Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0006459

Physical Location & Seq

Genomic Coordinates Forward (+)

28158812 .. 28165018

Gene Structure 6207 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00104830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGATCTCGCTGAATAGAAGCTGCTTCCTGAGCAACGGGGGCATTTCTGGGAATTGTAGCCCGTTCTCTCAGGGGCAGAAGAGGCGGTCGGCTTCGCTGAAAGTGGTGGCTGTGGGGACGAACGCCGCCAGGAAAGCTGAACGAAACGGCAGCATAGTCATGGAGAACGCAGCAAAAGAAGTTGTTGAGCTGAAATCTACCGTCCATGGTGGGGACAAAGACGTGTATGGTGAGGATACTGCCAGCGAGGACCAACCTGTCACGCCCTGGAGTGTGTCTGTTGCAAGTGGTTATAATTTGATACGAGATCCACACCACAACAAAGGTCTTGCCTTCTCTGAAAAGGAGAGAGATGCCCACTACATGCGTGGTCTTCTTCCCCCAGTTGTTATTTCTCAAGAACTTCAGGTGAAGAAAATGATAAACGCCATCCGCCAGTATCAAGTTCCCCTGCAGAAGTATATAGCCATGATGGATCTTCAGGAAAGAAACCAGAAGCTATTCTACAAGCTTCTTATCGAGCATGTTGAGGAATTACTCCCAGTTGTTTATACTCCAACCGTCGGTGAAGCTTGCCAGAAATATGGAAGCATCTTCCAGCGTCCCCAGGGTCTATTCATAAGTTTGAAAGAAAAGGGAAAGATTCTTGAAGTGTTGCGGAACTGGCCAGAGAAGAATATTCAAGTCATTGTTGTCACTGATGGAGAGCGTATCCTAGGGCTGGGGGATCTTGGCTGCCATGGGATGGGTATACCTGTTGGCAAACTTTCTCTATATACGGCACTTGGAGGAGTTCGTCCATCTGCTTGCTTGCCTGTAACCATTGATGTGGGTACAAACAATGAGAAGCTGTTGGATGATGAGTTCTACATAGGGCTCAGGCAAAAGAGGGCAACTGGGCAGGAATACGCAGAACTCCTTCAAGAATTCATGACTGCTGTGAAGCAGAATTATGGAGAGAAAGTTCTTGTTCAGTTTGAGGACTTTGCAAACCACAATGCTTTTGATCTTCTTTCGAAGTATGGCGCAACTCATCTTGTTTTTAATGATGATATTCAGGGGACAGCATCTGTGGTCCTTGCAGGACTCTTGTCATCTTTGAAGTTAGTAGGGGGATCCTTAGCTGATCACACATTTTTATTCCTTGGTGCTGGAGAGGCTGGCACTGGCATAGCGGAGCTCATAGCCCTTGAACTATCTAAGCAGACAAATGCCCCACTGGAAGAAACTCGCAAGAAGGTTTGGCTTGTGGACTCGAAGGGATTGATTGTCAGTTCCCGGTTGGAATCACTCCAACATTTTAAGAAGCCTTGGGCTCATGAACATGAACCAGTCAAGACACTTGCAGAAGCTGTTAATGCAATCAAGCCAACGGTGTTGATTGGAACGTCAGGAGTAGGAAGAACATTTACTAAAGAAGTGGTTGAGGCTATGGCCACCAACAATGAGAGACCCATTATTCTTGCTCTCTCGAACCCAACATCACAATCTGAGTGTACTGCTGAAGAAGCATATACATGGACACAGGGTCGTGCCATTTACTGTAGTGGAAGTCCATTCCCACCAGTTGAATACGAGGGAAAGGTTTACACTCCTGGCCAGTCGAATAATGCTTACATCTTCCCTGGATTTGGTCTCGGTTTATTGATGTCTGGTACCATTCGTGTTCATGATGACATGCTTCTGGCAGCCTCGGAAGCTTTGGCTTCACAGGTATCCCAGGAAGATTTTGACAAGGGACTCATATACCCTCCATTTACAAACATCAGAGAGATTTCAGCACATATTGCTGCTAAAGTAGCTGCTAAATCATATGAACTCGGTTTGGCTACACGCCTCCCCCAGCCAAAGGATCTGTTCCAGTATGCTGAAAGCTGTATGTACAGCCCAAACTACCGAAGTTTCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006090 GO:0006108 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016053 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

