Rroxscaffold_4G00294230

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006417
Physical Location & Seq
Reverse (-)
14378432 .. 14381235
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294230.1

Sequence Viewer

Length: 1389 bp
ATGGAGAAAATTGAGGTCAGTATCATTTCCAGAGACACCATCAAACCATCAGCTGCTTCCTCTTCACTACACCCTCACAAGCTTTCCATCATCGATCAAATCACTCCTACAATGTATGTCCCGGTTATATTCTTCTACCCCATTACTGACCCCGTCTTCAATCTTCCTCGAACCTTAACGGACTTAAAAAACACTCTTTCCCAGACTCTTACCTTGTACTATCCACTATCCGGAAGGGTCAAAAACAACTTATACATCGATGATTTTGAAGCGGGTGTCCCCTACCTTGAGGTCAGAGTAAATTGTAACATGATTGATTTTCTAAAGCTTCCAAAAACCGAGTGGCTAAATGAGTTTGTTCCAATCGAACCATATCGCAAGGAAACAATATCTGAGCTTCTCCCCTTGCTTGGAGTTCAAGTCAATATCTTCGACTATGGAATAGCAATTGGTGTCTCCCTTTGTCACAAGCTCATCGATGGCGAAACAGCAAACTGTTTTCTCAAGTCCTGGGTTGCTGCTTTTCGTGGGTGTCGTGACAAAATCATTCATCCGAATCTCTCTCAAGCAGCAGTACTTTTCCCACCGAGAGATGACTTGCCTGAAAAGTATGTAGCTCTGATGGAAAGGTGGTGCTTTTGCGAAAGAAATGTTGTTACAAGGAGATTTGTATTTAAGGCGAAAGCCATATCTGCACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCGAGCCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCCCGGGCATTATCATCAGGTACTTCAACAAGATTTTCTTTAGCAATCCAAGCGGTGAACTTAAGGTCACGAATGAACATGAAAACGACGTTGGACAATGCCATTGGAAATATCTTTTTGTGGGCTCCGGCATTCCTAGAGCTAAATTATACAACACCAGATAGTAGTGATCATAAGTTGTGTGACTTGGTTAACTTGCTCAAAGAATCTGTCAAAGAATATAACAGTGATTACTTGGAGACTTTAAAGGGTGAAAAGGGATACGGAGGTATGTGTGATTGGCTAGATTTGATGGAAGAGGGGAGTTCTATAGAACCAGCACTAGAGATTTATTCATTCTCGAGCTGGACTAGAATTTTTGACCAAGTTGATTTTGGATGGGGGAAGCCATTTTGGATTGGAGTCACGGGGAAAGTTCAAACTACATATACCAATTCCACAGTATTAGTTGAAACACAATGCGATAACGGAATTGAAGCGTGGGTGACTCTAGATCAAAAGCGAATGGCTATGCTAGAACAAGATCCCCAGTTTTTAGCATTTGCAGAATTGCATCTCCAACCCCCCGGGATTTCAATGGCCTCTTCAGTAGGTATTGATTAA

Protein Analysis

462

Amino Acids

52.27

Weight (kDa)

