Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0006459

Physical Location & Seq

Genomic Coordinates Reverse (-)

62633481 .. 62639463

Gene Structure 5983 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG433400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGGAGAACGCAGCAAAAGAAGTTGTTGAGCTGAAATCTACCGTCCATGGTGGGGACAAAGACGTGTATGGTGAGGATACTGCCAGCGAGGACCAACCTGTCACGCCCTGGAGTGTGTCTGTTGCAAGTGGTTATAATTTGATACGAGATCCACACCACAACAAAGGTCTTGCCTTCTCTGAAAAGGAGAGAGATGCCCACTACATGCGTGGTCTTCTTCCCCCAGTTGTTATTTCTCAAGAACTTCAGGTGAAGAAAATGATAAACGCCATCCGCCAGTATCAAGTTCCCCTGCAGAAGTATATAGCCATGATGGATCTTCAGGAAAGAAACCAGAAGCTATTCTACAAGCTTCTTATCGAGCATGTTGAGGAATTACTCCCAGTTGTTTATACTCCAACCGTCGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCCAGCGTCCCCAGGGTCTATTCATAAGTTTGAAAGAAAAGGGAAAGATTCTTGAAGTGTTGCGGAACTGGCCAGAGAAGAATATTCAAGTCATTGTTGTCACTGATGGAGAGCGTATCCTAGGGCTGGGGGATCTTGGCTGCCATGGGATGGGTATACCTGTAGGCAAACTTTCTCTATATACGGCACTTGGAGGAGTTCGTCCATCTGCTTGCTTGCCTGTAACCATTGATGTGGGTACAAACAATGAGAAGCTGTTGGATGATGAGTTCTACATAGGGCTCAGGCAAAAGAGGGCAACTGGGCAGGAATATGCAGAACTCCTTCAAGAATTCATGACTGCTGTGAAGCAGAATTATGGAGAGAAAGTTCTTGTTCAGTTTGAGGACTTTGCAAACCACAATGCTTTTGATCTTCTTTCGAAGTATGGCGCAACTCATCTTGTTTTTAATGATGATATTCAGGGGACAGCATCTGTGGTCCTTGCAGGACTCTTGTCATCTTTGAAGTTAGTAGGGGGATCCTTAGCTGATCACACATTTTTATTCCTTGGTGCTGGAGAGGCTGGCACTGGCATAGCGGAGCTCATAGCCCTTGAAATATCTAAGCAGACAAATGCCCCACTGGAAGAAACTCGCAAGAAGGTTTGGCTCGTGGACTCGAAGGGATTGATTGTCAGTTCCCGGTTGGAATCACTCCAACATTTTAAGAAGCCTTGGGCTCATGAACATGAACCAGTCAAGACACTTGCAGAAGCTGTTAATGCAATCAAGCCAACGGTGTTGATTGGAACGTCAGGAGTAGGAAGAACATTTACTAAAGAAGTGGTTGAGGCTATGGCCACCAACAATGAGAGGCCCATTATTCTTGCTCTCTCCAACCCAACATCACAATCTGAGTGTACTGCTGAAGAAGCATATACATGGACACAGGGTCGTGCCATTTACTGTAGTGGAAGCCCATTCCCACCAGTTGAATACGAGGGAAAGGTTTACACTCCTGGCCAGTCGAATAATGCCTACATCTTTCCTGGATTTGGTCTTGGTTTATTGATGTCTGGTACCATTCGTGTTCATGATGACATGCTTCTGGCAGCCTCGGAAGCTTTGGCCTCACAGGTATCCCAGGAAGATTTTGACAAGGGACTTATATACCCTCCATTTACAAACATCAGAGAGATTTCAGCACATATTGCTGCTAAAGTAGCTGCTAAATCATATGAACTCGGTTTGGCTACACGCCTCCCCCAGCCAAAGGATCTGTTCCAGTATGCTGAAAGCTGTATGTACAGCCCAAACTACCGAAGTTTCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006090 GO:0006108 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016053 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

583

Amino Acids

64.36

Weight (kDa)

5.71

Isoelectric Point (pI)

