Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001069

Physical Location & Seq

Genomic Coordinates Forward (+)

17260611 .. 17265617

Gene Structure 5007 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1193100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2457 bp

ATGGGCATGCAACTGCATCTTCTGCTTCTGCAAATGTGTTTGATTGCTGGTGCTCTACCATTTCATGGATCTGCAGCCACCCATCCTCGCGATGCCTTAAGGCCTTCAAGTATTGCAGTTCCACCTTCTCTGTCACCCTCCGGAGGTCCTGCAACGAAATGGGTGCATGGTCCCGTATCTTCTCCTTTGGTCTCACATCAAAAACATCACCACAGTAGAAAAAAGTTCCACAGCTCTGCTCCAGGACCAACTTATCCGTTTCATGCAAACACTTACAATGGACAAGGCCCTTCAGTCTCACCTGTTCAGTCACTCTCTCCGTCAAGGAGCTGGGTTGCACCTGCACCATCACCGACAACTCTCACAAGCCACCATAACATGCCCATCTTCCCCCCTGCAGCTTCTCCCGTCGATTCTTCCTTCAAGACGACAAGGGCTCCACAACCATCACCAATTTTAGCACTACCACCTCCACCTCCCAACGAAGATTGTTCATCAATCACATGTACAGAACCGTTGACTTATACACCTCCTGGATCAAGCTGTGGTTGTGTGAGGCCAATTCAAGTTAAACTCCAACTAGATGTTGCAATATACACATTCTTCCCTTTAGTTACAGAGCTGGCTGAGGAAATTGCAGCCAGTGTGGCAATAAACCGTAGCCAAGTTCGAATTATGGGAGCAGATGCAGCTAGTCAGCAGCTAGAGAAAAGCGCTGTCCTCATTAACTTGGTACCCACAGGAATAACGTTTGATGGCACTACTGCATTCCAAATCTACGAAAAATTCTGGCGCAGACAGGTTTCCATAAAGCCTTCGCTCTTTGGAGATTATGAAGTCTTGTATGTTCACTATCCAGGTCTCCCACCTTCTCCCCCTTCAGCACCGTCAACCATTTCTACTATAGATGATGGGCCATACATCGGAAATGACAACCATGGAAGGGCAATAAAACCTTTAGGTGTTGATGTGCCGAGGAGAAAAAAAGGTGGACTTGGAAGAAGTATGATTGCTGTGATTGCTCTATCATCCTTCACAGCCTTTCTTTTATGTGTGGGAGGTATTTGGATTTTGTTGTTGAAATGTGGACCTTGTGCCCGACAACAGGAAAATTTTGCACAAACTTTATCATCCTCTCCTACAAAACTCTCAGGGCCTCCAAGGTCAGCGATGTTTGGAAGCATGCGTGGTTCTGAATCAACATCCTTGAGCTCCGGCACCATGAACTATACTGGATCTGCTAAAACATTCACTTTGAATGACATAGAGAGAGCAACTAATACTTTTGATGCCTCGAGAATACTGGGAGAAGGTGGGTTTGGGCTTGTTTACGGTGGAATTTTAGATGATGGAAGAGAGGTGGCTGTCAAGGTTCTCAAGAGAGATGATCATCATGGTAGCCGGGAATTCTTGGCAGAAGTTGAGATGCTTGGCCGTCTACACCATAGAAATTTGGTTAAATTGATTGGTATCTGCATAGAGGGCCATGCACGTTGCCTGGTATATGAACTCGTTCCAAATGGGAGTGTTGAATCCCATTTACACGGCATTGACAAGGAAAATGATCCGCTTGATTGGGATGCCAGAATAAAGATTGCCCTTGGTGCAGCTCGGGGCTTAGCTTATCTCCACGAAGACTCAAACCCTCGTGTTATACATCGTGACTTCAAGGCCAGCAATATCTTGTTGGAATACGATTGTACACCCAAGGTTTCAGATTTTGGATTGGCTAGAGCAGCATTAGAGGAGGGGAAGAGACATATTTCAACACATGTCATGGGAACTTTCGGGTACTTGGCTCCAGAGTATGCAATGACCGGCCATTTGTTAGTGAAAAGCGACGTTTACAGCTATGGTGTAGTCCTTCTTGAGCTTCTATCAGGGAGAAAGCCAGTGGACCTGTCACAACCTCCAGGCCAAGAAAACCTTGTTGCTTGGGCGCGGCCTCTTCTCACAAGCAAGGAGGGGATAGAAACAGTTATAGACCCAGCCTTAAAGCCATCCGATATCTCATGGGATAGTGTGACCAAGGTGGCAGCAATTGCTTCAATGTGCATACAACCAGAAGTATCTCACCGCCCCTTCATGGGTGAGGTTGTGCAGGCCTTAAAGCTAGTATGTAATGAGTTCAACGAAACACAAGAACCGGGGGGTTCAATAGGTTGTGGTCAAGATGATCTTTCCCTTCATATAGATAGCGGTAACTTGGAAGTATCACGATCACTGCATGAGCAGGAGCTGCCGGCTGTATTTGGTGCTGGCCACGACACCAAGATACCATCAACATCGGATTTGCTAAGTGCATCTGTGGAGGGACTCAAGGTTGAAGGGCAGGAATTCGGGTCTTTCAGGCGGTACTCTAGTTCGGGACCTCTGAGGACAGGGAGAAAAGTGAAGTTTTGGCAGAGACTACGAAGTTTGTCTAGAGGCAGCATGAGTGAACATGGGTTATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

819

Amino Acids

88.26

Weight (kDa)

7.19

Isoelectric Point (pI)

48.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	170 - 289	9.2e-56	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	431 - 640	9.4e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	432 - 703	2.6e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0001069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56890
fragaria_vesca	FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370
malus_domestica	MD02G1079800.v1.1 MD09G1193100.v1.1 MD15G1207500.v1.1
prunus_persica	Prupe.3G049500_v2.0.a1 Prupe.7G209300_v2.0.a1 Prupe.7G209300_v2.0.a1
pyrus_communis	pycom02g06360 pycom09g10970 pycom15g18390
rosa_chinensis	RchiOBHm_Chr2g0093411 RchiOBHm_Chr2g0143231
rosa_laevigata	RLG00000016396 RLG00000020017 RLG00000020021
rosa_multiflora	Rmu_co8519777.1_g000001 Rmu_sc0003950.1_g000003
rosa_roxburghii	Rroxscaffold_2G00102000 Rroxscaffold_2G00148130
rosa_rugosa	Rorug02G0035200 Rorug02G0035200 Rorug02G0035300 Rorug02G0035400 Rorug02G0035500 Rorug02G0035600 Rorug02G0379700
rosa_samantha	Rh2AG081600 Rh2AG432500 Rh2BG082900 Rh2BG441100 Rh2CG084900 Rh2CG418300 Rh2DG080600 Rh2DG451200
rosa_wichuraiana	Rw2G006500 Rw2G035310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	349
Acc36I	ACCTGC	1 cut(s)	349
Acc65I	GGTACC	1 cut(s)	733
AccB1I	GGYRCC	2 cut(s)	733, 1217
AccB7I	CCANNNNNTGG	1 cut(s)	65
AccI	GTMKAC	1 cut(s)	1438
AccII	CGCG	2 cut(s)	90, 1942
AccIII	TCCGGA	1 cut(s)	140
AciI	CCGC	5 cut(s)	1568, 1942, 2077, 2199, 2353
AclI	AACGTT	1 cut(s)	749
AclWI	GGATC	4 cut(s)	76, 544, 1243, 1559
AcoI	YGGCCR	3 cut(s)	1432, 1819, 2260
