Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001069

Physical Location & Seq

Genomic Coordinates Reverse (-)

26413544 .. 26419493

Gene Structure 5950 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g33370.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGGGGAGGCAACTGAATCTGCTGCTCATGCAAATGTTCTTTATTCTGGGTGTTTTGCCATTTCATGGATCTGCAGACCATGCTGTCTCTCCTAATTCCCCTCTCAGCTTTGGCTGGAAAGAATACACAACTTTGGTTGCTCCACCTCCCGGAAAAGTATCTCACCACCTATTGCCAACAAGGCACGCCCCAGCTAAAGCCACCCATCCTCATCATTCTGTAAGGCCTTCAAGTATTGCACTTCCACCTTCTATACCATCTTCTGGAGGTCCTGCAAAGAAATGGGTGCATGCTCCTGAACATTCCCCCTCAAGCTCACGTCACAAAAATCATCTTACAAACAAAAAGTTCCACAGCTCTGCTCCAGAACCAACCTACTCAATTCATTCCCACACTAACAGTCGACAAGGCCCTTCAGTCTCCCCATTTCGATCTCCTTCGCCTTCTTCAAAGAGCTGGACTGCACCTGCACCTGCACCAACTACTTCTGAAGGCCACTATAATATGCCCATCTTTCCACCTGCAATTTCCCCCATAGATTCTTCCTTGAAGAGGACGAAGTCCCCACCACCGTCACCGATTTTGGCACTACCACCTCCACCTCCCAATGAAGATTGTTCATCAATTACATGCACGGATCCATTAACATATACACCTCCAGGCTCAACATGTGGTTGTGTGCAGCCAATCGAAGTTAAACTCCAACTAGATGTTGCAATATACACATTCTTCCCTTCAGTTTCAAAGCTGGCTGAGGAAATTGCAGACAGTGTTGCACTAAACCATAGCCAAGTTCGCATAATGGGAGCTGATGCATCTAGTCAGCAGTTAGAGAAAACTCGCGTCCTTGTTAACTTGGTACCTACAGGACTAACATTTGATGGCACTACTGCATTTCAAATCTATGAAAAATTCTGGCGCAGACAAATTTCCATAAATGCTTCTCTCTTTGGTGATTATGAAGTATTAAATGTTCACTATCCAGGTCTTCCACCTTCTCCCCCTTCAGCACCTTCAGCCATTTCTACTATAGATGATGGGCCATACACGGGCCAAGACAACAATGGAAGGGTAATAAAACCTATAGGAGTTGATGTGCCAAGAAAAAGAAATGATGGAATCAAAGGAAGTATGATTGCTGTTATTGTTCTATCAGCTTTCACTGCCTTTCTTCTATGTGTGGGGTTTGTCTGGCTTTTGTTGCTGAAATGTCGATCTCACTCCCATAAGCCAGAACAATATCCACAACCTTTGACATCATCCCCTTCAAAATTCACAGGGCCTCCCAGGTCAGTGATGTTTGGAAGCACACATGGTTCTGGATCAATGTCTCTTAGTTCTGGCACAATAAACTATACTGGCTCTGCGAAGACCTTTACTCTGCATGACATGGAGAGAGCAACTAATAATTTTGATGCTTCAAGAATACTTGGAGAAGGTGGTTTTGGGCTTGTTTACAGTGCTACTTTGGATGATGGACGAGAAGTGGCCGTGAAGGTTCTAAAACGAGATGATCATCATGGTGGCCGCGAATTCTTAGCAGAAGTTGAGATGCTCGGTCGTCTTCACCACAGAAATTTGGTCAAATTAATTGGTATCTGCACAGAAGGCCATACTCGTTGCCTTGTGTACGAACTGGTTCCAAATGGAAGTGTTGAATCCCATTTACATGGTGTTGACAAGGAAACTGATCCCCTTGATTGGGACGCCAGAATAAAGATTGCCCTTGGTGCAGCTCGGGGCTTAGCTTATCTCCATGAGGACTCAAATCCTCGAGTTATACATCGCGACTTTAAGGCCAGCAACATCTTACTGGAATATGATTTTACACCCAAGGTTTCAGATTTTGGACTGGCTAGAGCGGCCCTGGAGGAGGGAAAAAGACATATTTCAACGCATGTTATGGGAACTTTTGGTTACTTGGCTCCAGAGTATGCAATGACAGGTCATCTTCTAGTGAAAAGTGATGTTTACAGCTATGGTGTAGTCCTTCTTGAACTCTTGACAGGAAGAAAGCCGGTGGATCTATCACAACCTGCAGGCCAAGAAAACCTCGTTGCTTGGGCTCGTCCCCTTCTCACCAGTAAAGAGGGTCTAGAATCAATAATAGACCCTGTGTTGAAGTCTGATATGATCACATGGGACAGTGTAACCAAGGTAGCAGCAATTGCTTCAATGTGTATCCAACCAGAAGTATCTCACCGCCCATTCATGGGCGAGGTTGTTCAGGCCTTGAAGCTCGTATGCAATGAGTTCAAAGAAACAAATGAACTTGGGGGTTCAACATGTTTTGGCCAAGACGATGATGATGATATCTGCATTCATACAGACAGTGGTAATCTTGGAGTGGCAGAATCAGAGCTTCAGATGCCTGTATTCGGGGATAGCCATGACAGTAAGATACCTTCAACGTCAGACTTGTTGAGTGCAAATGTGGGATTTGAGGTTGAGGGGCAGGAAATTGGATCTTTTAGGAGGTACTCTAGTTCAGGGCCTTTGAGGACTGGTAGAAGGAGACAATTTTGGCAAAGACTAAGGAGTTTGTCTAGAGGAAGTTTGAGTGAACATGGATTGCCATCAAAACTGTGGCCAAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

866

Amino Acids

94.23

Weight (kDa)

6.98

Isoelectric Point (pI)

50.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	203 - 315	2.2e-51	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	457 - 669	1.4e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	458 - 728	2.3e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56890
fragaria_vesca	FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370
malus_domestica	MD02G1079800.v1.1 MD09G1193100.v1.1 MD15G1207500.v1.1
prunus_persica	Prupe.3G049500_v2.0.a1 Prupe.7G209300_v2.0.a1 Prupe.7G209300_v2.0.a1
pyrus_communis	pycom02g06360 pycom09g10970 pycom15g18390
rosa_chinensis	RchiOBHm_Chr2g0093411 RchiOBHm_Chr2g0143231
rosa_laevigata	RLG00000016396 RLG00000020017 RLG00000020021
rosa_multiflora	Rmu_co8519777.1_g000001 Rmu_sc0003950.1_g000003
rosa_roxburghii	Rroxscaffold_2G00102000 Rroxscaffold_2G00148130
rosa_rugosa	Rorug02G0035200 Rorug02G0035200 Rorug02G0035300 Rorug02G0035400 Rorug02G0035500 Rorug02G0035600 Rorug02G0379700
rosa_samantha	Rh2AG081600 Rh2AG432500 Rh2BG082900 Rh2BG441100 Rh2CG084900 Rh2CG418300 Rh2DG080600 Rh2DG451200
rosa_wichuraiana	Rw2G006500 Rw2G035310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	475, 481, 529
AasI	GACNNNNNNGTC	1 cut(s)	83
