Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0006459

Physical Location & Seq

Genomic Coordinates Reverse (-)

19035161 .. 19041370

Gene Structure 6210 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1203300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1926 bp

ATGATGTCCTTGAATAGAAGCTGTTTTCTGGGGAACCCGGCCGTTGCTGGGTCTTCAAGCCCGTTCTCTCAGAGCCAGAAGAGGCAGCCAGCGTCGCTCAAAGTGGTGGCTTTGATCCCAAAAGCCCGGACAGGTGACCGAAATGGCAGCGTCGTGATGGAGAGCACATTGCAGGAGGTGAAGACCGAGTCCGAGGTGGTTGAGCTGAAATCCACCGTCCATGGTGGGGTAAAAGACGTGTACGGTGAGGATACTGCCACCGAGGACCAACTTGTCACACCCTGGAGTGTCTCGGTTGCTAGTGGATATACGTTGATACGATCTCCACACCACAACAAAGGTCTTGCCTTCACAGAGAAAGAGAGAGATGCCCACTACTTGCGTGGCCTTCTTCCCCCAGTTGTTATTTCTCAAGAACTTCAGGTTAAGAAAATGATAAATAGTATCCGTCAATATCAAGTTCCACTGCAGAAGTATATTGCCATGATGGATCTTCAGGGGAGAAATGAAAAGCTATTCTACAAGCTTCTTATTGAGCATGTTGAGGAATTACTTCCAGTTGTCTATACTCCTACTGTCGGTGAAGCTTGCCAGAAATATGGAAGCATATTTACGCAACCACAGGGTCTTTTCATAAGTTTGAAAGAAAAGGGCAAGATTCTTGAAGTATTGAGGAACTGGCCTGAGCAGAATATTCAAGTCATTGTTGTCACTGATGGAGAGCGGATCCTAGGGCTCGGGGATCTTGGCTCTCATGGGATGGGTATACCTGTGGGTAAACTTGCTCTATACACGGCACTTGGAGGAGTTCGTCCTTCTGCTTGCTTGCCTGTAACCATTGATGTTGGTACAAACAATGAGAAGCTGTTGAATGATGAGTTTTACATAGGGCTGAGGCAAAAGAGGGCTACTGGGCAGGAATATGCAGAACTCCTGAATGAATTCATGACTGCAGTCAAGCAGAATTACGGGGAGAAAGTTCTTGTTCAGTTTGAAGACTTTGCAAACCACAATGCTTTTGATCTGCTCGCAAAGTATGCCTCAACCCATCTTGTTTTTAACGATGACATTCAGGGGACAGCATCGGTGGTTCTTGCAGGGCTTCTTGCAGCTTTGAAGTTAGTAAAGGGATCCTTATCTGAACACAGATTTTTATTCCTTGGTGCTGGAGAGGCCGGCACTGGCATAGCAGAACTCATAGCCCTTGAAATTTCCAAGCAGACAAATACCCCAGTGGAAGAGACCCGCAAGAACATTTGGCTTGTGGACTCAAAGGGATTGATTGTCAGTTCTCGGTTGGATTCACTCCAACACTTTAAAAAGCCCTGGGCTCATGAACATGAACCGGTCGGGGAACTTGTAGATGCTGTTAAGTCAATCAAGCCAACAGTATTAATTGGAACGTCAGGTGTAGGAAGAACATTCACTAAAGAAGTGGTTGAGGCTATGGCTTCCATGAATGAGAGACCTATTATTCTCGCTCTTTCCAATCCAACATCACAATCTGAATGTACTGCGGAAGAAGCATATACATGGACTGAGGGTCGTGCCATATATTGTAGTGGAAGCCCATTCCCGCCAGTTGAATATAATGGGAAGGTTTACTATCCTGGCCAGGCAAACAATGCATACATCTTCCCTGGATTCGGTCTGGGTTTAATAATGTCTGGTACTATCCGTGTTCACGACGACATGCTTCTGGCAGCTTCGGAAGCGTTGGCTTCAATGGTGACCCAGGAAGACTTTGACAAGGGACTCATATACCCTCCATTCACAAACATCAGAAAGATTTCGGCGCATATTGCTGCTAAAGTAGCTGCGAAATCATATGAACTTGGTTTGGCCACCCGCCTCCCCGAACCAAGAGATCTGGAGAAGTATGCTGAAAGCTGTATGTACAGCCCTTCCTATCGAAGTTTCCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004470 