FvH4_6g40060

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006417
Physical Location & Seq
Reverse (-)
31638495 .. 31640291
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g40060.t1

Sequence Viewer

Length: 1368 bp
ATGGAGAAAATTGAGGTCAGTATAATTTCCAAACACACCATCAAACCATCAGCTTCCTCTACACCACTTCAGCCTTACAACCTTACCCTCCTGGACCAGCTCACTCCTCCAGCGTATGTCCCCATGGTATTCTTCTACCCCATTACTGACCATGTCTTCAATCTTCCTCAAACCCTAGCTGACTTAAGACAAGCCCTTTCCGAGACTCTCACTTTGTACTATCCACTCTCTGGAAGGGTCAAAAACAACCTATACATCGATGATTTTGAAGAGGGTGTCCCTTACCTTGAGGCTCGAGTGAATTGTGACATGACTGATTTTCTAAGGCTTCGGAAAATCGAGTGCCTAAATGAGTTTGTTCCAATAAAACCATTTAGTATGGAAGCAATATCTGATGAGCGTTACCCCTTGCTTGGAGTTCAAGTCAACATTTTGGACTCCGGAATAGCAATCTGTGTCTTCATCTCTCACAAGCTCATCGATGGAAGAACGGCAGACTGTTTTCTCAAGTCCTGGGGTGCTATTTTTCGAGGGTGTCGTGAAGATATCATACATCCTAGTCTCTCTGAAGCAGCATTGCTTTTCCCACCGAGAGATGACTTGCCTGAAAAATATGCCGGTCAGATGGAAAGGGTATGGTTTGCCGGAAAAAAAGTTGCTACAAGGAGATTTGTATTTGGTGCAAAAGCCATATCTTCAATTCAAGATGAAGCGAAGAGCGAGCCAGTGCCCAAGCCATCACGAGTTCAGGCCGTCACTGGTTTTCTCTGGAAACATCTAATCGCTGCTTCTCGGGCCCTAACATTAGGTACTACTTCAACAAGACTTTCTATAGCAGCCCAGGCAGTGAACTTAAGAACACGGATTAACATGGAGACAGTGTTGGATAATGCCATTGGAAACTTGATGTGGTGGGCACAGGCCATACTAGAGCTATGTCATACAACACCAGAGATTAGTGATCTTAAGCTGTGTGACTTGGTTAACTTGCTCAATGGATCTGTCAAACAATGTAACAGTGATTACTTTGAGACTTTCAAGGGTAAAGAGGGATATGGAAGAATGTGTGAGTATCTAGATTTTCAGAGGACTATGAGTTCTATGGAACCATCACGAGAGATTTATTTATTCTCGAGCTGGACTAATTTTTTCAACGCACTTGATTTTGGATGGGGGAGGACATCATGGATTGGAGTTGCAGGAAAAATTGAATCTGCATTTTGCAATCTCACAATATTAGTTCCAACACCATGCAATACTGGAATTGAAGCGTGGGTGAATCTAGAAGAAGAGAAAATGGCTATGCTAGAACAAGATCCCCAGTTTCTAGCACTAGCATCTCCAAAGACGCTAATTTCAAGAAATTGA

Protein Analysis

456

Amino Acids

51.33

Weight (kDa)

