FvH4_6g34630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004044
Physical Location & Seq
Forward (+)
27306076 .. 27317901
11826 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g34630.t6

Sequence Viewer

Length: 5634 bp
ATGGACTCCCTCCTTAACTCCGCCATCGAAGAAATCTGCTCGCACCTCCACGACGGCCTCTCTCTCCAAACCCTCTGGTCCCGCCTCGACCCTTCCCTCTCCTCCTCTCCCTCCCTCAAACAATCCCTATGGGACTCACTCCGCTCCGTCCCCACCCTCAAATTCCTCGCCCCCCAAAATTACCATTACGCCCCCGCCGACCCTTCCATCCACTCCTTCCAAGCCGCCGAGAATCTCAATCTGAAACTCGTCGCCGACGAGTCCCTCCGCAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAACTCCAACTTGTCCTCCGTCCAACGCCACACTCTCCAACGCGTCGCCATGGCCAGGACCAATGGGATTACGCAGACTCAGCTTGCGAAGGAGCTGGGCATTGAGGGGAGGAACTTCCATTATGCTGTGAAGAATCTCGAGTGTCAAGGCTTACTTGTGCGGAAATCTGCGCTGTTGAGGTTAAAAGAAGCTGGTGATGAGGGGGAGCCGAGGAACAATCCGAGTGTGACCACTAATATGTTGTACTTGTATCGGCACGCAAAGCATTTGAGTGCCCAGCAGAAGATTGAGATCATAAAGGAAGAGAGAGCTGAGGAGAGTTTTGTAAATGTGACAGAAAGCGAAAAAGGAGATGTTTCTGCTGGAAGTGTGCTTGTCAAGGACTTTCTACCGGCAATGAAAGCAGTGTGTGATAAACTTGAAGAAGCAAATGGCAAGGTTCTTGTCGTTGGGGATATTAAGAAGGAACTTGGTTACTGTGGAACTTCAGGGCATAGAGCTTGGAGAAATATTTGTCAGAGGTTGAAAGCTGCTCAGCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGAAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAACGAGAGTTGATCCAAGAACAGTTGTACATGTAGATGAGGATTTTGTTGAAGAACAAAACGTGAAATTCGGAAAGAAATGTAAAATCACTGACCAACTTGTAGAACTTCCTATTGAGCATCAAATTTATGAAATAATAGATGCTGCAGGATCAGAGGGCTTGACCAGGAATGAGGTTATGGAGAGACTTGGAATTGACAACAAGAAAAATTATGCCCGCTTTGTTACTATGTGCTCAAGATTTGAGATGAGTCTGCAACCAGAAATGCACAAAAAAGCCGTGGCATATCGATTTCGGACATCGGGGAAACATGAATCTGAATCAATTAATGCATTTCTTCAAAAATCGAAAGATGCTAATGACGGTAAACTTTCTAGCCTATATGATGGAAGTGTAGATGCTCTGAAGTCTGATCAATTTCAACCCGGCTCAGTGTCAGATTGTTTGTCTCTGAAAGGAGTAACTGCTGGCCCTGAAAATATCAATAACACAGAGGCTAATACTGATCCTTCTGCTGGATCTCTTGGGTGTAATGAGTTGTACAATATGCCGGAAACTTCCCAACAATTATTTCTTGGACCAAAAGATACTACTTCTGATTCTCAAGTTAGTTTAGCAAGCACAGGAGTGGAAACAAATAGCGCCTTATCAGAAAGACCAGCTGCTTTGTCAAAGCCACTGAGTAAAGGCTCAGATCCGAGGTATCCATGTTTGTCTTTGACCGTGGATAACACTAGAAGGGAAAAGAGGATAGTTGAACGGCTAGAGGGCGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGATAAGTGCACAGCAACTGACAGGAAAACAATCGACCGAATTTTACATAAACTTCAACAGCTAGGGCACTGCAAATGTATAGATATTAGCGTCCCTGTTGTCACAAATTTGGGCCGTTCCCGAACCACTGTAGTGGTTCTGCACCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCATGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAGTGGACCAGTTCCTGTACTAGAAGATGTTCAGAGAACCCAGACCCATCTTAGCGCACACCGACAAACAGTGAