RchiOBHm_Chr1g0362351

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006417
Physical Location & Seq
Forward (+)
54009418 .. 54011851
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58718

Sequence Viewer

Length: 1383 bp
ATGGAGAAAATTGAGGTCAGTATCATTTCCAGAGACACCATCAAACCATCAGCTGCTTCCTCTTCACTACTTCACCCTCACAAGCTTTCCATCATCGATCAAATCACTCCCACAATGTATGTCCCGGTTATATTCTTCTACCCCATTACTGATCCCGTCTTCAATCTTCCTCGAACCTTAACCGACTTAAAAAACACTCTTTCCCAGACTCTCACTTTGTACTATCCACTATCCGGGAGGGTCAAAAACAACTTATACATTGATGATTTTGAAGCGGGTGTCCCCTACCTTGAGGTCAGAGTAAATTGTAACATGATTGATTTTCTAAAGCTTCCAAAAACCGAGTGGCTAAATGAGTTTGTTCCAATCCAACCATATCGCAAGGAAACAATATCTGAGCTTCTCCCCTTGCTTGGAGTTCAAGTCAACATCTTCGACTACGGAATAGCAATTGGTGTCTCCCTTTGTCACAAGCTCATCGATGGCGAAACAGCAAACTGTTTTCTCAAGTCCTGGGTTGCTGCTTTTCGTGGGTGTCGTGACAAAATCATTCATCCGAATCTCTCTCAAGCAGCAGTACTTTTCCCACCGAGAGATGACTTGCCTGAAAAGTATGTAGCTCTGATGGAAAGGTGGTGCTTTTGCGAAAGAAATGTTGTTACAAGGAGATTTGTATTTAATGCGAAAGCCATATCTGCGCTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCGAGCCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCTCGGGCATTATCATCAGGTACTTCAACAAGATTTTCTTTAGCACTCCAAGCGGTGAACTTAAGGTCACGACTGAACATGAAAACGACGTTGGACAATGCCATTGGAAATATCTTTTTGTGGGCTCCGTCATTCCTAGAGCTAAATTATACAACACCAGAGAGTAGTGATCATAAGTTGTGTGACTTGGTTAACTTGCTCAAAGAATCTGTCAAAGAATATAACAGTGATTACTTGGAGACTTTAAAGGGTGAAAAGGGATACGGAGGCATGTGTGATTGGCTAGATTTGATGGAAGAGGGGAGTTCTATAGAACCAGCACTAGAGATTTATTCATTCTCGAGCTGGACTAGAATGTTTGACCAAATTGATTTTGGATGGGGGAAGCCATTTTGGATTGGAGTCACGGGGAAAGTTCAAACTACATATACCAATTCCACAGTATTAGTTGAAACACAATGCGACAACGGAATTGAAGCGTGGGTGACTCTAGATCAAAAGCGAATGGCTATGCTAGAACAAGATCCCCAGTTTTTGGCATTTGCATCTCCAACCCCCGGGATTTCAATGGCCTCTTCCGTAGGTATTGATTAA

Protein Analysis

460

Amino Acids

51.98

Weight (kDa)