40.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	108 - 288	6.2e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	299 - 550	3e-97	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79750
fragaria_vesca	FvH4_6g32330 FvH4_6g32330 FvH4_6g32330
malus_domestica	MD09G1203300.v1.1 MD17G1184200.v1.1
prunus_persica	Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1
pyrus_communis	pycom09g12010 pycom17g19390
rosa_chinensis	RchiOBHm_Chr2g0140441
rosa_laevigata	RLG00000019867
rosa_multiflora	Rmu_sc0003516.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436750 Rroxscaffold_2G00104830
rosa_rugosa	Rorug02G0362900 Rorug02G0363000 Rorug02G0363100 Rorug02G0363200 Rorug02G0363300
rosa_samantha	Rh2AG413500 Rh2BG423700 Rh2CG399700 Rh2DG433400
rosa_wichuraiana	Rw2G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	135
AasI	GACNNNNNNGTC	1 cut(s)	98
Acc65I	GGTACC	1 cut(s)	1496
AccB1I	GGYRCC	1 cut(s)	1496
AccB7I	CCANNNNNTGG	1 cut(s)	586
AccI	GTMKAC	1 cut(s)	592
AciI	CCGC	3 cut(s)	274, 499, 1016
AclWI	GGATC	6 cut(s)	143, 324, 576, 951, 964, 1701
AcoI	YGGCCR	3 cut(s)	506, 1275, 1438
AcsI	RAATTY	1 cut(s)	767
AcuI	CTGAAG	3 cut(s)	230, 305, 1365
AfaI	GTAC	4 cut(s)	676, 1339, 1498, 1724
AfiI	CCNNNNNNNGG	6 cut(s)	52, 404, 562, 586, 1472, 1690
AflIII	ACRYGT	1 cut(s)	63
AgsI	TTSAA	7 cut(s)	469, 491, 524, 764, 943, 1034, 1412
AhdI	GACNNNNNGTC	1 cut(s)	1368
AjiI	CACGTC	1 cut(s)	64
AjnI	CCWGG	5 cut(s)	107, 447, 1435, 1465, 1560
AloI	GAACNNNNNNTCC	4 cut(s)	689, 721, 789, 821
Alw21I	GWGCWC	1 cut(s)	1023
AlwI	GGATC	6 cut(s)	143, 324, 576, 951, 964, 1701
AlwNI	CAGNNNCTG	2 cut(s)	911, 1193
AoxI	GGCC	5 cut(s)	506, 1275, 1292, 1438, 1545
ApeKI	GCWGC	5 cut(s)	11, 576, 1529, 1631, 1643
ApoI	RAATTY	1 cut(s)	767
Asp700I	GAANNNNTTC	2 cut(s)	341, 759
Asp718I	GGTACC	1 cut(s)	1496
AspA2I	CCTAGG	1 cut(s)	556
AspLEI	GCGC	1 cut(s)	869
AspS9I	GGNCC	3 cut(s)	91, 916, 1293
AsuC2I	CCSGG	1 cut(s)	1120
AsuHPI	GGTGA	3 cut(s)	83, 262, 419
AsuII	TTCGAA	1 cut(s)	857
AvaII	GGWCC	2 cut(s)	91, 916
AvrII	CCTAGG	1 cut(s)	556
BalI	TGGCCA	3 cut(s)	508, 1277, 1440
BamHI	GGATCC	1 cut(s)	956
BanI	GGYRCC	1 cut(s)	1496
BanII	GRGCYC	3 cut(s)	720, 1023, 1159
BarI	GAAGNNNNNNTAC	4 cut(s)	329, 361, 1234, 1266
BauI	CACGAG	1 cut(s)	1088
BbsI	GAAGAC	1 cut(s)	206
Bbv12I	GWGCWC	1 cut(s)	1023
BbvI	GCAGC	5 cut(s)	23, 563, 1541, 1618, 1630
BccI	CCATC	5 cut(s)	278, 307, 536, 580, 649
BceAI	ACGGC	1 cut(s)	636
BciT130I	CCWGG	5 cut(s)	109, 449, 1437, 1467, 1562