AcsI	RAATTY	6 cut(s)	785, 1111, 1338, 1406, 1450, 2336
AcuI	CTGAAG	2 cut(s)	276, 864
AfaI	GTAC	5 cut(s)	508, 735, 1702, 1793, 2357
AfeI	AGCGCT	1 cut(s)	715
AfiI	CCNNNNNNNGG	7 cut(s)	65, 143, 923, 943, 1105, 2086, 2087
AflII	CTTAAG	1 cut(s)	97
AflIII	ACRYGT	2 cut(s)	503, 1771
AjnI	CCWGG	5 cut(s)	241, 532, 856, 1497, 1912
AjuI	GAANNNNNNNTTGG	2 cut(s)	1302, 1334
Alw21I	GWGCWC	2 cut(s)	55, 1214
Alw26I	GTCTC	5 cut(s)	196, 301, 866, 1750, 2401
AlwI	GGATC	4 cut(s)	76, 544, 1243, 1559
AlwNI	CAGNNNCTG	1 cut(s)	2239
Ama87I	CYCGRG	2 cut(s)	1294, 1611
Aor13HI	TCCGGA	1 cut(s)	140
Aor51HI	AGCGCT	1 cut(s)	715
ApoI	RAATTY	6 cut(s)	785, 1111, 1338, 1406, 1450, 2336
ArsI	GACNNNNNNTTYG	2 cut(s)	1807, 1839
Asp700I	GAANNNNTTC	1 cut(s)	1512
Asp718I	GGTACC	1 cut(s)	733
AspLEI	GCGC	3 cut(s)	716, 795, 1942
AsuC2I	CCSGG	2 cut(s)	1403, 2148
AsuHPI	GGTGA	7 cut(s)	126, 200, 291, 342, 441, 2066, 2102
AsuII	TTCGAA	1 cut(s)	670
AvaI	CYCGRG	2 cut(s)	1294, 1611
AvaII	GGWCC	6 cut(s)	146, 170, 245, 1088, 1897, 2369
BaeGI	GKGCMC	1 cut(s)	1099
BaeI	ACNNNNGTAYC	2 cut(s)	2347, 2380
BalI	TGGCCA	1 cut(s)	2262
BanI	GGYRCC	2 cut(s)	733, 1217
BanII	GRGCYC	2 cut(s)	439, 1214
BauI	CACGAG	1 cut(s)	1647
BbsI	GAAGAC	1 cut(s)	1641
Bbv12I	GWGCWC	2 cut(s)	55, 1214
BbvCI	CCTCAGC	1 cut(s)	627
BccI	CCATC	9 cut(s)	90, 355, 392, 454, 749, 905, 1343, 2008, 2287
BceAI	ACGGC	2 cut(s)	1419, 1561
BcgI	CGANNNNNNTGC	2 cut(s)	145, 179
BciT130I	CCWGG	5 cut(s)	243, 534, 858, 1499, 1914
BclI	TGATCA	1 cut(s)	1387
BcnI	CCSGG	2 cut(s)	1403, 2148
BcoDI	GTCTC	5 cut(s)	196, 301, 866, 1750, 2401
BfaI	CTAG	7 cut(s)	581, 693, 704, 1731, 2114, 2361, 2424
BfmI	CTRYAG	3 cut(s)	72, 396, 903
BfoI	RGCGCY	1 cut(s)	717
BfrI	CTTAAG	1 cut(s)	97
BfuAI	ACCTGC	1 cut(s)	349
BglI	GCCNNNNNGGC	1 cut(s)	647
BlpI	GCTNAGC	1 cut(s)	1618
Bme1390I	CCNGG	7 cut(s)	243, 534, 858, 1403, 1499, 1914, 2148
Bme18I	GGWCC	6 cut(s)	146, 170, 245, 1088, 1897, 2369
BmeT110I	CYCGRG	2 cut(s)	1294, 1611
BmiI	GGNNCC	6 cut(s)	172, 438, 735, 1219, 1800, 2370
BmrFI	CCNGG	7 cut(s)	243, 534, 858, 1403, 1499, 1914, 2148
BmrI	ACTGGG	1 cut(s)	1313
BmsI	GCATC	7 cut(s)	25, 82, 676, 1279, 1416, 1570, 2312
BmuI	ACTGGG	1 cut(s)	1313
BpiI	GAAGAC	1 cut(s)	1641
BpmI	CTGGAG	3 cut(s)	225, 1785, 1896
Bpu10I	CCTNAGC	1 cut(s)	627
