RLG00000020130

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004044
Physical Location & Seq
Forward (+)
61399570 .. 61410674
11105 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020130

Sequence Viewer

Length: 5655 bp
ATGGACTCCATTCTAAACTCGGCGATCGAAGAAATCTGCTCGCACCTCCAAGACGGCCTCTCTCTCCAGACCCTCTGGTCCCGACTCTCCTCCTGCAACCTCGACCTCTCTCCCACCCTCAAACAATCCCTATGGGACGCCCTCCGCTCCGTCCCCACTGTCAAATTCCGCGCCCAAAATGACCATTACGCCCCCACCGACCCTTCCATTCACTCCTTCCAAGACGCCGAGAAGCTCAATTTGAAGCTCGTCGCCGATGAGGCCCTCCGGAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAATGCCAACCTGTCTGCCGTTCAACGCCACACTCTCGAACGCGTCGTCACGGCTAGGGGGAATGGGATTACACAAACTCAGCTTGCTAAGGAGCTTGGCATTGAGGGGAGAAATTTTCATTATGCCGTGAAGAACCTTGAATGTCAAGGGTTACTTGTACGGCAATCTGCGCTGTTAAGGACAAAAGAAGCTGGTGATGAGGGGGAGCCGAGGAACAATCCGAGTGTGACAACGACCAATATGTTGTACTTGTATCGACATGCAAAGCATTTAAGTGCGCAACAAAAGATTGAGATCACAAAGGAAGAGAGGGCTAAGGAGAGTTTTGTAAATGTAAAAGAAAGACCTGCAAGTGGAGATGGGTTTGCTGGAAATGTGCTCGTAAAGGACTATCTACCGGCAATGAAAGCAGTCTGTGATAAACTTGAAGAAGCCAATGGCAAGGTTCTTGTCGTCTCGGATATTAAGAAGGAACTTGGCTACAGTAAAACATCGGGGCATAGAGCTTGGAGAAATATTTGTCACAGGCTGAAAGCTGCTCATCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGGAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAATGAAAGTTGAGCCAAGAGAAAATGTACATGTAGATGAGGATTTTGTTGAAGAACAACAAGTGAAATTCGGAAAGAAATGTAAAATCAGTGACCAGCTTGTAGAGCTTCCTATTGAGCATCAAATTTATGAATTAATAGATGTTGCAGGATCAGAGGGGTTGACCAGGAATGAGGTTATGGAGAGGCTTGGAATTGACAACAAGAAAAATTATACCCGCTTTGTTAGTATGTGCTCTAGATTTGATATGAGTCTGCAACCAGAAATGCACAAAAAAGCCGTGGCATATCGATTTCGGACATCTGGGAAACACAAGTCTGAATCAACTAATGCATTTCTTCGAAAATCAAAAGATGTCAATGACAGTAAAATTTCTAGTCTAAATGTTGGGAGCGTAGATGCTCCGAGATCAGATCAATTTCAAACTGGCTCAGTGTCAGATTGTTGGTCTCTGAACGGTGATACTGCTGGCCCTGAAAATATGAATAACATAGAGACTAATACTGACCCTTCTGCTGGATCTCTTGGGCGTAATGAGTTTGACGATATGCCTGAAACTTCCCAACAGTTACTTCTTGGACCAAAAGATACTACATCTGATTCTCAAGTTAGTTTAGTGAGCGCAGGAGTGGAACCAAATGGCGCCTTATCAGAAATACCAGCTGCTTTGTCAAAGCCACTTACTAAAGGCTCAGATCCAAGGTATCCATGTATGTCTTTGACCGTGGATAACACTCGAAGGGAAAAGAGGATACTTGAACGGCTAGAGGGTGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGACAAGTGCACAGCAACTGACAGGAAAACAATTGACCGAATTTTACAAAAACTTCAACAGCTAGGGCACTGCAAGTGTATAGATATTAGCGTCCCTGTCGTCACAAATTTGGGCCGTACCCGAACCACTGTAGTGGTTCTGCACCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCTTGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAATGGATCAGTTCCTGTACTAGAAGATGTTCAGAGAACTCAGACCCATGTTAGTGCACAGCGACAAACACTAAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTGCTGCATAGTTTCCTGTGGGATTACCTGTATGGTTCGTCCGGCTCTAATGATGCTTTATCATTTCGAAAAGATGTTATTGAGCCAAGAGATCCTCACAGTACTAGAAAATTGTTTTCGCTAGAAGCAACAATGAAGGCTATTCCAGTTGAGTTGTTTCTACAAGTTGCGGGATCCACCAAAAACTTTGAGGATATGATTGAGAAATGTAAGAGGGGTTTATGTCTCTCTGATCTCTCTCGCGAAGAATACAAGTCTCTGATGGATACCCATGCAACTGGAAGGCTCTCATTGGTGATTGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGATCACCACTCAGAAAATGGATTCCAAGTTCCCCCTCCCATGATTTCTGCATATGCATTGGAACTTAAGCCTTATGTAGAGGAACCTGTTTCAAAAGATGCGATATATTTGAGTTTTGGATCACGTGATCTTCGTCCACGAATCAGACATGATTTTTCCCTATCAAGCAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCAGACTCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAGCGTGATGAGCTCTTAAAGCGTGTTGTGAAGGATGATCCAAGCGAAAAGCTTTCATTCAAAGAATGTGGAAAAATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACACAGATGAAGTTCAATCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATGATGAGGTAACTGGACAGCTGGAAGAGCAGACACATCCCACAGTATCTGATACTGTGGAACAATTAGAAGAACTAAATCTCTTGGTCACTTCTTATGAGCATGATTCACATTTGCAAGCATTGGACAATTGTCTAGAAACTGGGCAGGACCCTGAACCAAATGAAGATAATGAGGGGTGTCATTCAATTATTACCAAGGGTTCCTTTTCAAAGTTGAAGCCACTACGTCCTTCTAAAAAAAACAAGTTGCACTCAACACGTCAACGAAGGTTTTCATGGACAGAGGAGGCTGATAGGCAGTTGATTATCCAATATGTAAAACATCGTGCAACTCTTGGGGCAAAGATTCACCGGATAAATTGGGCTTCAGTTACTGACCTCCCAGCACCTCCTATTGCCTGCATGAAAAGAATGGCATCCCTGAAAAGTAACAAAAAGTTCAGATTAGCAGTAATGAGGCTTTGTAATATTCTCAGTGAACGATATGCAAAGATCTTGGAAAAGACTCAGAACAGGTCACTGAACAAAGATGATTGCAATCTGCTTTTGCGAGATTCAATAGGTGAAGGTCATGACAGCAAACTTCCCAATATCAGTGATCACAATCCAGAGACAGGTCTTCAGGAAGAACCATGGGATGATTTTGATGACACTTACGTTAAAAAGTCCCTTGAGGAGGTACTTCACCACAAGAGGTTGGCCAAATTTGAGGCCTCCAAAAGAGTTGGATCTACCGGTGAGGACTGGTCAGATCTTAATACAAGTGAATGTGATCCCCAGGAATCTGAATTAATTGCGTCAACTGCTCCATATGAAGATGTTCAGAATCATGGTGGCAGAGAAAAAATTTCGGCACAAAGATCTTGCTATCAACACCTTGATGAAAAATACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACGCAAGTCTACAAATCATTAGCTGTCTCTAATGCTGTAGAGCTGTTTAAGCTTGTTTTCCTCAGTACCTCGACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCATTCTACGGCGGTACTCAGAGTGTGATCTATTTGCAGCTTTCAACTATCTTAGAGATAAAAAGTTCATGGTTGGAGGTAATGGCAGTCAAAAATTTTCTCTCTCACAACAGTTCTTGCATAATACTTCCGCATCAGCATTTCCAATTAATTCTGGGAAGAGAGCTACTAAATTCGCCCATTGGCTCTACGAAAAAGATAAAGATCTTATGGAAGGGGGGATTGATCTCTCCACAGATTTACAGTGTGGGGACATATTCCATTTGTTTGCTCTAGTTTCTTCAGGTGAACTGTCAATTTGTCCATGCTTGCCAGATGAAGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCAGATATCAATGAACGTTTAGATGATGAAAAAACTAAAAAGCTGAAATCATTTGTGGCTGCAGAAGGTGAAATCATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCACGGTATTTGTAAGTCGTGCGGAGTTTTCTATAGCCAGTTCTATAGATTTGTTCAAGGAAGACAGTCCTATTGGTGACAAACATTTTGGGGGAAATCATCAACTAGAATGTACATCTGATCAGAGTAGCTTATCACATTCTGACTGTATGAAAGAAATCTTCAATTCAGGCAGTACTGTCCCTGTATTAGAGATTGGTTGCGAGTCACCTTGGGAAGGTATGGTGGCTTATGCCAAACATTTGTTGCCATTACATCCTGCTCAAGATCAATCAAGTCCCATCCAGCCTGAGGTCTTTAGGGCTGTTTACATTGCTATTCAGAAAGCTGGCGATCAAGGTCTGAGCATAGAAGAAGTTTCCCGAATAATGAATATCCCAGGAGAAAAGATGACTGATCTTATCATTGATGTACTTCAGACATTTGAACGAGTCTTAAAGGTCAATGCTTATGACTCTATTCGTGTTGTGGATTCTTTGTATCGTGGCAAGTACTTTATGACTTCAGTGTCTGGAATTAATCAAAAACTCGAACCGCCTTCCTGGAGAAAGCCACTAGGCGATGATGGCCATATACTCATCCACTCAGAAAATCGTGATAATGGTGCTGCTACTTCAGAGAGGGAGATAAATGCTGATGTCCATAAATTGACAATTCTTAATTTTCCTGAAGAGGTTGATGTGCCCTCATATGTAAAACAAATTAGCAGTGTTCCTGAAAGCTATAGGGAAGGAAAAGGAGGAGATGCTGAGGATGAATCTTCTAGGTCCTCTAATGATAGACTATGCATGCCTATATTTCCATGGATAAATGGTGATGGGACCACTAACAAGATTGTCTACAAGGGACTCAGGCGACGTGTGCTTGGGATTGTGATGCAAAATCCAGCTATATTAGAGGATGAAATTATTCGCCGGATGGATGTATTGAATCCCCAGAGCTGTAGAAAATTGTTGGAATTGATGGTTTTGGATAACCATCTTCATGTGAGGAAGATGCATCAAACTACGTGCACTGGGCCCCCACCAATTTTAGGTACCCTCCTTGGAAGCAGCTTCAAACCGTCAAAGCTGGTTTGTCGCGAGCACTATTTTGCAAATCCCATGAGTACATCCTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1885

Amino Acids

212.32

Weight (kDa)

6.59

Isoelectric Point (pI)

51.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 97 2.9e-33 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 108 - 191 7.2e-13 B-block binding subunit of TFIIIC
DUF7599 PF24538 231 - 313 2.1e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 332 - 416 7.3e-37 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 548 - 657 3.1e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 743 - 922 2.3e-82 Domain of unknown function (DUF7647)
DUF7645 PF24655 925 - 980 8.8e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2916
AarI CACCTGC 1 cut(s) 2887
AasI GACNNNNNNGTC 2 cut(s) 76, 5038
AatII GACGTC 1 cut(s) 2989
Acc16I TGCGCA 1 cut(s) 585
Acc36I ACCTGC 2 cut(s) 661, 2887
Acc65I GGTACC 1 cut(s) 5567
AccB1I GGYRCC 2 cut(s) 1565, 5567
AccBSI CCGCTC 1 cut(s) 147
AccI GTMKAC 4 cut(s) 297, 2983, 3969, 5370
AccII CGCG 4 cut(s) 171, 348, 2376, 5613
AccIII TCCGGA 1 cut(s) 267
AclI AACGTT 1 cut(s) 4441
AcoI YGGCCR 2 cut(s) 3738, 5098
AcuI CTGAAG 8 cut(s) 3390, 3644, 4329, 4419, 4931, 5019, 5130, 5220
AcvI CACGTG 1 cut(s) 2597
AcyI GRCGYC 5 cut(s) 138, 225, 1566, 2926, 2986
AflII CTTAAG 1 cut(s) 2537
AflIII ACRYGT 4 cut(s) 346, 952, 3296, 5389
AgeI ACCGGT 1 cut(s) 3773
AjiI CACGTC 2 cut(s) 3299, 5390
AjnI CCWGG 6 cut(s) 1088, 2722, 2889, 3816, 4909, 5072
AjuI GAANNNNNNNTTGG 2 cut(s) 1479, 1511
AleI CACNNNNGTG 1 cut(s) 1880
AloI GAACNNNNNNTCC 2 cut(s) 2484, 2516
Alw21I GWGCWC 8 cut(s) 687, 1160, 1760, 1982, 2062, 2805, 5546, 5619
Alw26I GTCTC 8 cut(s) 766, 1377, 1412, 1739, 2363, 2394, 3644, 3991
Alw44I GTGCAC 3 cut(s) 1756, 2058, 5542
AlwNI CAGNNNCTG 5 cut(s) 1766, 2018, 3086, 3413, 5042
Ama87I CYCGRG 1 cut(s) 4525
Aor13HI TCCGGA 1 cut(s) 267
AoxI GGCC 9 cut(s) 55, 261, 283, 1393, 1861, 3738, 3750, 5098, 5549
ApaI GGGCCC 1 cut(s) 5553
ApaLI GTGCAC 3 cut(s) 1756, 2058, 5542
ArsI GACNNNNNNTTYG 4 cut(s) 336, 368, 4601, 4633
AseI ATTAAT 4 cut(s) 1058, 3830, 4212, 5049
AsiGI ACCGGT 1 cut(s) 3773
Asp700I GAANNNNTTC 5 cut(s) 275, 2031, 3310, 3858, 5439
Asp718I GGTACC 1 cut(s) 5567
