Rh2DG464300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004044
Physical Location & Seq
Forward (+)
67243915 .. 67255148
11234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG464300.1

Sequence Viewer

Length: 5658 bp
ATGGACTCCATTCTAAACTCAGCGATCGAAGAAATCTGCTCGTATCTCCAAAACGGCCTCTCTCTCCAGACCCTCTGGTCCCGACTCTCCTCCTCCTCCAACCTCGACCTCTCTCCCACCCTCAAGCAATCCCTATGGGACGCCCTCCGCTCCGTCCCCACTCTCAAATTCCGCGCCCAAAATGACCGTTACGCCCCCACCGACTCTTCCATTCACTCCTTCCAAGACGCCGAGAAGCTCAATTTGAAGCTCGTCGCCGATGAGGCCCTCCGGAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAATGCCAACCTGTCTGCCGTTCAACGCCACACTCTCGAACGCGTCGTCATGGCTAGGGGGAATGGGATTACACAAACTCAGCTTGCTAAGGAGCTTGGCATTGAGGGGAGAAACTTTCATTATGCTGTGAAGAACCTCGAATGTCAAGGTTTACTTGTACGGCAATCTGCGCTGTTAAGGACAAAAGAAGCTGGTGATGAGGGGGAGCCGAGGAACAATCCGAGTGTGACAACGACCAATATGTTGTACTTGTATCGACATGCAAAACATTTAAGCGCGCAACAAAAGATTGAGATCACAAAGGAAGAGAGGGCTAAGGAGAGTTTTGTAAATGTAAAAGAAAGCCCTGCAAGTGGAGATGGTTTTGCTGGAAATGTGCTCGTAAAGGACTATCTACCGGCAATGAAAGCAGTCTGTAATAAACTTGAAGAAGCCAATGGCAAGGTTCTTGTCGTTTCGGATATTAAGAAGGAACTTGGCTACAGTAAAACATCAGGGCATAGAGCTTGGAGAAATATTTGTCAGAGGTTGAAAGCTGCTCATCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGGAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAATGAAAGTTGAGCCAAGAGAAGTTGTACATGTAGATGAGGATTTTGTTGAAGAACAACAAATGAAATTCGGAAAGAAATGTAAAATCAGTGACCAGCTTGTAGAGCTTCCTATTGAGCATCAAATTTATGAATTAATAGATGCGGCAGGATCAGAGGGGTTGACCAGGAATGAGGTTATGGAGAGGCTTGGAATTGACAACAAGAAAAATTATACCCGCTTTGTTAGTATGTGCTCTAGATTTGATATGAGTCTGCAACCAGAAATGCACAAAAAAGCTGTGGCATATCGATTTCGGACATCTGGGAAACACAAGTCTGAATCAACTAATGCATTTCTTCGAAAATCAAAAGATGCCAATGACAGTAAAATTTTTAGTCTAAATGTTGGGAGTGTAGATGCTCTGAGATCAGATCAATTTCAAACTGGCTCAGTGTCAGATTGTTGGTCTCTGAACAGTGATACTGCTGGCCCTGAAAATATGAATAACATAGAGACTAATACTGACCCTTCTGCTGGATCTCTTGGGCGTAATGAGTTTGACGATATGCCTGAAACTTCCCAACAGTTACTTCTTGGACCAAAAGATACTACATCTGATTCTCAAGTTAGTTTAGTGAGCGCAGGAGTGGAACCAAATGGCGCCTTATCAGAAATACCAGCTGCTTTGTCAAAGCCACTTGCTAAAGGCTCAGATCCAAGGTACCCATGTATGTCTTTGACCGTGGATAACACTCGAAGGGAAAAGAGGATACTTGAACGGCTAGAGGGTGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGACAAGTGCACAGCAACTGACAGGAAAACAATTGACCGAATTTTACAAAAACTTCAACAGTTAGGGCACTGCAAGTGTATAGATATTAGCGTCCCTGTCGTCACAAATTTGGGCCGTACCCGAACCACTGTAGTGGTTCTGCACCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCTTGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAGTGGATCAGTTCCTGTACTAGAAGATGTTCAGAGAACTCAGACCCATGTTAGTGCACAGCGACAAACACTGAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTGCTGCATAGTTTCCTGTGGGATTACCTGTATGGTTCGTCCGGCTCTAATGATGCTTTATCATTTGGAAAAGATGTTATTGAGCCAAGAGATCCTCACGGTACTAGAAAATTGTTTTCTCTAGAAGCAACAATGAAGGCTATTCCAGTTGAGTTGTTTCTACAAGTTGCGGGATCCACCAAAAACTTTGAGGATATGATTGAGAAATGTAAGAGGGGTTTATGTCTCTCTGATCTCTCTCGTGAAGAATACAAGTCTCTGATGGATACCCATGCAACTGGAAGGCTCTCATTGGTGATTGