Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001069

Physical Location & Seq

Genomic Coordinates Reverse (-)

6299211 .. 6304448

Gene Structure 5238 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1079800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3240 bp

ATGCCACCAACTAATTCTCCTCCACCTGCAAGTGTTGAAGGCCATGTACCGCCTATGCCACCAAATAATGCTCCTCCACCACTAAGCATTGAAGTTCATGTACCTTCTGCACCACCAAATAATGCTCCACCGCCAAGTGTCGAAGGCCATGCACTGCCTATGCCACCAAATAATGCTCCTCCACCATTAAGTATTGAAGTTCATGTGCCGTCTGCACCACCAAATACTGCTCCTCCACCACCAAGTGTTGAAGGTCATGTACCGTCTATGCCGCCAACTAATGCTCATCCCCCGCCAATTGTTGAAGGTCATGTACCGTCTATGCCACCAACTGCTTCTCAAACAAAGGCACCAGTTAATAAGAGTCCTATCTCAGTGCCAGTTGCTCCAGTACCCGTTGAAACACCACCCGGGAATTTGCCACAAATTTCACCGGCCATCCATTCAAGTACACCAGAAACTTCACCATTTGCTCCTCAAAGACATGCGCCAGACAATAAGGCTCCCATACCAGAACCAATTGCTCCAGATCCTGAAGTCTCTCCTGCATCATCTGCTCCACCCAGCATCAACGGGAAAAGGAGTCATTTTCCAGTTCTTGCACCACCATATGAAGCTCCCAAGCCATCACTACCCGTGGGTCATACCCCAGCTGAAGCTCCTTCCGTCCGCAAACCTGTGGTTTCATCTCCTCCCCCGAGCATTAACTGGAAAAGGGGTCGAATACCAGTTGTTGCACCATCATACGACACTCCCAAACCATCACCACCCATGGGCCATACCCCAGCTAAAGCTCCTGCTGTCCACAAACCTGTGAGACATTATAAACTTGCTCCTGCACCGTCTATTTCATCCCCTGAACCACCTTTTGATAAAGGGCAACATTCTCCTGCATCTTCACCTTCAATGACTTTCCATAAGAATCATCAAGCGCCATCATATCTGGTTTCTCCTCCCACTCCAGAACAGCCAGGCCCAGTGATGCCACCGGTATCATTTCAAACAGGCAGGCAAAGACACTATGCTCCGCCACCCCTAAATCCAGCGTCTTCAGTCTCTCCATCTCGTTTGCCTGATGCTCCATCAGTGAGCCATTTTTCGCCTGCTCCTTCTCCATCTTTAAAAGGACCAACTCACGAAACCAAATTGCGCCCTAAGATTTCTCCATCTGGAGCTCCAGCAAAGAGCCCAAAAGGGGCACCACCGCTGCCACTAGTTCAAGTATTCCCACCACCACCTCCCAATCAAGATTGTTCGTCAACTATTTGCACCGATCCATATACAAATACCCCTCCTGGGTCACCTTGTGGCTGTGTCTTGCCTCTGCAAGTTGGACTGCGACTTAGTGTCGCACTGTATACCTTCTTTCCCTTGGTTTCAGAGCTAGCCCAGGAAATTGCTGTCGGGGTTCTTATGCAACAAAGTCAGGTCCGCATTATTGGAGCCAATGCAGCTACCCAGCAACCAGACAAGACAGTTGCCCTCATTGACCTGGTTCCCCTCGGGCAAAAGTTCGATAACACCACAGCTTTTTTGACTTCCCAGAGATTTTGGCATAAAGAAGTTGTTATAAACGCATCATACTTTGGCGACTATGAAGTACTATTTGTGCGGTATCCAGGTTTACCCCCGTCTCCACCTTCTTTGGACATTGATGCAATGAATGCGGGGCCGTATCCTGGTAATGACAACAATGGAAGGACGACAAAGCCCCTCGGGGTTGACGTGCACAAGAGGAAGAATAAAAATGGTCTTAGTGGTGGGATAATTGCTATTATTGCTTTGTCTACCTTTGTAGCAGTTGCTTTATGCTCTGCTGCTGCTTGGGTTTTTCTGTTCAAACCTAGAGACCACTCTTCTCAACCAGCAGCAATTCCGCGGGCTACACTACCTTCTTCTGGAAAACCATCAGGTACTGCTGGGTCGATGATGGAAAGTAGGCACAGTTCCTTGTCGTTATCATTCGGATCCAGCAGTGCACCATATACAGGATCCGCTAATACTTTCAGTGCGAGTGATATTGAGAGAGCCACTAACAACTATGATGATTCAAGAGTACTTGGGGAAGGTGGCTTTGGGCGTGTTTATAGTGGCGTTCTTGAAGACGGGACCAAGATTGCCGTCAAAGTTCTAAAAAGAGATGATCATCATGGTGGTCGGGAGTTTTTGGCTGAAGTTGAGATGCTTAGTCGTCTTCATCATCGAAATTTGGTCAAGTTGATTGGCATATGCACAGAAGTGCATAGCCGCTCCTTGGTTTATGAGCTCATTCCTAATGGCAGTGTGGAATCTCATTTACATGGAATTGACAAGGTCAGTGCTCCGCTTAATTGGGTGCAACGCATGAAAATAGCACTTGGGGCTGCTAGGGGGCTGGCGTATCTACACGAGGATTCCAGCCCCCGTGTCATTCACAGGGATTTCAAAACCAGCAATATTTTGCTAGAAGATGATTTTACACCGAAAGTGTCCGACTTTGGTTTGGCGCGAACTGCCATGGATGAGGAAAACAGACACATATCAACACGGGTCATGGGAACTTTTGGGTATGTGGCTCCAGAATATGCAATGACTGGGCATCTTCTTGTCAAGAGTGATGTATACAGCTATGGGGTTGTCCTTCTCGAACTCTTAACTGGAAGAAAACCGGTAGACATGTCACAGCCACCTGGTGAAGAAAACCTAGTTGCATGGGCCCGGCCGCTTCTCACTAGTAAAGAAGGGTTGGAAGCAATCATAGATCCAAATGTAGGATCCGAGGTCCCATTTGAAAGTATCGCCAAAGTGGCAGCTATTGCTTCGATGTGTGTACAGCCAGAGGTATCACACCGCCCTTGTATGGGCGAGGTTGTCCAGGCCTTAAAACTGGTATGCAATGAGTTTGATGAGGCGAAAGAATTTGGTTCAAAGAGTTATAGCCAAGGCGATTTGTCCATTGATGTGGCTGATGACACAAATACTACCTCAGGACAGTTGCCAGATACTTTCCAAAACCAGTATTCCATGCTGACCTATGATTCTGACCTCGAGACAGAGCGGGAAGCATCATTGTCAAATATTTGTAGTACATCAATGAGCACGGGGAGGCAAGATACCGAATCATTCAGGAGGCACTCCAGTTCAGGTCCTTTGGGAACAGGAAGGAGTAAGCAGTTCTGGGAGAGACTTAGGAGATCATCCGGGGGCAGTGTGAGCGAGCACGGGTTTATGTTCAAATTATGGCAAGGCTCCCCCCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010065 GO:0010068 GO:0010154 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0036211 GO:0038023 GO:0042335 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

1080

Amino Acids

115.08

Weight (kDa)

6.89

Isoelectric Point (pI)

63.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALE2_N	PF23180	424 - 543	4.1e-58	Receptor-like PK ALE2 N-terminal domain
Pkinase	PF00069	681 - 951	6.8e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	685 - 954	4.8e-48	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001069)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G56890
fragaria_vesca	FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_1g07530 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370 FvH4_6g33370
malus_domestica	MD02G1079800.v1.1 MD09G1193100.v1.1 MD15G1207500.v1.1
prunus_persica	Prupe.3G049500_v2.0.a1 Prupe.7G209300_v2.0.a1 Prupe.7G209300_v2.0.a1
pyrus_communis	pycom02g06360 pycom09g10970 pycom15g18390
rosa_chinensis	RchiOBHm_Chr2g0093411 RchiOBHm_Chr2g0143231
rosa_laevigata	RLG00000016396 RLG00000020017 RLG00000020021
rosa_multiflora	Rmu_co8519777.1_g000001 Rmu_sc0003950.1_g000003
rosa_roxburghii	Rroxscaffold_2G00102000 Rroxscaffold_2G00148130
