RLG00000017082

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006417
Physical Location & Seq
Forward (+)
12684678 .. 12686780
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017082

Sequence Viewer

Length: 1398 bp
ATGGAGAAAATTGAGGTCAGTATTATTTCCAGAGACACCATTAAACCATCAGCTGCTTCCTCTTCACTACACCCTTACAAGCTTTCCATCATCGATCAGTTCACTCCCACAACGTATTTCCCAGTTATATTCTTCTACCCCATTACTGACCCTGTCTTCAATCTCGCTCAAACCTTAACCGACTTGAAAATCACTGTTTCCCAGGCTCTCACTTTGTACTATCCACTCTCCGGAAGGATAAAAAACAACCTATACATTGATGATTTCGAAGCAGGCATCCCCTACCTTGAGGCCCGAGTGAATTGTCACATGATTGATTTTATAAGGCTTCCGAAAATCGAGTGGCTAAATGAGTTTGTTCCAATGGCTCCATATCGCAAGGAAACAATATCTGAGCTTCTTCCCTTGCTTGGAATTCAAGTCAACATTTTCGACTCTGGAATAGCAATCGGTGTCTCGTTTTCTCACAAGATCAACGATGGCGAAACGGCAAACTGCTTTCTCAAGTCCTGGGTTGCTATTTTTCGTGGGTATCGTAACAAAATCATACATCCTAATCTCTCTCAAGCTGCATTACTTTTGCCATCGAGGGATGACTTGCCTGAAAAGTACGTAGCTATGATGGAAAGGTTGTGGTTTGGCGAGAAAAAAGTTGTTACAAAGAGATTTGTATTTGATGCGAAAGCCATATCCGCACTTCAAGATGAAGGGAAGAGCAAGTACGTGCCCAAACCATCACGTGTTCAGGCCCTCACTGGTTTTCTCTGGAAACATCAACTCGCTGCTTCTCGGGCATTATCATCAGGTACTTCAACAAGATTTTCCGTAGCATCTCAGACAGTGAACTTAAGGTCAAAAATGAATATGAAAACGACGTTGGACAATGCCATTGGTAATATCTTTTTGTGGGCTTCGGCACAGCTAGATCTAAATGATACAGCACCAGGGAGCAGTGATCTTAAGTTGTGTGACTTGGTTAACTTACTCAATGAATCTATCAAAGAATTTAACAGTGATTACTTGGAGACTCTGAAGGGTAAAGAGGGATATGGAGGCATGTGTGATTTGCTAGATCTCATGGAAGAGGGGAGTTCTGTAGAACCAGCACCAGAGTTTTATTCATTCTCAAGCTGGACTAGATTTTTTGACCAAGTTGATTTTGGATGGGGGAGGCCATCTTGGGTTGGAGTCTCAGGGAGAGTTGGAACTAGAAATTTCACAACATTCGTTGAAACACAATGCGATGACGGAATTGAAGCGTGGGTGACTCTAGATGAAAAACAAATGGCTATGCTAGAAAAAGATCCACAGTTTTTAGCATATGCATCTCCAAACCCCGGAATTTCAATAGCCTCTTCAATTGGTATTGATCAAGTGTTTCCATTTTGTGGTGAATAA

Protein Analysis

466

Amino Acids

52.19

Weight (kDa)