Acc36I	ACCTGC	4 cut(s)	475, 481, 529, 2044
Acc65I	GGTACC	1 cut(s)	859
AccB1I	GGYRCC	1 cut(s)	859
AccB7I	CCANNNNNTGG	2 cut(s)	65, 263
AccBSI	CCGCTC	1 cut(s)	1862
AccI	GTMKAC	1 cut(s)	403
AccII	CGCG	3 cut(s)	843, 1530, 1788
AciI	CCGC	3 cut(s)	1528, 1862, 2203
AclWI	GGATC	7 cut(s)	76, 632, 645, 1330, 1685, 2031, 2473
AcoI	YGGCCR	4 cut(s)	1488, 1525, 2293, 2588
AcsI	RAATTY	5 cut(s)	911, 927, 1271, 1532, 1576
AcuI	CTGAAG	6 cut(s)	399, 510, 720, 990, 999, 2348
AcyI	GRCGYC	1 cut(s)	1707
AfaI	GTAC	3 cut(s)	861, 1631, 2480
AflIII	ACRYGT	2 cut(s)	668, 2285
AjiI	CACGTC	1 cut(s)	320
AjnI	CCWGG	4 cut(s)	658, 982, 1286, 1866
AjuI	GAANNNNNNNTTGG	2 cut(s)	1428, 1460
Alw26I	GTCTC	4 cut(s)	91, 424, 1335, 2509
AlwI	GGATC	7 cut(s)	76, 632, 645, 1330, 1685, 2031, 2473
Ama87I	CYCGRG	2 cut(s)	1737, 1773
ApeKI	GCWGC	4 cut(s)	22, 682, 1733, 2162
ApoI	RAATTY	5 cut(s)	911, 927, 1271, 1532, 1576
AseI	ATTAAT	1 cut(s)	1589
Asp700I	GAANNNNTTC	1 cut(s)	1638
Asp718I	GGTACC	1 cut(s)	859
AspLEI	GCGC	1 cut(s)	921
AspS9I	GGNCC	7 cut(s)	269, 410, 1040, 1051, 1280, 1864, 2492
AsuC2I	CCSGG	1 cut(s)	150
AsuHPI	GGTGA	6 cut(s)	155, 567, 965, 1559, 2071, 2192
AvaI	CYCGRG	2 cut(s)	1737, 1773
AvaII	GGWCC	1 cut(s)	269
BaeI	ACNNNNGTAYC	2 cut(s)	2470, 2503
BalI	TGGCCA	2 cut(s)	2295, 2590
BamHI	GGATCC	1 cut(s)	637
BanI	GGYRCC	1 cut(s)	859
BanII	GRGCYC	1 cut(s)	2068
BarI	GAAGNNNNNNTAC	2 cut(s)	2389, 2421
BbsI	GAAGAC	3 cut(s)	980, 1376, 1556
BbvCI	CCTCAGC	1 cut(s)	753
BbvI	GCAGC	4 cut(s)	9, 694, 1745, 2174
BccI	CCATC	8 cut(s)	213, 265, 518, 875, 1031, 1109, 1469, 2584
BceAI	ACGGC	1 cut(s)	1475
BciT130I	CCWGG	4 cut(s)	660, 984, 1288, 1868
BciVI	GTATCC	1 cut(s)	2192
BclI	TGATCA	2 cut(s)	1513, 2133
BcnI	CCSGG	1 cut(s)	150
BcoDI	GTCTC	4 cut(s)	91, 424, 1335, 2509
BfaI	CTAG	7 cut(s)	707, 819, 1857, 1955, 2096, 2484, 2547
BfmI	CTRYAG	5 cut(s)	72, 864, 1029, 1083, 2037
BfuAI	ACCTGC	4 cut(s)	475, 481, 529, 2044
BfuI	GTATCC	1 cut(s)	2192
BglI	GCCNNNNNGGC	1 cut(s)	181
BisI	GCNGC	6 cut(s)	23, 683, 1528, 1734, 1863, 2163
BlpI	GCTNAGC	1 cut(s)	1744
BlsI	GCNGC	6 cut(s)	24, 684, 1529, 1735, 1864, 2164