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGATCACCACTCAGAAAATGGATTCCAAGTTCCCCCTCCCATGATTTCTGCATATGCATTGGAACTTAAGCCTTATGTAGAGGAACCTGTTTCAAAAGATGCGATATCTTTGAGTTTTGGATCACGTGATCTTCGTCCACGAATCAGACATGATTTTTCCCTAGCAAGCAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCGGACCCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAGCGTGATGAGCTCTTAAAGCGTGTTGTGAAGGATGATCCAAGTGAAAAGCTTTCATTCAAAGAGTGTGGAAAAATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACACAGATGAAGTTCAATCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATTATGAGGTAACTGGACAGCTGGAAGAGCAGATACATCCCACAGTATCTGATACTGTGGAACAATTAGAAGAACTAAATCTCTTGGTCACTTCTTATGAGCATGATTCACATTTGCAAGCATTGGACAATTGTCTAGAAACTGGGCAGGAACCTGAACCAAATGAAGATAATGAGGGGTGCCATTCAATTATTACCAAGGGTTCCTTTTCAAAGTTGAAGCCACTACGTCCTTCTAAAAGAAACAAGTTGCACTCAACACGTCAACGAAGGTTTTCATGGACAGAGGAGGCTGATAGGCAGTTGATTATCCAATATGTAAGACATCGTGCAACTCTTGGGGCAAAGATTCACCGGATAAATTGGGCTTCAGTTACTGACCTCCCAGCACCTCCTATTGCCTGCATGAAAAGAATGGCATCTCTGAAAAGTAACAGAAAGTTCAGATTAGCTGTAATGAGGCTTTGTAACATTCTCAGTGAACGATATGCAAAGATCTTGGACAAGACTCAGAACAGGTCACTGAACAAAGATGATTGCAATCTGCTTTTGCGAGATTCAATAGGTGAAGGTCATGACAGCAAACTTCCCAATATCAGTGATCATAATCCAGAGACAGGTCTTCAGGAAGAACCATGGGATGATTTTGATGACGTTTATGTTAAAAAGTCCCTTGAGGAGGTACTTCACCACAAGAGGTTGGCCAAATTTGATGCCTCCAAAAGAGTTGGATCTACCGGTGAGGACTGGTCGGATCTTAATACAAGTGAACGTGATCCCCAGGAATCTGAATTAATTGTATCAACTGCTCCATATGAAGATGTTCAGAATCATGGTAGCAGAGAAAAAATTTCGGCACGAAGATCTTGCTATCAACACCTTAATGAAAAATACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACACAAGTCTACAAATCATTAGCTGTTTCTAATGCTGTAGAGCTGTTTAAGCTTGTTTTCCTCAGTACCTCAACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCATTCTACGGCGGTACTCAGAGTGTGATCTATTCGCAGCTTTCAACTATCTTAGAGATAAAAAGTTCATGGTTGGAGGTAATGGCAGTCAAAAATTTTCTCTCTCACAACAGTTCTTGCATAATACTTCCGCATCACCATTTCCAATTAATTCTGGGAAGAGAGCTACTAAATTTGCCCATTGGCTCTACGAAAAAGATAAAGATCTTATGGAAGGGGGGATAGATCTCTCCACAGATTTACAGTGTGGGGACATATTCCATTTGTTTGCTCTAGTTTCTTCAGGTGAAATGTCAATTTCTCCATGCTTGCCAGATGAGGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCAGATATCAATGAACTTTTAGATGATGAAAAAACTAAAAAGCTGAAATCATTTGTGGCTGCAGAAGGTGAAATCATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCACGGTATCTGTAAGTCGTGCAGAGTTTTCTATAGCCAATTCTATAGATTTGTTCAAGGAAGACGCTCCTATTGGTGACAAACATTTTGGGGGAAATCATCAACTAGAATGTACATCTGATCAGAGTAGCTCATCACATTCTGACTGTATGAAAGAAATCTTCAATTCAGGCAGTACTGTCCCTGTATTGGAGATTGGTTGCGAGTCACCTTGGGAAGGTATGGTGGCTTATGCCGAACATTTGTTGCCATTACATCCTGCTCAAGATCAGTCAAGTCCCATCCAGCCTGAGGTCTTTAGGACTGTTTACATTGCTATTCAGAAAGCTGGCGATCAAGGTCTGAGCATAGAAGAAGTTTCCCGAATAATGAATATCCCAGGAGAAAAGATGACTGATCTTATCATTGATGTGCTTCAGACATTTGAACGAGTCTTAAAGGTCAATGCTTATGACTCTATTCGTGTTGTGGATTCTTTGTATCGTGGCAAGTACTTTATGACTTCAGTGTCTGGAAGTAATCAAAAACTCGAACCACCTTCCTGGAGAAAGCCACTAGGCGATGACGGCCATATACTCTTCCTTTCAGAAAATCGTGATAATGGTGCTGCTCCTTCAGAGAGGGAGATAAATGCTGATGTCCATAAATTGACAATTCTTAATTTTCCTGAAGAGGTTGACGAGCCCTCATATGTAAAACAAATTAGCAGTGTTCCTGAAAGCTATAGGGAAGGAAAAGGAGGAGATGCTGAGGATGAATCTTCTAGGTCCTCTAATGATAGACTATGCATGCCTATATTTCCATGGATAAATGGTGATGGGAGCACTAACAAGATTGTCTACAAGGGACTCAGGCGACGTGTGCTTGGGGTTGTGATGCAAAATCCAGCTATATTAGAGGATGAAATTATTCGCCGGATGGATGTATTGAATCCCCAGAGCTGTAGAAAATTGTTGGAATTGATGGTTTTGGATAACCATCTTCATGTGAGGAAGATGCATCAAACTGCGTGCATTGGGCCCCCACCAATTTTAGGTACCCTCCTTGGAAGCAGCTTCAAACCGTCAAAGCTGGTTTGTCGCGAGCACTATTTTGCAAATCCCATGAGTACATCCTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1885

Amino Acids

212.36

Weight (kDa)

6.6

Isoelectric Point (pI)

51.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 98 2.3e-33 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 109 - 192 1.2e-12 B-block binding subunit of TFIIIC
DUF7599 PF24538 232 - 314 6.8e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 333 - 417 1.1e-37 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 549 - 658 3.1e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 744 - 923 1.5e-82 Domain of unknown function (DUF7647)
DUF7645 PF24655 926 - 981 8.8e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2919
AarI CACCTGC 1 cut(s) 2890
AasI GACNNNNNNGTC 2 cut(s) 76, 5041
AatII GACGTC 1 cut(s) 2992
Acc36I ACCTGC 1 cut(s) 2890
Acc65I GGTACC 2 cut(s) 1629, 5570
AccB1I GGYRCC 4 cut(s) 1568, 1629, 3219, 5570
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 4 cut(s) 300, 2986, 3972, 5373
AccII CGCG 4 cut(s) 174, 351, 587, 5616
AccIII TCCGGA 1 cut(s) 270
AcoI YGGCCR 2 cut(s) 3741, 5101
AcuI CTGAAG 8 cut(s) 3393, 3647, 4332, 4422, 4934, 5022, 5133, 5223
AcvI CACGTG 1 cut(s) 2600
AcyI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
AflII CTTAAG 1 cut(s) 2540
AflIII ACRYGT 4 cut(s) 349, 955, 3299, 5392
AgeI ACCGGT 1 cut(s) 3776
AjiI CACGTC 2 cut(s) 3302, 5393
AjnI CCWGG 6 cut(s) 1091, 2725, 2892, 3819, 4912, 5075
AjuI GAANNNNNNNTTGG 2 cut(s) 1482, 1514
AleI CACNNNNGTG 1 cut(s) 1883
AloI GAACNNNNNNTCC 2 cut(s) 2487, 2519
Alw21I GWGCWC 8 cut(s) 690, 1163, 1763, 1985, 2065, 2808, 5360, 5622
Alw26I GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
Alw44I GTGCAC 2 cut(s) 1759, 2061
AlwNI CAGNNNCTG 5 cut(s) 1769, 2021, 3089, 3416, 5045
Ama87I CYCGRG 1 cut(s) 4528
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 8 cut(s) 55, 264, 286, 1396, 1864, 3741, 5101, 5552
ApaI GGGCCC 1 cut(s) 5556
ApaLI GTGCAC 2 cut(s) 1759, 2061
ArsI GACNNNNNNTTYG 4 cut(s) 339, 371, 4604, 4636
AseI ATTAAT 3 cut(s) 1061, 3833, 4215
AsiGI ACCGGT 1 cut(s) 3776
Asp700I GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
Asp718I GGTACC 2 cut(s) 1629, 5570
AspLEI GCGC 6 cut(s) 176, 481, 587, 589, 1550, 1571
AsuC2I CCSGG 2 cut(s) 4529, 4530
AsuII TTCGAA 2 cut(s) 1267, 2464
AvaI CYCGRG 1 cut(s) 4528