5.58

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 442 3.7e-60 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 230
AccIII TCCGGA 1 cut(s) 440
AclWI GGATC 2 cut(s) 1006, 1310
AcuI CTGAAG 2 cut(s) 53, 588
AfaI GTAC 2 cut(s) 218, 811
AfiI CCNNNNNNNGG 2 cut(s) 230, 413
AflII CTTAAG 3 cut(s) 184, 853, 965
AjnI CCWGG 3 cut(s) 90, 512, 840
AloI GAACNNNNNNTCC 2 cut(s) 1081, 1113
AluBI AGCT 7 cut(s) 53, 100, 179, 475, 934, 970, 1137
AluI AGCT 7 cut(s) 53, 100, 179, 475, 934, 970, 1137
Alw26I GTCTC 4 cut(s) 197, 566, 869, 1025
AlwI GGATC 2 cut(s) 1006, 1310
Ama87I CYCGRG 3 cut(s) 294, 792, 1132
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 3 cut(s) 750, 795, 921
ApaI GGGCCC 1 cut(s) 799
ApeKI GCWGC 3 cut(s) 572, 785, 836
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
AspS9I GGNCC 3 cut(s) 94, 795, 796
AsuHPI GGTGA 1 cut(s) 1288
AvaI CYCGRG 3 cut(s) 294, 792, 1132
AvaII GGWCC 1 cut(s) 94
BaeGI GKGCMC 3 cut(s) 732, 799, 919
BanII GRGCYC 1 cut(s) 799
BarI GAAGNNNNNNTAC 2 cut(s) 534, 566
BauI CACGAG 2 cut(s) 741, 1113
BbsI GAAGAC 2 cut(s) 148, 451
BbvI GCAGC 3 cut(s) 584, 772, 848
BccI CCATC 7 cut(s) 47, 55, 476, 619, 745, 1117, 1164
BceAI ACGGC 2 cut(s) 507, 737
BciT130I CCWGG 3 cut(s) 92, 514, 842
BcoDI GTCTC 4 cut(s) 197, 566, 869, 1025
BfaI CTAG 8 cut(s) 176, 558, 929, 1076, 1283, 1307, 1328, 1334
BfmI CTRYAG 1 cut(s) 831
BfrI CTTAAG 3 cut(s) 184, 853, 965
BisI GCNGC 3 cut(s) 573, 786, 837
BlsI GCNGC 3 cut(s) 574, 787, 838
Bme1390I CCNGG 3 cut(s) 92, 514, 842
Bme18I GGWCC 1 cut(s) 94
BmeT110I CYCGRG 3 cut(s) 294, 792, 1132
BmgT120I GGNCC 3 cut(s) 94, 795, 796
BmiI GGNNCC 2 cut(s) 797, 1107
BmrFI CCNGG 3 cut(s) 92, 514, 842
BmrI ACTGGG 1 cut(s) 1315
BmsI GCATC 1 cut(s) 1346
BmuI ACTGGG 1 cut(s) 1315
BpiI GAAGAC 2 cut(s) 148, 451
BpmI CTGGAG 1 cut(s) 93
BpuEI CTTGAG 2 cut(s) 308, 491
Bsa29I ATCGAT 2 cut(s) 258, 480
BsaJI CCNNGG 3 cut(s) 123, 513, 840
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 866, 896
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 413
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 4 cut(s) 725, 763, 1264, 1321
Bse3DI GCAATG 1 cut(s) 575
BseAI TCCGGA 1 cut(s) 440
BseBI CCWGG 3 cut(s) 92, 514, 842
BseCI ATCGAT 2 cut(s) 258, 480
BseDI CCNNGG 3 cut(s) 123, 513, 840
BseGI GGATG 2 cut(s) 553, 1175
BseLI CCNNNNNNNGG 2 cut(s) 230, 413
BseMI GCAATG 1 cut(s) 575
BseNI ACTGG 4 cut(s) 725, 763, 1264, 1321
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 3 cut(s) 732, 799, 919
BseXI GCAGC 3 cut(s) 584, 772, 848
BshFI GGCC 3 cut(s) 752, 797, 923
BshVI ATCGAT 2 cut(s) 258, 480
BsiHKCI CYCGRG 3 cut(s) 294, 792, 1132
BsiSI CCGG 3 cut(s) 441, 618, 645
BslFI GGGAC 2 cut(s) 104, 263
BslI CCNNNNNNNGG 2 cut(s) 230, 413
BsmAI GTCTC 4 cut(s) 197, 566, 869, 1025
BsmFI GGGAC 2 cut(s) 104, 263
BsnI GGCC 3 cut(s) 752, 797, 923
BsoBI CYCGRG 3 cut(s) 294, 792, 1132
Bsp120I GGGCCC 1 cut(s) 795
Bsp1286I GDGCHC 3 cut(s) 732, 799, 919
Bsp13I TCCGGA 1 cut(s) 440
Bsp143I GATC 3 cut(s) 961, 998, 1315
Bsp19I CCATGG 1 cut(s) 123
BspANI GGCC 3 cut(s) 752, 797, 923
BspDI ATCGAT 2 cut(s) 258, 480
BspEI TCCGGA 1 cut(s) 440
BspLI GGNNCC 2 cut(s) 797, 1107
BspPI GGATC 2 cut(s) 1006, 1310
BspQI GCTCTTC 1 cut(s) 710