Bme1390I	CCNGG	5 cut(s)	150, 660, 984, 1288, 1868
Bme18I	GGWCC	1 cut(s)	269
BmeT110I	CYCGRG	2 cut(s)	1737, 1773
BmgBI	CACGTC	1 cut(s)	320
BmgT120I	GGNCC	7 cut(s)	269, 410, 1040, 1051, 1280, 1864, 2492
BmiI	GGNNCC	4 cut(s)	639, 861, 1641, 1926
BmrFI	CCNGG	5 cut(s)	150, 660, 984, 1288, 1868
BmsI	GCATC	5 cut(s)	802, 824, 1405, 1542, 2358
BpiI	GAAGAC	3 cut(s)	980, 1376, 1556
BpmI	CTGGAG	5 cut(s)	285, 348, 642, 1889, 1911
Bpu10I	CCTNAGC	1 cut(s)	753
Bpu1102I	GCTNAGC	1 cut(s)	1744
BpuEI	CTTGAG	1 cut(s)	295
BpuMI	CCSGG	1 cut(s)	150
BsaBI	GATNNNNATC	2 cut(s)	1515, 2575
BsaHI	GRCGYC	1 cut(s)	1707
BsaJI	CCNNGG	5 cut(s)	1286, 1726, 1833, 1866, 2154
BsaXI	ACNNNNNCTCC	4 cut(s)	1080, 1110, 1267, 1297
Bse118I	RCCGGY	1 cut(s)	2017
Bse1I	ACTGG	6 cut(s)	1363, 1641, 1818, 1857, 2082, 2509
Bse3DI	GCAATG	2 cut(s)	1944, 2254
Bse8I	GATNNNNATC	2 cut(s)	1515, 2575
BseBI	CCWGG	4 cut(s)	660, 984, 1288, 1868
BseDI	CCNNGG	5 cut(s)	1286, 1726, 1833, 1866, 2154
BseGI	GGATG	3 cut(s)	205, 1259, 1477
BseJI	GATNNNNATC	2 cut(s)	1515, 2575
BseMI	GCAATG	2 cut(s)	1944, 2254
BseMII	CTCAG	2 cut(s)	118, 744
BseNI	ACTGG	6 cut(s)	1363, 1641, 1818, 1857, 2082, 2509
BseRI	GAGGAG	1 cut(s)	1886
BseXI	GCAGC	4 cut(s)	9, 694, 1745, 2174
BseYI	CCCAGC	1 cut(s)	190
BsgI	GTGCAG	6 cut(s)	447, 453, 459, 701, 1585, 1752
Bsh1236I	CGCG	3 cut(s)	843, 1530, 1788
Bsh1285I	CGRYCG	1 cut(s)	1561
BshNI	GGYRCC	1 cut(s)	859
BsiEI	CGRYCG	1 cut(s)	1561
BsiHKCI	CYCGRG	2 cut(s)	1737, 1773
BsiSI	CCGG	2 cut(s)	150, 2018
BslFI	GGGAC	4 cut(s)	547, 1718, 2055, 2156
BsmAI	GTCTC	4 cut(s)	91, 424, 1335, 2509
BsmFI	GGGAC	4 cut(s)	547, 1718, 2055, 2156
BsmI	GAATGC	1 cut(s)	2319
BsoBI	CYCGRG	2 cut(s)	1737, 1773
Bsp1286I	GDGCHC	1 cut(s)	2068
Bsp1720I	GCTNAGC	1 cut(s)	1744
Bsp68I	TCGCGA	1 cut(s)	1788
BspACI	CCGC	3 cut(s)	1528, 1862, 2203
BspCNI	CTCAG	2 cut(s)	117, 745
BspFNI	CGCG	3 cut(s)	843, 1530, 1788
BspLI	GGNNCC	4 cut(s)	639, 861, 1641, 1926
BspMAI	CTGCAG	2 cut(s)	76, 2041
BspMI	ACCTGC	4 cut(s)	475, 481, 529, 2044
BspPI	GGATC	7 cut(s)	76, 632, 645, 1330, 1685, 2031, 2473
BspT107I	GGYRCC	1 cut(s)	859
BsrBI	CCGCTC	1 cut(s)	1862
