Rh2CG430400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004044
Physical Location & Seq
Forward (+)
58542913 .. 58554395
11483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG430400.1

Sequence Viewer

Length: 4995 bp
ATGGACTCCATTCTAAACTCAGCGATCGAAGAAATCTGCTCGCACCTCCAAAACGGCCTCTCTCTCCAGACCCTCTGGTCCCGACTCTCCTCCTCCTCCAACCTCGACCTCTCTCCCACCCTCAAACAATCCCTATGGGACGCCCTCCGCTCCGTCCCCACTCTCAAATTCAGCGCCCAAAAAGACCGTTACGCCCCCACCGACCCTTCCATTCACTCCTTCCAAGACGCCGAGAACCTCAATTTGAAGCTCGTCGCCGATGAGGCCCTCCGGAACAACTTCATGGGCCTCTACAATGTCGACTCCGTCAATGCCAACCTGTCTGCCGTTCAACGCCACACTCTCGAACGCGTCGTCATGGCTAGGGGGAATGGGATTACACAAACTCAGCTTGCTAAGGAGCTTGGCATTGAGGGGAGAAACTTTCATTATGCTGTGAAGAACCTCGAATGTCAAGGGTTACTTGTACGGCAATCTGCGCTGTTAAGGACAAAAGAAGCTGGTGATGAGGGGGAGCCGAGGAACAATCCGAGTGTGACAACGACCAATATGTTGTACTTGTATCGACATGCAAAACATTTAAGCGCACAACAAAAGATTGAGATCACAAAGGAAGAGAGGGCTAAGGAGAGTTTTGTAAATGTAAAAGAAAGCCCTGCAAGTGGAGATGGTTTTCCTGGAAATGTGCTCGTAAAGGACTATCTACCGGCAATGAAAGCAGTCTGTAATAAACTTGAAGAAGCCAATGGCAAGGTTCTTGTCGTTTCGGATATTAAGAAGGAACTTGGCTACAGTAAAACATCAGGGCATAGAGCTTGGAGAAATATTTGTCAGAGGCTGAAAGCTGCTCATCTTGTGGAGGTGTTTGACGCTAAAGTGAATGGGAAGGTTGAGAGTTGCCTGCGTTTTGTAGAAAATTCTTCTCCAACGAAAGTTGAGCCAAGAGAAGTTGTACATGTAGATGAGGATTTTGTTGAAGAAGAACAAGTGAAATTCGGAAAGAAATGTAAAATCAGTGACCAGCTTGTAGAGCTTCCTATTGAGCATCAAATTTATGAATTAATAGATGCTGCAGGATCAGAGGGGTTGACCAGGAATGAGGTTATGGAGAGGCTTGGAATTGACAACAAGAAAAATTATACCCGCTTTGTTAGTATGTGCTCTAGATTTGATATGAGTCTGCAACCAGAAATGCACAAAAAAGCCGTGGCATATCGATTTCGGACATCTGGGAAACACAAGTCTGAATCAACTAATGCATTTCTTCGAAAATCAAAAGATGCCAATGACAGTAAAATTTTTAGTCTAAATGTTGGGAGTGTAGATGCTCTGAGATCAGATCAATTTCAAACTGGCTCAGTGTCAGATTGTTGGTCTCTGAACAGTGATACTGCTGGCCCTGAAAATATGAATAACATAGAGACTAATACTGACCCTTCTGCTGGATCTCTTGGGCGTAATGAGTTTGACGATATGCCTGAAACTTCCCAACAGTTACTTCTTGGACCAAAAGATACTACATCTGATTCTCAAGTTAGTTTAGTGAGCGCAGGAGTGGAACCAAATGGCGCCTTATCAGAAATACCAGCTGCTTTGTCAAAGCCACTTGCTAAAGGCTCAGATCCAAGGTATCCATGTATGTCTTTGACCGTGGATAACACTCGAAGGGAAAAGAGGATACTTGAACGGCTAGAGGGTGAGAAGTTTATTTTAAGAGCTGAGCTGTATAGATGGCTTGTGAGCTTAGAGACGGACAAGTGCACAGCAACTGACAGGAAAACAATTGACCGAATTTTACAAAAACTTCAACAGTTAGGGCACTGCAAGTGTATAGATATTAGCGTCCCTGTCGTCACAAATTTGGGCCGTACCCGAACCACTGTAGTGGTTCTGCATCCATCTGTTCAGAGTTTAACTCCTGAACTTGTTTCTGAAATTCATGATGCTTGGAGGTCTTTTGAGATTCAAAGTCGTGGCAAGTGCTCATCTCGGTGGCGGGAGAAGAACAGTGGATCAGTTCCTGTGCTAGAAGATGTTCAGAGAACTCAGACCCATGTTAGTGCACAGCGACAAACACTGAGTTCAGAAGCCATGCGTGCTAATGGATTTATTCTGGCAAAAATGGTTCGTGCAAAATTGCTGCATAGTTTCCTGTGGGATTACCTGTATGGTTCGTCCGGCTCTAATGATGCTTTATCATTTGGAAAAGATGTTATTGAGCCAAGAGATCCTCACGGTACTAGAAAATTGTTTTCTCTAGAAGCAACAATGAAGGCTATTCCAGTTGAGTTGTTTCTACAAGTTGCGGGATCCACCAAAAACTTTGAGGATATGATTGAGAAATGTAAGAGGGGTTTATGTCTCTCTGATCTCTCTCGTGAAGAATACAAGTCTCTGATGGATACCCATGCAACTGGAAGGCTCTCATTGGTGATCGACATTTTACGACGATTGAAGCTGATTCGAATGGTTTGTGATCACCACTCAGAAAATGGATTCCAAGTTCCCCCTCCCATGATTTCTGCATATGCATTGGAACTTAAGCCTTATGTAGAGGAACCTGTTTCAAAAGATGCGATATCTTTGAGTTTTGGATCACGTGATCTTCGTCCACGAATCAGACATGATTTTTCCCTATCAACCAGAGAAGCTGTTGATGAGTATTGGCAAACTTTAGAGTATTGTTATGCTGCTGCGGACCCAAGAGCTGCCTTACTTGCATTTCCTGGGTCTTGTGTTCATGAGGTGTCCCATCACAGGTCATGGACTAAACTTGGAGTTATGACAGCTGCTCAGCGTGATGAGCTCTTAAAGCGTGTTGTGAAGGATGATCCAAGTGAAAAGCTTTCATTCAAAGAGTGTGGAAAAATTGCGAAGGATCTCAATTTGACCCTGGAGCAGGTGCTTCGTGTGTATTATAATAAGCGGCGTCAACATCTTGATGGGTTGCAAAATAACACGGATGAAGTTCAATCTAAAAAACGTAGACGTCGTAAAAGGAAGAGATCTTCTGAATCTAGGTCAGTAGATTTTACTGAAAATGATGAGGTAACTGGACAGCTGGAAGAGCAGATACATCCCACAGTATCAGATACTGTGGAACAATTAGAAGAACTAAATCTCTTGGTCACTTCTTATGAGCATGATTCACATTTGCAAGCATTGGACAATTGTCTAGAAACTGGACAGGAACCTGAACCAAATGAAGATAATGAGGGGTGCCATTCAATTATTACTAAGGGTTCCTTTTCAAAGTTGAAGCCACTACGTCCTTCTAAAAGAAACAAGTTGCACTCAACACGTCAACGAAGGTTTTCATGGACAGAGGAGGCTGATAGGCAGTTGATTATCCAATATGTAAGACATCGTGCAACTCTTGGGGCAAAGATTCACCGGATAAATTGGGCTTCAGTTACTGACCTCCCAGCACCTCCTATTGCCTGCATGAAAAGAATGGCATCTCTGAAAAGTAACAGAAAGTTCAGATTAGCTGTAATGAGGCTTTGTAACATTCTCAGTGAACGATATGCAAAGATCTTGGAAAAGACTCAGAACAGGTCACTGAACAAAGATGATTGCAATCTGCTTTTGCGAGATTCAATAGGTGAAGGTCATGACAGCAAACTTCCCAATATCAGTGATCATAATCCAGAGACAGGTCTTCAGGAAGAACCATGGGATGATTTTGATGACGTTTATGTTAAAAAGTCCCTTGAGGAGGTACTTCACCACAAGAGGTTGGCCAAATTTGATGCCTCCAAAAGAGTTGGATCTACCGGTGAGGACTGGTCGGATCTTAATACAAGTGAATGTGATCCCCAGGAATCTGAATTAATTGTATCAACTGCTCCATATGAAGATGTTCAGAATCATGGTGGCAGAGAAAAAATTTCGGCACGAAGATCTTGCTATCAACACCTTAATGAAAAATACTTTAAGCTTTTGCATGGAGTTGATGTCAGTACACAAGTCTACAAATCATTAGCTGTTTCTAATGCTGTAGAGCTGTTTAAGCTTGTTTTCCTCAGTACCTCAACAGCACCAGAAGTGCCAAATCTGCTTGCAGGCATTCTACGGCGGTACTCAGAGTGTGATCTATTCGCAGCTTTCAACTATCTTAGAGATAAAAAGTTCATGGTTGGAGGTAATGGCAGTCAAAAATTTTCTCTCTCACAACAGTTCTTGCATAATACTTCCGCATCACCATTTCCAATTAATTCTGGGAAGAGAGCTACTAAATTCGCCCATTGGCTCTATGAAAAAGATAAAGATCTTATGGAAGGGGGGATCGATCTCTCCACAGATTTACAGTGTGGGGACATATTCCATTTGTTTGCTCTAGTTTCTTCAGGTGAACTGTCAATTTCTCCATGCTTGCCAGATGAAGGTGTGGGAGAGGCTGAAGAATCAAGAAGCTCAAAACGTAAAGCAGATATCAATGAACTTTTAGATGATGAAAAAACTAAAAAGCTGAAATCATTTGTGGCTGCAGAAGGTGAAATCATTTCCCGTCGAGAAAAAGGTTTCCCGGGTATCACAGTATCTGTAAGTCGTGCAGAGTTTTCTATAGCCAATTCTATAGATTTGTTCAAGGAAGACGCTCCTATTGGTGACAAACATTTTGGGGGAAATCATCAACTAGAATGTACATCTGATCAGAGTAGCTTATCACATTCTGACTGTATGAAAGAAATCTTCAATTCAGGCAGTAATGTCCCTGTATTAGAGATTGGTTGCGAGTCACCTTGGGAAGGTATGGTGGCTTATGCCGAACATTTGTTGCCATTACATCCTGCTCAAGATCAGTCAAGTCCCATCCAGCCTGAGGTCTTTAGGACTGTTTACATTGCTATTCAGAAAGCTGGTGATCAAGGTCTGAGCATAGAAGAAGTTTCCCGAATAATGAATATCCCAGGAGAAAAGATGACTGATCTTATCATTGATGTGCTTCGGACATTTGAACGAGTCTTAAAGGTTAGTTTTCTGATGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B-block_TFIIIC

