Rw2G011470

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0006417
Physical Location & Seq
Forward (+)
11593578 .. 11595559
1982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G011470.1

Sequence Viewer

Length: 1353 bp
ATGGAGAAAATTGAGGTCAGTATTATTTCCAGAGACACCATTAAACCATCAGCTGCTTCCTCTTCACTACACCCTTACAAGCTTTCCATCATCGATCAGCTCACTCCCACAATGTATTTCCCAGTTATATTCTTCTACCCCATTACTGACCGTGTCTTCAATCTTCCTCAAACCTTAACCGACTTGAAAACCACTGTTTCCCAGGCTCTCACTTTGTACTATCCACTCTCCGGGAGGATAAAAAACAACCTATACATTGATGATTTCGAAGCAGGCATCCCCTACCTCGAGGCCCGAGTGAATTTTCACATGATTGATTTTCTAAGGCTTCCGAAAATCGAGTGGCTAAATGAGTTTGTTCCAATGGCTCCATATCGCAAGGAAACAATATCTGAGTTTCTTCCCTTGCTTGGAATTCAAGTAAACATTTTCGACTCTGGAATAGCAATCGGTGTCTCTTTCTCTCACAAGATCAACGATGGCAAAACGGCAAGCTGTTTTCTCAAGTCCTGGGTTGCTATTTTTCGTGGGTATCGTAACAAAATCATACATCCTAATCTCTCTCAAGCTGCATTACTTTTGCCATCGAGGGATGACTTGCCTGAAAAGTACGTAGCTATGATGGAAAGGTTGTGGTTTGGCGAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCGCACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCCGCTAATTCTCGGGCATTATCATCAGGTAAATCAACAGTGAACTTAAGGTCAAAAATGAACATGAAAATGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTTCGGCACAGCTAGATCTAAATGATACAGCACCAGGGAGAAGTGATCTTAAGTTGTGTGACTTGGTTGACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTTTGAAGGGTAAAGAGGGATATGGAGGCATGTGTGATTTGCTAGATTTCATGGAAGAGGGGAGTTCTGTAGAACCAGCACCAGAGTTTTATTCATTCTCAAGCTGGACTAGATTTTTTGACCAAGTTGATTTTGGATGGGGGAGGCCATCTTGGGTTGGAGTCTCGGGGAGAGTTGGAAATAGAAATTTCACAACATTCGTTGAAACACAATCCGATAACGGAATTGAAGCGTGGGTGACTCTAGATGAAAAACAAATGGCTATGCTAGAACAAGATCCACAGTTTTTAGCATTTGCATCTCCAAACCCCCCAATTTCAATAACCTCTTCAGTTGGTATTGATTAA

Protein Analysis

450

Amino Acids

50.86

Weight (kDa)

5.48

Isoelectric Point (pI)