GO:0004473 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006090 GO:0006108 GO:0006629 GO:0006631 GO:0006633 GO:0008150 GO:0008152 GO:0008270 GO:0008610 GO:0009058 GO:0009507 GO:0009536 GO:0009987 GO:0016043 GO:0016053 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0019752 GO:0022607 GO:0032787 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046394 GO:0046872 GO:0046914 GO:0046983 GO:0050897 GO:0051259 GO:0051260 GO:0051262 GO:0051289 GO:0055114 GO:0065003 GO:0071704 GO:0071840 GO:0072330 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

642

Amino Acids

70.5

Weight (kDa)

6.21

Isoelectric Point (pI)

44.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	166 - 346	4.7e-78	Malic enzyme, N-terminal domain
Malic_M	PF03949	357 - 608	4.1e-98	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006459)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79750
fragaria_vesca	FvH4_6g32330 FvH4_6g32330 FvH4_6g32330
malus_domestica	MD09G1203300.v1.1 MD17G1184200.v1.1
prunus_persica	Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1 Prupe.3G059400_v2.0.a1
pyrus_communis	pycom09g12010 pycom17g19390
rosa_chinensis	RchiOBHm_Chr2g0140441
rosa_laevigata	RLG00000019867
rosa_multiflora	Rmu_sc0003516.1_g000006
rosa_roxburghii	Rroxscaffold_153G00436750 Rroxscaffold_2G00104830
rosa_rugosa	Rorug02G0362900 Rorug02G0363000 Rorug02G0363100 Rorug02G0363200 Rorug02G0363300
rosa_samantha	Rh2AG413500 Rh2BG423700 Rh2CG399700 Rh2DG433400
rosa_wichuraiana	Rw2G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	272
AccBSI	CCGCTC	1 cut(s)	724
AccI	GTMKAC	1 cut(s)	766
AciI	CCGC	5 cut(s)	724, 1246, 1517, 1577, 1849
AclWI	GGATC	7 cut(s)	109, 498, 721, 734, 750, 1125, 1138
AcoI	YGGCCR	3 cut(s)	39, 1612, 1842
AcsI	RAATTY	2 cut(s)	941, 1209
AcuI	CTGAAG	2 cut(s)	404, 479
AfaI	GTAC	5 cut(s)	242, 850, 1513, 1672, 1898
AfiI	CCNNNNNNNGG	4 cut(s)	48, 226, 578, 1646
AflIII	ACRYGT	1 cut(s)	237
AgeI	ACCGGT	1 cut(s)	1345