AspLEI GCGC 5 cut(s) 173, 478, 586, 1547, 1568
AsuC2I CCSGG 2 cut(s) 4526, 4527
AsuII TTCGAA 3 cut(s) 1264, 2200, 2461
AvaI CYCGRG 1 cut(s) 4525
AvaII GGWCC 5 cut(s) 78, 1502, 3187, 5298, 5352
AxyI CCTNAGG 1 cut(s) 4821
BaeGI GKGCMC 6 cut(s) 1760, 1818, 2062, 5217, 5546, 5553
BalI TGGCCA 2 cut(s) 3740, 5100
BamHI GGATCC 1 cut(s) 2306
BanI GGYRCC 2 cut(s) 1565, 5567
BanII GRGCYC 2 cut(s) 2805, 5553
BbrPI CACGTG 1 cut(s) 2597
BbsI GAAGAC 2 cut(s) 3650, 4599
Bbv12I GWGCWC 8 cut(s) 687, 1160, 1760, 1982, 2062, 2805, 5546, 5619
BbvCI CCTCAGC 1 cut(s) 5280
BceAI ACGGC 9 cut(s) 70, 308, 372, 416, 482, 1187, 1700, 1848, 4088
BcgI CGANNNNNNTGC 2 cut(s) 2885, 2919
BciT130I CCWGG 6 cut(s) 1090, 2724, 2891, 3818, 4911, 5074
BciVI GTATCC 3 cut(s) 1638, 1668, 2392
BclI TGATCA 3 cut(s) 2473, 3637, 4651
BcnI CCSGG 2 cut(s) 4526, 4527
BcoDI GTCTC 8 cut(s) 766, 1377, 1412, 1739, 2363, 2394, 3644, 3991
BfmI CTRYAG 9 cut(s) 787, 1878, 2691, 3996, 4485, 4563, 4575, 5254, 5473
BfoI RGCGCY 1 cut(s) 1569
BfrI CTTAAG 1 cut(s) 2537
BfuAI ACCTGC 2 cut(s) 661, 2887
BfuI GTATCC 3 cut(s) 1638, 1668, 2392
BglI GCCNNNNNGGC 1 cut(s) 260
BglII AGATCT 5 cut(s) 3002, 3531, 3790, 3899, 4267
BlpI GCTNAGC 2 cut(s) 1716, 2790
BmcAI AGTACT 3 cut(s) 2236, 4708, 5024
Bme1390I CCNGG 8 cut(s) 1090, 2724, 2891, 3818, 4526, 4527, 4911, 5074
Bme18I GGWCC 5 cut(s) 78, 1502, 3187, 5298, 5352
BmeT110I CYCGRG 1 cut(s) 4525
BmgBI CACGTC 2 cut(s) 3299, 5390
BmrFI CCNGG 8 cut(s) 1090, 2724, 2891, 3818, 4526, 4527, 4911, 5074
BmrI ACTGGG 2 cut(s) 3189, 5556
BmuI ACTGGG 2 cut(s) 3189, 5556
BoxI GACNNNNGTC 1 cut(s) 3168
BpiI GAAGAC 2 cut(s) 3650, 4599
BplI GAGNNNNNCTC 2 cut(s) 1897, 1929
BpmI CTGGAG 3 cut(s) 50, 2912, 5095
Bpu10I CCTNAGC 3 cut(s) 393, 621, 5280
Bpu1102I GCTNAGC 2 cut(s) 1716, 2790
Bpu14I TTCGAA 3 cut(s) 1264, 2200, 2461
BpuEI CTTGAG 4 cut(s) 1512, 2683, 3731, 4779
BpuMI CCSGG 2 cut(s) 4526, 4527
Bsa29I ATCGAT 1 cut(s) 1213
BsaAI YACGTR 2 cut(s) 2597, 5541
BsaBI GATNNNNATC 5 cut(s) 599, 3576, 3642, 4266, 5508
BsaHI GRCGYC 5 cut(s) 138, 225, 1566, 2926, 2986
BsaI GGTCTC 1 cut(s) 1377
BsaWI WCCGGW 3 cut(s) 267, 3390, 3773
BsaXI ACNNNNNCTCC 2 cut(s) 3939, 3969
Bse118I RCCGGY 2 cut(s) 703, 3773
Bse1I ACTGG 8 cut(s) 1354, 2279, 2416, 3055, 3184, 3788, 4569, 5551
Bse21I CCTNAGG 1 cut(s) 4821
Bse3DI GCAATG 2 cut(s) 714, 4842
Bse8I GATNNNNATC 5 cut(s) 599, 3576, 3642, 4266, 5508
BseAI TCCGGA 1 cut(s) 267
BseBI CCWGG 6 cut(s) 1090, 2724, 2891, 3818, 4911, 5074
BseCI ATCGAT 1 cut(s) 1213
BseJI GATNNNNATC 5 cut(s) 599, 3576, 3642, 4266, 5508
BseMI GCAATG 2 cut(s) 714, 4842
BseNI ACTGG 8 cut(s) 1354, 2279, 2416, 3055, 3184, 3788, 4569, 5551
BseRI GAGGAG 4 cut(s) 79, 3338, 3728, 5286
