Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0006459

Physical Location & Seq

Genomic Coordinates Reverse (-)

59664787 .. 59671115

Gene Structure 6329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG423700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGATCTCGCTGAATAGAAGCTGCTTCCTGAGCAACGGGGGCATTTCTGGGAATTGTAGCCCGTTCTCTCAGGGGCAGAAGAGGCGGTCGGCTTCGCTGAAAGTGGTGGCTGTAGGGACGAACGCCGCCAGGAAAGGTGAACGAAACGGCAGCATAGTGATGGAGAACGCAGCAAAAGAAGTTGTTGAGCTGAAATCTACCGTCCATGGTGGGGACAAAGACGTGTATGGTGAGGATACTGCCAGCGAGGACCAACCTGTCACGCCCTGGAGTGTGTCTGTTGCAAGTGGTTATAATTTGATACGAGATCCACACCACAACAAAGGTCTTGCCTTCTCTGAAAAGGAGAGAGATGCCCACTACATGCGTGGTCTTCTTCCCCCAGTTGTTATTTCTCAAGAACTTCAGGTGAAGAAAATGATAAACGCCATCCGCCAGTATCAAGTTCCCCTGCAGAAGTATATAGCCATGATGGATCTTCAGGAAAGAAACCAGAAGCTATTCTACAAGCTTCTTATCGAGCATGTTGAGGAATTACTCCCAGTTGTTTACACTCCAACCGTCGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCCAGCGTCCCCAGGGTCTATTCATAAGTTTGAAAGAAAAGGGAAAGATTCTTGAAGTGTTGCGGAACTGGCCAGAGAAGAATATTCAAGTCATTGTCGTCACTGATGGAGAGCGTATCCTAGGGCTGGGGGATCTTGGCTGCCATGGGATGGGTATACCTGTAGGCAAACTTTCTCTATATACGGCACTTGGAGGAGTTCGTCCATCTGCTTGCTTGCCTGTAACCATTGATGTGGGTACAAACAATGAGAAGCTGTTGGATGATGAGTTCTACATAGGGCTCAGGCAAAAGAGGGCAACTGGGCAGGAATACGCAGAACTCCTTCAAGAATTCATGACTGCTGTGAAGCAGAATTATGGAGAGAAAGTTCTTGTTCAGTTTGAGGACTTTGCAAACCACAATGCTTTTGATCTTCTTTCGAAGTATGGTGCAACTCATCTTGTTTTTAATGATGATATTCAGGGGACAGCATCTGTGGTCCTTGCAGGACTCTTGTCATCTTTGAAGTTAGTAGGGGGATCCTTAGCTGATCACACATTTTTATTCCTTGGTGCTGGAGAGGCTGGCACTGGCATAGCGGAGCTCATAGCCCTTGAAATATCTAAGCAGACAAATGCCCCACTGGAAGAAACTCGCAAGAAGGTTTGGCTTGTTGACTCGAAGGGATTGATTGTCAGTTCCCGGTTGGAATCACTCCAACATTTTAAGAAGCCTTGGGCTCATGAACATGAACCAGTCAAGACACTTGCAGAAGCTGTTAATGCAATCAAGCCAACGGTGTTGATTGGAACGTCAGGAGTAGGAAGAACATTTACTAAAGAAGTGGTTGAGGCTATGGCCACCAACAATGAGGTATACTATTCCCTATATGTGTGTGTGTGTTTTGGGTTGTTCTATGAGCATGAACAGCATCCATACATGATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006090 GO:0006108 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016053 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

507

Amino Acids

55.99

Weight (kDa)

6.42

Isoelectric Point (pI)