Protein Analysis

1664

Amino Acids

187.37

Weight (kDa)

6.47

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_GTF3C1_N PF23704 1 - 98 2.2e-34 General transcription factor 3C polypeptide 1, winged-helix domain
B-block_TFIIIC PF04182 109 - 192 1e-12 B-block binding subunit of TFIIIC
DUF7599 PF24538 232 - 314 6.3e-36 Domain of unknown function (DUF7599)
DUF7646 PF24657 333 - 417 9.7e-38 Domain of unknown function (DUF7646)
WHD_GTF3C1 PF24101 549 - 658 2.7e-40 GTF3C1, extended winged-helix domain
DUF7647 PF24658 744 - 923 7.2e-83 Domain of unknown function (DUF7647)
DUF7645 PF24655 926 - 981 7.6e-30 Domain of unknown function (DUF7645)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2919
AarI CACCTGC 1 cut(s) 2890
AasI GACNNNNNNGTC 1 cut(s) 76
AatII GACGTC 1 cut(s) 2992
Acc36I ACCTGC 1 cut(s) 2890
AccB1I GGYRCC 2 cut(s) 1568, 3219
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 3 cut(s) 300, 2986, 3972
AccII CGCG 1 cut(s) 351
AccIII TCCGGA 1 cut(s) 270
AciI CCGC 8 cut(s) 148, 1144, 1996, 2306, 2696, 2926, 4078, 4197
AcoI YGGCCR 1 cut(s) 3741
AcuI CTGAAG 4 cut(s) 3393, 3647, 4332, 4422
AcvI CACGTG 1 cut(s) 2600
AcyI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
AfiI CCNNNNNNNGG 9 cut(s) 662, 1442, 2757, 2899, 3656, 3718, 4385, 4751, 4825
AflII CTTAAG 1 cut(s) 2540
AflIII ACRYGT 3 cut(s) 349, 955, 3299
AgeI ACCGGT 1 cut(s) 3776
AjiI CACGTC 1 cut(s) 3302
AjnI CCWGG 6 cut(s) 676, 1091, 2725, 2892, 3819, 4912
AjuI GAANNNNNNNTTGG 2 cut(s) 1482, 1514
AleI CACNNNNGTG 1 cut(s) 1883
AloI GAACNNNNNNTCC 2 cut(s) 2487, 2519
Alw21I GWGCWC 6 cut(s) 690, 1163, 1763, 1985, 2065, 2808
Alw26I GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
Alw44I GTGCAC 2 cut(s) 1759, 2061
AlwNI CAGNNNCTG 6 cut(s) 838, 1769, 2021, 3095, 3416, 4544
Ama87I CYCGRG 1 cut(s) 4528
Aor13HI TCCGGA 1 cut(s) 270
AoxI GGCC 6 cut(s) 55, 264, 286, 1396, 1864, 3741
ApaLI GTGCAC 2 cut(s) 1759, 2061
ArsI GACNNNNNNTTYG 4 cut(s) 339, 371, 4604, 4636
AseI ATTAAT 3 cut(s) 1061, 3833, 4215
AsiGI ACCGGT 1 cut(s) 3776
Asp700I GAANNNNTTC 4 cut(s) 278, 2034, 3313, 3861
AspLEI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
AspS9I GGNCC 7 cut(s) 78, 265, 286, 1397, 1505, 1864, 2698
AsuC2I CCSGG 2 cut(s) 4529, 4530
AsuII TTCGAA 2 cut(s) 1267, 2464
AvaI CYCGRG 1 cut(s) 4528
AvaII GGWCC 3 cut(s) 78, 1505, 2698
AxyI CCTNAGG 1 cut(s) 4824
BaeGI GKGCMC 3 cut(s) 1763, 1821, 2065
BalI TGGCCA 1 cut(s) 3743
BamHI GGATCC 1 cut(s) 2309
BanI GGYRCC 2 cut(s) 1568, 3219
BanII GRGCYC 1 cut(s) 2808
BarI GAAGNNNNNNTAC 2 cut(s) 3057, 3089