5.3

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 438 1.1e-61 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 1388
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 1 cut(s) 693
AclWI GGATC 1 cut(s) 1298
AcsI RAATTY 4 cut(s) 414, 1004, 1213, 1341
AcuI CTGAAG 1 cut(s) 1052
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 4 cut(s) 218, 611, 722, 808
AfiI CCNNNNNNNGG 4 cut(s) 230, 410, 1337, 1388
AflII CTTAAG 2 cut(s) 847, 959
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 9 cut(s) 160, 187, 419, 701, 813, 1232, 1256, 1347, 1359
AjnI CCWGG 3 cut(s) 201, 509, 943
AjuI GAANNNNNNNTTGG 2 cut(s) 860, 892
AluBI AGCT 7 cut(s) 53, 82, 397, 569, 617, 922, 1131
AluI AGCT 7 cut(s) 53, 82, 397, 569, 617, 922, 1131
Alw26I GTCTC 4 cut(s) 27, 460, 1019, 1195
AlwI GGATC 1 cut(s) 1298
Ama87I CYCGRG 2 cut(s) 294, 789
Aor13HI TCCGGA 1 cut(s) 230
AoxI GGCC 3 cut(s) 291, 747, 1172
ApeKI GCWGC 3 cut(s) 53, 569, 782
ApoI RAATTY 4 cut(s) 414, 1004, 1213, 1341
AspS9I GGNCC 2 cut(s) 292, 748
AsuC2I CCSGG 1 cut(s) 1338
AsuHPI GGTGA 1 cut(s) 1276
AsuII TTCGAA 1 cut(s) 267
AvaI CYCGRG 2 cut(s) 294, 789
BaeGI GKGCMC 1 cut(s) 729
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 40, 556, 769
BccI CCATC 8 cut(s) 55, 95, 473, 592, 616, 742, 1158, 1183
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 3 cut(s) 203, 511, 945
BclI TGATCA 1 cut(s) 1369
BcnI CCSGG 1 cut(s) 1338
BcoDI GTCTC 4 cut(s) 27, 460, 1019, 1195
BfaI CTAG 6 cut(s) 923, 1070, 1137, 1209, 1271, 1295
BfmI CTRYAG 1 cut(s) 1095
BfrI CTTAAG 2 cut(s) 847, 959
BglII AGATCT 2 cut(s) 925, 1072
BisI GCNGC 3 cut(s) 54, 570, 783
BlsI GCNGC 3 cut(s) 55, 571, 784
Bme1390I CCNGG 4 cut(s) 203, 511, 945, 1338
BmeT110I CYCGRG 2 cut(s) 294, 789
BmgT120I GGNCC 2 cut(s) 292, 748
BmiI GGNNCC 1 cut(s) 369
BmrFI CCNGG 4 cut(s) 203, 511, 945, 1338
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 4 cut(s) 285, 667, 839, 1334
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 148
Bpu14I TTCGAA 1 cut(s) 267
BpuEI CTTGAG 4 cut(s) 308, 488, 549, 1111
BpuMI CCSGG 1 cut(s) 1338
Bsa29I ATCGAT 1 cut(s) 93
BsaAI YACGTR 3 cut(s) 613, 724, 740
BsaJI CCNNGG 4 cut(s) 201, 510, 944, 1336
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 410, 1337, 1388
Bse1I ACTGG 2 cut(s) 122, 760
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 3 cut(s) 203, 511, 945
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 4 cut(s) 201, 510, 944, 1336
BseGI GGATG 4 cut(s) 276, 550, 598, 1169
BseLI CCNNNNNNNGG 4 cut(s) 230, 410, 1337, 1388
BseMII CTCAG 3 cut(s) 384, 848, 1206
BseNI ACTGG 2 cut(s) 122, 760
BseSI GKGCMC 1 cut(s) 729
BseXI GCAGC 3 cut(s) 40, 556, 769
BshFI GGCC 3 cut(s) 293, 749, 1174
BshVI ATCGAT 1 cut(s) 93
BsiHKCI CYCGRG 2 cut(s) 294, 789
BsiSI CCGG 2 cut(s) 231, 1338
BslI CCNNNNNNNGG 4 cut(s) 230, 410, 1337, 1388
BsmAI GTCTC 4 cut(s) 27, 460, 1019, 1195