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTGCTGCATAGTTTCCTGTGGGAATACCTGTATGGTTCGTCTGGCTCTAATGATGCTTTATTATCTGGAAAAGATGTTATTGAGCCGAAAGATCCTTGCAGTACTAGCAAATTGTTTTCGCTAGAGGCAACAATGAAGGCCATTCCAGTTGAATTGTTTCTACAAGTTGCAGGATCCACCAAAAACTTTGAAGATATGATTGAGAAGTGTAAGAGGGGTTTATGTCTCTCTGATCTCTCTAGTAAAGAATACAAGTCTCTGATGGATAGCCATGCAACTGGAAGGCTCTCATTGGTGATTGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGACCACCGCTCAGAAAATGGACTCCAAGTTCATCCTCCCATTATATCTGCATATGCATTGGAACTTAAGCCTTATATAGAGGAACCTGTTTCAAAAGATGCTATATCTTTGAGATTTGGATCTCTTGATCTTCGTCCACGAATCAGACATGATTTTTCCCTATCAAACAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCGGACCCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAACGTGATGAGCTGTTAAAGCGTGTTGTGAAGGATGATCCAAGCGAAAAGCTTTCATTCAAAGAGTGTGGGAAGATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACATGGATGAAGTTCAACCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATGATGAGGTAAACGGACAGCTGGAAGAACAGACACATCCCACAGTATCTGATACCGTGGAACAATTAGAAGAACTAAATTTCTTGGTCACTTATGAGCATGATTCACGTTTGCAAGCATTGAACAATTGTCTAGAAACTGGGCAGGAAGCTCAACCAAACGAAGATAATGAGGGCTGCCATTCAATTACTACCAAGGGGTCTTCTTCAAAATTGAAGCCAAGATGTTCTCGTAAAAGAAACAAGTTGCATACAACACGTCAAAGAAGGTTTTCTTGGACAGAGGAGGCTGATAGGAACTTGATTATCCAATATGTAAGACATCGTGCAACTCTTGGGGCAAAGATTCACCGGGTAAATTGGGCTTCAGTTCCTGATCTCCCAGCACCTCCTGTTGCCTGCATGAAAAGAATGGCATCACTAAAAAGTAACAAAAAGTTTAGATCAGCTGTAATGAGGCTTTGTAACATTCTCAGTGAACGATATGCAAGGATCTTAAAAAAGACTCAGAATAGGTCACTGAACAAAGATGATTGCAGTCTGCTTTTGCGAGATTCTATAGGTGAAGGTCATGACAACAATCTCCCCAATATCAGTGATCAAAATCTAGGGACAGCTCTTCAGAATGAACCATGGGATGATTTTGATGACAATTATGTTAAAAAGTCCCTTGAGGAGGTACTTCACCACAAGAGGTTGGCCAAATTTGATGCCTCTACCAGAGTTGGATCTACCAGTGAGGACCGGACAGATCTTAACACAAGTGAATATGATCCCCCGGAATCTGAATTGATTGCATCAACTGCTCCATATGAGGATGTTCAGAATCATGGTGGCAGAGAAAAAAAATCAGCACGAAGATCTAACTATCAACACCTTAATGAAAAGTACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACACAAGTCTACAAATCATTAGCTGTTTCGAATGCTGTAGAGCTGTTTAAGCTTGTTTTCTTGAGTACCTCAACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCGTTCTACGGCGGTACTCGGAGTGTGATCTATTTGCAGCTTTCAACTATCTTAGAGATAAAAAATTCATGGTTGGGGGTAATGGCAGTCAAAAATTTTCCCTCTCACAACAGTTTTTGCATAGTACCTCCGCATCACCATTTCCAACTAATTCTGGGAAGAGAGCTACTAAATTTGCCCATTTTATCCACGAAAAAGATAAACATCTTATGGAAGGGGGGATTGATCTCTCCACAGATTTACAGTGTGGGGAGATATTCCATTTGTTTGCTCTAGTTTCTTCAGGTGAACTGTCAATTTCCCCATGCTTGCCAGATGAAGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCCGATATCAATGAACTTTTAGATGATGAAAGGACTAAAAAGCTAAAATCATTTGTGGCTGCAGAAGGTGAAATTATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCTCAGTCTCTGTAAGTCGTAAAGAATTCTCTACAGCTAATTGTATAGATTTGTTCAAGGAAGACACTCCTATTGGTGAAAAGCATTTTGGGGGAAGTCAACACCTAGAATGTACTTCTGTTGGAAGTAGCTTATCCCATTCTGACTGTATGAAAGAAATCTTCAGTTCAGGCAGTACTGCCCCTGTATTAGAGCTTGGTTGCGACTCACCTTGGGAAGGTATGGTGGGCTATGCTGGACATTTGTTCCCATTACATTCTGCTCAAGATCAATCCAGTCCCATCCGTCCGGAGGTTTTTAAAGCTGTTTACACTGCTATTCAGAAAGCTGGGGATCAAGGTCTGAGCATAGAAGAAGTTTCCCGAATAACAAATATCCCAGGAGAAAAGATGACTGATGTTATCATTGATGTACTTCAGACATTTGAACGAGTCTTAAAGGTCAATGCTTATGACTCTATTCGTGTTGTGGATTCTTTGTATCGGGGCAAGTATTTTATGACTTCAGTGTCCGGAGTTGATCGAAAACTTGAACCACCTTCATGGAGAAAGCCACAAGGGAAAAATGATGACCATATAGTTATCCATTCAGAAAATTGTGATACTGGTGCTGCTCCAGAGAGGGAGATAAATGCGGATGTCCATAAATTGACAATTCTTAATTTCCCTGAAGAGGTTGATGAGCTCTTGTATGAAAAACAAACTGAAAGCTATAGGGAAAGTAAAGGAGGAGATGCTGAGGATGAATCTTCTAGATCCTCTAATGATAGACTATGCATGCCTATATTCCCATGGATAAATGGTGATGGTACCACCAACAAGATTGTCTACAAGGGACTCAGGCGACGTGTGCTTGGGATTGTGATGCAAAATCCAGCTATATTAGAGGATGAAATTATTCACCGGATGGATGTATTGAATCCCCAGAGCTGTAGAAAATTGTTGGAGTTGATGGTTCTGGATAACCATCTTCATGTCAGGAAGATGCATCAAACTACCTGCGCTGGGCCCCCTCCAATTTTAGGTGCCCTCCTTGGAAGCAGCTATAAACCATCAAAGCTAGTTTGCCGTGAGCACTACTTTGCAAATCCCATGAGTACATCCTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1878

Amino Acids

210.79

Weight (kDa)

6.97

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7599 PF24538 48 - 128 4.4e-35 Domain of unknown function (DUF7599)
DUF7646 PF24657 149 - 232 6.9e-38 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 365 - 474 6.5e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 560 - 739 5.5e-83 Domain of unknown function (DUF7647)
DUF7645 PF24655 742 - 797 7.8e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2910
AarI CACCTGC 1 cut(s) 2881
AasI GACNNNNNNGTC 1 cut(s) 5029
AatII GACGTC 1 cut(s) 2983
Acc36I ACCTGC 2 cut(s) 2881, 5528
Acc65I GGTACC 1 cut(s) 5330
AccB1I GGYRCC 2 cut(s) 5330, 5546
AccBSI CCGCTC 2 cut(s) 144, 2475
AccI GTMKAC 4 cut(s) 297, 2977, 3960, 5349
AccII CGCG 1 cut(s) 348
AccIII TCCGGA 2 cut(s) 4810, 5033
AcoI YGGCCR 2 cut(s) 357, 3729
AcyI GRCGYC 2 cut(s) 2920, 2980
AflII CTTAAG 1 cut(s) 2531
AflIII ACRYGT 4 cut(s) 346, 946, 3287, 5368
AjiI CACGTC 2 cut(s) 3290, 5369
AjnI CCWGG 5 cut(s) 359, 1082, 2716, 2883, 4900
AjuI GAANNNNNNNTTGG 6 cut(s) 1473, 1505, 3289, 3321, 4593, 4625
AleI CACNNNNGTG 2 cut(s) 1544, 1874