rosa_rugosa	Rorug02G0035200 Rorug02G0035200 Rorug02G0035300 Rorug02G0035400 Rorug02G0035500 Rorug02G0035600 Rorug02G0379700
rosa_samantha	Rh2AG081600 Rh2AG432500 Rh2BG082900 Rh2BG441100 Rh2CG084900 Rh2CG418300 Rh2DG080600 Rh2DG451200
rosa_wichuraiana	Rw2G006500 Rw2G035310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	825, 1571
AarI	CACCTGC	1 cut(s)	34
Acc36I	ACCTGC	1 cut(s)	34
AccB1I	GGYRCC	2 cut(s)	349, 1198
AccBSI	CCGCTC	2 cut(s)	2250, 3037
AccI	GTMKAC	4 cut(s)	1358, 1787, 2601, 2652
AccII	CGCG	2 cut(s)	1879, 2488
AclWI	GGATC	9 cut(s)	524, 1268, 1962, 1975, 1986, 1999, 2735, 2748, 2761
AcoI	YGGCCR	2 cut(s)	435, 2699
AcsI	RAATTY	4 cut(s)	415, 426, 2206, 2897
AcuI	CTGAAG	4 cut(s)	555, 675, 1035, 2193
AdeI	CACNNNGTG	3 cut(s)	245, 1307, 2672
AflIII	ACRYGT	1 cut(s)	2655
AgeI	ACCGGT	2 cut(s)	988, 2647
AhdI	GACNNNNNGTC	1 cut(s)	1346
AhlI	ACTAGT	2 cut(s)	1213, 2711
AjiI	CACGTC	1 cut(s)	1726
AjnI	CCWGG	8 cut(s)	970, 1294, 1389, 1491, 1618, 1678, 2668, 2853
AjuI	GAANNNNNNNTTGG	2 cut(s)	2058, 2090
AleI	CACNNNNGTG	1 cut(s)	2237
Alw21I	GWGCWC	7 cut(s)	1177, 1731, 1981, 2268, 2323, 3080, 3201
Alw26I	GTCTC	7 cut(s)	544, 811, 1060, 1638, 1842, 3023, 3157
Alw44I	GTGCAC	2 cut(s)	1727, 1977
AlwI	GGATC	9 cut(s)	524, 1268, 1962, 1975, 1986, 1999, 2735, 2748, 2761
AlwNI	CAGNNNCTG	2 cut(s)	533, 1916
Ama87I	CYCGRG	5 cut(s)	410, 697, 1502, 1715, 3026
AoxI	GGCC	9 cut(s)	40, 145, 435, 775, 973, 1670, 2694, 2699, 2856
ApaI	GGGCCC	1 cut(s)	2698
ApaLI	GTGCAC	2 cut(s)	1727, 1977
ApeKI	GCWGC	7 cut(s)	1207, 1451, 1817, 1820, 1868, 2363, 2789
ApoI	RAATTY	4 cut(s)	415, 426, 2206, 2897
AsiGI	ACCGGT	2 cut(s)	988, 2647
AspLEI	GCGC	4 cut(s)	490, 934, 1152, 2488
AsuC2I	CCSGG	4 cut(s)	411, 412, 2698, 3181
AsuHPI	GGTGA	6 cut(s)	423, 456, 756, 891, 1293, 2684
AsuNHI	GCTAGC	1 cut(s)	1384
AvaI	CYCGRG	5 cut(s)	410, 697, 1502, 1715, 3026
AvaII	GGWCC	5 cut(s)	1127, 1429, 2109, 2761, 3125
AxyI	CCTNAGG	1 cut(s)	2965
BaeGI	GKGCMC	4 cut(s)	1201, 1731, 1981, 2698
BamHI	GGATCC	3 cut(s)	1967, 1991, 2753
BanI	GGYRCC	2 cut(s)	349, 1198
BanII	GRGCYC	4 cut(s)	1177, 1190, 2268, 2698
BarI	GAAGNNNNNNTAC	8 cut(s)	30, 62, 84, 116, 243, 275, 297, 329
BauI	CACGAG	1 cut(s)	2387
BbsI	GAAGAC	3 cut(s)	1041, 2109, 2186
Bbv12I	GWGCWC	7 cut(s)	1177, 1731, 1981, 2268, 2323, 3080, 3201
BbvI	GCAGC	7 cut(s)	1194, 1463, 1804, 1807, 1880, 2350, 2801
BceAI	ACGGC	3 cut(s)	193, 1657, 2105
BcgI	CGANNNNNNTGC	2 cut(s)	131, 165
BciT130I	CCWGG	8 cut(s)	972, 1296, 1391, 1493, 1620, 1680, 2670, 2855
