FvH4_7g18570

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006417
Physical Location & Seq
Forward (+)
15467224 .. 15471989
4766 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18570.t1

Sequence Viewer

Length: 1368 bp
ATGGAGAAAATTGAGGTCAGTATAATTTCCAAACACACCATCAAACCATCAGCTTCCACTCCACGACTTCAGCCTTACAAGCTTACCCTCCTGGACCAGCTCACTCCTGCAATGTATGTCCCCATGGTATTCTTCTACCCCATTACTGATCCTGTCTTCAATCTTCCTCAAACCCTAGCTGACTTAAGACAAGCCCTTTCCGAGACTCTCACTTTGTACTATCCACTCTCTGGAAGGGTAAAAAACAACCTATACATCGATGATTTTGAAGAGGGTGTCCCATACCTTGAGGCTCGAGTGAATTGTGACATAACTGATTTCCTAATGCTTCGGAAAATCGAGTGCCTAAATGAGTTTGTTCCAATAAAAACATTTAGTATGGAAGCAATATCTGATGAGCGTTACCCTTTGCTTGGAGTTCAAGTCAACATTTTCGACTCTGGAATAGCAATCGGTGTCTCCGGCTCTCACAAGCTCATTGATGGAAGAACGGCAGACTGTTTTCTCAAGTCCTGGGGTGCTGTTTTTCGAGGGTGTCGTGAAGATATCATACATCCTTGTCTCTCTGAAGCAGCATTGCTTTTCCCACCGAGAGATGACCTGCCTGAAAAGTATATAGCTTTGATGGAAGGGTTACGGTTTGCCGGAAAAAAAGTTTCTACGAGGAGATTCGTATTTGATGCGAAAACCATATCTGCAATTCAAGATGAAGCGAAGAGCGAGTCCGTGCCCAAGCCATCACGAGTTCAGGCCGTCACTGGTTTTCTCTGGAAACATCTAATCGTTGCTTCTCGGGCACTAACATCAGGTACTACTTCAACAAAACTTTCTATAGCAGCCCAGGCAGTGAACATAAGATCACGGATGAACATGGAGACGGTGTTGGATAATGCCACTGGAAACTTGATGTGGTGGGCACCGGCCATACTAGAGCTAAGTCATACAACACCAGAGATTAGTGATCTTAAGCTGTGTGACTTGGTTAACTTGCTCAATGGATCTGTCAAACAATGTAACGATGATTACTTTGAGACTTTCAAGGGTAAAGAGGGATATGAAAGCATGTGTGAGTATCTAGATTTTCAGAGGATTATGAGTTCTATAGAACCAACACCAGAGATTTATACATTCTCGAGCTGGACCAGTTTTTTCAACCAACTTGATTTTGGATGGGGGAGGTCATCTTGGATTGGAGTTACAGGAAAAATTGAATCTGCATATTGCAATTCCACAGTGTTGGTTCCAACACAATGCGATACTGGAATTGAAGCGTGGGTAAGTCTAGAAGAAGAGAAAATGGCTACGCTAAAACAAGATCCCCAGTTTCTAGCGGCAGCATCTCCAAACCCCCCAGTTTCAAGGTGTTGA

Protein Analysis

456

Amino Acids

51.03

Weight (kDa)