BseSI GKGCMC 6 cut(s) 1760, 1818, 2062, 5217, 5546, 5553
BseYI CCCAGC 1 cut(s) 3421
BsgI GTGCAG 1 cut(s) 1874
Bsh1236I CGCG 4 cut(s) 171, 348, 2376, 5613
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 9 cut(s) 57, 263, 285, 1395, 1863, 3740, 3752, 5100, 5551
BshNI GGYRCC 2 cut(s) 1565, 5567
BshTI ACCGGT 1 cut(s) 3773
BshVI ATCGAT 1 cut(s) 1213
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 8 cut(s) 687, 1160, 1760, 1982, 2062, 2805, 5546, 5619
BsiHKCI CYCGRG 1 cut(s) 4525
BsiSI CCGG 7 cut(s) 268, 704, 2175, 3391, 3774, 4526, 5446
BsmAI GTCTC 8 cut(s) 766, 1377, 1412, 1739, 2363, 2394, 3644, 3991
BsmBI CGTCTC 2 cut(s) 766, 1739
BsmI GAATGC 1 cut(s) 4065
BsnI GGCC 9 cut(s) 57, 263, 285, 1395, 1863, 3740, 3752, 5100, 5551
Bso31I GGTCTC 1 cut(s) 1377
BsoBI CYCGRG 1 cut(s) 4525
Bsp119I TTCGAA 3 cut(s) 1264, 2200, 2461
Bsp120I GGGCCC 1 cut(s) 5549
Bsp13I TCCGGA 1 cut(s) 267
Bsp1407I TGTACA 2 cut(s) 949, 4643
Bsp1720I GCTNAGC 2 cut(s) 1716, 2790
Bsp19I CCATGG 2 cut(s) 3671, 5333
Bsp68I TCGCGA 2 cut(s) 2376, 5613
BspANI GGCC 9 cut(s) 57, 263, 285, 1395, 1863, 3740, 3752, 5100, 5551
BspDI ATCGAT 1 cut(s) 1213
BspEI TCCGGA 1 cut(s) 267
BspFNI CGCG 4 cut(s) 171, 348, 2376, 5613
BspHI TCATGA 3 cut(s) 1936, 2737, 3610
BspMAI CTGCAG 2 cut(s) 2695, 4489
BspMI ACCTGC 2 cut(s) 661, 2887
BspQI GCTCTTC 1 cut(s) 3057
BspT104I TTCGAA 3 cut(s) 1264, 2200, 2461
BspT107I GGYRCC 2 cut(s) 1565, 5567
BspTI CTTAAG 1 cut(s) 2537
BspTNI GGTCTC 1 cut(s) 1377
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 714, 4842
BsrFI RCCGGY 2 cut(s) 703, 3773
BsrGI TGTACA 2 cut(s) 949, 4643
BsrI ACTGG 8 cut(s) 1354, 2279, 2416, 3055, 3184, 3788, 4569, 5551
BssAI RCCGGY 2 cut(s) 703, 3773
BssNI GRCGYC 5 cut(s) 138, 225, 1566, 2926, 2986
BssT1I CCWWGG 6 cut(s) 1622, 3234, 3671, 4742, 5333, 5575
Bst2UI CCWGG 6 cut(s) 1090, 2724, 2891, 3818, 4911, 5074
Bst6I CTCTTC 5 cut(s) 606, 2993, 3057, 4217, 5196
BstACI GRCGYC 5 cut(s) 138, 225, 1566, 2926, 2986
BstAFI CTTAAG 1 cut(s) 2537
BstAUI TGTACA 2 cut(s) 949, 4643
BstBAI YACGTR 2 cut(s) 2597, 5541
BstBI TTCGAA 3 cut(s) 1264, 2200, 2461
BstDSI CCRYGG 4 cut(s) 1203, 1647, 3671, 5333
BstENI CCTNNNNNAGG 3 cut(s) 2894, 3713, 4746
BstFNI CGCG 4 cut(s) 171, 348, 2376, 5613
BstH2I RGCGCY 1 cut(s) 1569
BstHHI GCGC 5 cut(s) 173, 478, 586, 1547, 1568
BstMAI GTCTC 8 cut(s) 766, 1377, 1412, 1739, 2363, 2394, 3644, 3991
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 6 cut(s) 1090, 2724, 2891, 3818, 4911, 5074
BstNSI RCATGY 3 cut(s) 569, 956, 5323
BstPAI GACNNNNGTC 1 cut(s) 3168
BstSCI CCNGG 8 cut(s) 1088, 2722, 2889, 3816, 4524, 4525, 4909, 5072
BstSFI CTRYAG 9 cut(s) 787, 1878, 2691, 3996, 4485, 4563, 4575, 5254, 5473
