Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0006459

Physical Location & Seq

Genomic Coordinates Reverse (-)

21751240 .. 21757315

Gene Structure 6076 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1184200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1926 bp

ATGATGTCCTTGAATAGAAGCTGTTTTCTGGGGAAACCGGGCATTGCTGGGTCTTCAAGCCCGTTCTCTCAGAGCCAGAAGAGGCGGTCAGCGTCGCTAAAAGTGGTGGCTGTGGTCCCAAAAGTCCGGACAAGCGACCGAAACGGTAGCGTTGTGATGGAGAACCCTTTGCAGGAGGTGAAGGCCGAATCCGAGGTGGTTGAGCTGAAATCTACCGTCCATGGTGGGGTACAAGACGTGTACGGTGAGGATACTGCCACCGAGGACCAACTCGTCACGCCCTGGAGTGTCTCGGTTGCTAGTGGATATACATTGATACGATCTCCACACCACAACAAAGGTCTTGCTTTCACAGAGAAAGAGAGAGATGCCCACTACTTGCGTGGTCTTCTTCCCCCAGTTGTTATTTCTCAAGAACTTCAGGTTAAGAAAATGATCAACAGTATCCGTCAGTATCAAGTTCCACTGCAGAAGTATATCGCTATGATGGATCTTCAGGGGAGAAATGAAAAGCTATTCTACAAGCTTCTTATTGAACATGTTGAGGAATTACTTCCGGTTGTCTATACTCCTACTGTCGGTGAAGCTTGCCAGAAATATGGAAGCATCTTTACGCAACCACAGGGTCTTTTCATAAGTTTGAAAGAAAAGGGCAAGATTCTTGAAGTATTGAGGAACTGGCCTGAGAAGAATATTCAAGTCATTGTTGTCACTGATGGAGAGCGGATCCTAGGGCTTGGGGATCTTGGCTGTCATGGAATGGGTATTCCTGTGGGTAAACTTGCTCTATACACGGCACTTGGAGGAGTTCGTCCTTCTGCTTGCTTGCCTGTAACCATTGATGTTGGTACAAACAATGAGAAGCTGTTGAATGACGAGTTCTACATAGGGCTGAGGCAAAAGAGGGCTACTGGGCAGGAATATGCCGAACTCCTACAAGAGTTCATGACTGCAGTCAAGCAGAATTACGGGGAGAAAGTTCTCGTTCAGTTTGAAGACTTTGCAAACCACAATGCTTTTGAACTACTTGCAAAGTATGCCTCAACTCATCTTGTTTTTAATGACGACATTCAGGGGACAGCATCCGTGGTTCTGGCTGGGCTTCTTGCGGCTTTGAAGTTAGTAAAGGGATCTTTATCTGAACACAGATTTTTATTCCTTGGTGCTGGAGAGGCCGGCACTGGCATAGCAGAACTCATTGCCCTTGAAGTTTCCAAGCAGACAAATACCCCAGTGGAAGAGGCCCGCAAGAACATTTGGCTTGTGGACTCAAAGGGATTGATTGTCAGTTCTCGGCTGGATTCACTCCAACACTTTAAAAAGCCCTGGGCTCATGAACATGAACCAGTCAGGGAACTTGTAAATGCTGTTAAGTCAATCAAGCCAACGGTGTTGATTGGAACGTCAGGAGTAGGAAGAACATTCACTAAAGAAGTGGTTGAGGCTATGGCCTCCCTGAATGAGAGACCTATTATTCTTGCTCTTTCCAACCCAACATCACAATCTGAATGTACTGCAGAAGAAGCATATACATGGACTGAGGGTCGTGCCATGTATTGTAGTGGAAGCCCATTCCCACCGGTTGAATATAATGGGAAGGTTTACTATCCTGGCCAGTCAAACAATGCATACATCTTCCCTGGATTCGGTCTGGGTTTAATAATGTCTGGTACTATCCGTGTTCACGACGACATGCTTCTGGCAGCCTCGGAAGCGTTGGCTTCAATGGTGACCCAGGAAGATTTTGACAAGGGACTCATATACCCTCCATTCACAAACATCAGAAAGATTTCGGCGCATATTGCTGCTAAAGTAGCTGCGAAATCATATGAACTTGGTTTGGCTACTCGCCTCCCCGAGCCAAGAGATCTGGAGAGGTATGCTGAAAGCTGTATGTACAGCCCTTCCTATCGAAGTTTCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006090 GO:0006108 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016053 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