5.16

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 442 6e-66 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 609
AccB1I GGYRCC 1 cut(s) 916
AccB7I CCANNNNNTGG 1 cut(s) 230
AciI CCGC 1 cut(s) 1331
AclWI GGATC 3 cut(s) 143, 1006, 1310
AcoI YGGCCR 1 cut(s) 921
AcuI CTGAAG 2 cut(s) 53, 588
AfaI GTAC 2 cut(s) 218, 811
AfiI CCNNNNNNNGG 2 cut(s) 230, 413
AflII CTTAAG 2 cut(s) 184, 965
AjnI CCWGG 3 cut(s) 90, 512, 840
AloI GAACNNNNNNTCC 2 cut(s) 1081, 1113
AluBI AGCT 9 cut(s) 53, 82, 100, 179, 475, 620, 934, 970, 1137
AluI AGCT 9 cut(s) 53, 82, 100, 179, 475, 620, 934, 970, 1137
Alw26I GTCTC 5 cut(s) 197, 463, 566, 869, 1025
AlwI GGATC 3 cut(s) 143, 1006, 1310
Ama87I CYCGRG 3 cut(s) 294, 792, 1132
AoxI GGCC 2 cut(s) 750, 921
ApeKI GCWGC 3 cut(s) 572, 836, 1334
ArsI GACNNNNNNTTYG 4 cut(s) 196, 228, 707, 739
AspS9I GGNCC 2 cut(s) 94, 1140
AvaI CYCGRG 3 cut(s) 294, 792, 1132
AvaII GGWCC 2 cut(s) 94, 1140
BaeGI GKGCMC 3 cut(s) 732, 799, 919
BanI GGYRCC 1 cut(s) 916
BarI GAAGNNNNNNTAC 2 cut(s) 534, 566
BauI CACGAG 1 cut(s) 741
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 584, 848, 1346
BccI CCATC 6 cut(s) 47, 55, 476, 619, 745, 1164
BceAI ACGGC 2 cut(s) 507, 737
BciT130I CCWGG 3 cut(s) 92, 514, 842
BcoDI GTCTC 5 cut(s) 197, 463, 566, 869, 1025
BfaI CTAG 5 cut(s) 176, 929, 1076, 1283, 1328
BfmI CTRYAG 2 cut(s) 831, 1101
BfrI CTTAAG 2 cut(s) 184, 965
BfuAI ACCTGC 1 cut(s) 609
BisI GCNGC 4 cut(s) 573, 837, 1332, 1335
BlsI GCNGC 4 cut(s) 574, 838, 1333, 1336
Bme1390I CCNGG 3 cut(s) 92, 514, 842
Bme18I GGWCC 2 cut(s) 94, 1140
BmeT110I CYCGRG 3 cut(s) 294, 792, 1132
BmgT120I GGNCC 2 cut(s) 94, 1140
BmiI GGNNCC 2 cut(s) 918, 1242
BmrFI CCNGG 3 cut(s) 92, 514, 842
BmrI ACTGGG 2 cut(s) 1315, 1346
BmsI GCATC 2 cut(s) 670, 1346
BmuI ACTGGG 2 cut(s) 1315, 1346
BpiI GAAGAC 1 cut(s) 148
BpuEI CTTGAG 2 cut(s) 308, 491
Bsa29I ATCGAT 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 123, 513, 840
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 866, 896
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 413
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 6 cut(s) 763, 901, 1143, 1264, 1321, 1352
Bse3DI GCAATG 2 cut(s) 117, 575
BseBI CCWGG 3 cut(s) 92, 514, 842
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 123, 513, 840
BseGI GGATG 3 cut(s) 553, 870, 1175
BseLI CCNNNNNNNGG 2 cut(s) 230, 413
BseMI GCAATG 2 cut(s) 117, 575
BseNI ACTGG 6 cut(s) 763, 901, 1143, 1264, 1321, 1352
BseRI GAGGAG 1 cut(s) 679
BseSI GKGCMC 3 cut(s) 732, 799, 919
BseXI GCAGC 3 cut(s) 584, 848, 1346
BshFI GGCC 2 cut(s) 752, 923
BshNI GGYRCC 1 cut(s) 916
BshVI ATCGAT 1 cut(s) 258
BsiHKCI CYCGRG 3 cut(s) 294, 792, 1132
BsiSI CCGG 3 cut(s) 462, 645, 920
BslFI GGGAC 2 cut(s) 104, 263
BslI CCNNNNNNNGG 2 cut(s) 230, 413
BsmAI GTCTC 5 cut(s) 197, 463, 566, 869, 1025
BsmBI CGTCTC 1 cut(s) 869
BsmFI GGGAC 2 cut(s) 104, 263
BsnI GGCC 2 cut(s) 752, 923
BsoBI CYCGRG 3 cut(s) 294, 792, 1132
Bsp1286I GDGCHC 3 cut(s) 732, 799, 919
Bsp143I GATC 5 cut(s) 148, 857, 961, 998, 1315
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 1 cut(s) 1331
BspANI GGCC 2 cut(s) 752, 923
BspDI ATCGAT 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 918, 1242
BspMI ACCTGC 1 cut(s) 609
