Rh2AG443400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004044
Physical Location & Seq
Forward (+)
65569124 .. 65580546
11423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG443400.1

Sequence Viewer

Length: 5658 bp
ATGGACTCCATTCTAAACTCAGCGATCGAAGAAATCTGCTCGCACCTCCAAAACGGCCTCTCTCTCCAGACCCTCTGGTCCCGACTCTCCTCCTCCTCCAACCTCGACCTCTCTCCCACCCTCAAACAATCCCTATGGGACGCCCTCCGCTCCGTCCCCACTCTCAAATTCAGCGCCCAAAAAGACCGTTACGCCCCCACCGACCCTTCCATTCACTCCTTCCAAGACGCCGAGAACCTCAATTTGAAGCTCGTCGCCGATGAGGCCCTCCGGAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAATGCCAACCTGTCTGCCGTTCAACGCCACACTCTCGAACGCGTCGTCATGGCTAGGGGGAATGGGATTACACAAACTCAGCTTGCTAAGGAGCTTGGCATTGAGGGGAGAAACTTTCATTATGCTGTGAAGAACCTCGAATGTCAAGGGTTACTTGTACGGCAATCTGCGCTGTTAAGGACAAAAGAAGCTGGTGATGAGGGGGAGCCGAGGAACAATCCGAGTGTGACAACGACCAATATGTTGTACTTGTATCGACATGCAAAACATTTAAGCGCACAACAAAAGATTGAGATCACAAAGGAAGAGAGGGCTAAGGAGAGTTTTGTAAATGTAAAAGAAAGCCCTGCAAGTGGAGATGGTTTTCCTGGAAATGTGCTCGTAAAGGACTATCTACCGGCAATGAAAGCAGTCTGTAATAAACTTGAAGAAGCCAATGGCAAGGTTCTTGTCGTTTCGGATATTAAGAAGGAACTTGGCTACAGTAAAACATCAGGGCATAGAGCTTGGAGAAATATTTGTCAGAGGCTGAAAGCTGCTCATCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGGAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAACGAAAGTTGAGCCAAGAGAAGTTGTACATGTAGATGAGGATTTTGTTGAAGAAGAACAAGTGAAATTCGGAAAGAAATGTAAAATCAGTGACCAGCTTGTAGAGCTTCCTATTGAGCATCAAATTTATGAATTAATAGATGCTGCAGGATCAGAGGGGTTGACCAGGAATGAGGTTATGGAGAGGCTTGGAATTGACAACAAGAAAAATTATACCCGCTTTGTTAGTATGTGCTCTAGATTTGATATGAGTCTGCAACCAGAAATGCACAAAAAAGCCGTGGCATATCGATTTCGGACATCTGGGAAACACAAGTCTGAATCAACTAATGCATTTCTTCGAAAATCAAAAGATGCCAATGACAGTAAAATTTTTAGTCTAAATGTTGGGAGTGTAGATGCTCTGAGATCAGATCAATTTCAAACTGGCTCAGTGTCAGATTGTTGGTCTCTGAACAGTGATACTGCTGGCCCTGAAAATATGAATAACATAGAGACTAATACTGACCCTTCTGCTGGATCTCTTGGGCGTAATGAGTTTGACGATATGCCTGAAACTTCCCAACAGTTACTTCTTGGACCAAAAGATACTACATCTGATTCTCAAGTTAGTTTAGTGAGCGCAGGAGTGGAACCAAATGGCGCCTTATCAGAAATACCAGCTGCTTTGTCAAAGCCACTTGCTAAAGGCTCAGATCCAAGGTATCCATGTATGTCTTTGACCGTGGATAACACTCGAAGGGAAAAGAGGATACTTGAACGGCTAGAGGGTGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGACAAGTGCACAGCAACTGACAGGAAAACAATTGACCGAATTTTACAAAAACTTCAACAGTTAGGGCACTGCAAGTGTATAGATATTAGCGTCCCTGTCGTCACAAATTTGGGCCGTACCCGAACCACTGTAGTGGTTCTGCATCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCTTGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAGTGGATCAGTTCCTGTGCTAGAAGATGTTCAGAGAACTCAGACCCATGTTAGTGCACAGCGACAAACACTGAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTGCTGCATAGTTTCCTGTGGGATTACCTGTATGGTTCGTCCGGCTCTAATGATGCTTTATCATTTGGAAAAGATGTTATTGAGCCAAGAGATCCTCACGGTACTAGAAAATTGTTTTCTCTAGAAGCAACAATGAAGGCTATTCCAGTTGAGTTGTTTCTACAAGTTGCGGGATCCACCAAAAACTTTGAGGATATGATTGAGAAATGTAAGAGGGGTTTATGTCTCTCTGATCTCTCTCGTGAAGAATACAAGTCTCTGATGGATACCCATGCAACTGGAAGGCTCTCATTGGTGATCGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGATCACCACTCAGAAAATGGATTCCAAGTTCCCCCTCCCATGATTTCTGCATATGCATTGGAACTTAAGCCTTATGTAGAGGAACCTGTTTCAAAAGATGCGATATCTTTGAGTTTTGGATCACGTGATCTTCGTCCACGAATCAGACATGATTTTTCCCTATCAACCAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCGGACCCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAGCGTGATGAGCTCTTAAAGCGTGTTGTGAAGGATGATCCAAGTGAAAAGCTTTCATTCAAAGAGTGTGGAAAAATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACACGGATGAAGTTCAATCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATGATGAGGTAACTGGACAGCTGGAAGAGCAGATACATCCCACAGTATCAGATACTGTGGAACAATTAGAAGAACTAAATCTCTTGGTCACTTCTTATGAGCATGATTCACATTTGCAAGCATTGGACAATTGTCTAGAAACTGGACAGGAACCTGAACCAAATGAAGATAATGAGGGGTGCCATTCAATTATTACTAAGGGTTCCTTTTCAAAGTTGAAGCCACTACGTCCTTCTAAAAGAAACAAGTTGCACTCAACACGTCAACGAAGGTTTTCATGGACAGAGGAGGCTGATAGGCAGTTGATTATCCAATATGTAAGACATCGTGCAACTCTTGGGGCAAAGATTCACCGGATAAATTGGGCTTCAGTTACTGACCTCCCAGCACCTCCTATTGCCTGCATGAAAAGAATGGCATCTCTGAAAAGTAACAGAAAGTTCAGATTAGCTGTAATGAGGCTTTGTAACATTCTCAGTGAACGATATGCAAAGATCTTGGAAAAGACTCAGAACAGGTCACTGAACAAAGATGATTGCAATCTGCTTTTGCGAGATTCAATAGGTGAAGGTCATGACAGCAAACTTCCCAATATCAGTGATCATAATCCAGAGACAGGTCTTCAGGAAGAACCATGGGATGATTTTGATGACGTTTATGTTAAAAAGTCCCTTGAGGAGGTACTTCACCACAAGAGGTTGGCCAAATTTGATGCCTCCAAAAGAGTTGGATCTACCGGTGAGGACTGGTCGGATCTTAATACAAGTGAATGTGATCCCCAGGAATCTGAATTAATTGTATCAACTGCTCCATATGAAGATGTTCAGAATCATGGTGGCAGAGAAAAAATTTCGGCACGAAGATCTTGCTATCAACACCTTAATGAAAAATACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACACAAGTCTACAAATCATTAGCTGTTTCTAATGCTGTAGAGCTGTTTAAGCTTGTTTTCCTCAGTACCTCAACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCATTCTACGGCGGTACTCAGAGTGTGATCTATTCGCAGCTTTCAACTATCTTAGAGATAAAAAGTTCATGGTTGGAGGTAATGGCAGTCAAAAATTTTCTCTCTCACAACAGTTCTTGCATAATACTTCCGCATCACCATTTCCAATTAATTCTGGGAAGAGAGCTACTAAATTCGCCCATTGGCTCTATGAAAAAGATAAAGATCTTATGGAAGGGGGGATCGATCTCTCCACAGATTTACAGTGTGGGGACATATTCCATTTGTTTGCTCTAGTTTCTTCAGGTGAACTGTCAATTTCTCCATGCTTGCCAGATGAAGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCAGATATCAATGAACTTTTAGATGATGAAAAAACTAAAAAGCTGAAATCATTTGTGGCTGCAGAAGGTGAAATCATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCACAGTATCTGTAAGTCGTGCAGAGTTTTCTATAGCCAATTCTATAGATTTGTTCAAGGAAGACGCTCCTATTGGTGACAAACATTTTGGGGGAAATCATCAACTAGAATGTACATCTGATCAGAGTAGCTTATCACATTCTGACTGTATGAAAGAAATCTTCAATTCAGGCAGTAATGTCCCTGTATTAGAGATTGGTTGCGAGTCACCTTGGGAAGGTATGGTGGCTTATGCCGAACATTTGTTGCCATTACATCCTGCTCAAGATCAGTCAAGTCCCATCCAGCCTGAGGTCTTTAGGACTGTTTACATTGCTATTCAGAAAGCTGGTGATCAAGGTCTGAGCATAGAAGAAGTTTCCCGAATAATGAATATCCCAGGAGAAAAGATGACTGATCTTATCATTGATGTGCTTCGGACATTTGAACGAGTCTTAAAGGTCAATGCTTATGACTCTATTCGTGTTGTGGATTCTTTGTATCGTGGCAAGTACTTTATGACTTCAGTGTCTGGAAGTAATCAAAAACTCGAACCACCTTCCTGGAGAAAGCCACTAGGCGATGACGGCCATATACTCTTCCATTCAGAAAATCGTGATAATGGTGCTGCTCCTTCAGAGACGGAGATAAATGCTGATGTCCATAAATTGACAATTCTTAATTTTCCTGAAGAGGTTGATGAGCCCTCATATGTAAAACAAATTAGCAGTGTTCCTGAAAGCTATAGGGAAGGAAAAGGAGGAGATGCTGAGGATGAATCTTCTAGGTCCTCTAATGATAGACTATGCATGCCTATATTTCCATGGATAAATGGTGATGGGACCACTAACAAGATTGTCTACAAGGGACTCAGGCGACGTGTGCTTGGGGTAGTGATGCAAAATCCAGCTATATTAGAGGATGAAATTATTCGCCGGATGGATGTATTGAATCCCCAGAGCTGTAGAAAATTGTTGGAATTGATGGTTTTGGATAACCATCTTCATGTGAGGAAGATGCATCAAACTGCGTGCATTGGGCCCCCACCAATTTTAGGTACCCTCCTTGGAAGCAGCTTCAAACCGTCAAAGCTGGTTTGTCGCGAGCACTATTTTGCAAATCCCATGAGTACATCCTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1885

Amino Acids

212.18

Weight (kDa)

6.47

Isoelectric Point (pI)

51.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 98 2.6e-34 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 109 - 192 1.2e-12 B-block binding subunit of TFIIIC
DUF7599 PF24538 232 - 314 7.3e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 333 - 417 1.1e-37 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 549 - 658 3.1e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 744 - 923 8.5e-83 Domain of unknown function (DUF7647)
DUF7645 PF24655 926 - 981 8.8e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2919
AarI CACCTGC 1 cut(s) 2890
AasI GACNNNNNNGTC 2 cut(s) 76, 5041
AatII GACGTC 1 cut(s) 2992
Acc36I ACCTGC 1 cut(s) 2890
Acc65I GGTACC 1 cut(s) 5570
AccB1I GGYRCC 3 cut(s) 1568, 3219, 5570
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 4 cut(s) 300, 2986, 3972, 5373
AccII CGCG 2 cut(s) 351, 5616
AccIII TCCGGA 1 cut(s) 270
AciI CCGC 8 cut(s) 148, 1144, 1996, 2306, 2696, 2926, 4078, 4197
AcoI YGGCCR 2 cut(s) 3741, 5101
AcuI CTGAAG 7 cut(s) 3393, 3647, 4332, 4422, 5022, 5133, 5223
AcvI CACGTG 1 cut(s) 2600
AcyI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
AflII CTTAAG 1 cut(s) 2540
AflIII ACRYGT 4 cut(s) 349, 955, 3299, 5392
AgeI ACCGGT 1 cut(s) 3776
AjiI CACGTC 2 cut(s) 3302, 5393
AjnI CCWGG 7 cut(s) 676, 1091, 2725, 2892, 3819, 4912, 5075
AjuI GAANNNNNNNTTGG 2 cut(s) 1482, 1514
AleI CACNNNNGTG 1 cut(s) 1883
AloI GAACNNNNNNTCC 2 cut(s) 2487, 2519
Alw21I GWGCWC 7 cut(s) 690, 1163, 1763, 1985, 2065, 2808, 5622
Alw26I GTCTC 7 cut(s) 1380, 1415, 1742, 2366, 2397, 3647, 5147
Alw44I GTGCAC 2 cut(s) 1759, 2061
AlwNI CAGNNNCTG 7 cut(s) 838, 1769, 2021, 3095, 3416, 4544, 5045
Ama87I CYCGRG 1 cut(s) 4528
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 8 cut(s) 55, 264, 286, 1396, 1864, 3741, 5101, 5552
ApaI GGGCCC 1 cut(s) 5556
ApaLI GTGCAC 2 cut(s) 1759, 2061
ArsI GACNNNNNNTTYG 4 cut(s) 339, 371, 4604, 4636
AseI ATTAAT 3 cut(s) 1061, 3833, 4215
AsiGI ACCGGT 1 cut(s) 3776
Asp700I GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
Asp718I GGTACC 1 cut(s) 5570
AspLEI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
AsuC2I CCSGG 2 cut(s) 4529, 4530
