MD09G1184300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004044
Physical Location & Seq
Reverse (-)
15944251 .. 15965891
21641 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1184300.v1.1.491

Sequence Viewer

Length: 5646 bp
ATGGACACGATTCTGAACTCCGCCATATCAGAAATCTGTTCGGAGCTCCGAAATGGCGTCTCTCTCCAAACCCTCTGGCCGAGGCTCGAGCCCTCTCTCTCCTCCTCCAACCTCGACCTCTCTCCTCGCCTCAAGCAATCCCTATGGGACGGCCTCCGCGCCGTCCCAACCCTCAAGCTCCTCGCCCGAAACGCACCGTTTGACCCTGCAGACCCTTCCATCCAGTCCGTCCAAGACGCCGAGAAGCTCAACCTGAAAGTCGTGGCTGACGAGGGTCTCATGAACAACTTCATGGGCCTCTACCATGCCCGCTCCGCCAATGCCGACTTGCGCCCCCCTCAACAGAAAGCTCTCGAGCGTGTCGCCATGGCTAGAACGAATGGAATTACACAAAGTCAGGTTGCTAAGGAACTTGGAATTGAAGGGAGAAACTTTCATTATGTAGTGAAGAACCTTGAATGCCAAGGGCTAATTGTGAAGCAACCTGCATTAGTAAGGACAAAAGAAGCTGGTGATGAAGGGGAGTCAAGAAACATCCCGATTGTGAGTACCAACATGTTATACTTGTATCGACATGGAAAACATTTAGGCTCTCAACAGATGATTGAGATCACCAAGGAAGAACAGGCTAAGGAGAATTTTGGAAATGGTAAAGGAAGTCCTGCACATGGAGGCGGTTTATCTGGAAAATGTGTTAAAGAGGATGTGCTTGTGAAGGACTATCTACCAGAAATGCAGGCAATCTGTGATAAGCTTGAAGAAGCCAATGGCAAGGTTCTTGTTGTTTCGGATATTAAGAAAGATCTTGGTTACACTGGAACGCCTGGAGGACATAAAGCTTGGAGAACAGTCTGTCGCAGGTTGAAAGCTGCTGGTATGGTTGAGGTGTTTGAAGCTAAAGTGAATGACAAGGTTGAGTGCTGTCTGCGTTTTCCAGAAAATTATACTCCCACAAGTTTTGCGCCAAAAACTCTTCGGCATGTGGATGATCATTGTGATGAAGACCAGCAAATGAAGTTAAGAAAAAAGAAGAAGTGTGAAATCACTGATCAGCTTGTAGAACTTCCAATTGAGCAACAAATTTACGATTTGATACATGCCACAAGCACAGAGGGCTTGACTAGGGTTGAGGTTGTTGAGAGGCTTGGAATTAGCAACAAAAAGAATTACAACCGGCTTGCTACTATGTGGTCTAGGTTTGGGTTGAGTGTTCAGCCAGAAATGCAAAAAAAAGCCATGGCGTACAGGTTGCGCACATCTGAGGAACACAATTCTGGATCAGCTAATGCTTTTCATAATAAATCAGAAAATGCCAATGAAAATAAAACTGCTGACCTATGTGTTGGCAGCCCAAATGCTCTTGACAGGTCAGGTCGAAATCAAACTCATTCAGCCTATGATTGTTCAACTTTGAAGGGTTATACTGCTAGTCCTGGAAATGTGGAACATAGAGACATTAATACCGAGCCTTCTAATGGATATCCTCGGTTTAGTGAGTCTAACCAGATCCTCCTTTCTCCTGAAAATCCCCAACTAAAATTTCTTGAGCCAAGAGATATGACTGATAGTTTACTGAGCACAGCAATGGAAATAAATGGCACCTCAGCAGAAACACTACCAGCTGTGTTAAAGCCACTTGGTTCTGGATCAGATCCGAGGTATCCATGTCTGTCTTTGAGTGAGGATAGCACTCGAAGAGAAAAAAGGATACTTCAGCGGCTACAGGATGAGAAGTTTATTGTAAGAGCTGAGCTGCATAGGTGGCTTGTGAGTCTGGAGAAGGACAAGTGTACGACAACAGACAGGAAAACTATAGACCGAATTTTAAAGAAACTTCAGGAACTAGGGCACTGTAAGTGCATACATATTAATGTACCTGTTGTCACAAATTGTGGTCGTAGCCGTACCACTCTAGTGATTCTGCACCCATCTATTCAGAGTTTAACTCCCGAACTAGTTTCGGAAATTCATGATGCATGGAGGTCTTTTGAGATTCAAAGCCGTGGCCAATGTTCATCTCGATGGAAGAGCAGTGGATCAGTTCCTGTACTGAAAGATGTTCAGAGGACTCAGAACCATGCCAGTACAGATATCCGAGCTATGAAATCAGAAGCCATGCGTTCTAATGGCTTTATTCTGGCAAAGATGATTCGTGCAAAGATGCTACATAGTTTCCTGTGGGACTACATATGTAGTTCAGGATCTGATGGTGCTTTTTCATCTGGGATAGATGTCATTGAGCTGAGAAATCCGCACAGCAGTAGCAAATTGTTTTCACTGGAGGCAGCAATCAAGGCTATTCCAGTTGAATTGTATTTACAAGTTGTGGGCGCCACTAAAAAAATTGATGATATGCTTGAGAAGTGTAAGAGGGGTTTGTGTCTCTCTGACCTTTCAGCTGAGGAGTATAAGTCTCTAATGGACACCCATGCAACTGGAAGGCTCTCATTGGTCATTGAAATTTTACGGCGATTGAAGTTGATTCGACTTGTTGGTGATGAACGTTCAAAGGATGAAATCCTAGTTCCCCATGCCACTTCTACGCACGCATTGGAGCTTAAGCCTTATATAGAAGAACCACTTTCAAAAGATTCAATATCTTTGAGTTTTGGATCTCCTGATCTTCGCCCACGAATCAGACATGATTTTGTCCTCTCAAATAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCCGACCCAAGGGCTGCTTTGCATGCATTTCCTGGATCTGCTGTAAGTGAGGTCTCCCTGTACAAGTCGTGGACCAAAATCCGAGTTATGACAGCAGCTCAGCGTGATGAGCTCTTAAAGCGTGTAGACAAGGATGATGCAAGTGAAAAGCTTTCATTTAAAGAGTGTGGGAAGATTGCAAAGGATCTCAATTTGACCTTGGAGCAGGTGCTTCGTGTGTATCATGATAAGCGCCAAAAGCGTCTTCAGGGATTGCAAAATAGAAGTGATGAAATTCAGTCAAAAAAAAGAAGACGTGTTTCACGAAAAAGGAAGAGATCTTCTGAACAAGAGTCAGTAAAGTCAATTGAAATTGATGAGGTAACTGCACAGCTGGAAGAGCAGGGGCATGCTGCATTATCTCATACTGTGAACCAATCCATGGAAGAAACTGATCTTTTGGTCACTTCAGATAAGAATGACACACATTTGCAACCATTGGTTGATCGTCTAGAAACTGAGCAGGAACCTGAAAAAGATTTTAAAAAGTTAAAGTCAGCACGTGCTTCAACACGTCAACGAAGGTTTTCATGGACAGATGAGGCTGACAGGCACTTGATTATCCAATATGTAAGACACCGTGCATCTCTTGGGGCAAAATTTCACCGAGTAGATTGGGCTTCACTTACTGACCTTCCAGCACCTCCTAGTACCTGCCAAAAACGAATGGCATTACTCAAAAGTAACAGAAGATTTAGAATAGCTTTAATGAGGCTCTGCAACATGATCAGTGAACGATATGCAAAATTCCTGGAAAAGACCCAGAACAGGTCTCCAAGAAATGATGATTGCAGGCTGCTTTTGCGAGTTTCAGCAGGGGAAGATCGTAACGGTGTTGTTCCTAATAACAGTAATCATAATCAAGTGACAGGTGTTCAGGAAGAACCATGGGATGATTTTGATGACAATAACATTAAGAAAGCCCTTGAGGAGGTACTTCACTACAAGAGGATATCTAAATTGGATGCCTCTAAACGAATTGGATCTACCTGCGAGGACTGGTCAGATCGTAATACAAATTCAGAAGAATATGATCCCCAGGAATCTGAATTTATTGCATCAGCTGCTCTTCATGAGGATGCTCAGAACCATAGTGGAAGAGGATTGAAAATTTCTTCACGGAGATCAAGCTGTCAACAACTTAATGAAAAGTTTTTTAAGCTTTTGCATGGCGTTAATGTTAGTACACAAGTCTCGAAATCACTGGCTGTTTCTAATGCTGTAGAGCTCTTTAAGCTTGTCTTCTTGAGTACCTCAACAGCACCAGAAGTACCGAATCTGCTCGCAGAAATTATACGGCGTTACTCAGAGTGTGATCTGTTTGCGGCTTTCAACTATCTTAGAGAGAGAAAAATCATGGTAGGGGGCAATGACAGTCAACATTTTTCTCTTTCACAGCAGTTCTTGCATAATATTTCTATGTCACCCTTTCCTACTAATTCTGGGAAGAGAGCTAATAAATTCGCGCATTGGCTCTGTGAAAGAGATAAAGATCTTATGGAAGGGGGGATTGATCTCCCTTCAGATTTACAGTGCGGGGACATATTCCATTTGTTTGCTCTAGTTTCTTCTGGTGAACTGTCAATTTCCCCGTGCTTGCCAGATGAAGGCATGGGAGAGGCTGAAGATTTGAGAACCTCGAAACGTAAAATTGATAGCAATGATTTTTTAGATGGTGATAAGACTAAAAAGCTAAAATCATTTGTGGTGGGCGAAGGTGAAATTATTTCTCGTCGAGAAAAAGGTTTCCCTGGTATCAAGGTATCTGTATATCGTGCAGCATTTTCTACAGCTCATGCTGTAGATTTGTTCAAAGACGATACTCCAGTTGGAAAATTTTTTGGTGGAAGTTATCAACTAGTCAGTACTTCTGGTCTGAGTGCTTTGTCTCCGCCTGACCATATGAAAGAAATCCTTGATTCATGCAGTACTGTCTCTGTATTAGATAGCGAGTCACCTTGGGAAGGTATGGTGCGGTATGCTGAACATTTGCTGCCATCAAGTTCTGCTCAAGATCAATCAAGTCCCATCCGTCCCGAGGTCTTTAGGTCTGTTTACAGTGCTATTCAAAAGGCCGGAGACCAAGGACTGAGCATAAGAGATGTTTCCCGAATAGAAAATATCCCAGGAGAAAGGATGACAGAGTTCATCATTGACGTGCTTCAAACATTTGAACGAGTCTTAAAGGTCAATGCTTACGACTCTGTCCGTTTCGTGGATTCATTGTATCGTGACAAGTATTTTATGACTTCAGTACCCGGTAGTTGTCAAAATTTTGAACCAACTTCATCGAGAAAGCCACTAGGAGGCGTTGACGGCGATTTGATCCTTCATCCAAAAAACTGTGATATTGGTGGTGCTCATTCAAAGGGGGATATAATTATGAATGCTGATGATGTTCATAAAGTGACGTTTCTTAATTTCCCTGAAAAGGTTTTTGAGCTCTCAGATGAAAAACGAACTAGCTGTGTCCCAAAAGGCTGTATGGAAGGCAAAGAAGTTTCGCCTAGAGGGGATGATGTGGATGAATCTTCTAGATCCTCTTCTGGTAAACTATGTGTGCCAATATTGCCATGGATAAATGGTGATGGGACCATTAACAAGATTATTTACAAGGGACTCCGTAGACGTGTTCTTGGAGTTGTGATGCAAAATCCAGGGATGATAGAGGATGAAATTATTCGCCGCATGGATGTGCTGAATCCCCAGAGCTGCAGAAAGTTATTGGAGTTGTTGATTTTGGATAAACACATTTATGTGAAGAAGATGCATCATACTATGCCCAACGGGATCCCATCCGTACTAAGAACCCTCTTTGGAAGCAGCTTCACTGAACCAAAGCTAGTATGCCATGAGCATTTCTTTGCAAATCCTATGAGCACATCCTTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1882

Amino Acids

211.63

Weight (kDa)

8.1

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 101 1.7e-34 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 114 - 191 5e-15 B-block binding subunit of TFIIIC
DUF7599 PF24538 239 - 323 8.6e-35 Domain of unknown function (DUF7599)
DUF7646 PF24657 344 - 425 4.2e-34 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 559 - 668 4.3e-42 GTF3C1, extended winged-helix domain
DUF7647 PF24658 750 - 929 2.4e-86 Domain of unknown function (DUF7647)
DUF7645 PF24655 931 - 988 2.7e-29 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2908
Acc16I TGCGCA 1 cut(s) 1253
Acc36I ACCTGC 5 cut(s) 493, 849, 2908, 3413, 3749
AccB1I GGYRCC 2 cut(s) 1598, 2330
AccB7I CCANNNNNTGG 1 cut(s) 3133
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 2 cut(s) 2838, 5377
AccII CGCG 2 cut(s) 159, 4217
AclI AACGTT 1 cut(s) 2503
AcoI YGGCCR 2 cut(s) 77, 2005
AcuI CTGAAG 7 cut(s) 1697, 1820, 2942, 3144, 4257, 4395, 4986
AcvI CACGTG 1 cut(s) 3254
AcyI GRCGYC 3 cut(s) 57, 237, 2331
AflII CTTAAG 1 cut(s) 2558
AflIII ACRYGT 4 cut(s) 555, 3007, 3263, 5380
AhlI ACTAGT 2 cut(s) 1954, 4609
AjiI CACGTC 4 cut(s) 3008, 3266, 4909, 5381
AjnI CCWGG 8 cut(s) 823, 1432, 2743, 3501, 3789, 4501, 4876, 5407
AjuI GAANNNNNNNTTGG 4 cut(s) 1524, 1556, 2940, 2972
AleI CACNNNNGTG 1 cut(s) 1913
Alw21I GWGCWC 7 cut(s) 48, 1580, 2826, 3981, 5113, 5196, 5633
AlwNI CAGNNNCTG 2 cut(s) 2045, 2204
Ama87I CYCGRG 3 cut(s) 86, 353, 4787
AoxI GGCC 5 cut(s) 77, 151, 295, 2005, 4824
AseI ATTAAT 2 cut(s) 1458, 1869