BauI CACGAG 1 cut(s) 2376
BbrPI CACGTG 1 cut(s) 2600
BbsI GAAGAC 2 cut(s) 3653, 4602
Bbv12I GWGCWC 6 cut(s) 690, 1163, 1763, 1985, 2065, 2808
BccI CCATC 7 cut(s) 662, 1725, 1906, 2392, 2760, 2936, 4823
BceAI ACGGC 7 cut(s) 70, 311, 485, 1190, 1703, 1851, 4091
BcgI CGANNNNNNTGC 4 cut(s) 2888, 2922, 3888, 3922
BciT130I CCWGG 6 cut(s) 678, 1093, 2727, 2894, 3821, 4914
BciVI GTATCC 3 cut(s) 1641, 1671, 2395
BclI TGATCA 4 cut(s) 2476, 3640, 4654, 4867
BcnI CCSGG 2 cut(s) 4529, 4530
BcoDI GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
BfmI CTRYAG 7 cut(s) 790, 1071, 1881, 3999, 4488, 4566, 4578
BfoI RGCGCY 2 cut(s) 177, 1572
BfrI CTTAAG 1 cut(s) 2540
BfuAI ACCTGC 1 cut(s) 2890
BfuI GTATCC 3 cut(s) 1641, 1671, 2395
BglI GCCNNNNNGGC 1 cut(s) 263
BglII AGATCT 4 cut(s) 3005, 3534, 3902, 4270
BlpI GCTNAGC 2 cut(s) 1719, 2793
Bme1390I CCNGG 8 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914
Bme18I GGWCC 3 cut(s) 78, 1505, 2698
BmeT110I CYCGRG 1 cut(s) 4528
BmgBI CACGTC 1 cut(s) 3302
BmgT120I GGNCC 7 cut(s) 78, 265, 286, 1397, 1505, 1864, 2698
BmrFI CCNGG 8 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914
BoxI GACNNNNGTC 1 cut(s) 3171
BpiI GAAGAC 2 cut(s) 3653, 4602
BplI GAGNNNNNCTC 2 cut(s) 1900, 1932
BpmI CTGGAG 2 cut(s) 50, 2915
Bpu10I CCTNAGC 2 cut(s) 396, 624
Bpu1102I GCTNAGC 2 cut(s) 1719, 2793
Bpu14I TTCGAA 2 cut(s) 1267, 2464
BpuEI CTTGAG 3 cut(s) 1515, 3734, 4782
BpuMI CCSGG 2 cut(s) 4529, 4530
Bsa29I ATCGAT 2 cut(s) 1216, 4290
BsaAI YACGTR 1 cut(s) 2600
BsaBI GATNNNNATC 4 cut(s) 602, 3579, 3645, 4269
BsaHI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BsaI GGTCTC 1 cut(s) 1380
BsaWI WCCGGW 3 cut(s) 270, 3393, 3776
BsaXI ACNNNNNCTCC 2 cut(s) 3942, 3972
Bsc4I CCNNNNNNNGG 9 cut(s) 662, 1442, 2757, 2899, 3656, 3718, 4385, 4751, 4825
Bse118I RCCGGY 2 cut(s) 706, 3776
Bse1I ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
Bse21I CCTNAGG 1 cut(s) 4824
Bse3DI GCAATG 2 cut(s) 717, 4845
Bse8I GATNNNNATC 4 cut(s) 602, 3579, 3645, 4269
BseAI TCCGGA 1 cut(s) 270
BseBI CCWGG 6 cut(s) 678, 1093, 2727, 2894, 3821, 4914
BseCI ATCGAT 2 cut(s) 1216, 4290
BseGI GGATG 7 cut(s) 1894, 2833, 2968, 3076, 3685, 4789, 4815
BseJI GATNNNNATC 4 cut(s) 602, 3579, 3645, 4269
BseLI CCNNNNNNNGG 9 cut(s) 662, 1442, 2757, 2899, 3656, 3718, 4385, 4751, 4825
BseMI GCAATG 2 cut(s) 717, 4845
BseNI ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
BseRI GAGGAG 5 cut(s) 79, 82, 85, 3341, 3731
BseSI GKGCMC 3 cut(s) 1763, 1821, 2065
BseYI CCCAGC 1 cut(s) 3424
BsgI GTGCAG 1 cut(s) 4575
Bsh1236I CGCG 1 cut(s) 351
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 6 cut(s) 57, 266, 288, 1398, 1866, 3743
