC	9 cut(s)	94, 667, 1160, 1361, 1511, 1554, 1700, 1790, 1802
HpyF3I	CTNAG	6 cut(s)	78, 101, 881, 1123, 1203, 1494
HpySE526I	ACGT	1 cut(s)	225
Hsp92I	GRCGYC	1 cut(s)	95
HspAI	GCGC	3 cut(s)	95, 1029, 1837
KasI	GGCGCC	1 cut(s)	94
Ksp22I	TGATCA	1 cut(s)	1129
Kzo9I	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
LmnI	GCTCC	3 cut(s)	27, 153, 1180
Lsp1109I	GCAGC	6 cut(s)	17, 185, 441, 1703, 1780, 1792
LweI	GCATC	4 cut(s)	346, 603, 1079, 1342
MaeI	CTAG	1 cut(s)	719
MaeII	ACGT	1 cut(s)	225
MaeIII	GTNAC	4 cut(s)	262, 697, 820, 1336
MalI	GATC	8 cut(s)	14, 312, 480, 732, 1011, 1120, 1131, 1857
MboI	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
MflI	RGATCY	5 cut(s)	310, 478, 730, 1118, 1855
MhlI	GDGCHC	3 cut(s)	882, 1185, 1321
MlsI	TGGCCA	3 cut(s)	670, 1439, 1602
MluCI	AATT	4 cut(s)	298, 536, 929, 952
MluNI	TGGCCA	3 cut(s)	670, 1439, 1602
Mly113I	GGCGCC	1 cut(s)	95
MlyI	GAGTC	2 cut(s)	1225, 1250
MmeI	TCCRAC	4 cut(s)	584, 1266, 1321, 1500
Mox20I	TGGCCA	3 cut(s)	670, 1439, 1602
MroXI	GAANNNNTTC	1 cut(s)	921
MscI	TGGCCA	3 cut(s)	670, 1439, 1602
MseI	TTAA	3 cut(s)	1047, 1305, 1359
MslI	CAYNNNNRTG	5 cut(s)	161, 271, 830, 1326, 1519
Msp20I	TGGCCA	3 cut(s)	670, 1439, 1602
MspA1I	CMGCKG	2 cut(s)	100, 454
MspI	CCGG	1 cut(s)	1334
MspR9I	CCNGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
MvaI	CCWGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
MvnI	CGCG	2 cut(s)	1282, 1837
MwoI	GCNNNNNNNGC	9 cut(s)	94, 667, 1160, 1361, 1511, 1554, 1700, 1790, 1802
NarI	GGCGCC	1 cut(s)	95
NcoI	CCATGG	1 cut(s)	208
NdeI	CATATG	1 cut(s)	1816
NdeII	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
NlaIV	GGNNCC	2 cut(s)	96, 1120
NmeAIII	GCCGAG	1 cut(s)	1620
NmuCI	GTSAC	2 cut(s)	697, 1336
NspI	RCATGY	2 cut(s)	530, 1684
OliI	CACNNNNGTG	1 cut(s)	271
PagI	TCATGA	3 cut(s)	933, 1321, 1672
PasI	CCCWGGG	1 cut(s)	610
PdmI	GAANNNNTTC	1 cut(s)	921
PfeI	GAWTC	3 cut(s)	646, 850, 1289
PflMI	CCANNNNNTGG	1 cut(s)	566
PfoI	TCCNGGA	1 cut(s)	1627
PinAI	ACCGGT	1 cut(s)	1333
PkrI	GCNGC	8 cut(s)	32, 99, 130, 175, 456, 1693, 1795, 1807
PleI	GAGTC	2 cut(s)	1225, 1250
PluTI	GGCGCC	1 cut(s)	98
PpsI	GAGTC	2 cut(s)	1225, 1250
PshAI	GACNNNNGTC	1 cut(s)	941
PsiI	TTATAA	1 cut(s)	297
Psp124BI	GAGCTC	1 cut(s)	1185