BstSLI GKGCMC 6 cut(s) 1760, 1818, 2062, 5217, 5546, 5553
BstUI CGCG 4 cut(s) 171, 348, 2376, 5613
BstV2I GAAGAC 2 cut(s) 3650, 4599
BstXI CCANNNNNNTGG 2 cut(s) 1882, 2411
Bsu15I ATCGAT 1 cut(s) 1213
Bsu36I CCTNAGG 1 cut(s) 4821
BsuI GTATCC 3 cut(s) 1638, 1668, 2392
BsuRI GGCC 9 cut(s) 57, 263, 285, 1395, 1863, 3740, 3752, 5100, 5551
BsuTUI ATCGAT 1 cut(s) 1213
BtgI CCRYGG 4 cut(s) 1203, 1647, 3671, 5333
BtgZI GCGATG 1 cut(s) 5106
BtrI CACGTC 2 cut(s) 3299, 5390
BtsI GCAGTG 2 cut(s) 1816, 5245
BtuMI TCGCGA 2 cut(s) 2376, 5613
BveI ACCTGC 2 cut(s) 661, 2887
CaiI CAGNNNCTG 5 cut(s) 1766, 2018, 3086, 3413, 5042
CciI TCATGA 3 cut(s) 1936, 2737, 3610
CfoI GCGC 5 cut(s) 173, 478, 586, 1547, 1568
Cfr10I RCCGGY 2 cut(s) 703, 3773
Cfr9I CCCGGG 1 cut(s) 4525
ClaI ATCGAT 1 cut(s) 1213
CseI GACGC 7 cut(s) 146, 233, 337, 875, 1828, 2915, 3825
CspAI ACCGGT 1 cut(s) 3773
CspCI CAANNNNNGTGG 2 cut(s) 2839, 2874
DinI GGCGCC 1 cut(s) 1567
DrdI GACNNNNNNGTC 2 cut(s) 76, 5038
DseDI GACNNNNNNGTC 2 cut(s) 76, 5038
EaeI YGGCCR 2 cut(s) 3738, 5098
Eam1104I CTCTTC 5 cut(s) 606, 2993, 3057, 4217, 5196
EarI CTCTTC 5 cut(s) 606, 2993, 3057, 4217, 5196
Ecl136II GAGCTC 1 cut(s) 2803
Eco130I CCWWGG 6 cut(s) 1622, 3234, 3671, 4742, 5333, 5575
Eco147I AGGCCT 1 cut(s) 3752
Eco24I GRGCYC 2 cut(s) 2805, 5553
Eco31I GGTCTC 1 cut(s) 1377
Eco32I GATATC 1 cut(s) 4432
Eco47I GGWCC 5 cut(s) 78, 1502, 3187, 5298, 5352
Eco53kI GAGCTC 1 cut(s) 2803
Eco57I CTGAAG 8 cut(s) 3390, 3644, 4329, 4419, 4931, 5019, 5130, 5220
Eco72I CACGTG 1 cut(s) 2597
Eco81I CCTNAGG 1 cut(s) 4821
Eco88I CYCGRG 1 cut(s) 4525
EcoICRI GAGCTC 1 cut(s) 2803
EcoNI CCTNNNNNAGG 3 cut(s) 2894, 3713, 4746
EcoO109I RGGNCCY 4 cut(s) 262, 3187, 5298, 5550
EcoRII CCWGG 6 cut(s) 1088, 2722, 2889, 3816, 4909, 5072
EcoRV GATATC 1 cut(s) 4432
EcoT14I CCWWGG 6 cut(s) 1622, 3234, 3671, 4742, 5333, 5575
EcoT22I ATGCAT 4 cut(s) 1258, 2530, 5321, 5532
EcoT38I GRGCYC 2 cut(s) 2805, 5553
EgeI GGCGCC 1 cut(s) 1567
EheI GGCGCC 1 cut(s) 1567
ErhI CCWWGG 6 cut(s) 1622, 3234, 3671, 4742, 5333, 5575
Esp3I CGTCTC 2 cut(s) 766, 1739
FalI AAGNNNNNCTT 4 cut(s) 444, 476, 3227, 3259
FauI CCCGC 3 cut(s) 1148, 1986, 2296
FauNDI CATATG 3 cut(s) 2524, 3850, 5221
FbaI TGATCA 3 cut(s) 2473, 3637, 4651
FblI GTMKAC 4 cut(s) 297, 2983, 3969, 5370
FriOI GRGCYC 2 cut(s) 2805, 5553
FspI TGCGCA 1 cut(s) 585
GlaI GCGC 5 cut(s) 172, 477, 585, 1546, 1567
GsaI CCCAGC 1 cut(s) 3425
GsuI CTGGAG 3 cut(s) 50, 2912, 5095
HaeII RGCGCY 1 cut(s) 1569
HaeIII GGCC 9 cut(s) 57, 263, 285, 1395, 1863, 3740, 3752, 5100, 5551
HapII CCGG 7 cut(s) 268, 704, 2175, 3391, 3774, 4526, 5446
HgaI GACGC 7 cut(s) 146, 233, 337, 875, 1828, 2915, 3825
HhaI GCGC 5 cut(s) 173, 478, 586, 1547, 1568