BshNI GGYRCC 2 cut(s) 1568, 3219
BshTI ACCGGT 1 cut(s) 3776
BshVI ATCGAT 2 cut(s) 1216, 4290
BsiEI CGRYCG 1 cut(s) 27
BsiHKAI GWGCWC 6 cut(s) 690, 1163, 1763, 1985, 2065, 2808
BsiHKCI CYCGRG 1 cut(s) 4528
BsiSI CCGG 6 cut(s) 271, 707, 2178, 3394, 3777, 4529
BslFI GGGAC 9 cut(s) 64, 140, 152, 1829, 2734, 3694, 4331, 4700, 4797
BslI CCNNNNNNNGG 9 cut(s) 662, 1442, 2757, 2899, 3656, 3718, 4385, 4751, 4825
BsmAI GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
BsmBI CGTCTC 1 cut(s) 1742
BsmFI GGGAC 9 cut(s) 64, 140, 152, 1829, 2734, 3694, 4331, 4700, 4797
BsmI GAATGC 1 cut(s) 4068
BsnI GGCC 6 cut(s) 57, 266, 288, 1398, 1866, 3743
Bso31I GGTCTC 1 cut(s) 1380
BsoBI CYCGRG 1 cut(s) 4528
Bsp119I TTCGAA 2 cut(s) 1267, 2464
Bsp1286I GDGCHC 7 cut(s) 690, 1163, 1763, 1821, 1985, 2065, 2808
Bsp13I TCCGGA 1 cut(s) 270
Bsp1407I TGTACA 2 cut(s) 952, 4646
Bsp1720I GCTNAGC 2 cut(s) 1719, 2793
Bsp19I CCATGG 1 cut(s) 3674
BspACI CCGC 8 cut(s) 148, 1144, 1996, 2306, 2696, 2926, 4078, 4197
BspANI GGCC 6 cut(s) 57, 266, 288, 1398, 1866, 3743
BspDI ATCGAT 2 cut(s) 1216, 4290
BspEI TCCGGA 1 cut(s) 270
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 3 cut(s) 1939, 2740, 3613
BspMAI CTGCAG 2 cut(s) 1075, 4492
BspMI ACCTGC 1 cut(s) 2890
BspQI GCTCTTC 1 cut(s) 3060
BspT104I TTCGAA 2 cut(s) 1267, 2464
BspT107I GGYRCC 2 cut(s) 1568, 3219
BspTI CTTAAG 1 cut(s) 2540
BspTNI GGTCTC 1 cut(s) 1380
BsrBI CCGCTC 1 cut(s) 150
BsrDI GCAATG 2 cut(s) 717, 4845
BsrFI RCCGGY 2 cut(s) 706, 3776
BsrGI TGTACA 2 cut(s) 952, 4646
BsrI ACTGG 6 cut(s) 1357, 2282, 2419, 3058, 3187, 3791
BssAI RCCGGY 2 cut(s) 706, 3776
BssNI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BssSI CACGAG 1 cut(s) 2376
BssT1I CCWWGG 3 cut(s) 1625, 3674, 4745
Bst2BI CACGAG 1 cut(s) 2376
Bst2UI CCWGG 6 cut(s) 678, 1093, 2727, 2894, 3821, 4914
Bst6I CTCTTC 4 cut(s) 609, 2996, 3060, 4220
BstACI GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
BstAFI CTTAAG 1 cut(s) 2540
BstAUI TGTACA 2 cut(s) 952, 4646
BstBAI YACGTR 1 cut(s) 2600
BstBI TTCGAA 2 cut(s) 1267, 2464
BstDSI CCRYGG 3 cut(s) 1206, 1650, 3674
BstENI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
BstF5I GGATG 7 cut(s) 1894, 2833, 2968, 3076, 3685, 4789, 4815
BstFNI CGCG 1 cut(s) 351
BstH2I RGCGCY 2 cut(s) 177, 1572
BstHHI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
BstMAI GTCTC 6 cut(s) 1380, 1415, 1742, 2366, 2397, 3647
BstMCI CGRYCG 1 cut(s) 27
BstNI CCWGG 6 cut(s) 678, 1093, 2727, 2894, 3821, 4914
BstNSI RCATGY 2 cut(s) 572, 959
BstPAI GACNNNNGTC 1 cut(s) 3171
BstSCI CCNGG 8 cut(s) 676, 1091, 2725, 2892, 3819, 4527, 4528, 4912