BciVI	GTATCC	2 cut(s)	1626, 1686
BclI	TGATCA	1 cut(s)	2143
BcnI	CCSGG	4 cut(s)	411, 412, 2698, 3181
BcoDI	GTCTC	7 cut(s)	544, 811, 1060, 1638, 1842, 3023, 3157
BcuI	ACTAGT	2 cut(s)	1213, 2711
BfaI	CTAG	7 cut(s)	1214, 1385, 1845, 2367, 2444, 2684, 2712
BfoI	RGCGCY	1 cut(s)	935
BfuAI	ACCTGC	1 cut(s)	34
BfuI	GTATCC	2 cut(s)	1626, 1686
BglI	GCCNNNNNGGC	1 cut(s)	2786
BmcAI	AGTACT	2 cut(s)	1602, 2058
Bme18I	GGWCC	5 cut(s)	1127, 1429, 2109, 2761, 3125
BmeRI	GACNNNNNGTC	1 cut(s)	1346
BmeT110I	CYCGRG	5 cut(s)	410, 697, 1502, 1715, 3026
BmgBI	CACGTC	1 cut(s)	1726
BmrI	ACTGGG	2 cut(s)	971, 2583
BmtI	GCTAGC	1 cut(s)	1388
BmuI	ACTGGG	2 cut(s)	971, 2583
BpiI	GAAGAC	3 cut(s)	1041, 2109, 2186
BpmI	CTGGAG	7 cut(s)	372, 510, 945, 1161, 1191, 2541, 3100
BpuMI	CCSGG	4 cut(s)	411, 412, 2698, 3181
BsaBI	GATNNNNATC	1 cut(s)	2145
BsaI	GGTCTC	1 cut(s)	1842
BsaWI	WCCGGW	2 cut(s)	988, 2647
BsaXI	ACNNNNNCTCC	3 cut(s)	31, 217, 247
Bse118I	RCCGGY	3 cut(s)	433, 988, 2647
Bse21I	CCTNAGG	1 cut(s)	2965
Bse3DI	GCAATG	3 cut(s)	1665, 2574, 2881
Bse8I	GATNNNNATC	1 cut(s)	2145
BseBI	CCWGG	8 cut(s)	972, 1296, 1391, 1493, 1620, 1680, 2670, 2855
BseGI	GGATG	5 cut(s)	286, 438, 851, 2506, 3176
BseJI	GATNNNNATC	1 cut(s)	2145
BseMI	GCAATG	3 cut(s)	1665, 2574, 2881
BseMII	CTCAG	2 cut(s)	387, 2979
BseRI	GAGGAG	7 cut(s)	9, 63, 168, 222, 465, 681, 942
BseSI	GKGCMC	4 cut(s)	1201, 1731, 1981, 2698
BseX3I	CGGCCG	1 cut(s)	2699
BseXI	GCAGC	7 cut(s)	1194, 1463, 1804, 1807, 1880, 2350, 2801
BseYI	CCCAGC	5 cut(s)	563, 649, 784, 1458, 1919
BsgI	GTGCAG	3 cut(s)	93, 198, 822
Bsh1236I	CGCG	2 cut(s)	1879, 2488
Bsh1285I	CGRYCG	1 cut(s)	2702
BshFI	GGCC	9 cut(s)	42, 147, 437, 777, 975, 1672, 2696, 2701, 2858
BshNI	GGYRCC	2 cut(s)	349, 1198
BshTI	ACCGGT	2 cut(s)	988, 2647
BsiEI	CGRYCG	1 cut(s)	2702
BsiHKAI	GWGCWC	7 cut(s)	1177, 1731, 1981, 2268, 2323, 3080, 3201
BsiHKCI	CYCGRG	5 cut(s)	410, 697, 1502, 1715, 3026
BsiSI	CCGG	6 cut(s)	411, 434, 989, 2648, 2698, 3180
BslFI	GGGAC	2 cut(s)	2122, 2747
BsmAI	GTCTC	7 cut(s)	544, 811, 1060, 1638, 1842, 3023, 3157
BsmBI	CGTCTC	1 cut(s)	1638
BsmFI	GGGAC	2 cut(s)	2122, 2747
BsmI	GAATGC	1 cut(s)	1669
BsnI	GGCC	9 cut(s)	42, 147, 437, 777, 975, 1672, 2696, 2701, 2858
Bso31I	GGTCTC	1 cut(s)	1842
BsoBI	CYCGRG	5 cut(s)	410, 697, 1502, 1715, 3026
Bsp120I	GGGCCC	1 cut(s)	2694
Bsp1407I	TGTACA	1 cut(s)	2809
Bsp143I	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
Bsp19I	CCATGG	2 cut(s)	771, 2496