5.29

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 440 5.8e-61 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 2 cut(s) 272, 839
AclWI GGATC 1 cut(s) 1304
AcsI RAATTY 1 cut(s) 1140
AcuI CTGAAG 1 cut(s) 1356
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 4 cut(s) 218, 576, 722, 808
AfiI CCNNNNNNNGG 2 cut(s) 230, 410
AflII CTTAAG 1 cut(s) 847
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 9 cut(s) 160, 269, 419, 701, 813, 1205, 1238, 1262, 1362
AjnI CCWGG 1 cut(s) 509
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 8 cut(s) 53, 82, 328, 397, 472, 617, 928, 1131
AluI AGCT 8 cut(s) 53, 82, 328, 397, 472, 617, 928, 1131
Alw26I GTCTC 3 cut(s) 27, 460, 1019
AlwI GGATC 1 cut(s) 1304
Ama87I CYCGRG 4 cut(s) 728, 789, 1126, 1352
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 747, 1365
ApeKI GCWGC 4 cut(s) 53, 518, 569, 782
ApoI RAATTY 1 cut(s) 1140
ArsI GACNNNNNNTTYG 2 cut(s) 981, 1013
AspS9I GGNCC 1 cut(s) 748
AsuC2I CCSGG 5 cut(s) 122, 790, 791, 1353, 1354
AsuHPI GGTGA 3 cut(s) 853, 1049, 1282
AvaI CYCGRG 4 cut(s) 728, 789, 1126, 1352
BaeGI GKGCMC 1 cut(s) 729
BaeI ACNNNNGTAYC 2 cut(s) 1039, 1072
BanII GRGCYC 1 cut(s) 913
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 4 cut(s) 40, 505, 581, 769
BccI CCATC 8 cut(s) 47, 55, 95, 473, 616, 742, 1072, 1158
BciT130I CCWGG 1 cut(s) 511
BciVI GTATCC 1 cut(s) 1040
BclI TGATCA 1 cut(s) 955
BcnI CCSGG 5 cut(s) 122, 790, 791, 1353, 1354
BcoDI GTCTC 3 cut(s) 27, 460, 1019
BfaI CTAG 6 cut(s) 923, 1070, 1109, 1137, 1277, 1301
BfmI CTRYAG 1 cut(s) 1095
BfrI CTTAAG 1 cut(s) 847
BfuI GTATCC 1 cut(s) 1040
BisI GCNGC 4 cut(s) 54, 519, 570, 783
BlsI GCNGC 4 cut(s) 55, 520, 571, 784
BmcAI AGTACT 1 cut(s) 576
Bme1390I CCNGG 6 cut(s) 122, 511, 790, 791, 1353, 1354
BmeT110I CYCGRG 4 cut(s) 728, 789, 1126, 1352
BmgT120I GGNCC 1 cut(s) 748
BmiI GGNNCC 1 cut(s) 912
BmrFI CCNGG 6 cut(s) 122, 511, 790, 791, 1353, 1354
BmrI ACTGGG 1 cut(s) 1309
BmsI GCATC 1 cut(s) 1348
BmuI ACTGGG 1 cut(s) 1309
BpiI GAAGAC 1 cut(s) 148
BpuEI CTTGAG 3 cut(s) 308, 488, 549
BpuMI CCSGG 5 cut(s) 122, 790, 791, 1353, 1354
Bsa29I ATCGAT 3 cut(s) 93, 258, 477
BsaAI YACGTR 2 cut(s) 724, 740
BsaJI CCNNGG 4 cut(s) 510, 789, 1351, 1352
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 410
Bse1I ACTGG 2 cut(s) 760, 1315
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 1 cut(s) 511
BseCI ATCGAT 3 cut(s) 93, 258, 477
BseDI CCNNGG 4 cut(s) 510, 789, 1351, 1352
BseGI GGATG 2 cut(s) 550, 1169
BseLI CCNNNNNNNGG 2 cut(s) 230, 410
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 760, 1315