Bpu1102I	GCTNAGC	1 cut(s)	1618
Bpu14I	TTCGAA	1 cut(s)	670
BpuEI	CTTGAG	4 cut(s)	1228, 1361, 1889, 2303
BpuMI	CCSGG	2 cut(s)	1403, 2148
BsaBI	GATNNNNATC	2 cut(s)	1389, 1469
BsaI	GGTCTC	2 cut(s)	196, 866
BsaJI	CCNNGG	7 cut(s)	937, 974, 1160, 1600, 1707, 2028, 2147
BsaWI	WCCGGW	1 cut(s)	140
BsaXI	ACNNNNNCTCC	2 cut(s)	421, 451
Bsc4I	CCNNNNNNNGG	7 cut(s)	65, 143, 923, 943, 1105, 2086, 2087
Bse118I	RCCGGY	2 cut(s)	1817, 2242
Bse1I	ACTGG	4 cut(s)	642, 1237, 1308, 1892
Bse3DI	GCAATG	1 cut(s)	1818
Bse8I	GATNNNNATC	2 cut(s)	1389, 1469
BseAI	TCCGGA	1 cut(s)	140
BseBI	CCWGG	5 cut(s)	243, 534, 858, 1499, 1914
BseDI	CCNNGG	7 cut(s)	937, 974, 1160, 1600, 1707, 2028, 2147
BseGI	GGATG	6 cut(s)	82, 1028, 1130, 1202, 1585, 2000
BseJI	GATNNNNATC	2 cut(s)	1389, 1469
BseLI	CCNNNNNNNGG	7 cut(s)	65, 143, 923, 943, 1105, 2086, 2087
BseMI	GCAATG	1 cut(s)	1818
BseMII	CTCAG	3 cut(s)	618, 1164, 2366
BseNI	ACTGG	4 cut(s)	642, 1237, 1308, 1892
BseRI	GAGGAG	2 cut(s)	991, 1760
BseSI	GKGCMC	1 cut(s)	1099
BseYI	CCCAGC	2 cut(s)	330, 1987
BsgI	GTGCAG	3 cut(s)	327, 1626, 2120
Bsh1236I	CGCG	2 cut(s)	90, 1942
BshNI	GGYRCC	2 cut(s)	733, 1217
BsiHKAI	GWGCWC	2 cut(s)	55, 1214
BsiHKCI	CYCGRG	2 cut(s)	1294, 1611
BsiSI	CCGG	6 cut(s)	141, 1215, 1402, 1818, 2147, 2243
BslFI	GGGAC	3 cut(s)	156, 2328, 2382
BslI	CCNNNNNNNGG	7 cut(s)	65, 143, 923, 943, 1105, 2086, 2087
BsmAI	GTCTC	5 cut(s)	196, 301, 866, 1750, 2401
BsmFI	GGGAC	3 cut(s)	156, 2328, 2382
BsmI	GAATGC	1 cut(s)	767
Bso31I	GGTCTC	2 cut(s)	196, 866
BsoBI	CYCGRG	2 cut(s)	1294, 1611
Bsp119I	TTCGAA	1 cut(s)	670
Bsp1286I	GDGCHC	4 cut(s)	55, 439, 1099, 1214
Bsp13I	TCCGGA	1 cut(s)	140
Bsp1407I	TGTACA	2 cut(s)	506, 1700
Bsp143I	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
Bsp1720I	GCTNAGC	1 cut(s)	1618
Bsp19I	CCATGG	1 cut(s)	937
Bsp68I	TCGCGA	1 cut(s)	90
BspACI	CCGC	5 cut(s)	1568, 1942, 2077, 2199, 2353
BspCNI	CTCAG	3 cut(s)	619, 1163, 2367
BspEI	TCCGGA	1 cut(s)	140
BspFNI	CGCG	2 cut(s)	90, 1942
BspLI	GGNNCC	6 cut(s)	172, 438, 735, 1219, 1800, 2370
BspMAI	CTGCAG	2 cut(s)	76, 400
BspMI	ACCTGC	1 cut(s)	349
BspPI	GGATC	4 cut(s)	76, 544, 1243, 1559
BspT104I	TTCGAA	1 cut(s)	670
BspT107I	GGYRCC	2 cut(s)	733, 1217
BspTI	CTTAAG	1 cut(s)	97
BspTNI	GGTCTC	2 cut(s)	196, 866
