RchiOBHm_Chr2g0101491

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006417
Physical Location & Seq
Forward (+)
13116810 .. 13119783
2974 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47609

Sequence Viewer

Length: 1374 bp
ATGGAGAAAATTGAGGTCAGTATTATTTCCAGAGACACCATTAAACCATCAGCTGCTTCCTCTTCACTACACCCTTACAAGCTTTCCATCATCGATCAGTTCACTCCCACAATGTATTTCCCAGTTATATTCTTCTACCCCATTACTGACCGTGTCTTCAATCTTCCTCAAACCTTAACCGACTTGAAAAACACTGTTTCCCAGGCTCTCACTTTGTACTATCCACTCTCCGGAAGGATAAAAAACAACCTATACATTGATGATTTCGAAGCAGGCATCCCCTTCCTTGAGGCCCGAGTGAATTTTCACATGATTGATTTTCTAAGGCTTCCGAAAATCGAGTGGCTAAATGAGTTTGTTCCAATAGCTCCATATCGCAAGGAAACAATATCTGAGTTTCTTCCCTTGCTTGGAATTCAAGTAAACATTTTCGACTCTGGAATAGCAATCGGTGTCTCTTTCTCTCACAAGATCAACGATGGCAAAACGGCAAGCTGTTTTCTCAAGTCCTGGGTTGCTATTTTTCGTGGGTATCGTAACAAAATCATACATCCTAATCTCTCTCAAGCTGCATTACTTTTGCCATCGAGGGATGACTTGCCTGAAAAGTATGTAGCTATGATGGAAAGGTTGTGGTTTGGCGAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCGCACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCTCGGGCATTATCATCAGGTACTTCAACAAGATTTTCCGTAGCGTCTCAAACAGTGAACTTAAGGTCAAAAATGAACATGAAAACGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTTCGGCACAGCTAGATCTAAATGATACAGCACCAGGGAGCAGTGATCTTAAGTTGTGTGACTTGGTTAACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTTTGAAGGGTAAAGAGGGATATGGAGGCATGTGTGATTTGCTAGATTTCATGGAAGAGGGGAGTTCTGTAGAACCAGCACCAGAGTTTTATTCATTCTCAAGCTGGACTAGATTTTTTGACCAAGTTGATTTTGGATGGGGGAGGCCATCTTGGGTTGGAGTCTCGGGGAGTGTTGGAAATAGAAATTTCACAACATTCGTTGAAACACAATGCGATGACGGAATTGAAGCGTGGGTGACTCTAGATGAAAAACAAATGGCTATGCTAGAACAAGATCCACAGTTTTTAGCATTTGCATCTCCAAACCCCCGAATTTCAATAACCTCTTCAGTTGGTATTGATTAA

Protein Analysis

457

Amino Acids

51.52

Weight (kDa)