BsnI GGCC 3 cut(s) 293, 749, 1174
BsoBI CYCGRG 2 cut(s) 294, 789
Bsp119I TTCGAA 1 cut(s) 267
Bsp1286I GDGCHC 1 cut(s) 729
Bsp13I TCCGGA 1 cut(s) 230
Bsp143I GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
BspACI CCGC 1 cut(s) 693
BspANI GGCC 3 cut(s) 293, 749, 1174
BspCNI CTCAG 3 cut(s) 385, 847, 1205
BspDI ATCGAT 1 cut(s) 93
BspEI TCCGGA 1 cut(s) 230
BspLI GGNNCC 1 cut(s) 369
BspPI GGATC 1 cut(s) 1298
BspQI GCTCTTC 1 cut(s) 707
BspT104I TTCGAA 1 cut(s) 267
BspTI CTTAAG 2 cut(s) 847, 959
BsrI ACTGG 2 cut(s) 122, 760
BssECI CCNNGG 4 cut(s) 201, 510, 944, 1336
BssMI GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
Bst2UI CCWGG 3 cut(s) 203, 511, 945
Bst4CI ACNGT 4 cut(s) 196, 841, 1013, 1311
Bst6I CTCTTC 4 cut(s) 67, 707, 1077, 1360
BstAFI CTTAAG 2 cut(s) 847, 959
BstBAI YACGTR 3 cut(s) 613, 724, 740
BstBI TTCGAA 1 cut(s) 267
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 3 cut(s) 393, 834, 1192
BstF5I GGATG 4 cut(s) 276, 550, 598, 1169
BstKTI GATC 7 cut(s) 97, 474, 928, 958, 1075, 1306, 1372
BstMAI GTCTC 4 cut(s) 27, 460, 1019, 1195
BstMBI GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
BstMWI GCNNNNNNNGC 2 cut(s) 692, 791
BstNI CCWGG 3 cut(s) 203, 511, 945
BstNSI RCATGY 1 cut(s) 1060
BstSCI CCNGG 4 cut(s) 201, 509, 943, 1336
BstSFI CTRYAG 1 cut(s) 1095
BstSLI GKGCMC 1 cut(s) 729
BstSNI TACGTA 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 40, 556, 769
BstV2I GAAGAC 1 cut(s) 148
BstX2I RGATCY 3 cut(s) 925, 1072, 1303
BstYI RGATCY 3 cut(s) 925, 1072, 1303
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 3 cut(s) 293, 749, 1174
BsuTUI ATCGAT 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 1257
BtsCI GGATG 4 cut(s) 276, 550, 598, 1169
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 5 cut(s) 192, 753, 846, 958, 1018
Cac8I GCNNGC 1 cut(s) 274
Cfr13I GGNCC 2 cut(s) 292, 748
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 4 cut(s) 217, 610, 721, 807
CviAII CATG 3 cut(s) 310, 1057, 1078
CviQI GTAC 4 cut(s) 217, 610, 721, 807
DdeI CTNAG 3 cut(s) 393, 834, 1192
DpnI GATC 7 cut(s) 96, 473, 927, 957, 1074, 1305, 1371
DpnII GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
Eam1104I CTCTTC 4 cut(s) 67, 707, 1077, 1360
EarI CTCTTC 4 cut(s) 67, 707, 1077, 1360
Eco105I TACGTA 1 cut(s) 613
Eco57I CTGAAG 1 cut(s) 1052
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 2 cut(s) 294, 789
EcoO109I RGGNCCY 1 cut(s) 748
EcoRI GAATTC 1 cut(s) 414
EcoRII CCWGG 3 cut(s) 201, 509, 943
EcoT22I ATGCAT 1 cut(s) 1327
FaeI CATG 3 cut(s) 313, 1060, 1081
FatI CATG 3 cut(s) 309, 1056, 1077
FauNDI CATATG 1 cut(s) 1321
FbaI TGATCA 1 cut(s) 1369
Fnu4HI GCNGC 3 cut(s) 54, 570, 783
FokI GGATG 4 cut(s) 263, 537, 605, 1176
Fsp4HI GCNGC 3 cut(s) 54, 570, 783
FspBI CTAG 6 cut(s) 923, 1070, 1137, 1209, 1271, 1295
GluI GCNGC 3 cut(s) 54, 570, 783