Asp700I GAANNNNTTC 3 cut(s) 287, 3277, 5430
AspLEI GCGC 7 cut(s) 161, 333, 964, 1254, 2333, 2946, 4219
AspS9I GGNCC 3 cut(s) 295, 2784, 5343
AsuC2I CCSGG 1 cut(s) 5010
AvaI CYCGRG 3 cut(s) 86, 353, 4787
AvaII GGWCC 2 cut(s) 2784, 5343
BaeGI GKGCMC 1 cut(s) 1851
BaeI ACNNNNGTAYC 4 cut(s) 4563, 4596, 4989, 5022
BalI TGGCCA 1 cut(s) 2007
BamHI GGATCC 1 cut(s) 5541
BanI GGYRCC 2 cut(s) 1598, 2330
BanII GRGCYC 5 cut(s) 48, 93, 2826, 3981, 5196
BbrPI CACGTG 1 cut(s) 3254
BbsI GAAGAC 4 cut(s) 1008, 2948, 3010, 3985
Bbv12I GWGCWC 7 cut(s) 48, 1580, 2826, 3981, 5113, 5196, 5633
BbvCI CCTCAGC 2 cut(s) 1603, 2400
BccI CCATC 9 cut(s) 227, 1936, 2016, 2201, 4418, 4756, 4787, 5333, 5554
BceAI ACGGC 7 cut(s) 146, 166, 1887, 1986, 2483, 4064, 5083
BcgI CGANNNNNNTGC 2 cut(s) 2906, 2940
BciT130I CCWGG 8 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5409
BciVI GTATCC 2 cut(s) 1671, 1701
BclI TGATCA 3 cut(s) 988, 1048, 3477
BcnI CCSGG 1 cut(s) 5010
BcuI ACTAGT 2 cut(s) 1954, 4609
BfmI CTRYAG 7 cut(s) 207, 1721, 1812, 3972, 4539, 4551, 5464
BfoI RGCGCY 2 cut(s) 2334, 2947
BfrI CTTAAG 1 cut(s) 2558
BfuAI ACCTGC 5 cut(s) 493, 849, 2908, 3413, 3749
BfuI GTATCC 2 cut(s) 1671, 1701
BglII AGATCT 3 cut(s) 802, 3029, 4243
BlpI GCTNAGC 2 cut(s) 1749, 2811
BmcAI AGTACT 2 cut(s) 4618, 4681
Bme1390I CCNGG 9 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5010, 5409
Bme18I GGWCC 2 cut(s) 2784, 5343
BmeT110I CYCGRG 3 cut(s) 86, 353, 4787
BmgBI CACGTC 4 cut(s) 3008, 3266, 4909, 5381
BmgT120I GGNCC 3 cut(s) 295, 2784, 5343
BmiI GGNNCC 5 cut(s) 1600, 2332, 3219, 5344, 5543
BmrFI CCNGG 9 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5010, 5409
BoxI GACNNNNGTC 1 cut(s) 273
BpiI GAAGAC 4 cut(s) 1008, 2948, 3010, 3985
BplI GAGNNNNNCTC 2 cut(s) 1930, 1962
BpmI CTGGAG 4 cut(s) 846, 1796, 2300, 4560
Bpu10I CCTNAGC 4 cut(s) 405, 630, 1603, 2400
Bpu1102I GCTNAGC 2 cut(s) 1749, 2811
BpuEI CTTGAG 7 cut(s) 116, 158, 1565, 2378, 3698, 4018, 4746
BpuMI CCSGG 1 cut(s) 5010
BsaAI YACGTR 1 cut(s) 3254
BsaBI GATNNNNATC 2 cut(s) 608, 4242
BsaHI GRCGYC 3 cut(s) 57, 237, 2331
BsaI GGTCTC 4 cut(s) 281, 2770, 3528, 4824
BsaXI ACNNNNNCTCC 2 cut(s) 2396, 2426
Bse118I RCCGGY 1 cut(s) 1173
Bse1I ACTGG 9 cut(s) 223, 820, 2082, 2283, 2303, 2440, 3755, 3960, 4577
Bse3DI GCAATG 3 cut(s) 1590, 4126, 4417
Bse8I GATNNNNATC 2 cut(s) 608, 4242
BseBI CCWGG 8 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5409
BseJI GATNNNNATC 2 cut(s) 608, 4242
BseMI GCAATG 3 cut(s) 1590, 4126, 4417