BsrDI	GCAATG	1 cut(s)	1818
BsrFI	RCCGGY	2 cut(s)	1817, 2242
BsrGI	TGTACA	2 cut(s)	506, 1700
BsrI	ACTGG	4 cut(s)	642, 1237, 1308, 1892
BssAI	RCCGGY	2 cut(s)	1817, 2242
BssECI	CCNNGG	7 cut(s)	937, 974, 1160, 1600, 1707, 2028, 2147
BssMI	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
BssSI	CACGAG	1 cut(s)	1647
BssT1I	CCWWGG	5 cut(s)	937, 1160, 1600, 1707, 2028
Bst2BI	CACGAG	1 cut(s)	1647
Bst2UI	CCWGG	5 cut(s)	243, 534, 858, 1499, 1914
Bst4CI	ACNGT	6 cut(s)	215, 516, 659, 888, 1334, 1978
Bst6I	CTCTTC	3 cut(s)	1348, 1748, 1953
BstAFI	CTTAAG	1 cut(s)	97
BstAPI	GCANNNNNTGC	3 cut(s)	22, 2042, 2239
BstAUI	TGTACA	2 cut(s)	506, 1700
BstBI	TTCGAA	1 cut(s)	670
BstC8I	GCNNGC	7 cut(s)	8, 624, 1184, 1675, 2103, 2244, 2260
BstDEI	CTNAG	5 cut(s)	627, 1150, 1618, 2297, 2375
BstDSI	CCRYGG	1 cut(s)	937
BstF5I	GGATG	6 cut(s)	82, 1028, 1130, 1202, 1585, 2000
BstFNI	CGCG	2 cut(s)	90, 1942
BstH2I	RGCGCY	1 cut(s)	717
BstHHI	GCGC	3 cut(s)	716, 795, 1942
BstKTI	GATC	7 cut(s)	71, 539, 1238, 1390, 1567, 2179, 2222
BstMAI	GTCTC	5 cut(s)	196, 301, 866, 1750, 2401
BstMBI	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
BstMWI	GCNNNNNNNGC	9 cut(s)	22, 647, 689, 1019, 1482, 1604, 1736, 2042, 2239
BstNI	CCWGG	5 cut(s)	243, 534, 858, 1499, 1914
BstNSI	RCATGY	5 cut(s)	10, 382, 507, 1186, 1775
BstSCI	CCNGG	7 cut(s)	241, 532, 856, 1401, 1497, 1912, 2146
BstSFI	CTRYAG	3 cut(s)	72, 396, 903
BstSLI	GKGCMC	1 cut(s)	1099
BstUI	CGCG	2 cut(s)	90, 1942
BstV2I	GAAGAC	1 cut(s)	1641
BstX2I	RGATCY	2 cut(s)	68, 1235
BstYI	RGATCY	2 cut(s)	68, 1235
BtgI	CCRYGG	1 cut(s)	937
BtgZI	GCGATG	2 cut(s)	105, 1184
BtsCI	GGATG	6 cut(s)	82, 1028, 1130, 1202, 1585, 2000
BtsI	GCAGTG	1 cut(s)	2222
BtsIMutI	CAGTG	3 cut(s)	649, 1899, 2222
BtuMI	TCGCGA	1 cut(s)	90
BveI	ACCTGC	1 cut(s)	349
Cac8I	GCNNGC	7 cut(s)	8, 624, 1184, 1675, 2103, 2244, 2260
CaiI	CAGNNNCTG	1 cut(s)	2239
CfoI	GCGC	3 cut(s)	716, 795, 1942
Cfr10I	RCCGGY	2 cut(s)	1817, 2242
Csp6I	GTAC	5 cut(s)	507, 734, 1701, 1792, 2356
CviQI	GTAC	5 cut(s)	507, 734, 1701, 1792, 2356
DdeI	CTNAG	5 cut(s)	627, 1150, 1618, 2297, 2375
DpnI	GATC	7 cut(s)	70, 538, 1237, 1389, 1566, 2178, 2221
DpnII	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
EaeI	YGGCCR	3 cut(s)	1432, 1819, 2260