642

Amino Acids

70.69

Weight (kDa)

6.73

Isoelectric Point (pI)

46.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	166 - 346	1.6e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	357 - 608	3.5e-98	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79750
fragaria_vesca	FvH4_6g32330 FvH4_6g32330 FvH4_6g32330
malus_domestica	MD09G1203300.v1.1 MD17G1184200.v1.1
prunus_persica	Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1
pyrus_communis	pycom09g12010 pycom17g19390
rosa_chinensis	RchiOBHm_Chr2g0140441
rosa_laevigata	RLG00000019867
rosa_multiflora	Rmu_sc0003516.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436750 Rroxscaffold_2G00104830
rosa_rugosa	Rorug02G0362900 Rorug02G0363000 Rorug02G0363100 Rorug02G0363200 Rorug02G0363300
rosa_samantha	Rh2AG413500 Rh2BG423700 Rh2CG399700 Rh2DG433400
rosa_wichuraiana	Rw2G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	272
AccBSI	CCGCTC	1 cut(s)	724
AccIII	TCCGGA	1 cut(s)	126
AciI	CCGC	4 cut(s)	85, 724, 1109, 1246
AclWI	GGATC	5 cut(s)	498, 721, 734, 750, 1138
AcoI	YGGCCR	1 cut(s)	1612
AcuI	CTGAAG	2 cut(s)	404, 479
AfaI	GTAC	6 cut(s)	231, 242, 850, 1513, 1672, 1898
AfiI	CCNNNNNNNGG	4 cut(s)	172, 226, 578, 1646
AflIII	ACRYGT	2 cut(s)	237, 538
AgeI	ACCGGT	1 cut(s)	1579
AhdI	GACNNNNNGTC	1 cut(s)	1542
AjiI	CACGTC	1 cut(s)	238
AjnI	CCWGG	5 cut(s)	281, 1325, 1609, 1639, 1734
AluBI	AGCT	8 cut(s)	21, 205, 514, 526, 587, 865, 1817, 1890
AluI	AGCT	8 cut(s)	21, 205, 514, 526, 587, 865, 1817, 1890
Alw26I	GTCTC	2 cut(s)	295, 1459
AlwI	GGATC	5 cut(s)	498, 721, 734, 750, 1138
Ama87I	CYCGRG	1 cut(s)	1856
Aor13HI	TCCGGA	1 cut(s)	126
AoxI	GGCC	6 cut(s)	183, 680, 1173, 1242, 1449, 1612
ApeKI	GCWGC	3 cut(s)	1703, 1805, 1817
AsiGI	ACCGGT	1 cut(s)	1579
Asp700I	GAANNNNTTC	2 cut(s)	552, 1789
AspA2I	CCTAGG	1 cut(s)	730
AspLEI	GCGC	1 cut(s)	1798
AspS9I	GGNCC	3 cut(s)	115, 265, 1243
AsuC2I	CCSGG	1 cut(s)	39
AsuHPI	GGTGA	4 cut(s)	190, 257, 593, 1741
AvaI	CYCGRG	1 cut(s)	1856
AvaII	GGWCC	2 cut(s)	115, 265
AvrII	CCTAGG	1 cut(s)	730
BalI	TGGCCA	1 cut(s)	1614
BamHI	GGATCC	1 cut(s)	726
BanII	GRGCYC	1 cut(s)	1333
BarI	GAAGNNNNNNTAC	2 cut(s)	595, 627
BbsI	GAAGAC	3 cut(s)	45, 380, 1002
BbvCI	CCTCAGC	1 cut(s)	893
BbvI	GCAGC	3 cut(s)	1715, 1792, 1804
BccI	CCATC	3 cut(s)	151, 481, 710