BseNI ACTGG 9 cut(s) 223, 820, 2082, 2283, 2303, 2440, 3755, 3960, 4577
BseRI GAGGAG 6 cut(s) 91, 94, 114, 170, 2417, 3695
BseSI GKGCMC 1 cut(s) 1851
BsgI GTGCAG 4 cut(s) 648, 1907, 3063, 4548
Bsh1236I CGCG 2 cut(s) 159, 4217
BshFI GGCC 5 cut(s) 79, 153, 297, 2007, 4826
BshNI GGYRCC 2 cut(s) 1598, 2330
BsiHKAI GWGCWC 7 cut(s) 48, 1580, 2826, 3981, 5113, 5196, 5633
BsiHKCI CYCGRG 3 cut(s) 86, 353, 4787
BsiSI CCGG 3 cut(s) 1174, 4827, 5010
BslFI GGGAC 9 cut(s) 149, 161, 2195, 4304, 4761, 4770, 5207, 5356, 5382
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 9 cut(s) 149, 161, 2195, 4304, 4761, 4770, 5207, 5356, 5382
BsmI GAATGC 2 cut(s) 464, 5143
BsnI GGCC 5 cut(s) 79, 153, 297, 2007, 4826
Bso31I GGTCTC 4 cut(s) 281, 2770, 3528, 4824
BsoBI CYCGRG 3 cut(s) 86, 353, 4787
Bsp1286I GDGCHC 9 cut(s) 48, 93, 1580, 1851, 2826, 3981, 5113, 5196, 5633
Bsp1407I TGTACA 1 cut(s) 2772
Bsp1720I GCTNAGC 2 cut(s) 1749, 2811
Bsp19I CCATGG 5 cut(s) 366, 1236, 3132, 3638, 5324
BspANI GGCC 5 cut(s) 79, 153, 297, 2007, 4826
BspFNI CGCG 2 cut(s) 159, 4217
BspHI TCATGA 4 cut(s) 279, 1969, 2935, 3823
BspLI GGNNCC 5 cut(s) 1600, 2332, 3219, 5344, 5543
BspMAI CTGCAG 2 cut(s) 211, 5468
BspMI ACCTGC 5 cut(s) 493, 849, 2908, 3413, 3749
BspQI GCTCTTC 3 cut(s) 2021, 3084, 3825
BspT107I GGYRCC 2 cut(s) 1598, 2330
BspTI CTTAAG 1 cut(s) 2558
BspTNI GGTCTC 4 cut(s) 281, 2770, 3528, 4824
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 3 cut(s) 1590, 4126, 4417
BsrFI RCCGGY 1 cut(s) 1173
BsrGI TGTACA 1 cut(s) 2772
BsrI ACTGG 9 cut(s) 223, 820, 2082, 2283, 2303, 2440, 3755, 3960, 4577
BssAI RCCGGY 1 cut(s) 1173
BssNI GRCGYC 3 cut(s) 57, 237, 2331
Bst2UI CCWGG 8 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5409
Bst6I CTCTTC 9 cut(s) 978, 1690, 2021, 3020, 3084, 3825, 3844, 4193, 5299
BstACI GRCGYC 3 cut(s) 57, 237, 2331
BstAFI CTTAAG 1 cut(s) 2558
BstAPI GCANNNNNTGC 2 cut(s) 3815, 4156
BstAUI TGTACA 1 cut(s) 2772
BstBAI YACGTR 1 cut(s) 3254
BstC8I GCNNGC 9 cut(s) 310, 738, 1179, 2547, 2736, 3102, 3545, 4035, 4349
BstDSI CCRYGG 6 cut(s) 366, 1236, 2002, 3132, 3638, 5324
BstENI CCTNNNNNAGG 2 cut(s) 3680, 4713
BstFNI CGCG 2 cut(s) 159, 4217
BstH2I RGCGCY 2 cut(s) 2334, 2947
BstHHI GCGC 7 cut(s) 161, 333, 964, 1254, 2333, 2946, 4219
BstNI CCWGG 8 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5409
BstNSI RCATGY 5 cut(s) 559, 983, 1100, 2738, 3104
BstPAI GACNNNNGTC 1 cut(s) 273
BstSCI CCNGG 9 cut(s) 823, 1432, 2743, 3501, 3789, 4501, 4876, 5008, 5407
BstSFI CTRYAG 7 cut(s) 207, 1721, 1812, 3972, 4539, 4551, 5464