BspANI	GGCC	9 cut(s)	42, 147, 437, 777, 975, 1672, 2696, 2701, 2858
BspCNI	CTCAG	2 cut(s)	386, 2978
BspFNI	CGCG	2 cut(s)	1879, 2488
BspMI	ACCTGC	1 cut(s)	34
BspOI	GCTAGC	1 cut(s)	1388
BspPI	GGATC	9 cut(s)	524, 1268, 1962, 1975, 1986, 1999, 2735, 2748, 2761
BspT107I	GGYRCC	2 cut(s)	349, 1198
BspTNI	GGTCTC	1 cut(s)	1842
BsrBI	CCGCTC	2 cut(s)	2250, 3037
BsrDI	GCAATG	3 cut(s)	1665, 2574, 2881
BsrFI	RCCGGY	3 cut(s)	433, 988, 2647
BsrGI	TGTACA	1 cut(s)	2809
BssAI	RCCGGY	3 cut(s)	433, 988, 2647
BssMI	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
BssNAI	GTATAC	2 cut(s)	1359, 2602
BssSI	CACGAG	1 cut(s)	2387
BssT1I	CCWWGG	5 cut(s)	771, 1371, 2253, 2496, 2920
Bst1107I	GTATAC	2 cut(s)	1359, 2602
Bst2BI	CACGAG	1 cut(s)	2387
Bst2UI	CCWGG	8 cut(s)	972, 1296, 1391, 1493, 1620, 1680, 2670, 2855
Bst4CI	ACNGT	7 cut(s)	264, 318, 843, 1356, 1477, 1946, 2973
Bst6I	CTCTTC	1 cut(s)	1861
BstAPI	GCANNNNNTGC	3 cut(s)	554, 1664, 2795
BstAUI	TGTACA	1 cut(s)	2809
BstC8I	GCNNGC	6 cut(s)	1010, 1104, 1386, 1881, 2376, 3197
BstDEI	CTNAG	8 cut(s)	83, 373, 1155, 1343, 1754, 2186, 2965, 3167
BstDSI	CCRYGG	4 cut(s)	636, 771, 1877, 2496
BstEII	GGTNACC	1 cut(s)	1299
BstF5I	GGATG	5 cut(s)	286, 438, 851, 2506, 3176
BstFNI	CGCG	2 cut(s)	1879, 2488
BstH2I	RGCGCY	1 cut(s)	935
BstHHI	GCGC	4 cut(s)	490, 934, 1152, 2488
BstKTI	GATC	8 cut(s)	532, 1276, 1970, 1994, 2146, 2743, 2756, 3176
BstMAI	GTCTC	7 cut(s)	544, 811, 1060, 1638, 1842, 3023, 3157
BstMBI	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
BstMCI	CGRYCG	1 cut(s)	2702
BstMWI	GCNNNNNNNGC	9 cut(s)	554, 1451, 1664, 1778, 2360, 2492, 2786, 2795, 3192
BstNI	CCWGG	8 cut(s)	972, 1296, 1391, 1493, 1620, 1680, 2670, 2855
BstNSI	RCATGY	2 cut(s)	488, 2659
BstPI	GGTNACC	1 cut(s)	1299
BstSLI	GKGCMC	4 cut(s)	1201, 1731, 1981, 2698
BstUI	CGCG	2 cut(s)	1879, 2488
BstV1I	GCAGC	7 cut(s)	1194, 1463, 1804, 1807, 1880, 2350, 2801
BstV2I	GAAGAC	3 cut(s)	1041, 2109, 2186
BstX2I	RGATCY	5 cut(s)	529, 1967, 1991, 2740, 2753
BstXI	CCANNNNNNTGG	1 cut(s)	2941
BstYI	RGATCY	5 cut(s)	529, 1967, 1991, 2740, 2753
BstZ17I	GTATAC	2 cut(s)	1359, 2602
BstZI	CGGCCG	1 cut(s)	2699
Bsu36I	CCTNAGG	1 cut(s)	2965
BsuI	GTATCC	2 cut(s)	1626, 1686
BsuRI	GGCC	9 cut(s)	42, 147, 437, 777, 975, 1672, 2696, 2701, 2858
BtgI	CCRYGG	4 cut(s)	636, 771, 1877, 2496
BtrI	CACGTC	1 cut(s)	1726
BtsCI	GGATG	5 cut(s)	286, 438, 851, 2506, 3176
BtsI	GCAGTG	4 cut(s)	152, 1981, 2287, 3193
BveI	ACCTGC	1 cut(s)	34