5.29

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 441 8.9e-60 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1324
AciI CCGC 2 cut(s) 275, 842
AclWI GGATC 2 cut(s) 146, 1307
AcvI CACGTG 1 cut(s) 743
AfaI GTAC 4 cut(s) 221, 579, 725, 811
AfiI CCNNNNNNNGG 4 cut(s) 233, 413, 1324, 1346
AflII CTTAAG 1 cut(s) 850
AflIII ACRYGT 1 cut(s) 742
AgsI TTSAA 9 cut(s) 163, 272, 422, 704, 816, 1208, 1241, 1265, 1356
AjnI CCWGG 1 cut(s) 512
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
AluBI AGCT 8 cut(s) 53, 85, 331, 400, 475, 620, 931, 1134
AluI AGCT 8 cut(s) 53, 85, 331, 400, 475, 620, 931, 1134
Alw26I GTCTC 3 cut(s) 27, 463, 1022
AlwI GGATC 2 cut(s) 146, 1307
Ama87I CYCGRG 4 cut(s) 731, 792, 1129, 1346
AoxI GGCC 2 cut(s) 750, 1359
ApeKI GCWGC 4 cut(s) 53, 521, 572, 785
ArsI GACNNNNNNTTYG 4 cut(s) 199, 231, 984, 1016
AspLEI GCGC 1 cut(s) 700
AspS9I GGNCC 1 cut(s) 751
AsuC2I CCSGG 4 cut(s) 125, 235, 1347, 1348
AsuHPI GGTGA 4 cut(s) 65, 856, 1052, 1285
AvaI CYCGRG 4 cut(s) 731, 792, 1129, 1346
BaeGI GKGCMC 1 cut(s) 732
BanII GRGCYC 1 cut(s) 916
BarI GAAGNNNNNNTAC 2 cut(s) 707, 739
BbrPI CACGTG 1 cut(s) 743
BbsI GAAGAC 1 cut(s) 151
BbvI GCAGC 4 cut(s) 40, 508, 584, 772
BccI CCATC 8 cut(s) 47, 55, 98, 476, 619, 745, 1075, 1161
BciT130I CCWGG 1 cut(s) 514
BciVI GTATCC 1 cut(s) 1043
BclI TGATCA 1 cut(s) 958
BcnI CCSGG 4 cut(s) 125, 235, 1347, 1348
BcoDI GTCTC 3 cut(s) 27, 463, 1022
BfaI CTAG 6 cut(s) 926, 1073, 1112, 1140, 1280, 1304
BfmI CTRYAG 1 cut(s) 1098
BfrI CTTAAG 1 cut(s) 850
BfuI GTATCC 1 cut(s) 1043
BisI GCNGC 4 cut(s) 54, 522, 573, 786
BlsI GCNGC 4 cut(s) 55, 523, 574, 787
BmcAI AGTACT 1 cut(s) 579
Bme1390I CCNGG 5 cut(s) 125, 235, 514, 1347, 1348
BmeT110I CYCGRG 4 cut(s) 731, 792, 1129, 1346
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 1 cut(s) 915
BmrFI CCNGG 5 cut(s) 125, 235, 514, 1347, 1348
BmrI ACTGGG 1 cut(s) 1312
BmsI GCATC 1 cut(s) 1343
BmuI ACTGGG 1 cut(s) 1312
BpiI GAAGAC 1 cut(s) 151
BpuEI CTTGAG 3 cut(s) 311, 491, 552
BpuMI CCSGG 4 cut(s) 125, 235, 1347, 1348
Bsa29I ATCGAT 2 cut(s) 96, 480
BsaAI YACGTR 2 cut(s) 727, 743
BsaJI CCNNGG 3 cut(s) 513, 1345, 1346
BsaXI ACNNNNNCTCC 2 cut(s) 408, 438
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 413, 1324, 1346
Bse1I ACTGG 2 cut(s) 763, 1318
BseBI CCWGG 1 cut(s) 514
BseCI ATCGAT 2 cut(s) 96, 480
BseDI CCNNGG 3 cut(s) 513, 1345, 1346
BseGI GGATG 2 cut(s) 553, 1172
BseLI CCNNNNNNNGG 4 cut(s) 233, 413, 1324, 1346