AloI GAACNNNNNNTCC 2 cut(s) 2478, 2510
Alw21I GWGCWC 5 cut(s) 1154, 1754, 1976, 5208, 5598
Alw26I GTCTC 6 cut(s) 1096, 1371, 1733, 2357, 2388, 4534
Alw44I GTGCAC 1 cut(s) 1750
AlwNI CAGNNNCTG 5 cut(s) 1760, 2012, 3080, 3404, 4532
Ama87I CYCGRG 2 cut(s) 443, 4516
Aor13HI TCCGGA 2 cut(s) 4810, 5033
AoxI GGCC 8 cut(s) 55, 283, 357, 1387, 1855, 2265, 3729, 5528
ApaI GGGCCC 1 cut(s) 5532
ApaLI GTGCAC 1 cut(s) 1750
AseI ATTAAT 1 cut(s) 1245
Asp700I GAANNNNTTC 4 cut(s) 2025, 2283, 3301, 5418
Asp718I GGTACC 1 cut(s) 5330
AspLEI GCGC 4 cut(s) 478, 1562, 2054, 5525
AsuC2I CCSGG 5 cut(s) 1344, 3383, 3809, 4517, 4518
AsuII TTCGAA 2 cut(s) 2455, 3980
AvaI CYCGRG 2 cut(s) 443, 4516
AvaII GGWCC 6 cut(s) 78, 363, 1496, 2003, 2689, 3772
BaeGI GKGCMC 5 cut(s) 583, 1754, 1812, 5532, 5551
BalI TGGCCA 2 cut(s) 359, 3731
BamHI GGATCC 1 cut(s) 2300
BanI GGYRCC 2 cut(s) 5330, 5546
BanII GRGCYC 2 cut(s) 5208, 5532
BbsI GAAGAC 2 cut(s) 3225, 4590
Bbv12I GWGCWC 5 cut(s) 1154, 1754, 1976, 5208, 5598
BbvCI CCTCAGC 2 cut(s) 618, 5259
BceAI ACGGC 6 cut(s) 70, 1181, 1694, 1842, 4079, 5574
BcgI CGANNNNNNTGC 2 cut(s) 2879, 2913
BciT130I CCWGG 5 cut(s) 361, 1084, 2718, 2885, 4902
BciVI GTATCC 1 cut(s) 1632
BclI TGATCA 2 cut(s) 1330, 3628
BcnI CCSGG 5 cut(s) 1344, 3383, 3809, 4517, 4518
BcoDI GTCTC 6 cut(s) 1096, 1371, 1733, 2357, 2388, 4534
BfmI CTRYAG 8 cut(s) 1062, 1872, 3588, 3987, 4476, 4554, 5233, 5452
BfoI RGCGCY 1 cut(s) 1563
BfrI CTTAAG 1 cut(s) 2531
BfuAI ACCTGC 2 cut(s) 2881, 5528
BfuI GTATCC 1 cut(s) 1632
BglII AGATCT 3 cut(s) 2996, 3781, 3890
BlpI GCTNAGC 2 cut(s) 840, 1710
BmcAI AGTACT 3 cut(s) 2230, 3920, 4699
Bme18I GGWCC 6 cut(s) 78, 363, 1496, 2003, 2689, 3772
BmeT110I CYCGRG 2 cut(s) 443, 4516
BmgBI CACGTC 2 cut(s) 3290, 5369
BmiI GGNNCC 9 cut(s) 80, 513, 2302, 2550, 2691, 5332, 5530, 5531, 5548
BmrI ACTGGG 1 cut(s) 3180
BmuI ACTGGG 1 cut(s) 3180
BpiI GAAGAC 2 cut(s) 3225, 4590
BplI GAGNNNNNCTC 2 cut(s) 1891, 1923
BpmI CTGGAG 2 cut(s) 2906, 5121
Bpu10I CCTNAGC 2 cut(s) 618, 5259
Bpu1102I GCTNAGC 2 cut(s) 840, 1710
Bpu14I TTCGAA 2 cut(s) 2455, 3980
BpuEI CTTGAG 5 cut(s) 1138, 1506, 3722, 4033, 4770
BpuMI CCSGG 5 cut(s) 1344, 3383, 3809, 4517, 4518
Bsa29I ATCGAT 1 cut(s) 1207
BsaBI GATNNNNATC 5 cut(s) 596, 2584, 3633, 4257, 5487
BsaHI GRCGYC 2 cut(s) 2920, 2980
BsaWI WCCGGW 4 cut(s) 3774, 4810, 5033, 5424
BsaXI ACNNNNNCTCC 4 cut(s) 305, 335, 3930, 3960