636

Amino Acids

69.87

Weight (kDa)

6.31

Isoelectric Point (pI)

41.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	161 - 341	7.3e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	352 - 603	3.5e-97	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79750
fragaria_vesca	FvH4_6g32330 FvH4_6g32330 FvH4_6g32330
malus_domestica	MD09G1203300.v1.1 MD17G1184200.v1.1
prunus_persica	Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1
pyrus_communis	pycom09g12010 pycom17g19390
rosa_chinensis	RchiOBHm_Chr2g0140441
rosa_laevigata	RLG00000019867
rosa_multiflora	Rmu_sc0003516.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436750 Rroxscaffold_2G00104830
rosa_rugosa	Rorug02G0362900 Rorug02G0363000 Rorug02G0363100 Rorug02G0363200 Rorug02G0363300
rosa_samantha	Rh2AG413500 Rh2BG423700 Rh2CG399700 Rh2DG433400
rosa_wichuraiana	Rw2G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	294
AasI	GACNNNNNNGTC	1 cut(s)	257
Acc65I	GGTACC	1 cut(s)	1655
AccB1I	GGYRCC	1 cut(s)	1655
AccB7I	CCANNNNNTGG	1 cut(s)	745
AccI	GTMKAC	1 cut(s)	751
AciI	CCGC	5 cut(s)	85, 126, 433, 658, 1175
AclWI	GGATC	6 cut(s)	302, 483, 735, 1110, 1123, 1860
AcoI	YGGCCR	3 cut(s)	665, 1434, 1597
AcsI	RAATTY	1 cut(s)	926
AcuI	CTGAAG	3 cut(s)	389, 464, 1524
AfaI	GTAC	4 cut(s)	835, 1498, 1657, 1883
AfiI	CCNNNNNNNGG	6 cut(s)	211, 563, 721, 745, 1631, 1849
AflIII	ACRYGT	1 cut(s)	222
AgsI	TTSAA	7 cut(s)	628, 650, 683, 923, 1102, 1193, 1571
AhdI	GACNNNNNGTC	1 cut(s)	1527
AjiI	CACGTC	1 cut(s)	223
AjnI	CCWGG	6 cut(s)	128, 266, 606, 1594, 1624, 1719
AloI	GAACNNNNNNTCC	4 cut(s)	848, 880, 948, 980
Alw21I	GWGCWC	1 cut(s)	1182
Alw26I	GTCTC	2 cut(s)	1444, 1640
AlwI	GGATC	6 cut(s)	302, 483, 735, 1110, 1123, 1860
AlwNI	CAGNNNCTG	2 cut(s)	1070, 1352
AoxI	GGCC	3 cut(s)	665, 1434, 1597
ApeKI	GCWGC	7 cut(s)	21, 150, 170, 735, 1688, 1790, 1802
ApoI	RAATTY	1 cut(s)	926
Asp700I	GAANNNNTTC	2 cut(s)	500, 918
Asp718I	GGTACC	1 cut(s)	1655
AspA2I	CCTAGG	1 cut(s)	715
AspLEI	GCGC	1 cut(s)	1028