BseMII CTCAG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 763, 1318
BseSI GKGCMC 1 cut(s) 732
BseXI GCAGC 4 cut(s) 40, 508, 584, 772
BshFI GGCC 2 cut(s) 752, 1361
BshVI ATCGAT 2 cut(s) 96, 480
BsiHKCI CYCGRG 4 cut(s) 731, 792, 1129, 1346
BsiSI CCGG 3 cut(s) 125, 234, 1347
BslFI GGGAC 2 cut(s) 107, 266
BslI CCNNNNNNNGG 4 cut(s) 233, 413, 1324, 1346
BsmAI GTCTC 3 cut(s) 27, 463, 1022
BsmFI GGGAC 2 cut(s) 107, 266
BsnI GGCC 2 cut(s) 752, 1361
BsoBI CYCGRG 4 cut(s) 731, 792, 1129, 1346
Bsp1286I GDGCHC 2 cut(s) 732, 916
Bsp143I GATC 5 cut(s) 97, 151, 958, 1282, 1312
BspACI CCGC 2 cut(s) 275, 842
BspANI GGCC 2 cut(s) 752, 1361
BspCNI CTCAG 1 cut(s) 388
BspDI ATCGAT 2 cut(s) 96, 480
BspLI GGNNCC 1 cut(s) 915
BspPI GGATC 2 cut(s) 146, 1307
BspQI GCTCTTC 1 cut(s) 710
BspTI CTTAAG 1 cut(s) 850
BsrI ACTGG 2 cut(s) 763, 1318
BssECI CCNNGG 3 cut(s) 513, 1345, 1346
BssMI GATC 5 cut(s) 97, 151, 958, 1282, 1312
Bst2UI CCWGG 1 cut(s) 514
Bst4CI ACNGT 3 cut(s) 500, 1016, 1231
Bst6I CTCTTC 4 cut(s) 67, 710, 1080, 1369
BstAFI CTTAAG 1 cut(s) 850
BstBAI YACGTR 2 cut(s) 727, 743
BstDEI CTNAG 1 cut(s) 396
BstF5I GGATG 2 cut(s) 553, 1172
BstHHI GCGC 1 cut(s) 700
BstKTI GATC 5 cut(s) 100, 154, 961, 1285, 1315
BstMAI GTCTC 3 cut(s) 27, 463, 1022
BstMBI GATC 5 cut(s) 97, 151, 958, 1282, 1312
BstMWI GCNNNNNNNGC 3 cut(s) 695, 794, 839
BstNI CCWGG 1 cut(s) 514
BstNSI RCATGY 1 cut(s) 1063
BstSCI CCNGG 5 cut(s) 123, 233, 512, 1345, 1346
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 1 cut(s) 732
BstV1I GCAGC 4 cut(s) 40, 508, 584, 772
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 1312
BstYI RGATCY 1 cut(s) 1312
Bsu15I ATCGAT 2 cut(s) 96, 480
BsuI GTATCC 1 cut(s) 1043
BsuRI GGCC 2 cut(s) 752, 1361
BsuTUI ATCGAT 2 cut(s) 96, 480
BtsCI GGATG 2 cut(s) 553, 1172
BtsIMutI CAGTG 2 cut(s) 756, 1021
CfoI GCGC 1 cut(s) 700
Cfr13I GGNCC 1 cut(s) 751
Cfr9I CCCGGG 1 cut(s) 1346
ClaI ATCGAT 2 cut(s) 96, 480
Csp6I GTAC 4 cut(s) 220, 578, 724, 810
CviAII CATG 3 cut(s) 313, 868, 1060
CviQI GTAC 4 cut(s) 220, 578, 724, 810
DdeI CTNAG 1 cut(s) 396
DpnI GATC 5 cut(s) 99, 153, 960, 1284, 1314
DpnII GATC 5 cut(s) 97, 151, 958, 1282, 1312
DraI TTTAAA 1 cut(s) 1035
Eam1104I CTCTTC 4 cut(s) 67, 710, 1080, 1369
EarI CTCTTC 4 cut(s) 67, 710, 1080, 1369
Eco24I GRGCYC 1 cut(s) 916
Eco72I CACGTG 1 cut(s) 743
Eco88I CYCGRG 4 cut(s) 731, 792, 1129, 1346
EcoO109I RGGNCCY 1 cut(s) 751
EcoRII CCWGG 1 cut(s) 512
EcoT38I GRGCYC 1 cut(s) 916
FaeI CATG 3 cut(s) 316, 871, 1063
FalI AAGNNNNNCTT 2 cut(s) 811, 843
