Rw2G036260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004044
Physical Location & Seq
Forward (+)
59561740 .. 59572835
11096 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036260.1

Sequence Viewer

Length: 5628 bp
ATGGACTCCATTCTAAACTCAGCGATCGAAGAAATCTGTTCGTACCTCCAAAACGGCCTCTCTCTCCAGACCCTCTGGTCCCGACTCTCCTCCTCCTCCAACCTCGACCTCTCTCCCACCCTCAAACAATCCCTATGGGACGCCCTTCGCTCCGTCCCCACTCTCAAATTCCGCGCCCAAAATGACCGTTACGCCCCCACCGACCCTTCCATTCACTCCTTCCAAGACGCCGAGAACCTCAATTTGAAGCTCGTCGCCGATGAGGCCCTCCGGAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAATGCCAACCTGTCTGCCGTTCAACGCCACACTCTCGAACGCGTCGTCATGGCTAGGGGGAATGGGATTACACAAACTCAGCTTGCTAAGGAGCTTGGCATTGAGGGGAGAAACTTTCATTATGCTGTGAAGAACCTCGAATGTCAAGGGTTACTGGTAAGGCAATCTGCGCTGTTAAGGACAAAAGAAGCTGGTGATGAGGGGGAGCCGAGGAACAATCCGAGTGTGACAACGACCAATATGTTGTACTTGTATCGACATGCAAAACATTTAAGCGCGCAACAAAAGATTGAGATCACAAAGGAAGAGAGGGCTAAGGAGAGTTTTGTAAATGAAAAAGAAAGCCCTGCAAGTGGAGATGGTTTTGCTGGAAATGTGCTCGTAAAGGACTATCTACCGGCAATGAAAGCAGTCTGTAATAAACTTGAAGAAGCCAATGGCAAGGTTCTTGTCGTTTCGGATATTAAGAAGGAACTTGGCTACAGTAAAACATCAGGGCATAGAGCTTGGAGAAATATTTGTCAGCGGCTGAAAGCTGCTCATCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGGAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAACGAAAGTTGAGCCAAGAGAAGTTGTACGTGTAGATGAGGATTTTGTTGAAGAACAACAAGTGAAATTCGGAAAGAAATGTAAAATCAGTGACCAGCTTGTAGAGCTTCCTATTGAGCATCAAATTTATGAATTAATAGATGCTGCAGGATCAGAGGGGTTGACCAGGAATGAGGTTATGGAGAGGCTTGGAATTGACAACAAGAAAAATTATACCCGCTTTGTTAGTATGTGCTCTAGATTTGATATGAGTCTGCAACCAGAAATGCACAAAAAAGCCGTGGCATATCGATTTCGGACATCTGGGAAACACAAGTCTGAATCAGCTAATGCATTTCTTCAAAAATCAAAAGATGCCAATGACAGTAAAATTTCCAGTCTAAATGTTGGGAGTGTAGATGCTCCGAGATCAGATCAATTTCAAACTGGCTCATTGTCAGATTGTTGGTCTCTGAACAGTGATACTGCTGGTGGCCCTGAAAATATGAATAACATAGAGACTAATACTGACCCTTCTGCTGGATCTCTTGGGCGTAATGAGTTTGACGATATGCCTGAAACTTCCCAGCAGTTACTTCTTGGACCAAAAGATACTACATCTGATTCTCAAGTTAGTTTAGTGAGCGCAGAAGTGGAACCAAATGGCGCCTTATCAGAAATACCAGCTGCTTTCTCAAAGCCACTTGCTAAAGGCTCAGATCCAAGGTATCCATGTATGTCTTTGACCGTGGATAACACTCGAAGGGAAAAGAGGATACTTGAAAGGCTAGAGGGTGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGACAAGTGCACAGCAACTGACAGGAAAACAATTGACCGAATTTTACAAAAACTTCAACAGTTAGGGCACTGCAAGTGTATAGATATTAGCGTCCCTGTCGTCACAAATTTGGGCCGTACCCGAACCACTGTAGTGGTTCTGCACCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCTTGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAGTGGATCAGTTCCTGTACTAGAAGATGTTCAGAGAACTCAGACCCATGTTAGTGCACAGCGACAAACACTGAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTACTGCATAGTTTCCTGTGGGATTACCTGTATGGTTCGTCCGGCTCTAATGATGCTTTATCATTTGGAAAAGATGTTATTGAGCCAAGAGATCCTCACAGTACTAGAAAATTGTTTTCTCTAGAAGCAACAATGAAGGCTATTCCAGTTGAGTTGTTTCTACAAGTTGCGGGATCCACCAAAAACTATGAGGATATGATTGAGAAATGTAAGAGGGGTTTATGTCTCTCTGATCTCTCTCGTGAAGAATACAAGTCTCTGATGGATACCCATGCAACTGGAAGGCTCTCATTGGTGATTGