FvH4_7g18640

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0006417
Physical Location & Seq
Forward (+)
15502718 .. 15504533
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18640.t1

Sequence Viewer

Length: 1368 bp
ATGGAGAAAATTGAGGTCAGTATAATTTCCAAACACACCATCAAACCATCAACTTCCTCTTCACCACTTCAGCCTTACAAGCTTACCCTGCTCGACCAGCTCACTCCTCCATCGTATGTCCCCATGGTATTCTTCTACCCCATTACTGGCCCTGCAGTCTTCAATCTTCAAACCCTAGCTGACTTAAGACATGCCCTTTCCGAGACTCTCACTTTGTACTATCCACTCTCTGGAAGGGTCAAAAACAACCTATACATCGATGATTTTGAAGAGGGTGTCCCATACCTTGAGGCTCGAGTGAACTGTGACATGAATGATTTTCTAAGGCTTCCGAAAATCGAGTGCCTAAATGAGTTTGTTCCAATAAAACCATTTAGTATGGAAGCAATATCTGATGAGCGTTACCCTTTGCTCGGAGTTCAAGTTAACATTTTCAACTCCGGAATAGCAATCGGGGTCTCCGTCTCTCACAAGCTCATCGATGGAAGAACTTCAGACTGTTTTCTCAAGTCGTGGTGTGCTGTTTTTCGTGGTTCTCGTGACAAAATCATACATCCTAATCTCTCTCAAGCAGCATTGCTTTTCCCACCAAGAGATGACTTGCCTGAAAAGTATGCCCGTCAGATGGAAGGGTTATGGTTTGTCGGAAAAAAAGTTGCTACAAGGAGATTTGTATTTGGTGCGAAAGCCATATCTGTAATTCAAGATGAAGCAAAGAGCGAGTCCGTGCCCAAGCCATCACGAGTTCAGGCTGTCACTAGTTTTCTCTGGAAACATCTAATCGCTACTTCTCGAGCACTAACATCAGGTACTACTTCAACAAGACTTTCTATAGCAACCCAGGTAGTGAACATAAGATCACGGAGGAACATGGAGACAGTGTGGGATAATGCCATTGGAAACTTGATATGGTTCGCTCCGGCCATACTAGAGCTAAGTCATACAACACTAGAGATCAGTGATCTTAAGCTGTGTGACTTGGTTAACTTGCTCAATGGATCTGTCAAACAATGTAACGGTGATTACTTTGAGACTTTCATGGGTAAAGAGGGATATGGAAGCATGTGCGAGTATCTAGATTTTCAGAGGACTATGAGTTCTATGGAACCAGCACCAGAGATTTATTTATTCACGAGCTGGACTAATTTTTTCAACCAACTTGATTTTGGATGGGGGAGGACATCATGGATTGGAGTTGCAGGAAAAATTGAATCTGCATTTTGCAATCTCATAACATTAGTTCCAACACCATGCGATACTGGAATTGAAGCGTGGGTGAATCTAGAAGAAGAAAAAATGGCTATGCTAGAACAAGATCCCCAGTTTCTAGCACTAGCATCTCCAAAGACGCTAATTTCAAGATATTGA

Protein Analysis

456

Amino Acids

51.29

Weight (kDa)

5.82

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 442 2.4e-62 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 230
AccIII TCCGGA 1 cut(s) 440
AclWI GGATC 2 cut(s) 1006, 1310
AcoI YGGCCR 1 cut(s) 921
AcuI CTGAAG 2 cut(s) 53, 477
AfaI GTAC 2 cut(s) 218, 811
AfiI CCNNNNNNNGG 4 cut(s) 146, 230, 413, 625
AflII CTTAAG 2 cut(s) 184, 965
AhlI ACTAGT 1 cut(s) 758
AjnI CCWGG 1 cut(s) 840
AloI GAACNNNNNNTCC 2 cut(s) 1081, 1113
AluBI AGCT 7 cut(s) 82, 100, 179, 475, 934, 970, 1137
AluI AGCT 7 cut(s) 82, 100, 179, 475, 934, 970, 1137
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 5 cut(s) 197, 463, 469, 869, 1025
AlwI GGATC 2 cut(s) 1006, 1310
Ama87I CYCGRG 2 cut(s) 294, 792
Aor13HI TCCGGA 1 cut(s) 440
AoxI GGCC 2 cut(s) 148, 921
ApeKI GCWGC 1 cut(s) 572
ArsI GACNNNNNNTTYG 4 cut(s) 196, 228, 707, 739
Asp700I GAANNNNTTC 1 cut(s) 490
AspS9I GGNCC 1 cut(s) 149
AsuHPI GGTGA 3 cut(s) 54, 1031, 1288
AvaI CYCGRG 2 cut(s) 294, 792
BaeGI GKGCMC 1 cut(s) 732
BauI CACGAG 3 cut(s) 537, 741, 1132
BbsI GAAGAC 1 cut(s) 151
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 1 cut(s) 584
BccI CCATC 7 cut(s) 47, 55, 118, 476, 619, 745, 1164
BciT130I CCWGG 1 cut(s) 842
BcoDI GTCTC 5 cut(s) 197, 463, 469, 869, 1025
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 9 cut(s) 176, 759, 929, 950, 1076, 1283, 1307, 1328, 1334
BfmI CTRYAG 2 cut(s) 153, 831
BfrI CTTAAG 2 cut(s) 184, 965
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme1390I CCNGG 1 cut(s) 842
BmeT110I CYCGRG 2 cut(s) 294, 792
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 1107
BmrFI CCNGG 1 cut(s) 842
BmrI ACTGGG 1 cut(s) 1315
BmsI GCATC 1 cut(s) 1346
BmuI ACTGGG 1 cut(s) 1315
BpiI GAAGAC 1 cut(s) 151
BpuEI CTTGAG 3 cut(s) 308, 491, 552
Bsa29I ATCGAT 2 cut(s) 258, 480
BsaI GGTCTC 1 cut(s) 463
BsaJI CCNNGG 2 cut(s) 123, 840
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 4 cut(s) 408, 438, 866, 896
Bsc4I CCNNNNNNNGG 4 cut(s) 146, 230, 413, 625
Bse1I ACTGG 3 cut(s) 151, 1264, 1321
Bse3DI GCAATG 1 cut(s) 575
BseAI TCCGGA 1 cut(s) 440
BseBI CCWGG 1 cut(s) 842
BseCI ATCGAT 2 cut(s) 258, 480
BseDI CCNNGG 2 cut(s) 123, 840
BseGI GGATG 2 cut(s) 553, 1175
BseLI CCNNNNNNNGG 4 cut(s) 146, 230, 413, 625
BseMI GCAATG 1 cut(s) 575
BseNI ACTGG 3 cut(s) 151, 1264, 1321
BseRI GAGGAG 1 cut(s) 96
BseSI GKGCMC 1 cut(s) 732
BseXI GCAGC 1 cut(s) 584
BshFI GGCC 2 cut(s) 150, 923
BshVI ATCGAT 2 cut(s) 258, 480
BsiHKAI GWGCWC 1 cut(s) 799
BsiHKCI CYCGRG 2 cut(s) 294, 792
BsiSI CCGG 2 cut(s) 441, 920
BslFI GGGAC 2 cut(s) 104, 263
BslI CCNNNNNNNGG 4 cut(s) 146, 230, 413, 625
BsmAI GTCTC 5 cut(s) 197, 463, 469, 869, 1025
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 2 cut(s) 104, 263
BsnI GGCC 2 cut(s) 150, 923
Bso31I GGTCTC 1 cut(s) 463
BsoBI CYCGRG 2 cut(s) 294, 792
Bsp1286I GDGCHC 2 cut(s) 732, 799
Bsp13I TCCGGA 1 cut(s) 440
Bsp143I GATC 5 cut(s) 857, 954, 961, 998, 1315
Bsp19I CCATGG 1 cut(s) 123
BspANI GGCC 2 cut(s) 150, 923
BspDI ATCGAT 2 cut(s) 258, 480
BspEI TCCGGA 1 cut(s) 440
BspLI GGNNCC 1 cut(s) 1107
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 2 cut(s) 1006, 1310
BspTI CTTAAG 2 cut(s) 184, 965