Hin1I GRCGYC 5 cut(s) 138, 225, 1566, 2926, 2986
Hin6I GCGC 5 cut(s) 171, 476, 584, 1545, 1566
HinP1I GCGC 5 cut(s) 171, 476, 584, 1545, 1566
HincII GTYRAC 5 cut(s) 298, 1086, 2930, 3302, 3840
HindII GTYRAC 5 cut(s) 298, 1086, 2930, 3302, 3840
HindIII AAGCTT 3 cut(s) 2840, 3935, 4010
HpaII CCGG 7 cut(s) 268, 704, 2175, 3391, 3774, 4526, 5446
Hpy99I CGWCG 6 cut(s) 254, 353, 2448, 2991, 4512, 5391
Hsp92I GRCGYC 5 cut(s) 138, 225, 1566, 2926, 2986
HspAI GCGC 5 cut(s) 171, 476, 584, 1545, 1566
KasI GGCGCC 1 cut(s) 1565
Kpn2I TCCGGA 1 cut(s) 267
KpnI GGTACC 1 cut(s) 5571
Ksp22I TGATCA 3 cut(s) 2473, 3637, 4651
LguI GCTCTTC 1 cut(s) 3057
LmnI GCTCC 7 cut(s) 152, 397, 511, 1314, 1330, 2893, 3850
MbiI CCGCTC 1 cut(s) 147
MfeI CAATTG 2 cut(s) 1779, 3166
MlsI TGGCCA 2 cut(s) 3740, 5100
MluI ACGCGT 1 cut(s) 346
MluNI TGGCCA 2 cut(s) 3740, 5100
Mly113I GGCGCC 1 cut(s) 1566
MlyI GAGTC 9 cut(s) 78, 293, 1183, 2690, 3538, 4745, 4971, 4979, 5373
MmeI TCCRAC 3 cut(s) 3745, 4117, 5466
Mox20I TGGCCA 2 cut(s) 3740, 5100
Mph1103I ATGCAT 4 cut(s) 1258, 2530, 5321, 5532
MroI TCCGGA 1 cut(s) 267
MroXI GAANNNNTTC 5 cut(s) 275, 2031, 3310, 3858, 5439
MscI TGGCCA 2 cut(s) 3740, 5100
MslI CAYNNNNRTG 8 cut(s) 579, 957, 1880, 1987, 2055, 2937, 3918, 5514
Msp20I TGGCCA 2 cut(s) 3740, 5100
MspA1I CMGCKG 3 cut(s) 1586, 2786, 3058
MspCI CTTAAG 1 cut(s) 2537
MspI CCGG 7 cut(s) 268, 704, 2175, 3391, 3774, 4526, 5446
MspR9I CCNGG 8 cut(s) 1090, 2724, 2891, 3818, 4526, 4527, 4911, 5074
MunI CAATTG 2 cut(s) 1779, 3166
Mva1269I GAATGC 1 cut(s) 4065
MvaI CCWGG 6 cut(s) 1090, 2724, 2891, 3818, 4911, 5074
MvnI CGCG 4 cut(s) 171, 348, 2376, 5613
NarI GGCGCC 1 cut(s) 1566
NciI CCSGG 2 cut(s) 4526, 4527
NcoI CCATGG 2 cut(s) 3671, 5333
NdeI CATATG 3 cut(s) 2524, 3850, 5221
NmeAIII GCCGAG 2 cut(s) 253, 540
NmuCI GTSAC 9 cut(s) 352, 532, 827, 1013, 1849, 3124, 3555, 4607, 4737
NruI TCGCGA 2 cut(s) 2376, 5613
NsbI TGCGCA 1 cut(s) 585
NsiI ATGCAT 4 cut(s) 1258, 2530, 5321, 5532
NspI RCATGY 3 cut(s) 569, 956, 5323
NspV TTCGAA 3 cut(s) 1264, 2200, 2461
OliI CACNNNNGTG 1 cut(s) 1880
PaeI GCATGC 1 cut(s) 5323
PagI TCATGA 3 cut(s) 1936, 2737, 3610
PaqCI CACCTGC 1 cut(s) 2887
PceI AGGCCT 1 cut(s) 3752
PciI ACATGT 1 cut(s) 952
PciSI GCTCTTC 1 cut(s) 3057
PcsI WCGNNNNNNNCGW 4 cut(s) 195, 348, 2602, 2986
PctI GAATGC 1 cut(s) 4065
PdmI GAANNNNTTC 5 cut(s) 275, 2031, 3310, 3858, 5439
PflFI GACNNNGTC 1 cut(s) 302
PfoI TCCNGGA 1 cut(s) 5072
PinAI ACCGGT 1 cut(s) 3773
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 9 cut(s) 78, 293, 1182, 2690, 3538, 4744, 4970, 4979, 5373
PluTI GGCGCC 1 cut(s) 1569
PmaCI CACGTG 1 cut(s) 2597
PmlI CACGTG 1 cut(s) 2597
PpsI GAGTC 9 cut(s) 78, 293, 1182, 2690, 3538, 4744, 4970, 4979, 5373
