Rroxscaffold_2G00100830

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004044
Physical Location & Seq
Reverse (-)
22701463 .. 22712814
11352 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00100830.1

Sequence Viewer

Length: 5262 bp
ATGAGGCCCTCCGGAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAATGCCAACCTGTCTGCCGTTCAACGCCACACTCTCGAACGCGTCGTCATGGCTAGGTTTCGGCGGTCATTATTCGAATGATTATTGCAGGGGGAATGGGATTACACAAACTCAGCTTGCTAAGGAGCTTGGCATTGAGGGGAGAAACTTTCATTATGCTGTGAAGAACCTTGAGTGTCAAGGGTTACTTGTACGGCAATCTGCGCTGTTAAGGACAAAAGAAGCTGGTGATGAGGGGGAGCTGAGGAACAATCCGAGTGTGACAACGACCAATATGTTGTACTTGTATCGACATGCAAAGCATTTGAGCGCGCAACAAAAGATTGAGATCACAAAGGAAGAGAGGGCTAAGGAGAGTTTTGTAAATGTAAAAGAAAGCCCTGCAAGTGGAGATGGTTTTGCTGGAAATGTGCTCGTAAAGGACTATCTACCGGCAATGAAAGCAGTCTGTGATAAACTTGAACAAGCCAATGGCAAGGTTCTTGTCGTCTCGGATATTAAGAAGGAACTTGGCTACAGTAAAACATCGGGGCATAGAGCTTGGAGAAATATTTGTCAGAGGCTGAAGGCTGCTCATCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGGAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAACAAAAGTTGAGCCAAGAGAAAATGTACATGTAGATGAGGATTTTGTTGAAGAAGAACAAGTGAAATTCGGAAAGAAATGTAAAATCAGTGACCAGCTTGTTGAGCTTCCTATTGAGCATCAAATTTATGAATTAATAGATGTTGCAGGATCAGAGGGGTTGACCAGGAATGAGGTTATGGAGAGGCTTGGAATTGACAACAAGAAAAATTATACCCGCTTTGTTAGTATGTGCTCTAGATTTGATATGAGTCTGCAACCAGAAATGCACAAAAAAGCCGTGGCATATCGATTTCGGACATCTGGGAAACACAAGTCTGAATCAACTAATGCATTTCTTCGAAAATCAAAAGATGCCAATGACAGTAAAATTTCTAGTCTAAATGTTGGGAGTGTAGATGCTCCGAGATCAGATCAATTTCAAACTGGCTCAGTGTCAGATTGTTGGTCTCTGAACGGTGATACTGCTGGCCCTGAAAATATGAATAACATAGAGACTAATACTGACCCTTCTGCTGGATCTCTTGGGCGTAATGAGTTTGACGATATGCCTGAAACTTCCCAACTGTTACTTCTTGGACCAAAAGATACTACATCTGATTTTCAAGTTAGCTTAGTGAGCGCAGGAGTGGAACCAAATGGCGCCTTATCAGAAATACCAGCTGCTTTGTCAAAGCCACTTACTAAAGGCTCAGATCCAAGGTATCCATGTATGTCTTTGACCGTGGATAACACCCGAAGGGAAAAGAGGATACTTGAACGGCTAGAGGGTGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGACAAGTGCACAGCAACTGACAGGAAAACAATTGACCGAATTTTACAAAAACTTCAACAGCTAGGGCACTGCAAGTGTATAGATATTAGCGTCCCTGTCGTCACAAATTTGGGCCGTACCCGAACCACTGTAGTGGTTCTGCATCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCTTGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAATGGATCAGTTCCTGTACTAGAAGATGTTCAGAGAACTCAGACCCATGTTAGTGCACAGCGACAAACACTGAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTGCTGCATAGTTTCCTGTGGGATTACCTGTATGGTTCGTCCGGCTCTAATGATGCTTTATCATTTGGAAAAGATGTTATTGAGCCAAGAGATCCTCACAGTACTAGAAAATTGTTTTCGCTAGAAGCAACAATGAAGGCTATTCCAGTTGAGTTGTTTCTACAAGTTGCGGGATCCACCAAAAACTTTGAGGATATGATTGAGAAATGTAAGAGTGGTTTATGTCTCTCTGATCTCTCTCGTGAAGAATACAAGTCTCTGATGGATACCCATGCAACTGGAAGGCTTTCATTGGTGATTGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGATCACCACTCAGAAAATGGATTCCAAGTTCCCCCTCCCATGATTTCTGCATATGCATTGGAGCTTAAGCCTTATGTAGAGGAACCTGTTTCAAAAGATGCGATATCTTTGAGTTTCGGATCACGTGATCTTCGTCCACGAATCAGACATGATTTTTCCCTATCAAGCAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCGGACCCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAGCGTGATGAGCTCTTAAAGCGTGTTGTGAAGGATGATCCAAGCGAAAAGCTTTCATTCAAAGAGTGTGGAAAAATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACACAGATGAAGTTCAATCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATGATGAGGTAACTGGACAGCTGGAAGAGCAGACACATCCCACAGTATCTGATACTGTGGAACAATTAGAAGAACTAAATCTCTTGGTCACTTCTTATGAGCATGATTCACATTTGCAAGCATTGGGCAATTGTCTAGAAACTGGGCAGGAACCTGAACCAAATGAAGATAATGAGGGGTGCCATTCAATTATTACCAAGGGTTCCTTTTCAAAGTTGAAGCCACTACGTCCTTCTAAAAGAAACAAGTTGCACTCAACACGTCAACGAAGGTTTTCATGGACAGAGGAGGCTGATAGGCAGTTGATTATCCAATATGTAAGACATCGTGCAACTCTTGGGGCAAAGATTCACCGGATAAATTGGGCTTCAGTTACTGATCTCCCAGCACCTCCTATTGCCTGCATGAAAAGAATGGCATCTCTGAAAAGTAACAAAAAGTTCAGATTAGCTGTAATGAGGCTTTGTAATATTCTCAGTGAACGATATGCAAAGATCTTGGAAAAGACTCAGAACAGGTCACTGAACAAAGATGATTGCAATCTGCTTTTGCGAGATTCAATAGGTGAAGGTCATGACAGCAAACTTCCCAATATCAGTGATCATAATCCAGAGACAGGTCTTCAGGAAGAACCATGGGATGATTTTGAGGACGCTTATGTTACAAAGTCCCTTGAGGAGGTACTTCACCACAAGAGGTTGGCCAAATTTGATGCCTCCAAAAGAGTTGGATCTACCGGTGAGGACTGGTCGGATCTTAATACAAGTGAATGTGATCCCCAGGAATCTGAATTAATTGCATCAACTGTTCCATATGAAGATGTTCAGAATCATGGTGGCAGAGAAAAACTTTCGGCACGAAGATCTTGCTATCAACACCTTAATGAAAAATACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACACAAGTCTACAAATCATTAGCTGTCTCTAATGCTGTAGAGCTGTTTAAGCTTGTTTTCCTCAGTACCTCAACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCATTCTACGGCGGTACTCAGAGTGTGATCTATTTGCAGCTTTCAACTATCTTAGAGATAAAAAGTTCATGGTTGGAGGTGAACTGTCAATTTCTCCATGCTTGCCAGATGAAGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCAGATATCAATGAACTTTTAGATGATGAAAAAACTAAAAAGCTGAAATCATTTGTGGCTGCAGAAGGTGAAATCATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCACGGTATCTGTAAGTCGTGCAGAGTTTTCTATAGCCAATTCTATAGATTTGTTCAAGGAAGACACTCCTATTGGTGACAAACATTTTGGGGGAAATCATCAACTAGAATGTACATCTGATCAGAGCAGCTTATCACATTCTGACTGTATGAAAGAAATCTTCAATTCAGGCAGTACTATCCCTGTATTAGAGATTCGTTGCGAGTCACCTTGGGAAGGTATGGTGGCTTATGCCGAACATTTGTTGCCATTACATCCTGCTCAAGATCAATCAAGTCCCATCCAGCCTGAGGTCTTTAGGGCTGTATACATTGCTATTCAGAAAGCTGGCGATCAAGGTCTGAGCATAGAAGAAGTTTCCCGAATAATGAATATCCCAGGAGAAAAGATGACTGATCTTATCATTGATGTGCTTCAGACATTTGAACGAGTCTTAAAGGTCAATGCTTATGACTCTATTCGTGTTGTGGATTCTTTGTATCGTGGCAAGTACTTTATGACTTCAGTGTCTGGAATTAATCAAAAACTCGAACCACCTTCCTGGAGAAAGCCACTAGGCGATGACGGCCATATACTCATCCATTCAGAAAATCGTGATAATGGTGCTGCTACTTCAGAGAGGGAGATAAATGCTGATGTCCATAAATTGACAATTCTTAATTTTCCTGAAGAGGTTGAGGAGCCCTCATATGTTAAACAAATTAGCAGTGTTCCTGAAAGCTATAGGGAAGGAAAAGGAGGAGATGCTGAGGATGAATCTTCTGGGTCCTCTAATGATAGACTATGCATGCCTATATTTCCATGGATAAATGGTGATGGGACCACTAACAAGATTGTCTACAAGGGACTCAGGCGACGTGTGCTTGGGATTGTGATGCAAAATCCAGCTATACTAGAGGATGAAATTATTCGCCGGATGGATGTATTGAATCCCCAGAGCTGTAGAAAATTGTTGGAAATTATGGTTTTGGATAACCATCTTCATGTGAGGAAGATGCATCAAACTACGTGCACTGGGCCCCCACCAATTTTAGGTACCGTCCTTGGAAGCAGCTTCAAACCATCAAAGCTGTTTTGTCGCGAGCACTATTTTGCAAATCCCATGACTCTGCAATCTGCATATGCTGAGCTCTGCAATGTGTATAGTAGGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1753

Amino Acids

197.43

Weight (kDa)

6.53

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B-block_TFIIIC PF04182 47 - 117 3.4e-09 B-block binding subunit of TFIIIC
DUF7599 PF24538 157 - 239 3.1e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 258 - 342 6.7e-37 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 474 - 583 2.9e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 669 - 848 1.5e-83 Domain of unknown function (DUF7647)
DUF7645 PF24655 851 - 906 8.1e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2694
AarI CACCTGC 1 cut(s) 2665
AasI GACNNNNNNGTC 1 cut(s) 4609
AatII GACGTC 1 cut(s) 2767
Acc36I ACCTGC 1 cut(s) 2665
Acc65I GGTACC 1 cut(s) 5138
AccB1I GGYRCC 3 cut(s) 1343, 2994, 5138
AccI GTMKAC 5 cut(s) 41, 2761, 3747, 4410, 4941
AccII CGCG 3 cut(s) 92, 362, 5184
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 7 cut(s) 114, 919, 1771, 2081, 2471, 2701, 3853
AcoI YGGCCR 2 cut(s) 3516, 4669
AcsI RAATTY 8 cut(s) 691, 767, 825, 1071, 1566, 1633, 1710, 3521
AcuI CTGAAG 8 cut(s) 635, 3168, 3422, 3990, 4502, 4590, 4701, 4791
AcvI CACGTG 1 cut(s) 2375
AcyI GRCGYC 3 cut(s) 1344, 2704, 2764
AflII CTTAAG 1 cut(s) 2315
AflIII ACRYGT 4 cut(s) 90, 730, 3074, 4960
AgeI ACCGGT 1 cut(s) 3551
AjiI CACGTC 2 cut(s) 3077, 4961
AjnI CCWGG 6 cut(s) 866, 2500, 2667, 3594, 4480, 4643
AjuI GAANNNNNNNTTGG 2 cut(s) 1257, 1289
AleI CACNNNNGTG 1 cut(s) 1658
AloI GAACNNNNNNTCC 2 cut(s) 2262, 2294
Alw21I GWGCWC 9 cut(s) 465, 938, 1538, 1760, 1840, 2583, 5117, 5190, 5235
Alw26I GTCTC 8 cut(s) 544, 1155, 1190, 1517, 2141, 2172, 3422, 3769
Alw44I GTGCAC 3 cut(s) 1534, 1836, 5113
AlwNI CAGNNNCTG 6 cut(s) 613, 1544, 1796, 2864, 3191, 4613
Ama87I CYCGRG 1 cut(s) 4096
Aor13HI TCCGGA 1 cut(s) 11
AoxI GGCC 7 cut(s) 5, 27, 1171, 1639, 3516, 4669, 5120
ApaI GGGCCC 1 cut(s) 5124
ApaLI GTGCAC 3 cut(s) 1534, 1836, 5113
ApoI RAATTY 8 cut(s) 691, 767, 825, 1071, 1566, 1633, 1710, 3521
ArsI GACNNNNNNTTYG 4 cut(s) 80, 112, 4172, 4204
AseI ATTAAT 3 cut(s) 836, 3608, 4620
AsiGI ACCGGT 1 cut(s) 3551
Asp700I GAANNNNTTC 6 cut(s) 19, 1809, 2197, 3088, 3636, 5010
Asp718I GGTACC 1 cut(s) 5138
AspLEI GCGC 5 cut(s) 256, 362, 364, 1325, 1346
AsuC2I CCSGG 2 cut(s) 4097, 4098
AsuII TTCGAA 3 cut(s) 125, 1042, 2239
AvaI CYCGRG 1 cut(s) 4096
AvaII GGWCC 4 cut(s) 1280, 2473, 4869, 4923
AxyI CCTNAGG 1 cut(s) 4392
BaeGI GKGCMC 5 cut(s) 1538, 1596, 1840, 5117, 5124