Eam1104I	CTCTTC	3 cut(s)	1348, 1748, 1953
EarI	CTCTTC	3 cut(s)	1348, 1748, 1953
Ecl136II	GAGCTC	1 cut(s)	1212
Eco130I	CCWWGG	5 cut(s)	937, 1160, 1600, 1707, 2028
Eco147I	AGGCCT	2 cut(s)	103, 2105
Eco24I	GRGCYC	2 cut(s)	439, 1214
Eco31I	GGTCTC	2 cut(s)	196, 866
Eco32I	GATATC	1 cut(s)	2008
Eco47I	GGWCC	6 cut(s)	146, 170, 245, 1088, 1897, 2369
Eco47III	AGCGCT	1 cut(s)	715
Eco53kI	GAGCTC	1 cut(s)	1212
Eco57I	CTGAAG	2 cut(s)	276, 864
Eco88I	CYCGRG	2 cut(s)	1294, 1611
EcoICRI	GAGCTC	1 cut(s)	1212
EcoO109I	RGGNCCY	4 cut(s)	146, 287, 1154, 2369
EcoRI	GAATTC	2 cut(s)	1406, 2336
EcoRII	CCWGG	5 cut(s)	241, 532, 856, 1497, 1912
EcoRV	GATATC	1 cut(s)	2008
EcoT14I	CCWWGG	5 cut(s)	937, 1160, 1600, 1707, 2028
EcoT38I	GRGCYC	2 cut(s)	439, 1214
ErhI	CCWWGG	5 cut(s)	937, 1160, 1600, 1707, 2028
FalI	AAGNNNNNCTT	6 cut(s)	978, 1010, 1911, 1943, 2163, 2195
FaqI	GGGAC	3 cut(s)	156, 2328, 2382
FbaI	TGATCA	1 cut(s)	1387
FblI	GTMKAC	1 cut(s)	1438
FokI	GGATG	6 cut(s)	69, 1015, 1117, 1189, 1592, 1987
FriOI	GRGCYC	2 cut(s)	439, 1214
FspBI	CTAG	7 cut(s)	581, 693, 704, 1731, 2114, 2361, 2424
GlaI	GCGC	3 cut(s)	715, 794, 1941
GsaI	CCCAGC	2 cut(s)	334, 1991
GsuI	CTGGAG	3 cut(s)	225, 1785, 1896
HaeII	RGCGCY	1 cut(s)	717
HapII	CCGG	6 cut(s)	141, 1215, 1402, 1818, 2147, 2243
HhaI	GCGC	3 cut(s)	716, 795, 1942
Hin6I	GCGC	3 cut(s)	714, 793, 1940
HinP1I	GCGC	3 cut(s)	714, 793, 1940
HincII	GTYRAC	2 cut(s)	519, 891
HindII	GTYRAC	2 cut(s)	519, 891
HinfI	GANTC	5 cut(s)	413, 1196, 1532, 1637, 2316
HpaII	CCGG	6 cut(s)	141, 1215, 1402, 1818, 2147, 2243
HphI	GGTGA	7 cut(s)	126, 200, 291, 342, 441, 2066, 2102
Hpy188I	TCNGA	6 cut(s)	926, 1195, 1717, 2005, 2290, 2376
Hpy99I	CGWCG	2 cut(s)	413, 1844
HpyCH4III	ACNGT	6 cut(s)	215, 516, 659, 888, 1334, 1978
HpyCH4IV	ACGT	3 cut(s)	749, 1492, 1842
HpyF10VI	GCNNNNNNNGC	9 cut(s)	22, 647, 689, 1019, 1482, 1604, 1736, 2042, 2239
HpyF3I	CTNAG	5 cut(s)	627, 1150, 1618, 2297, 2375
HpySE526I	ACGT	3 cut(s)	749, 1492, 1842
HspAI	GCGC	3 cut(s)	714, 793, 1940
Kpn2I	TCCGGA	1 cut(s)	140
KpnI	GGTACC	1 cut(s)	737
KroI	GCCGGC	1 cut(s)	2242
KroNI	GCCGGC	1 cut(s)	2244
Ksp22I	TGATCA	1 cut(s)	1387
Kzo9I	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
LmnI	GCTCC	7 cut(s)	244, 327, 442, 680, 1217, 1804, 2236