BstSLI GKGCMC 1 cut(s) 1851
BstUI CGCG 2 cut(s) 159, 4217
BstV2I GAAGAC 4 cut(s) 1008, 2948, 3010, 3985
BstXI CCANNNNNNTGG 1 cut(s) 2435
BsuI GTATCC 2 cut(s) 1671, 1701
BsuRI GGCC 5 cut(s) 79, 153, 297, 2007, 4826
BtgI CCRYGG 6 cut(s) 366, 1236, 2002, 3132, 3638, 5324
BtrI CACGTC 4 cut(s) 3008, 3266, 4909, 5381
BtsI GCAGTG 1 cut(s) 2038
BveI ACCTGC 5 cut(s) 493, 849, 2908, 3413, 3749
Cac8I GCNNGC 9 cut(s) 310, 738, 1179, 2547, 2736, 3102, 3545, 4035, 4349
CaiI CAGNNNCTG 2 cut(s) 2045, 2204
CciI TCATGA 4 cut(s) 279, 1969, 2935, 3823
CfoI GCGC 7 cut(s) 161, 333, 964, 1254, 2333, 2946, 4219
Cfr10I RCCGGY 1 cut(s) 1173
Cfr13I GGNCC 3 cut(s) 295, 2784, 5343
CseI GACGC 3 cut(s) 46, 245, 2942
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
DinI GGCGCC 1 cut(s) 2332
DraI TTTAAA 3 cut(s) 1827, 2872, 3235
EaeI YGGCCR 2 cut(s) 77, 2005
Eam1104I CTCTTC 9 cut(s) 978, 1690, 2021, 3020, 3084, 3825, 3844, 4193, 5299
EarI CTCTTC 9 cut(s) 978, 1690, 2021, 3020, 3084, 3825, 3844, 4193, 5299
EciI GGCGGA 3 cut(s) 10, 304, 4632
Ecl136II GAGCTC 4 cut(s) 46, 2824, 3979, 5194
Eco24I GRGCYC 5 cut(s) 48, 93, 2826, 3981, 5196
Eco31I GGTCTC 4 cut(s) 281, 2770, 3528, 4824
Eco32I GATATC 3 cut(s) 1481, 2092, 3705
Eco47I GGWCC 2 cut(s) 2784, 5343
Eco53kI GAGCTC 4 cut(s) 46, 2824, 3979, 5194
Eco57I CTGAAG 7 cut(s) 1697, 1820, 2942, 3144, 4257, 4395, 4986
Eco72I CACGTG 1 cut(s) 3254
Eco88I CYCGRG 3 cut(s) 86, 353, 4787
EcoICRI GAGCTC 4 cut(s) 46, 2824, 3979, 5194
EcoNI CCTNNNNNAGG 2 cut(s) 3680, 4713
EcoRII CCWGG 8 cut(s) 823, 1432, 2743, 3501, 3789, 4501, 4876, 5407
EcoRV GATATC 3 cut(s) 1481, 2092, 3705
EcoT22I ATGCAT 3 cut(s) 1978, 2740, 5523
EcoT38I GRGCYC 5 cut(s) 48, 93, 2826, 3981, 5196
EgeI GGCGCC 1 cut(s) 2332
EheI GGCGCC 1 cut(s) 2332
Esp3I CGTCTC 1 cut(s) 64
FalI AAGNNNNNCTT 8 cut(s) 2565, 2597, 2713, 2745, 3977, 4009, 4650, 4682
FaqI GGGAC 9 cut(s) 149, 161, 2195, 4304, 4761, 4770, 5207, 5356, 5382
FauI CCCGC 2 cut(s) 317, 4280
FauNDI CATATG 2 cut(s) 2189, 4653
FbaI TGATCA 3 cut(s) 988, 1048, 3477
FblI GTMKAC 2 cut(s) 2838, 5377
FriOI GRGCYC 5 cut(s) 48, 93, 2826, 3981, 5196
FspI TGCGCA 1 cut(s) 1253
GlaI GCGC 7 cut(s) 160, 332, 963, 1253, 2332, 2945, 4218
GsuI CTGGAG 4 cut(s) 846, 1796, 2300, 4560
HaeII RGCGCY 2 cut(s) 2334, 2947
HaeIII GGCC 5 cut(s) 79, 153, 297, 2007, 4826
HapII CCGG 3 cut(s) 1174, 4827, 5010
HgaI GACGC 3 cut(s) 46, 245, 2942
HhaI GCGC 7 cut(s) 161, 333, 964, 1254, 2333, 2946, 4219
Hin1I GRCGYC 3 cut(s) 57, 237, 2331