BciVI	GTATCC	3 cut(s)	70, 563, 1567
BclI	TGATCA	1 cut(s)	967
BcnI	CCSGG	1 cut(s)	1120
BfaI	CTAG	1 cut(s)	557
BfmI	CTRYAG	3 cut(s)	293, 597, 1384
BfuI	GTATCC	3 cut(s)	70, 563, 1567
BisI	GCNGC	5 cut(s)	12, 577, 1530, 1632, 1644
BlnI	CCTAGG	1 cut(s)	556
BlsI	GCNGC	5 cut(s)	13, 578, 1531, 1633, 1645
Bme1390I	CCNGG	6 cut(s)	109, 449, 1120, 1437, 1467, 1562
Bme18I	GGWCC	2 cut(s)	91, 916
BmeRI	GACNNNNNGTC	1 cut(s)	1368
BmgBI	CACGTC	1 cut(s)	64
BmgT120I	GGNCC	3 cut(s)	91, 916, 1293
BmiI	GGNNCC	2 cut(s)	958, 1498
BmrFI	CCNGG	6 cut(s)	109, 449, 1120, 1437, 1467, 1562
BmrI	ACTGGG	3 cut(s)	218, 377, 747
BmsI	GCATC	3 cut(s)	184, 441, 917
BmuI	ACTGGG	3 cut(s)	218, 377, 747
BoxI	GACNNNNGTC	1 cut(s)	931
BpiI	GAAGAC	1 cut(s)	206
BpmI	CTGGAG	2 cut(s)	130, 1014
Bpu10I	CCTNAGC	2 cut(s)	719, 961
Bpu14I	TTCGAA	1 cut(s)	857
BpuEI	CTTGAG	1 cut(s)	222
BpuMI	CCSGG	1 cut(s)	1120
BsaWI	WCCGGW	1 cut(s)	1746
BsaXI	ACNNNNNCTCC	2 cut(s)	789, 819
Bsc4I	CCNNNNNNNGG	6 cut(s)	52, 404, 562, 586, 1472, 1690
BseBI	CCWGG	5 cut(s)	109, 449, 1437, 1467, 1562
BseGI	GGATG	3 cut(s)	270, 591, 703
BseLI	CCNNNNNNNGG	6 cut(s)	52, 404, 562, 586, 1472, 1690
BseMII	CTCAG	2 cut(s)	733, 1323
BseRI	GAGGAG	1 cut(s)	645
BseXI	GCAGC	5 cut(s)	23, 563, 1541, 1618, 1630
BseYI	CCCAGC	2 cut(s)	562, 1683
BshFI	GGCC	5 cut(s)	508, 1277, 1294, 1440, 1547
BshNI	GGYRCC	1 cut(s)	1496
BsiHKAI	GWGCWC	1 cut(s)	1023
BsiSI	CCGG	2 cut(s)	1120, 1747
BslFI	GGGAC	4 cut(s)	68, 429, 916, 1593
BslI	CCNNNNNNNGG	6 cut(s)	52, 404, 562, 586, 1472, 1690
BsmFI	GGGAC	4 cut(s)	68, 429, 916, 1593
BsnI	GGCC	5 cut(s)	508, 1277, 1294, 1440, 1547
Bsp119I	TTCGAA	1 cut(s)	857
Bsp1286I	GDGCHC	3 cut(s)	720, 1023, 1159
Bsp1407I	TGTACA	1 cut(s)	1722
Bsp143I	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
Bsp19I	CCATGG	2 cut(s)	46, 580
BspACI	CCGC	3 cut(s)	274, 499, 1016
BspANI	GGCC	5 cut(s)	508, 1277, 1294, 1440, 1547
BspCNI	CTCAG	2 cut(s)	732, 1324
BspHI	TCATGA	3 cut(s)	771, 1159, 1510
BspLI	GGNNCC	2 cut(s)	958, 1498
BspMAI	CTGCAG	1 cut(s)	297
BspPI	GGATC	6 cut(s)	143, 324, 576, 951, 964, 1701
BspT104I	TTCGAA	1 cut(s)	857
BspT107I	GGYRCC	1 cut(s)	1496
BsrGI	TGTACA	1 cut(s)	1722
BssMI	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
BssNAI	GTATAC	1 cut(s)	593
BssSI	CACGAG	1 cut(s)	1088
BssT1I	CCWWGG	5 cut(s)	46, 556, 580, 985, 1151
Bst1107I	GTATAC	1 cut(s)	593
Bst2BI	CACGAG	1 cut(s)	1088
Bst2UI	CCWGG	5 cut(s)	109, 449, 1437, 1467, 1562
Bst4CI	ACNGT	4 cut(s)	43, 403, 1216, 1385