AspS9I	GGNCC	2 cut(s)	250, 1075
AsuC2I	CCSGG	1 cut(s)	1279
AsuHPI	GGTGA	3 cut(s)	242, 421, 578
AsuII	TTCGAA	1 cut(s)	1016
AvaII	GGWCC	2 cut(s)	250, 1075
AvrII	CCTAGG	1 cut(s)	715
BalI	TGGCCA	3 cut(s)	667, 1436, 1599
BamHI	GGATCC	1 cut(s)	1115
BanI	GGYRCC	1 cut(s)	1655
BanII	GRGCYC	3 cut(s)	879, 1182, 1318
BarI	GAAGNNNNNNTAC	4 cut(s)	488, 520, 1393, 1425
BbsI	GAAGAC	1 cut(s)	365
Bbv12I	GWGCWC	1 cut(s)	1182
BbvI	GCAGC	7 cut(s)	8, 162, 182, 722, 1700, 1777, 1789
BccI	CCATC	5 cut(s)	437, 466, 695, 739, 808
BceAI	ACGGC	2 cut(s)	163, 795
BciT130I	CCWGG	6 cut(s)	130, 268, 608, 1596, 1626, 1721
BciVI	GTATCC	3 cut(s)	229, 722, 1726
BclI	TGATCA	1 cut(s)	1126
BcnI	CCSGG	1 cut(s)	1279
BcoDI	GTCTC	2 cut(s)	1444, 1640
BfaI	CTAG	1 cut(s)	716
BfmI	CTRYAG	2 cut(s)	452, 1543
BfuI	GTATCC	3 cut(s)	229, 722, 1726
BisI	GCNGC	8 cut(s)	22, 126, 151, 171, 736, 1689, 1791, 1803
BlnI	CCTAGG	1 cut(s)	715
BlsI	GCNGC	8 cut(s)	23, 127, 152, 172, 737, 1690, 1792, 1804
Bme1390I	CCNGG	7 cut(s)	130, 268, 608, 1279, 1596, 1626, 1721
Bme18I	GGWCC	2 cut(s)	250, 1075
BmeRI	GACNNNNNGTC	1 cut(s)	1527
BmgBI	CACGTC	1 cut(s)	223
BmgT120I	GGNCC	2 cut(s)	250, 1075
BmiI	GGNNCC	2 cut(s)	1117, 1657
BmrFI	CCNGG	7 cut(s)	130, 268, 608, 1279, 1596, 1626, 1721
BmrI	ACTGGG	3 cut(s)	377, 536, 906
BmsI	GCATC	3 cut(s)	343, 600, 1076
BmuI	ACTGGG	3 cut(s)	377, 536, 906
BoxI	GACNNNNGTC	1 cut(s)	1090
BpiI	GAAGAC	1 cut(s)	365
BpmI	CTGGAG	2 cut(s)	289, 1173
Bpu10I	CCTNAGC	3 cut(s)	29, 878, 1120
Bpu14I	TTCGAA	1 cut(s)	1016
BpuEI	CTTGAG	1 cut(s)	381
BpuMI	CCSGG	1 cut(s)	1279
BsaI	GGTCTC	2 cut(s)	1444, 1640
BsaWI	WCCGGW	1 cut(s)	1905
BsaXI	ACNNNNNCTCC	2 cut(s)	948, 978
Bsc4I	CCNNNNNNNGG	6 cut(s)	211, 563, 721, 745, 1631, 1849
BseBI	CCWGG	6 cut(s)	130, 268, 608, 1596, 1626, 1721
BseGI	GGATG	3 cut(s)	429, 750, 862
BseLI	CCNNNNNNNGG	6 cut(s)	211, 563, 721, 745, 1631, 1849
BseMII	CTCAG	4 cut(s)	20, 83, 892, 1482
BseRI	GAGGAG	1 cut(s)	804
BseXI	GCAGC	7 cut(s)	8, 162, 182, 722, 1700, 1777, 1789
BseYI	CCCAGC	2 cut(s)	721, 1842
Bsh1285I	CGRYCG	1 cut(s)	89
BshFI	GGCC	3 cut(s)	667, 1436, 1599
BshNI	GGYRCC	1 cut(s)	1655
BsiEI	CGRYCG	1 cut(s)	89
BsiHKAI	GWGCWC	1 cut(s)	1182
BsiSI	CCGG	2 cut(s)	1279, 1906
