RchiOBHm_Chr2g0101451

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006417
Physical Location & Seq
Forward (+)
13087112 .. 13089344
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47605

Sequence Viewer

Length: 1374 bp
ATGGAGAAAATTGAGGTCAGTATTATTTCCAGAGACGCCATTAAACCATCAGCTGCTTCCTCTTCACTACACCCTTACAAGCTTTCCATCACCGATCAGTTCACTCCCACAATGTATTTCCCAGTTATATTCTTCTACCCCATTACTGACCCTGTCTTCAATCTTCCTCAAACCTTAACCGACTTGAAAAACACTGTTTCCCAGGCTCTCACTTTGTACTATCCACTCTCCGGAAGGATAAAAAACAACCTATACATTGATGATTTCGAAGCAGGCATCCCCTACGTTGAGGCCCGAGTGAATTGTCACATGATTGATTTTCTAAGGCTTCCGAAAATCGAGTGGCTAAATGAGTTTGTTCCAATAGCTCCATATCGCAAGGAAACAATATCTGAGCTTCTTCCCTTGCTTGGAATTCAAGTCAACATTTTCGACTCTGGAATAGCAATCGGCGTCTCTTTCTCTCACAAGATCAACGATGGCGAAACGGCAAACTGTTTTCTCAAGTCCTGGGTTGCTATTTTTCGTGGGTATCGTAACAAAATCATACATCCTAATCTCTCTCAAGCTGCATTACTTTTGCCATCGAGGGATGACTTGCCTGAAAAGTATGTAGCTATGATGGAAAGGTTGTGGTTTGGCGAGAAAAAAGTTGTTACAAGGAGATTTGTATTTGATGCGAAAGCCATATCCGCACTTCAAGATGAAGGGAAGAGCGAGTACGTGCCCAAACCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCTCGGGCATTATCATCAGGTACTTCAACAAGATTTTCCTTCGCATCCCAGGCAGTGAACTTAAGGTCAAAAATGAACATGAAAACGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTCCGGCACTGCTAGATCTAAATGATACAGCACCAGAGAGCAGTGATCTTAAGTTGTGTGACTTGGTTAACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTTTGATGGGTAAAGAGGGATATGGAGGCATGTGTGATTTGCTAGATTTCATGGAAGAGGGGAGTTCTTTTGAACCAGCACCAGAGTTTTATTCATTCTCAAGCTGGACTAGATTTTTTGACCAAGTTGATTTTGGATGGGGGAGGCCATTTTGGGTTGGAGTCTCGGGGAGAGTTGAGACTAATAATTTCACAACATTCGTTGAAACACAATGCGATGATGGAATTGAAGCGTGGGTGACTCTAGATGGAAAACAAATGGCTATGCTAGAACAAGATCCACAGTTTTTAGCATTTGCATCTCCAAACCCGCGAATTTCAATAGCCTCTTCAGTTGGTATTGATTAA

Protein Analysis

457

Amino Acids

51.55

Weight (kDa)