BspTNI GGTCTC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 575
BsrI ACTGG 3 cut(s) 151, 1264, 1321
BssECI CCNNGG 2 cut(s) 123, 840
BssMI GATC 5 cut(s) 857, 954, 961, 998, 1315
BssSI CACGAG 3 cut(s) 537, 741, 1132
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 3 cut(s) 537, 741, 1132
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 4 cut(s) 305, 500, 880, 1019
Bst6I CTCTTC 2 cut(s) 64, 264
BstAFI CTTAAG 2 cut(s) 184, 965
BstDEI CTNAG 2 cut(s) 323, 935
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 2 cut(s) 553, 1175
BstKTI GATC 5 cut(s) 860, 957, 964, 1001, 1318
BstMAI GTCTC 5 cut(s) 197, 463, 469, 869, 1025
BstMBI GATC 5 cut(s) 857, 954, 961, 998, 1315
BstMWI GCNNNNNNNGC 3 cut(s) 79, 88, 97
BstNI CCWGG 1 cut(s) 842
BstNSI RCATGY 2 cut(s) 194, 1066
BstSCI CCNGG 1 cut(s) 840
BstSFI CTRYAG 2 cut(s) 153, 831
BstSLI GKGCMC 1 cut(s) 732
BstV1I GCAGC 1 cut(s) 584
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 2 cut(s) 998, 1315
BstYI RGATCY 2 cut(s) 998, 1315
Bsu15I ATCGAT 2 cut(s) 258, 480
BsuRI GGCC 2 cut(s) 150, 923
BsuTUI ATCGAT 2 cut(s) 258, 480
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 2 cut(s) 553, 1175
BtsIMutI CAGTG 2 cut(s) 885, 964
Cfr13I GGNCC 1 cut(s) 149
ClaI ATCGAT 2 cut(s) 258, 480
CseI GACGC 1 cut(s) 1357
Csp6I GTAC 2 cut(s) 217, 810
CviAII CATG 8 cut(s) 124, 191, 310, 871, 1039, 1063, 1185, 1251
CviQI GTAC 2 cut(s) 217, 810
DdeI CTNAG 2 cut(s) 323, 935
DpnI GATC 5 cut(s) 859, 956, 963, 1000, 1317
DpnII GATC 5 cut(s) 857, 954, 961, 998, 1315
EaeI YGGCCR 1 cut(s) 921
Eam1104I CTCTTC 2 cut(s) 64, 264
EarI CTCTTC 2 cut(s) 64, 264
Eco130I CCWWGG 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 463
Eco57I CTGAAG 2 cut(s) 53, 477
Eco88I CYCGRG 2 cut(s) 294, 792
EcoRII CCWGG 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
Esp3I CGTCTC 1 cut(s) 469
FaeI CATG 8 cut(s) 127, 194, 313, 874, 1042, 1066, 1188, 1254
FaqI GGGAC 2 cut(s) 104, 263
FatI CATG 8 cut(s) 123, 190, 309, 870, 1038, 1062, 1184, 1250
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 2 cut(s) 540, 1182
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 9 cut(s) 176, 759, 929, 950, 1076, 1283, 1307, 1328, 1334
GluI GCNGC 1 cut(s) 573
HaeIII GGCC 2 cut(s) 150, 923
HapII CCGG 2 cut(s) 441, 920
HgaI GACGC 1 cut(s) 1357
Hin1II CATG 8 cut(s) 127, 194, 313, 874, 1042, 1066, 1188, 1254
HincII GTYRAC 2 cut(s) 427, 985
HindII GTYRAC 2 cut(s) 427, 985
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 4 cut(s) 205, 722, 1211, 1279
HpaI GTTAAC 2 cut(s) 427, 985
HpaII CCGG 2 cut(s) 441, 920
HphI GGTGA 3 cut(s) 54, 1031, 1288
Hpy166II GTNNAC 4 cut(s) 301, 427, 850, 985
Hpy188I TCNGA 8 cut(s) 202, 333, 394, 416, 496, 624, 647, 1086
Hpy8I GTNNAC 4 cut(s) 301, 427, 850, 985
HpyAV CCTTC 2 cut(s) 228, 623
HpyCH4III ACNGT 4 cut(s) 305, 500, 880, 1019
HpyCH4V TGCA 4 cut(s) 155, 1199, 1217, 1224
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 88, 97
HpyF3I CTNAG 2 cut(s) 323, 935
Hsp92II CATG 8 cut(s) 127, 194, 313, 874, 1042, 1066, 1188, 1254
Kpn2I TCCGGA 1 cut(s) 440
KspAI GTTAAC 2 cut(s) 427, 985
Kzo9I GATC 5 cut(s) 857, 954, 961, 998, 1315
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 1 cut(s) 584
LweI GCATC 1 cut(s) 1346