BstSFI CTRYAG 7 cut(s) 790, 1071, 1881, 3999, 4488, 4566, 4578
BstSLI GKGCMC 3 cut(s) 1763, 1821, 2065
BstUI CGCG 1 cut(s) 351
BstV2I GAAGAC 2 cut(s) 3653, 4602
BstXI CCANNNNNNTGG 2 cut(s) 1885, 2414
Bsu15I ATCGAT 2 cut(s) 1216, 4290
Bsu36I CCTNAGG 1 cut(s) 4824
BsuI GTATCC 3 cut(s) 1641, 1671, 2395
BsuRI GGCC 6 cut(s) 57, 266, 288, 1398, 1866, 3743
BsuTUI ATCGAT 2 cut(s) 1216, 4290
BtgI CCRYGG 3 cut(s) 1206, 1650, 3674
BtrI CACGTC 1 cut(s) 3302
BtsCI GGATG 7 cut(s) 1894, 2833, 2968, 3076, 3685, 4789, 4815
BtsI GCAGTG 1 cut(s) 1819
BveI ACCTGC 1 cut(s) 2890
CaiI CAGNNNCTG 6 cut(s) 838, 1769, 2021, 3095, 3416, 4544
CciI TCATGA 3 cut(s) 1939, 2740, 3613
CfoI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
Cfr10I RCCGGY 2 cut(s) 706, 3776
Cfr13I GGNCC 7 cut(s) 78, 265, 286, 1397, 1505, 1864, 2698
Cfr9I CCCGGG 1 cut(s) 4528
ClaI ATCGAT 2 cut(s) 1216, 4290
CseI GACGC 7 cut(s) 149, 236, 340, 878, 1831, 2918, 4607
CspAI ACCGGT 1 cut(s) 3776
CspCI CAANNNNNGTGG 2 cut(s) 2842, 2877
DinI GGCGCC 1 cut(s) 1570
DrdI GACNNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 3741
Eam1104I CTCTTC 4 cut(s) 609, 2996, 3060, 4220
EarI CTCTTC 4 cut(s) 609, 2996, 3060, 4220
Ecl136II GAGCTC 1 cut(s) 2806
Eco130I CCWWGG 3 cut(s) 1625, 3674, 4745
Eco24I GRGCYC 1 cut(s) 2808
Eco31I GGTCTC 1 cut(s) 1380
Eco32I GATATC 2 cut(s) 2580, 4435
Eco47I GGWCC 3 cut(s) 78, 1505, 2698
Eco53kI GAGCTC 1 cut(s) 2806
Eco57I CTGAAG 4 cut(s) 3393, 3647, 4332, 4422
Eco72I CACGTG 1 cut(s) 2600
Eco81I CCTNAGG 1 cut(s) 4824
Eco88I CYCGRG 1 cut(s) 4528
EcoICRI GAGCTC 1 cut(s) 2806
EcoNI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
EcoO109I RGGNCCY 1 cut(s) 265
EcoRII CCWGG 6 cut(s) 676, 1091, 2725, 2892, 3819, 4912
EcoRV GATATC 2 cut(s) 2580, 4435
EcoT14I CCWWGG 3 cut(s) 1625, 3674, 4745
EcoT22I ATGCAT 2 cut(s) 1261, 2533
EcoT38I GRGCYC 1 cut(s) 2808
EgeI GGCGCC 1 cut(s) 1570
EheI GGCGCC 1 cut(s) 1570
ErhI CCWWGG 3 cut(s) 1625, 3674, 4745
Esp3I CGTCTC 1 cut(s) 1742
FalI AAGNNNNNCTT 4 cut(s) 447, 479, 3230, 3262
FaqI GGGAC 9 cut(s) 64, 140, 152, 1829, 2734, 3694, 4331, 4700, 4797
FauI CCCGC 3 cut(s) 1151, 1989, 2299
FauNDI CATATG 2 cut(s) 2527, 3853
FbaI TGATCA 4 cut(s) 2476, 3640, 4654, 4867
FblI GTMKAC 3 cut(s) 300, 2986, 3972
FokI GGATG 7 cut(s) 1881, 2840, 2975, 3063, 3692, 4776, 4802
FriOI GRGCYC 1 cut(s) 2808
GlaI GCGC 5 cut(s) 175, 480, 586, 1549, 1570
GsaI CCCAGC 1 cut(s) 3428
GsuI CTGGAG 2 cut(s) 50, 2915
HaeII RGCGCY 2 cut(s) 177, 1572
HaeIII GGCC 6 cut(s) 57, 266, 288, 1398, 1866, 3743
HapII CCGG 6 cut(s) 271, 707, 2178, 3394, 3777, 4529
HgaI GACGC 7 cut(s) 149, 236, 340, 878, 1831, 2918, 4607