34.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	161 - 341	4.6e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	352 - 483	1.4e-39	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79750
fragaria_vesca	FvH4_6g32330 FvH4_6g32330 FvH4_6g32330
malus_domestica	MD09G1203300.v1.1 MD17G1184200.v1.1
prunus_persica	Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1
pyrus_communis	pycom09g12010 pycom17g19390
rosa_chinensis	RchiOBHm_Chr2g0140441
rosa_laevigata	RLG00000019867
rosa_multiflora	Rmu_sc0003516.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436750 Rroxscaffold_2G00104830
rosa_rugosa	Rorug02G0362900 Rorug02G0363000 Rorug02G0363100 Rorug02G0363200 Rorug02G0363300
rosa_samantha	Rh2AG413500 Rh2BG423700 Rh2CG399700 Rh2DG433400
rosa_wichuraiana	Rw2G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	294
AasI	GACNNNNNNGTC	1 cut(s)	257
AccB7I	CCANNNNNTGG	1 cut(s)	745
AccI	GTMKAC	2 cut(s)	751, 1452
AciI	CCGC	5 cut(s)	85, 126, 433, 658, 1175
AclWI	GGATC	5 cut(s)	302, 483, 735, 1110, 1123
AcoI	YGGCCR	2 cut(s)	665, 1434
AcsI	RAATTY	1 cut(s)	926
AcuI	CTGAAG	2 cut(s)	389, 464
AfaI	GTAC	1 cut(s)	835
AfiI	CCNNNNNNNGG	4 cut(s)	211, 563, 721, 745
AflIII	ACRYGT	1 cut(s)	222
AgsI	TTSAA	6 cut(s)	628, 650, 683, 923, 1102, 1193
AjiI	CACGTC	1 cut(s)	223
AjnI	CCWGG	3 cut(s)	128, 266, 606
AloI	GAACNNNNNNTCC	4 cut(s)	848, 880, 948, 980
AluBI	AGCT	9 cut(s)	21, 190, 499, 511, 572, 850, 1124, 1180, 1352
AluI	AGCT	9 cut(s)	21, 190, 499, 511, 572, 850, 1124, 1180, 1352
Alw21I	GWGCWC	1 cut(s)	1182
AlwI	GGATC	5 cut(s)	302, 483, 735, 1110, 1123
AlwNI	CAGNNNCTG	2 cut(s)	1070, 1352
AoxI	GGCC	2 cut(s)	665, 1434
ApeKI	GCWGC	4 cut(s)	21, 150, 170, 735
ApoI	RAATTY	1 cut(s)	926
Asp700I	GAANNNNTTC	2 cut(s)	500, 918
AspA2I	CCTAGG	1 cut(s)	715
AspS9I	GGNCC	2 cut(s)	250, 1075
AsuC2I	CCSGG	1 cut(s)	1279
AsuHPI	GGTGA	4 cut(s)	149, 242, 421, 578
AsuII	TTCGAA	1 cut(s)	1016
AvaII	GGWCC	2 cut(s)	250, 1075
AvrII	CCTAGG	1 cut(s)	715
BalI	TGGCCA	2 cut(s)	667, 1436
BamHI	GGATCC	1 cut(s)	1115
BanII	GRGCYC	3 cut(s)	879, 1182, 1318
BarI	GAAGNNNNNNTAC	4 cut(s)	488, 520, 1393, 1425
BbsI	GAAGAC	1 cut(s)	365
Bbv12I	GWGCWC	1 cut(s)	1182
BbvI	GCAGC	4 cut(s)	8, 162, 182, 722
BccI	CCATC	6 cut(s)	154, 437, 466, 695, 739, 808
BceAI	ACGGC	2 cut(s)	163, 795
BciT130I	CCWGG	3 cut(s)	130, 268, 608
BciVI	GTATCC	2 cut(s)	229, 722