BsrDI	GCAATG	2 cut(s)	1944, 2254
BsrFI	RCCGGY	1 cut(s)	2017
BsrI	ACTGG	6 cut(s)	1363, 1641, 1818, 1857, 2082, 2509
BssAI	RCCGGY	1 cut(s)	2017
BssECI	CCNNGG	5 cut(s)	1286, 1726, 1833, 1866, 2154
BssNI	GRCGYC	1 cut(s)	1707
BssT1I	CCWWGG	3 cut(s)	1726, 1833, 2154
Bst2UI	CCWGG	4 cut(s)	660, 984, 1288, 1868
Bst4CI	ACNGT	8 cut(s)	401, 573, 770, 1460, 2147, 2333, 2396, 2586
Bst6I	CTCTTC	1 cut(s)	545
BstACI	GRCGYC	1 cut(s)	1707
BstAPI	GCANNNNNTGC	2 cut(s)	80, 2168
BstC8I	GCNNGC	5 cut(s)	186, 291, 750, 1801, 2041
BstDEI	CTNAG	6 cut(s)	104, 753, 1334, 1537, 1744, 2534
BstENI	CCTNNNNNAGG	2 cut(s)	550, 1871
BstF5I	GGATG	3 cut(s)	205, 1259, 1477
BstFNI	CGCG	3 cut(s)	843, 1530, 1788
BstHHI	GCGC	1 cut(s)	921
BstMAI	GTCTC	4 cut(s)	91, 424, 1335, 2509
BstMCI	CGRYCG	1 cut(s)	1561
BstNI	CCWGG	4 cut(s)	660, 984, 1288, 1868
BstNSI	RCATGY	5 cut(s)	293, 633, 672, 1901, 2289
BstSCI	CCNGG	5 cut(s)	148, 658, 982, 1286, 1866
BstSFI	CTRYAG	5 cut(s)	72, 864, 1029, 1083, 2037
BstUI	CGCG	3 cut(s)	843, 1530, 1788
BstV1I	GCAGC	4 cut(s)	9, 694, 1745, 2174
BstV2I	GAAGAC	3 cut(s)	980, 1376, 1556
BstX2I	RGATCY	4 cut(s)	68, 637, 2023, 2465
BstXI	CCANNNNNNTGG	1 cut(s)	1670
BstYI	RGATCY	4 cut(s)	68, 637, 2023, 2465
BsuI	GTATCC	1 cut(s)	2192
BtgZI	GCGATG	1 cut(s)	1769
BtrI	CACGTC	1 cut(s)	320
BtsCI	GGATG	3 cut(s)	205, 1259, 1477
BtsI	GCAGTG	1 cut(s)	1161
BtsIMutI	CAGTG	6 cut(s)	775, 1161, 1299, 1465, 2152, 2338
BtuMI	TCGCGA	1 cut(s)	1788
BveI	ACCTGC	4 cut(s)	475, 481, 529, 2044
Cac8I	GCNNGC	5 cut(s)	186, 291, 750, 1801, 2041
CfoI	GCGC	1 cut(s)	921
Cfr10I	RCCGGY	1 cut(s)	2017
Cfr13I	GGNCC	7 cut(s)	269, 410, 1040, 1051, 1280, 1864, 2492
CseI	GACGC	2 cut(s)	832, 1715
Csp6I	GTAC	3 cut(s)	860, 1630, 2479
CspCI	CAANNNNNGTGG	2 cut(s)	1233, 1268
CviQI	GTAC	3 cut(s)	860, 1630, 2479
DdeI	CTNAG	6 cut(s)	104, 753, 1334, 1537, 1744, 2534
DrdI	GACNNNNNNGTC	1 cut(s)	83
DseDI	GACNNNNNNGTC	1 cut(s)	83
EaeI	YGGCCR	4 cut(s)	1488, 1525, 2293, 2588
Eam1104I	CTCTTC	1 cut(s)	545
EarI	CTCTTC	1 cut(s)	545
Eco130I	CCWWGG	3 cut(s)	1726, 1833, 2154
Eco147I	AGGCCT	2 cut(s)	226, 2231
Eco24I	GRGCYC	1 cut(s)	2068
Eco32I	GATATC	1 cut(s)	2314