HhaI GCGC 5 cut(s) 176, 481, 587, 1550, 1571
Hin1I GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
Hin6I GCGC 5 cut(s) 174, 479, 585, 1548, 1569
HinP1I GCGC 5 cut(s) 174, 479, 585, 1548, 1569
HincII GTYRAC 4 cut(s) 301, 1089, 2933, 3305
HindII GTYRAC 4 cut(s) 301, 1089, 2933, 3305
HindIII AAGCTT 3 cut(s) 2843, 3938, 4013
HpaII CCGG 6 cut(s) 271, 707, 2178, 3394, 3777, 4529
Hpy99I CGWCG 5 cut(s) 257, 356, 2451, 2994, 4515
HpyCH4IV ACGT 7 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 4423
HpySE526I ACGT 7 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 4423
Hsp92I GRCGYC 5 cut(s) 141, 228, 1569, 2929, 2989
HspAI GCGC 5 cut(s) 174, 479, 585, 1548, 1569
KasI GGCGCC 1 cut(s) 1568
Kpn2I TCCGGA 1 cut(s) 270
Ksp22I TGATCA 4 cut(s) 2476, 3640, 4654, 4867
LguI GCTCTTC 1 cut(s) 3060
LmnI GCTCC 6 cut(s) 155, 400, 514, 2896, 3853, 4606
MaeII ACGT 7 cut(s) 2599, 2983, 2989, 3268, 3301, 3693, 4423
MbiI CCGCTC 1 cut(s) 150
MfeI CAATTG 2 cut(s) 1782, 3169
MhlI GDGCHC 7 cut(s) 690, 1163, 1763, 1821, 1985, 2065, 2808
MlsI TGGCCA 1 cut(s) 3743
MluI ACGCGT 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 3743
Mly113I GGCGCC 1 cut(s) 1569
MlyI GAGTC 6 cut(s) 78, 296, 1186, 3541, 4748, 4974
MmeI TCCRAC 5 cut(s) 123, 950, 3748, 3771, 4120
Mox20I TGGCCA 1 cut(s) 3743
Mph1103I ATGCAT 2 cut(s) 1261, 2533
MroI TCCGGA 1 cut(s) 270
MroXI GAANNNNTTC 4 cut(s) 278, 2034, 3313, 3861
MscI TGGCCA 1 cut(s) 3743
MslI CAYNNNNRTG 7 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943
Msp20I TGGCCA 1 cut(s) 3743
MspA1I CMGCKG 3 cut(s) 1589, 2789, 3061
MspCI CTTAAG 1 cut(s) 2540
MspI CCGG 6 cut(s) 271, 707, 2178, 3394, 3777, 4529
MspR9I CCNGG 8 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914
MunI CAATTG 2 cut(s) 1782, 3169
Mva1269I GAATGC 1 cut(s) 4068
MvaI CCWGG 6 cut(s) 678, 1093, 2727, 2894, 3821, 4914
MvnI CGCG 1 cut(s) 351
NarI GGCGCC 1 cut(s) 1569
NciI CCSGG 2 cut(s) 4529, 4530
NcoI CCATGG 1 cut(s) 3674
NdeI CATATG 2 cut(s) 2527, 3853
NmeAIII GCCGAG 2 cut(s) 256, 543
NmuCI GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
NsiI ATGCAT 2 cut(s) 1261, 2533
NspI RCATGY 2 cut(s) 572, 959
NspV TTCGAA 2 cut(s) 1267, 2464
OliI CACNNNNGTG 1 cut(s) 1883
PagI TCATGA 3 cut(s) 1939, 2740, 3613
PaqCI CACCTGC 1 cut(s) 2890
PciI ACATGT 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 3060
PcsI WCGNNNNNNNCGW 4 cut(s) 198, 351, 2605, 2989
PctI GAATGC 1 cut(s) 4068
PdmI GAANNNNTTC 4 cut(s) 278, 2034, 3313, 3861
PflFI GACNNNGTC 1 cut(s) 305
PfoI TCCNGGA 1 cut(s) 676
PinAI ACCGGT 1 cut(s) 3776
Ple19I CGATCG 1 cut(s) 27
PleI GAGTC 6 cut(s) 78, 296, 1185, 3541, 4747, 4973