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 4 cut(s) 40, 505, 581, 769
BsgI GTGCAG 1 cut(s) 678
BshFI GGCC 2 cut(s) 749, 1367
BshVI ATCGAT 3 cut(s) 93, 258, 477
BsiHKCI CYCGRG 4 cut(s) 728, 789, 1126, 1352
BsiSI CCGG 5 cut(s) 122, 231, 790, 914, 1353
BslFI GGGAC 2 cut(s) 104, 263
BslI CCNNNNNNNGG 2 cut(s) 230, 410
BsmAI GTCTC 3 cut(s) 27, 460, 1019
BsmFI GGGAC 2 cut(s) 104, 263
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 2 cut(s) 749, 1367
BsoBI CYCGRG 4 cut(s) 728, 789, 1126, 1352
Bsp1286I GDGCHC 2 cut(s) 729, 913
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 4 cut(s) 94, 955, 1279, 1309
BspACI CCGC 2 cut(s) 272, 839
BspANI GGCC 2 cut(s) 749, 1367
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 3 cut(s) 93, 258, 477
BspEI TCCGGA 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 912
BspPI GGATC 1 cut(s) 1304
BspQI GCTCTTC 1 cut(s) 707
BspTI CTTAAG 1 cut(s) 847
BsrI ACTGG 2 cut(s) 760, 1315
BssECI CCNNGG 4 cut(s) 510, 789, 1351, 1352
BssMI GATC 4 cut(s) 94, 955, 1279, 1309
Bst2UI CCWGG 1 cut(s) 511
Bst4CI ACNGT 3 cut(s) 497, 1013, 1228
Bst6I CTCTTC 4 cut(s) 67, 707, 1077, 1375
BstAFI CTTAAG 1 cut(s) 847
BstBAI YACGTR 2 cut(s) 724, 740
BstDEI CTNAG 1 cut(s) 393
BstF5I GGATG 2 cut(s) 550, 1169
BstKTI GATC 4 cut(s) 97, 958, 1282, 1312
BstMAI GTCTC 3 cut(s) 27, 460, 1019
BstMBI GATC 4 cut(s) 94, 955, 1279, 1309
BstMWI GCNNNNNNNGC 3 cut(s) 692, 791, 836
BstNI CCWGG 1 cut(s) 511
BstSCI CCNGG 6 cut(s) 120, 509, 788, 789, 1351, 1352
BstSFI CTRYAG 1 cut(s) 1095
BstSLI GKGCMC 1 cut(s) 729
BstV1I GCAGC 4 cut(s) 40, 505, 581, 769
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 1 cut(s) 1309
BstYI RGATCY 1 cut(s) 1309
Bsu15I ATCGAT 3 cut(s) 93, 258, 477
BsuI GTATCC 1 cut(s) 1040
BsuRI GGCC 2 cut(s) 749, 1367
BsuTUI ATCGAT 3 cut(s) 93, 258, 477
BtsCI GGATG 2 cut(s) 550, 1169
BtsIMutI CAGTG 2 cut(s) 753, 1018
Cfr13I GGNCC 1 cut(s) 748
Cfr9I CCCGGG 2 cut(s) 789, 1352
ClaI ATCGAT 3 cut(s) 93, 258, 477
Csp6I GTAC 4 cut(s) 217, 575, 721, 807
CviAII CATG 2 cut(s) 310, 865
CviQI GTAC 4 cut(s) 217, 575, 721, 807
DdeI CTNAG 1 cut(s) 393
DpnI GATC 4 cut(s) 96, 957, 1281, 1311
DpnII GATC 4 cut(s) 94, 955, 1279, 1309
DraI TTTAAA 1 cut(s) 1032
Eam1104I CTCTTC 4 cut(s) 67, 707, 1077, 1375
EarI CTCTTC 4 cut(s) 67, 707, 1077, 1375
Eco24I GRGCYC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 1356
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 4 cut(s) 728, 789, 1126, 1352
EcoO109I RGGNCCY 1 cut(s) 748
EcoRII CCWGG 1 cut(s) 509
EcoT38I GRGCYC 1 cut(s) 913