AhdI	GACNNNNNGTC	1 cut(s)	1542
AjiI	CACGTC	1 cut(s)	238
AjnI	CCWGG	6 cut(s)	281, 1325, 1609, 1614, 1639, 1734
Alw21I	GWGCWC	1 cut(s)	167
Alw26I	GTCTC	3 cut(s)	295, 1235, 1459
AlwI	GGATC	7 cut(s)	109, 498, 721, 734, 750, 1125, 1138
Ama87I	CYCGRG	1 cut(s)	737
AoxI	GGCC	6 cut(s)	39, 385, 680, 1173, 1612, 1842
ApeKI	GCWGC	6 cut(s)	85, 147, 1109, 1703, 1805, 1817
ApoI	RAATTY	2 cut(s)	941, 1209
AseI	ATTAAT	1 cut(s)	1394
AsiGI	ACCGGT	1 cut(s)	1345
Asp700I	GAANNNNTTC	3 cut(s)	552, 941, 1789
AspA2I	CCTAGG	1 cut(s)	730
AspLEI	GCGC	1 cut(s)	1798
AspS9I	GGNCC	1 cut(s)	265
AsuC2I	CCSGG	2 cut(s)	38, 127
AsuHPI	GGTGA	5 cut(s)	146, 190, 257, 593, 1741
AvaI	CYCGRG	1 cut(s)	737
AvaII	GGWCC	1 cut(s)	265
AvrII	CCTAGG	1 cut(s)	730
BalI	TGGCCA	2 cut(s)	1614, 1844
BamHI	GGATCC	2 cut(s)	726, 1130
BanII	GRGCYC	2 cut(s)	738, 1333
BarI	GAAGNNNNNNTAC	2 cut(s)	595, 627
BbsI	GAAGAC	4 cut(s)	45, 188, 1002, 1746
Bbv12I	GWGCWC	1 cut(s)	167
BbvCI	CCTCAGC	1 cut(s)	893
BbvI	GCAGC	6 cut(s)	97, 159, 1121, 1715, 1792, 1804
BccI	CCATC	5 cut(s)	151, 481, 710, 754, 1056
BceAI	ACGGC	2 cut(s)	26, 810
BciT130I	CCWGG	6 cut(s)	283, 1327, 1611, 1616, 1641, 1736
BciVI	GTATCC	2 cut(s)	244, 455
BcnI	CCSGG	2 cut(s)	38, 127
BcoDI	GTCTC	3 cut(s)	295, 1235, 1459
BfaI	CTAG	2 cut(s)	300, 731
BfmI	CTRYAG	2 cut(s)	467, 951
BfuI	GTATCC	2 cut(s)	244, 455
BglII	AGATCT	1 cut(s)	1867
BisI	GCNGC	6 cut(s)	86, 148, 1110, 1704, 1806, 1818
BlnI	CCTAGG	1 cut(s)	730
BlsI	GCNGC	6 cut(s)	87, 149, 1111, 1705, 1807, 1819
Bme1390I	CCNGG	8 cut(s)	38, 127, 283, 1327, 1611, 1616, 1641, 1736
Bme18I	GGWCC	1 cut(s)	265
BmeRI	GACNNNNNGTC	1 cut(s)	1542
BmeT110I	CYCGRG	1 cut(s)	737
BmgBI	CACGTC	1 cut(s)	238
BmgT120I	GGNCC	1 cut(s)	265
BmiI	GGNNCC	3 cut(s)	35, 728, 1132
BmrFI	CCNGG	8 cut(s)	38, 127, 283, 1327, 1611, 1616, 1641, 1736
BmrI	ACTGGG	3 cut(s)	392, 921, 1226
BmsI	GCATC	3 cut(s)	358, 1091, 1354
BmuI	ACTGGG	3 cut(s)	392, 921, 1226
BoxI	GACNNNNGTC	1 cut(s)	953
BpiI	GAAGAC	4 cut(s)	45, 188, 1002, 1746
BpmI	CTGGAG	3 cut(s)	304, 1188, 1892
Bpu10I	CCTNAGC	2 cut(s)	684, 893
BpuEI	CTTGAG	1 cut(s)	396
BpuMI	CCSGG	2 cut(s)	38, 127
BsaBI	GATNNNNATC	1 cut(s)	1135