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGATCACCACTCAGAAAATGGATTCCAAGTTCCTCCTCCCATGATTTCTGCATATGCATTGGAACTTAAGCCTTATGTAGAGGAACCTGTTTCAAAAGATGCGATATCTTTGAGTTTTGGATCACGTGATCTTCGTCCACGAATCAGACATGATTTTTACCTATCAAGCAGAGAAGCTCTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCGGACCCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAGCGTGATGAGCTCTTAAAGCGTGTTGTGAAGGATGATCCAAGTGAAAAGCTTTCATTCAAAGAGTGTGGAAAAATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACACGGATGAAGTTCAATCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATTATGAGGTAACTGGACAGCTGGAAGAGCAGATACATCCCACAGTATCTGATACTGTGGAACAATTAGAAGAACTAAATCTCTTGGTCACTTCTTATGAGCATGATTCACATTTGCAAGCATTGGACAATTGTCTAGAAACTGGGCAGGAACCTGAACCAAATGAAGATAATGAGGGGTGCCATTCAATTATTACCAAGGGTTCCTTTTCAAAGTTGAAGCCACTACGTCCTAAAAGAAACAAGTTGCACTCAACACGTCAACGAAGGTTTTCATGGACAGAGGAGGCTGATAGGCAGTTGATTATCCAATATGTAAGACATCGTGCAACTCTTGGGGCAAAGATTCACCGGATAAATTGGGCTTCAGTTACTGACCTCCCAGCACCTCCTATTGCCTGCATGAAAAGAATGGCATCTCTGAAAAGTAACAGAAAGTTCAGATTAGCTGTAATGAGGCTTTGTAACATTCTCAGTGAACGATATGCAAAGATCTTGGAAAAGACTCAGAACAGGTCACTGAACAAAGATGATTGCAATCTGCTTTTGCGAGATTCAATAGGTGAAGGTCATGACAGCAAACTTCCCAATATCAGTGATCATAATCCAGAGACAGTTCTTCAGGAAGAACCATGGGATGATTTTGATGACGCTTATGTTAAAAAGTTGGCCAAATTTGATGCCTCCAAAAGAGTTGGATCTACCGGTGAGGACTGGTCGGATCTTAATACAAGTGAATGTGATCCCCACGAATCTGAATTAATTGTATCAACTGCTCCATATGAAGATGTTCAGAATCATGGTGGCAGAGAAAAAATTTCGGCACGAAGATCTTGCTATCAACACCTTAATGAAAAATACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACACAAGTCTACAAATCATTAGCTGTTTCTAATGCTGTAGAGCTGTTTAAGCTTGTTTTCCTCAGTACCTCAACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCATTCTACGGCGGTACTCAGAGTGTGATCTATTCGCAGCTTTCAACTATCTTAGAGATAAAAAGTTCATGGTTGGAGGTAATGGCAGTCAAAAATTTTCTCTCTCACAACAGTTCTTGCATAATACTTCCGCATCACCATTTCCAATTAATTCTGCGAAGAGAGCTACTAAATTCGCCCATTGGCTCTACGAAAAAGATAAAGATCTTATGGAAGGGGGGATCGATCTCTCCACAGATTTACAGTGTGGGGACATATTCCATTTGTTTGCTCTAGTTTCTTCAGGTGAACTGTCAATTTCTCCATGCTTGCCAGATGAAGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCAGATATCAATGAACTTTTAGATGATGAAAAAACTAAAAAGCTGAAATCATTTGTGGCTGCAGAAGGTGAAATCATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCACGGTATCTGTAAGTCGTGCAGAGTTTTCTATAGCCAATTCTATAGATTTGTTCAAGGAAGACGCTCCTATTGGTGACAAACATTTTGGGGGAAATCATCAACTAGAATGTACATCTGATCAGAGTAGCTTATCACATTCTGACTGTATGAAAGAAATCTTCAATTCACTCAGTACTGTCCCTGTATTGGAGATTGGTTGCGAGTCACCTTGGGAAGGTATGGTGGCTTATGCCGAACATTTGTTGCCATTACATCCTGCTCAAGATCAATCAAGTCCCATCCAGCCTGAGGTCTTTAGGACTGTTTACATTGCTATTCAGAAAGCTGGCGATCAAGGTCTGGGCATAGAGGAAGTTTCCCGAATAATGAATATCCCAGGAGAAAAGATGACTGATCTTATCATTGATGTGCTTCAGACATTTGAACGAGTCTTAAAGGTCAATGCTTATGACTCTATTCGTGTTGTGGATTCTTTGTATCGTGGCAAGTACTTTATGACTTCAGTGTCTGGAATTAATCAAAAACTCGAACCACCTTCCTGGAGAAAGCCACTAGGCGATGACGGCCATATACTCTTCCATTCAGAAAATCGTGATAATGGTGCTGCTCCTTCAGAGAGGGAGATAAATGCTGATGTCCATAAATTGACAATTCTTAATTTTCCTGAAGAGGTTGATGAGCCCTCATATGTAAAACAAATTAGCAGTGTTCCTGAAAGCTATAGGGAAGGAAAAGGAGGAGATGCTGAGGATGAATCTTCTAGGTCCTCTAATGATAGACTATGCATGCCTATATTTCCATGGATAAATGGTGATGGGACCACTAACAAGATTGTCTACAAGGGACTCAGGCGACGTGTGCTTGGGGTTGTGATGCAAAATCCAGCTATATTAGAGGATGAAATTATTCGCCGGATGGATGTATTGAATCCCCAGAGCTGTAGAAAATTGTTGGAATTGATGGTTTTGGATAACCATCTTCATGTGAGGAAGATGCATCAAACTGCGTGCATTGGGCCCCCACCAATTTTAGGTACCCTCCTTGGAAGCAGCTTCAAACCGTCAAAGCTGGTTTGTCGCGAGCACTATTTTGCAAATCCCATGAGTACATTCTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1875

Amino Acids

211.35

Weight (kDa)

6.44

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 98 1.1e-33 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 109 - 192 1.2e-12 B-block binding subunit of TFIIIC
DUF7599 PF24538 232 - 314 7.2e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 333 - 417 1.1e-37 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 550 - 659 3.1e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 745 - 924 1.1e-82 Domain of unknown function (DUF7647)
DUF7645 PF24655 927 - 982 8.7e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2922
AarI CACCTGC 1 cut(s) 2893
AasI GACNNNNNNGTC 2 cut(s) 76, 5011
AatII GACGTC 1 cut(s) 2995
Acc36I ACCTGC 1 cut(s) 2893
Acc65I GGTACC 1 cut(s) 5540
AccB1I GGYRCC 3 cut(s) 1571, 3222, 5540
AccI GTMKAC 4 cut(s) 300, 2989, 3942, 5343
AccII CGCG 4 cut(s) 174, 351, 587, 5586
AccIII TCCGGA 1 cut(s) 270
AciI CCGC 9 cut(s) 172, 835, 1144, 1999, 2309, 2699, 2929, 4048, 4167
AcoI YGGCCR 2 cut(s) 3711, 5071
AcuI CTGAAG 8 cut(s) 3393, 3647, 4302, 4392, 4904, 4992, 5103, 5193
AcvI CACGTG 1 cut(s) 2603
AcyI GRCGYC 5 cut(s) 141, 228, 1572, 2932, 2992
AfiI CCNNNNNNNGG 9 cut(s) 662, 1445, 2760, 2902, 4355, 4693, 4721, 4795, 5537
AflII CTTAAG 1 cut(s) 2543
AflIII ACRYGT 4 cut(s) 349, 955, 3299, 5362
AgeI ACCGGT 1 cut(s) 3746
AjiI CACGTC 2 cut(s) 3302, 5363
AjnI CCWGG 5 cut(s) 1091, 2728, 2895, 4882, 5045
AleI CACNNNNGTG 1 cut(s) 1886
AloI GAACNNNNNNTCC 2 cut(s) 2490, 2522
Alw21I GWGCWC 7 cut(s) 690, 1163, 1766, 1988, 2068, 2811, 5592
Alw26I GTCTC 6 cut(s) 1380, 1418, 1745, 2369, 2400, 3647
Alw44I GTGCAC 2 cut(s) 1762, 2064
AlwNI CAGNNNCTG 6 cut(s) 838, 1772, 2024, 3092, 3416, 5015
Ama87I CYCGRG 1 cut(s) 4498
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 8 cut(s) 55, 264, 286, 1399, 1867, 3711, 5071, 5522
ApaI GGGCCC 1 cut(s) 5526
ApaLI GTGCAC 2 cut(s) 1762, 2064
ArsI GACNNNNNNTTYG 4 cut(s) 339, 371, 4574, 4606
AseI ATTAAT 4 cut(s) 1061, 3803, 4185, 5022
AsiGI ACCGGT 1 cut(s) 3746
Asp700I GAANNNNTTC 5 cut(s) 278, 2037, 3313, 3831, 5412
Asp718I GGTACC 1 cut(s) 5540
AspLEI GCGC 6 cut(s) 176, 481, 587, 589, 1553, 1574
AsuC2I CCSGG 2 cut(s) 4499, 4500
AsuII TTCGAA 1 cut(s) 2467
AvaI CYCGRG 1 cut(s) 4498