5.03

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 438 6.1e-62 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1339
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 2 cut(s) 693, 1337
AclWI GGATC 1 cut(s) 1298
AcsI RAATTY 3 cut(s) 414, 1004, 1341
AcuI CTGAAG 1 cut(s) 1341
AcvI CACGTG 1 cut(s) 740
AcyI GRCGYC 2 cut(s) 36, 453
AfaI GTAC 3 cut(s) 218, 722, 808
AfiI CCNNNNNNNGG 2 cut(s) 230, 410
AflII CTTAAG 2 cut(s) 847, 959
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 9 cut(s) 160, 187, 419, 701, 813, 1100, 1232, 1256, 1347
AjnI CCWGG 3 cut(s) 201, 509, 834
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 7 cut(s) 53, 82, 368, 397, 569, 617, 1131
AluI AGCT 7 cut(s) 53, 82, 368, 397, 569, 617, 1131
Alw26I GTCTC 5 cut(s) 27, 460, 1019, 1195, 1199
AlwI GGATC 1 cut(s) 1298
Ama87I CYCGRG 3 cut(s) 294, 789, 1192
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 3 cut(s) 291, 747, 1172
ApeKI GCWGC 3 cut(s) 53, 569, 782
ApoI RAATTY 3 cut(s) 414, 1004, 1341
AspS9I GGNCC 2 cut(s) 292, 748
AsuHPI GGTGA 2 cut(s) 82, 1276
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 3 cut(s) 294, 789, 1192
BaeGI GKGCMC 1 cut(s) 729
BanII GRGCYC 1 cut(s) 913
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 40, 556, 769
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 3 cut(s) 203, 511, 836
BcoDI GTCTC 5 cut(s) 27, 460, 1019, 1195, 1199
BfaI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
BfrI CTTAAG 2 cut(s) 847, 959
BglII AGATCT 1 cut(s) 925
BisI GCNGC 3 cut(s) 54, 570, 783
BlsI GCNGC 3 cut(s) 55, 571, 784
Bme1390I CCNGG 3 cut(s) 203, 511, 836
BmeT110I CYCGRG 3 cut(s) 294, 789, 1192
BmgT120I GGNCC 2 cut(s) 292, 748
BmiI GGNNCC 1 cut(s) 912
BmrFI CCNGG 3 cut(s) 203, 511, 836
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 4 cut(s) 285, 667, 839, 1334
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 3 cut(s) 488, 549, 1111
BsaAI YACGTR 2 cut(s) 724, 740
BsaHI GRCGYC 2 cut(s) 36, 453
BsaJI CCNNGG 3 cut(s) 201, 510, 834
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 410
Bse1I ACTGG 2 cut(s) 122, 760
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 3 cut(s) 203, 511, 836
BseDI CCNNGG 3 cut(s) 201, 510, 834
BseGI GGATG 5 cut(s) 276, 550, 598, 830, 1169
BseLI CCNNNNNNNGG 2 cut(s) 230, 410
BseMII CTCAG 1 cut(s) 384
BseNI ACTGG 2 cut(s) 122, 760
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 3 cut(s) 40, 556, 769
Bsh1236I CGCG 1 cut(s) 1339
BshFI GGCC 3 cut(s) 293, 749, 1174
BsiHKCI CYCGRG 3 cut(s) 294, 789, 1192
BsiSI CCGG 2 cut(s) 231, 914
BslI CCNNNNNNNGG 2 cut(s) 230, 410
BsmAI GTCTC 5 cut(s) 27, 460, 1019, 1195, 1199
BsmBI CGTCTC 2 cut(s) 27, 460
BsnI GGCC 3 cut(s) 293, 749, 1174
BsoBI CYCGRG 3 cut(s) 294, 789, 1192
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 2 cut(s) 729, 913
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 5 cut(s) 94, 471, 925, 955, 1303
BspACI CCGC 2 cut(s) 693, 1337
BspANI GGCC 3 cut(s) 293, 749, 1174
BspCNI CTCAG 1 cut(s) 385
BspEI TCCGGA 1 cut(s) 230
BspFNI CGCG 1 cut(s) 1339
BspLI GGNNCC 1 cut(s) 912
BspPI GGATC 1 cut(s) 1298
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 267
BspTI CTTAAG 2 cut(s) 847, 959
BsrI ACTGG 2 cut(s) 122, 760