LweI	GCATC	7 cut(s)	25, 82, 676, 1279, 1416, 1570, 2312
MaeI	CTAG	7 cut(s)	581, 693, 704, 1731, 2114, 2361, 2424
MaeII	ACGT	3 cut(s)	749, 1492, 1842
MaeIII	GTNAC	7 cut(s)	132, 309, 613, 1661, 1902, 2023, 2201
MalI	GATC	7 cut(s)	70, 538, 1237, 1389, 1566, 2178, 2221
MboI	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
MfeI	CAATTG	1 cut(s)	2040
MflI	RGATCY	2 cut(s)	68, 1235
MhlI	GDGCHC	4 cut(s)	55, 439, 1099, 1214
MlsI	TGGCCA	1 cut(s)	2262
MluNI	TGGCCA	1 cut(s)	2262
MlyI	GAGTC	2 cut(s)	1631, 2310
MmeI	TCCRAC	2 cut(s)	601, 1668
Mox20I	TGGCCA	1 cut(s)	2262
MroI	TCCGGA	1 cut(s)	140
MroNI	GCCGGC	1 cut(s)	2242
MroXI	GAANNNNTTC	1 cut(s)	1512
MscI	TGGCCA	1 cut(s)	2262
MseI	TTAA	6 cut(s)	98, 570, 726, 1458, 1994, 2108
Msp20I	TGGCCA	1 cut(s)	2262
MspCI	CTTAAG	1 cut(s)	97
MspI	CCGG	6 cut(s)	141, 1215, 1402, 1818, 2147, 2243
MspR9I	CCNGG	7 cut(s)	243, 534, 858, 1403, 1499, 1914, 2148
MunI	CAATTG	1 cut(s)	2040
Mva1269I	GAATGC	1 cut(s)	767
MvaI	CCWGG	5 cut(s)	243, 534, 858, 1499, 1914
MvnI	CGCG	2 cut(s)	90, 1942
MwoI	GCNNNNNNNGC	9 cut(s)	22, 647, 689, 1019, 1482, 1604, 1736, 2042, 2239
NaeI	GCCGGC	1 cut(s)	2244
NciI	CCSGG	2 cut(s)	1403, 2148
NcoI	CCATGG	1 cut(s)	937
NdeII	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
NgoMIV	GCCGGC	1 cut(s)	2242
NlaIV	GGNNCC	6 cut(s)	172, 438, 735, 1219, 1800, 2370
NmeAIII	GCCGAG	1 cut(s)	999
NmuCI	GTSAC	5 cut(s)	132, 309, 1661, 1902, 2023
NruI	TCGCGA	1 cut(s)	90
NspI	RCATGY	5 cut(s)	10, 382, 507, 1186, 1775
NspV	TTCGAA	1 cut(s)	670
PaeI	GCATGC	2 cut(s)	10, 1186
PaeR7I	CTCGAG	1 cut(s)	1294
PaqCI	CACCTGC	1 cut(s)	349
PceI	AGGCCT	2 cut(s)	103, 2105
PciI	ACATGT	2 cut(s)	503, 1771
PctI	GAATGC	1 cut(s)	767
PdiI	GCCGGC	1 cut(s)	2244
PdmI	GAANNNNTTC	1 cut(s)	1512
PfeI	GAWTC	3 cut(s)	413, 1196, 1532
PflMI	CCANNNNNTGG	1 cut(s)	65
PfoI	TCCNGGA	2 cut(s)	241, 532
PleI	GAGTC	2 cut(s)	1631, 2310
PpsI	GAGTC	2 cut(s)	1631, 2310
PpuMI	RGGWCCY	2 cut(s)	146, 2369
PscI	ACATGT	2 cut(s)	503, 1771
Psp124BI	GAGCTC	1 cut(s)	1214
Psp1406I	AACGTT	1 cut(s)	749
Psp5II	RGGWCCY	2 cut(s)	146, 2369
Psp6I	CCWGG	5 cut(s)	241, 532, 856, 1497, 1912
PspFI	CCCAGC	2 cut(s)	330, 1987
PspGI	CCWGG	5 cut(s)	241, 532, 856, 1497, 1912
PspN4I	GGNNCC	6 cut(s)	172, 438, 735, 1219, 1800, 2370