38.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 431 5.7e-59 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 287
AciI CCGC 2 cut(s) 693, 783
AclWI GGATC 1 cut(s) 1277
AcsI RAATTY 4 cut(s) 301, 414, 983, 1192
AcuI CTGAAG 1 cut(s) 1320
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 3 cut(s) 218, 611, 722
AfiI CCNNNNNNNGG 2 cut(s) 230, 410
AflII CTTAAG 2 cut(s) 826, 938
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 8 cut(s) 160, 187, 419, 701, 1012, 1211, 1235, 1326
AjnI CCWGG 3 cut(s) 201, 509, 922
AjuI GAANNNNNNNTTGG 2 cut(s) 839, 871
AluBI AGCT 8 cut(s) 53, 82, 100, 495, 569, 617, 901, 1110
AluI AGCT 8 cut(s) 53, 82, 100, 495, 569, 617, 901, 1110
Alw26I GTCTC 4 cut(s) 27, 460, 998, 1174
AlwI GGATC 1 cut(s) 1277
Ama87I CYCGRG 4 cut(s) 287, 294, 792, 1171
AoxI GGCC 3 cut(s) 291, 747, 1151
ApeKI GCWGC 2 cut(s) 53, 569
ApoI RAATTY 4 cut(s) 301, 414, 983, 1192
AspS9I GGNCC 2 cut(s) 292, 748
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 1255
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 4 cut(s) 287, 294, 792, 1171
BaeGI GKGCMC 1 cut(s) 729
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 2 cut(s) 40, 556
BccI CCATC 8 cut(s) 55, 95, 473, 592, 616, 742, 1137, 1162
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 3 cut(s) 203, 511, 924
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 4 cut(s) 27, 460, 998, 1174
BfaI CTAG 5 cut(s) 902, 1049, 1116, 1250, 1274
BfmI CTRYAG 1 cut(s) 1074
BfrI CTTAAG 2 cut(s) 826, 938
BglII AGATCT 1 cut(s) 904
BisI GCNGC 3 cut(s) 54, 570, 783
BlsI GCNGC 3 cut(s) 55, 571, 784
Bme1390I CCNGG 4 cut(s) 203, 232, 511, 924
BmeT110I CYCGRG 4 cut(s) 287, 294, 792, 1171
BmgT120I GGNCC 2 cut(s) 292, 748
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 4 cut(s) 203, 232, 511, 924
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 3 cut(s) 285, 667, 1313
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 488, 549, 1090
BpuMI CCSGG 1 cut(s) 232
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 3 cut(s) 613, 724, 740
BsaJI CCNNGG 3 cut(s) 201, 510, 923
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 410
Bse1I ACTGG 2 cut(s) 122, 760
BseBI CCWGG 3 cut(s) 203, 511, 924
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 3 cut(s) 201, 510, 923
BseGI GGATG 4 cut(s) 276, 550, 598, 1148
BseLI CCNNNNNNNGG 2 cut(s) 230, 410
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 122, 760
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 2 cut(s) 40, 556
BshFI GGCC 3 cut(s) 293, 749, 1153
BshVI ATCGAT 1 cut(s) 93
BsiHKCI CYCGRG 4 cut(s) 287, 294, 792, 1171
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 230, 410
BsmAI GTCTC 4 cut(s) 27, 460, 998, 1174
BsnI GGCC 3 cut(s) 293, 749, 1153
BsoBI CYCGRG 4 cut(s) 287, 294, 792, 1171
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 729
Bsp143I GATC 5 cut(s) 94, 471, 904, 934, 1282
BspACI CCGC 2 cut(s) 693, 783
BspANI GGCC 3 cut(s) 293, 749, 1153
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 93
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 1 cut(s) 1277
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 267
BspTI CTTAAG 2 cut(s) 826, 938
