Rorug02G0390700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004044
Physical Location & Seq
Forward (+)
49931075 .. 49932040
966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0390700.1

Sequence Viewer

Length: 966 bp
ATGGCCATGAATGTGTTGTTCTGGAATTGTCGAGGGATTTGTAATCCTTCCACTGGGCGTGCTTTGAAGATGTTAATCTCACAACATCAGCCGAAATTGGTATTTCTTATTGAAACGAAAATCAGAGAAAGAGAGGAGTTCGACAGATTACAGCGTAACCTAGGGTTTGATCATGCAGAAGGCGTGATGAGCGTTGGGCTGGCTGGGGGACTGGGTTTGTTCTGGAATGACGATGTTACGCTCAAAATACGGCAGCCTTCTACGGCCCGTTTTATCGATGCAGTGGTTGATGAGGGAGACGGCCTCTTGCGGTGGCGTTTCACAAGTTTTTACGGCAATCCAGGTGCAGCAGATCGTCATGAATCCTGGGATCTTCTCCGGGGTTTGAGTGACGACGACAACTTACCGTGGGTTGTGCTGGGAGATTTTAATGAGATCTTGTTGGCCTCGGAGAAGCTGGATGGCAGACCACGATCAGAAAGTCAAATGCGAGGTTTCCGAGAGGCGCTGGGATATGCGGAATTGGTGGACCTTGGGTATTACGGATGCCCGTTTACTTGGAGTGATTTGGAGACGAAGGTTAGGTTGGATAGGGTGGTTGCTACGGTGGAGTGGTCTGATAGTTTCGGCCATTCTCGGGTGATCCATCTGCCGCCAAGCAAGTCTGATCTTATTCCGGTTTTGCTTTGTATCAGTGCAGTTCCGGTTGTTTCACAGAGGAGATATCATAGGTTTAAATTTGAATCGATGTGGTTGAAGCATGAGGAATGCCATGAGATCGTTCAGCAGCAGTGGCAACGCCCTGTTCTAGGGCAACCAATGTTTCTGGTGTCCAAGAAGATTGCTAGAACCAGTATTGCGTTAGATGTTTGGCAGCGCAATGTATTTGGTGATCGTCAAAAAGCAATATCGGTAACTCGTTCACGGTTGGAGTCGCTGACTTATTGGCAGAGAGGGGTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

321

Amino Acids

36.95

Weight (kDa)