BsaI	GGTCTC	2 cut(s)	1235, 1459
BsaWI	WCCGGW	2 cut(s)	1345, 1920
Bsc4I	CCNNNNNNNGG	4 cut(s)	48, 226, 578, 1646
Bse118I	RCCGGY	2 cut(s)	1175, 1345
Bse1I	ACTGG	7 cut(s)	398, 557, 683, 916, 1186, 1232, 1580
Bse3DI	GCAATG	1 cut(s)	167
Bse8I	GATNNNNATC	1 cut(s)	1135
BseBI	CCWGG	6 cut(s)	283, 1327, 1611, 1616, 1641, 1736
BseGI	GGATG	1 cut(s)	765
BseJI	GATNNNNATC	1 cut(s)	1135
BseLI	CCNNNNNNNGG	4 cut(s)	48, 226, 578, 1646
BseMI	GCAATG	1 cut(s)	167
BseMII	CTCAG	4 cut(s)	83, 675, 884, 1530
BseNI	ACTGG	7 cut(s)	398, 557, 683, 916, 1186, 1232, 1580
BseRI	GAGGAG	1 cut(s)	819
BseX3I	CGGCCG	1 cut(s)	39
BseXI	GCAGC	6 cut(s)	97, 159, 1121, 1715, 1792, 1804
BseYI	CCCAGC	1 cut(s)	47
Bsh1285I	CGRYCG	2 cut(s)	42, 1350
BshFI	GGCC	6 cut(s)	41, 387, 682, 1175, 1614, 1844
BshTI	ACCGGT	1 cut(s)	1345
BsiEI	CGRYCG	2 cut(s)	42, 1350
BsiHKAI	GWGCWC	1 cut(s)	167
BsiHKCI	CYCGRG	1 cut(s)	737
BsiSI	CCGG	5 cut(s)	38, 127, 1176, 1346, 1921
BslFI	GGGAC	2 cut(s)	1090, 1767
BslI	CCNNNNNNNGG	4 cut(s)	48, 226, 578, 1646
BsmAI	GTCTC	3 cut(s)	295, 1235, 1459
BsmFI	GGGAC	2 cut(s)	1090, 1767
BsnI	GGCC	6 cut(s)	41, 387, 682, 1175, 1614, 1844
Bso31I	GGTCTC	2 cut(s)	1235, 1459
BsoBI	CYCGRG	1 cut(s)	737
Bsp1286I	GDGCHC	3 cut(s)	167, 738, 1333
Bsp1407I	TGTACA	1 cut(s)	1896
Bsp143I	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
Bsp19I	CCATGG	1 cut(s)	220
BspACI	CCGC	5 cut(s)	724, 1246, 1517, 1577, 1849
BspANI	GGCC	6 cut(s)	41, 387, 682, 1175, 1614, 1844
BspCNI	CTCAG	4 cut(s)	82, 676, 885, 1531
BspHI	TCATGA	2 cut(s)	945, 1333
BspLI	GGNNCC	3 cut(s)	35, 728, 1132
BspMAI	CTGCAG	2 cut(s)	471, 955
BspPI	GGATC	7 cut(s)	109, 498, 721, 734, 750, 1125, 1138
BspTNI	GGTCTC	2 cut(s)	1235, 1459
BsrBI	CCGCTC	1 cut(s)	724
BsrDI	GCAATG	1 cut(s)	167
BsrFI	RCCGGY	2 cut(s)	1175, 1345
BsrGI	TGTACA	1 cut(s)	1896
BsrI	ACTGG	7 cut(s)	398, 557, 683, 916, 1186, 1232, 1580
BssAI	RCCGGY	2 cut(s)	1175, 1345
BssMI	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
BssNAI	GTATAC	1 cut(s)	767
BssT1I	CCWWGG	3 cut(s)	220, 730, 1159
Bst1107I	GTATAC	1 cut(s)	767
Bst2UI	CCWGG	6 cut(s)	283, 1327, 1611, 1616, 1641, 1736
Bst4CI	ACNGT	4 cut(s)	217, 245, 577, 1390