BslFI	GGGAC	5 cut(s)	130, 227, 588, 1075, 1752
BslI	CCNNNNNNNGG	6 cut(s)	211, 563, 721, 745, 1631, 1849
BsmAI	GTCTC	2 cut(s)	1444, 1640
BsmFI	GGGAC	5 cut(s)	130, 227, 588, 1075, 1752
BsnI	GGCC	3 cut(s)	667, 1436, 1599
Bso31I	GGTCTC	2 cut(s)	1444, 1640
Bsp119I	TTCGAA	1 cut(s)	1016
Bsp1286I	GDGCHC	3 cut(s)	879, 1182, 1318
Bsp1407I	TGTACA	1 cut(s)	1881
Bsp143I	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
Bsp19I	CCATGG	2 cut(s)	205, 739
BspACI	CCGC	5 cut(s)	85, 126, 433, 658, 1175
BspANI	GGCC	3 cut(s)	667, 1436, 1599
BspCNI	CTCAG	4 cut(s)	21, 82, 891, 1483
BspHI	TCATGA	3 cut(s)	930, 1318, 1669
BspLI	GGNNCC	2 cut(s)	1117, 1657
BspMAI	CTGCAG	1 cut(s)	456
BspPI	GGATC	6 cut(s)	302, 483, 735, 1110, 1123, 1860
BspT104I	TTCGAA	1 cut(s)	1016
BspT107I	GGYRCC	1 cut(s)	1655
BspTNI	GGTCTC	2 cut(s)	1444, 1640
BsrGI	TGTACA	1 cut(s)	1881
BssMI	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
BssNAI	GTATAC	1 cut(s)	752
BssT1I	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
Bst1107I	GTATAC	1 cut(s)	752
Bst2UI	CCWGG	6 cut(s)	130, 268, 608, 1596, 1626, 1721
Bst4CI	ACNGT	4 cut(s)	202, 562, 1375, 1544
Bst6I	CTCTTC	1 cut(s)	74
BstAPI	GCANNNNNTGC	1 cut(s)	1787
BstAUI	TGTACA	1 cut(s)	1881
BstBI	TTCGAA	1 cut(s)	1016
BstC8I	GCNNGC	5 cut(s)	244, 574, 808, 812, 1162
BstDEI	CTNAG	6 cut(s)	29, 69, 878, 1120, 1200, 1491
BstDSI	CCRYGG	2 cut(s)	205, 739
BstF5I	GGATG	3 cut(s)	429, 750, 862
BstHHI	GCGC	1 cut(s)	1028
BstKTI	GATC	8 cut(s)	6, 310, 478, 730, 1009, 1118, 1129, 1855
BstMAI	GTCTC	2 cut(s)	1444, 1640
BstMBI	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
BstMCI	CGRYCG	1 cut(s)	89
BstNI	CCWGG	6 cut(s)	130, 268, 608, 1596, 1626, 1721
BstNSI	RCATGY	3 cut(s)	367, 527, 1681
BstPAI	GACNNNNGTC	1 cut(s)	1090
BstSCI	CCNGG	7 cut(s)	128, 266, 606, 1277, 1594, 1624, 1719
BstSFI	CTRYAG	2 cut(s)	452, 1543
BstV1I	GCAGC	7 cut(s)	8, 162, 182, 722, 1700, 1777, 1789
BstV2I	GAAGAC	1 cut(s)	365
BstX2I	RGATCY	5 cut(s)	307, 475, 727, 1115, 1852
BstXI	CCANNNNNNTGG	2 cut(s)	584, 829
BstYI	RGATCY	5 cut(s)	307, 475, 727, 1115, 1852
BstZ17I	GTATAC	1 cut(s)	752
BsuI	GTATCC	3 cut(s)	229, 722, 1726
BsuRI	GGCC	3 cut(s)	667, 1436, 1599
BtgI	CCRYGG	2 cut(s)	205, 739