7.7

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 7 - 204 7e-14 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 310, 518, 653
AclWI GGATC 2 cut(s) 378, 637
AcoI YGGCCR 2 cut(s) 3, 628
AcsI RAATTY 1 cut(s) 737
AfaI GTAC 1 cut(s) 960
AfiI CCNNNNNNNGG 3 cut(s) 53, 637, 809
AgsI TTSAA 4 cut(s) 67, 113, 743, 757
AjnI CCWGG 2 cut(s) 340, 365
AjuI GAANNNNNNNTTGG 2 cut(s) 569, 601
AluBI AGCT 1 cut(s) 457
AluI AGCT 1 cut(s) 457
Alw26I GTCTC 2 cut(s) 291, 566
AlwI GGATC 2 cut(s) 378, 637
Ama87I CYCGRG 1 cut(s) 636
AoxI GGCC 5 cut(s) 3, 264, 301, 444, 628
ApeKI GCWGC 4 cut(s) 253, 347, 787, 874
ApoI RAATTY 1 cut(s) 737
AspA2I CCTAGG 1 cut(s) 160
AspLEI GCGC 2 cut(s) 508, 879
AspS9I GGNCC 2 cut(s) 265, 529
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 2 cut(s) 652, 902
AvaI CYCGRG 1 cut(s) 636
AvaII GGWCC 1 cut(s) 529
AvrII CCTAGG 1 cut(s) 160
BalI TGGCCA 1 cut(s) 5
BbvI GCAGC 4 cut(s) 265, 359, 799, 886
BccI CCATC 2 cut(s) 455, 654
BceAI ACGGC 4 cut(s) 266, 279, 316, 349
BciT130I CCWGG 2 cut(s) 342, 367
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 291, 566
BfaI CTAG 3 cut(s) 161, 809, 846
BfoI RGCGCY 1 cut(s) 509
BglII AGATCT 1 cut(s) 435
BisI GCNGC 5 cut(s) 254, 348, 653, 788, 875
BlnI CCTAGG 1 cut(s) 160
BlsI GCNGC 5 cut(s) 255, 349, 654, 789, 876
Bme1390I CCNGG 3 cut(s) 342, 367, 380
Bme18I GGWCC 1 cut(s) 529
BmeT110I CYCGRG 1 cut(s) 636
BmgT120I GGNCC 2 cut(s) 265, 529
BmrFI CCNGG 3 cut(s) 342, 367, 380
BmrI ACTGGG 2 cut(s) 63, 221
BmsI GCATC 2 cut(s) 268, 536
BmuI ACTGGG 2 cut(s) 63, 221
BplI GAGNNNNNCTC 2 cut(s) 288, 320
BpuMI CCSGG 1 cut(s) 380
Bsa29I ATCGAT 2 cut(s) 276, 746
BsaBI GATNNNNATC 1 cut(s) 74
BsaJI CCNNGG 6 cut(s) 160, 366, 379, 407, 447, 532
BsaWI WCCGGW 2 cut(s) 676, 703
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 637, 809
Bse1I ACTGG 3 cut(s) 58, 216, 852
Bse3DI GCAATG 1 cut(s) 886
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 2 cut(s) 342, 367
BseCI ATCGAT 2 cut(s) 276, 746
BseDI CCNNGG 6 cut(s) 160, 366, 379, 407, 447, 532
BseGI GGATG 2 cut(s) 466, 551
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 3 cut(s) 53, 637, 809
BseMI GCAATG 1 cut(s) 886
BseNI ACTGG 3 cut(s) 58, 216, 852
BseRI GAGGAG 2 cut(s) 149, 733
BseXI GCAGC 4 cut(s) 265, 359, 799, 886
BseYI CCCAGC 3 cut(s) 203, 418, 508
BsgI GTGCAG 2 cut(s) 366, 717
BshFI GGCC 5 cut(s) 5, 266, 303, 446, 630
BshVI ATCGAT 2 cut(s) 276, 746
BsiHKCI CYCGRG 1 cut(s) 636
BsiSI CCGG 3 cut(s) 379, 677, 704
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 53, 637, 809
BsmAI GTCTC 2 cut(s) 291, 566
BsmBI CGTCTC 2 cut(s) 291, 566
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 773
BsnI GGCC 5 cut(s) 5, 266, 303, 446, 630
BsoBI CYCGRG 1 cut(s) 636
Bsp143I GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
BspACI CCGC 3 cut(s) 310, 518, 653
BspANI GGCC 5 cut(s) 5, 266, 303, 446, 630