Bst6I	CTCTTC	2 cut(s)	74, 1233
BstAPI	GCANNNNNTGC	2 cut(s)	1037, 1625
BstAUI	TGTACA	1 cut(s)	1896
BstC8I	GCNNGC	6 cut(s)	90, 589, 823, 827, 1029, 1177
BstDEI	CTNAG	4 cut(s)	69, 684, 893, 1539
BstDSI	CCRYGG	1 cut(s)	220
BstEII	GGTNACC	2 cut(s)	134, 1729
BstF5I	GGATG	1 cut(s)	765
BstHHI	GCGC	1 cut(s)	1798
BstKTI	GATC	8 cut(s)	117, 323, 493, 729, 745, 1024, 1133, 1870
BstMAI	GTCTC	3 cut(s)	295, 1235, 1459
BstMBI	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
BstMCI	CGRYCG	2 cut(s)	42, 1350
BstMWI	GCNNNNNNNGC	8 cut(s)	94, 1037, 1172, 1523, 1625, 1712, 1802, 1814
BstNI	CCWGG	6 cut(s)	283, 1327, 1611, 1616, 1641, 1736
BstNSI	RCATGY	2 cut(s)	542, 1696
BstPAI	GACNNNNGTC	1 cut(s)	953
BstPI	GGTNACC	2 cut(s)	134, 1729
BstSCI	CCNGG	8 cut(s)	36, 125, 281, 1325, 1609, 1614, 1639, 1734
BstSFI	CTRYAG	2 cut(s)	467, 951
BstV1I	GCAGC	6 cut(s)	97, 159, 1121, 1715, 1792, 1804
BstV2I	GAAGAC	4 cut(s)	45, 188, 1002, 1746
BstX2I	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
BstXI	CCANNNNNNTGG	1 cut(s)	599
BstYI	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
BstZ17I	GTATAC	1 cut(s)	767
BstZI	CGGCCG	1 cut(s)	39
BsuI	GTATCC	2 cut(s)	244, 455
BsuRI	GGCC	6 cut(s)	41, 387, 682, 1175, 1614, 1844
BtgI	CCRYGG	1 cut(s)	220
BtrI	CACGTC	1 cut(s)	238
BtsCI	GGATG	1 cut(s)	765
BtsI	GCAGTG	1 cut(s)	464
BtsIMutI	CAGTG	4 cut(s)	464, 711, 1179, 1239
Cac8I	GCNNGC	6 cut(s)	90, 589, 823, 827, 1029, 1177
CciI	TCATGA	2 cut(s)	945, 1333
CfoI	GCGC	1 cut(s)	1798
Cfr10I	RCCGGY	2 cut(s)	1175, 1345
Cfr13I	GGNCC	1 cut(s)	265
CseI	GACGC	2 cut(s)	81, 139
Csp6I	GTAC	5 cut(s)	241, 849, 1512, 1671, 1897
CspAI	ACCGGT	1 cut(s)	1345
CviQI	GTAC	5 cut(s)	241, 849, 1512, 1671, 1897
DdeI	CTNAG	4 cut(s)	69, 684, 893, 1539
DpnI	GATC	8 cut(s)	116, 322, 492, 728, 744, 1023, 1132, 1869
DpnII	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
DraI	TTTAAA	1 cut(s)	1318
DrdI	GACNNNNNNGTC	1 cut(s)	272
DriI	GACNNNNNGTC	1 cut(s)	1542
DseDI	GACNNNNNNGTC	1 cut(s)	272
EaeI	YGGCCR	3 cut(s)	39, 1612, 1842
EagI	CGGCCG	1 cut(s)	39
Eam1104I	CTCTTC	2 cut(s)	74, 1233
Eam1105I	GACNNNNNGTC	1 cut(s)	1542
EarI	CTCTTC	2 cut(s)	74, 1233