AvaII GGWCC 4 cut(s) 78, 1505, 2698, 5301
AxyI CCTNAGG 1 cut(s) 4824
BaeGI GKGCMC 4 cut(s) 1763, 1821, 2065, 5556
BaeI ACNNNNGTAYC 2 cut(s) 1621, 1654
BalI TGGCCA 1 cut(s) 3743
BamHI GGATCC 1 cut(s) 2309
BanI GGYRCC 4 cut(s) 1568, 1629, 3219, 5570
BanII GRGCYC 3 cut(s) 2808, 5220, 5556
BarI GAAGNNNNNNTAC 2 cut(s) 3057, 3089
BauI CACGAG 1 cut(s) 2376
BbrPI CACGTG 1 cut(s) 2600
BbsI GAAGAC 2 cut(s) 3653, 4602
Bbv12I GWGCWC 8 cut(s) 690, 1163, 1763, 1985, 2065, 2808, 5360, 5622
BbvCI CCTCAGC 1 cut(s) 5283
BceAI ACGGC 7 cut(s) 70, 311, 485, 1703, 1851, 4091, 5116
BcgI CGANNNNNNTGC 4 cut(s) 2888, 2922, 3888, 3922
BciT130I CCWGG 6 cut(s) 1093, 2727, 2894, 3821, 4914, 5077
BciVI GTATCC 2 cut(s) 1671, 2395
BclI TGATCA 3 cut(s) 2476, 3640, 4654
BcnI CCSGG 2 cut(s) 4529, 4530
BcoDI GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
BfmI CTRYAG 8 cut(s) 790, 1881, 3999, 4488, 4566, 4578, 5257, 5476
BfoI RGCGCY 1 cut(s) 1572
BfrI CTTAAG 1 cut(s) 2540
BfuAI ACCTGC 1 cut(s) 2890
BfuI GTATCC 2 cut(s) 1671, 2395
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 5 cut(s) 3005, 3534, 3902, 4270, 4291
BlpI GCTNAGC 2 cut(s) 1719, 2793
BmcAI AGTACT 2 cut(s) 4711, 5027
Bme1390I CCNGG 8 cut(s) 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
Bme18I GGWCC 4 cut(s) 78, 1505, 2698, 5301
BmeT110I CYCGRG 1 cut(s) 4528
BmgBI CACGTC 2 cut(s) 3302, 5393
BmrFI CCNGG 8 cut(s) 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
BmrI ACTGGG 1 cut(s) 3192
BmuI ACTGGG 1 cut(s) 3192
BoxI GACNNNNGTC 1 cut(s) 3171
BpiI GAAGAC 2 cut(s) 3653, 4602
BplI GAGNNNNNCTC 2 cut(s) 1900, 1932
BpmI CTGGAG 3 cut(s) 50, 2915, 5098
Bpu10I CCTNAGC 3 cut(s) 396, 624, 5283
Bpu1102I GCTNAGC 2 cut(s) 1719, 2793
Bpu14I TTCGAA 2 cut(s) 1267, 2464
BpuEI CTTGAG 4 cut(s) 107, 1515, 3734, 4782
BpuMI CCSGG 2 cut(s) 4529, 4530
Bsa29I ATCGAT 1 cut(s) 1216
BsaAI YACGTR 1 cut(s) 2600
BsaBI GATNNNNATC 5 cut(s) 602, 3579, 3645, 4269, 5511
BsaHI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BsaI GGTCTC 1 cut(s) 1380
BsaWI WCCGGW 3 cut(s) 270, 3393, 3776
BsaXI ACNNNNNCTCC 2 cut(s) 3942, 3972
Bse118I RCCGGY 2 cut(s) 706, 3776
Bse1I ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
Bse21I CCTNAGG 1 cut(s) 4824
Bse3DI GCAATG 2 cut(s) 717, 4845
Bse8I GATNNNNATC 5 cut(s) 602, 3579, 3645, 4269, 5511
BseAI TCCGGA 1 cut(s) 270
BseBI CCWGG 6 cut(s) 1093, 2727, 2894, 3821, 4914, 5077
BseCI ATCGAT 1 cut(s) 1216
BseJI GATNNNNATC 5 cut(s) 602, 3579, 3645, 4269, 5511
BseMI GCAATG 2 cut(s) 717, 4845
BseNI ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
BsePI GCGCGC 1 cut(s) 585
BseRI GAGGAG 6 cut(s) 79, 82, 85, 3341, 3731, 5289
BseSI GKGCMC 4 cut(s) 1763, 1821, 2065, 5556
BseYI CCCAGC 1 cut(s) 3424
BsgI GTGCAG 2 cut(s) 1877, 4575