BspDI ATCGAT 2 cut(s) 276, 746
BspHI TCATGA 1 cut(s) 358
BspPI GGATC 2 cut(s) 378, 637
BsrDI GCAATG 1 cut(s) 886
BsrI ACTGG 3 cut(s) 58, 216, 852
BssECI CCNNGG 6 cut(s) 160, 366, 379, 407, 447, 532
BssMI GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
BssT1I CCWWGG 2 cut(s) 160, 532
Bst2UI CCWGG 2 cut(s) 342, 367
Bst4CI ACNGT 3 cut(s) 408, 607, 927
BstC8I GCNNGC 2 cut(s) 60, 201
BstDSI CCRYGG 1 cut(s) 407
BstENI CCTNNNNNAGG 1 cut(s) 807
BstF5I GGATG 2 cut(s) 466, 551
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 508, 879
BstKTI GATC 9 cut(s) 172, 355, 373, 438, 476, 645, 670, 780, 895
BstMAI GTCTC 2 cut(s) 291, 566
BstMBI GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
BstMWI GCNNNNNNNGC 2 cut(s) 189, 793
BstNI CCWGG 2 cut(s) 342, 367
BstSCI CCNGG 3 cut(s) 340, 365, 378
BstV1I GCAGC 4 cut(s) 265, 359, 799, 886
BstX2I RGATCY 2 cut(s) 370, 435
BstYI RGATCY 2 cut(s) 370, 435
Bsu15I ATCGAT 2 cut(s) 276, 746
BsuRI GGCC 5 cut(s) 5, 266, 303, 446, 630
BsuTUI ATCGAT 2 cut(s) 276, 746
BtgI CCRYGG 1 cut(s) 407
BtsCI GGATG 2 cut(s) 466, 551
BtsI GCAGTG 2 cut(s) 288, 797
BtsIMutI CAGTG 4 cut(s) 51, 288, 700, 797
Cac8I GCNNGC 2 cut(s) 60, 201
CciI TCATGA 1 cut(s) 358
CfoI GCGC 2 cut(s) 508, 879
Cfr13I GGNCC 2 cut(s) 265, 529
ClaI ATCGAT 2 cut(s) 276, 746
Csp6I GTAC 1 cut(s) 959
CviAII CATG 5 cut(s) 7, 173, 359, 761, 773
CviQI GTAC 1 cut(s) 959
DpnI GATC 9 cut(s) 171, 354, 372, 437, 475, 644, 669, 779, 894
DpnII GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
DraI TTTAAA 1 cut(s) 736
EaeI YGGCCR 2 cut(s) 3, 628
Eco130I CCWWGG 2 cut(s) 160, 532
Eco32I GATATC 1 cut(s) 725
Eco47I GGWCC 1 cut(s) 529
Eco88I CYCGRG 1 cut(s) 636
EcoNI CCTNNNNNAGG 1 cut(s) 807
EcoRII CCWGG 2 cut(s) 340, 365
EcoRV GATATC 1 cut(s) 725
EcoT14I CCWWGG 2 cut(s) 160, 532
ErhI CCWWGG 2 cut(s) 160, 532
Esp3I CGTCTC 2 cut(s) 291, 566
FaeI CATG 5 cut(s) 10, 176, 362, 764, 776
FaiI YATR 7 cut(s) 8, 174, 360, 516, 729, 762, 774
FaqI GGGAC 1 cut(s) 222
FatI CATG 5 cut(s) 6, 172, 358, 760, 772
FbaI TGATCA 1 cut(s) 169
Fnu4HI GCNGC 5 cut(s) 254, 348, 653, 788, 875
FokI GGATG 2 cut(s) 473, 558
Fsp4HI GCNGC 5 cut(s) 254, 348, 653, 788, 875
FspBI CTAG 3 cut(s) 161, 809, 846
GlaI GCGC 2 cut(s) 507, 878
GluI GCNGC 5 cut(s) 254, 348, 653, 788, 875
GsaI CCCAGC 3 cut(s) 207, 422, 512
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 5 cut(s) 5, 266, 303, 446, 630
HapII CCGG 3 cut(s) 379, 677, 704
HhaI GCGC 2 cut(s) 508, 879
Hin1II CATG 5 cut(s) 10, 176, 362, 764, 776
Hin6I GCGC 2 cut(s) 506, 877
HinP1I GCGC 2 cut(s) 506, 877
HinfI GANTC 3 cut(s) 362, 743, 932
HpaII CCGG 3 cut(s) 379, 677, 704
HphI GGTGA 2 cut(s) 652, 902
Hpy166II GTNNAC 3 cut(s) 529, 555, 923
Hpy188I TCNGA 6 cut(s) 125, 451, 478, 500, 619, 667
Hpy188III TCNNGA 3 cut(s) 22, 223, 359
Hpy8I GTNNAC 3 cut(s) 529, 555, 923