BsrI ACTGG 2 cut(s) 122, 760
BssECI CCNNGG 3 cut(s) 201, 510, 923
BssMI GATC 5 cut(s) 94, 471, 904, 934, 1282
Bst2UI CCWGG 3 cut(s) 203, 511, 924
Bst4CI ACNGT 5 cut(s) 152, 196, 820, 992, 1290
Bst6I CTCTTC 4 cut(s) 67, 707, 1056, 1339
BstAFI CTTAAG 2 cut(s) 826, 938
BstBAI YACGTR 3 cut(s) 613, 724, 740
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 274, 493
BstDEI CTNAG 2 cut(s) 323, 393
BstF5I GGATG 4 cut(s) 276, 550, 598, 1148
BstKTI GATC 5 cut(s) 97, 474, 907, 937, 1285
BstMAI GTCTC 4 cut(s) 27, 460, 998, 1174
BstMBI GATC 5 cut(s) 94, 471, 904, 934, 1282
BstMWI GCNNNNNNNGC 1 cut(s) 692
BstNI CCWGG 3 cut(s) 203, 511, 924
BstNSI RCATGY 1 cut(s) 1039
BstSCI CCNGG 4 cut(s) 201, 230, 509, 922
BstSFI CTRYAG 1 cut(s) 1074
BstSLI GKGCMC 1 cut(s) 729
BstSNI TACGTA 1 cut(s) 613
BstV1I GCAGC 2 cut(s) 40, 556
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 904, 1282
BstYI RGATCY 2 cut(s) 904, 1282
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 3 cut(s) 293, 749, 1153
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 4 cut(s) 276, 550, 598, 1148
BtsIMutI CAGTG 4 cut(s) 192, 753, 825, 997
Cac8I GCNNGC 2 cut(s) 274, 493
Cfr13I GGNCC 2 cut(s) 292, 748
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 3 cut(s) 217, 610, 721
CviAII CATG 4 cut(s) 310, 844, 1036, 1057
CviQI GTAC 3 cut(s) 217, 610, 721
DdeI CTNAG 2 cut(s) 323, 393
DpnI GATC 5 cut(s) 96, 473, 906, 936, 1284
DpnII GATC 5 cut(s) 94, 471, 904, 934, 1282
Eam1104I CTCTTC 4 cut(s) 67, 707, 1056, 1339
EarI CTCTTC 4 cut(s) 67, 707, 1056, 1339
Eco105I TACGTA 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 1320
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 4 cut(s) 287, 294, 792, 1171
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 3 cut(s) 201, 509, 922
FaeI CATG 4 cut(s) 313, 847, 1039, 1060
FatI CATG 4 cut(s) 309, 843, 1035, 1056
Fnu4HI GCNGC 3 cut(s) 54, 570, 783
FokI GGATG 4 cut(s) 263, 537, 605, 1155
Fsp4HI GCNGC 3 cut(s) 54, 570, 783
FspBI CTAG 5 cut(s) 902, 1049, 1116, 1250, 1274
GluI GCNGC 3 cut(s) 54, 570, 783
HaeIII GGCC 3 cut(s) 293, 749, 1153
HapII CCGG 1 cut(s) 231
Hin1II CATG 4 cut(s) 313, 847, 1039, 1060
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 4 cut(s) 434, 971, 1167, 1246
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 1255
Hpy166II GTNNAC 3 cut(s) 424, 823, 958
Hpy188I TCNGA 3 cut(s) 333, 394, 1222
Hpy188III TCNNGA 5 cut(s) 30, 438, 701, 766, 1250
Hpy8I GTNNAC 3 cut(s) 424, 823, 958
HpyAV CCTTC 2 cut(s) 701, 1006
HpyCH4III ACNGT 5 cut(s) 152, 196, 820, 992, 1290
HpyCH4IV ACGT 4 cut(s) 612, 723, 739, 854
HpyCH4V TGCA 2 cut(s) 572, 1304
HpyF10VI GCNNNNNNNGC 1 cut(s) 692
HpyF3I CTNAG 2 cut(s) 323, 393
HpySE526I ACGT 4 cut(s) 612, 723, 739, 854
Hsp92II CATG 4 cut(s) 313, 847, 1039, 1060
Kzo9I GATC 5 cut(s) 94, 471, 904, 934, 1282
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 2 cut(s) 40, 556
LweI GCATC 3 cut(s) 285, 667, 1313
MaeI CTAG 5 cut(s) 902, 1049, 1116, 1250, 1274
MaeII ACGT 4 cut(s) 612, 723, 739, 854
MaeIII GTNAC 4 cut(s) 536, 655, 947, 1243
MalI GATC 5 cut(s) 96, 473, 906, 936, 1284
MboI GATC 5 cut(s) 94, 471, 904, 934, 1282