BalI TGGCCA 1 cut(s) 3518
BamHI GGATCC 1 cut(s) 2084
BanI GGYRCC 3 cut(s) 1343, 2994, 5138
BanII GRGCYC 4 cut(s) 2583, 4788, 5124, 5235
BauI CACGAG 1 cut(s) 2151
BbrPI CACGTG 1 cut(s) 2375
BbsI GAAGAC 2 cut(s) 3428, 4170
Bbv12I GWGCWC 9 cut(s) 465, 938, 1538, 1760, 1840, 2583, 5117, 5190, 5235
BbvCI CCTCAGC 2 cut(s) 293, 4851
BceAI ACGGC 7 cut(s) 52, 260, 965, 1478, 1626, 3866, 4684
BcgI CGANNNNNNTGC 4 cut(s) 2663, 2697, 3663, 3697
BciT130I CCWGG 6 cut(s) 868, 2502, 2669, 3596, 4482, 4645
BciVI GTATCC 3 cut(s) 1416, 1446, 2170
BclI TGATCA 3 cut(s) 2251, 3415, 4222
BcnI CCSGG 2 cut(s) 4097, 4098
BcoDI GTCTC 8 cut(s) 544, 1155, 1190, 1517, 2141, 2172, 3422, 3769
BfmI CTRYAG 8 cut(s) 565, 1656, 3774, 4056, 4134, 4146, 4825, 5044
BfoI RGCGCY 1 cut(s) 1347
BfrI CTTAAG 1 cut(s) 2315
BfuAI ACCTGC 1 cut(s) 2665
BfuI GTATCC 3 cut(s) 1416, 1446, 2170
BglII AGATCT 3 cut(s) 2780, 3309, 3677
BlpI GCTNAGC 3 cut(s) 1494, 2568, 5229
BmcAI AGTACT 3 cut(s) 2014, 4279, 4595
Bme1390I CCNGG 8 cut(s) 868, 2502, 2669, 3596, 4097, 4098, 4482, 4645
Bme18I GGWCC 4 cut(s) 1280, 2473, 4869, 4923
BmeT110I CYCGRG 1 cut(s) 4096
BmgBI CACGTC 2 cut(s) 3077, 4961
BmrFI CCNGG 8 cut(s) 868, 2502, 2669, 3596, 4097, 4098, 4482, 4645
BmrI ACTGGG 2 cut(s) 2967, 5127
BmuI ACTGGG 2 cut(s) 2967, 5127
BpiI GAAGAC 2 cut(s) 3428, 4170
BplI GAGNNNNNCTC 4 cut(s) 1675, 1707, 4772, 4804
BpmI CTGGAG 2 cut(s) 2690, 4666
Bpu10I CCTNAGC 4 cut(s) 171, 293, 399, 4851
Bpu1102I GCTNAGC 3 cut(s) 1494, 2568, 5229
Bpu14I TTCGAA 3 cut(s) 125, 1042, 2239
BpuEI CTTGAG 3 cut(s) 242, 3509, 4350
BpuMI CCSGG 2 cut(s) 4097, 4098
Bsa29I ATCGAT 1 cut(s) 991
BsaAI YACGTR 2 cut(s) 2375, 5112
BsaBI GATNNNNATC 4 cut(s) 377, 3354, 3420, 5079
BsaHI GRCGYC 3 cut(s) 1344, 2704, 2764
BsaI GGTCTC 1 cut(s) 1155
BsaWI WCCGGW 3 cut(s) 11, 3168, 3551
BsaXI ACNNNNNCTCC 2 cut(s) 3717, 3747
Bse118I RCCGGY 2 cut(s) 481, 3551
Bse1I ACTGG 7 cut(s) 1132, 2057, 2194, 2833, 2962, 3566, 5122
Bse21I CCTNAGG 1 cut(s) 4392
Bse3DI GCAATG 3 cut(s) 492, 4413, 5245
Bse8I GATNNNNATC 4 cut(s) 377, 3354, 3420, 5079
BseAI TCCGGA 1 cut(s) 11
BseBI CCWGG 6 cut(s) 868, 2502, 2669, 3596, 4482, 4645
BseCI ATCGAT 1 cut(s) 991
BseJI GATNNNNATC 4 cut(s) 377, 3354, 3420, 5079
BseMI GCAATG 3 cut(s) 492, 4413, 5245
BseNI ACTGG 7 cut(s) 1132, 2057, 2194, 2833, 2962, 3566, 5122
BsePI GCGCGC 1 cut(s) 360
BseRI GAGGAG 4 cut(s) 3116, 3506, 4796, 4857
BseSI GKGCMC 5 cut(s) 1538, 1596, 1840, 5117, 5124
BseYI CCCAGC 1 cut(s) 3199
BsgI GTGCAG 1 cut(s) 4143
Bsh1236I CGCG 3 cut(s) 92, 362, 5184
BshFI GGCC 7 cut(s) 7, 29, 1173, 1641, 3518, 4671, 5122
BshNI GGYRCC 3 cut(s) 1343, 2994, 5138
BshTI ACCGGT 1 cut(s) 3551