BceAI	ACGGC	1 cut(s)	810
BciT130I	CCWGG	5 cut(s)	283, 1327, 1611, 1641, 1736
BciVI	GTATCC	2 cut(s)	244, 455
BclI	TGATCA	1 cut(s)	435
BcnI	CCSGG	1 cut(s)	39
BcoDI	GTCTC	2 cut(s)	295, 1459
BfaI	CTAG	2 cut(s)	300, 731
BfmI	CTRYAG	3 cut(s)	467, 951, 1515
BfuI	GTATCC	2 cut(s)	244, 455
BglII	AGATCT	1 cut(s)	1867
BisI	GCNGC	4 cut(s)	1110, 1704, 1806, 1818
BlnI	CCTAGG	1 cut(s)	730
BlsI	GCNGC	4 cut(s)	1111, 1705, 1807, 1819
Bme1390I	CCNGG	6 cut(s)	39, 283, 1327, 1611, 1641, 1736
Bme18I	GGWCC	2 cut(s)	115, 265
BmeRI	GACNNNNNGTC	1 cut(s)	1542
BmeT110I	CYCGRG	1 cut(s)	1856
BmgBI	CACGTC	1 cut(s)	238
BmgT120I	GGNCC	3 cut(s)	115, 265, 1243
BmiI	GGNNCC	2 cut(s)	117, 728
BmrFI	CCNGG	6 cut(s)	39, 283, 1327, 1611, 1641, 1736
BmrI	ACTGGG	3 cut(s)	392, 921, 1226
BmsI	GCATC	3 cut(s)	358, 615, 1091
BmuI	ACTGGG	3 cut(s)	392, 921, 1226
BoxI	GACNNNNGTC	1 cut(s)	953
BpiI	GAAGAC	3 cut(s)	45, 380, 1002
BpmI	CTGGAG	3 cut(s)	304, 1188, 1892
Bpu10I	CCTNAGC	1 cut(s)	893
BpuEI	CTTGAG	1 cut(s)	396
BpuMI	CCSGG	1 cut(s)	39
BsaBI	GATNNNNATC	1 cut(s)	1135
BsaI	GGTCTC	1 cut(s)	1459
BsaWI	WCCGGW	4 cut(s)	126, 556, 1579, 1920
Bsc4I	CCNNNNNNNGG	4 cut(s)	172, 226, 578, 1646
Bse118I	RCCGGY	2 cut(s)	1175, 1579
Bse1I	ACTGG	7 cut(s)	398, 683, 916, 1186, 1232, 1346, 1615
Bse3DI	GCAATG	2 cut(s)	42, 1197
Bse8I	GATNNNNATC	1 cut(s)	1135
BseAI	TCCGGA	1 cut(s)	126
BseBI	CCWGG	5 cut(s)	283, 1327, 1611, 1641, 1736
BseGI	GGATG	1 cut(s)	1082
BseJI	GATNNNNATC	1 cut(s)	1135
BseLI	CCNNNNNNNGG	4 cut(s)	172, 226, 578, 1646
BseMI	GCAATG	2 cut(s)	42, 1197
BseMII	CTCAG	4 cut(s)	83, 675, 884, 1530
BseNI	ACTGG	7 cut(s)	398, 683, 916, 1186, 1232, 1346, 1615
BseRI	GAGGAG	1 cut(s)	819
BseXI	GCAGC	3 cut(s)	1715, 1792, 1804
BseYI	CCCAGC	2 cut(s)	47, 1097
Bsh1285I	CGRYCG	1 cut(s)	139
BshFI	GGCC	6 cut(s)	185, 682, 1175, 1244, 1451, 1614
BshTI	ACCGGT	1 cut(s)	1579
BsiEI	CGRYCG	1 cut(s)	139
BsiHKCI	CYCGRG	1 cut(s)	1856
BsiSI	CCGG	6 cut(s)	38, 127, 557, 1176, 1580, 1921
BslFI	GGGAC	3 cut(s)	101, 1090, 1767
BslI	CCNNNNNNNGG	4 cut(s)	172, 226, 578, 1646
BsmAI	GTCTC	2 cut(s)	295, 1459
BsmFI	GGGAC	3 cut(s)	101, 1090, 1767
BsnI	GGCC	6 cut(s)	185, 682, 1175, 1244, 1451, 1614
Bso31I	GGTCTC	1 cut(s)	1459
BsoBI	CYCGRG	1 cut(s)	1856
Bsp1286I	GDGCHC	1 cut(s)	1333
Bsp13I	TCCGGA	1 cut(s)	126
Bsp1407I	TGTACA	1 cut(s)	1896