PspPPI	RGGWCCY	2 cut(s)	146, 2369
PstI	CTGCAG	2 cut(s)	76, 400
PstNI	CAGNNNCTG	1 cut(s)	2239
PsuI	RGATCY	2 cut(s)	68, 1235
RruI	TCGCGA	1 cut(s)	90
RsaI	GTAC	5 cut(s)	508, 735, 1702, 1793, 2357
RsaNI	GTAC	5 cut(s)	507, 734, 1701, 1792, 2356
SacI	GAGCTC	1 cut(s)	1214
SaqAI	TTAA	6 cut(s)	98, 570, 726, 1458, 1994, 2108
Sau3AI	GATC	7 cut(s)	68, 536, 1235, 1387, 1564, 2176, 2219
SchI	GAGTC	2 cut(s)	1631, 2310
ScrFI	CCNGG	7 cut(s)	243, 534, 858, 1403, 1499, 1914, 2148
SduI	GDGCHC	4 cut(s)	55, 439, 1099, 1214
SfaNI	GCATC	7 cut(s)	25, 82, 676, 1279, 1416, 1570, 2312
SfcI	CTRYAG	3 cut(s)	72, 396, 903
Sfr274I	CTCGAG	1 cut(s)	1294
SfuI	TTCGAA	1 cut(s)	670
SinI	GGWCC	6 cut(s)	146, 170, 245, 1088, 1897, 2369
SlaI	CTCGAG	1 cut(s)	1294
SmlI	CTYRAG	6 cut(s)	97, 1207, 1294, 1376, 1868, 2318
SmoI	CTYRAG	6 cut(s)	97, 1207, 1294, 1376, 1868, 2318
SphI	GCATGC	2 cut(s)	10, 1186
SseBI	AGGCCT	2 cut(s)	103, 2105
SsiI	CCGC	5 cut(s)	1568, 1942, 2077, 2199, 2353
SspMI	CTAG	7 cut(s)	581, 693, 704, 1731, 2114, 2361, 2424
SstI	GAGCTC	1 cut(s)	1214
StuI	AGGCCT	2 cut(s)	103, 2105
StyD4I	CCNGG	7 cut(s)	241, 532, 856, 1401, 1497, 1912, 2146
StyI	CCWWGG	5 cut(s)	937, 1160, 1600, 1707, 2028
TaaI	ACNGT	6 cut(s)	215, 516, 659, 888, 1334, 1978
TaiI	ACGT	3 cut(s)	752, 1495, 1845
TaqI	TCGA	3 cut(s)	411, 670, 1295
TatI	WGTACW	2 cut(s)	506, 1700
TauI	GCSGC	1 cut(s)	1945
TfiI	GAWTC	3 cut(s)	413, 1196, 1532
Tru1I	TTAA	6 cut(s)	98, 570, 726, 1458, 1994, 2108
Tru9I	TTAA	6 cut(s)	98, 570, 726, 1458, 1994, 2108
TscAI	CASTG	3 cut(s)	649, 1899, 2229
TseFI	GTSAC	5 cut(s)	132, 309, 1661, 1902, 2023
Tsp45I	GTSAC	5 cut(s)	132, 309, 1661, 1902, 2023
TspDTI	ATGAA	8 cut(s)	53, 251, 483, 849, 1238, 1521, 2074, 2177
TspGWI	ACGGA	2 cut(s)	246, 309
TspRI	CASTG	3 cut(s)	649, 1899, 2229
Van91I	CCANNNNNTGG	1 cut(s)	65
Vha464I	CTTAAG	1 cut(s)	97
VpaK11BI	GGWCC	6 cut(s)	146, 170, 245, 1088, 1897, 2369
XapI	RAATTY	6 cut(s)	785, 1111, 1338, 1406, 1450, 2336
XbaI	TCTAGA	1 cut(s)	2423
XceI	RCATGY	5 cut(s)	10, 382, 507, 1186, 1775
XhoI	CTCGAG	1 cut(s)	1294
XmiI	GTMKAC	1 cut(s)	1438
XmnI	GAANNNNTTC	1 cut(s)	1512
XspI	CTAG	7 cut(s)	581, 693, 704, 1731, 2114, 2361, 2424

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.