AvaII GGWCC 5 cut(s) 78, 1508, 2701, 5271, 5325
AxyI CCTNAGG 1 cut(s) 4794
BaeGI GKGCMC 4 cut(s) 1766, 1824, 2068, 5526
BalI TGGCCA 1 cut(s) 3713
BamHI GGATCC 1 cut(s) 2312
BanI GGYRCC 3 cut(s) 1571, 3222, 5540
BanII GRGCYC 3 cut(s) 2811, 5190, 5526
BarI GAAGNNNNNNTAC 2 cut(s) 3060, 3092
BauI CACGAG 1 cut(s) 2379
BbrPI CACGTG 1 cut(s) 2603
BbsI GAAGAC 1 cut(s) 4572
Bbv12I GWGCWC 7 cut(s) 690, 1163, 1766, 1988, 2068, 2811, 5592
BbvCI CCTCAGC 1 cut(s) 5253
BceAI ACGGC 6 cut(s) 70, 311, 1190, 1854, 4061, 5086
BcgI CGANNNNNNTGC 4 cut(s) 2891, 2925, 3858, 3892
BciT130I CCWGG 5 cut(s) 1093, 2730, 2897, 4884, 5047
BciVI GTATCC 3 cut(s) 1644, 1674, 2398
BclI TGATCA 3 cut(s) 2479, 3640, 4624
BcnI CCSGG 2 cut(s) 4499, 4500
BcoDI GTCTC 6 cut(s) 1380, 1418, 1745, 2369, 2400, 3647
BfmI CTRYAG 9 cut(s) 790, 1071, 1884, 3969, 4458, 4536, 4548, 5227, 5446
BfoI RGCGCY 1 cut(s) 1575
BfrI CTTAAG 1 cut(s) 2543
BfuAI ACCTGC 1 cut(s) 2893
BfuI GTATCC 3 cut(s) 1644, 1674, 2398
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 4 cut(s) 3008, 3534, 3872, 4240
BlpI GCTNAGC 2 cut(s) 1722, 2796
BmcAI AGTACT 3 cut(s) 2242, 4681, 4997
Bme1390I CCNGG 7 cut(s) 1093, 2730, 2897, 4499, 4500, 4884, 5047
Bme18I GGWCC 5 cut(s) 78, 1508, 2701, 5271, 5325
BmeT110I CYCGRG 1 cut(s) 4498
BmgBI CACGTC 2 cut(s) 3302, 5363
BmrFI CCNGG 7 cut(s) 1093, 2730, 2897, 4499, 4500, 4884, 5047
BmrI ACTGGG 1 cut(s) 3195
BmuI ACTGGG 1 cut(s) 3195
BoxI GACNNNNGTC 1 cut(s) 3174
BpiI GAAGAC 1 cut(s) 4572
BplI GAGNNNNNCTC 2 cut(s) 1903, 1935
BpmI CTGGAG 3 cut(s) 50, 2918, 5068
Bpu10I CCTNAGC 3 cut(s) 396, 624, 5253
Bpu1102I GCTNAGC 2 cut(s) 1722, 2796
Bpu14I TTCGAA 1 cut(s) 2467
BpuEI CTTGAG 2 cut(s) 1518, 4752
BpuMI CCSGG 2 cut(s) 4499, 4500
Bsa29I ATCGAT 2 cut(s) 1216, 4260
BsaAI YACGTR 2 cut(s) 956, 2603
BsaBI GATNNNNATC 5 cut(s) 602, 3579, 3645, 4239, 5481
BsaHI GRCGYC 5 cut(s) 141, 228, 1572, 2932, 2992
BsaI GGTCTC 1 cut(s) 1380
BsaWI WCCGGW 3 cut(s) 270, 3393, 3746
BsaXI ACNNNNNCTCC 2 cut(s) 3912, 3942
Bsc4I CCNNNNNNNGG 9 cut(s) 662, 1445, 2760, 2902, 4355, 4693, 4721, 4795, 5537
Bse118I RCCGGY 2 cut(s) 706, 3746
Bse1I ACTGG 8 cut(s) 468, 1302, 1357, 2285, 2422, 3061, 3190, 3761
Bse21I CCTNAGG 1 cut(s) 4794
Bse3DI GCAATG 2 cut(s) 717, 4815
Bse8I GATNNNNATC 5 cut(s) 602, 3579, 3645, 4239, 5481
BseAI TCCGGA 1 cut(s) 270
BseBI CCWGG 5 cut(s) 1093, 2730, 2897, 4884, 5047
BseCI ATCGAT 2 cut(s) 1216, 4260
BseJI GATNNNNATC 5 cut(s) 602, 3579, 3645, 4239, 5481
BseLI CCNNNNNNNGG 9 cut(s) 662, 1445, 2760, 2902, 4355, 4693, 4721, 4795, 5537
BseMI GCAATG 2 cut(s) 717, 4815
BseNI ACTGG 8 cut(s) 468, 1302, 1357, 2285, 2422, 3061, 3190, 3761
BsePI GCGCGC 1 cut(s) 585
BseRI GAGGAG 6 cut(s) 79, 82, 85, 2502, 3341, 5259