BspPI GGATC 3 cut(s) 143, 1006, 1310
BspQI GCTCTTC 1 cut(s) 710
BspT107I GGYRCC 1 cut(s) 916
BspTI CTTAAG 2 cut(s) 184, 965
BsrDI GCAATG 2 cut(s) 117, 575
BsrFI RCCGGY 1 cut(s) 919
BsrI ACTGG 6 cut(s) 763, 901, 1143, 1264, 1321, 1352
BssAI RCCGGY 1 cut(s) 919
BssECI CCNNGG 3 cut(s) 123, 513, 840
BssMI GATC 5 cut(s) 148, 857, 961, 998, 1315
BssSI CACGAG 1 cut(s) 741
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 741
Bst2UI CCWGG 3 cut(s) 92, 514, 842
Bst4CI ACNGT 4 cut(s) 500, 639, 880, 1234
Bst6I CTCTTC 3 cut(s) 264, 710, 1284
BstAFI CTTAAG 2 cut(s) 184, 965
BstDEI CTNAG 1 cut(s) 935
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 3 cut(s) 553, 870, 1175
BstKTI GATC 5 cut(s) 151, 860, 964, 1001, 1318
BstMAI GTCTC 5 cut(s) 197, 463, 566, 869, 1025
BstMBI GATC 5 cut(s) 148, 857, 961, 998, 1315
BstMWI GCNNNNNNNGC 3 cut(s) 79, 794, 842
BstNI CCWGG 3 cut(s) 92, 514, 842
BstNSI RCATGY 1 cut(s) 1066
BstSCI CCNGG 3 cut(s) 90, 512, 840
BstSFI CTRYAG 2 cut(s) 831, 1101
BstSLI GKGCMC 3 cut(s) 732, 799, 919
BstV1I GCAGC 3 cut(s) 584, 848, 1346
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 998, 1315
BstXI CCANNNNNNTGG 1 cut(s) 1237
BstYI RGATCY 2 cut(s) 998, 1315
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 2 cut(s) 752, 923
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 3 cut(s) 553, 870, 1175
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 4 cut(s) 756, 852, 894, 1239
BveI ACCTGC 1 cut(s) 609
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 2 cut(s) 94, 1140
ClaI ATCGAT 1 cut(s) 258
Csp6I GTAC 2 cut(s) 217, 810
CviAII CATG 3 cut(s) 124, 871, 1063
CviQI GTAC 2 cut(s) 217, 810
DdeI CTNAG 1 cut(s) 935
DpnI GATC 5 cut(s) 150, 859, 963, 1000, 1317
DpnII GATC 5 cut(s) 148, 857, 961, 998, 1315
EaeI YGGCCR 1 cut(s) 921
Eam1104I CTCTTC 3 cut(s) 264, 710, 1284
EarI CTCTTC 3 cut(s) 264, 710, 1284
Eco130I CCWWGG 1 cut(s) 123
Eco32I GATATC 1 cut(s) 547
Eco47I GGWCC 2 cut(s) 94, 1140
Eco57I CTGAAG 2 cut(s) 53, 588
Eco88I CYCGRG 3 cut(s) 294, 792, 1132
EcoRII CCWGG 3 cut(s) 90, 512, 840
EcoRV GATATC 1 cut(s) 547
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 869
FaeI CATG 3 cut(s) 127, 874, 1066
FaqI GGGAC 2 cut(s) 104, 263
FatI CATG 3 cut(s) 123, 870, 1062
Fnu4HI GCNGC 4 cut(s) 573, 837, 1332, 1335
FokI GGATG 3 cut(s) 540, 877, 1182
Fsp4HI GCNGC 4 cut(s) 573, 837, 1332, 1335
FspBI CTAG 5 cut(s) 176, 929, 1076, 1283, 1328
GluI GCNGC 4 cut(s) 573, 837, 1332, 1335
HaeIII GGCC 2 cut(s) 752, 923
HapII CCGG 3 cut(s) 462, 645, 920
Hin1II CATG 3 cut(s) 127, 874, 1066
HincII GTYRAC 2 cut(s) 427, 985
HindII GTYRAC 2 cut(s) 427, 985
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 5 cut(s) 205, 437, 669, 722, 1211
HpaI GTTAAC 1 cut(s) 985
HpaII CCGG 3 cut(s) 462, 645, 920
Hpy166II GTNNAC 3 cut(s) 427, 850, 985
Hpy188I TCNGA 5 cut(s) 202, 333, 394, 568, 1086
Hpy188III TCNNGA 9 cut(s) 231, 441, 539, 704, 741, 769, 1076, 1132, 1283
Hpy8I GTNNAC 3 cut(s) 427, 850, 985
HpyAV CCTTC 2 cut(s) 228, 623
HpyCH4III ACNGT 4 cut(s) 500, 639, 880, 1234
HpyCH4V TGCA 4 cut(s) 110, 698, 1217, 1224
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 794, 842
HpyF3I CTNAG 1 cut(s) 935
Hsp92II CATG 3 cut(s) 127, 874, 1066