Bsp143I	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
Bsp19I	CCATGG	1 cut(s)	220
BspACI	CCGC	4 cut(s)	85, 724, 1109, 1246
BspANI	GGCC	6 cut(s)	185, 682, 1175, 1244, 1451, 1614
BspCNI	CTCAG	4 cut(s)	82, 676, 885, 1531
BspEI	TCCGGA	1 cut(s)	126
BspHI	TCATGA	2 cut(s)	945, 1333
BspLI	GGNNCC	2 cut(s)	117, 728
BspMAI	CTGCAG	3 cut(s)	471, 955, 1519
BspPI	GGATC	5 cut(s)	498, 721, 734, 750, 1138
BspTNI	GGTCTC	1 cut(s)	1459
BsrBI	CCGCTC	1 cut(s)	724
BsrDI	GCAATG	2 cut(s)	42, 1197
BsrFI	RCCGGY	2 cut(s)	1175, 1579
BsrGI	TGTACA	1 cut(s)	1896
BsrI	ACTGG	7 cut(s)	398, 683, 916, 1186, 1232, 1346, 1615
BssAI	RCCGGY	2 cut(s)	1175, 1579
BssMI	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
BssT1I	CCWWGG	3 cut(s)	220, 730, 1159
Bst2UI	CCWGG	5 cut(s)	283, 1327, 1611, 1641, 1736
Bst4CI	ACNGT	6 cut(s)	146, 217, 245, 443, 577, 1390
Bst6I	CTCTTC	2 cut(s)	74, 1233
BstAPI	GCANNNNNTGC	1 cut(s)	1037
BstAUI	TGTACA	1 cut(s)	1896
BstC8I	GCNNGC	5 cut(s)	589, 823, 827, 1177, 1246
BstDEI	CTNAG	4 cut(s)	69, 684, 893, 1539
BstDSI	CCRYGG	2 cut(s)	220, 1086
BstEII	GGTNACC	1 cut(s)	1729
BstF5I	GGATG	1 cut(s)	1082
BstHHI	GCGC	1 cut(s)	1798
BstKTI	GATC	7 cut(s)	323, 438, 493, 729, 745, 1133, 1870
BstMAI	GTCTC	2 cut(s)	295, 1459
BstMBI	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
BstMCI	CGRYCG	1 cut(s)	139
BstMWI	GCNNNNNNNGC	6 cut(s)	1037, 1172, 1523, 1712, 1802, 1814
BstNI	CCWGG	5 cut(s)	283, 1327, 1611, 1641, 1736
BstNSI	RCATGY	2 cut(s)	542, 1696
BstPAI	GACNNNNGTC	1 cut(s)	953
BstPI	GGTNACC	1 cut(s)	1729
BstSCI	CCNGG	6 cut(s)	37, 281, 1325, 1609, 1639, 1734
BstSFI	CTRYAG	3 cut(s)	467, 951, 1515
BstV1I	GCAGC	3 cut(s)	1715, 1792, 1804
BstV2I	GAAGAC	3 cut(s)	45, 380, 1002
BstX2I	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
BstXI	CCANNNNNNTGG	1 cut(s)	599
BstYI	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
BsuI	GTATCC	2 cut(s)	244, 455
BsuRI	GGCC	6 cut(s)	185, 682, 1175, 1244, 1451, 1614
BtgI	CCRYGG	2 cut(s)	220, 1086
BtrI	CACGTC	1 cut(s)	238
BtsCI	GGATG	1 cut(s)	1082
BtsI	GCAGTG	1 cut(s)	464
BtsIMutI	CAGTG	4 cut(s)	464, 711, 1179, 1239
Cac8I	GCNNGC	5 cut(s)	589, 823, 827, 1177, 1246
CciI	TCATGA	2 cut(s)	945, 1333
CfoI	GCGC	1 cut(s)	1798
Cfr10I	RCCGGY	2 cut(s)	1175, 1579
Cfr13I	GGNCC	3 cut(s)	115, 265, 1243
CseI	GACGC	1 cut(s)	81