Bsh1236I CGCG 4 cut(s) 174, 351, 587, 5616
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
BshNI GGYRCC 4 cut(s) 1568, 1629, 3219, 5570
BshTI ACCGGT 1 cut(s) 3776
BshVI ATCGAT 1 cut(s) 1216
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 8 cut(s) 690, 1163, 1763, 1985, 2065, 2808, 5360, 5622
BsiHKCI CYCGRG 1 cut(s) 4528
BsiSI CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
BsmAI GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
BsmBI CGTCTC 1 cut(s) 1742
BsmI GAATGC 1 cut(s) 4068
BsnI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
Bso31I GGTCTC 1 cut(s) 1380
BsoBI CYCGRG 1 cut(s) 4528
Bsp119I TTCGAA 2 cut(s) 1267, 2464
Bsp120I GGGCCC 1 cut(s) 5552
Bsp13I TCCGGA 1 cut(s) 270
Bsp1407I TGTACA 2 cut(s) 952, 4646
Bsp1720I GCTNAGC 2 cut(s) 1719, 2793
Bsp19I CCATGG 2 cut(s) 3674, 5336
Bsp68I TCGCGA 1 cut(s) 5616
BspANI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
BspDI ATCGAT 1 cut(s) 1216
BspEI TCCGGA 1 cut(s) 270
BspFNI CGCG 4 cut(s) 174, 351, 587, 5616
BspHI TCATGA 3 cut(s) 1939, 2740, 3613
BspMAI CTGCAG 1 cut(s) 4492
BspMI ACCTGC 1 cut(s) 2890
BspQI GCTCTTC 1 cut(s) 3060
BspT104I TTCGAA 2 cut(s) 1267, 2464
BspT107I GGYRCC 4 cut(s) 1568, 1629, 3219, 5570
BspTI CTTAAG 1 cut(s) 2540
BspTNI GGTCTC 1 cut(s) 1380
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 2 cut(s) 717, 4845
BsrFI RCCGGY 2 cut(s) 706, 3776
BsrGI TGTACA 2 cut(s) 952, 4646
BsrI ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
BssAI RCCGGY 2 cut(s) 706, 3776
BssHII GCGCGC 1 cut(s) 585
BssNI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BssSI CACGAG 1 cut(s) 2376
BssT1I CCWWGG 6 cut(s) 1625, 3237, 3674, 4745, 5336, 5578
Bst2BI CACGAG 1 cut(s) 2376
Bst2UI CCWGG 6 cut(s) 1093, 2727, 2894, 3821, 4914, 5077
Bst6I CTCTTC 7 cut(s) 211, 609, 2996, 3060, 4220, 5117, 5199
BstACI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BstAFI CTTAAG 1 cut(s) 2540
BstAUI TGTACA 2 cut(s) 952, 4646
BstBAI YACGTR 1 cut(s) 2600
BstBI TTCGAA 2 cut(s) 1267, 2464
BstDSI CCRYGG 3 cut(s) 1650, 3674, 5336
BstENI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
BstFNI CGCG 4 cut(s) 174, 351, 587, 5616
BstH2I RGCGCY 1 cut(s) 1572
BstHHI GCGC 6 cut(s) 176, 481, 587, 589, 1550, 1571
BstMAI GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 6 cut(s) 1093, 2727, 2894, 3821, 4914, 5077
BstNSI RCATGY 3 cut(s) 572, 959, 5326
BstPAI GACNNNNGTC 1 cut(s) 3171
BstSCI CCNGG 8 cut(s) 1091, 2725, 2892, 3819, 4527, 4528, 4912, 5075
BstSFI CTRYAG 8 cut(s) 790, 1881, 3999, 4488, 4566, 4578, 5257, 5476
BstSLI GKGCMC 4 cut(s) 1763, 1821, 2065, 5556
BstUI CGCG 4 cut(s) 174, 351, 587, 5616
BstV2I GAAGAC 2 cut(s) 3653, 4602
BstXI CCANNNNNNTGG 3 cut(s) 1885, 2414, 5076
Bsu15I ATCGAT 1 cut(s) 1216
Bsu36I CCTNAGG 1 cut(s) 4824