BtrI	CACGTC	1 cut(s)	223
BtsCI	GGATG	3 cut(s)	429, 750, 862
BtsIMutI	CAGTG	3 cut(s)	696, 1164, 1217
Cac8I	GCNNGC	5 cut(s)	244, 574, 808, 812, 1162
CaiI	CAGNNNCTG	2 cut(s)	1070, 1352
CciI	TCATGA	3 cut(s)	930, 1318, 1669
CfoI	GCGC	1 cut(s)	1028
Cfr13I	GGNCC	2 cut(s)	250, 1075
CseI	GACGC	1 cut(s)	590
Csp6I	GTAC	4 cut(s)	834, 1497, 1656, 1882
CviQI	GTAC	4 cut(s)	834, 1497, 1656, 1882
DdeI	CTNAG	6 cut(s)	29, 69, 878, 1120, 1200, 1491
DpnI	GATC	8 cut(s)	5, 309, 477, 729, 1008, 1117, 1128, 1854
DpnII	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
DrdI	GACNNNNNNGTC	1 cut(s)	257
DriI	GACNNNNNGTC	1 cut(s)	1527
DseDI	GACNNNNNNGTC	1 cut(s)	257
EaeI	YGGCCR	3 cut(s)	665, 1434, 1597
Eam1104I	CTCTTC	1 cut(s)	74
Eam1105I	GACNNNNNGTC	1 cut(s)	1527
EarI	CTCTTC	1 cut(s)	74
EciI	GGCGGA	1 cut(s)	422
Ecl136II	GAGCTC	1 cut(s)	1180
Eco130I	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
Eco24I	GRGCYC	3 cut(s)	879, 1182, 1318
Eco31I	GGTCTC	2 cut(s)	1444, 1640
Eco47I	GGWCC	2 cut(s)	250, 1075
Eco53kI	GAGCTC	1 cut(s)	1180
Eco57I	CTGAAG	3 cut(s)	389, 464, 1524
EcoICRI	GAGCTC	1 cut(s)	1180
EcoRI	GAATTC	1 cut(s)	926
EcoRII	CCWGG	6 cut(s)	128, 266, 606, 1594, 1624, 1719
EcoT14I	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
EcoT38I	GRGCYC	3 cut(s)	879, 1182, 1318
ErhI	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
FaqI	GGGAC	5 cut(s)	130, 227, 588, 1075, 1752
FauNDI	CATATG	1 cut(s)	1813
FbaI	TGATCA	1 cut(s)	1126
FblI	GTMKAC	1 cut(s)	751
Fnu4HI	GCNGC	8 cut(s)	22, 126, 151, 171, 736, 1689, 1791, 1803
FokI	GGATG	3 cut(s)	416, 757, 869
FriOI	GRGCYC	3 cut(s)	879, 1182, 1318
Fsp4HI	GCNGC	8 cut(s)	22, 126, 151, 171, 736, 1689, 1791, 1803
FspBI	CTAG	1 cut(s)	716
GlaI	GCGC	1 cut(s)	1027
GluI	GCNGC	8 cut(s)	22, 126, 151, 171, 736, 1689, 1791, 1803
GsaI	CCCAGC	2 cut(s)	725, 1846
GsuI	CTGGAG	2 cut(s)	289, 1173
HaeIII	GGCC	3 cut(s)	667, 1436, 1599
HapII	CCGG	2 cut(s)	1279, 1906
HgaI	GACGC	1 cut(s)	590
HhaI	GCGC	1 cut(s)	1028
Hin6I	GCGC	1 cut(s)	1026
HinP1I	GCGC	1 cut(s)	1026
HindIII	AAGCTT	3 cut(s)	509, 570, 1698
HinfI	GANTC	5 cut(s)	643, 1086, 1253, 1286, 1740
HpaII	CCGG	2 cut(s)	1279, 1906
HphI	GGTGA	3 cut(s)	242, 421, 578
Hpy166II	GTNNAC	4 cut(s)	752, 1252, 1497, 1588