BshVI ATCGAT 1 cut(s) 991
BsiHKAI GWGCWC 9 cut(s) 465, 938, 1538, 1760, 1840, 2583, 5117, 5190, 5235
BsiHKCI CYCGRG 1 cut(s) 4096
BsiSI CCGG 7 cut(s) 12, 482, 1953, 3169, 3552, 4097, 5017
BslFI GGGAC 6 cut(s) 1604, 2509, 3469, 4365, 4936, 4962
BsmAI GTCTC 8 cut(s) 544, 1155, 1190, 1517, 2141, 2172, 3422, 3769
BsmBI CGTCTC 2 cut(s) 544, 1517
BsmFI GGGAC 6 cut(s) 1604, 2509, 3469, 4365, 4936, 4962
BsmI GAATGC 1 cut(s) 3843
BsnI GGCC 7 cut(s) 7, 29, 1173, 1641, 3518, 4671, 5122
Bso31I GGTCTC 1 cut(s) 1155
BsoBI CYCGRG 1 cut(s) 4096
Bsp119I TTCGAA 3 cut(s) 125, 1042, 2239
Bsp120I GGGCCC 1 cut(s) 5120
Bsp13I TCCGGA 1 cut(s) 11
Bsp1407I TGTACA 2 cut(s) 727, 4214
Bsp1720I GCTNAGC 3 cut(s) 1494, 2568, 5229
Bsp19I CCATGG 2 cut(s) 3449, 4904
Bsp68I TCGCGA 1 cut(s) 5184
BspACI CCGC 7 cut(s) 114, 919, 1771, 2081, 2471, 2701, 3853
BspANI GGCC 7 cut(s) 7, 29, 1173, 1641, 3518, 4671, 5122
BspDI ATCGAT 1 cut(s) 991
BspEI TCCGGA 1 cut(s) 11
BspFNI CGCG 3 cut(s) 92, 362, 5184
BspHI TCATGA 3 cut(s) 1714, 2515, 3388
BspMAI CTGCAG 1 cut(s) 4060
BspMI ACCTGC 1 cut(s) 2665
BspQI GCTCTTC 1 cut(s) 2835
BspT104I TTCGAA 3 cut(s) 125, 1042, 2239
BspT107I GGYRCC 3 cut(s) 1343, 2994, 5138
BspTI CTTAAG 1 cut(s) 2315
BspTNI GGTCTC 1 cut(s) 1155
BsrDI GCAATG 3 cut(s) 492, 4413, 5245
BsrFI RCCGGY 2 cut(s) 481, 3551
BsrGI TGTACA 2 cut(s) 727, 4214
BsrI ACTGG 7 cut(s) 1132, 2057, 2194, 2833, 2962, 3566, 5122
BssAI RCCGGY 2 cut(s) 481, 3551
BssHII GCGCGC 1 cut(s) 360
BssNAI GTATAC 1 cut(s) 4411
BssNI GRCGYC 3 cut(s) 1344, 2704, 2764
BssSI CACGAG 1 cut(s) 2151
BssT1I CCWWGG 6 cut(s) 1400, 3012, 3449, 4313, 4904, 5146
Bst1107I GTATAC 1 cut(s) 4411
Bst2BI CACGAG 1 cut(s) 2151
Bst2UI CCWGG 6 cut(s) 868, 2502, 2669, 3596, 4482, 4645
Bst6I CTCTTC 4 cut(s) 384, 2771, 2835, 4767
BstACI GRCGYC 3 cut(s) 1344, 2704, 2764
BstAFI CTTAAG 1 cut(s) 2315
BstAUI TGTACA 2 cut(s) 727, 4214
BstBAI YACGTR 2 cut(s) 2375, 5112
BstBI TTCGAA 3 cut(s) 125, 1042, 2239
BstDSI CCRYGG 4 cut(s) 981, 1425, 3449, 4904
BstENI CCTNNNNNAGG 3 cut(s) 2672, 3491, 4317
BstFNI CGCG 3 cut(s) 92, 362, 5184
BstH2I RGCGCY 1 cut(s) 1347
BstHHI GCGC 5 cut(s) 256, 362, 364, 1325, 1346
BstMAI GTCTC 8 cut(s) 544, 1155, 1190, 1517, 2141, 2172, 3422, 3769
BstNI CCWGG 6 cut(s) 868, 2502, 2669, 3596, 4482, 4645
BstNSI RCATGY 3 cut(s) 347, 734, 4894
BstSCI CCNGG 8 cut(s) 866, 2500, 2667, 3594, 4095, 4096, 4480, 4643
BstSFI CTRYAG 8 cut(s) 565, 1656, 3774, 4056, 4134, 4146, 4825, 5044
BstSLI GKGCMC 5 cut(s) 1538, 1596, 1840, 5117, 5124
BstUI CGCG 3 cut(s) 92, 362, 5184
BstV2I GAAGAC 2 cut(s) 3428, 4170
BstXI CCANNNNNNTGG 3 cut(s) 1660, 2189, 4644
BstZ17I GTATAC 1 cut(s) 4411
Bsu15I ATCGAT 1 cut(s) 991