AsuII TTCGAA 2 cut(s) 1267, 2464
AvaI CYCGRG 1 cut(s) 4528
AvaII GGWCC 5 cut(s) 78, 1505, 2698, 5301, 5355
AxyI CCTNAGG 1 cut(s) 4824
BaeGI GKGCMC 4 cut(s) 1763, 1821, 2065, 5556
BalI TGGCCA 1 cut(s) 3743
BamHI GGATCC 1 cut(s) 2309
BanI GGYRCC 3 cut(s) 1568, 3219, 5570
BanII GRGCYC 3 cut(s) 2808, 5220, 5556
BarI GAAGNNNNNNTAC 2 cut(s) 3057, 3089
BauI CACGAG 1 cut(s) 2376
BbrPI CACGTG 1 cut(s) 2600
BbsI GAAGAC 2 cut(s) 3653, 4602
Bbv12I GWGCWC 7 cut(s) 690, 1163, 1763, 1985, 2065, 2808, 5622
BbvCI CCTCAGC 1 cut(s) 5283
BceAI ACGGC 8 cut(s) 70, 311, 485, 1190, 1703, 1851, 4091, 5116
BcgI CGANNNNNNTGC 4 cut(s) 2888, 2922, 3888, 3922
BciT130I CCWGG 7 cut(s) 678, 1093, 2727, 2894, 3821, 4914, 5077
BciVI GTATCC 3 cut(s) 1641, 1671, 2395
BclI TGATCA 4 cut(s) 2476, 3640, 4654, 4867
BcnI CCSGG 2 cut(s) 4529, 4530
BcoDI GTCTC 7 cut(s) 1380, 1415, 1742, 2366, 2397, 3647, 5147
BfmI CTRYAG 9 cut(s) 790, 1071, 1881, 3999, 4488, 4566, 4578, 5257, 5476
BfoI RGCGCY 2 cut(s) 177, 1572
BfrI CTTAAG 1 cut(s) 2540
BfuAI ACCTGC 1 cut(s) 2890
BfuI GTATCC 3 cut(s) 1641, 1671, 2395
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 4 cut(s) 3005, 3534, 3902, 4270
BlpI GCTNAGC 2 cut(s) 1719, 2793
BmcAI AGTACT 1 cut(s) 5027
Bme1390I CCNGG 9 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
Bme18I GGWCC 5 cut(s) 78, 1505, 2698, 5301, 5355
BmeT110I CYCGRG 1 cut(s) 4528
BmgBI CACGTC 2 cut(s) 3302, 5393
BmrFI CCNGG 9 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
BoxI GACNNNNGTC 1 cut(s) 3171
BpiI GAAGAC 2 cut(s) 3653, 4602
BplI GAGNNNNNCTC 2 cut(s) 1900, 1932
BpmI CTGGAG 3 cut(s) 50, 2915, 5098
Bpu10I CCTNAGC 3 cut(s) 396, 624, 5283
Bpu1102I GCTNAGC 2 cut(s) 1719, 2793
Bpu14I TTCGAA 2 cut(s) 1267, 2464
BpuEI CTTGAG 3 cut(s) 1515, 3734, 4782
BpuMI CCSGG 2 cut(s) 4529, 4530
Bsa29I ATCGAT 2 cut(s) 1216, 4290
BsaAI YACGTR 1 cut(s) 2600
BsaBI GATNNNNATC 5 cut(s) 602, 3579, 3645, 4269, 5511
BsaHI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BsaI GGTCTC 1 cut(s) 1380
BsaWI WCCGGW 3 cut(s) 270, 3393, 3776
BsaXI ACNNNNNCTCC 2 cut(s) 3942, 3972
Bse118I RCCGGY 2 cut(s) 706, 3776
Bse1I ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
Bse21I CCTNAGG 1 cut(s) 4824
Bse3DI GCAATG 2 cut(s) 717, 4845
Bse8I GATNNNNATC 5 cut(s) 602, 3579, 3645, 4269, 5511
BseAI TCCGGA 1 cut(s) 270
BseBI CCWGG 7 cut(s) 678, 1093, 2727, 2894, 3821, 4914, 5077
BseCI ATCGAT 2 cut(s) 1216, 4290
BseJI GATNNNNATC 5 cut(s) 602, 3579, 3645, 4269, 5511
BseMI GCAATG 2 cut(s) 717, 4845
BseNI ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
BseRI GAGGAG 6 cut(s) 79, 82, 85, 3341, 3731, 5289
BseSI GKGCMC 4 cut(s) 1763, 1821, 2065, 5556
BseYI CCCAGC 1 cut(s) 3424
BsgI GTGCAG 1 cut(s) 4575
Bsh1236I CGCG 2 cut(s) 351, 5616