Hpy188I	TCNGA	4 cut(s)	340, 1492, 1696, 1769
Hpy8I	GTNNAC	4 cut(s)	752, 1252, 1497, 1588
Hpy99I	CGWCG	1 cut(s)	566
HpyAV	CCTTC	4 cut(s)	343, 929, 1231, 1252
HpyCH4III	ACNGT	4 cut(s)	202, 562, 1375, 1544
HpyCH4IV	ACGT	2 cut(s)	222, 1388
HpyCH4V	TGCA	6 cut(s)	284, 454, 989, 1082, 1346, 1361
HpyF3I	CTNAG	6 cut(s)	29, 69, 878, 1120, 1200, 1491
HpySE526I	ACGT	2 cut(s)	222, 1388
HspAI	GCGC	1 cut(s)	1026
KpnI	GGTACC	1 cut(s)	1659
Ksp22I	TGATCA	1 cut(s)	1126
Kzo9I	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
LmnI	GCTCC	1 cut(s)	1177
Lsp1109I	GCAGC	7 cut(s)	8, 162, 182, 722, 1700, 1777, 1789
LweI	GCATC	3 cut(s)	343, 600, 1076
MaeI	CTAG	1 cut(s)	716
MaeII	ACGT	2 cut(s)	222, 1388
MaeIII	GTNAC	3 cut(s)	259, 694, 817
MalI	GATC	8 cut(s)	5, 309, 477, 729, 1008, 1117, 1128, 1854
MboI	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
MflI	RGATCY	5 cut(s)	307, 475, 727, 1115, 1852
MhlI	GDGCHC	3 cut(s)	879, 1182, 1318
MlsI	TGGCCA	3 cut(s)	667, 1436, 1599
MluCI	AATT	5 cut(s)	52, 295, 533, 926, 949
MluNI	TGGCCA	3 cut(s)	667, 1436, 1599
MlyI	GAGTC	3 cut(s)	1080, 1247, 1734
MmeI	TCCRAC	4 cut(s)	581, 834, 1263, 1318
Mox20I	TGGCCA	3 cut(s)	667, 1436, 1599
MroXI	GAANNNNTTC	2 cut(s)	500, 918
MscI	TGGCCA	3 cut(s)	667, 1436, 1599
MseI	TTAA	3 cut(s)	1044, 1302, 1356
MslI	CAYNNNNRTG	4 cut(s)	158, 827, 1323, 1516
Msp20I	TGGCCA	3 cut(s)	667, 1436, 1599
MspI	CCGG	2 cut(s)	1279, 1906
MspR9I	CCNGG	7 cut(s)	130, 268, 608, 1279, 1596, 1626, 1721
MvaI	CCWGG	6 cut(s)	130, 268, 608, 1596, 1626, 1721
NciI	CCSGG	1 cut(s)	1279
NcoI	CCATGG	2 cut(s)	205, 739
NdeI	CATATG	1 cut(s)	1813
NdeII	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
NlaIV	GGNNCC	2 cut(s)	1117, 1657
NmuCI	GTSAC	2 cut(s)	259, 694
NspI	RCATGY	3 cut(s)	367, 527, 1681
NspV	TTCGAA	1 cut(s)	1016
PagI	TCATGA	3 cut(s)	930, 1318, 1669
PasI	CCCWGGG	1 cut(s)	607
PdmI	GAANNNNTTC	2 cut(s)	500, 918
PfeI	GAWTC	2 cut(s)	643, 1286
PflMI	CCANNNNNTGG	1 cut(s)	745
PkrI	GCNGC	8 cut(s)	23, 127, 152, 172, 737, 1690, 1792, 1804
PleI	GAGTC	3 cut(s)	1080, 1247, 1734
PpsI	GAGTC	3 cut(s)	1080, 1247, 1734
PshAI	GACNNNNGTC	1 cut(s)	1090
PsiI	TTATAA	1 cut(s)	294
Psp124BI	GAGCTC	1 cut(s)	1182