Hin6I GCGC 7 cut(s) 159, 331, 962, 1252, 2331, 2944, 4217
HinP1I GCGC 7 cut(s) 159, 331, 962, 1252, 2331, 2944, 4217
HincII GTYRAC 4 cut(s) 3269, 3887, 4130, 5065
HindII GTYRAC 4 cut(s) 3269, 3887, 4130, 5065
HindIII AAGCTT 5 cut(s) 752, 837, 2861, 3911, 3986
HpaII CCGG 3 cut(s) 1174, 4827, 5010
Hpy99I CGWCG 1 cut(s) 4488
HpyCH4IV ACGT 8 cut(s) 2503, 3007, 3253, 3265, 4396, 4908, 5162, 5380
HpySE526I ACGT 8 cut(s) 2503, 3007, 3253, 3265, 4396, 4908, 5162, 5380
Hsp92I GRCGYC 3 cut(s) 57, 237, 2331
HspAI GCGC 7 cut(s) 159, 331, 962, 1252, 2331, 2944, 4217
KasI GGCGCC 1 cut(s) 2330
Ksp22I TGATCA 3 cut(s) 988, 1048, 3477
LguI GCTCTTC 3 cut(s) 2021, 3084, 3825
LmnI GCTCC 6 cut(s) 43, 51, 183, 317, 2554, 2914
MaeII ACGT 8 cut(s) 2503, 3007, 3253, 3265, 4396, 4908, 5162, 5380
MbiI CCGCTC 1 cut(s) 312
MfeI CAATTG 2 cut(s) 1068, 3057
MhlI GDGCHC 9 cut(s) 48, 93, 1580, 1851, 2826, 3981, 5113, 5196, 5633
MlsI TGGCCA 1 cut(s) 2007
MluNI TGGCCA 1 cut(s) 2007
Mly113I GGCGCC 1 cut(s) 2331
MlyI GAGTC 9 cut(s) 533, 1505, 1780, 2062, 3053, 4712, 4938, 4946, 5364
MmeI TCCRAC 2 cut(s) 132, 4561
Mox20I TGGCCA 1 cut(s) 2007
Mph1103I ATGCAT 3 cut(s) 1978, 2740, 5523
MroXI GAANNNNTTC 3 cut(s) 287, 3277, 5430
MscI TGGCCA 1 cut(s) 2007
Msp20I TGGCCA 1 cut(s) 2007
MspA1I CMGCKG 5 cut(s) 1622, 1717, 2399, 3085, 3815
MspCI CTTAAG 1 cut(s) 2558
MspI CCGG 3 cut(s) 1174, 4827, 5010
MspR9I CCNGG 9 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5010, 5409
MunI CAATTG 2 cut(s) 1068, 3057
Mva1269I GAATGC 2 cut(s) 464, 5143
MvaI CCWGG 8 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5409
MvnI CGCG 2 cut(s) 159, 4217
NarI GGCGCC 1 cut(s) 2331
NciI CCSGG 1 cut(s) 5010
NcoI CCATGG 5 cut(s) 366, 1236, 3132, 3638, 5324
NdeI CATATG 2 cut(s) 2189, 4653
NlaIV GGNNCC 5 cut(s) 1600, 2332, 3219, 5344, 5543
NmeAIII GCCGAG 2 cut(s) 105, 265
NmuCI GTSAC 7 cut(s) 1882, 3154, 3616, 4173, 4704, 4982, 5158
NsbI TGCGCA 1 cut(s) 1253
NsiI ATGCAT 3 cut(s) 1978, 2740, 5523
NspI RCATGY 5 cut(s) 559, 983, 1100, 2738, 3104
OliI CACNNNNGTG 1 cut(s) 1913
PaeI GCATGC 2 cut(s) 2738, 3104
PaeR7I CTCGAG 2 cut(s) 86, 353
PagI TCATGA 4 cut(s) 279, 1969, 2935, 3823
PaqCI CACCTGC 1 cut(s) 2908
PciI ACATGT 1 cut(s) 555
PciSI GCTCTTC 3 cut(s) 2021, 3084, 3825
PcsI WCGNNNNNNNCGW 2 cut(s) 267, 3013
PctI GAATGC 2 cut(s) 464, 5143
PdmI GAANNNNTTC 3 cut(s) 287, 3277, 5430
PflFI GACNNNGTC 1 cut(s) 4955
PflMI CCANNNNNTGG 1 cut(s) 3133
PfoI TCCNGGA 3 cut(s) 1432, 2743, 3501