Psp6I	CCWGG	8 cut(s)	36, 46, 131, 609, 1191, 1597, 1627, 1722
PspFI	CCCAGC	2 cut(s)	724, 1845
PspGI	CCWGG	8 cut(s)	36, 46, 131, 609, 1191, 1597, 1627, 1722
PspN4I	GGNNCC	2 cut(s)	96, 1120
PspPI	GGNCC	1 cut(s)	253
PstI	CTGCAG	2 cut(s)	459, 1550
PstNI	CAGNNNCTG	1 cut(s)	1073
PsuI	RGATCY	5 cut(s)	310, 478, 730, 1118, 1855
RsaI	GTAC	6 cut(s)	230, 838, 975, 1501, 1660, 1886
RsaNI	GTAC	6 cut(s)	229, 837, 974, 1500, 1659, 1885
RseI	CAYNNNNRTG	5 cut(s)	161, 271, 830, 1326, 1519
SacI	GAGCTC	1 cut(s)	1185
SaqAI	TTAA	3 cut(s)	1047, 1305, 1359
SatI	GCNGC	8 cut(s)	31, 98, 129, 174, 455, 1692, 1794, 1806
Sau3AI	GATC	8 cut(s)	12, 310, 478, 730, 1009, 1118, 1129, 1855
Sau96I	GGNCC	1 cut(s)	253
SchI	GAGTC	2 cut(s)	1225, 1250
ScrFI	CCNGG	8 cut(s)	38, 48, 133, 611, 1193, 1599, 1629, 1724
SduI	GDGCHC	3 cut(s)	882, 1185, 1321
SfaNI	GCATC	4 cut(s)	346, 603, 1079, 1342
SfcI	CTRYAG	2 cut(s)	455, 1546
SfoI	GGCGCC	1 cut(s)	96
SinI	GGWCC	1 cut(s)	253
SmiMI	CAYNNNNRTG	5 cut(s)	161, 271, 830, 1326, 1519
SmlI	CTYRAG	1 cut(s)	399
SmoI	CTYRAG	1 cut(s)	399
Sse9I	AATT	4 cut(s)	298, 536, 929, 952
SspDI	GGCGCC	1 cut(s)	94
SspI	AATATT	1 cut(s)	682
SspMI	CTAG	1 cut(s)	719
SstI	GAGCTC	1 cut(s)	1185
StyD4I	CCNGG	8 cut(s)	36, 46, 131, 609, 1191, 1597, 1627, 1722
StyI	CCWWGG	5 cut(s)	208, 269, 718, 1147, 1313
TaaI	ACNGT	5 cut(s)	205, 233, 565, 978, 1378
TaiI	ACGT	1 cut(s)	228
TaqI	TCGA	3 cut(s)	1259, 1350, 1607
TasI	AATT	4 cut(s)	298, 536, 929, 952
TatI	WGTACW	3 cut(s)	973, 1499, 1884
TauI	GCSGC	2 cut(s)	100, 131
TfiI	GAWTC	3 cut(s)	646, 850, 1289
Tru1I	TTAA	3 cut(s)	1047, 1305, 1359
Tru9I	TTAA	3 cut(s)	1047, 1305, 1359
TscAI	CASTG	6 cut(s)	706, 1174, 1227, 1350, 1383, 1555
TseFI	GTSAC	2 cut(s)	697, 1336
TseI	GCWGC	6 cut(s)	30, 173, 454, 1691, 1793, 1805
Tsp45I	GTSAC	2 cut(s)	697, 1336
TspDTI	ATGAA	6 cut(s)	610, 922, 1338, 1344, 1661, 1833
TspGWI	ACGGA	1 cut(s)	720
TspRI	CASTG	6 cut(s)	706, 1174, 1227, 1350, 1383, 1555
Van91I	CCANNNNNTGG	1 cut(s)	566
VpaK11BI	GGWCC	1 cut(s)	253
XapI	RAATTY	1 cut(s)	929
XceI	RCATGY	2 cut(s)	530, 1684
XcmI	CCANNNNNNNNNTGG	1 cut(s)	368
XmaJI	CCTAGG	1 cut(s)	718
XmiI	GTMKAC	1 cut(s)	754
XmnI	GAANNNNTTC	1 cut(s)	921
XspI	CTAG	1 cut(s)	719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.