FaqI GGGAC 2 cut(s) 107, 266
FatI CATG 3 cut(s) 312, 867, 1059
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 958
Fnu4HI GCNGC 4 cut(s) 54, 522, 573, 786
FokI GGATG 2 cut(s) 540, 1179
FriOI GRGCYC 1 cut(s) 916
Fsp4HI GCNGC 4 cut(s) 54, 522, 573, 786
FspBI CTAG 6 cut(s) 926, 1073, 1112, 1140, 1280, 1304
GlaI GCGC 1 cut(s) 699
GluI GCNGC 4 cut(s) 54, 522, 573, 786
HaeIII GGCC 2 cut(s) 752, 1361
HapII CCGG 3 cut(s) 125, 234, 1347
HhaI GCGC 1 cut(s) 700
Hin1II CATG 3 cut(s) 316, 871, 1063
Hin6I GCGC 1 cut(s) 698
HinP1I GCGC 1 cut(s) 698
HincII GTYRAC 2 cut(s) 427, 982
HindII GTYRAC 2 cut(s) 427, 982
HindIII AAGCTT 2 cut(s) 83, 329
HinfI GANTC 5 cut(s) 208, 559, 995, 1191, 1276
HpaI GTTAAC 1 cut(s) 982
HpaII CCGG 3 cut(s) 125, 234, 1347
HphI GGTGA 4 cut(s) 65, 856, 1052, 1285
Hpy166II GTNNAC 3 cut(s) 427, 847, 982
Hpy188I TCNGA 4 cut(s) 299, 397, 558, 624
Hpy188III TCNNGA 7 cut(s) 30, 539, 704, 769, 858, 1129, 1280
Hpy8I GTNNAC 3 cut(s) 427, 847, 982
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 1 cut(s) 704
HpyCH4III ACNGT 3 cut(s) 500, 1016, 1231
HpyCH4IV ACGT 3 cut(s) 726, 742, 878
HpyCH4V TGCA 1 cut(s) 1334
HpyF10VI GCNNNNNNNGC 3 cut(s) 695, 794, 839
HpyF3I CTNAG 1 cut(s) 396
HpySE526I ACGT 3 cut(s) 726, 742, 878
Hsp92II CATG 3 cut(s) 316, 871, 1063
HspAI GCGC 1 cut(s) 698
Ksp22I TGATCA 1 cut(s) 958
KspAI GTTAAC 1 cut(s) 982
Kzo9I GATC 5 cut(s) 97, 151, 958, 1282, 1312
LguI GCTCTTC 1 cut(s) 710
LmnI GCTCC 1 cut(s) 919
Lsp1109I GCAGC 4 cut(s) 40, 508, 584, 772
LweI GCATC 1 cut(s) 1343
MaeI CTAG 6 cut(s) 926, 1073, 1112, 1140, 1280, 1304
MaeII ACGT 3 cut(s) 726, 742, 878
MaeIII GTNAC 8 cut(s) 308, 467, 539, 658, 855, 971, 1192, 1273
MalI GATC 5 cut(s) 99, 153, 960, 1284, 1314
MboI GATC 5 cut(s) 97, 151, 958, 1282, 1312
MboII GAAGA 8 cut(s) 54, 127, 151, 158, 424, 727, 1097, 1356
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 1312
MhlI GDGCHC 2 cut(s) 732, 916
MluCI AATT 7 cut(s) 9, 304, 450, 934, 1155, 1222, 1260
MlyI GAGTC 3 cut(s) 202, 1200, 1270
MmeI TCCRAC 3 cut(s) 394, 861, 1364
MseI TTAA 7 cut(s) 179, 188, 678, 851, 981, 1034, 1381
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 3 cut(s) 125, 234, 1347
MspR9I CCNGG 5 cut(s) 125, 235, 514, 1347, 1348
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 1 cut(s) 514
MwoI GCNNNNNNNGC 3 cut(s) 695, 794, 839
NciI CCSGG 4 cut(s) 125, 235, 1347, 1348
NdeII GATC 5 cut(s) 97, 151, 958, 1282, 1312
NlaIII CATG 3 cut(s) 316, 871, 1063
NlaIV GGNNCC 1 cut(s) 915