MaeI CTAG 9 cut(s) 176, 759, 929, 950, 1076, 1283, 1307, 1328, 1334
MaeIII GTNAC 6 cut(s) 305, 401, 539, 754, 974, 1013
MalI GATC 5 cut(s) 859, 956, 963, 1000, 1317
MboI GATC 5 cut(s) 857, 954, 961, 998, 1315
MboII GAAGA 8 cut(s) 51, 124, 151, 158, 281, 498, 1298, 1301
MflI RGATCY 2 cut(s) 998, 1315
MhlI GDGCHC 2 cut(s) 732, 799
MluCI AATT 7 cut(s) 9, 24, 699, 1144, 1206, 1263, 1353
MlyI GAGTC 2 cut(s) 199, 731
MmeI TCCRAC 2 cut(s) 625, 1268
MnlI CCTC 9 cut(s) 7, 67, 117, 265, 283, 858, 1042, 1080, 1170
MroI TCCGGA 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 490
MseI TTAA 4 cut(s) 185, 426, 966, 984
MspCI CTTAAG 2 cut(s) 184, 965
MspI CCGG 2 cut(s) 441, 920
MspR9I CCNGG 1 cut(s) 842
MvaI CCWGG 1 cut(s) 842
MwoI GCNNNNNNNGC 3 cut(s) 79, 88, 97
NcoI CCATGG 1 cut(s) 123
NdeII GATC 5 cut(s) 857, 954, 961, 998, 1315
NlaIII CATG 8 cut(s) 127, 194, 313, 874, 1042, 1066, 1188, 1254
NlaIV GGNNCC 1 cut(s) 1107
NmuCI GTSAC 4 cut(s) 305, 539, 754, 974
NspI RCATGY 2 cut(s) 194, 1066
PaeR7I CTCGAG 2 cut(s) 294, 792
PcsI WCGNNNNNNNCGW 2 cut(s) 459, 535
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 2 cut(s) 1211, 1279
PflMI CCANNNNNTGG 1 cut(s) 230
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 2 cut(s) 199, 730
PpsI GAGTC 2 cut(s) 199, 730
Psp6I CCWGG 1 cut(s) 840
PspGI CCWGG 1 cut(s) 840
PspN4I GGNNCC 1 cut(s) 1107
PspPI GGNCC 1 cut(s) 149
PspXI VCTCGAGB 1 cut(s) 294
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 2 cut(s) 998, 1315
RsaI GTAC 2 cut(s) 218, 811
RsaNI GTAC 2 cut(s) 217, 810
SaqAI TTAA 4 cut(s) 185, 426, 966, 984
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 5 cut(s) 857, 954, 961, 998, 1315
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 2 cut(s) 199, 731
ScrFI CCNGG 1 cut(s) 842
SduI GDGCHC 2 cut(s) 732, 799
SfaNI GCATC 1 cut(s) 1346
SfcI CTRYAG 2 cut(s) 153, 831
Sfr274I CTCGAG 2 cut(s) 294, 792
SlaI CTCGAG 2 cut(s) 294, 792
SmlI CTYRAG 7 cut(s) 184, 287, 294, 506, 567, 792, 965
SmoI CTYRAG 7 cut(s) 184, 287, 294, 506, 567, 792, 965
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 7 cut(s) 9, 24, 699, 1144, 1206, 1263, 1353
SspMI CTAG 9 cut(s) 176, 759, 929, 950, 1076, 1283, 1307, 1328, 1334
StyD4I CCNGG 1 cut(s) 840
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 4 cut(s) 305, 500, 880, 1019
TaqI TCGA 6 cut(s) 93, 258, 295, 339, 480, 793
TasI AATT 7 cut(s) 9, 24, 699, 1144, 1206, 1263, 1353
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 2 cut(s) 1211, 1279
Tru1I TTAA 4 cut(s) 185, 426, 966, 984
Tru9I TTAA 4 cut(s) 185, 426, 966, 984
TscAI CASTG 2 cut(s) 885, 964
TseFI GTSAC 4 cut(s) 305, 539, 754, 974
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 4 cut(s) 305, 539, 754, 974
TspDTI ATGAA 3 cut(s) 326, 723, 1027
TspGWI ACGGA 3 cut(s) 451, 715, 877
TspRI CASTG 2 cut(s) 885, 964
Van91I CCANNNNNTGG 1 cut(s) 230
Vha464I CTTAAG 2 cut(s) 184, 965
XbaI TCTAGA 2 cut(s) 1075, 1282
XceI RCATGY 2 cut(s) 194, 1066
XcmI CCANNNNNNNNNTGG 1 cut(s) 1163
XhoI CTCGAG 2 cut(s) 294, 792
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 9 cut(s) 176, 759, 929, 950, 1076, 1283, 1307, 1328, 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.