Bsu36I CCTNAGG 1 cut(s) 4392
BsuI GTATCC 3 cut(s) 1416, 1446, 2170
BsuRI GGCC 7 cut(s) 7, 29, 1173, 1641, 3518, 4671, 5122
BsuTUI ATCGAT 1 cut(s) 991
BtgI CCRYGG 4 cut(s) 981, 1425, 3449, 4904
BtgZI GCGATG 1 cut(s) 4677
BtrI CACGTC 2 cut(s) 3077, 4961
BtsI GCAGTG 2 cut(s) 1594, 4816
BtuMI TCGCGA 1 cut(s) 5184
BveI ACCTGC 1 cut(s) 2665
CaiI CAGNNNCTG 6 cut(s) 613, 1544, 1796, 2864, 3191, 4613
CciI TCATGA 3 cut(s) 1714, 2515, 3388
CfoI GCGC 5 cut(s) 256, 362, 364, 1325, 1346
Cfr10I RCCGGY 2 cut(s) 481, 3551
Cfr9I CCCGGG 1 cut(s) 4096
ClaI ATCGAT 1 cut(s) 991
CseI GACGC 5 cut(s) 81, 653, 1606, 2693, 3476
CspAI ACCGGT 1 cut(s) 3551
CspCI CAANNNNNGTGG 2 cut(s) 2617, 2652
DinI GGCGCC 1 cut(s) 1345
DrdI GACNNNNNNGTC 1 cut(s) 4609
DseDI GACNNNNNNGTC 1 cut(s) 4609
EaeI YGGCCR 2 cut(s) 3516, 4669
Eam1104I CTCTTC 4 cut(s) 384, 2771, 2835, 4767
EarI CTCTTC 4 cut(s) 384, 2771, 2835, 4767
Ecl136II GAGCTC 2 cut(s) 2581, 5233
Eco130I CCWWGG 6 cut(s) 1400, 3012, 3449, 4313, 4904, 5146
Eco24I GRGCYC 4 cut(s) 2583, 4788, 5124, 5235
Eco31I GGTCTC 1 cut(s) 1155
Eco32I GATATC 2 cut(s) 2355, 4003
Eco47I GGWCC 4 cut(s) 1280, 2473, 4869, 4923
Eco53kI GAGCTC 2 cut(s) 2581, 5233
Eco57I CTGAAG 8 cut(s) 635, 3168, 3422, 3990, 4502, 4590, 4701, 4791
Eco72I CACGTG 1 cut(s) 2375
Eco81I CCTNAGG 1 cut(s) 4392
Eco88I CYCGRG 1 cut(s) 4096
EcoICRI GAGCTC 2 cut(s) 2581, 5233
EcoNI CCTNNNNNAGG 3 cut(s) 2672, 3491, 4317
EcoO109I RGGNCCY 3 cut(s) 6, 4869, 5121
EcoRII CCWGG 6 cut(s) 866, 2500, 2667, 3594, 4480, 4643
EcoRV GATATC 2 cut(s) 2355, 4003
EcoT14I CCWWGG 6 cut(s) 1400, 3012, 3449, 4313, 4904, 5146
EcoT22I ATGCAT 4 cut(s) 1036, 2308, 4892, 5103
EcoT38I GRGCYC 4 cut(s) 2583, 4788, 5124, 5235
EgeI GGCGCC 1 cut(s) 1345
EheI GGCGCC 1 cut(s) 1345
ErhI CCWWGG 6 cut(s) 1400, 3012, 3449, 4313, 4904, 5146
Esp3I CGTCTC 2 cut(s) 544, 1517
FalI AAGNNNNNCTT 4 cut(s) 222, 254, 3005, 3037
FaqI GGGAC 6 cut(s) 1604, 2509, 3469, 4365, 4936, 4962
FauI CCCGC 3 cut(s) 926, 1764, 2074
FauNDI CATATG 4 cut(s) 2302, 3628, 4792, 5224
FbaI TGATCA 3 cut(s) 2251, 3415, 4222
FblI GTMKAC 5 cut(s) 41, 2761, 3747, 4410, 4941
FriOI GRGCYC 4 cut(s) 2583, 4788, 5124, 5235
GlaI GCGC 5 cut(s) 255, 361, 363, 1324, 1345
GsaI CCCAGC 1 cut(s) 3203
GsuI CTGGAG 2 cut(s) 2690, 4666
HaeII RGCGCY 1 cut(s) 1347
HaeIII GGCC 7 cut(s) 7, 29, 1173, 1641, 3518, 4671, 5122
HapII CCGG 7 cut(s) 12, 482, 1953, 3169, 3552, 4097, 5017
HgaI GACGC 5 cut(s) 81, 653, 1606, 2693, 3476
HhaI GCGC 5 cut(s) 256, 362, 364, 1325, 1346
Hin1I GRCGYC 3 cut(s) 1344, 2704, 2764
Hin6I GCGC 5 cut(s) 254, 360, 362, 1323, 1344
HinP1I GCGC 5 cut(s) 254, 360, 362, 1323, 1344
HincII GTYRAC 4 cut(s) 42, 864, 2708, 3080