BsuI GTATCC 2 cut(s) 1671, 2395
BsuRI GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
BsuTUI ATCGAT 1 cut(s) 1216
BtgI CCRYGG 3 cut(s) 1650, 3674, 5336
BtgZI GCGATG 1 cut(s) 5109
BtrI CACGTC 2 cut(s) 3302, 5393
BtsI GCAGTG 2 cut(s) 1819, 5248
BtuMI TCGCGA 1 cut(s) 5616
BveI ACCTGC 1 cut(s) 2890
CaiI CAGNNNCTG 5 cut(s) 1769, 2021, 3089, 3416, 5045
CciI TCATGA 3 cut(s) 1939, 2740, 3613
CfoI GCGC 6 cut(s) 176, 481, 587, 589, 1550, 1571
Cfr10I RCCGGY 2 cut(s) 706, 3776
Cfr9I CCCGGG 1 cut(s) 4528
ClaI ATCGAT 1 cut(s) 1216
CseI GACGC 7 cut(s) 149, 236, 340, 878, 1831, 2918, 4607
CspAI ACCGGT 1 cut(s) 3776
CspCI CAANNNNNGTGG 2 cut(s) 2842, 2877
DinI GGCGCC 1 cut(s) 1570
DrdI GACNNNNNNGTC 2 cut(s) 76, 5041
DseDI GACNNNNNNGTC 2 cut(s) 76, 5041
EaeI YGGCCR 2 cut(s) 3741, 5101
Eam1104I CTCTTC 7 cut(s) 211, 609, 2996, 3060, 4220, 5117, 5199
EarI CTCTTC 7 cut(s) 211, 609, 2996, 3060, 4220, 5117, 5199
Ecl136II GAGCTC 1 cut(s) 2806
Eco130I CCWWGG 6 cut(s) 1625, 3237, 3674, 4745, 5336, 5578
Eco24I GRGCYC 3 cut(s) 2808, 5220, 5556
Eco31I GGTCTC 1 cut(s) 1380
Eco32I GATATC 2 cut(s) 2580, 4435
Eco47I GGWCC 4 cut(s) 78, 1505, 2698, 5301
Eco53kI GAGCTC 1 cut(s) 2806
Eco57I CTGAAG 8 cut(s) 3393, 3647, 4332, 4422, 4934, 5022, 5133, 5223
Eco72I CACGTG 1 cut(s) 2600
Eco81I CCTNAGG 1 cut(s) 4824
Eco88I CYCGRG 1 cut(s) 4528
EcoICRI GAGCTC 1 cut(s) 2806
EcoNI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
EcoO109I RGGNCCY 3 cut(s) 265, 5301, 5553
EcoRII CCWGG 6 cut(s) 1091, 2725, 2892, 3819, 4912, 5075
EcoRV GATATC 2 cut(s) 2580, 4435
EcoT14I CCWWGG 6 cut(s) 1625, 3237, 3674, 4745, 5336, 5578
EcoT22I ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
EcoT38I GRGCYC 3 cut(s) 2808, 5220, 5556
EgeI GGCGCC 1 cut(s) 1570
EheI GGCGCC 1 cut(s) 1570
ErhI CCWWGG 6 cut(s) 1625, 3237, 3674, 4745, 5336, 5578
Esp3I CGTCTC 1 cut(s) 1742
FalI AAGNNNNNCTT 4 cut(s) 447, 479, 3230, 3262
FauI CCCGC 3 cut(s) 1151, 1989, 2299
FauNDI CATATG 3 cut(s) 2527, 3853, 5224
FbaI TGATCA 3 cut(s) 2476, 3640, 4654
FblI GTMKAC 4 cut(s) 300, 2986, 3972, 5373
FriOI GRGCYC 3 cut(s) 2808, 5220, 5556
GlaI GCGC 6 cut(s) 175, 480, 586, 588, 1549, 1570
GsaI CCCAGC 1 cut(s) 3428
GsuI CTGGAG 3 cut(s) 50, 2915, 5098
HaeII RGCGCY 1 cut(s) 1572
HaeIII GGCC 8 cut(s) 57, 266, 288, 1398, 1866, 3743, 5103, 5554
HapII CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
HgaI GACGC 7 cut(s) 149, 236, 340, 878, 1831, 2918, 4607
HhaI GCGC 6 cut(s) 176, 481, 587, 589, 1550, 1571
Hin1I GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
Hin6I GCGC 6 cut(s) 174, 479, 585, 587, 1548, 1569
HinP1I GCGC 6 cut(s) 174, 479, 585, 587, 1548, 1569
HincII GTYRAC 5 cut(s) 301, 1089, 2933, 3305, 5212
HindII GTYRAC 5 cut(s) 301, 1089, 2933, 3305, 5212