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
BshNI GGYRCC 3 cut(s) 1568, 3219, 5570
BshTI ACCGGT 1 cut(s) 3776
BshVI ATCGAT 2 cut(s) 1216, 4290
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 7 cut(s) 690, 1163, 1763, 1985, 2065, 2808, 5622
BsiHKCI CYCGRG 1 cut(s) 4528
BsiSI CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
BsmAI GTCTC 7 cut(s) 1380, 1415, 1742, 2366, 2397, 3647, 5147
BsmBI CGTCTC 2 cut(s) 1742, 5147
BsmI GAATGC 1 cut(s) 4068
BsnI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
Bso31I GGTCTC 1 cut(s) 1380
BsoBI CYCGRG 1 cut(s) 4528
Bsp119I TTCGAA 2 cut(s) 1267, 2464
Bsp120I GGGCCC 1 cut(s) 5552
Bsp13I TCCGGA 1 cut(s) 270
Bsp1407I TGTACA 2 cut(s) 952, 4646
Bsp1720I GCTNAGC 2 cut(s) 1719, 2793
Bsp19I CCATGG 2 cut(s) 3674, 5336
Bsp68I TCGCGA 1 cut(s) 5616
BspACI CCGC 8 cut(s) 148, 1144, 1996, 2306, 2696, 2926, 4078, 4197
BspANI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
BspDI ATCGAT 2 cut(s) 1216, 4290
BspEI TCCGGA 1 cut(s) 270
BspFNI CGCG 2 cut(s) 351, 5616
BspHI TCATGA 3 cut(s) 1939, 2740, 3613
BspMAI CTGCAG 2 cut(s) 1075, 4492
BspMI ACCTGC 1 cut(s) 2890
BspQI GCTCTTC 1 cut(s) 3060
BspT104I TTCGAA 2 cut(s) 1267, 2464
BspT107I GGYRCC 3 cut(s) 1568, 3219, 5570
BspTI CTTAAG 1 cut(s) 2540
BspTNI GGTCTC 1 cut(s) 1380
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 2 cut(s) 717, 4845
BsrFI RCCGGY 2 cut(s) 706, 3776
BsrGI TGTACA 2 cut(s) 952, 4646
BsrI ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
BssAI RCCGGY 2 cut(s) 706, 3776
BssNI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BssSI CACGAG 1 cut(s) 2376
BssT1I CCWWGG 5 cut(s) 1625, 3674, 4745, 5336, 5578
Bst2BI CACGAG 1 cut(s) 2376
Bst2UI CCWGG 7 cut(s) 678, 1093, 2727, 2894, 3821, 4914, 5077
Bst6I CTCTTC 6 cut(s) 609, 2996, 3060, 4220, 5117, 5199
BstACI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BstAFI CTTAAG 1 cut(s) 2540
BstAUI TGTACA 2 cut(s) 952, 4646
BstBAI YACGTR 1 cut(s) 2600
BstBI TTCGAA 2 cut(s) 1267, 2464
BstDSI CCRYGG 4 cut(s) 1206, 1650, 3674, 5336
BstENI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
BstFNI CGCG 2 cut(s) 351, 5616
BstH2I RGCGCY 2 cut(s) 177, 1572
BstHHI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
BstMAI GTCTC 7 cut(s) 1380, 1415, 1742, 2366, 2397, 3647, 5147
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 7 cut(s) 678, 1093, 2727, 2894, 3821, 4914, 5077
BstNSI RCATGY 3 cut(s) 572, 959, 5326
BstPAI GACNNNNGTC 1 cut(s) 3171
BstSCI CCNGG 9 cut(s) 676, 1091, 2725, 2892, 3819, 4527, 4528, 4912, 5075
BstSFI CTRYAG 9 cut(s) 790, 1071, 1881, 3999, 4488, 4566, 4578, 5257, 5476
BstSLI GKGCMC 4 cut(s) 1763, 1821, 2065, 5556
BstUI CGCG 2 cut(s) 351, 5616
BstV2I GAAGAC 2 cut(s) 3653, 4602
BstXI CCANNNNNNTGG 3 cut(s) 1885, 2414, 5076