Cac8I	GCNNGC	6 cut(s)	1010, 1104, 1386, 1881, 2376, 3197
CaiI	CAGNNNCTG	2 cut(s)	533, 1916
CfoI	GCGC	4 cut(s)	490, 934, 1152, 2488
Cfr10I	RCCGGY	3 cut(s)	433, 988, 2647
Cfr42I	CCGCGG	1 cut(s)	1880
Cfr9I	CCCGGG	1 cut(s)	410
CseI	GACGC	1 cut(s)	1035
CsiI	ACCWGGT	2 cut(s)	1491, 2668
CspAI	ACCGGT	2 cut(s)	988, 2647
CspCI	CAANNNNNGTGG	2 cut(s)	1626, 1661
DdeI	CTNAG	8 cut(s)	83, 373, 1155, 1343, 1754, 2186, 2965, 3167
DpnI	GATC	8 cut(s)	531, 1275, 1969, 1993, 2145, 2742, 2755, 3175
DpnII	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
DraI	TTTAAA	1 cut(s)	1122
DraIII	CACNNNGTG	3 cut(s)	245, 1307, 2672
DriI	GACNNNNNGTC	1 cut(s)	1346
EaeI	YGGCCR	2 cut(s)	435, 2699
EagI	CGGCCG	1 cut(s)	2699
Eam1104I	CTCTTC	1 cut(s)	1861
Eam1105I	GACNNNNNGTC	1 cut(s)	1346
EarI	CTCTTC	1 cut(s)	1861
EciI	GGCGGA	1 cut(s)	1017
Ecl136II	GAGCTC	2 cut(s)	1175, 2266
EclXI	CGGCCG	1 cut(s)	2699
Eco130I	CCWWGG	5 cut(s)	771, 1371, 2253, 2496, 2920
Eco147I	AGGCCT	1 cut(s)	2858
Eco24I	GRGCYC	4 cut(s)	1177, 1190, 2268, 2698
Eco31I	GGTCTC	1 cut(s)	1842
Eco47I	GGWCC	5 cut(s)	1127, 1429, 2109, 2761, 3125
Eco52I	CGGCCG	1 cut(s)	2699
Eco53kI	GAGCTC	2 cut(s)	1175, 2266
Eco57I	CTGAAG	4 cut(s)	555, 675, 1035, 2193
Eco81I	CCTNAGG	1 cut(s)	2965
Eco88I	CYCGRG	5 cut(s)	410, 697, 1502, 1715, 3026
Eco91I	GGTNACC	1 cut(s)	1299
EcoICRI	GAGCTC	2 cut(s)	1175, 2266
EcoO109I	RGGNCCY	2 cut(s)	2761, 3125
EcoO65I	GGTNACC	1 cut(s)	1299
EcoRII	CCWGG	8 cut(s)	970, 1294, 1389, 1491, 1618, 1678, 2668, 2853
EcoT14I	CCWWGG	5 cut(s)	771, 1371, 2253, 2496, 2920
EcoT38I	GRGCYC	4 cut(s)	1177, 1190, 2268, 2698
ErhI	CCWWGG	5 cut(s)	771, 1371, 2253, 2496, 2920
Esp3I	CGTCTC	1 cut(s)	1638
FaqI	GGGAC	2 cut(s)	2122, 2747
FauI	CCCGC	4 cut(s)	300, 1660, 1872, 3030
FauNDI	CATATG	2 cut(s)	610, 2228
FbaI	TGATCA	1 cut(s)	2143
FblI	GTMKAC	4 cut(s)	1358, 1787, 2601, 2652
FokI	GGATG	5 cut(s)	273, 425, 838, 2513, 3163
FriOI	GRGCYC	4 cut(s)	1177, 1190, 2268, 2698
FspBI	CTAG	7 cut(s)	1214, 1385, 1845, 2367, 2444, 2684, 2712
GlaI	GCGC	4 cut(s)	489, 933, 1151, 2487
GsaI	CCCAGC	5 cut(s)	567, 653, 788, 1462, 1923
GsuI	CTGGAG	7 cut(s)	372, 510, 945, 1161, 1191, 2541, 3100
HaeII	RGCGCY	1 cut(s)	935
HaeIII	GGCC	9 cut(s)	42, 147, 437, 777, 975, 1672, 2696, 2701, 2858
HapII	CCGG	6 cut(s)	411, 434, 989, 2648, 2698, 3180
HgaI	GACGC	1 cut(s)	1035
HhaI	GCGC	4 cut(s)	490, 934, 1152, 2488
Hin6I	GCGC	4 cut(s)	488, 932, 1150, 2486
HinP1I	GCGC	4 cut(s)	488, 932, 1150, 2486
HincII	GTYRAC	2 cut(s)	1260, 1723