PleI GAGTC 9 cut(s) 532, 1504, 1779, 2062, 3052, 4711, 4937, 4946, 5364
PluTI GGCGCC 1 cut(s) 2334
PmaCI CACGTG 1 cut(s) 3254
PmlI CACGTG 1 cut(s) 3254
PpsI GAGTC 9 cut(s) 532, 1504, 1779, 2062, 3052, 4711, 4937, 4946, 5364
Ppu21I YACGTR 1 cut(s) 3254
PscI ACATGT 1 cut(s) 555
PshAI GACNNNNGTC 1 cut(s) 273
PshBI ATTAAT 2 cut(s) 1458, 1869
Psp124BI GAGCTC 4 cut(s) 48, 2826, 3981, 5196
Psp1406I AACGTT 1 cut(s) 2503
Psp6I CCWGG 8 cut(s) 823, 1432, 2743, 3501, 3789, 4501, 4876, 5407
PspCI CACGTG 1 cut(s) 3254
PspGI CCWGG 8 cut(s) 823, 1432, 2743, 3501, 3789, 4501, 4876, 5407
PspN4I GGNNCC 5 cut(s) 1600, 2332, 3219, 5344, 5543
PspPI GGNCC 3 cut(s) 295, 2784, 5343
PspXI VCTCGAGB 1 cut(s) 86
PstI CTGCAG 2 cut(s) 211, 5468
PstNI CAGNNNCTG 2 cut(s) 2045, 2204
PsyI GACNNNGTC 1 cut(s) 4955
PvuII CAGCTG 4 cut(s) 1622, 2399, 3085, 3815
SacI GAGCTC 4 cut(s) 48, 2826, 3981, 5196
SapI GCTCTTC 3 cut(s) 2021, 3084, 3825
Sau96I GGNCC 3 cut(s) 295, 2784, 5343
ScaI AGTACT 2 cut(s) 4618, 4681
SchI GAGTC 9 cut(s) 533, 1505, 1780, 2062, 3053, 4712, 4938, 4946, 5364
ScrFI CCNGG 9 cut(s) 825, 1434, 2745, 3503, 3791, 4503, 4878, 5010, 5409
SduI GDGCHC 9 cut(s) 48, 93, 1580, 1851, 2826, 3981, 5113, 5196, 5633
SfcI CTRYAG 7 cut(s) 207, 1721, 1812, 3972, 4539, 4551, 5464
SfoI GGCGCC 1 cut(s) 2332
Sfr274I CTCGAG 2 cut(s) 86, 353
SinI GGWCC 2 cut(s) 2784, 5343
SlaI CTCGAG 2 cut(s) 86, 353
SpeI ACTAGT 2 cut(s) 1954, 4609
SphI GCATGC 2 cut(s) 2738, 3104
SspDI GGCGCC 1 cut(s) 2330
SspI AATATT 2 cut(s) 4165, 5319
SstI GAGCTC 4 cut(s) 48, 2826, 3981, 5196
StyD4I CCNGG 9 cut(s) 823, 1432, 2743, 3501, 3789, 4501, 4876, 5008, 5407
TaiI ACGT 8 cut(s) 2506, 3010, 3256, 3268, 4399, 4911, 5165, 5383
TaqII GACCGA 1 cut(s) 1833
TatI WGTACW 6 cut(s) 2047, 2084, 2772, 3935, 4616, 4679
TauI GCSGC 3 cut(s) 1720, 4079, 5439
TseFI GTSAC 7 cut(s) 1882, 3154, 3616, 4173, 4704, 4982, 5158
Tsp45I GTSAC 7 cut(s) 1882, 3154, 3616, 4173, 4704, 4982, 5158
TspGWI ACGGA 6 cut(s) 217, 3886, 4772, 4949, 5363, 5539
Tth111I GACNNNGTC 1 cut(s) 4955
Van91I CCANNNNNTGG 1 cut(s) 3133
Vha464I CTTAAG 1 cut(s) 2558
VpaK11BI GGWCC 2 cut(s) 2784, 5343
VspI ATTAAT 2 cut(s) 1458, 1869
XagI CCTNNNNNAGG 2 cut(s) 3680, 4713
XbaI TCTAGA 2 cut(s) 3202, 5285
XceI RCATGY 5 cut(s) 559, 983, 1100, 2738, 3104
XcmI CCANNNNNNNNNTGG 1 cut(s) 5322
XhoI CTCGAG 2 cut(s) 86, 353
XmiI GTMKAC 2 cut(s) 2838, 5377
XmnI GAANNNNTTC 3 cut(s) 287, 3277, 5430
ZrmI AGTACT 2 cut(s) 4618, 4681
Zsp2I ATGCAT 3 cut(s) 1978, 2740, 5523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.