KspAI GTTAAC 1 cut(s) 985
Kzo9I GATC 5 cut(s) 148, 857, 961, 998, 1315
LguI GCTCTTC 1 cut(s) 710
Lsp1109I GCAGC 3 cut(s) 584, 848, 1346
LweI GCATC 2 cut(s) 670, 1346
MaeI CTAG 5 cut(s) 176, 929, 1076, 1283, 1328
MaeIII GTNAC 7 cut(s) 305, 401, 633, 754, 974, 1013, 1195
MalI GATC 5 cut(s) 150, 859, 963, 1000, 1317
MboI GATC 5 cut(s) 148, 857, 961, 998, 1315
MboII GAAGA 9 cut(s) 124, 148, 155, 281, 498, 554, 727, 1298, 1301
MflI RGATCY 2 cut(s) 998, 1315
MhlI GDGCHC 3 cut(s) 732, 799, 919
MluCI AATT 7 cut(s) 9, 24, 301, 699, 1206, 1225, 1263
MlyI GAGTC 3 cut(s) 199, 431, 731
MmeI TCCRAC 2 cut(s) 864, 1268
MseI TTAA 3 cut(s) 185, 966, 984
MspCI CTTAAG 2 cut(s) 184, 965
MspI CCGG 3 cut(s) 462, 645, 920
MspR9I CCNGG 3 cut(s) 92, 514, 842
MvaI CCWGG 3 cut(s) 92, 514, 842
MwoI GCNNNNNNNGC 3 cut(s) 79, 794, 842
NcoI CCATGG 1 cut(s) 123
NdeII GATC 5 cut(s) 148, 857, 961, 998, 1315
NlaIII CATG 3 cut(s) 127, 874, 1066
NlaIV GGNNCC 2 cut(s) 918, 1242
NmuCI GTSAC 3 cut(s) 305, 754, 974
NspI RCATGY 1 cut(s) 1066
PaeR7I CTCGAG 2 cut(s) 294, 1132
PciSI GCTCTTC 1 cut(s) 710
PcsI WCGNNNNNNNCGW 1 cut(s) 535
PfeI GAWTC 2 cut(s) 669, 1211
PflMI CCANNNNNTGG 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 90
PkrI GCNGC 4 cut(s) 574, 838, 1333, 1336
PleI GAGTC 3 cut(s) 199, 431, 730
PpsI GAGTC 3 cut(s) 199, 431, 730
Psp6I CCWGG 3 cut(s) 90, 512, 840
PspGI CCWGG 3 cut(s) 90, 512, 840
PspN4I GGNNCC 2 cut(s) 918, 1242
PspPI GGNCC 2 cut(s) 94, 1140
PspXI VCTCGAGB 1 cut(s) 294
PsuI RGATCY 2 cut(s) 998, 1315
RsaI GTAC 2 cut(s) 218, 811
RsaNI GTAC 2 cut(s) 217, 810
SapI GCTCTTC 1 cut(s) 710
SaqAI TTAA 3 cut(s) 185, 966, 984
SatI GCNGC 4 cut(s) 573, 837, 1332, 1335
Sau3AI GATC 5 cut(s) 148, 857, 961, 998, 1315
Sau96I GGNCC 2 cut(s) 94, 1140
SchI GAGTC 3 cut(s) 199, 431, 731
ScrFI CCNGG 3 cut(s) 92, 514, 842
SduI GDGCHC 3 cut(s) 732, 799, 919
SfaNI GCATC 2 cut(s) 670, 1346
SfcI CTRYAG 2 cut(s) 831, 1101
Sfr274I CTCGAG 2 cut(s) 294, 1132
SinI GGWCC 2 cut(s) 94, 1140
SlaI CTCGAG 2 cut(s) 294, 1132
SmlI CTYRAG 6 cut(s) 184, 287, 294, 506, 965, 1132
SmoI CTYRAG 6 cut(s) 184, 287, 294, 506, 965, 1132
Sse9I AATT 7 cut(s) 9, 24, 301, 699, 1206, 1225, 1263
SsiI CCGC 1 cut(s) 1331
SspMI CTAG 5 cut(s) 176, 929, 1076, 1283, 1328
StyD4I CCNGG 3 cut(s) 90, 512, 840
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 4 cut(s) 500, 639, 880, 1234
TaqI TCGA 6 cut(s) 258, 295, 339, 435, 529, 1133
TasI AATT 7 cut(s) 9, 24, 301, 699, 1206, 1225, 1263
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 1334
TfiI GAWTC 2 cut(s) 669, 1211
Tru1I TTAA 3 cut(s) 185, 966, 984
Tru9I TTAA 3 cut(s) 185, 966, 984
TscAI CASTG 4 cut(s) 763, 852, 901, 1239
TseFI GTSAC 3 cut(s) 305, 754, 974
TseI GCWGC 3 cut(s) 572, 836, 1334
Tsp45I GTSAC 3 cut(s) 305, 754, 974
TspDTI ATGAA 3 cut(s) 723, 881, 1071
TspGWI ACGGA 2 cut(s) 715, 877
TspRI CASTG 4 cut(s) 763, 852, 901, 1239
Van91I CCANNNNNTGG 1 cut(s) 230
Vha464I CTTAAG 2 cut(s) 184, 965
VpaK11BI GGWCC 2 cut(s) 94, 1140
XbaI TCTAGA 2 cut(s) 1075, 1282
XceI RCATGY 1 cut(s) 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 1163
XhoI CTCGAG 2 cut(s) 294, 1132
XspI CTAG 5 cut(s) 176, 929, 1076, 1283, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.