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 4 cut(s) 57, 173, 267, 571
HpyCH4III ACNGT 3 cut(s) 408, 607, 927
HpyCH4V TGCA 4 cut(s) 176, 281, 347, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 793
Hsp92II CATG 5 cut(s) 10, 176, 362, 764, 776
HspAI GCGC 2 cut(s) 506, 877
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
Lsp1109I GCAGC 4 cut(s) 265, 359, 799, 886
LweI GCATC 2 cut(s) 268, 536
MaeI CTAG 3 cut(s) 161, 809, 846
MaeIII GTNAC 4 cut(s) 155, 235, 389, 913
MalI GATC 9 cut(s) 171, 354, 372, 437, 475, 644, 669, 779, 894
MboI GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
MboII GAAGA 3 cut(s) 79, 365, 850
MflI RGATCY 2 cut(s) 370, 435
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 25, 95, 521, 737
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 941
MmeI TCCRAC 2 cut(s) 567, 909
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 74, 429, 735
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 379, 677, 704
MspR9I CCNGG 3 cut(s) 342, 367, 380
Mva1269I GAATGC 1 cut(s) 773
MvaI CCWGG 2 cut(s) 342, 367
MwoI GCNNNNNNNGC 2 cut(s) 189, 793
NciI CCSGG 1 cut(s) 380
NdeII GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
NlaIII CATG 5 cut(s) 10, 176, 362, 764, 776
NmuCI GTSAC 1 cut(s) 389
PagI TCATGA 1 cut(s) 358
PctI GAATGC 1 cut(s) 773
PfeI GAWTC 2 cut(s) 362, 743
PkrI GCNGC 5 cut(s) 255, 349, 654, 789, 876
PleI GAGTC 1 cut(s) 940
PpsI GAGTC 1 cut(s) 940
Psp6I CCWGG 2 cut(s) 340, 365
PspFI CCCAGC 3 cut(s) 203, 418, 508
PspGI CCWGG 2 cut(s) 340, 365
PspPI GGNCC 2 cut(s) 265, 529
PsuI RGATCY 2 cut(s) 370, 435
RsaI GTAC 1 cut(s) 960
RsaNI GTAC 1 cut(s) 959
RseI CAYNNNNRTG 1 cut(s) 11
SaqAI TTAA 3 cut(s) 74, 429, 735
SatI GCNGC 5 cut(s) 254, 348, 653, 788, 875
Sau3AI GATC 9 cut(s) 169, 352, 370, 435, 473, 642, 667, 777, 892
Sau96I GGNCC 2 cut(s) 265, 529
SchI GAGTC 1 cut(s) 941
ScrFI CCNGG 3 cut(s) 342, 367, 380
SetI ASST 8 cut(s) 162, 346, 459, 496, 534, 582, 587, 734
SfaNI GCATC 2 cut(s) 268, 536
SinI GGWCC 1 cut(s) 529
SmiMI CAYNNNNRTG 1 cut(s) 11
Sse9I AATT 4 cut(s) 25, 95, 521, 737
SsiI CCGC 3 cut(s) 310, 518, 653
SspMI CTAG 3 cut(s) 161, 809, 846
StyD4I CCNGG 3 cut(s) 340, 365, 378
StyI CCWWGG 2 cut(s) 160, 532
TaaI ACNGT 3 cut(s) 408, 607, 927
TaqI TCGA 4 cut(s) 31, 141, 276, 746
TasI AATT 4 cut(s) 25, 95, 521, 737
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 2 cut(s) 362, 743
Tru1I TTAA 3 cut(s) 74, 429, 735
Tru9I TTAA 3 cut(s) 74, 429, 735
TscAI CASTG 4 cut(s) 58, 288, 700, 797
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 4 cut(s) 253, 347, 787, 874
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 23, 375
TspGWI ACGGA 1 cut(s) 558
TspRI CASTG 4 cut(s) 58, 288, 700, 797
VpaK11BI GGWCC 1 cut(s) 529
XagI CCTNNNNNAGG 1 cut(s) 807
XapI RAATTY 1 cut(s) 737
XmaJI CCTAGG 1 cut(s) 160
XspI CTAG 3 cut(s) 161, 809, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.