Bse118I RCCGGY 1 cut(s) 697
Bse1I ACTGG 7 cut(s) 2006, 2273, 2410, 3175, 3765, 4797, 5131
Bse3DI GCAATG 1 cut(s) 708
Bse8I GATNNNNATC 5 cut(s) 596, 2584, 3633, 4257, 5487
BseAI TCCGGA 2 cut(s) 4810, 5033
BseBI CCWGG 5 cut(s) 361, 1084, 2718, 2885, 4902
BseCI ATCGAT 1 cut(s) 1207
BseJI GATNNNNATC 5 cut(s) 596, 2584, 3633, 4257, 5487
BseMI GCAATG 1 cut(s) 708
BseNI ACTGG 7 cut(s) 2006, 2273, 2410, 3175, 3765, 4797, 5131
BseRI GAGGAG 6 cut(s) 91, 94, 635, 3329, 3719, 5265
BseSI GKGCMC 5 cut(s) 583, 1754, 1812, 5532, 5551
BseYI CCCAGC 5 cut(s) 400, 582, 3412, 4850, 5525
BsgI GTGCAG 1 cut(s) 1868
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 1780
BshFI GGCC 8 cut(s) 57, 285, 359, 1389, 1857, 2267, 3731, 5530
BshNI GGYRCC 2 cut(s) 5330, 5546
BshVI ATCGAT 1 cut(s) 1207
BsiEI CGRYCG 1 cut(s) 1780
BsiHKAI GWGCWC 5 cut(s) 1154, 1754, 1976, 5208, 5598
BsiHKCI CYCGRG 2 cut(s) 443, 4516
BsmAI GTCTC 6 cut(s) 1096, 1371, 1733, 2357, 2388, 4534
BsmBI CGTCTC 1 cut(s) 1733
BsmI GAATGC 1 cut(s) 3988
BsnI GGCC 8 cut(s) 57, 285, 359, 1389, 1857, 2267, 3731, 5530
BsoBI CYCGRG 2 cut(s) 443, 4516
Bsp119I TTCGAA 2 cut(s) 2455, 3980
Bsp120I GGGCCC 1 cut(s) 5528
Bsp1286I GDGCHC 9 cut(s) 583, 1154, 1754, 1812, 1976, 5208, 5532, 5551, 5598
Bsp13I TCCGGA 2 cut(s) 4810, 5033
Bsp1407I TGTACA 2 cut(s) 943, 1458
Bsp1720I GCTNAGC 2 cut(s) 840, 1710
Bsp19I CCATGG 3 cut(s) 354, 3662, 5312
BspANI GGCC 8 cut(s) 57, 285, 359, 1389, 1857, 2267, 3731, 5530
BspDI ATCGAT 1 cut(s) 1207
BspEI TCCGGA 2 cut(s) 4810, 5033
BspFNI CGCG 1 cut(s) 348
BspHI TCATGA 3 cut(s) 1930, 2731, 3601
BspLI GGNNCC 9 cut(s) 80, 513, 2302, 2550, 2691, 5332, 5530, 5531, 5548
BspMAI CTGCAG 2 cut(s) 1066, 4480
BspMI ACCTGC 2 cut(s) 2881, 5528
BspQI GCTCTTC 1 cut(s) 3654
BspT104I TTCGAA 2 cut(s) 2455, 3980
BspT107I GGYRCC 2 cut(s) 5330, 5546
BspTI CTTAAG 1 cut(s) 2531
BsrBI CCGCTC 2 cut(s) 144, 2475
BsrDI GCAATG 1 cut(s) 708
BsrFI RCCGGY 1 cut(s) 697
BsrGI TGTACA 2 cut(s) 943, 1458
BsrI ACTGG 7 cut(s) 2006, 2273, 2410, 3175, 3765, 4797, 5131
BssAI RCCGGY 1 cut(s) 697
BssNI GRCGYC 2 cut(s) 2920, 2980
BssT1I CCWWGG 6 cut(s) 354, 3225, 3662, 4733, 5312, 5554
Bst2UI CCWGG 5 cut(s) 361, 1084, 2718, 2885, 4902
Bst6I CTCTTC 5 cut(s) 603, 2987, 3654, 4208, 5187
BstACI GRCGYC 2 cut(s) 2920, 2980
BstAFI CTTAAG 1 cut(s) 2531
BstAPI GCANNNNNTGC 1 cut(s) 3833