BclI	TGATCA	1 cut(s)	1126
BcnI	CCSGG	1 cut(s)	1279
BfaI	CTAG	1 cut(s)	716
BfmI	CTRYAG	3 cut(s)	111, 452, 756
BfuI	GTATCC	2 cut(s)	229, 722
BisI	GCNGC	5 cut(s)	22, 126, 151, 171, 736
BlnI	CCTAGG	1 cut(s)	715
BlsI	GCNGC	5 cut(s)	23, 127, 152, 172, 737
Bme1390I	CCNGG	4 cut(s)	130, 268, 608, 1279
Bme18I	GGWCC	2 cut(s)	250, 1075
BmgBI	CACGTC	1 cut(s)	223
BmgT120I	GGNCC	2 cut(s)	250, 1075
BmiI	GGNNCC	1 cut(s)	1117
BmrFI	CCNGG	4 cut(s)	130, 268, 608, 1279
BmrI	ACTGGG	3 cut(s)	377, 536, 906
BmsI	GCATC	4 cut(s)	343, 600, 1076, 1516
BmuI	ACTGGG	3 cut(s)	377, 536, 906
BoxI	GACNNNNGTC	1 cut(s)	1090
BpiI	GAAGAC	1 cut(s)	365
BpmI	CTGGAG	2 cut(s)	289, 1173
Bpu10I	CCTNAGC	3 cut(s)	29, 878, 1120
Bpu14I	TTCGAA	1 cut(s)	1016
BpuEI	CTTGAG	1 cut(s)	381
BpuMI	CCSGG	1 cut(s)	1279
BsaJI	CCNNGG	8 cut(s)	205, 266, 606, 607, 715, 739, 1144, 1310
BsaXI	ACNNNNNCTCC	2 cut(s)	948, 978
Bsc4I	CCNNNNNNNGG	4 cut(s)	211, 563, 721, 745
Bse1I	ACTGG	8 cut(s)	383, 436, 542, 668, 901, 1171, 1224, 1331
BseBI	CCWGG	3 cut(s)	130, 268, 608
BseDI	CCNNGG	8 cut(s)	205, 266, 606, 607, 715, 739, 1144, 1310
BseGI	GGATG	4 cut(s)	429, 750, 862, 1507
BseLI	CCNNNNNNNGG	4 cut(s)	211, 563, 721, 745
BseMII	CTCAG	3 cut(s)	20, 83, 892
BseNI	ACTGG	8 cut(s)	383, 436, 542, 668, 901, 1171, 1224, 1331
BseRI	GAGGAG	1 cut(s)	804
BseXI	GCAGC	4 cut(s)	8, 162, 182, 722
BseYI	CCCAGC	1 cut(s)	721
Bsh1285I	CGRYCG	1 cut(s)	89
BshFI	GGCC	2 cut(s)	667, 1436
BsiEI	CGRYCG	1 cut(s)	89
BsiHKAI	GWGCWC	1 cut(s)	1182
BsiSI	CCGG	1 cut(s)	1279
BslFI	GGGAC	4 cut(s)	130, 227, 588, 1075
BslI	CCNNNNNNNGG	4 cut(s)	211, 563, 721, 745
BsmFI	GGGAC	4 cut(s)	130, 227, 588, 1075
BsnI	GGCC	2 cut(s)	667, 1436
Bsp119I	TTCGAA	1 cut(s)	1016
Bsp1286I	GDGCHC	3 cut(s)	879, 1182, 1318
Bsp143I	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
Bsp19I	CCATGG	2 cut(s)	205, 739
BspACI	CCGC	5 cut(s)	85, 126, 433, 658, 1175
BspANI	GGCC	2 cut(s)	667, 1436
BspCNI	CTCAG	3 cut(s)	21, 82, 891
BspHI	TCATGA	2 cut(s)	930, 1318
BspLI	GGNNCC	1 cut(s)	1117
BspMAI	CTGCAG	1 cut(s)	456
BspPI	GGATC	5 cut(s)	302, 483, 735, 1110, 1123
BspT104I	TTCGAA	1 cut(s)	1016
BsrI	ACTGG	8 cut(s)	383, 436, 542, 668, 901, 1171, 1224, 1331
BssECI	CCNNGG	8 cut(s)	205, 266, 606, 607, 715, 739, 1144, 1310
BssMI	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
BssNAI	GTATAC	2 cut(s)	752, 1453