HindIII AAGCTT 3 cut(s) 2843, 3938, 4013
HpaII CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
Hpy99I CGWCG 6 cut(s) 257, 356, 2451, 2994, 4515, 5394
HpyCH4IV ACGT 9 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 3811, 4423, 5392
HpySE526I ACGT 9 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 3811, 4423, 5392
Hsp92I GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
HspAI GCGC 6 cut(s) 174, 479, 585, 587, 1548, 1569
KasI GGCGCC 1 cut(s) 1568
Kpn2I TCCGGA 1 cut(s) 270
KpnI GGTACC 2 cut(s) 1633, 5574
Ksp22I TGATCA 3 cut(s) 2476, 3640, 4654
LguI GCTCTTC 1 cut(s) 3060
LmnI GCTCC 8 cut(s) 155, 400, 514, 2896, 3853, 4606, 5149, 5355
MaeII ACGT 9 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 3811, 4423, 5392
MbiI CCGCTC 1 cut(s) 150
MfeI CAATTG 2 cut(s) 1782, 3169
MlsI TGGCCA 1 cut(s) 3743
MluI ACGCGT 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 3743
Mly113I GGCGCC 1 cut(s) 1569
MlyI GAGTC 9 cut(s) 78, 197, 296, 1186, 3541, 4748, 4974, 4982, 5376
MmeI TCCRAC 5 cut(s) 123, 3748, 3771, 4120, 5469
Mox20I TGGCCA 1 cut(s) 3743
Mph1103I ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
MroI TCCGGA 1 cut(s) 270
MroXI GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
MscI TGGCCA 1 cut(s) 3743
MslI CAYNNNNRTG 8 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943, 5517
Msp20I TGGCCA 1 cut(s) 3743
MspA1I CMGCKG 3 cut(s) 1589, 2789, 3061
MspCI CTTAAG 1 cut(s) 2540
MspI CCGG 7 cut(s) 271, 707, 2178, 3394, 3777, 4529, 5449
MspR9I CCNGG 8 cut(s) 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
MunI CAATTG 2 cut(s) 1782, 3169
Mva1269I GAATGC 1 cut(s) 4068
MvaI CCWGG 6 cut(s) 1093, 2727, 2894, 3821, 4914, 5077
MvnI CGCG 4 cut(s) 174, 351, 587, 5616
NarI GGCGCC 1 cut(s) 1569
NciI CCSGG 2 cut(s) 4529, 4530
NcoI CCATGG 2 cut(s) 3674, 5336
NdeI CATATG 3 cut(s) 2527, 3853, 5224
NmeAIII GCCGAG 2 cut(s) 256, 543
NmuCI GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
NruI TCGCGA 1 cut(s) 5616
NsiI ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
NspI RCATGY 3 cut(s) 572, 959, 5326
NspV TTCGAA 2 cut(s) 1267, 2464
OliI CACNNNNGTG 1 cut(s) 1883
PaeI GCATGC 1 cut(s) 5326
PagI TCATGA 3 cut(s) 1939, 2740, 3613
PaqCI CACCTGC 1 cut(s) 2890
PauI GCGCGC 1 cut(s) 585
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 3060
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 351, 2605, 2989
PctI GAATGC 1 cut(s) 4068
PdmI GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
PflFI GACNNNGTC 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 5075
PinAI ACCGGT 1 cut(s) 3776
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 9 cut(s) 78, 197, 296, 1185, 3541, 4747, 4973, 4982, 5376
PluTI GGCGCC 1 cut(s) 1572
PmaCI CACGTG 1 cut(s) 2600
PmlI CACGTG 1 cut(s) 2600