BstAUI TGTACA 2 cut(s) 943, 1458
BstBI TTCGAA 2 cut(s) 2455, 3980
BstDSI CCRYGG 6 cut(s) 354, 1197, 1641, 3087, 3662, 5312
BstENI CCTNNNNNAGG 3 cut(s) 2888, 3704, 4737
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 1563
BstHHI GCGC 4 cut(s) 478, 1562, 2054, 5525
BstMAI GTCTC 6 cut(s) 1096, 1371, 1733, 2357, 2388, 4534
BstMCI CGRYCG 1 cut(s) 1780
BstNI CCWGG 5 cut(s) 361, 1084, 2718, 2885, 4902
BstNSI RCATGY 2 cut(s) 950, 5302
BstSFI CTRYAG 8 cut(s) 1062, 1872, 3588, 3987, 4476, 4554, 5233, 5452
BstSLI GKGCMC 5 cut(s) 583, 1754, 1812, 5532, 5551
BstUI CGCG 1 cut(s) 348
BstV2I GAAGAC 2 cut(s) 3225, 4590
BstXI CCANNNNNNTGG 3 cut(s) 1876, 2405, 5064
Bsu15I ATCGAT 1 cut(s) 1207
BsuI GTATCC 1 cut(s) 1632
BsuRI GGCC 8 cut(s) 57, 285, 359, 1389, 1857, 2267, 3731, 5530
BsuTUI ATCGAT 1 cut(s) 1207
BtgI CCRYGG 6 cut(s) 354, 1197, 1641, 3087, 3662, 5312
BtrI CACGTC 2 cut(s) 3290, 5369
BtsI GCAGTG 3 cut(s) 717, 1810, 4833
BveI ACCTGC 2 cut(s) 2881, 5528
CaiI CAGNNNCTG 5 cut(s) 1760, 2012, 3080, 3404, 4532
CciI TCATGA 3 cut(s) 1930, 2731, 3601
CfoI GCGC 4 cut(s) 478, 1562, 2054, 5525
Cfr10I RCCGGY 1 cut(s) 697
Cfr9I CCCGGG 1 cut(s) 4516
ClaI ATCGAT 1 cut(s) 1207
CseI GACGC 4 cut(s) 337, 869, 1822, 2909
CspCI CAANNNNNGTGG 2 cut(s) 2833, 2868
DraI TTTAAA 1 cut(s) 4822
DrdI GACNNNNNNGTC 1 cut(s) 5029
DseDI GACNNNNNNGTC 1 cut(s) 5029
EaeI YGGCCR 2 cut(s) 357, 3729
Eam1104I CTCTTC 5 cut(s) 603, 2987, 3654, 4208, 5187
EarI CTCTTC 5 cut(s) 603, 2987, 3654, 4208, 5187
EciI GGCGGA 1 cut(s) 10
Ecl136II GAGCTC 1 cut(s) 5206
Eco130I CCWWGG 6 cut(s) 354, 3225, 3662, 4733, 5312, 5554
Eco24I GRGCYC 2 cut(s) 5208, 5532
Eco32I GATATC 1 cut(s) 4423
Eco47I GGWCC 6 cut(s) 78, 363, 1496, 2003, 2689, 3772
Eco53kI GAGCTC 1 cut(s) 5206
Eco88I CYCGRG 2 cut(s) 443, 4516
EcoICRI GAGCTC 1 cut(s) 5206
EcoNI CCTNNNNNAGG 3 cut(s) 2888, 3704, 4737
EcoO109I RGGNCCY 1 cut(s) 5529
EcoRI GAATTC 1 cut(s) 4547
EcoRII CCWGG 5 cut(s) 359, 1082, 2716, 2883, 4900
EcoRV GATATC 1 cut(s) 4423
EcoT14I CCWWGG 6 cut(s) 354, 3225, 3662, 4733, 5312, 5554
EcoT22I ATGCAT 5 cut(s) 1252, 1939, 2524, 5300, 5511
EcoT38I GRGCYC 2 cut(s) 5208, 5532
ErhI CCWWGG 6 cut(s) 354, 3225, 3662, 4733, 5312, 5554
Esp3I CGTCTC 1 cut(s) 1733
FalI AAGNNNNNCTT 4 cut(s) 444, 476, 3289, 3321
FauI CCCGC 4 cut(s) 89, 202, 1142, 1980
FauNDI CATATG 2 cut(s) 2518, 3841
FbaI TGATCA 2 cut(s) 1330, 3628