BstAPI	GCANNNNNTGC	1 cut(s)	1628
BstAUI	TGTACA	1 cut(s)	1722
BstBI	TTCGAA	1 cut(s)	857
BstC8I	GCNNGC	5 cut(s)	85, 415, 649, 653, 1003
BstDEI	CTNAG	4 cut(s)	719, 961, 1041, 1332
BstDSI	CCRYGG	2 cut(s)	46, 580
BstF5I	GGATG	3 cut(s)	270, 591, 703
BstHHI	GCGC	1 cut(s)	869
BstKTI	GATC	7 cut(s)	151, 319, 571, 850, 959, 970, 1696
BstMBI	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
BstMWI	GCNNNNNNNGC	7 cut(s)	505, 998, 1199, 1349, 1538, 1628, 1640
BstNI	CCWGG	5 cut(s)	109, 449, 1437, 1467, 1562
BstNSI	RCATGY	3 cut(s)	208, 368, 1522
BstPAI	GACNNNNGTC	1 cut(s)	931
BstSCI	CCNGG	6 cut(s)	107, 447, 1118, 1435, 1465, 1560
BstSFI	CTRYAG	3 cut(s)	293, 597, 1384
BstV1I	GCAGC	5 cut(s)	23, 563, 1541, 1618, 1630
BstV2I	GAAGAC	1 cut(s)	206
BstX2I	RGATCY	5 cut(s)	148, 316, 568, 956, 1693
BstXI	CCANNNNNNTGG	2 cut(s)	425, 670
BstYI	RGATCY	5 cut(s)	148, 316, 568, 956, 1693
BstZ17I	GTATAC	1 cut(s)	593
BsuI	GTATCC	3 cut(s)	70, 563, 1567
BsuRI	GGCC	5 cut(s)	508, 1277, 1294, 1440, 1547
BtgI	CCRYGG	2 cut(s)	46, 580
BtrI	CACGTC	1 cut(s)	64
BtsCI	GGATG	3 cut(s)	270, 591, 703
BtsIMutI	CAGTG	3 cut(s)	537, 1005, 1058
Cac8I	GCNNGC	5 cut(s)	85, 415, 649, 653, 1003
CaiI	CAGNNNCTG	2 cut(s)	911, 1193
CciI	TCATGA	3 cut(s)	771, 1159, 1510
CfoI	GCGC	1 cut(s)	869
Cfr13I	GGNCC	3 cut(s)	91, 916, 1293
CseI	GACGC	1 cut(s)	431
Csp6I	GTAC	4 cut(s)	675, 1338, 1497, 1723
CviQI	GTAC	4 cut(s)	675, 1338, 1497, 1723
DdeI	CTNAG	4 cut(s)	719, 961, 1041, 1332
DpnI	GATC	7 cut(s)	150, 318, 570, 849, 958, 969, 1695
DpnII	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
DrdI	GACNNNNNNGTC	1 cut(s)	98
DriI	GACNNNNNGTC	1 cut(s)	1368
DseDI	GACNNNNNNGTC	1 cut(s)	98
EaeI	YGGCCR	3 cut(s)	506, 1275, 1438
Eam1105I	GACNNNNNGTC	1 cut(s)	1368
EciI	GGCGGA	1 cut(s)	263
Ecl136II	GAGCTC	1 cut(s)	1021
Eco130I	CCWWGG	5 cut(s)	46, 556, 580, 985, 1151
Eco24I	GRGCYC	3 cut(s)	720, 1023, 1159
Eco47I	GGWCC	2 cut(s)	91, 916
Eco53kI	GAGCTC	1 cut(s)	1021
Eco57I	CTGAAG	3 cut(s)	230, 305, 1365
EcoICRI	GAGCTC	1 cut(s)	1021
EcoRI	GAATTC	1 cut(s)	767
EcoRII	CCWGG	5 cut(s)	107, 447, 1435, 1465, 1560
EcoT14I	CCWWGG	5 cut(s)	46, 556, 580, 985, 1151
EcoT38I	GRGCYC	3 cut(s)	720, 1023, 1159
ErhI	CCWWGG	5 cut(s)	46, 556, 580, 985, 1151
FaqI	GGGAC	4 cut(s)	68, 429, 916, 1593
FauNDI	CATATG	1 cut(s)	1654
FbaI	TGATCA	1 cut(s)	967
FblI	GTMKAC	1 cut(s)	592
Fnu4HI	GCNGC	5 cut(s)	12, 577, 1530, 1632, 1644
FokI	GGATG	3 cut(s)	257, 598, 710
FriOI	GRGCYC	3 cut(s)	720, 1023, 1159
Fsp4HI	GCNGC	5 cut(s)	12, 577, 1530, 1632, 1644