5.38

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 438 1.1e-61 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 1 cut(s) 693
AclWI GGATC 1 cut(s) 1298
AcsI RAATTY 5 cut(s) 301, 414, 1004, 1213, 1341
AcuI CTGAAG 1 cut(s) 1341
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 3 cut(s) 218, 722, 808
AfiI CCNNNNNNNGG 2 cut(s) 230, 410
AflII CTTAAG 2 cut(s) 847, 959
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 9 cut(s) 160, 187, 419, 701, 813, 1033, 1232, 1256, 1347
AjnI CCWGG 3 cut(s) 201, 509, 943
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 8 cut(s) 53, 82, 368, 495, 569, 617, 922, 1131
AluI AGCT 8 cut(s) 53, 82, 368, 495, 569, 617, 922, 1131
Alw26I GTCTC 5 cut(s) 27, 460, 837, 1019, 1195
AlwI GGATC 1 cut(s) 1298
Ama87I CYCGRG 3 cut(s) 294, 789, 1192
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 3 cut(s) 291, 747, 1172
ApeKI GCWGC 3 cut(s) 53, 569, 782
ApoI RAATTY 5 cut(s) 301, 414, 1004, 1213, 1341
AspS9I GGNCC 2 cut(s) 292, 748
AsuHPI GGTGA 1 cut(s) 1276
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 3 cut(s) 294, 789, 1192
BaeGI GKGCMC 1 cut(s) 729
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 40, 556, 769
BccI CCATC 8 cut(s) 55, 95, 473, 592, 616, 742, 1158, 1183
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 3 cut(s) 203, 511, 945
BcoDI GTCTC 5 cut(s) 27, 460, 837, 1019, 1195
BfaI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
BfmI CTRYAG 1 cut(s) 1095
BfrI CTTAAG 2 cut(s) 847, 959
BglII AGATCT 1 cut(s) 925
BisI GCNGC 3 cut(s) 54, 570, 783
BlsI GCNGC 3 cut(s) 55, 571, 784
Bme1390I CCNGG 3 cut(s) 203, 511, 945
BmeT110I CYCGRG 3 cut(s) 294, 789, 1192
BmgT120I GGNCC 2 cut(s) 292, 748
BmrFI CCNGG 3 cut(s) 203, 511, 945
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 3 cut(s) 285, 667, 1334
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 4 cut(s) 308, 488, 549, 1111
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 2 cut(s) 724, 740
BsaJI CCNNGG 3 cut(s) 201, 510, 944
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 410
Bse1I ACTGG 2 cut(s) 122, 760
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 3 cut(s) 203, 511, 945
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 3 cut(s) 201, 510, 944
BseGI GGATG 4 cut(s) 276, 550, 598, 1169
BseLI CCNNNNNNNGG 2 cut(s) 230, 410
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 122, 760
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 3 cut(s) 40, 556, 769
BshFI GGCC 3 cut(s) 293, 749, 1174
BshVI ATCGAT 1 cut(s) 93
BsiHKCI CYCGRG 3 cut(s) 294, 789, 1192
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 230, 410
BsmAI GTCTC 5 cut(s) 27, 460, 837, 1019, 1195
BsmBI CGTCTC 1 cut(s) 837
BsnI GGCC 3 cut(s) 293, 749, 1174
BsoBI CYCGRG 3 cut(s) 294, 789, 1192
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 729
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 5 cut(s) 94, 471, 925, 955, 1303
BspACI CCGC 1 cut(s) 693
BspANI GGCC 3 cut(s) 293, 749, 1174
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 93
BspEI TCCGGA 1 cut(s) 230
BspPI GGATC 1 cut(s) 1298
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 267
BspTI CTTAAG 2 cut(s) 847, 959
BsrI ACTGG 2 cut(s) 122, 760
BssECI CCNNGG 3 cut(s) 201, 510, 944
BssMI GATC 5 cut(s) 94, 471, 925, 955, 1303
Bst2UI CCWGG 3 cut(s) 203, 511, 945
Bst4CI ACNGT 5 cut(s) 152, 196, 841, 1013, 1311
Bst6I CTCTTC 4 cut(s) 67, 707, 1077, 1360
BstAFI CTTAAG 2 cut(s) 847, 959
BstBAI YACGTR 2 cut(s) 724, 740
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 274, 493
BstDEI CTNAG 2 cut(s) 323, 393
BstF5I GGATG 4 cut(s) 276, 550, 598, 1169
BstKTI GATC 5 cut(s) 97, 474, 928, 958, 1306
BstMAI GTCTC 5 cut(s) 27, 460, 837, 1019, 1195
BstMBI GATC 5 cut(s) 94, 471, 925, 955, 1303
BstMWI GCNNNNNNNGC 2 cut(s) 692, 791
BstNI CCWGG 3 cut(s) 203, 511, 945
BstNSI RCATGY 1 cut(s) 1060
BstSCI CCNGG 3 cut(s) 201, 509, 943
BstSFI CTRYAG 1 cut(s) 1095
BstSLI GKGCMC 1 cut(s) 729
BstV1I GCAGC 3 cut(s) 40, 556, 769
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 925, 1303
BstYI RGATCY 2 cut(s) 925, 1303
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 3 cut(s) 293, 749, 1174
BsuTUI ATCGAT 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 1257
BtsCI GGATG 4 cut(s) 276, 550, 598, 1169
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 5 cut(s) 192, 753, 846, 958, 1018
Cac8I GCNNGC 2 cut(s) 274, 493
Cfr13I GGNCC 2 cut(s) 292, 748
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 819
Csp6I GTAC 3 cut(s) 217, 721, 807
CviAII CATG 4 cut(s) 310, 865, 1057, 1078
CviQI GTAC 3 cut(s) 217, 721, 807
DdeI CTNAG 2 cut(s) 323, 393
DpnI GATC 5 cut(s) 96, 473, 927, 957, 1305
DpnII GATC 5 cut(s) 94, 471, 925, 955, 1303