FblI GTMKAC 4 cut(s) 297, 2977, 3960, 5349
FriOI GRGCYC 2 cut(s) 5208, 5532
GlaI GCGC 4 cut(s) 477, 1561, 2053, 5524
GsaI CCCAGC 5 cut(s) 404, 586, 3416, 4854, 5529
GsuI CTGGAG 2 cut(s) 2906, 5121
HaeII RGCGCY 1 cut(s) 1563
HaeIII GGCC 8 cut(s) 57, 285, 359, 1389, 1857, 2267, 3731, 5530
HgaI GACGC 4 cut(s) 337, 869, 1822, 2909
HhaI GCGC 4 cut(s) 478, 1562, 2054, 5525
Hin1I GRCGYC 2 cut(s) 2920, 2980
Hin6I GCGC 4 cut(s) 476, 1560, 2052, 5523
HinP1I GCGC 4 cut(s) 476, 1560, 2052, 5523
HincII GTYRAC 4 cut(s) 298, 307, 2924, 4622
HindII GTYRAC 4 cut(s) 298, 307, 2924, 4622
HindIII AAGCTT 3 cut(s) 2834, 3926, 4001
Hpy99I CGWCG 8 cut(s) 56, 254, 260, 353, 2442, 2985, 4503, 5370
HpyCH4IV ACGT 8 cut(s) 978, 2788, 2974, 2980, 3139, 3289, 4411, 5368
HpySE526I ACGT 8 cut(s) 978, 2788, 2974, 2980, 3139, 3289, 4411, 5368
Hsp92I GRCGYC 2 cut(s) 2920, 2980
HspAI GCGC 4 cut(s) 476, 1560, 2052, 5523
Kpn2I TCCGGA 2 cut(s) 4810, 5033
KpnI GGTACC 1 cut(s) 5334
Ksp22I TGATCA 2 cut(s) 1330, 3628
LguI GCTCTTC 1 cut(s) 3654
LmnI GCTCC 6 cut(s) 149, 397, 511, 2887, 3841, 5140
MaeII ACGT 8 cut(s) 978, 2788, 2974, 2980, 3139, 3289, 4411, 5368
MbiI CCGCTC 2 cut(s) 144, 2475
MfeI CAATTG 1 cut(s) 3157
MhlI GDGCHC 9 cut(s) 583, 1154, 1754, 1812, 1976, 5208, 5532, 5551, 5598
MlsI TGGCCA 2 cut(s) 359, 3731
MluI ACGCGT 1 cut(s) 346
MluNI TGGCCA 2 cut(s) 359, 3731
MmeI TCCRAC 8 cut(s) 336, 352, 367, 941, 3736, 4223, 4624, 5445
Mox20I TGGCCA 2 cut(s) 359, 3731
Mph1103I ATGCAT 5 cut(s) 1252, 1939, 2524, 5300, 5511
MroI TCCGGA 2 cut(s) 4810, 5033
MroXI GAANNNNTTC 4 cut(s) 2025, 2283, 3301, 5418
MscI TGGCCA 2 cut(s) 359, 3731
Msp20I TGGCCA 2 cut(s) 359, 3731
MspA1I CMGCKG 4 cut(s) 1580, 2780, 3052, 3479
MspCI CTTAAG 1 cut(s) 2531
MunI CAATTG 1 cut(s) 3157
Mva1269I GAATGC 1 cut(s) 3988
MvaI CCWGG 5 cut(s) 361, 1084, 2718, 2885, 4902
MvnI CGCG 1 cut(s) 348
NciI CCSGG 5 cut(s) 1344, 3383, 3809, 4517, 4518
NcoI CCATGG 3 cut(s) 354, 3662, 5312
NdeI CATATG 2 cut(s) 2518, 3841
NlaIV GGNNCC 9 cut(s) 80, 513, 2302, 2550, 2691, 5332, 5530, 5531, 5548
NmeAIII GCCGAG 2 cut(s) 253, 540
NmuCI GTSAC 6 cut(s) 532, 637, 1843, 2465, 3118, 3546
NsiI ATGCAT 5 cut(s) 1252, 1939, 2524, 5300, 5511
NspI RCATGY 2 cut(s) 950, 5302
NspV TTCGAA 2 cut(s) 2455, 3980
OliI CACNNNNGTG 2 cut(s) 1544, 1874
PaeI GCATGC 1 cut(s) 5302
PaeR7I CTCGAG 1 cut(s) 443
PagI TCATGA 3 cut(s) 1930, 2731, 3601