BseSI GKGCMC 4 cut(s) 1766, 1824, 2068, 5526
BseYI CCCAGC 2 cut(s) 1491, 3424
BsgI GTGCAG 2 cut(s) 1880, 4545
Bsh1236I CGCG 4 cut(s) 174, 351, 587, 5586
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 8 cut(s) 57, 266, 288, 1401, 1869, 3713, 5073, 5524
BshNI GGYRCC 3 cut(s) 1571, 3222, 5540
BshTI ACCGGT 1 cut(s) 3746
BshVI ATCGAT 2 cut(s) 1216, 4260
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 7 cut(s) 690, 1163, 1766, 1988, 2068, 2811, 5592
BsiHKCI CYCGRG 1 cut(s) 4498
BsiSI CCGG 7 cut(s) 271, 707, 2181, 3394, 3747, 4499, 5419
BslI CCNNNNNNNGG 9 cut(s) 662, 1445, 2760, 2902, 4355, 4693, 4721, 4795, 5537
BsmAI GTCTC 6 cut(s) 1380, 1418, 1745, 2369, 2400, 3647
BsmBI CGTCTC 1 cut(s) 1745
BsmI GAATGC 1 cut(s) 4038
BsnI GGCC 8 cut(s) 57, 266, 288, 1401, 1869, 3713, 5073, 5524
Bso31I GGTCTC 1 cut(s) 1380
BsoBI CYCGRG 1 cut(s) 4498
Bsp119I TTCGAA 1 cut(s) 2467
Bsp120I GGGCCC 1 cut(s) 5522
Bsp13I TCCGGA 1 cut(s) 270
Bsp1407I TGTACA 1 cut(s) 4616
Bsp1720I GCTNAGC 2 cut(s) 1722, 2796
Bsp19I CCATGG 2 cut(s) 3674, 5306
Bsp68I TCGCGA 1 cut(s) 5586
BspACI CCGC 9 cut(s) 172, 835, 1144, 1999, 2309, 2699, 2929, 4048, 4167
BspANI GGCC 8 cut(s) 57, 266, 288, 1401, 1869, 3713, 5073, 5524
BspDI ATCGAT 2 cut(s) 1216, 4260
BspEI TCCGGA 1 cut(s) 270
BspFNI CGCG 4 cut(s) 174, 351, 587, 5586
BspHI TCATGA 3 cut(s) 1942, 2743, 3613
BspMAI CTGCAG 2 cut(s) 1075, 4462
BspMI ACCTGC 1 cut(s) 2893
BspQI GCTCTTC 1 cut(s) 3063
BspT104I TTCGAA 1 cut(s) 2467
BspT107I GGYRCC 3 cut(s) 1571, 3222, 5540
BspTI CTTAAG 1 cut(s) 2543
BspTNI GGTCTC 1 cut(s) 1380
BsrDI GCAATG 2 cut(s) 717, 4815
BsrFI RCCGGY 2 cut(s) 706, 3746
BsrGI TGTACA 1 cut(s) 4616
BsrI ACTGG 8 cut(s) 468, 1302, 1357, 2285, 2422, 3061, 3190, 3761
BssAI RCCGGY 2 cut(s) 706, 3746
BssHII GCGCGC 1 cut(s) 585
BssNI GRCGYC 5 cut(s) 141, 228, 1572, 2932, 2992
BssSI CACGAG 1 cut(s) 2379
BssT1I CCWWGG 6 cut(s) 1628, 3240, 3674, 4715, 5306, 5548
Bst2BI CACGAG 1 cut(s) 2379
Bst2UI CCWGG 5 cut(s) 1093, 2730, 2897, 4884, 5047
Bst6I CTCTTC 6 cut(s) 609, 2999, 3063, 4190, 5087, 5169
BstACI GRCGYC 5 cut(s) 141, 228, 1572, 2932, 2992
BstAFI CTTAAG 1 cut(s) 2543
BstAUI TGTACA 1 cut(s) 4616
BstBAI YACGTR 2 cut(s) 956, 2603
BstBI TTCGAA 1 cut(s) 2467
BstDSI CCRYGG 4 cut(s) 1206, 1653, 3674, 5306
BstENI CCTNNNNNAGG 2 cut(s) 2900, 4719
BstFNI CGCG 4 cut(s) 174, 351, 587, 5586
BstH2I RGCGCY 1 cut(s) 1575
BstHHI GCGC 6 cut(s) 176, 481, 587, 589, 1553, 1574
BstMAI GTCTC 6 cut(s) 1380, 1418, 1745, 2369, 2400, 3647
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 5 cut(s) 1093, 2730, 2897, 4884, 5047
BstNSI RCATGY 2 cut(s) 572, 5296
BstPAI GACNNNNGTC 1 cut(s) 3174
BstSCI CCNGG 7 cut(s) 1091, 2728, 2895, 4497, 4498, 4882, 5045
BstSFI CTRYAG 9 cut(s) 790, 1071, 1884, 3969, 4458, 4536, 4548, 5227, 5446