BssECI CCNNGG 3 cut(s) 201, 510, 834
BssMI GATC 5 cut(s) 94, 471, 925, 955, 1303
BssNI GRCGYC 2 cut(s) 36, 453
Bst2UI CCWGG 3 cut(s) 203, 511, 836
Bst4CI ACNGT 4 cut(s) 196, 497, 1013, 1311
Bst6I CTCTTC 4 cut(s) 67, 707, 1077, 1360
BstACI GRCGYC 2 cut(s) 36, 453
BstAFI CTTAAG 2 cut(s) 847, 959
BstBAI YACGTR 2 cut(s) 724, 740
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 2 cut(s) 323, 393
BstF5I GGATG 5 cut(s) 276, 550, 598, 830, 1169
BstFNI CGCG 1 cut(s) 1339
BstKTI GATC 5 cut(s) 97, 474, 928, 958, 1306
BstMAI GTCTC 5 cut(s) 27, 460, 1019, 1195, 1199
BstMBI GATC 5 cut(s) 94, 471, 925, 955, 1303
BstMWI GCNNNNNNNGC 3 cut(s) 692, 791, 836
BstNI CCWGG 3 cut(s) 203, 511, 836
BstNSI RCATGY 1 cut(s) 1060
BstSCI CCNGG 3 cut(s) 201, 509, 834
BstSLI GKGCMC 1 cut(s) 729
BstUI CGCG 1 cut(s) 1339
BstV1I GCAGC 3 cut(s) 40, 556, 769
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 2 cut(s) 925, 1303
BstYI RGATCY 2 cut(s) 925, 1303
BsuRI GGCC 3 cut(s) 293, 749, 1174
BtgZI GCGATG 1 cut(s) 1257
BtsCI GGATG 5 cut(s) 276, 550, 598, 830, 1169
BtsI GCAGTG 3 cut(s) 846, 917, 958
BtsIMutI CAGTG 6 cut(s) 192, 753, 846, 917, 958, 1018
Cac8I GCNNGC 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 292, 748
CseI GACGC 2 cut(s) 44, 442
Csp6I GTAC 3 cut(s) 217, 721, 807
CviAII CATG 4 cut(s) 310, 865, 1057, 1078
CviQI GTAC 3 cut(s) 217, 721, 807
DdeI CTNAG 2 cut(s) 323, 393
DpnI GATC 5 cut(s) 96, 473, 927, 957, 1305
DpnII GATC 5 cut(s) 94, 471, 925, 955, 1303
Eam1104I CTCTTC 4 cut(s) 67, 707, 1077, 1360
EarI CTCTTC 4 cut(s) 67, 707, 1077, 1360
Eco24I GRGCYC 1 cut(s) 913
Eco57I CTGAAG 1 cut(s) 1341
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 3 cut(s) 294, 789, 1192
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 3 cut(s) 201, 509, 834
EcoT38I GRGCYC 1 cut(s) 913
Esp3I CGTCTC 2 cut(s) 27, 460
FaeI CATG 4 cut(s) 313, 868, 1060, 1081
FatI CATG 4 cut(s) 309, 864, 1056, 1077
FauI CCCGC 1 cut(s) 1344
Fnu4HI GCNGC 3 cut(s) 54, 570, 783
FokI GGATG 5 cut(s) 263, 537, 605, 817, 1176
FriOI GRGCYC 1 cut(s) 913
Fsp4HI GCNGC 3 cut(s) 54, 570, 783
FspBI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
GluI GCNGC 3 cut(s) 54, 570, 783
HaeIII GGCC 3 cut(s) 293, 749, 1174
HapII CCGG 2 cut(s) 231, 914
HgaI GACGC 2 cut(s) 44, 442
Hin1I GRCGYC 2 cut(s) 36, 453
Hin1II CATG 4 cut(s) 313, 868, 1060, 1081
HincII GTYRAC 2 cut(s) 424, 979
HindII GTYRAC 2 cut(s) 424, 979
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 4 cut(s) 434, 992, 1188, 1267
HpaI GTTAAC 1 cut(s) 979
HpaII CCGG 2 cut(s) 231, 914
HphI GGTGA 2 cut(s) 82, 1276
Hpy166II GTNNAC 4 cut(s) 102, 424, 844, 979
Hpy188I TCNGA 2 cut(s) 333, 394
Hpy188III TCNNGA 6 cut(s) 30, 231, 438, 701, 766, 1271
Hpy8I GTNNAC 4 cut(s) 102, 424, 844, 979
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 3 cut(s) 228, 701, 835
HpyCH4III ACNGT 4 cut(s) 196, 497, 1013, 1311
HpyCH4IV ACGT 4 cut(s) 285, 723, 739, 875
HpyCH4V TGCA 2 cut(s) 572, 1325
HpyF10VI GCNNNNNNNGC 3 cut(s) 692, 791, 836
HpyF3I CTNAG 2 cut(s) 323, 393
HpySE526I ACGT 4 cut(s) 285, 723, 739, 875
Hsp92I GRCGYC 2 cut(s) 36, 453
Hsp92II CATG 4 cut(s) 313, 868, 1060, 1081
Kpn2I TCCGGA 1 cut(s) 230
KspAI GTTAAC 1 cut(s) 979