PaqCI CACCTGC 1 cut(s) 2881
PciI ACATGT 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 3654
PcsI WCGNNNNNNNCGW 3 cut(s) 195, 255, 2980
PctI GAATGC 1 cut(s) 3988
PdmI GAANNNNTTC 4 cut(s) 2025, 2283, 3301, 5418
PflFI GACNNNGTC 1 cut(s) 302
PscI ACATGT 1 cut(s) 946
PshBI ATTAAT 1 cut(s) 1245
PsiI TTATAA 1 cut(s) 2910
Psp124BI GAGCTC 1 cut(s) 5208
Psp6I CCWGG 5 cut(s) 359, 1082, 2716, 2883, 4900
PspFI CCCAGC 5 cut(s) 400, 582, 3412, 4850, 5525
PspGI CCWGG 5 cut(s) 359, 1082, 2716, 2883, 4900
PspN4I GGNNCC 9 cut(s) 80, 513, 2302, 2550, 2691, 5332, 5530, 5531, 5548
PspOMI GGGCCC 1 cut(s) 5528
PstI CTGCAG 2 cut(s) 1066, 4480
PstNI CAGNNNCTG 5 cut(s) 1760, 2012, 3080, 3404, 4532
PsyI GACNNNGTC 1 cut(s) 302
PvuII CAGCTG 4 cut(s) 1580, 2780, 3052, 3479
SacI GAGCTC 1 cut(s) 5208
SalI GTCGAC 1 cut(s) 296
SapI GCTCTTC 1 cut(s) 3654
ScaI AGTACT 3 cut(s) 2230, 3920, 4699
SduI GDGCHC 9 cut(s) 583, 1154, 1754, 1812, 1976, 5208, 5532, 5551, 5598
SfcI CTRYAG 8 cut(s) 1062, 1872, 3588, 3987, 4476, 4554, 5233, 5452
Sfr274I CTCGAG 1 cut(s) 443
SfuI TTCGAA 2 cut(s) 2455, 3980
SinI GGWCC 6 cut(s) 78, 363, 1496, 2003, 2689, 3772
SlaI CTCGAG 1 cut(s) 443
SmaI CCCGGG 1 cut(s) 4518
SmlI CTYRAG 7 cut(s) 443, 1153, 1521, 2531, 3701, 4012, 4785
SmoI CTYRAG 7 cut(s) 443, 1153, 1521, 2531, 3701, 4012, 4785
SphI GCATGC 1 cut(s) 5302
SspI AATATT 1 cut(s) 817
SstI GAGCTC 1 cut(s) 5208
StyI CCWWGG 6 cut(s) 354, 3225, 3662, 4733, 5312, 5554
TaiI ACGT 8 cut(s) 981, 2791, 2977, 2983, 3142, 3292, 4414, 5371
TaqII GACCGA 1 cut(s) 1794
TauI GCSGC 2 cut(s) 227, 2920
TseFI GTSAC 6 cut(s) 532, 637, 1843, 2465, 3118, 3546
Tsp45I GTSAC 6 cut(s) 532, 637, 1843, 2465, 3118, 3546
TspGWI ACGGA 6 cut(s) 136, 292, 313, 1757, 3060, 4796
TspMI CCCGGG 1 cut(s) 4516
Tth111I GACNNNGTC 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 2531
VneI GTGCAC 1 cut(s) 1750
VpaK11BI GGWCC 6 cut(s) 78, 363, 1496, 2003, 2689, 3772
VspI ATTAAT 1 cut(s) 1245
XagI CCTNNNNNAGG 3 cut(s) 2888, 3704, 4737
XbaI TCTAGA 2 cut(s) 3163, 5273
XceI RCATGY 2 cut(s) 950, 5302
XhoI CTCGAG 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 4516
XmiI GTMKAC 4 cut(s) 297, 2977, 3960, 5349
XmnI GAANNNNTTC 4 cut(s) 2025, 2283, 3301, 5418
ZraI GACGTC 1 cut(s) 2981
ZrmI AGTACT 3 cut(s) 2230, 3920, 4699
Zsp2I ATGCAT 5 cut(s) 1252, 1939, 2524, 5300, 5511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.