EclXI	CGGCCG	1 cut(s)	39
Eco130I	CCWWGG	3 cut(s)	220, 730, 1159
Eco24I	GRGCYC	2 cut(s)	738, 1333
Eco31I	GGTCTC	2 cut(s)	1235, 1459
Eco47I	GGWCC	1 cut(s)	265
Eco52I	CGGCCG	1 cut(s)	39
Eco57I	CTGAAG	2 cut(s)	404, 479
Eco88I	CYCGRG	1 cut(s)	737
Eco91I	GGTNACC	2 cut(s)	134, 1729
EcoO65I	GGTNACC	2 cut(s)	134, 1729
EcoRI	GAATTC	1 cut(s)	941
EcoRII	CCWGG	6 cut(s)	281, 1325, 1609, 1614, 1639, 1734
EcoT14I	CCWWGG	3 cut(s)	220, 730, 1159
EcoT22I	ATGCAT	1 cut(s)	1630
EcoT38I	GRGCYC	2 cut(s)	738, 1333
ErhI	CCWWGG	3 cut(s)	220, 730, 1159
FalI	AAGNNNNNCTT	2 cut(s)	1118, 1150
FaqI	GGGAC	2 cut(s)	1090, 1767
FauI	CCCGC	3 cut(s)	1253, 1584, 1856
FauNDI	CATATG	1 cut(s)	1828
FblI	GTMKAC	1 cut(s)	766
Fnu4HI	GCNGC	6 cut(s)	86, 148, 1110, 1704, 1806, 1818
FokI	GGATG	1 cut(s)	772
FriOI	GRGCYC	2 cut(s)	738, 1333
Fsp4HI	GCNGC	6 cut(s)	86, 148, 1110, 1704, 1806, 1818
FspBI	CTAG	2 cut(s)	300, 731
GlaI	GCGC	1 cut(s)	1797
GluI	GCNGC	6 cut(s)	86, 148, 1110, 1704, 1806, 1818
GsaI	CCCAGC	1 cut(s)	51
GsuI	CTGGAG	3 cut(s)	304, 1188, 1892
HaeIII	GGCC	6 cut(s)	41, 387, 682, 1175, 1614, 1844
HapII	CCGG	5 cut(s)	38, 127, 1176, 1346, 1921
HgaI	GACGC	2 cut(s)	81, 139
HhaI	GCGC	1 cut(s)	1798
Hin6I	GCGC	1 cut(s)	1796
HinP1I	GCGC	1 cut(s)	1796
HindIII	AAGCTT	2 cut(s)	524, 585
HinfI	GANTC	6 cut(s)	188, 658, 1268, 1301, 1644, 1755
HpaII	CCGG	5 cut(s)	38, 127, 1176, 1346, 1921
HphI	GGTGA	5 cut(s)	146, 190, 257, 593, 1741
Hpy166II	GTNNAC	6 cut(s)	241, 767, 779, 1267, 1603, 1684
Hpy188I	TCNGA	6 cut(s)	72, 193, 1141, 1507, 1711, 1784
Hpy188III	TCNNGA	8 cut(s)	154, 413, 662, 934, 946, 1334, 1685, 1871
Hpy8I	GTNNAC	6 cut(s)	241, 767, 779, 1267, 1603, 1684
Hpy99I	CGWCG	3 cut(s)	97, 155, 1691
HpyAV	CCTTC	5 cut(s)	358, 398, 825, 1591, 1914
HpyCH4III	ACNGT	4 cut(s)	217, 245, 577, 1390
HpyCH4IV	ACGT	3 cut(s)	237, 311, 1403
HpyCH4V	TGCA	8 cut(s)	172, 469, 926, 953, 1004, 1097, 1109, 1628
HpyF10VI	GCNNNNNNNGC	8 cut(s)	94, 1037, 1172, 1523, 1625, 1712, 1802, 1814
HpyF3I	CTNAG	4 cut(s)	69, 684, 893, 1539
HpySE526I	ACGT	3 cut(s)	237, 311, 1403
HspAI	GCGC	1 cut(s)	1796
KroI	GCCGGC	1 cut(s)	1175
KroNI	GCCGGC	1 cut(s)	1177