HindII GTYRAC 4 cut(s) 42, 864, 2708, 3080
HindIII AAGCTT 3 cut(s) 2618, 3713, 3788
HpaII CCGG 7 cut(s) 12, 482, 1953, 3169, 3552, 4097, 5017
Hpy99I CGWCG 5 cut(s) 97, 2226, 2769, 4083, 4962
HpyCH4IV ACGT 8 cut(s) 2374, 2758, 2764, 3043, 3076, 3991, 4960, 5111
HpySE526I ACGT 8 cut(s) 2374, 2758, 2764, 3043, 3076, 3991, 4960, 5111
Hsp92I GRCGYC 3 cut(s) 1344, 2704, 2764
HspAI GCGC 5 cut(s) 254, 360, 362, 1323, 1344
KasI GGCGCC 1 cut(s) 1343
Kpn2I TCCGGA 1 cut(s) 11
KpnI GGTACC 1 cut(s) 5142
Ksp22I TGATCA 3 cut(s) 2251, 3415, 4222
LguI GCTCTTC 1 cut(s) 2835
LmnI GCTCC 6 cut(s) 175, 289, 1108, 2311, 2671, 4783
MaeII ACGT 8 cut(s) 2374, 2758, 2764, 3043, 3076, 3991, 4960, 5111
MfeI CAATTG 2 cut(s) 1557, 2944
MlsI TGGCCA 1 cut(s) 3518
MluI ACGCGT 1 cut(s) 90
MluNI TGGCCA 1 cut(s) 3518
Mly113I GGCGCC 1 cut(s) 1344
MlyI GAGTC 8 cut(s) 37, 961, 3316, 4316, 4542, 4550, 4944, 5203
MmeI TCCRAC 5 cut(s) 725, 3523, 3546, 3895, 5037
Mox20I TGGCCA 1 cut(s) 3518
Mph1103I ATGCAT 4 cut(s) 1036, 2308, 4892, 5103
MroI TCCGGA 1 cut(s) 11
MroXI GAANNNNTTC 6 cut(s) 19, 1809, 2197, 3088, 3636, 5010
MscI TGGCCA 1 cut(s) 3518
MslI CAYNNNNRTG 8 cut(s) 735, 1658, 1765, 1833, 2715, 3696, 4511, 5085
Msp20I TGGCCA 1 cut(s) 3518
MspA1I CMGCKG 3 cut(s) 1364, 2564, 2836
MspCI CTTAAG 1 cut(s) 2315
MspI CCGG 7 cut(s) 12, 482, 1953, 3169, 3552, 4097, 5017
MspR9I CCNGG 8 cut(s) 868, 2502, 2669, 3596, 4097, 4098, 4482, 4645
MunI CAATTG 2 cut(s) 1557, 2944
Mva1269I GAATGC 1 cut(s) 3843
MvaI CCWGG 6 cut(s) 868, 2502, 2669, 3596, 4482, 4645
MvnI CGCG 3 cut(s) 92, 362, 5184
NarI GGCGCC 1 cut(s) 1344
NciI CCSGG 2 cut(s) 4097, 4098
NcoI CCATGG 2 cut(s) 3449, 4904
NdeI CATATG 4 cut(s) 2302, 3628, 4792, 5224
NmuCI GTSAC 7 cut(s) 310, 791, 1627, 2902, 3333, 4178, 4308
NruI TCGCGA 1 cut(s) 5184
NsiI ATGCAT 4 cut(s) 1036, 2308, 4892, 5103
NspI RCATGY 3 cut(s) 347, 734, 4894
NspV TTCGAA 3 cut(s) 125, 1042, 2239
OliI CACNNNNGTG 1 cut(s) 1658
PaeI GCATGC 1 cut(s) 4894
PagI TCATGA 3 cut(s) 1714, 2515, 3388
PaqCI CACCTGC 1 cut(s) 2665
PauI GCGCGC 1 cut(s) 360
PciI ACATGT 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 2835
PcsI WCGNNNNNNNCGW 3 cut(s) 92, 2380, 2764
PctI GAATGC 1 cut(s) 3843
PdmI GAANNNNTTC 6 cut(s) 19, 1809, 2197, 3088, 3636, 5010
PflFI GACNNNGTC 1 cut(s) 46
PfoI TCCNGGA 1 cut(s) 4643
PinAI ACCGGT 1 cut(s) 3551
PleI GAGTC 8 cut(s) 37, 960, 3316, 4315, 4541, 4550, 4944, 5203
PluTI GGCGCC 1 cut(s) 1347
PmaCI CACGTG 1 cut(s) 2375
PmlI CACGTG 1 cut(s) 2375
PpsI GAGTC 8 cut(s) 37, 960, 3316, 4315, 4541, 4550, 4944, 5203
Ppu21I YACGTR 2 cut(s) 2375, 5112
PpuMI RGGWCCY 1 cut(s) 4869
PscI ACATGT 1 cut(s) 730
PshBI ATTAAT 3 cut(s) 836, 3608, 4620