BstSLI GKGCMC 4 cut(s) 1766, 1824, 2068, 5526
BstUI CGCG 4 cut(s) 174, 351, 587, 5586
BstV2I GAAGAC 1 cut(s) 4572
BstXI CCANNNNNNTGG 3 cut(s) 1888, 2417, 5046
Bsu15I ATCGAT 2 cut(s) 1216, 4260
Bsu36I CCTNAGG 1 cut(s) 4794
BsuI GTATCC 3 cut(s) 1644, 1674, 2398
BsuRI GGCC 8 cut(s) 57, 266, 288, 1401, 1869, 3713, 5073, 5524
BsuTUI ATCGAT 2 cut(s) 1216, 4260
BtgI CCRYGG 4 cut(s) 1206, 1653, 3674, 5306
BtgZI GCGATG 1 cut(s) 5079
BtrI CACGTC 2 cut(s) 3302, 5363
BtsI GCAGTG 2 cut(s) 1822, 5218
BtuMI TCGCGA 1 cut(s) 5586
BveI ACCTGC 1 cut(s) 2893
CaiI CAGNNNCTG 6 cut(s) 838, 1772, 2024, 3092, 3416, 5015
CciI TCATGA 3 cut(s) 1942, 2743, 3613
CfoI GCGC 6 cut(s) 176, 481, 587, 589, 1553, 1574
Cfr10I RCCGGY 2 cut(s) 706, 3746
Cfr9I CCCGGG 1 cut(s) 4498
ClaI ATCGAT 2 cut(s) 1216, 4260
CseI GACGC 8 cut(s) 149, 236, 340, 878, 1834, 2921, 3701, 4577
CspAI ACCGGT 1 cut(s) 3746
CspCI CAANNNNNGTGG 2 cut(s) 2845, 2880
DinI GGCGCC 1 cut(s) 1573
DrdI GACNNNNNNGTC 2 cut(s) 76, 5011
DseDI GACNNNNNNGTC 2 cut(s) 76, 5011
EaeI YGGCCR 2 cut(s) 3711, 5071
Eam1104I CTCTTC 6 cut(s) 609, 2999, 3063, 4190, 5087, 5169
EarI CTCTTC 6 cut(s) 609, 2999, 3063, 4190, 5087, 5169
Ecl136II GAGCTC 1 cut(s) 2809
Eco130I CCWWGG 6 cut(s) 1628, 3240, 3674, 4715, 5306, 5548
Eco24I GRGCYC 3 cut(s) 2811, 5190, 5526
Eco31I GGTCTC 1 cut(s) 1380
Eco32I GATATC 2 cut(s) 2583, 4405
Eco47I GGWCC 5 cut(s) 78, 1508, 2701, 5271, 5325
Eco53kI GAGCTC 1 cut(s) 2809
Eco57I CTGAAG 8 cut(s) 3393, 3647, 4302, 4392, 4904, 4992, 5103, 5193
Eco72I CACGTG 1 cut(s) 2603
Eco81I CCTNAGG 1 cut(s) 4794
Eco88I CYCGRG 1 cut(s) 4498
EcoICRI GAGCTC 1 cut(s) 2809
EcoNI CCTNNNNNAGG 2 cut(s) 2900, 4719
EcoO109I RGGNCCY 3 cut(s) 265, 5271, 5523
EcoRII CCWGG 5 cut(s) 1091, 2728, 2895, 4882, 5045
EcoRV GATATC 2 cut(s) 2583, 4405
EcoT14I CCWWGG 6 cut(s) 1628, 3240, 3674, 4715, 5306, 5548
EcoT22I ATGCAT 4 cut(s) 1261, 2536, 5294, 5505
EcoT38I GRGCYC 3 cut(s) 2811, 5190, 5526
EgeI GGCGCC 1 cut(s) 1573
EheI GGCGCC 1 cut(s) 1573
ErhI CCWWGG 6 cut(s) 1628, 3240, 3674, 4715, 5306, 5548
Esp3I CGTCTC 1 cut(s) 1745
FalI AAGNNNNNCTT 2 cut(s) 3233, 3265
FauI CCCGC 3 cut(s) 1151, 1992, 2302
FauNDI CATATG 3 cut(s) 2530, 3823, 5194
FbaI TGATCA 3 cut(s) 2479, 3640, 4624
FblI GTMKAC 4 cut(s) 300, 2989, 3942, 5343
FriOI GRGCYC 3 cut(s) 2811, 5190, 5526
GlaI GCGC 6 cut(s) 175, 480, 586, 588, 1552, 1573
GsaI CCCAGC 2 cut(s) 1495, 3428
GsuI CTGGAG 3 cut(s) 50, 2918, 5068
HaeII RGCGCY 1 cut(s) 1575
HaeIII GGCC 8 cut(s) 57, 266, 288, 1401, 1869, 3713, 5073, 5524
HapII CCGG 7 cut(s) 271, 707, 2181, 3394, 3747, 4499, 5419
HgaI GACGC 8 cut(s) 149, 236, 340, 878, 1834, 2921, 3701, 4577
HhaI GCGC 6 cut(s) 176, 481, 587, 589, 1553, 1574
Hin1I GRCGYC 5 cut(s) 141, 228, 1572, 2932, 2992