Csp6I	GTAC	6 cut(s)	230, 241, 849, 1512, 1671, 1897
CspAI	ACCGGT	1 cut(s)	1579
CviAII	CATG	9 cut(s)	221, 539, 755, 946, 1334, 1340, 1533, 1552, 1693
CviQI	GTAC	6 cut(s)	230, 241, 849, 1512, 1671, 1897
DdeI	CTNAG	4 cut(s)	69, 684, 893, 1539
DpnI	GATC	7 cut(s)	322, 437, 492, 728, 744, 1132, 1869
DpnII	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
DraI	TTTAAA	1 cut(s)	1318
DrdI	GACNNNNNNGTC	1 cut(s)	272
DriI	GACNNNNNGTC	1 cut(s)	1542
DseDI	GACNNNNNNGTC	1 cut(s)	272
EaeI	YGGCCR	1 cut(s)	1612
Eam1104I	CTCTTC	2 cut(s)	74, 1233
Eam1105I	GACNNNNNGTC	1 cut(s)	1542
EarI	CTCTTC	2 cut(s)	74, 1233
Eco130I	CCWWGG	3 cut(s)	220, 730, 1159
Eco24I	GRGCYC	1 cut(s)	1333
Eco31I	GGTCTC	1 cut(s)	1459
Eco47I	GGWCC	2 cut(s)	115, 265
Eco57I	CTGAAG	2 cut(s)	404, 479
Eco88I	CYCGRG	1 cut(s)	1856
Eco91I	GGTNACC	1 cut(s)	1729
EcoO65I	GGTNACC	1 cut(s)	1729
EcoRII	CCWGG	5 cut(s)	281, 1325, 1609, 1639, 1734
EcoT14I	CCWWGG	3 cut(s)	220, 730, 1159
EcoT22I	ATGCAT	1 cut(s)	1630
EcoT38I	GRGCYC	1 cut(s)	1333
ErhI	CCWWGG	3 cut(s)	220, 730, 1159
FaeI	CATG	9 cut(s)	224, 542, 758, 949, 1337, 1343, 1536, 1555, 1696
FaqI	GGGAC	3 cut(s)	101, 1090, 1767
FatI	CATG	9 cut(s)	220, 538, 754, 945, 1333, 1339, 1532, 1551, 1692
FauI	CCCGC	1 cut(s)	1253
FauNDI	CATATG	1 cut(s)	1828
FbaI	TGATCA	1 cut(s)	435
Fnu4HI	GCNGC	4 cut(s)	1110, 1704, 1806, 1818
FokI	GGATG	1 cut(s)	1069
FriOI	GRGCYC	1 cut(s)	1333
Fsp4HI	GCNGC	4 cut(s)	1110, 1704, 1806, 1818
FspBI	CTAG	2 cut(s)	300, 731
GlaI	GCGC	1 cut(s)	1797
GluI	GCNGC	4 cut(s)	1110, 1704, 1806, 1818
GsaI	CCCAGC	2 cut(s)	51, 1101
GsuI	CTGGAG	3 cut(s)	304, 1188, 1892
HaeIII	GGCC	6 cut(s)	185, 682, 1175, 1244, 1451, 1614
HapII	CCGG	6 cut(s)	38, 127, 557, 1176, 1580, 1921
HgaI	GACGC	1 cut(s)	81
HhaI	GCGC	1 cut(s)	1798
Hin1II	CATG	9 cut(s)	224, 542, 758, 949, 1337, 1343, 1536, 1555, 1696
Hin6I	GCGC	1 cut(s)	1796
HinP1I	GCGC	1 cut(s)	1796
HindIII	AAGCTT	2 cut(s)	524, 585
HinfI	GANTC	6 cut(s)	188, 658, 1268, 1301, 1644, 1755
HpaII	CCGG	6 cut(s)	38, 127, 557, 1176, 1580, 1921
HphI	GGTGA	4 cut(s)	190, 257, 593, 1741
Hpy166II	GTNNAC	5 cut(s)	241, 779, 1267, 1603, 1684
Hpy188I	TCNGA	6 cut(s)	72, 193, 1141, 1507, 1711, 1784
Hpy188III	TCNNGA	8 cut(s)	127, 413, 662, 946, 1334, 1407, 1685, 1871
Hpy8I	GTNNAC	5 cut(s)	241, 779, 1267, 1603, 1684