BssT1I	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
Bst1107I	GTATAC	2 cut(s)	752, 1453
Bst2UI	CCWGG	3 cut(s)	130, 268, 608
Bst4CI	ACNGT	3 cut(s)	202, 562, 1375
Bst6I	CTCTTC	1 cut(s)	74
BstBI	TTCGAA	1 cut(s)	1016
BstC8I	GCNNGC	5 cut(s)	244, 574, 808, 812, 1162
BstDEI	CTNAG	5 cut(s)	29, 69, 878, 1120, 1200
BstDSI	CCRYGG	2 cut(s)	205, 739
BstF5I	GGATG	4 cut(s)	429, 750, 862, 1507
BstKTI	GATC	7 cut(s)	6, 310, 478, 730, 1009, 1118, 1129
BstMBI	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
BstMCI	CGRYCG	1 cut(s)	89
BstMWI	GCNNNNNNNGC	7 cut(s)	30, 39, 82, 664, 1157, 1358, 1504
BstNI	CCWGG	3 cut(s)	130, 268, 608
BstNSI	RCATGY	2 cut(s)	367, 527
BstPAI	GACNNNNGTC	1 cut(s)	1090
BstSCI	CCNGG	4 cut(s)	128, 266, 606, 1277
BstSFI	CTRYAG	3 cut(s)	111, 452, 756
BstV1I	GCAGC	4 cut(s)	8, 162, 182, 722
BstV2I	GAAGAC	1 cut(s)	365
BstX2I	RGATCY	4 cut(s)	307, 475, 727, 1115
BstXI	CCANNNNNNTGG	2 cut(s)	584, 829
BstYI	RGATCY	4 cut(s)	307, 475, 727, 1115
BstZ17I	GTATAC	2 cut(s)	752, 1453
BsuI	GTATCC	2 cut(s)	229, 722
BsuRI	GGCC	2 cut(s)	667, 1436
BtgI	CCRYGG	2 cut(s)	205, 739
BtrI	CACGTC	1 cut(s)	223
BtsCI	GGATG	4 cut(s)	429, 750, 862, 1507
BtsIMutI	CAGTG	3 cut(s)	696, 1164, 1217
Cac8I	GCNNGC	5 cut(s)	244, 574, 808, 812, 1162
CaiI	CAGNNNCTG	2 cut(s)	1070, 1352
CciI	TCATGA	2 cut(s)	930, 1318
Cfr13I	GGNCC	2 cut(s)	250, 1075
CseI	GACGC	1 cut(s)	590
Csp6I	GTAC	1 cut(s)	834
CviQI	GTAC	1 cut(s)	834
DdeI	CTNAG	5 cut(s)	29, 69, 878, 1120, 1200
DpnI	GATC	7 cut(s)	5, 309, 477, 729, 1008, 1117, 1128
DpnII	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
DrdI	GACNNNNNNGTC	1 cut(s)	257
DseDI	GACNNNNNNGTC	1 cut(s)	257
EaeI	YGGCCR	2 cut(s)	665, 1434
Eam1104I	CTCTTC	1 cut(s)	74
EarI	CTCTTC	1 cut(s)	74
EciI	GGCGGA	1 cut(s)	422
Ecl136II	GAGCTC	1 cut(s)	1180
Eco130I	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
Eco24I	GRGCYC	3 cut(s)	879, 1182, 1318
Eco47I	GGWCC	2 cut(s)	250, 1075
Eco53kI	GAGCTC	1 cut(s)	1180
Eco57I	CTGAAG	2 cut(s)	389, 464
EcoICRI	GAGCTC	1 cut(s)	1180
EcoRI	GAATTC	1 cut(s)	926
EcoRII	CCWGG	3 cut(s)	128, 266, 606
EcoT14I	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
EcoT38I	GRGCYC	3 cut(s)	879, 1182, 1318
ErhI	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
FaqI	GGGAC	4 cut(s)	130, 227, 588, 1075
FbaI	TGATCA	1 cut(s)	1126
FblI	GTMKAC	2 cut(s)	751, 1452