Hin6I GCGC 6 cut(s) 174, 479, 585, 587, 1551, 1572
HinP1I GCGC 6 cut(s) 174, 479, 585, 587, 1551, 1572
HincII GTYRAC 4 cut(s) 301, 1089, 2936, 3305
HindII GTYRAC 4 cut(s) 301, 1089, 2936, 3305
HindIII AAGCTT 3 cut(s) 2846, 3908, 3983
HpaII CCGG 7 cut(s) 271, 707, 2181, 3394, 3747, 4499, 5419
Hpy99I CGWCG 6 cut(s) 257, 356, 2454, 2997, 4485, 5364
HpyCH4IV ACGT 8 cut(s) 955, 2602, 2986, 2992, 3271, 3301, 4393, 5362
HpySE526I ACGT 8 cut(s) 955, 2602, 2986, 2992, 3271, 3301, 4393, 5362
Hsp92I GRCGYC 5 cut(s) 141, 228, 1572, 2932, 2992
HspAI GCGC 6 cut(s) 174, 479, 585, 587, 1551, 1572
KasI GGCGCC 1 cut(s) 1571
Kpn2I TCCGGA 1 cut(s) 270
KpnI GGTACC 1 cut(s) 5544
Ksp22I TGATCA 3 cut(s) 2479, 3640, 4624
LguI GCTCTTC 1 cut(s) 3063
LmnI GCTCC 8 cut(s) 155, 400, 514, 1333, 2899, 3823, 4576, 5119
MaeII ACGT 8 cut(s) 955, 2602, 2986, 2992, 3271, 3301, 4393, 5362
MfeI CAATTG 2 cut(s) 1785, 3172
MlsI TGGCCA 1 cut(s) 3713
MluI ACGCGT 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 3713
Mly113I GGCGCC 1 cut(s) 1572
MlyI GAGTC 8 cut(s) 78, 296, 1186, 3541, 4718, 4944, 4952, 5346
MmeI TCCRAC 6 cut(s) 123, 950, 3718, 3741, 4090, 5439
Mox20I TGGCCA 1 cut(s) 3713
Mph1103I ATGCAT 4 cut(s) 1261, 2536, 5294, 5505
MroI TCCGGA 1 cut(s) 270
MroXI GAANNNNTTC 5 cut(s) 278, 2037, 3313, 3831, 5412
MscI TGGCCA 1 cut(s) 3713
MslI CAYNNNNRTG 7 cut(s) 1886, 1993, 2061, 2943, 3891, 4913, 5487
Msp20I TGGCCA 1 cut(s) 3713
MspA1I CMGCKG 4 cut(s) 835, 1592, 2792, 3064
MspCI CTTAAG 1 cut(s) 2543
MspI CCGG 7 cut(s) 271, 707, 2181, 3394, 3747, 4499, 5419
MspR9I CCNGG 7 cut(s) 1093, 2730, 2897, 4499, 4500, 4884, 5047
MunI CAATTG 2 cut(s) 1785, 3172
Mva1269I GAATGC 1 cut(s) 4038
MvaI CCWGG 5 cut(s) 1093, 2730, 2897, 4884, 5047
MvnI CGCG 4 cut(s) 174, 351, 587, 5586
NarI GGCGCC 1 cut(s) 1572
NciI CCSGG 2 cut(s) 4499, 4500
NcoI CCATGG 2 cut(s) 3674, 5306
NdeI CATATG 3 cut(s) 2530, 3823, 5194
NmeAIII GCCGAG 2 cut(s) 256, 543
NmuCI GTSAC 7 cut(s) 535, 1016, 1855, 3130, 3558, 4580, 4710
NruI TCGCGA 1 cut(s) 5586
NsiI ATGCAT 4 cut(s) 1261, 2536, 5294, 5505
NspI RCATGY 2 cut(s) 572, 5296
NspV TTCGAA 1 cut(s) 2467
OliI CACNNNNGTG 1 cut(s) 1886
PaeI GCATGC 1 cut(s) 5296
PagI TCATGA 3 cut(s) 1942, 2743, 3613
PaqCI CACCTGC 1 cut(s) 2893
PauI GCGCGC 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 3063
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 351, 2608, 2992
PctI GAATGC 1 cut(s) 4038
PdmI GAANNNNTTC 5 cut(s) 278, 2037, 3313, 3831, 5412
PflFI GACNNNGTC 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 5045
PinAI ACCGGT 1 cut(s) 3746
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 8 cut(s) 78, 296, 1185, 3541, 4717, 4943, 4952, 5346
PluTI GGCGCC 1 cut(s) 1575
PmaCI CACGTG 1 cut(s) 2603