PsiI TTATAA 1 cut(s) 2694
Psp124BI GAGCTC 2 cut(s) 2583, 5235
Psp5II RGGWCCY 1 cut(s) 4869
Psp6I CCWGG 6 cut(s) 866, 2500, 2667, 3594, 4480, 4643
PspCI CACGTG 1 cut(s) 2375
PspFI CCCAGC 1 cut(s) 3199
PspGI CCWGG 6 cut(s) 866, 2500, 2667, 3594, 4480, 4643
PspOMI GGGCCC 1 cut(s) 5120
PspPPI RGGWCCY 1 cut(s) 4869
PsrI GAACNNNNNNTAC 1 cut(s) 5237
PstI CTGCAG 1 cut(s) 4060
PstNI CAGNNNCTG 6 cut(s) 613, 1544, 1796, 2864, 3191, 4613
PsyI GACNNNGTC 1 cut(s) 46
PteI GCGCGC 1 cut(s) 360
PvuII CAGCTG 3 cut(s) 1364, 2564, 2836
RruI TCGCGA 1 cut(s) 5184
RseI CAYNNNNRTG 8 cut(s) 735, 1658, 1765, 1833, 2715, 3696, 4511, 5085
SacI GAGCTC 2 cut(s) 2583, 5235
SalI GTCGAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 2835
ScaI AGTACT 3 cut(s) 2014, 4279, 4595
SchI GAGTC 8 cut(s) 37, 961, 3316, 4316, 4542, 4550, 4944, 5203
ScrFI CCNGG 8 cut(s) 868, 2502, 2669, 3596, 4097, 4098, 4482, 4645
SfcI CTRYAG 8 cut(s) 565, 1656, 3774, 4056, 4134, 4146, 4825, 5044
SfoI GGCGCC 1 cut(s) 1345
SfuI TTCGAA 3 cut(s) 125, 1042, 2239
SinI GGWCC 4 cut(s) 1280, 2473, 4869, 4923
SmaI CCCGGG 1 cut(s) 4098
SmiMI CAYNNNNRTG 8 cut(s) 735, 1658, 1765, 1833, 2715, 3696, 4511, 5085
SmlI CTYRAG 4 cut(s) 221, 2315, 3488, 4365
SmoI CTYRAG 4 cut(s) 221, 2315, 3488, 4365
SphI GCATGC 1 cut(s) 4894
SsiI CCGC 7 cut(s) 114, 919, 1771, 2081, 2471, 2701, 3853
SspDI GGCGCC 1 cut(s) 1343
SspI AATATT 2 cut(s) 601, 3286
SstI GAGCTC 2 cut(s) 2583, 5235
StyD4I CCNGG 8 cut(s) 866, 2500, 2667, 3594, 4095, 4096, 4480, 4643
StyI CCWWGG 6 cut(s) 1400, 3012, 3449, 4313, 4904, 5146
TaiI ACGT 8 cut(s) 2377, 2761, 2767, 3046, 3079, 3994, 4963, 5114
TaqI TCGA 9 cut(s) 41, 86, 125, 340, 991, 1042, 2239, 4081, 4632
TaqII GACCGA 1 cut(s) 1578
TatI WGTACW 8 cut(s) 330, 727, 1798, 2012, 3737, 4214, 4277, 4593
TauI GCSGC 1 cut(s) 2704
TseFI GTSAC 7 cut(s) 310, 791, 1627, 2902, 3333, 4178, 4308
Tsp45I GTSAC 7 cut(s) 310, 791, 1627, 2902, 3333, 4178, 4308
TspGWI ACGGA 2 cut(s) 36, 1541
TspMI CCCGGG 1 cut(s) 4096
Tth111I GACNNNGTC 1 cut(s) 46
Vha464I CTTAAG 1 cut(s) 2315
VneI GTGCAC 3 cut(s) 1534, 1836, 5113
VpaK11BI GGWCC 4 cut(s) 1280, 2473, 4869, 4923
VspI ATTAAT 3 cut(s) 836, 3608, 4620
XagI CCTNNNNNAGG 3 cut(s) 2672, 3491, 4317
XapI RAATTY 8 cut(s) 691, 767, 825, 1071, 1566, 1633, 1710, 3521
XbaI TCTAGA 2 cut(s) 938, 2950
XceI RCATGY 3 cut(s) 347, 734, 4894
XcmI CCANNNNNNNNNTGG 1 cut(s) 2264
XmaI CCCGGG 1 cut(s) 4096
XmiI GTMKAC 5 cut(s) 41, 2761, 3747, 4410, 4941
XmnI GAANNNNTTC 6 cut(s) 19, 1809, 2197, 3088, 3636, 5010
ZraI GACGTC 1 cut(s) 2765
ZrmI AGTACT 3 cut(s) 2014, 4279, 4595
Zsp2I ATGCAT 4 cut(s) 1036, 2308, 4892, 5103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.