Ppu21I YACGTR 2 cut(s) 2597, 5541
PpuMI RGGWCCY 2 cut(s) 3187, 5298
PscI ACATGT 1 cut(s) 952
PshAI GACNNNNGTC 1 cut(s) 3168
PshBI ATTAAT 4 cut(s) 1058, 3830, 4212, 5049
PsiI TTATAA 1 cut(s) 2916
Psp124BI GAGCTC 1 cut(s) 2805
Psp1406I AACGTT 1 cut(s) 4441
Psp5II RGGWCCY 2 cut(s) 3187, 5298
Psp6I CCWGG 6 cut(s) 1088, 2722, 2889, 3816, 4909, 5072
PspCI CACGTG 1 cut(s) 2597
PspFI CCCAGC 1 cut(s) 3421
PspGI CCWGG 6 cut(s) 1088, 2722, 2889, 3816, 4909, 5072
PspOMI GGGCCC 1 cut(s) 5549
PspPPI RGGWCCY 2 cut(s) 3187, 5298
PstI CTGCAG 2 cut(s) 2695, 4489
PstNI CAGNNNCTG 5 cut(s) 1766, 2018, 3086, 3413, 5042
PsyI GACNNNGTC 1 cut(s) 302
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 3 cut(s) 1586, 2786, 3058
RruI TCGCGA 2 cut(s) 2376, 5613
RseI CAYNNNNRTG 8 cut(s) 579, 957, 1880, 1987, 2055, 2937, 3918, 5514
SacI GAGCTC 1 cut(s) 2805
SalI GTCGAC 1 cut(s) 296
SapI GCTCTTC 1 cut(s) 3057
ScaI AGTACT 3 cut(s) 2236, 4708, 5024
SchI GAGTC 9 cut(s) 78, 293, 1183, 2690, 3538, 4745, 4971, 4979, 5373
ScrFI CCNGG 8 cut(s) 1090, 2724, 2891, 3818, 4526, 4527, 4911, 5074
SfcI CTRYAG 9 cut(s) 787, 1878, 2691, 3996, 4485, 4563, 4575, 5254, 5473
SfoI GGCGCC 1 cut(s) 1567
SfuI TTCGAA 3 cut(s) 1264, 2200, 2461
SinI GGWCC 5 cut(s) 78, 1502, 3187, 5298, 5352
SmaI CCCGGG 1 cut(s) 4527
SmiMI CAYNNNNRTG 8 cut(s) 579, 957, 1880, 1987, 2055, 2937, 3918, 5514
SmlI CTYRAG 5 cut(s) 1527, 2537, 2698, 3710, 4794
SmoI CTYRAG 5 cut(s) 1527, 2537, 2698, 3710, 4794
SphI GCATGC 1 cut(s) 5323
SseBI AGGCCT 1 cut(s) 3752
SspDI GGCGCC 1 cut(s) 1565
SspI AATATT 2 cut(s) 823, 3508
SstI GAGCTC 1 cut(s) 2805
StuI AGGCCT 1 cut(s) 3752
StyD4I CCNGG 8 cut(s) 1088, 2722, 2889, 3816, 4524, 4525, 4909, 5072
StyI CCWWGG 6 cut(s) 1622, 3234, 3671, 4742, 5333, 5575
TaqII GACCGA 1 cut(s) 1800
TatI WGTACW 9 cut(s) 552, 949, 2020, 2234, 4643, 4706, 4942, 5022, 5639
TauI GCSGC 1 cut(s) 2926
TseFI GTSAC 9 cut(s) 352, 532, 827, 1013, 1849, 3124, 3555, 4607, 4737
Tsp45I GTSAC 9 cut(s) 352, 532, 827, 1013, 1849, 3124, 3555, 4607, 4737
TspGWI ACGGA 3 cut(s) 139, 292, 1763
TspMI CCCGGG 1 cut(s) 4525
Tth111I GACNNNGTC 1 cut(s) 302
Vha464I CTTAAG 1 cut(s) 2537
VneI GTGCAC 3 cut(s) 1756, 2058, 5542
VpaK11BI GGWCC 5 cut(s) 78, 1502, 3187, 5298, 5352
VspI ATTAAT 4 cut(s) 1058, 3830, 4212, 5049
XagI CCTNNNNNAGG 3 cut(s) 2894, 3713, 4746
XbaI TCTAGA 2 cut(s) 1160, 3172
XceI RCATGY 3 cut(s) 569, 956, 5323
XcmI CCANNNNNNNNNTGG 1 cut(s) 2486
XmaI CCCGGG 1 cut(s) 4525
XmiI GTMKAC 4 cut(s) 297, 2983, 3969, 5370
XmnI GAANNNNTTC 5 cut(s) 275, 2031, 3310, 3858, 5439
ZraI GACGTC 1 cut(s) 2987
ZrmI AGTACT 3 cut(s) 2236, 4708, 5024
Zsp2I ATGCAT 4 cut(s) 1258, 2530, 5321, 5532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.