HindII	GTYRAC	2 cut(s)	1260, 1723
HinfI	GANTC	8 cut(s)	364, 583, 922, 2048, 2288, 2393, 3017, 3098
HpaII	CCGG	6 cut(s)	411, 434, 989, 2648, 2698, 3180
HphI	GGTGA	6 cut(s)	423, 456, 756, 891, 1293, 2684
Hpy188I	TCNGA	5 cut(s)	1381, 1967, 2473, 2758, 3022
HpyCH4III	ACNGT	7 cut(s)	264, 318, 843, 1356, 1477, 1946, 2973
HpyCH4IV	ACGT	1 cut(s)	1725
HpyF10VI	GCNNNNNNNGC	9 cut(s)	554, 1451, 1664, 1778, 2360, 2492, 2786, 2795, 3192
HpyF3I	CTNAG	8 cut(s)	83, 373, 1155, 1343, 1754, 2186, 2965, 3167
HpySE526I	ACGT	1 cut(s)	1725
HspAI	GCGC	4 cut(s)	488, 932, 1150, 2486
Ksp22I	TGATCA	1 cut(s)	2143
KspI	CCGCGG	1 cut(s)	1880
Kzo9I	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
Lsp1109I	GCAGC	7 cut(s)	1194, 1463, 1804, 1807, 1880, 2350, 2801
MabI	ACCWGGT	2 cut(s)	1491, 2668
MaeI	CTAG	7 cut(s)	1214, 1385, 1845, 2367, 2444, 2684, 2712
MaeII	ACGT	1 cut(s)	1725
MaeIII	GTNAC	2 cut(s)	1299, 2658
MalI	GATC	8 cut(s)	531, 1275, 1969, 1993, 2145, 2742, 2755, 3175
MbiI	CCGCTC	2 cut(s)	2250, 3037
MboI	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
MfeI	CAATTG	2 cut(s)	297, 519
MflI	RGATCY	5 cut(s)	529, 1967, 1991, 2740, 2753
MlyI	GAGTC	2 cut(s)	373, 592
MmeI	TCCRAC	3 cut(s)	1312, 2496, 2706
MseI	TTAA	7 cut(s)	188, 357, 705, 1121, 2328, 2633, 2861
MslI	CAYNNNNRTG	4 cut(s)	2151, 2237, 2298, 2939
MspA1I	CMGCKG	3 cut(s)	653, 1207, 1879
MspI	CCGG	6 cut(s)	411, 434, 989, 2648, 2698, 3180
MunI	CAATTG	2 cut(s)	297, 519
Mva1269I	GAATGC	1 cut(s)	1669
MvaI	CCWGG	8 cut(s)	972, 1296, 1391, 1493, 1620, 1680, 2670, 2855
MvnI	CGCG	2 cut(s)	1879, 2488
MwoI	GCNNNNNNNGC	9 cut(s)	554, 1451, 1664, 1778, 2360, 2492, 2786, 2795, 3192
NciI	CCSGG	4 cut(s)	411, 412, 2698, 3181
NcoI	CCATGG	2 cut(s)	771, 2496
NdeI	CATATG	2 cut(s)	610, 2228
NdeII	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
NheI	GCTAGC	1 cut(s)	1384
NmuCI	GTSAC	2 cut(s)	1299, 2658
NspI	RCATGY	2 cut(s)	488, 2659
OliI	CACNNNNGTG	1 cut(s)	2237
PaeR7I	CTCGAG	1 cut(s)	3026
PaqCI	CACCTGC	1 cut(s)	34
PceI	AGGCCT	1 cut(s)	2858
PciI	ACATGT	1 cut(s)	2655
PcsI	WCGNNNNNNNCGW	1 cut(s)	1722
PctI	GAATGC	1 cut(s)	1669
PfeI	GAWTC	6 cut(s)	922, 2048, 2288, 2393, 3017, 3098
PflFI	GACNNNGTC	1 cut(s)	2312
PinAI	ACCGGT	2 cut(s)	988, 2647
PleI	GAGTC	2 cut(s)	372, 591
PpsI	GAGTC	2 cut(s)	372, 591
PpuMI	RGGWCCY	2 cut(s)	2761, 3125
PscI	ACATGT	1 cut(s)	2655
PsiI	TTATAA	2 cut(s)	825, 1571
Psp124BI	GAGCTC	2 cut(s)	1177, 2268
Psp5II	RGGWCCY	2 cut(s)	2761, 3125