Kzo9I	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
Lsp1109I	GCAGC	6 cut(s)	97, 159, 1121, 1715, 1792, 1804
LweI	GCATC	3 cut(s)	358, 1091, 1354
MaeI	CTAG	2 cut(s)	300, 731
MaeII	ACGT	3 cut(s)	237, 311, 1403
MaeIII	GTNAC	5 cut(s)	134, 274, 709, 832, 1729
MalI	GATC	8 cut(s)	116, 322, 492, 728, 744, 1023, 1132, 1869
MbiI	CCGCTC	1 cut(s)	724
MboI	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
MflI	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
MhlI	GDGCHC	3 cut(s)	167, 738, 1333
MlsI	TGGCCA	2 cut(s)	1614, 1844
MluCI	AATT	5 cut(s)	548, 941, 964, 1209, 1395
MluNI	TGGCCA	2 cut(s)	1614, 1844
MlyI	GAGTC	3 cut(s)	197, 1262, 1749
MmeI	TCCRAC	3 cut(s)	1278, 1333, 1517
Mox20I	TGGCCA	2 cut(s)	1614, 1844
Mph1103I	ATGCAT	1 cut(s)	1630
MroNI	GCCGGC	1 cut(s)	1175
MroXI	GAANNNNTTC	3 cut(s)	552, 941, 1789
MscI	TGGCCA	2 cut(s)	1614, 1844
MseI	TTAA	6 cut(s)	426, 1059, 1317, 1371, 1394, 1658
MslI	CAYNNNNRTG	2 cut(s)	1338, 1531
Msp20I	TGGCCA	2 cut(s)	1614, 1844
MspI	CCGG	5 cut(s)	38, 127, 1176, 1346, 1921
MspR9I	CCNGG	8 cut(s)	38, 127, 283, 1327, 1611, 1616, 1641, 1736
MvaI	CCWGG	6 cut(s)	283, 1327, 1611, 1616, 1641, 1736
MwoI	GCNNNNNNNGC	8 cut(s)	94, 1037, 1172, 1523, 1625, 1712, 1802, 1814
NaeI	GCCGGC	1 cut(s)	1177
NciI	CCSGG	2 cut(s)	38, 127
NcoI	CCATGG	1 cut(s)	220
NdeI	CATATG	1 cut(s)	1828
NdeII	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
NgoMIV	GCCGGC	1 cut(s)	1175
NlaIV	GGNNCC	3 cut(s)	35, 728, 1132
NmuCI	GTSAC	4 cut(s)	134, 274, 709, 1729
NsiI	ATGCAT	1 cut(s)	1630
NspI	RCATGY	2 cut(s)	542, 1696
PagI	TCATGA	2 cut(s)	945, 1333
PasI	CCCWGGG	1 cut(s)	1326
PdiI	GCCGGC	1 cut(s)	1177
PdmI	GAANNNNTTC	3 cut(s)	552, 941, 1789
PfeI	GAWTC	3 cut(s)	658, 1301, 1644
PflFI	GACNNNGTC	1 cut(s)	187
PinAI	ACCGGT	1 cut(s)	1345
PkrI	GCNGC	6 cut(s)	87, 149, 1111, 1705, 1807, 1819
PleI	GAGTC	3 cut(s)	196, 1262, 1749
PpsI	GAGTC	3 cut(s)	196, 1262, 1749
PshAI	GACNNNNGTC	1 cut(s)	953
PshBI	ATTAAT	1 cut(s)	1394
Psp6I	CCWGG	6 cut(s)	281, 1325, 1609, 1614, 1639, 1734
PspEI	GGTNACC	2 cut(s)	134, 1729
PspFI	CCCAGC	1 cut(s)	47
PspGI	CCWGG	6 cut(s)	281, 1325, 1609, 1614, 1639, 1734