PmlI CACGTG 1 cut(s) 2603
PpsI GAGTC 8 cut(s) 78, 296, 1185, 3541, 4717, 4943, 4952, 5346
Ppu21I YACGTR 2 cut(s) 956, 2603
PpuMI RGGWCCY 1 cut(s) 5271
PshAI GACNNNNGTC 1 cut(s) 3174
PshBI ATTAAT 4 cut(s) 1061, 3803, 4185, 5022
PsiI TTATAA 1 cut(s) 2922
Psp124BI GAGCTC 1 cut(s) 2811
Psp5II RGGWCCY 1 cut(s) 5271
Psp6I CCWGG 5 cut(s) 1091, 2728, 2895, 4882, 5045
PspCI CACGTG 1 cut(s) 2603
PspFI CCCAGC 2 cut(s) 1491, 3424
PspGI CCWGG 5 cut(s) 1091, 2728, 2895, 4882, 5045
PspOMI GGGCCC 1 cut(s) 5522
PspPPI RGGWCCY 1 cut(s) 5271
PstI CTGCAG 2 cut(s) 1075, 4462
PstNI CAGNNNCTG 6 cut(s) 838, 1772, 2024, 3092, 3416, 5015
PsyI GACNNNGTC 1 cut(s) 305
PteI GCGCGC 1 cut(s) 585
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 3 cut(s) 1592, 2792, 3064
RruI TCGCGA 1 cut(s) 5586
RseI CAYNNNNRTG 7 cut(s) 1886, 1993, 2061, 2943, 3891, 4913, 5487
SacI GAGCTC 1 cut(s) 2811
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 3063
ScaI AGTACT 3 cut(s) 2242, 4681, 4997
SchI GAGTC 8 cut(s) 78, 296, 1186, 3541, 4718, 4944, 4952, 5346
ScrFI CCNGG 7 cut(s) 1093, 2730, 2897, 4499, 4500, 4884, 5047
SfcI CTRYAG 9 cut(s) 790, 1071, 1884, 3969, 4458, 4536, 4548, 5227, 5446
SfoI GGCGCC 1 cut(s) 1573
SfuI TTCGAA 1 cut(s) 2467
SinI GGWCC 5 cut(s) 78, 1508, 2701, 5271, 5325
SmaI CCCGGG 1 cut(s) 4500
SmiMI CAYNNNNRTG 7 cut(s) 1886, 1993, 2061, 2943, 3891, 4913, 5487
SmlI CTYRAG 3 cut(s) 1533, 2543, 4767
SmoI CTYRAG 3 cut(s) 1533, 2543, 4767
SphI GCATGC 1 cut(s) 5296
SsiI CCGC 9 cut(s) 172, 835, 1144, 1999, 2309, 2699, 2929, 4048, 4167
SspDI GGCGCC 1 cut(s) 1571
SspI AATATT 1 cut(s) 826
SstI GAGCTC 1 cut(s) 2811
StyD4I CCNGG 7 cut(s) 1091, 2728, 2895, 4497, 4498, 4882, 5045
StyI CCWWGG 6 cut(s) 1628, 3240, 3674, 4715, 5306, 5548
TaiI ACGT 8 cut(s) 958, 2605, 2989, 2995, 3274, 3304, 4396, 5365
TaqII GACCGA 1 cut(s) 1806
TatI WGTACW 8 cut(s) 555, 2026, 2240, 3932, 4616, 4679, 4995, 5612
TauI GCSGC 2 cut(s) 838, 2932
TseFI GTSAC 7 cut(s) 535, 1016, 1855, 3130, 3558, 4580, 4710
Tsp45I GTSAC 7 cut(s) 535, 1016, 1855, 3130, 3558, 4580, 4710
TspGWI ACGGA 4 cut(s) 142, 295, 1769, 2978
TspMI CCCGGG 1 cut(s) 4498
Tth111I GACNNNGTC 1 cut(s) 305
Vha464I CTTAAG 1 cut(s) 2543
VneI GTGCAC 2 cut(s) 1762, 2064
VpaK11BI GGWCC 5 cut(s) 78, 1508, 2701, 5271, 5325
VspI ATTAAT 4 cut(s) 1061, 3803, 4185, 5022
XagI CCTNNNNNAGG 2 cut(s) 2900, 4719
XbaI TCTAGA 3 cut(s) 1163, 2260, 3178
XceI RCATGY 2 cut(s) 572, 5296
XcmI CCANNNNNNNNNTGG 1 cut(s) 2492
XmaI CCCGGG 1 cut(s) 4498
XmiI GTMKAC 4 cut(s) 300, 2989, 3942, 5343
XmnI GAANNNNTTC 5 cut(s) 278, 2037, 3313, 3831, 5412
ZraI GACGTC 1 cut(s) 2993
ZrmI AGTACT 3 cut(s) 2242, 4681, 4997
Zsp2I ATGCAT 4 cut(s) 1261, 2536, 5294, 5505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.