FaeI CATG 2 cut(s) 313, 868
FalI AAGNNNNNCTT 2 cut(s) 808, 840
FaqI GGGAC 2 cut(s) 104, 263
FatI CATG 2 cut(s) 309, 864
FauI CCCGC 1 cut(s) 265
FbaI TGATCA 1 cut(s) 955
Fnu4HI GCNGC 4 cut(s) 54, 519, 570, 783
FokI GGATG 2 cut(s) 537, 1176
FriOI GRGCYC 1 cut(s) 913
Fsp4HI GCNGC 4 cut(s) 54, 519, 570, 783
FspBI CTAG 6 cut(s) 923, 1070, 1109, 1137, 1277, 1301
GluI GCNGC 4 cut(s) 54, 519, 570, 783
HaeIII GGCC 2 cut(s) 749, 1367
HapII CCGG 5 cut(s) 122, 231, 790, 914, 1353
Hin1II CATG 2 cut(s) 313, 868
HincII GTYRAC 1 cut(s) 979
HindII GTYRAC 1 cut(s) 979
HindIII AAGCTT 2 cut(s) 80, 326
HinfI GANTC 5 cut(s) 205, 556, 992, 1188, 1273
HpaI GTTAAC 1 cut(s) 979
HpaII CCGG 5 cut(s) 122, 231, 790, 914, 1353
HphI GGTGA 3 cut(s) 853, 1049, 1282
Hpy166II GTNNAC 2 cut(s) 844, 979
Hpy188I TCNGA 4 cut(s) 296, 394, 555, 621
Hpy188III TCNNGA 8 cut(s) 30, 231, 536, 701, 766, 855, 1126, 1277
Hpy8I GTNNAC 2 cut(s) 844, 979
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 2 cut(s) 228, 701
HpyCH4III ACNGT 3 cut(s) 497, 1013, 1228
HpyCH4IV ACGT 3 cut(s) 723, 739, 875
HpyCH4V TGCA 3 cut(s) 695, 1331, 1339
HpyF10VI GCNNNNNNNGC 3 cut(s) 692, 791, 836
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 3 cut(s) 723, 739, 875
Hsp92II CATG 2 cut(s) 313, 868
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 955
KspAI GTTAAC 1 cut(s) 979
Kzo9I GATC 4 cut(s) 94, 955, 1279, 1309
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 1 cut(s) 916
Lsp1109I GCAGC 4 cut(s) 40, 505, 581, 769
LweI GCATC 1 cut(s) 1348
MaeI CTAG 6 cut(s) 923, 1070, 1109, 1137, 1277, 1301
MaeII ACGT 3 cut(s) 723, 739, 875
MaeIII GTNAC 8 cut(s) 305, 464, 536, 655, 852, 968, 1189, 1270
MalI GATC 4 cut(s) 96, 957, 1281, 1311
MboI GATC 4 cut(s) 94, 955, 1279, 1309
MboII GAAGA 8 cut(s) 54, 124, 148, 155, 421, 724, 1094, 1362
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 1 cut(s) 1309
MhlI GDGCHC 2 cut(s) 729, 913
MluCI AATT 8 cut(s) 9, 301, 447, 931, 1140, 1219, 1257, 1334
MlyI GAGTC 3 cut(s) 199, 1197, 1267
MmeI TCCRAC 2 cut(s) 858, 1369
MnlI CCTC 9 cut(s) 7, 70, 84, 177, 283, 761, 1046, 1078, 1378
MroI TCCGGA 1 cut(s) 230
MseI TTAA 7 cut(s) 176, 185, 675, 848, 978, 1031, 1387
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 1 cut(s) 847
MspI CCGG 5 cut(s) 122, 231, 790, 914, 1353
MspR9I CCNGG 6 cut(s) 122, 511, 790, 791, 1353, 1354
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 1 cut(s) 511
MwoI GCNNNNNNNGC 3 cut(s) 692, 791, 836
NciI CCSGG 5 cut(s) 122, 790, 791, 1353, 1354
NdeII GATC 4 cut(s) 94, 955, 1279, 1309