Fnu4HI	GCNGC	5 cut(s)	22, 126, 151, 171, 736
FokI	GGATG	4 cut(s)	416, 757, 869, 1494
FriOI	GRGCYC	3 cut(s)	879, 1182, 1318
Fsp4HI	GCNGC	5 cut(s)	22, 126, 151, 171, 736
FspBI	CTAG	1 cut(s)	716
GluI	GCNGC	5 cut(s)	22, 126, 151, 171, 736
GsaI	CCCAGC	1 cut(s)	725
GsuI	CTGGAG	2 cut(s)	289, 1173
HaeIII	GGCC	2 cut(s)	667, 1436
HapII	CCGG	1 cut(s)	1279
HgaI	GACGC	1 cut(s)	590
HincII	GTYRAC	1 cut(s)	1252
HindII	GTYRAC	1 cut(s)	1252
HindIII	AAGCTT	2 cut(s)	509, 570
HinfI	GANTC	4 cut(s)	643, 1086, 1253, 1286
HpaII	CCGG	1 cut(s)	1279
HphI	GGTGA	4 cut(s)	149, 242, 421, 578
Hpy166II	GTNNAC	5 cut(s)	140, 550, 752, 1252, 1453
Hpy188I	TCNGA	1 cut(s)	340
Hpy188III	TCNNGA	9 cut(s)	28, 398, 482, 647, 923, 931, 1319, 1336, 1392
Hpy8I	GTNNAC	5 cut(s)	140, 550, 752, 1252, 1453
Hpy99I	CGWCG	1 cut(s)	566
HpyAV	CCTTC	4 cut(s)	343, 929, 1231, 1252
HpyCH4III	ACNGT	3 cut(s)	202, 562, 1375
HpyCH4IV	ACGT	2 cut(s)	222, 1388
HpyCH4V	TGCA	7 cut(s)	284, 454, 989, 1028, 1082, 1346, 1361
HpyF10VI	GCNNNNNNNGC	7 cut(s)	30, 39, 82, 664, 1157, 1358, 1504
HpyF3I	CTNAG	5 cut(s)	29, 69, 878, 1120, 1200
HpySE526I	ACGT	2 cut(s)	222, 1388
Ksp22I	TGATCA	1 cut(s)	1126
Kzo9I	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
LmnI	GCTCC	1 cut(s)	1177
Lsp1109I	GCAGC	4 cut(s)	8, 162, 182, 722
LweI	GCATC	4 cut(s)	343, 600, 1076, 1516
MaeI	CTAG	1 cut(s)	716
MaeII	ACGT	2 cut(s)	222, 1388
MaeIII	GTNAC	3 cut(s)	259, 694, 817
MalI	GATC	7 cut(s)	5, 309, 477, 729, 1008, 1117, 1128
MboI	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
MflI	RGATCY	4 cut(s)	307, 475, 727, 1115
MhlI	GDGCHC	3 cut(s)	879, 1182, 1318
MlsI	TGGCCA	2 cut(s)	667, 1436
MluCI	AATT	5 cut(s)	52, 295, 533, 926, 949
MluNI	TGGCCA	2 cut(s)	667, 1436
MlyI	GAGTC	2 cut(s)	1080, 1247
MmeI	TCCRAC	4 cut(s)	581, 834, 1263, 1318
Mox20I	TGGCCA	2 cut(s)	667, 1436
MroXI	GAANNNNTTC	2 cut(s)	500, 918
MscI	TGGCCA	2 cut(s)	667, 1436
MseI	TTAA	3 cut(s)	1044, 1302, 1356
MslI	CAYNNNNRTG	3 cut(s)	158, 827, 1323
Msp20I	TGGCCA	2 cut(s)	667, 1436
MspI	CCGG	1 cut(s)	1279
MspR9I	CCNGG	4 cut(s)	130, 268, 608, 1279
MvaI	CCWGG	3 cut(s)	130, 268, 608
MwoI	GCNNNNNNNGC	7 cut(s)	30, 39, 82, 664, 1157, 1358, 1504
NciI	CCSGG	1 cut(s)	1279
NcoI	CCATGG	2 cut(s)	205, 739
NdeII	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
NlaIV	GGNNCC	1 cut(s)	1117
NmuCI	GTSAC	2 cut(s)	259, 694
NspI	RCATGY	2 cut(s)	367, 527