NmuCI GTSAC 6 cut(s) 467, 539, 855, 971, 1192, 1273
NspI RCATGY 1 cut(s) 1063
PaeR7I CTCGAG 1 cut(s) 1129
PciSI GCTCTTC 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 535
PfeI GAWTC 2 cut(s) 559, 995
PflMI CCANNNNNTGG 1 cut(s) 1324
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 4 cut(s) 55, 523, 574, 787
PleI GAGTC 3 cut(s) 202, 1199, 1270
PmaCI CACGTG 1 cut(s) 743
PmlI CACGTG 1 cut(s) 743
PpsI GAGTC 3 cut(s) 202, 1199, 1270
Ppu21I YACGTR 2 cut(s) 727, 743
Psp6I CCWGG 1 cut(s) 512
PspCI CACGTG 1 cut(s) 743
PspGI CCWGG 1 cut(s) 512
PspN4I GGNNCC 1 cut(s) 915
PspPI GGNCC 1 cut(s) 751
PsuI RGATCY 1 cut(s) 1312
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 4 cut(s) 221, 579, 725, 811
RsaNI GTAC 4 cut(s) 220, 578, 724, 810
SapI GCTCTTC 1 cut(s) 710
SaqAI TTAA 7 cut(s) 179, 188, 678, 851, 981, 1034, 1381
SatI GCNGC 4 cut(s) 54, 522, 573, 786
Sau3AI GATC 5 cut(s) 97, 151, 958, 1282, 1312
Sau96I GGNCC 1 cut(s) 751
ScaI AGTACT 1 cut(s) 579
SchI GAGTC 3 cut(s) 202, 1200, 1270
ScrFI CCNGG 5 cut(s) 125, 235, 514, 1347, 1348
SduI GDGCHC 2 cut(s) 732, 916
SfaNI GCATC 1 cut(s) 1343
SfcI CTRYAG 1 cut(s) 1098
Sfr274I CTCGAG 1 cut(s) 1129
SlaI CTCGAG 1 cut(s) 1129
SmaI CCCGGG 1 cut(s) 1348
SmlI CTYRAG 5 cut(s) 290, 506, 567, 850, 1129
SmoI CTYRAG 5 cut(s) 290, 506, 567, 850, 1129
Sse9I AATT 7 cut(s) 9, 304, 450, 934, 1155, 1222, 1260
SsiI CCGC 2 cut(s) 275, 842
SspMI CTAG 6 cut(s) 926, 1073, 1112, 1140, 1280, 1304
StyD4I CCNGG 5 cut(s) 123, 233, 512, 1345, 1346
TaaI ACNGT 3 cut(s) 500, 1016, 1231
TaiI ACGT 3 cut(s) 729, 745, 881
TaqI TCGA 5 cut(s) 96, 172, 435, 480, 1130
TasI AATT 7 cut(s) 9, 304, 450, 934, 1155, 1222, 1260
TatI WGTACW 2 cut(s) 219, 577
TfiI GAWTC 2 cut(s) 559, 995
Tru1I TTAA 7 cut(s) 179, 188, 678, 851, 981, 1034, 1381
Tru9I TTAA 7 cut(s) 179, 188, 678, 851, 981, 1034, 1381
TscAI CASTG 2 cut(s) 763, 1021
TseFI GTSAC 6 cut(s) 467, 539, 855, 971, 1192, 1273
TseI GCWGC 4 cut(s) 53, 521, 572, 785
Tsp45I GTSAC 6 cut(s) 467, 539, 855, 971, 1192, 1273
TspDTI ATGAA 4 cut(s) 542, 723, 884, 1113
TspGWI ACGGA 5 cut(s) 456, 906, 1068, 1272, 1357
TspMI CCCGGG 1 cut(s) 1346
TspRI CASTG 2 cut(s) 763, 1021
Van91I CCANNNNNTGG 1 cut(s) 1324
Vha464I CTTAAG 1 cut(s) 850
XbaI TCTAGA 1 cut(s) 1279
XceI RCATGY 1 cut(s) 1063
XcmI CCANNNNNNNNNTGG 2 cut(s) 342, 1160
XhoI CTCGAG 1 cut(s) 1129
XmaI CCCGGG 1 cut(s) 1346
XspI CTAG 6 cut(s) 926, 1073, 1112, 1140, 1280, 1304
ZrmI AGTACT 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.