Bsu15I ATCGAT 2 cut(s) 1216, 4290
Bsu36I CCTNAGG 1 cut(s) 4824
BsuI GTATCC 3 cut(s) 1641, 1671, 2395
BsuRI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
BsuTUI ATCGAT 2 cut(s) 1216, 4290
BtgI CCRYGG 4 cut(s) 1206, 1650, 3674, 5336
BtgZI GCGATG 1 cut(s) 5109
BtrI CACGTC 2 cut(s) 3302, 5393
BtsI GCAGTG 2 cut(s) 1819, 5248
BtuMI TCGCGA 1 cut(s) 5616
BveI ACCTGC 1 cut(s) 2890
CaiI CAGNNNCTG 7 cut(s) 838, 1769, 2021, 3095, 3416, 4544, 5045
CciI TCATGA 3 cut(s) 1939, 2740, 3613
CfoI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
Cfr10I RCCGGY 2 cut(s) 706, 3776
Cfr9I CCCGGG 1 cut(s) 4528
ClaI ATCGAT 2 cut(s) 1216, 4290
CseI GACGC 7 cut(s) 149, 236, 340, 878, 1831, 2918, 4607
CspAI ACCGGT 1 cut(s) 3776
CspCI CAANNNNNGTGG 2 cut(s) 2842, 2877
DinI GGCGCC 1 cut(s) 1570
DrdI GACNNNNNNGTC 2 cut(s) 76, 5041
DseDI GACNNNNNNGTC 2 cut(s) 76, 5041
EaeI YGGCCR 2 cut(s) 3741, 5101
Eam1104I CTCTTC 6 cut(s) 609, 2996, 3060, 4220, 5117, 5199
EarI CTCTTC 6 cut(s) 609, 2996, 3060, 4220, 5117, 5199
Ecl136II GAGCTC 1 cut(s) 2806
Eco130I CCWWGG 5 cut(s) 1625, 3674, 4745, 5336, 5578
Eco24I GRGCYC 3 cut(s) 2808, 5220, 5556
Eco31I GGTCTC 1 cut(s) 1380
Eco32I GATATC 2 cut(s) 2580, 4435
Eco47I GGWCC 5 cut(s) 78, 1505, 2698, 5301, 5355
Eco53kI GAGCTC 1 cut(s) 2806
Eco57I CTGAAG 7 cut(s) 3393, 3647, 4332, 4422, 5022, 5133, 5223
Eco72I CACGTG 1 cut(s) 2600
Eco81I CCTNAGG 1 cut(s) 4824
Eco88I CYCGRG 1 cut(s) 4528
EcoICRI GAGCTC 1 cut(s) 2806
EcoNI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
EcoO109I RGGNCCY 3 cut(s) 265, 5301, 5553
EcoRII CCWGG 7 cut(s) 676, 1091, 2725, 2892, 3819, 4912, 5075
EcoRV GATATC 2 cut(s) 2580, 4435
EcoT14I CCWWGG 5 cut(s) 1625, 3674, 4745, 5336, 5578
EcoT22I ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
EcoT38I GRGCYC 3 cut(s) 2808, 5220, 5556
EgeI GGCGCC 1 cut(s) 1570
EheI GGCGCC 1 cut(s) 1570
ErhI CCWWGG 5 cut(s) 1625, 3674, 4745, 5336, 5578
Esp3I CGTCTC 2 cut(s) 1742, 5147
FalI AAGNNNNNCTT 4 cut(s) 447, 479, 3230, 3262
FauI CCCGC 3 cut(s) 1151, 1989, 2299
FauNDI CATATG 3 cut(s) 2527, 3853, 5224
FbaI TGATCA 4 cut(s) 2476, 3640, 4654, 4867
FblI GTMKAC 4 cut(s) 300, 2986, 3972, 5373
FriOI GRGCYC 3 cut(s) 2808, 5220, 5556
GlaI GCGC 5 cut(s) 175, 480, 586, 1549, 1570
GsaI CCCAGC 1 cut(s) 3428
GsuI CTGGAG 3 cut(s) 50, 2915, 5098
HaeII RGCGCY 2 cut(s) 177, 1572
HaeIII GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
HapII CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
HgaI GACGC 7 cut(s) 149, 236, 340, 878, 1831, 2918, 4607
HhaI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
Hin1I GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
Hin6I GCGC 5 cut(s) 174, 479, 585, 1548, 1569
HinP1I GCGC 5 cut(s) 174, 479, 585, 1548, 1569
HincII GTYRAC 4 cut(s) 301, 1089, 2933, 3305
HindII GTYRAC 4 cut(s) 301, 1089, 2933, 3305
HindIII AAGCTT 3 cut(s) 2843, 3938, 4013
HpaII CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
Hpy99I CGWCG 6 cut(s) 257, 356, 2451, 2994, 4515, 5394
HpyCH4IV ACGT 8 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 4423, 5392
HpySE526I ACGT 8 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 4423, 5392
Hsp92I GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
HspAI GCGC 5 cut(s) 174, 479, 585, 1548, 1569
KasI GGCGCC 1 cut(s) 1568
Kpn2I TCCGGA 1 cut(s) 270
KpnI GGTACC 1 cut(s) 5574
Ksp22I TGATCA 4 cut(s) 2476, 3640, 4654, 4867
LguI GCTCTTC 1 cut(s) 3060
LmnI GCTCC 7 cut(s) 155, 400, 514, 2896, 3853, 4606, 5149
MaeII ACGT 8 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 4423, 5392
MbiI CCGCTC 1 cut(s) 150
MfeI CAATTG 2 cut(s) 1782, 3169
MlsI TGGCCA 1 cut(s) 3743
MluI ACGCGT 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 3743
Mly113I GGCGCC 1 cut(s) 1569
MlyI GAGTC 8 cut(s) 78, 296, 1186, 3541, 4748, 4974, 4982, 5376
MmeI TCCRAC 6 cut(s) 123, 950, 3748, 3771, 4120, 5469
Mox20I TGGCCA 1 cut(s) 3743
Mph1103I ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
MroI TCCGGA 1 cut(s) 270
MroXI GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
MscI TGGCCA 1 cut(s) 3743
MslI CAYNNNNRTG 8 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943, 5517
Msp20I TGGCCA 1 cut(s) 3743
MspA1I CMGCKG 3 cut(s) 1589, 2789, 3061
MspCI CTTAAG 1 cut(s) 2540
MspI CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
MspR9I CCNGG 9 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
MunI CAATTG 2 cut(s) 1782, 3169
Mva1269I GAATGC 1 cut(s) 4068
MvaI CCWGG 7 cut(s) 678, 1093, 2727, 2894, 3821, 4914, 5077
MvnI CGCG 2 cut(s) 351, 5616
NarI GGCGCC 1 cut(s) 1569
NciI CCSGG 2 cut(s) 4529, 4530
NcoI CCATGG 2 cut(s) 3674, 5336
NdeI CATATG 3 cut(s) 2527, 3853, 5224
NmeAIII GCCGAG 2 cut(s) 256, 543
NmuCI GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
NruI TCGCGA 1 cut(s) 5616
NsiI ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
NspI RCATGY 3 cut(s) 572, 959, 5326
NspV TTCGAA 2 cut(s) 1267, 2464
OliI CACNNNNGTG 1 cut(s) 1883
PaeI GCATGC 1 cut(s) 5326
PagI TCATGA 3 cut(s) 1939, 2740, 3613
PaqCI CACCTGC 1 cut(s) 2890
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 3060
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 351, 2605, 2989
PctI GAATGC 1 cut(s) 4068
PdmI GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
PflFI GACNNNGTC 1 cut(s) 305
PfoI TCCNGGA 2 cut(s) 676, 5075
PinAI ACCGGT 1 cut(s) 3776
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 8 cut(s) 78, 296, 1185, 3541, 4747, 4973, 4982, 5376
PluTI GGCGCC 1 cut(s) 1572
PmaCI CACGTG 1 cut(s) 2600
PmlI CACGTG 1 cut(s) 2600