FspBI	CTAG	1 cut(s)	557
GlaI	GCGC	1 cut(s)	868
GluI	GCNGC	5 cut(s)	12, 577, 1530, 1632, 1644
GsaI	CCCAGC	2 cut(s)	566, 1687
GsuI	CTGGAG	2 cut(s)	130, 1014
HaeIII	GGCC	5 cut(s)	508, 1277, 1294, 1440, 1547
HapII	CCGG	2 cut(s)	1120, 1747
HgaI	GACGC	1 cut(s)	431
HhaI	GCGC	1 cut(s)	869
Hin6I	GCGC	1 cut(s)	867
HinP1I	GCGC	1 cut(s)	867
HindIII	AAGCTT	3 cut(s)	350, 411, 1539
HinfI	GANTC	4 cut(s)	484, 927, 1094, 1127
HpaII	CCGG	2 cut(s)	1120, 1747
HphI	GGTGA	3 cut(s)	83, 262, 419
Hpy166II	GTNNAC	4 cut(s)	593, 1093, 1338, 1429
Hpy188I	TCNGA	4 cut(s)	181, 1333, 1537, 1610
Hpy188III	TCNNGA	9 cut(s)	239, 323, 488, 764, 772, 1160, 1177, 1233, 1511
Hpy8I	GTNNAC	4 cut(s)	593, 1093, 1338, 1429
Hpy99I	CGWCG	1 cut(s)	407
HpyAV	CCTTC	4 cut(s)	184, 770, 1072, 1093
HpyCH4III	ACNGT	4 cut(s)	43, 403, 1216, 1385
HpyCH4IV	ACGT	2 cut(s)	63, 1229
HpyCH4V	TGCA	7 cut(s)	125, 295, 752, 830, 923, 1187, 1202
HpyF10VI	GCNNNNNNNGC	7 cut(s)	505, 998, 1199, 1349, 1538, 1628, 1640
HpyF3I	CTNAG	4 cut(s)	719, 961, 1041, 1332
HpySE526I	ACGT	2 cut(s)	63, 1229
HspAI	GCGC	1 cut(s)	867
KpnI	GGTACC	1 cut(s)	1500
Ksp22I	TGATCA	1 cut(s)	967
Kzo9I	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
LmnI	GCTCC	1 cut(s)	1018
Lsp1109I	GCAGC	5 cut(s)	23, 563, 1541, 1618, 1630
LweI	GCATC	3 cut(s)	184, 441, 917
MaeI	CTAG	1 cut(s)	557
MaeII	ACGT	2 cut(s)	63, 1229
MaeIII	GTNAC	3 cut(s)	100, 535, 658
MalI	GATC	7 cut(s)	150, 318, 570, 849, 958, 969, 1695
MboI	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
MflI	RGATCY	5 cut(s)	148, 316, 568, 956, 1693
MhlI	GDGCHC	3 cut(s)	720, 1023, 1159
MlsI	TGGCCA	3 cut(s)	508, 1277, 1440
MluCI	AATT	4 cut(s)	136, 374, 767, 790
MluNI	TGGCCA	3 cut(s)	508, 1277, 1440
MlyI	GAGTC	2 cut(s)	921, 1088
MmeI	TCCRAC	5 cut(s)	422, 675, 1104, 1159, 1338
Mox20I	TGGCCA	3 cut(s)	508, 1277, 1440
MroXI	GAANNNNTTC	2 cut(s)	341, 759
MscI	TGGCCA	3 cut(s)	508, 1277, 1440
MseI	TTAA	3 cut(s)	885, 1143, 1197
MslI	CAYNNNNRTG	3 cut(s)	668, 1164, 1357
Msp20I	TGGCCA	3 cut(s)	508, 1277, 1440
MspI	CCGG	2 cut(s)	1120, 1747
MspR9I	CCNGG	6 cut(s)	109, 449, 1120, 1437, 1467, 1562
MvaI	CCWGG	5 cut(s)	109, 449, 1437, 1467, 1562
MwoI	GCNNNNNNNGC	7 cut(s)	505, 998, 1199, 1349, 1538, 1628, 1640
NciI	CCSGG	1 cut(s)	1120
NcoI	CCATGG	2 cut(s)	46, 580
NdeI	CATATG	1 cut(s)	1654
NdeII	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
NlaIV	GGNNCC	2 cut(s)	958, 1498
NmuCI	GTSAC	2 cut(s)	100, 535
NspI	RCATGY	3 cut(s)	208, 368, 1522