PspN4I	GGNNCC	3 cut(s)	35, 728, 1132
PspPI	GGNCC	1 cut(s)	265
PstI	CTGCAG	2 cut(s)	471, 955
PsuI	RGATCY	5 cut(s)	490, 726, 742, 1130, 1867
PsyI	GACNNNGTC	1 cut(s)	187
RsaI	GTAC	5 cut(s)	242, 850, 1513, 1672, 1898
RsaNI	GTAC	5 cut(s)	241, 849, 1512, 1671, 1897
RseI	CAYNNNNRTG	2 cut(s)	1338, 1531
SaqAI	TTAA	6 cut(s)	426, 1059, 1317, 1371, 1394, 1658
SatI	GCNGC	6 cut(s)	86, 148, 1110, 1704, 1806, 1818
Sau3AI	GATC	8 cut(s)	114, 320, 490, 726, 742, 1021, 1130, 1867
Sau96I	GGNCC	1 cut(s)	265
SchI	GAGTC	3 cut(s)	197, 1262, 1749
ScrFI	CCNGG	8 cut(s)	38, 127, 283, 1327, 1611, 1616, 1641, 1736
SduI	GDGCHC	3 cut(s)	167, 738, 1333
SfaNI	GCATC	3 cut(s)	358, 1091, 1354
SfcI	CTRYAG	2 cut(s)	467, 951
SinI	GGWCC	1 cut(s)	265
SmiMI	CAYNNNNRTG	2 cut(s)	1338, 1531
SmlI	CTYRAG	1 cut(s)	411
SmoI	CTYRAG	1 cut(s)	411
Sse9I	AATT	5 cut(s)	548, 941, 964, 1209, 1395
SsiI	CCGC	5 cut(s)	724, 1246, 1517, 1577, 1849
SspI	AATATT	1 cut(s)	694
SspMI	CTAG	2 cut(s)	300, 731
StyD4I	CCNGG	8 cut(s)	36, 125, 281, 1325, 1609, 1614, 1639, 1734
StyI	CCWWGG	3 cut(s)	220, 730, 1159
TaaI	ACNGT	4 cut(s)	217, 245, 577, 1390
TaiI	ACGT	3 cut(s)	240, 314, 1406
TaqI	TCGA	1 cut(s)	1912
TaqII	GACCGA	3 cut(s)	153, 200, 1637
TasI	AATT	5 cut(s)	548, 941, 964, 1209, 1395
TatI	WGTACW	2 cut(s)	1511, 1896
TfiI	GAWTC	3 cut(s)	658, 1301, 1644
Tru1I	TTAA	6 cut(s)	426, 1059, 1317, 1371, 1394, 1658
Tru9I	TTAA	6 cut(s)	426, 1059, 1317, 1371, 1394, 1658
TscAI	CASTG	4 cut(s)	471, 718, 1186, 1239
TseFI	GTSAC	4 cut(s)	134, 274, 709, 1729
TseI	GCWGC	6 cut(s)	85, 147, 1109, 1703, 1805, 1817
Tsp45I	GTSAC	4 cut(s)	134, 274, 709, 1729
TspDTI	ATGAA	8 cut(s)	522, 622, 934, 954, 1350, 1356, 1472, 1845
TspGWI	ACGGA	2 cut(s)	437, 1667
TspRI	CASTG	4 cut(s)	471, 718, 1186, 1239
Tth111I	GACNNNGTC	1 cut(s)	187
VpaK11BI	GGWCC	1 cut(s)	265
VspI	ATTAAT	1 cut(s)	1394
XapI	RAATTY	2 cut(s)	941, 1209
XceI	RCATGY	2 cut(s)	542, 1696
XcmI	CCANNNNNNNNNTGG	1 cut(s)	380
XmaJI	CCTAGG	1 cut(s)	730
XmiI	GTMKAC	1 cut(s)	766
XmnI	GAANNNNTTC	3 cut(s)	552, 941, 1789
XspI	CTAG	2 cut(s)	300, 731
Zsp2I	ATGCAT	1 cut(s)	1630

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.