MboII GAAGA 8 cut(s) 54, 124, 148, 155, 392, 724, 1073, 1326
MflI RGATCY 2 cut(s) 904, 1282
MhlI GDGCHC 1 cut(s) 729
MluCI AATT 8 cut(s) 9, 301, 414, 787, 983, 1192, 1230, 1320
MlyI GAGTC 3 cut(s) 428, 1176, 1240
MmeI TCCRAC 3 cut(s) 837, 1144, 1162
MseI TTAA 6 cut(s) 42, 176, 827, 939, 987, 1351
MslI CAYNNNNRTG 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 2 cut(s) 826, 938
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 4 cut(s) 203, 232, 511, 924
MvaI CCWGG 3 cut(s) 203, 511, 924
MwoI GCNNNNNNNGC 1 cut(s) 692
NciI CCSGG 1 cut(s) 232
NdeII GATC 5 cut(s) 94, 471, 904, 934, 1282
NlaIII CATG 4 cut(s) 313, 847, 1039, 1060
NlaIV GGNNCC 1 cut(s) 369
NmuCI GTSAC 2 cut(s) 947, 1243
NspI RCATGY 1 cut(s) 1039
NspV TTCGAA 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PfeI GAWTC 1 cut(s) 971
PflFI GACNNNGTC 1 cut(s) 152
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 3 cut(s) 55, 571, 784
PleI GAGTC 3 cut(s) 428, 1175, 1240
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 3 cut(s) 428, 1175, 1240
Ppu21I YACGTR 3 cut(s) 613, 724, 740
Psp6I CCWGG 3 cut(s) 201, 509, 922
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 3 cut(s) 201, 509, 922
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 2 cut(s) 292, 748
PspXI VCTCGAGB 1 cut(s) 287
PsuI RGATCY 2 cut(s) 904, 1282
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 3 cut(s) 218, 611, 722
RsaNI GTAC 3 cut(s) 217, 610, 721
RseI CAYNNNNRTG 1 cut(s) 848
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 6 cut(s) 42, 176, 827, 939, 987, 1351
SatI GCNGC 3 cut(s) 54, 570, 783
Sau3AI GATC 5 cut(s) 94, 471, 904, 934, 1282
Sau96I GGNCC 2 cut(s) 292, 748
SchI GAGTC 3 cut(s) 428, 1176, 1240
ScrFI CCNGG 4 cut(s) 203, 232, 511, 924
SduI GDGCHC 1 cut(s) 729
SfaNI GCATC 3 cut(s) 285, 667, 1313
SfcI CTRYAG 1 cut(s) 1074
Sfr274I CTCGAG 1 cut(s) 287
SfuI TTCGAA 1 cut(s) 267
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 6 cut(s) 287, 503, 564, 826, 938, 1105
SmoI CTYRAG 6 cut(s) 287, 503, 564, 826, 938, 1105
SnaBI TACGTA 1 cut(s) 613
Sse9I AATT 8 cut(s) 9, 301, 414, 787, 983, 1192, 1230, 1320
SsiI CCGC 2 cut(s) 693, 783
SspMI CTAG 5 cut(s) 902, 1049, 1116, 1250, 1274
StyD4I CCNGG 4 cut(s) 201, 230, 509, 922
TaaI ACNGT 5 cut(s) 152, 196, 820, 992, 1290
TaiI ACGT 4 cut(s) 615, 726, 742, 857
TaqI TCGA 6 cut(s) 93, 267, 288, 339, 432, 587
TasI AATT 8 cut(s) 9, 301, 414, 787, 983, 1192, 1230, 1320
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 1 cut(s) 971
Tru1I TTAA 6 cut(s) 42, 176, 827, 939, 987, 1351
Tru9I TTAA 6 cut(s) 42, 176, 827, 939, 987, 1351
TscAI CASTG 4 cut(s) 199, 760, 825, 997
TseFI GTSAC 2 cut(s) 947, 1243
TseI GCWGC 2 cut(s) 53, 569
Tsp45I GTSAC 2 cut(s) 947, 1243
TspDTI ATGAA 7 cut(s) 720, 854, 860, 984, 1045, 1089, 1269
TspGWI ACGGA 1 cut(s) 1242
TspRI CASTG 4 cut(s) 199, 760, 825, 997
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 2 cut(s) 826, 938
XapI RAATTY 4 cut(s) 301, 414, 983, 1192
XbaI TCTAGA 1 cut(s) 1249
XceI RCATGY 1 cut(s) 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XhoI CTCGAG 1 cut(s) 287
XspI CTAG 5 cut(s) 902, 1049, 1116, 1250, 1274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.