Hpy99I	CGWCG	2 cut(s)	97, 1691
HpyAV	CCTTC	4 cut(s)	175, 825, 1591, 1914
HpyCH4III	ACNGT	6 cut(s)	146, 217, 245, 443, 577, 1390
HpyCH4IV	ACGT	2 cut(s)	237, 1403
HpyCH4V	TGCA	7 cut(s)	172, 469, 953, 1004, 1031, 1517, 1628
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1037, 1172, 1523, 1712, 1802, 1814
HpyF3I	CTNAG	4 cut(s)	69, 684, 893, 1539
HpySE526I	ACGT	2 cut(s)	237, 1403
Hsp92II	CATG	9 cut(s)	224, 542, 758, 949, 1337, 1343, 1536, 1555, 1696
HspAI	GCGC	1 cut(s)	1796
Kpn2I	TCCGGA	1 cut(s)	126
KroI	GCCGGC	1 cut(s)	1175
KroNI	GCCGGC	1 cut(s)	1177
Ksp22I	TGATCA	1 cut(s)	435
Kzo9I	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
Lsp1109I	GCAGC	3 cut(s)	1715, 1792, 1804
LweI	GCATC	3 cut(s)	358, 615, 1091
MaeI	CTAG	2 cut(s)	300, 731
MaeII	ACGT	2 cut(s)	237, 1403
MaeIII	GTNAC	4 cut(s)	274, 709, 832, 1729
MalI	GATC	7 cut(s)	322, 437, 492, 728, 744, 1132, 1869
MbiI	CCGCTC	1 cut(s)	724
MboI	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
MflI	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
MhlI	GDGCHC	1 cut(s)	1333
MlsI	TGGCCA	1 cut(s)	1614
MluCI	AATT	2 cut(s)	548, 964
MluNI	TGGCCA	1 cut(s)	1614
MlyI	GAGTC	2 cut(s)	1262, 1749
MmeI	TCCRAC	2 cut(s)	1333, 1512
Mox20I	TGGCCA	1 cut(s)	1614
Mph1103I	ATGCAT	1 cut(s)	1630
MroI	TCCGGA	1 cut(s)	126
MroNI	GCCGGC	1 cut(s)	1175
MroXI	GAANNNNTTC	2 cut(s)	552, 1789
MscI	TGGCCA	1 cut(s)	1614
MseI	TTAA	5 cut(s)	426, 1059, 1317, 1371, 1658
MslI	CAYNNNNRTG	2 cut(s)	1338, 1531
Msp20I	TGGCCA	1 cut(s)	1614
MspI	CCGG	6 cut(s)	38, 127, 557, 1176, 1580, 1921
MspR9I	CCNGG	6 cut(s)	39, 283, 1327, 1611, 1641, 1736
MvaI	CCWGG	5 cut(s)	283, 1327, 1611, 1641, 1736
MwoI	GCNNNNNNNGC	6 cut(s)	1037, 1172, 1523, 1712, 1802, 1814
NaeI	GCCGGC	1 cut(s)	1177
NciI	CCSGG	1 cut(s)	39
NcoI	CCATGG	1 cut(s)	220
NdeI	CATATG	1 cut(s)	1828
NdeII	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
NgoMIV	GCCGGC	1 cut(s)	1175
NlaIII	CATG	9 cut(s)	224, 542, 758, 949, 1337, 1343, 1536, 1555, 1696
NlaIV	GGNNCC	2 cut(s)	117, 728
NmeAIII	GCCGAG	1 cut(s)	1273
NmuCI	GTSAC	3 cut(s)	274, 709, 1729
NsiI	ATGCAT	1 cut(s)	1630
NspI	RCATGY	2 cut(s)	542, 1696
PagI	TCATGA	2 cut(s)	945, 1333
PasI	CCCWGGG	1 cut(s)	1326
PciI	ACATGT	1 cut(s)	538
PdiI	GCCGGC	1 cut(s)	1177
PdmI	GAANNNNTTC	2 cut(s)	552, 1789
PfeI	GAWTC	4 cut(s)	188, 658, 1301, 1644