Kzo9I GATC 5 cut(s) 94, 471, 925, 955, 1303
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 2 cut(s) 373, 916
Lsp1109I GCAGC 3 cut(s) 40, 556, 769
LweI GCATC 4 cut(s) 285, 667, 839, 1334
MaeI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
MaeII ACGT 4 cut(s) 285, 723, 739, 875
MaeIII GTNAC 5 cut(s) 305, 536, 655, 968, 1264
MalI GATC 5 cut(s) 96, 473, 927, 957, 1305
MboI GATC 5 cut(s) 94, 471, 925, 955, 1303
MboII GAAGA 8 cut(s) 54, 124, 148, 155, 392, 724, 1094, 1347
MflI RGATCY 2 cut(s) 925, 1303
MhlI GDGCHC 2 cut(s) 729, 913
MluCI AATT 7 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341
MlyI GAGTC 3 cut(s) 428, 1197, 1261
MmeI TCCRAC 2 cut(s) 858, 1165
MroI TCCGGA 1 cut(s) 230
MseI TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 2 cut(s) 847, 959
MspI CCGG 2 cut(s) 231, 914
MspR9I CCNGG 3 cut(s) 203, 511, 836
MvaI CCWGG 3 cut(s) 203, 511, 836
MvnI CGCG 1 cut(s) 1339
MwoI GCNNNNNNNGC 3 cut(s) 692, 791, 836
NdeII GATC 5 cut(s) 94, 471, 925, 955, 1303
NlaIII CATG 4 cut(s) 313, 868, 1060, 1081
NlaIV GGNNCC 1 cut(s) 912
NmuCI GTSAC 3 cut(s) 305, 968, 1264
NspI RCATGY 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PfeI GAWTC 1 cut(s) 992
PflFI GACNNNGTC 1 cut(s) 152
PkrI GCNGC 3 cut(s) 55, 571, 784
PleI GAGTC 3 cut(s) 428, 1196, 1261
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 3 cut(s) 428, 1196, 1261
Ppu21I YACGTR 2 cut(s) 724, 740
Psp6I CCWGG 3 cut(s) 201, 509, 834
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 3 cut(s) 201, 509, 834
PspN4I GGNNCC 1 cut(s) 912
PspPI GGNCC 2 cut(s) 292, 748
PsuI RGATCY 2 cut(s) 925, 1303
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 3 cut(s) 218, 722, 808
RsaNI GTAC 3 cut(s) 217, 721, 807
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
SatI GCNGC 3 cut(s) 54, 570, 783
Sau3AI GATC 5 cut(s) 94, 471, 925, 955, 1303
Sau96I GGNCC 2 cut(s) 292, 748
SchI GAGTC 3 cut(s) 428, 1197, 1261
ScrFI CCNGG 3 cut(s) 203, 511, 836
SduI GDGCHC 2 cut(s) 729, 913
SfaNI GCATC 4 cut(s) 285, 667, 839, 1334
SfuI TTCGAA 1 cut(s) 267
SmlI CTYRAG 5 cut(s) 503, 564, 847, 959, 1126
SmoI CTYRAG 5 cut(s) 503, 564, 847, 959, 1126
Sse9I AATT 7 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341
SsiI CCGC 2 cut(s) 693, 1337
SspMI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
StyD4I CCNGG 3 cut(s) 201, 509, 834
TaaI ACNGT 4 cut(s) 196, 497, 1013, 1311
TaiI ACGT 4 cut(s) 288, 726, 742, 878
TaqI TCGA 4 cut(s) 267, 339, 432, 587
TasI AATT 7 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 992
Tru1I TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
Tru9I TTAA 7 cut(s) 42, 176, 848, 960, 978, 1008, 1372
TscAI CASTG 6 cut(s) 199, 760, 846, 924, 958, 1018
TseFI GTSAC 3 cut(s) 305, 968, 1264
TseI GCWGC 3 cut(s) 53, 569, 782
Tsp45I GTSAC 3 cut(s) 305, 968, 1264
TspDTI ATGAA 6 cut(s) 720, 875, 881, 1005, 1066, 1110
TspRI CASTG 6 cut(s) 199, 760, 846, 924, 958, 1018
Tth111I GACNNNGTC 1 cut(s) 152
Vha464I CTTAAG 2 cut(s) 847, 959
XapI RAATTY 3 cut(s) 414, 1004, 1341
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 1 cut(s) 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 1157
XspI CTAG 5 cut(s) 923, 1070, 1137, 1271, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.