Eco47I	GGWCC	1 cut(s)	269
Eco57I	CTGAAG	6 cut(s)	399, 510, 720, 990, 999, 2348
Eco88I	CYCGRG	2 cut(s)	1737, 1773
EcoNI	CCTNNNNNAGG	2 cut(s)	550, 1871
EcoO109I	RGGNCCY	4 cut(s)	269, 410, 1280, 2492
EcoRI	GAATTC	1 cut(s)	1532
EcoRII	CCWGG	4 cut(s)	658, 982, 1286, 1866
EcoRV	GATATC	1 cut(s)	2314
EcoT14I	CCWWGG	3 cut(s)	1726, 1833, 2154
EcoT22I	ATGCAT	1 cut(s)	817
EcoT38I	GRGCYC	1 cut(s)	2068
ErhI	CCWWGG	3 cut(s)	1726, 1833, 2154
FaqI	GGGAC	4 cut(s)	547, 1718, 2055, 2156
FbaI	TGATCA	2 cut(s)	1513, 2133
FblI	GTMKAC	1 cut(s)	403
Fnu4HI	GCNGC	6 cut(s)	23, 683, 1528, 1734, 1863, 2163
FokI	GGATG	3 cut(s)	192, 1246, 1484
FriOI	GRGCYC	1 cut(s)	2068
Fsp4HI	GCNGC	6 cut(s)	23, 683, 1528, 1734, 1863, 2163
FspBI	CTAG	7 cut(s)	707, 819, 1857, 1955, 2096, 2484, 2547
GlaI	GCGC	1 cut(s)	920
GluI	GCNGC	6 cut(s)	23, 683, 1528, 1734, 1863, 2163
GsaI	CCCAGC	1 cut(s)	194
GsuI	CTGGAG	5 cut(s)	285, 348, 642, 1889, 1911
HapII	CCGG	2 cut(s)	150, 2018
HgaI	GACGC	2 cut(s)	832, 1715
HhaI	GCGC	1 cut(s)	921
Hin1I	GRCGYC	1 cut(s)	1707
Hin6I	GCGC	1 cut(s)	919
HinP1I	GCGC	1 cut(s)	919
HincII	GTYRAC	3 cut(s)	404, 853, 1678
HindII	GTYRAC	3 cut(s)	404, 853, 1678
HinfI	GANTC	7 cut(s)	16, 539, 1119, 1658, 1763, 2099, 2354
HpaI	GTTAAC	1 cut(s)	853
HpaII	CCGG	2 cut(s)	150, 2018
HphI	GGTGA	6 cut(s)	155, 567, 965, 1559, 2071, 2192
Hpy166II	GTNNAC	8 cut(s)	404, 853, 976, 1456, 1630, 1678, 1972, 2564
Hpy188I	TCNGA	6 cut(s)	490, 1843, 2128, 2359, 2367, 2416
Hpy8I	GTNNAC	8 cut(s)	404, 853, 976, 1456, 1630, 1678, 1972, 2564
HpyCH4III	ACNGT	8 cut(s)	401, 573, 770, 1460, 2147, 2333, 2396, 2586
HpyCH4IV	ACGT	2 cut(s)	319, 2411
HpyF3I	CTNAG	6 cut(s)	104, 753, 1334, 1537, 1744, 2534
HpySE526I	ACGT	2 cut(s)	319, 2411
Hsp92I	GRCGYC	1 cut(s)	1707
HspAI	GCGC	1 cut(s)	919
KpnI	GGTACC	1 cut(s)	863
Ksp22I	TGATCA	2 cut(s)	1513, 2133
KspAI	GTTAAC	1 cut(s)	853
LmnI	GCTCC	5 cut(s)	145, 298, 367, 806, 1930
Lsp1109I	GCAGC	4 cut(s)	9, 694, 1745, 2174
LweI	GCATC	5 cut(s)	802, 824, 1405, 1542, 2358
MaeI	CTAG	7 cut(s)	707, 819, 1857, 1955, 2096, 2484, 2547
MaeII	ACGT	2 cut(s)	319, 2411
MaeIII	GTNAC	4 cut(s)	320, 573, 1916, 2149