PluTI GGCGCC 1 cut(s) 1572
PmaCI CACGTG 1 cut(s) 2600
PmlI CACGTG 1 cut(s) 2600
PpsI GAGTC 6 cut(s) 78, 296, 1185, 3541, 4747, 4973
Ppu21I YACGTR 1 cut(s) 2600
PscI ACATGT 1 cut(s) 955
PshAI GACNNNNGTC 1 cut(s) 3171
PshBI ATTAAT 3 cut(s) 1061, 3833, 4215
PsiI TTATAA 1 cut(s) 2919
Psp124BI GAGCTC 1 cut(s) 2808
Psp6I CCWGG 6 cut(s) 676, 1091, 2725, 2892, 3819, 4912
PspCI CACGTG 1 cut(s) 2600
PspFI CCCAGC 1 cut(s) 3424
PspGI CCWGG 6 cut(s) 676, 1091, 2725, 2892, 3819, 4912
PspPI GGNCC 7 cut(s) 78, 265, 286, 1397, 1505, 1864, 2698
PstI CTGCAG 2 cut(s) 1075, 4492
PstNI CAGNNNCTG 6 cut(s) 838, 1769, 2021, 3095, 3416, 4544
PsyI GACNNNGTC 1 cut(s) 305
PvuI CGATCG 1 cut(s) 27
PvuII CAGCTG 3 cut(s) 1589, 2789, 3061
RseI CAYNNNNRTG 7 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943
SacI GAGCTC 1 cut(s) 2808
SalI GTCGAC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 3060
Sau96I GGNCC 7 cut(s) 78, 265, 286, 1397, 1505, 1864, 2698
SchI GAGTC 6 cut(s) 78, 296, 1186, 3541, 4748, 4974
ScrFI CCNGG 8 cut(s) 678, 1093, 2727, 2894, 3821, 4529, 4530, 4914
SduI GDGCHC 7 cut(s) 690, 1163, 1763, 1821, 1985, 2065, 2808
SfcI CTRYAG 7 cut(s) 790, 1071, 1881, 3999, 4488, 4566, 4578
SfoI GGCGCC 1 cut(s) 1570
SfuI TTCGAA 2 cut(s) 1267, 2464
SinI GGWCC 3 cut(s) 78, 1505, 2698
SmaI CCCGGG 1 cut(s) 4530
SmiMI CAYNNNNRTG 7 cut(s) 960, 1883, 1990, 2058, 2940, 3921, 4943
SmlI CTYRAG 4 cut(s) 1530, 2540, 3713, 4797
SmoI CTYRAG 4 cut(s) 1530, 2540, 3713, 4797
SsiI CCGC 8 cut(s) 148, 1144, 1996, 2306, 2696, 2926, 4078, 4197
SspDI GGCGCC 1 cut(s) 1568
SspI AATATT 1 cut(s) 826
SstI GAGCTC 1 cut(s) 2808
StyD4I CCNGG 8 cut(s) 676, 1091, 2725, 2892, 3819, 4527, 4528, 4912
StyI CCWWGG 3 cut(s) 1625, 3674, 4745
TaiI ACGT 7 cut(s) 2602, 2986, 2992, 3271, 3304, 3696, 4426
TaqII GACCGA 1 cut(s) 1803
TatI WGTACW 4 cut(s) 555, 952, 3962, 4646
TauI GCSGC 1 cut(s) 2929
TseFI GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
Tsp45I GTSAC 7 cut(s) 535, 1016, 1852, 3127, 3558, 4610, 4740
TspGWI ACGGA 4 cut(s) 142, 295, 1766, 2975
TspMI CCCGGG 1 cut(s) 4528
Tth111I GACNNNGTC 1 cut(s) 305
Vha464I CTTAAG 1 cut(s) 2540
VneI GTGCAC 2 cut(s) 1759, 2061
VpaK11BI GGWCC 3 cut(s) 78, 1505, 2698
VspI ATTAAT 3 cut(s) 1061, 3833, 4215
XagI CCTNNNNNAGG 3 cut(s) 2897, 3716, 4749
XbaI TCTAGA 3 cut(s) 1163, 2257, 3175
XceI RCATGY 2 cut(s) 572, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 2489
XmaI CCCGGG 1 cut(s) 4528
XmiI GTMKAC 3 cut(s) 300, 2986, 3972
XmnI GAANNNNTTC 4 cut(s) 278, 2034, 3313, 3861
ZraI GACGTC 1 cut(s) 2990
Zsp2I ATGCAT 2 cut(s) 1261, 2533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.