PpsI GAGTC 9 cut(s) 78, 197, 296, 1185, 3541, 4747, 4973, 4982, 5376
Ppu21I YACGTR 1 cut(s) 2600
PpuMI RGGWCCY 1 cut(s) 5301
PscI ACATGT 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 3171
PshBI ATTAAT 3 cut(s) 1061, 3833, 4215
PsiI TTATAA 1 cut(s) 2919
Psp124BI GAGCTC 1 cut(s) 2808
Psp5II RGGWCCY 1 cut(s) 5301
Psp6I CCWGG 6 cut(s) 1091, 2725, 2892, 3819, 4912, 5075
PspCI CACGTG 1 cut(s) 2600
PspFI CCCAGC 1 cut(s) 3424
PspGI CCWGG 6 cut(s) 1091, 2725, 2892, 3819, 4912, 5075
PspOMI GGGCCC 1 cut(s) 5552
PspPPI RGGWCCY 1 cut(s) 5301
PstI CTGCAG 1 cut(s) 4492
PstNI CAGNNNCTG 5 cut(s) 1769, 2021, 3089, 3416, 5045
PsyI GACNNNGTC 1 cut(s) 305
PteI GCGCGC 1 cut(s) 585
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 3 cut(s) 1589, 2789, 3061
RruI TCGCGA 1 cut(s) 5616
RseI CAYNNNNRTG 8 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943, 5517
SacI GAGCTC 1 cut(s) 2808
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 3060
ScaI AGTACT 2 cut(s) 4711, 5027
SchI GAGTC 9 cut(s) 78, 197, 296, 1186, 3541, 4748, 4974, 4982, 5376
ScrFI CCNGG 8 cut(s) 1093, 2727, 2894, 3821, 4529, 4530, 4914, 5077
SfcI CTRYAG 8 cut(s) 790, 1881, 3999, 4488, 4566, 4578, 5257, 5476
SfoI GGCGCC 1 cut(s) 1570
SfuI TTCGAA 2 cut(s) 1267, 2464
SinI GGWCC 4 cut(s) 78, 1505, 2698, 5301
SmaI CCCGGG 1 cut(s) 4530
SmiMI CAYNNNNRTG 8 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943, 5517
SmlI CTYRAG 5 cut(s) 122, 1530, 2540, 3713, 4797
SmoI CTYRAG 5 cut(s) 122, 1530, 2540, 3713, 4797
SphI GCATGC 1 cut(s) 5326
SspDI GGCGCC 1 cut(s) 1568
SspI AATATT 1 cut(s) 826
SstI GAGCTC 1 cut(s) 2808
StyD4I CCNGG 8 cut(s) 1091, 2725, 2892, 3819, 4527, 4528, 4912, 5075
StyI CCWWGG 6 cut(s) 1625, 3237, 3674, 4745, 5336, 5578
TaiI ACGT 9 cut(s) 2602, 2986, 2992, 3271, 3304, 3696, 3814, 4426, 5395
TaqII GACCGA 1 cut(s) 1803
TatI WGTACW 8 cut(s) 555, 952, 2023, 3962, 4646, 4709, 5025, 5642
TauI GCSGC 2 cut(s) 1073, 2929
TseFI GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
Tsp45I GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
TspGWI ACGGA 3 cut(s) 142, 295, 1766
TspMI CCCGGG 1 cut(s) 4528
Tth111I GACNNNGTC 1 cut(s) 305
Vha464I CTTAAG 1 cut(s) 2540
VneI GTGCAC 2 cut(s) 1759, 2061
VpaK11BI GGWCC 4 cut(s) 78, 1505, 2698, 5301
VspI ATTAAT 3 cut(s) 1061, 3833, 4215
XagI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
XbaI TCTAGA 3 cut(s) 1163, 2257, 3175
XceI RCATGY 3 cut(s) 572, 959, 5326
XcmI CCANNNNNNNNNTGG 1 cut(s) 2489
XmaI CCCGGG 1 cut(s) 4528
XmiI GTMKAC 4 cut(s) 300, 2986, 3972, 5373
XmnI GAANNNNTTC 5 cut(s) 278, 2034, 3313, 3861, 5442
ZraI GACGTC 1 cut(s) 2990
ZrmI AGTACT 2 cut(s) 4711, 5027
Zsp2I ATGCAT 4 cut(s) 1261, 2533, 5324, 5535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.