Psp6I	CCWGG	6 cut(s)	128, 266, 606, 1594, 1624, 1719
PspFI	CCCAGC	2 cut(s)	721, 1842
PspGI	CCWGG	6 cut(s)	128, 266, 606, 1594, 1624, 1719
PspN4I	GGNNCC	2 cut(s)	1117, 1657
PspPI	GGNCC	2 cut(s)	250, 1075
PstI	CTGCAG	1 cut(s)	456
PstNI	CAGNNNCTG	2 cut(s)	1070, 1352
PsuI	RGATCY	5 cut(s)	307, 475, 727, 1115, 1852
RsaI	GTAC	4 cut(s)	835, 1498, 1657, 1883
RsaNI	GTAC	4 cut(s)	834, 1497, 1656, 1882
RseI	CAYNNNNRTG	4 cut(s)	158, 827, 1323, 1516
SacI	GAGCTC	1 cut(s)	1182
SaqAI	TTAA	3 cut(s)	1044, 1302, 1356
SatI	GCNGC	8 cut(s)	22, 126, 151, 171, 736, 1689, 1791, 1803
Sau3AI	GATC	8 cut(s)	3, 307, 475, 727, 1006, 1115, 1126, 1852
Sau96I	GGNCC	2 cut(s)	250, 1075
SchI	GAGTC	3 cut(s)	1080, 1247, 1734
ScrFI	CCNGG	7 cut(s)	130, 268, 608, 1279, 1596, 1626, 1721
SduI	GDGCHC	3 cut(s)	879, 1182, 1318
SfaNI	GCATC	3 cut(s)	343, 600, 1076
SfcI	CTRYAG	2 cut(s)	452, 1543
SfuI	TTCGAA	1 cut(s)	1016
SinI	GGWCC	2 cut(s)	250, 1075
SmiMI	CAYNNNNRTG	4 cut(s)	158, 827, 1323, 1516
SmlI	CTYRAG	1 cut(s)	396
SmoI	CTYRAG	1 cut(s)	396
Sse9I	AATT	5 cut(s)	52, 295, 533, 926, 949
SsiI	CCGC	5 cut(s)	85, 126, 433, 658, 1175
SspI	AATATT	1 cut(s)	679
SspMI	CTAG	1 cut(s)	716
SstI	GAGCTC	1 cut(s)	1182
StyD4I	CCNGG	7 cut(s)	128, 266, 606, 1277, 1594, 1624, 1719
StyI	CCWWGG	5 cut(s)	205, 715, 739, 1144, 1310
TaaI	ACNGT	4 cut(s)	202, 562, 1375, 1544
TaiI	ACGT	2 cut(s)	225, 1391
TaqI	TCGA	5 cut(s)	519, 1016, 1256, 1472, 1604
TasI	AATT	5 cut(s)	52, 295, 533, 926, 949
TatI	WGTACW	2 cut(s)	1496, 1881
TauI	GCSGC	1 cut(s)	128
TfiI	GAWTC	2 cut(s)	643, 1286
Tru1I	TTAA	3 cut(s)	1044, 1302, 1356
Tru9I	TTAA	3 cut(s)	1044, 1302, 1356
TscAI	CASTG	3 cut(s)	703, 1171, 1224
TseFI	GTSAC	2 cut(s)	259, 694
TseI	GCWGC	7 cut(s)	21, 150, 170, 735, 1688, 1790, 1802
Tsp45I	GTSAC	2 cut(s)	259, 694
TspDTI	ATGAA	6 cut(s)	607, 919, 1335, 1341, 1658, 1830
TspRI	CASTG	3 cut(s)	703, 1171, 1224
Van91I	CCANNNNNTGG	1 cut(s)	745
VpaK11BI	GGWCC	2 cut(s)	250, 1075
XapI	RAATTY	1 cut(s)	926
XceI	RCATGY	3 cut(s)	367, 527, 1681
XcmI	CCANNNNNNNNNTGG	1 cut(s)	365
XmaJI	CCTAGG	1 cut(s)	715
XmiI	GTMKAC	1 cut(s)	751
XmnI	GAANNNNTTC	2 cut(s)	500, 918
XspI	CTAG	1 cut(s)	716

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.