Eam1104I CTCTTC 4 cut(s) 67, 707, 1077, 1360
EarI CTCTTC 4 cut(s) 67, 707, 1077, 1360
Eco57I CTGAAG 1 cut(s) 1341
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 3 cut(s) 294, 789, 1192
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 3 cut(s) 201, 509, 943
Esp3I CGTCTC 1 cut(s) 837
FaeI CATG 4 cut(s) 313, 868, 1060, 1081
FatI CATG 4 cut(s) 309, 864, 1056, 1077
Fnu4HI GCNGC 3 cut(s) 54, 570, 783
FokI GGATG 4 cut(s) 263, 537, 605, 1176
Fsp4HI GCNGC 3 cut(s) 54, 570, 783
FspBI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
GluI GCNGC 3 cut(s) 54, 570, 783
HaeIII GGCC 3 cut(s) 293, 749, 1174
HapII CCGG 1 cut(s) 231
HgaI GACGC 1 cut(s) 819
Hin1II CATG 4 cut(s) 313, 868, 1060, 1081
HincII GTYRAC 1 cut(s) 979
HindII GTYRAC 1 cut(s) 979
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 4 cut(s) 434, 992, 1188, 1267
HpaI GTTAAC 1 cut(s) 979
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 1276
Hpy166II GTNNAC 4 cut(s) 102, 424, 844, 979
Hpy188I TCNGA 2 cut(s) 333, 394
Hpy188III TCNNGA 6 cut(s) 30, 231, 438, 701, 766, 1271
Hpy8I GTNNAC 4 cut(s) 102, 424, 844, 979
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 4 cut(s) 228, 292, 701, 1027
HpyCH4III ACNGT 5 cut(s) 152, 196, 841, 1013, 1311
HpyCH4IV ACGT 3 cut(s) 723, 739, 875
HpyCH4V TGCA 2 cut(s) 572, 1325
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 791
HpyF3I CTNAG 2 cut(s) 323, 393
HpySE526I ACGT 3 cut(s) 723, 739, 875
Hsp92II CATG 4 cut(s) 313, 868, 1060, 1081
Kpn2I TCCGGA 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 979
Kzo9I GATC 5 cut(s) 94, 471, 925, 955, 1303
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 2 cut(s) 373, 948
Lsp1109I GCAGC 3 cut(s) 40, 556, 769
LweI GCATC 3 cut(s) 285, 667, 1334
MaeI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
MaeII ACGT 3 cut(s) 723, 739, 875
MaeIII GTNAC 4 cut(s) 536, 655, 968, 1264
MalI GATC 5 cut(s) 96, 473, 927, 957, 1305
MboI GATC 5 cut(s) 94, 471, 925, 955, 1303
MboII GAAGA 8 cut(s) 54, 124, 148, 155, 392, 724, 1094, 1347
MflI RGATCY 2 cut(s) 925, 1303
MhlI GDGCHC 1 cut(s) 729
MluCI AATT 7 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341
MlyI GAGTC 3 cut(s) 428, 1197, 1261
MmeI TCCRAC 3 cut(s) 858, 1165, 1183
MroI TCCGGA 1 cut(s) 230
MseI TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 2 cut(s) 847, 959
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 3 cut(s) 203, 511, 945
MvaI CCWGG 3 cut(s) 203, 511, 945
MwoI GCNNNNNNNGC 2 cut(s) 692, 791
NdeII GATC 5 cut(s) 94, 471, 925, 955, 1303
NlaIII CATG 4 cut(s) 313, 868, 1060, 1081
NmuCI GTSAC 2 cut(s) 968, 1264
NspI RCATGY 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PfeI GAWTC 1 cut(s) 992
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 55, 571, 784
PleI GAGTC 3 cut(s) 428, 1196, 1261
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 3 cut(s) 428, 1196, 1261
Ppu21I YACGTR 2 cut(s) 724, 740
Psp6I CCWGG 3 cut(s) 201, 509, 943
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 3 cut(s) 201, 509, 943
PspPI GGNCC 2 cut(s) 292, 748
PsuI RGATCY 2 cut(s) 925, 1303
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 3 cut(s) 218, 722, 808
RsaNI GTAC 3 cut(s) 217, 721, 807
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
SatI GCNGC 3 cut(s) 54, 570, 783
Sau3AI GATC 5 cut(s) 94, 471, 925, 955, 1303
Sau96I GGNCC 2 cut(s) 292, 748
SchI GAGTC 3 cut(s) 428, 1197, 1261
ScrFI CCNGG 3 cut(s) 203, 511, 945
SduI GDGCHC 1 cut(s) 729
SfaNI GCATC 3 cut(s) 285, 667, 1334
SfcI CTRYAG 1 cut(s) 1095
SfuI TTCGAA 1 cut(s) 267
SmlI CTYRAG 6 cut(s) 287, 503, 564, 847, 959, 1126
SmoI CTYRAG 6 cut(s) 287, 503, 564, 847, 959, 1126
Sse9I AATT 7 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341
SsiI CCGC 1 cut(s) 693
SspMI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
StyD4I CCNGG 3 cut(s) 201, 509, 943
TaaI ACNGT 5 cut(s) 152, 196, 841, 1013, 1311
TaiI ACGT 3 cut(s) 726, 742, 878
TaqI TCGA 5 cut(s) 93, 267, 339, 432, 587
TasI AATT 7 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 992
Tru1I TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
Tru9I TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
TscAI CASTG 5 cut(s) 199, 760, 846, 958, 1018
TseFI GTSAC 2 cut(s) 968, 1264
TseI GCWGC 3 cut(s) 53, 569, 782
Tsp45I GTSAC 2 cut(s) 968, 1264
TspDTI ATGAA 7 cut(s) 720, 875, 881, 1005, 1066, 1110, 1290
TspGWI ACGGA 2 cut(s) 814, 1263
TspRI CASTG 5 cut(s) 199, 760, 846, 958, 1018
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 2 cut(s) 847, 959
XapI RAATTY 5 cut(s) 301, 414, 1004, 1213, 1341
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 1 cut(s) 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 1157
XspI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.