NlaIII CATG 2 cut(s) 313, 868
NlaIV GGNNCC 1 cut(s) 912
NmuCI GTSAC 6 cut(s) 464, 536, 852, 968, 1189, 1270
PaeR7I CTCGAG 1 cut(s) 1126
PciSI GCTCTTC 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PctI GAATGC 1 cut(s) 917
PfeI GAWTC 2 cut(s) 556, 992
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 4 cut(s) 55, 520, 571, 784
PleI GAGTC 3 cut(s) 199, 1196, 1267
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 3 cut(s) 199, 1196, 1267
Ppu21I YACGTR 2 cut(s) 724, 740
Psp6I CCWGG 1 cut(s) 509
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 1 cut(s) 509
PspN4I GGNNCC 1 cut(s) 912
PspPI GGNCC 1 cut(s) 748
PsuI RGATCY 1 cut(s) 1309
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 4 cut(s) 218, 576, 722, 808
RsaNI GTAC 4 cut(s) 217, 575, 721, 807
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 7 cut(s) 176, 185, 675, 848, 978, 1031, 1387
SatI GCNGC 4 cut(s) 54, 519, 570, 783
Sau3AI GATC 4 cut(s) 94, 955, 1279, 1309
Sau96I GGNCC 1 cut(s) 748
ScaI AGTACT 1 cut(s) 576
SchI GAGTC 3 cut(s) 199, 1197, 1267
ScrFI CCNGG 6 cut(s) 122, 511, 790, 791, 1353, 1354
SduI GDGCHC 2 cut(s) 729, 913
SfaNI GCATC 1 cut(s) 1348
SfcI CTRYAG 1 cut(s) 1095
Sfr274I CTCGAG 1 cut(s) 1126
SlaI CTCGAG 1 cut(s) 1126
SmaI CCCGGG 2 cut(s) 791, 1354
SmlI CTYRAG 5 cut(s) 287, 503, 564, 847, 1126
SmoI CTYRAG 5 cut(s) 287, 503, 564, 847, 1126
Sse9I AATT 8 cut(s) 9, 301, 447, 931, 1140, 1219, 1257, 1334
SsiI CCGC 2 cut(s) 272, 839
SspMI CTAG 6 cut(s) 923, 1070, 1109, 1137, 1277, 1301
StyD4I CCNGG 6 cut(s) 120, 509, 788, 789, 1351, 1352
TaaI ACNGT 3 cut(s) 497, 1013, 1228
TaiI ACGT 3 cut(s) 726, 742, 878
TaqI TCGA 7 cut(s) 93, 169, 258, 366, 432, 477, 1127
TasI AATT 8 cut(s) 9, 301, 447, 931, 1140, 1219, 1257, 1334
TatI WGTACW 2 cut(s) 216, 574
TfiI GAWTC 2 cut(s) 556, 992
Tru1I TTAA 7 cut(s) 176, 185, 675, 848, 978, 1031, 1387
Tru9I TTAA 7 cut(s) 176, 185, 675, 848, 978, 1031, 1387
TscAI CASTG 2 cut(s) 760, 1018
TseFI GTSAC 6 cut(s) 464, 536, 852, 968, 1189, 1270
TseI GCWGC 4 cut(s) 53, 518, 569, 782
Tsp45I GTSAC 6 cut(s) 464, 536, 852, 968, 1189, 1270
TspDTI ATGAA 5 cut(s) 539, 720, 875, 881, 1110
TspGWI ACGGA 3 cut(s) 194, 1065, 1269
TspMI CCCGGG 2 cut(s) 789, 1352
TspRI CASTG 2 cut(s) 760, 1018
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 1 cut(s) 847
XapI RAATTY 1 cut(s) 1140
XbaI TCTAGA 1 cut(s) 1276
XcmI CCANNNNNNNNNTGG 2 cut(s) 339, 1157
XhoI CTCGAG 1 cut(s) 1126
XmaI CCCGGG 2 cut(s) 789, 1352
XspI CTAG 6 cut(s) 923, 1070, 1109, 1137, 1277, 1301
ZrmI AGTACT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.