BspTI CTTAAG 3 cut(s) 184, 853, 965
BsrDI GCAATG 1 cut(s) 575
BsrFI RCCGGY 1 cut(s) 617
BsrI ACTGG 4 cut(s) 725, 763, 1264, 1321
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 3 cut(s) 123, 513, 840
BssMI GATC 3 cut(s) 961, 998, 1315
BssSI CACGAG 2 cut(s) 741, 1113
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 2 cut(s) 741, 1113
Bst2UI CCWGG 3 cut(s) 92, 514, 842
Bst4CI ACNGT 3 cut(s) 500, 880, 1019
Bst6I CTCTTC 3 cut(s) 264, 710, 1284
BstAFI CTTAAG 3 cut(s) 184, 853, 965
BstC8I GCNNGC 1 cut(s) 722
BstDEI CTNAG 1 cut(s) 323
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 2 cut(s) 553, 1175
BstKTI GATC 3 cut(s) 964, 1001, 1318
BstMAI GTCTC 4 cut(s) 197, 566, 869, 1025
BstMBI GATC 3 cut(s) 961, 998, 1315
BstMWI GCNNNNNNNGC 2 cut(s) 794, 842
BstNI CCWGG 3 cut(s) 92, 514, 842
BstSCI CCNGG 3 cut(s) 90, 512, 840
BstSFI CTRYAG 1 cut(s) 831
BstSLI GKGCMC 3 cut(s) 732, 799, 919
BstV1I GCAGC 3 cut(s) 584, 772, 848
BstV2I GAAGAC 2 cut(s) 148, 451
BstX2I RGATCY 2 cut(s) 998, 1315
BstYI RGATCY 2 cut(s) 998, 1315
Bsu15I ATCGAT 2 cut(s) 258, 480
BsuRI GGCC 3 cut(s) 752, 797, 923
BsuTUI ATCGAT 2 cut(s) 258, 480
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 2 cut(s) 553, 1175
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 5 cut(s) 732, 756, 852, 885, 1024
Cac8I GCNNGC 1 cut(s) 722
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 3 cut(s) 94, 795, 796
ClaI ATCGAT 2 cut(s) 258, 480
CseI GACGC 1 cut(s) 1357
Csp6I GTAC 2 cut(s) 217, 810
CviAII CATG 6 cut(s) 124, 152, 310, 871, 1185, 1251
CviQI GTAC 2 cut(s) 217, 810
DdeI CTNAG 1 cut(s) 323
DpnI GATC 3 cut(s) 963, 1000, 1317
DpnII GATC 3 cut(s) 961, 998, 1315
Eam1104I CTCTTC 3 cut(s) 264, 710, 1284
EarI CTCTTC 3 cut(s) 264, 710, 1284
Eco130I CCWWGG 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 799
Eco32I GATATC 1 cut(s) 547
Eco47I GGWCC 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 53, 588
Eco88I CYCGRG 3 cut(s) 294, 792, 1132
EcoO109I RGGNCCY 1 cut(s) 796
EcoRII CCWGG 3 cut(s) 90, 512, 840
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 123
EcoT38I GRGCYC 1 cut(s) 799
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 6 cut(s) 127, 155, 313, 874, 1188, 1254
FaqI GGGAC 2 cut(s) 104, 263
FatI CATG 6 cut(s) 123, 151, 309, 870, 1184, 1250
Fnu4HI GCNGC 3 cut(s) 573, 786, 837
FokI GGATG 2 cut(s) 540, 1182
FriOI GRGCYC 1 cut(s) 799
Fsp4HI GCNGC 3 cut(s) 573, 786, 837
FspBI CTAG 8 cut(s) 176, 558, 929, 1076, 1283, 1307, 1328, 1334
GluI GCNGC 3 cut(s) 573, 786, 837
GsuI CTGGAG 1 cut(s) 93
HaeIII GGCC 3 cut(s) 752, 797, 923
HapII CCGG 3 cut(s) 441, 618, 645
HgaI GACGC 1 cut(s) 1357
Hin1II CATG 6 cut(s) 127, 155, 313, 874, 1188, 1254
HincII GTYRAC 2 cut(s) 427, 985
HindII GTYRAC 2 cut(s) 427, 985
HinfI GANTC 4 cut(s) 205, 437, 1211, 1279
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 3 cut(s) 441, 618, 645
HphI GGTGA 1 cut(s) 1288
Hpy166II GTNNAC 3 cut(s) 427, 850, 985
Hpy188I TCNGA 6 cut(s) 202, 333, 394, 568, 624, 1086
Hpy8I GTNNAC 3 cut(s) 427, 850, 985
HpyAV CCTTC 1 cut(s) 228
HpyCH4III ACNGT 3 cut(s) 500, 880, 1019
HpyCH4V TGCA 5 cut(s) 683, 1199, 1217, 1224, 1254
HpyF10VI GCNNNNNNNGC 2 cut(s) 794, 842
HpyF3I CTNAG 1 cut(s) 323