MbiI	CCGCTC	1 cut(s)	1862
MfeI	CAATTG	1 cut(s)	2166
MflI	RGATCY	4 cut(s)	68, 637, 2023, 2465
MhlI	GDGCHC	1 cut(s)	2068
MlsI	TGGCCA	2 cut(s)	2295, 2590
MluNI	TGGCCA	2 cut(s)	2295, 2590
MlyI	GAGTC	1 cut(s)	1757
MmeI	TCCRAC	2 cut(s)	727, 2209
Mox20I	TGGCCA	2 cut(s)	2295, 2590
Mph1103I	ATGCAT	1 cut(s)	817
MroXI	GAANNNNTTC	1 cut(s)	1638
MscI	TGGCCA	2 cut(s)	2295, 2590
MseI	TTAA	6 cut(s)	644, 696, 852, 968, 1589, 1794
MslI	CAYNNNNRTG	3 cut(s)	32, 1521, 1668
Msp20I	TGGCCA	2 cut(s)	2295, 2590
MspI	CCGG	2 cut(s)	150, 2018
MspR9I	CCNGG	5 cut(s)	150, 660, 984, 1288, 1868
MunI	CAATTG	1 cut(s)	2166
Mva1269I	GAATGC	1 cut(s)	2319
MvaI	CCWGG	4 cut(s)	660, 984, 1288, 1868
MvnI	CGCG	3 cut(s)	843, 1530, 1788
NciI	CCSGG	1 cut(s)	150
NlaIV	GGNNCC	4 cut(s)	639, 861, 1641, 1926
NmuCI	GTSAC	2 cut(s)	320, 573
NruI	TCGCGA	1 cut(s)	1788
NsiI	ATGCAT	1 cut(s)	817
NspI	RCATGY	5 cut(s)	293, 633, 672, 1901, 2289
PaeI	GCATGC	1 cut(s)	293
PaeR7I	CTCGAG	1 cut(s)	1773
PaqCI	CACCTGC	3 cut(s)	475, 481, 529
PceI	AGGCCT	2 cut(s)	226, 2231
PciI	ACATGT	2 cut(s)	668, 2285
PctI	GAATGC	1 cut(s)	2319
PdmI	GAANNNNTTC	1 cut(s)	1638
PfeI	GAWTC	6 cut(s)	16, 539, 1119, 1658, 2099, 2354
PflFI	GACNNNGTC	1 cut(s)	559
PflMI	CCANNNNNTGG	2 cut(s)	65, 263
PfoI	TCCNGGA	1 cut(s)	148
PkrI	GCNGC	6 cut(s)	24, 684, 1529, 1735, 1864, 2164
PleI	GAGTC	1 cut(s)	1757
PpsI	GAGTC	1 cut(s)	1757
PpuMI	RGGWCCY	1 cut(s)	269
PscI	ACATGT	2 cut(s)	668, 2285
PshBI	ATTAAT	1 cut(s)	1589
Psp5II	RGGWCCY	1 cut(s)	269
Psp6I	CCWGG	4 cut(s)	658, 982, 1286, 1866
PspFI	CCCAGC	1 cut(s)	190
PspGI	CCWGG	4 cut(s)	658, 982, 1286, 1866
PspN4I	GGNNCC	4 cut(s)	639, 861, 1641, 1926
PspPI	GGNCC	7 cut(s)	269, 410, 1040, 1051, 1280, 1864, 2492
PspPPI	RGGWCCY	1 cut(s)	269
PspXI	VCTCGAGB	1 cut(s)	1773
PsrI	GAACNNNNNNTAC	2 cut(s)	957, 989
PstI	CTGCAG	2 cut(s)	76, 2041
PsuI	RGATCY	4 cut(s)	68, 637, 2023, 2465
PsyI	GACNNNGTC	1 cut(s)	559
RruI	TCGCGA	1 cut(s)	1788
RsaI	GTAC	3 cut(s)	861, 1631, 2480
RsaNI	GTAC	3 cut(s)	860, 1630, 2479
RseI	CAYNNNNRTG	3 cut(s)	32, 1521, 1668
SalI	GTCGAC	1 cut(s)	402
SaqAI	TTAA	6 cut(s)	644, 696, 852, 968, 1589, 1794