Psp6I	CCWGG	8 cut(s)	970, 1294, 1389, 1491, 1618, 1678, 2668, 2853
PspEI	GGTNACC	1 cut(s)	1299
PspFI	CCCAGC	5 cut(s)	563, 649, 784, 1458, 1919
PspGI	CCWGG	8 cut(s)	970, 1294, 1389, 1491, 1618, 1678, 2668, 2853
PspOMI	GGGCCC	1 cut(s)	2694
PspPPI	RGGWCCY	2 cut(s)	2761, 3125
PsrI	GAACNNNNNNTAC	2 cut(s)	1930, 1962
PstNI	CAGNNNCTG	2 cut(s)	533, 1916
PsuI	RGATCY	5 cut(s)	529, 1967, 1991, 2740, 2753
PsyI	GACNNNGTC	1 cut(s)	2312
PvuII	CAGCTG	1 cut(s)	653
RseI	CAYNNNNRTG	4 cut(s)	2151, 2237, 2298, 2939
SacI	GAGCTC	2 cut(s)	1177, 2268
SacII	CCGCGG	1 cut(s)	1880
SaqAI	TTAA	7 cut(s)	188, 357, 705, 1121, 2328, 2633, 2861
Sau3AI	GATC	8 cut(s)	529, 1273, 1967, 1991, 2143, 2740, 2753, 3173
ScaI	AGTACT	2 cut(s)	1602, 2058
SchI	GAGTC	2 cut(s)	373, 592
SexAI	ACCWGGT	2 cut(s)	1491, 2668
Sfr274I	CTCGAG	1 cut(s)	3026
Sfr303I	CCGCGG	1 cut(s)	1880
SgrBI	CCGCGG	1 cut(s)	1880
SinI	GGWCC	5 cut(s)	1127, 1429, 2109, 2761, 3125
SlaI	CTCGAG	1 cut(s)	3026
SmaI	CCCGGG	1 cut(s)	412
SmiMI	CAYNNNNRTG	4 cut(s)	2151, 2237, 2298, 2939
SmlI	CTYRAG	1 cut(s)	3026
SmoI	CTYRAG	1 cut(s)	3026
SpeI	ACTAGT	2 cut(s)	1213, 2711
SseBI	AGGCCT	1 cut(s)	2858
SspI	AATATT	2 cut(s)	2437, 3058
SspMI	CTAG	7 cut(s)	1214, 1385, 1845, 2367, 2444, 2684, 2712
SstI	GAGCTC	2 cut(s)	1177, 2268
StuI	AGGCCT	1 cut(s)	2858
StyI	CCWWGG	5 cut(s)	771, 1371, 2253, 2496, 2920
TaaI	ACNGT	7 cut(s)	264, 318, 843, 1356, 1477, 1946, 2973
TaiI	ACGT	1 cut(s)	1728
TaqI	TCGA	8 cut(s)	141, 721, 1515, 1925, 2203, 2625, 2801, 3027
TatI	WGTACW	5 cut(s)	449, 1600, 2056, 2809, 3065
TauI	GCSGC	3 cut(s)	274, 2250, 2704
TfiI	GAWTC	6 cut(s)	922, 2048, 2288, 2393, 3017, 3098
Tru1I	TTAA	7 cut(s)	188, 357, 705, 1121, 2328, 2633, 2861
Tru9I	TTAA	7 cut(s)	188, 357, 705, 1121, 2328, 2633, 2861
TseFI	GTSAC	2 cut(s)	1299, 2658
TseI	GCWGC	7 cut(s)	1207, 1451, 1817, 1820, 1868, 2363, 2789
Tsp45I	GTSAC	2 cut(s)	1299, 2658
TspDTI	ATGAA	9 cut(s)	86, 191, 627, 675, 840, 1611, 1676, 2186, 2360
TspGWI	ACGGA	1 cut(s)	655
TspMI	CCCGGG	1 cut(s)	410
Tth111I	GACNNNGTC	1 cut(s)	2312
VneI	GTGCAC	2 cut(s)	1727, 1977
VpaK11BI	GGWCC	5 cut(s)	1127, 1429, 2109, 2761, 3125
XapI	RAATTY	4 cut(s)	415, 426, 2206, 2897
XceI	RCATGY	2 cut(s)	488, 2659
XhoI	CTCGAG	1 cut(s)	3026
XmaI	CCCGGG	1 cut(s)	410
XmiI	GTMKAC	4 cut(s)	1358, 1787, 2601, 2652
XspI	CTAG	7 cut(s)	1214, 1385, 1845, 2367, 2444, 2684, 2712
ZrmI	AGTACT	2 cut(s)	1602, 2058

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.