NspV	TTCGAA	1 cut(s)	857
PagI	TCATGA	3 cut(s)	771, 1159, 1510
PasI	CCCWGGG	1 cut(s)	448
PdmI	GAANNNNTTC	2 cut(s)	341, 759
PfeI	GAWTC	2 cut(s)	484, 1127
PflMI	CCANNNNNTGG	1 cut(s)	586
PfoI	TCCNGGA	1 cut(s)	1465
PkrI	GCNGC	5 cut(s)	13, 578, 1531, 1633, 1645
PleI	GAGTC	2 cut(s)	921, 1088
PpsI	GAGTC	2 cut(s)	921, 1088
PshAI	GACNNNNGTC	1 cut(s)	931
PsiI	TTATAA	1 cut(s)	135
Psp124BI	GAGCTC	1 cut(s)	1023
Psp6I	CCWGG	5 cut(s)	107, 447, 1435, 1465, 1560
PspFI	CCCAGC	2 cut(s)	562, 1683
PspGI	CCWGG	5 cut(s)	107, 447, 1435, 1465, 1560
PspN4I	GGNNCC	2 cut(s)	958, 1498
PspPI	GGNCC	3 cut(s)	91, 916, 1293
PstI	CTGCAG	1 cut(s)	297
PstNI	CAGNNNCTG	2 cut(s)	911, 1193
PsuI	RGATCY	5 cut(s)	148, 316, 568, 956, 1693
RsaI	GTAC	4 cut(s)	676, 1339, 1498, 1724
RsaNI	GTAC	4 cut(s)	675, 1338, 1497, 1723
RseI	CAYNNNNRTG	3 cut(s)	668, 1164, 1357
SacI	GAGCTC	1 cut(s)	1023
SaqAI	TTAA	3 cut(s)	885, 1143, 1197
SatI	GCNGC	5 cut(s)	12, 577, 1530, 1632, 1644
Sau3AI	GATC	7 cut(s)	148, 316, 568, 847, 956, 967, 1693
Sau96I	GGNCC	3 cut(s)	91, 916, 1293
SchI	GAGTC	2 cut(s)	921, 1088
ScrFI	CCNGG	6 cut(s)	109, 449, 1120, 1437, 1467, 1562
SduI	GDGCHC	3 cut(s)	720, 1023, 1159
SfaNI	GCATC	3 cut(s)	184, 441, 917
SfcI	CTRYAG	3 cut(s)	293, 597, 1384
SfuI	TTCGAA	1 cut(s)	857
SinI	GGWCC	2 cut(s)	91, 916
SmiMI	CAYNNNNRTG	3 cut(s)	668, 1164, 1357
SmlI	CTYRAG	1 cut(s)	237
SmoI	CTYRAG	1 cut(s)	237
Sse9I	AATT	4 cut(s)	136, 374, 767, 790
SsiI	CCGC	3 cut(s)	274, 499, 1016
SspI	AATATT	1 cut(s)	520
SspMI	CTAG	1 cut(s)	557
SstI	GAGCTC	1 cut(s)	1023
StyD4I	CCNGG	6 cut(s)	107, 447, 1118, 1435, 1465, 1560
StyI	CCWWGG	5 cut(s)	46, 556, 580, 985, 1151
TaaI	ACNGT	4 cut(s)	43, 403, 1216, 1385
TaiI	ACGT	2 cut(s)	66, 1232
TaqI	TCGA	4 cut(s)	360, 857, 1097, 1445
TasI	AATT	4 cut(s)	136, 374, 767, 790
TatI	WGTACW	2 cut(s)	1337, 1722
TfiI	GAWTC	2 cut(s)	484, 1127
Tru1I	TTAA	3 cut(s)	885, 1143, 1197
Tru9I	TTAA	3 cut(s)	885, 1143, 1197
TscAI	CASTG	3 cut(s)	544, 1012, 1065
TseFI	GTSAC	2 cut(s)	100, 535
TseI	GCWGC	5 cut(s)	11, 576, 1529, 1631, 1643
Tsp45I	GTSAC	2 cut(s)	100, 535
TspDTI	ATGAA	6 cut(s)	448, 760, 1176, 1182, 1499, 1671
TspRI	CASTG	3 cut(s)	544, 1012, 1065
Van91I	CCANNNNNTGG	1 cut(s)	586
VpaK11BI	GGWCC	2 cut(s)	91, 916
XapI	RAATTY	1 cut(s)	767
XceI	RCATGY	3 cut(s)	208, 368, 1522
XcmI	CCANNNNNNNNNTGG	1 cut(s)	206
XmaJI	CCTAGG	1 cut(s)	556
XmiI	GTMKAC	1 cut(s)	592
XmnI	GAANNNNTTC	2 cut(s)	341, 759
XspI	CTAG	1 cut(s)	557

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.