SatI	GCNGC	6 cut(s)	23, 683, 1528, 1734, 1863, 2163
Sau96I	GGNCC	7 cut(s)	269, 410, 1040, 1051, 1280, 1864, 2492
SbfI	CCTGCAGG	1 cut(s)	2041
SchI	GAGTC	1 cut(s)	1757
ScrFI	CCNGG	5 cut(s)	150, 660, 984, 1288, 1868
SdaI	CCTGCAGG	1 cut(s)	2041
SduI	GDGCHC	1 cut(s)	2068
SfaNI	GCATC	5 cut(s)	802, 824, 1405, 1542, 2358
SfcI	CTRYAG	5 cut(s)	72, 864, 1029, 1083, 2037
Sfr274I	CTCGAG	1 cut(s)	1773
SinI	GGWCC	1 cut(s)	269
SlaI	CTCGAG	1 cut(s)	1773
SmiMI	CAYNNNNRTG	3 cut(s)	32, 1521, 1668
SmlI	CTYRAG	2 cut(s)	310, 1773
SmoI	CTYRAG	2 cut(s)	310, 1773
SphI	GCATGC	1 cut(s)	293
Sse8387I	CCTGCAGG	1 cut(s)	2041
SseBI	AGGCCT	2 cut(s)	226, 2231
SsiI	CCGC	3 cut(s)	1528, 1862, 2203
SspMI	CTAG	7 cut(s)	707, 819, 1857, 1955, 2096, 2484, 2547
StuI	AGGCCT	2 cut(s)	226, 2231
StyD4I	CCNGG	5 cut(s)	148, 658, 982, 1286, 1866
StyI	CCWWGG	3 cut(s)	1726, 1833, 2154
TaaI	ACNGT	8 cut(s)	401, 573, 770, 1460, 2147, 2333, 2396, 2586
TaiI	ACGT	2 cut(s)	322, 2414
TaqI	TCGA	5 cut(s)	403, 430, 690, 1213, 1774
TaqII	GACCGA	1 cut(s)	1547
TauI	GCSGC	2 cut(s)	1530, 1865
TfiI	GAWTC	6 cut(s)	16, 539, 1119, 1658, 2099, 2354
Tru1I	TTAA	6 cut(s)	644, 696, 852, 968, 1589, 1794
Tru9I	TTAA	6 cut(s)	644, 696, 852, 968, 1589, 1794
TscAI	CASTG	6 cut(s)	775, 1168, 1299, 1465, 2152, 2338
TseFI	GTSAC	2 cut(s)	320, 573
TseI	GCWGC	4 cut(s)	22, 682, 1733, 2162
Tsp45I	GTSAC	2 cut(s)	320, 573
TspDTI	ATGAA	9 cut(s)	53, 374, 609, 624, 921, 975, 2200, 2283, 2312
TspGWI	ACGGA	1 cut(s)	650
TspRI	CASTG	6 cut(s)	775, 1168, 1299, 1465, 2152, 2338
Tth111I	GACNNNGTC	1 cut(s)	559
Van91I	CCANNNNNTGG	2 cut(s)	65, 263
VpaK11BI	GGWCC	1 cut(s)	269
VspI	ATTAAT	1 cut(s)	1589
XagI	CCTNNNNNAGG	2 cut(s)	550, 1871
XapI	RAATTY	5 cut(s)	911, 927, 1271, 1532, 1576
XbaI	TCTAGA	2 cut(s)	2095, 2546
XceI	RCATGY	5 cut(s)	293, 633, 672, 1901, 2289
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1061, 2583
XhoI	CTCGAG	1 cut(s)	1773
XmiI	GTMKAC	1 cut(s)	403
XmnI	GAANNNNTTC	1 cut(s)	1638
XspI	CTAG	7 cut(s)	707, 819, 1857, 1955, 2096, 2484, 2547
Zsp2I	ATGCAT	1 cut(s)	817

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.