PpsI GAGTC 8 cut(s) 78, 296, 1185, 3541, 4747, 4973, 4982, 5376
Ppu21I YACGTR 1 cut(s) 2600
PpuMI RGGWCCY 1 cut(s) 5301
PscI ACATGT 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 3171
PshBI ATTAAT 3 cut(s) 1061, 3833, 4215
PsiI TTATAA 1 cut(s) 2919
Psp124BI GAGCTC 1 cut(s) 2808
Psp5II RGGWCCY 1 cut(s) 5301
Psp6I CCWGG 7 cut(s) 676, 1091, 2725, 2892, 3819, 4912, 5075
PspCI CACGTG 1 cut(s) 2600
PspFI CCCAGC 1 cut(s) 3424
PspGI CCWGG 7 cut(s) 676, 1091, 2725, 2892, 3819, 4912, 5075
PspOMI GGGCCC 1 cut(s) 5552
PspPPI RGGWCCY 1 cut(s) 5301
PstI CTGCAG 2 cut(s) 1075, 4492
PstNI CAGNNNCTG 7 cut(s) 838, 1769, 2021, 3095, 3416, 4544, 5045
PsyI GACNNNGTC 1 cut(s) 305
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 3 cut(s) 1589, 2789, 3061
RruI TCGCGA 1 cut(s) 5616
RseI CAYNNNNRTG 8 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943, 5517
SacI GAGCTC 1 cut(s) 2808
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 3060
ScaI AGTACT 1 cut(s) 5027
SchI GAGTC 8 cut(s) 78, 296, 1186, 3541, 4748, 4974, 4982, 5376
ScrFI CCNGG 9 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
SfcI CTRYAG 9 cut(s) 790, 1071, 1881, 3999, 4488, 4566, 4578, 5257, 5476
SfoI GGCGCC 1 cut(s) 1570
SfuI TTCGAA 2 cut(s) 1267, 2464
SinI GGWCC 5 cut(s) 78, 1505, 2698, 5301, 5355
SmaI CCCGGG 1 cut(s) 4530
SmiMI CAYNNNNRTG 8 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943, 5517
SmlI CTYRAG 4 cut(s) 1530, 2540, 3713, 4797
SmoI CTYRAG 4 cut(s) 1530, 2540, 3713, 4797
SphI GCATGC 1 cut(s) 5326
SsiI CCGC 8 cut(s) 148, 1144, 1996, 2306, 2696, 2926, 4078, 4197
SspDI GGCGCC 1 cut(s) 1568
SspI AATATT 1 cut(s) 826
SstI GAGCTC 1 cut(s) 2808
StyD4I CCNGG 9 cut(s) 676, 1091, 2725, 2892, 3819, 4527, 4528, 4912, 5075
StyI CCWWGG 5 cut(s) 1625, 3674, 4745, 5336, 5578
TaiI ACGT 8 cut(s) 2602, 2986, 2992, 3271, 3304, 3696, 4426, 5395
TaqII GACCGA 1 cut(s) 1803
TatI WGTACW 6 cut(s) 555, 952, 3962, 4646, 5025, 5642
TauI GCSGC 1 cut(s) 2929
TseFI GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
Tsp45I GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
TspGWI ACGGA 5 cut(s) 142, 295, 1766, 2975, 5171
TspMI CCCGGG 1 cut(s) 4528
Tth111I GACNNNGTC 1 cut(s) 305
Vha464I CTTAAG 1 cut(s) 2540
VneI GTGCAC 2 cut(s) 1759, 2061
VpaK11BI GGWCC 5 cut(s) 78, 1505, 2698, 5301, 5355
VspI ATTAAT 3 cut(s) 1061, 3833, 4215
XagI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
XbaI TCTAGA 3 cut(s) 1163, 2257, 3175
XceI RCATGY 3 cut(s) 572, 959, 5326
XcmI CCANNNNNNNNNTGG 1 cut(s) 2489
XmaI CCCGGG 1 cut(s) 4528
XmiI GTMKAC 4 cut(s) 300, 2986, 3972, 5373
XmnI GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
ZraI GACGTC 1 cut(s) 2990
ZrmI AGTACT 1 cut(s) 5027
Zsp2I ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.