PinAI	ACCGGT	1 cut(s)	1579
PkrI	GCNGC	4 cut(s)	1111, 1705, 1807, 1819
PleI	GAGTC	2 cut(s)	1262, 1749
PpsI	GAGTC	2 cut(s)	1262, 1749
PscI	ACATGT	1 cut(s)	538
PshAI	GACNNNNGTC	1 cut(s)	953
Psp6I	CCWGG	5 cut(s)	281, 1325, 1609, 1639, 1734
PspEI	GGTNACC	1 cut(s)	1729
PspFI	CCCAGC	2 cut(s)	47, 1097
PspGI	CCWGG	5 cut(s)	281, 1325, 1609, 1639, 1734
PspN4I	GGNNCC	2 cut(s)	117, 728
PspPI	GGNCC	3 cut(s)	115, 265, 1243
PstI	CTGCAG	3 cut(s)	471, 955, 1519
PsuI	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
RsaI	GTAC	6 cut(s)	231, 242, 850, 1513, 1672, 1898
RsaNI	GTAC	6 cut(s)	230, 241, 849, 1512, 1671, 1897
RseI	CAYNNNNRTG	2 cut(s)	1338, 1531
SaqAI	TTAA	5 cut(s)	426, 1059, 1317, 1371, 1658
SatI	GCNGC	4 cut(s)	1110, 1704, 1806, 1818
Sau3AI	GATC	7 cut(s)	320, 435, 490, 726, 742, 1130, 1867
Sau96I	GGNCC	3 cut(s)	115, 265, 1243
SchI	GAGTC	2 cut(s)	1262, 1749
ScrFI	CCNGG	6 cut(s)	39, 283, 1327, 1611, 1641, 1736
SduI	GDGCHC	1 cut(s)	1333
SfaNI	GCATC	3 cut(s)	358, 615, 1091
SfcI	CTRYAG	3 cut(s)	467, 951, 1515
SinI	GGWCC	2 cut(s)	115, 265
SmiMI	CAYNNNNRTG	2 cut(s)	1338, 1531
SmlI	CTYRAG	1 cut(s)	411
SmoI	CTYRAG	1 cut(s)	411
Sse9I	AATT	2 cut(s)	548, 964
SsiI	CCGC	4 cut(s)	85, 724, 1109, 1246
SspI	AATATT	1 cut(s)	694
SspMI	CTAG	2 cut(s)	300, 731
StyD4I	CCNGG	6 cut(s)	37, 281, 1325, 1609, 1639, 1734
StyI	CCWWGG	3 cut(s)	220, 730, 1159
TaaI	ACNGT	6 cut(s)	146, 217, 245, 443, 577, 1390
TaiI	ACGT	2 cut(s)	240, 1406
TaqI	TCGA	1 cut(s)	1912
TaqII	GACCGA	2 cut(s)	153, 1637
TasI	AATT	2 cut(s)	548, 964
TatI	WGTACW	2 cut(s)	1511, 1896
TauI	GCSGC	1 cut(s)	1112
TfiI	GAWTC	4 cut(s)	188, 658, 1301, 1644
Tru1I	TTAA	5 cut(s)	426, 1059, 1317, 1371, 1658
Tru9I	TTAA	5 cut(s)	426, 1059, 1317, 1371, 1658
TscAI	CASTG	4 cut(s)	471, 718, 1186, 1239
TseFI	GTSAC	3 cut(s)	274, 709, 1729
TseI	GCWGC	3 cut(s)	1703, 1805, 1817
Tsp45I	GTSAC	3 cut(s)	274, 709, 1729
TspDTI	ATGAA	6 cut(s)	522, 622, 934, 1350, 1356, 1845
TspGWI	ACGGA	3 cut(s)	437, 1075, 1667
TspRI	CASTG	4 cut(s)	471, 718, 1186, 1239
VpaK11BI	GGWCC	2 cut(s)	115, 265
XceI	RCATGY	2 cut(s)	542, 1696
XcmI	CCANNNNNNNNNTGG	1 cut(s)	380
XmaJI	CCTAGG	1 cut(s)	730
XmnI	GAANNNNTTC	2 cut(s)	552, 1789
XspI	CTAG	2 cut(s)	300, 731
Zsp2I	ATGCAT	1 cut(s)	1630

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.