Rh2DG152500

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006417
Physical Location & Seq
Forward (+)
12813916 .. 12814734
819 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG152500.1

Sequence Viewer

Length: 819 bp
ATGGAGAAAATTAAGGTCAGTATTATTTCCAGAGACACCATTAAACCATCAGCTGCTTCCTCTTCACTACACCCTTACAAGCTTTCCATCATCGATCAGTTCACTCCCACAATGTATTTCCCAGTTATATTCTTCTACCCCATTACTGACCGTGTCTTCAATCTTCCTCAAACCTTAACCGACTTGAAAAACACTGTTTCCCAGGCTCTCACTTTGTACTATCCACTCTCCGGAAGGATAAAAAACAACCTATACATTGATGATTTCGAAGCAGGCATCCCCTACCTTGAGGCCCGAGTGAATTTTCACATGATTGATTTTCTAAGGCTTCCGAAAATCGAGTGGCTAAATGAGTTTGTTCCAATAGCTCCATATCGCAAGGAAACAATATCTGAGTTTCTTCCCTTGCTTGGAATTCAAGTAAACATTTTCGACTCTGGAATAGCAATCGGTGTCTCTTTCTCTCACAAGATCAACGATGGCAAAACGGCAAGCTGTTTTCTCAAGTCCTGGGTTGCTATTTTTCGTGGGTATCGTAACAAAATCATACATCCTAATCTCTCTCAAGCTGCATTACTTTTTCCATCGAGGGATGACTTGCCTGAAAAGTATGTAGCTATGATGGAAAGGTTGTGGTTTGGCGAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCGCACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCTCGGGCATTATCATCAGGTAAATCAGTTTAG

Protein Analysis

272

Amino Acids

31.05

Weight (kDa)