NspV	TTCGAA	1 cut(s)	1016
PagI	TCATGA	2 cut(s)	930, 1318
PasI	CCCWGGG	1 cut(s)	607
PdmI	GAANNNNTTC	2 cut(s)	500, 918
PfeI	GAWTC	2 cut(s)	643, 1286
PflMI	CCANNNNNTGG	1 cut(s)	745
PkrI	GCNGC	5 cut(s)	23, 127, 152, 172, 737
PleI	GAGTC	2 cut(s)	1080, 1247
PpsI	GAGTC	2 cut(s)	1080, 1247
PshAI	GACNNNNGTC	1 cut(s)	1090
PsiI	TTATAA	1 cut(s)	294
Psp124BI	GAGCTC	1 cut(s)	1182
Psp6I	CCWGG	3 cut(s)	128, 266, 606
PspFI	CCCAGC	1 cut(s)	721
PspGI	CCWGG	3 cut(s)	128, 266, 606
PspN4I	GGNNCC	1 cut(s)	1117
PspPI	GGNCC	2 cut(s)	250, 1075
PstI	CTGCAG	1 cut(s)	456
PstNI	CAGNNNCTG	2 cut(s)	1070, 1352
PsuI	RGATCY	4 cut(s)	307, 475, 727, 1115
RsaI	GTAC	1 cut(s)	835
RsaNI	GTAC	1 cut(s)	834
RseI	CAYNNNNRTG	3 cut(s)	158, 827, 1323
SacI	GAGCTC	1 cut(s)	1182
SaqAI	TTAA	3 cut(s)	1044, 1302, 1356
SatI	GCNGC	5 cut(s)	22, 126, 151, 171, 736
Sau3AI	GATC	7 cut(s)	3, 307, 475, 727, 1006, 1115, 1126
Sau96I	GGNCC	2 cut(s)	250, 1075
SchI	GAGTC	2 cut(s)	1080, 1247
ScrFI	CCNGG	4 cut(s)	130, 268, 608, 1279
SduI	GDGCHC	3 cut(s)	879, 1182, 1318
SfaNI	GCATC	4 cut(s)	343, 600, 1076, 1516
SfcI	CTRYAG	3 cut(s)	111, 452, 756
SfuI	TTCGAA	1 cut(s)	1016
SinI	GGWCC	2 cut(s)	250, 1075
SmiMI	CAYNNNNRTG	3 cut(s)	158, 827, 1323
SmlI	CTYRAG	1 cut(s)	396
SmoI	CTYRAG	1 cut(s)	396
Sse9I	AATT	5 cut(s)	52, 295, 533, 926, 949
SsiI	CCGC	5 cut(s)	85, 126, 433, 658, 1175
SspI	AATATT	1 cut(s)	679
SspMI	CTAG	1 cut(s)	716
SstI	GAGCTC	1 cut(s)	1182
StyD4I	CCNGG	4 cut(s)	128, 266, 606, 1277
StyI	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
TaaI	ACNGT	3 cut(s)	202, 562, 1375
TaiI	ACGT	2 cut(s)	225, 1391
TaqI	TCGA	3 cut(s)	519, 1016, 1256
TasI	AATT	5 cut(s)	52, 295, 533, 926, 949
TauI	GCSGC	1 cut(s)	128
TfiI	GAWTC	2 cut(s)	643, 1286
Tru1I	TTAA	3 cut(s)	1044, 1302, 1356
Tru9I	TTAA	3 cut(s)	1044, 1302, 1356
TscAI	CASTG	3 cut(s)	703, 1171, 1224
TseFI	GTSAC	2 cut(s)	259, 694
TseI	GCWGC	4 cut(s)	21, 150, 170, 735
Tsp45I	GTSAC	2 cut(s)	259, 694
TspDTI	ATGAA	5 cut(s)	607, 919, 1335, 1341, 1515
TspRI	CASTG	3 cut(s)	703, 1171, 1224
Van91I	CCANNNNNTGG	1 cut(s)	745
VpaK11BI	GGWCC	2 cut(s)	250, 1075
XapI	RAATTY	1 cut(s)	926
XceI	RCATGY	2 cut(s)	367, 527
XcmI	CCANNNNNNNNNTGG	1 cut(s)	365
XmaJI	CCTAGG	1 cut(s)	715
XmiI	GTMKAC	2 cut(s)	751, 1452
XmnI	GAANNNNTTC	2 cut(s)	500, 918
XspI	CTAG	1 cut(s)	716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.