Hsp92II CATG 6 cut(s) 127, 155, 313, 874, 1188, 1254
Kpn2I TCCGGA 1 cut(s) 440
KspAI GTTAAC 1 cut(s) 985
Kzo9I GATC 3 cut(s) 961, 998, 1315
LguI GCTCTTC 1 cut(s) 710
Lsp1109I GCAGC 3 cut(s) 584, 772, 848
LweI GCATC 1 cut(s) 1346
MaeI CTAG 8 cut(s) 176, 558, 929, 1076, 1283, 1307, 1328, 1334
MaeIII GTNAC 5 cut(s) 305, 401, 754, 974, 1013
MalI GATC 3 cut(s) 963, 1000, 1317
MboI GATC 3 cut(s) 961, 998, 1315
MflI RGATCY 2 cut(s) 998, 1315
MhlI GDGCHC 3 cut(s) 732, 799, 919
MluCI AATT 9 cut(s) 9, 24, 301, 699, 1144, 1206, 1263, 1353, 1363
MlyI GAGTC 2 cut(s) 199, 431
MmeI TCCRAC 2 cut(s) 864, 1268
MroI TCCGGA 1 cut(s) 440
MseI TTAA 5 cut(s) 185, 854, 867, 966, 984
MspCI CTTAAG 3 cut(s) 184, 853, 965
MspI CCGG 3 cut(s) 441, 618, 645
MspR9I CCNGG 3 cut(s) 92, 514, 842
MvaI CCWGG 3 cut(s) 92, 514, 842
MwoI GCNNNNNNNGC 2 cut(s) 794, 842
NcoI CCATGG 1 cut(s) 123
NdeII GATC 3 cut(s) 961, 998, 1315
NlaIII CATG 6 cut(s) 127, 155, 313, 874, 1188, 1254
NlaIV GGNNCC 2 cut(s) 797, 1107
NmuCI GTSAC 3 cut(s) 305, 754, 974
PaeR7I CTCGAG 2 cut(s) 294, 1132
PciSI GCTCTTC 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 535
PfeI GAWTC 2 cut(s) 1211, 1279
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 3 cut(s) 574, 787, 838
PleI GAGTC 2 cut(s) 199, 431
PpsI GAGTC 2 cut(s) 199, 431
Psp6I CCWGG 3 cut(s) 90, 512, 840
PspGI CCWGG 3 cut(s) 90, 512, 840
PspN4I GGNNCC 2 cut(s) 797, 1107
PspOMI GGGCCC 1 cut(s) 795
PspPI GGNCC 3 cut(s) 94, 795, 796
PspXI VCTCGAGB 1 cut(s) 294
PsuI RGATCY 2 cut(s) 998, 1315
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 2 cut(s) 218, 811
RsaNI GTAC 2 cut(s) 217, 810
SapI GCTCTTC 1 cut(s) 710
SaqAI TTAA 5 cut(s) 185, 854, 867, 966, 984
SatI GCNGC 3 cut(s) 573, 786, 837
Sau3AI GATC 3 cut(s) 961, 998, 1315
Sau96I GGNCC 3 cut(s) 94, 795, 796
SchI GAGTC 2 cut(s) 199, 431
ScrFI CCNGG 3 cut(s) 92, 514, 842
SduI GDGCHC 3 cut(s) 732, 799, 919
SfaNI GCATC 1 cut(s) 1346
SfcI CTRYAG 1 cut(s) 831
Sfr274I CTCGAG 2 cut(s) 294, 1132
SinI GGWCC 1 cut(s) 94
SlaI CTCGAG 2 cut(s) 294, 1132
SmlI CTYRAG 7 cut(s) 184, 287, 294, 506, 853, 965, 1132
SmoI CTYRAG 7 cut(s) 184, 287, 294, 506, 853, 965, 1132
Sse9I AATT 9 cut(s) 9, 24, 301, 699, 1144, 1206, 1263, 1353, 1363
SspI AATATT 1 cut(s) 1236
SspMI CTAG 8 cut(s) 176, 558, 929, 1076, 1283, 1307, 1328, 1334
StyD4I CCNGG 3 cut(s) 90, 512, 840
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 500, 880, 1019
TaqI TCGA 6 cut(s) 258, 295, 339, 480, 529, 1133
TasI AATT 9 cut(s) 9, 24, 301, 699, 1144, 1206, 1263, 1353, 1363
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 2 cut(s) 1211, 1279
Tru1I TTAA 5 cut(s) 185, 854, 867, 966, 984
Tru9I TTAA 5 cut(s) 185, 854, 867, 966, 984
TscAI CASTG 5 cut(s) 732, 763, 852, 885, 1024
TseFI GTSAC 3 cut(s) 305, 754, 974
TseI GCWGC 3 cut(s) 572, 785, 836
Tsp45I GTSAC 3 cut(s) 305, 754, 974
TspDTI ATGAA 2 cut(s) 451, 723
TspGWI ACGGA 1 cut(s) 877
TspRI CASTG 5 cut(s) 732, 763, 852, 885, 1024
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 230
Vha464I CTTAAG 3 cut(s) 184, 853, 965
VpaK11BI GGWCC 1 cut(s) 94
XbaI TCTAGA 2 cut(s) 1075, 1282
XhoI CTCGAG 2 cut(s) 294, 1132
XspI CTAG 8 cut(s) 176, 558, 929, 1076, 1283, 1307, 1328, 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.