9.48

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 268 1.9e-41 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 1 cut(s) 693
AcsI RAATTY 2 cut(s) 301, 414
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 2 cut(s) 218, 722
AfiI CCNNNNNNNGG 2 cut(s) 230, 410
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 4 cut(s) 160, 187, 419, 701
AjnI CCWGG 2 cut(s) 201, 509
AluBI AGCT 6 cut(s) 53, 82, 368, 495, 569, 617
AluI AGCT 6 cut(s) 53, 82, 368, 495, 569, 617
Alw26I GTCTC 2 cut(s) 27, 460
Ama87I CYCGRG 2 cut(s) 294, 789
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 2 cut(s) 291, 747
ApeKI GCWGC 3 cut(s) 53, 569, 782
ApoI RAATTY 2 cut(s) 301, 414
AspS9I GGNCC 2 cut(s) 292, 748
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 2 cut(s) 294, 789
BaeGI GKGCMC 1 cut(s) 729
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 40, 556, 769
BccI CCATC 6 cut(s) 55, 95, 473, 592, 616, 742
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 2 cut(s) 203, 511
BcoDI GTCTC 2 cut(s) 27, 460
BisI GCNGC 3 cut(s) 54, 570, 783
BlsI GCNGC 3 cut(s) 55, 571, 784
Bme1390I CCNGG 2 cut(s) 203, 511
BmeT110I CYCGRG 2 cut(s) 294, 789
BmgT120I GGNCC 2 cut(s) 292, 748
BmrFI CCNGG 2 cut(s) 203, 511
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 2 cut(s) 285, 667
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 308, 488, 549
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 2 cut(s) 724, 740
BsaJI CCNNGG 2 cut(s) 201, 510
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 410
Bse1I ACTGG 2 cut(s) 122, 760
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 2 cut(s) 203, 511
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 201, 510
BseGI GGATG 3 cut(s) 276, 550, 598
BseLI CCNNNNNNNGG 2 cut(s) 230, 410
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 122, 760
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 3 cut(s) 40, 556, 769
BshFI GGCC 2 cut(s) 293, 749
BshVI ATCGAT 1 cut(s) 93
BsiHKCI CYCGRG 2 cut(s) 294, 789
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 230, 410
BsmAI GTCTC 2 cut(s) 27, 460
BsnI GGCC 2 cut(s) 293, 749
BsoBI CYCGRG 2 cut(s) 294, 789
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 729
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 2 cut(s) 94, 471
BspACI CCGC 1 cut(s) 693
BspANI GGCC 2 cut(s) 293, 749
BspCNI CTCAG 1 cut(s) 385
BspDI ATCGAT 1 cut(s) 93
BspEI TCCGGA 1 cut(s) 230
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 122, 760
BssECI CCNNGG 2 cut(s) 201, 510
BssMI GATC 2 cut(s) 94, 471
Bst2UI CCWGG 2 cut(s) 203, 511
Bst4CI ACNGT 2 cut(s) 152, 196
Bst6I CTCTTC 2 cut(s) 67, 707
BstBAI YACGTR 2 cut(s) 724, 740
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 2 cut(s) 274, 493
BstDEI CTNAG 2 cut(s) 323, 393
BstF5I GGATG 3 cut(s) 276, 550, 598
BstKTI GATC 2 cut(s) 97, 474
BstMAI GTCTC 2 cut(s) 27, 460
BstMBI GATC 2 cut(s) 94, 471
BstMWI GCNNNNNNNGC 2 cut(s) 692, 791
BstNI CCWGG 2 cut(s) 203, 511
BstSCI CCNGG 2 cut(s) 201, 509
BstSLI GKGCMC 1 cut(s) 729
BstV1I GCAGC 3 cut(s) 40, 556, 769
BstV2I GAAGAC 1 cut(s) 148
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 2 cut(s) 293, 749
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 3 cut(s) 276, 550, 598
BtsIMutI CAGTG 2 cut(s) 192, 753
Cac8I GCNNGC 2 cut(s) 274, 493
Cfr13I GGNCC 2 cut(s) 292, 748
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 2 cut(s) 217, 721
CviAII CATG 1 cut(s) 310
CviQI GTAC 2 cut(s) 217, 721
DdeI CTNAG 2 cut(s) 323, 393
DpnI GATC 2 cut(s) 96, 473
DpnII GATC 2 cut(s) 94, 471
Eam1104I CTCTTC 2 cut(s) 67, 707
EarI CTCTTC 2 cut(s) 67, 707
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 2 cut(s) 294, 789
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 2 cut(s) 201, 509
FaeI CATG 1 cut(s) 313
FaiI YATR 8 cut(s) 128, 253, 311, 373, 548, 612, 620, 689
FatI CATG 1 cut(s) 309
Fnu4HI GCNGC 3 cut(s) 54, 570, 783
FokI GGATG 3 cut(s) 263, 537, 605
Fsp4HI GCNGC 3 cut(s) 54, 570, 783
GluI GCNGC 3 cut(s) 54, 570, 783
HaeIII GGCC 2 cut(s) 293, 749
HapII CCGG 1 cut(s) 231
Hin1II CATG 1 cut(s) 313
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 1 cut(s) 434
HpaII CCGG 1 cut(s) 231
Hpy166II GTNNAC 2 cut(s) 102, 424
Hpy188I TCNGA 2 cut(s) 333, 394
Hpy188III TCNNGA 5 cut(s) 30, 231, 438, 701, 766
Hpy8I GTNNAC 2 cut(s) 102, 424
HpyAV CCTTC 2 cut(s) 228, 701
HpyCH4III ACNGT 2 cut(s) 152, 196
HpyCH4IV ACGT 2 cut(s) 723, 739
HpyCH4V TGCA 1 cut(s) 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 791
HpyF3I CTNAG 2 cut(s) 323, 393
HpySE526I ACGT 2 cut(s) 723, 739
Hsp92II CATG 1 cut(s) 313
Kpn2I TCCGGA 1 cut(s) 230
Kzo9I GATC 2 cut(s) 94, 471
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 1 cut(s) 373
Lsp1109I GCAGC 3 cut(s) 40, 556, 769
LweI GCATC 2 cut(s) 285, 667
MaeII ACGT 2 cut(s) 723, 739
MaeIII GTNAC 2 cut(s) 536, 655
MalI GATC 2 cut(s) 96, 473
MboI GATC 2 cut(s) 94, 471
MboII GAAGA 6 cut(s) 54, 124, 148, 155, 392, 724
MhlI GDGCHC 1 cut(s) 729
MluCI AATT 3 cut(s) 9, 301, 414
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 5 cut(s) 70, 177, 283, 582, 761
MroI TCCGGA 1 cut(s) 230
MseI TTAA 3 cut(s) 12, 42, 176
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 2 cut(s) 203, 511
MvaI CCWGG 2 cut(s) 203, 511
MwoI GCNNNNNNNGC 2 cut(s) 692, 791
NdeII GATC 2 cut(s) 94, 471
NlaIII CATG 1 cut(s) 313
NspV TTCGAA 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 55, 571, 784
PleI GAGTC 1 cut(s) 428
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 1 cut(s) 428
Ppu21I YACGTR 2 cut(s) 724, 740
Psp6I CCWGG 2 cut(s) 201, 509
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 2 cut(s) 201, 509
PspPI GGNCC 2 cut(s) 292, 748
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 2 cut(s) 218, 722
RsaNI GTAC 2 cut(s) 217, 721
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 3 cut(s) 12, 42, 176
SatI GCNGC 3 cut(s) 54, 570, 783
Sau3AI GATC 2 cut(s) 94, 471
Sau96I GGNCC 2 cut(s) 292, 748
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 203, 511
SduI GDGCHC 1 cut(s) 729
SfaNI GCATC 2 cut(s) 285, 667
SfuI TTCGAA 1 cut(s) 267
SmlI CTYRAG 3 cut(s) 287, 503, 564
SmoI CTYRAG 3 cut(s) 287, 503, 564
Sse9I AATT 3 cut(s) 9, 301, 414
SsiI CCGC 1 cut(s) 693
StyD4I CCNGG 2 cut(s) 201, 509
TaaI ACNGT 2 cut(s) 152, 196
TaiI ACGT 2 cut(s) 726, 742
TaqI TCGA 5 cut(s) 93, 267, 339, 432, 587
TasI AATT 3 cut(s) 9, 301, 414
TatI WGTACW 1 cut(s) 216
Tru1I TTAA 3 cut(s) 12, 42, 176
Tru9I TTAA 3 cut(s) 12, 42, 176
TscAI CASTG 2 cut(s) 199, 760
TseI GCWGC 3 cut(s) 53, 569, 782
TspDTI ATGAA 1 cut(s) 720
TspRI CASTG 2 cut(s) 199, 760
Tth111I GACNNNGTC 1 cut(s) 152
XapI RAATTY 2 cut(s) 301, 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.