HaeIII GGCC 3 cut(s) 293, 749, 1174
HapII CCGG 2 cut(s) 231, 1338
Hin1II CATG 3 cut(s) 313, 1060, 1081
HincII GTYRAC 2 cut(s) 424, 979
HindII GTYRAC 2 cut(s) 424, 979
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 5 cut(s) 434, 992, 1027, 1188, 1267
HpaI GTTAAC 1 cut(s) 979
HpaII CCGG 2 cut(s) 231, 1338
HphI GGTGA 1 cut(s) 1276
Hpy166II GTNNAC 4 cut(s) 102, 424, 844, 979
Hpy188I TCNGA 4 cut(s) 333, 394, 837, 1032
Hpy188III TCNNGA 6 cut(s) 30, 231, 438, 701, 766, 1271
Hpy8I GTNNAC 4 cut(s) 102, 424, 844, 979
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 3 cut(s) 228, 701, 1027
HpyCH4III ACNGT 4 cut(s) 196, 841, 1013, 1311
HpyCH4IV ACGT 5 cut(s) 113, 612, 723, 739, 875
HpyCH4V TGCA 2 cut(s) 572, 1325
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 791
HpyF3I CTNAG 3 cut(s) 393, 834, 1192
HpySE526I ACGT 5 cut(s) 113, 612, 723, 739, 875
Hsp92II CATG 3 cut(s) 313, 1060, 1081
Kpn2I TCCGGA 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 1369
KspAI GTTAAC 1 cut(s) 979
Kzo9I GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
LguI GCTCTTC 1 cut(s) 707
LmnI GCTCC 2 cut(s) 373, 948
Lsp1109I GCAGC 3 cut(s) 40, 556, 769
LweI GCATC 4 cut(s) 285, 667, 839, 1334
MaeI CTAG 6 cut(s) 923, 1070, 1137, 1209, 1271, 1295
MaeII ACGT 5 cut(s) 113, 612, 723, 739, 875
MaeIII GTNAC 5 cut(s) 305, 536, 655, 968, 1264
MalI GATC 7 cut(s) 96, 473, 927, 957, 1074, 1305, 1371
MboI GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
MboII GAAGA 7 cut(s) 54, 124, 148, 392, 724, 1094, 1347
MfeI CAATTG 1 cut(s) 1359
MflI RGATCY 3 cut(s) 925, 1072, 1303
MhlI GDGCHC 1 cut(s) 729
MluCI AATT 8 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341, 1359
MlyI GAGTC 4 cut(s) 428, 1021, 1197, 1261
MmeI TCCRAC 3 cut(s) 858, 1165, 1183
Mph1103I ATGCAT 1 cut(s) 1327
MroI TCCGGA 1 cut(s) 230
MseI TTAA 6 cut(s) 42, 176, 848, 960, 978, 1008
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 2 cut(s) 847, 959
MspI CCGG 2 cut(s) 231, 1338
MspR9I CCNGG 4 cut(s) 203, 511, 945, 1338
MunI CAATTG 1 cut(s) 1359
MvaI CCWGG 3 cut(s) 203, 511, 945
MwoI GCNNNNNNNGC 2 cut(s) 692, 791
NciI CCSGG 1 cut(s) 1338
NdeI CATATG 1 cut(s) 1321
NdeII GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
NlaIII CATG 3 cut(s) 313, 1060, 1081
NlaIV GGNNCC 1 cut(s) 369
NmuCI GTSAC 3 cut(s) 305, 968, 1264
NsiI ATGCAT 1 cut(s) 1327
NspI RCATGY 1 cut(s) 1060
NspV TTCGAA 1 cut(s) 267
PciSI GCTCTTC 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PfeI GAWTC 1 cut(s) 992
PflFI GACNNNGTC 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 1388
PkrI GCNGC 3 cut(s) 55, 571, 784
PleI GAGTC 4 cut(s) 428, 1021, 1196, 1261
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 4 cut(s) 428, 1021, 1196, 1261
Ppu21I YACGTR 3 cut(s) 613, 724, 740
PsiI TTATAA 1 cut(s) 323
Psp6I CCWGG 3 cut(s) 201, 509, 943
PspCI CACGTG 1 cut(s) 740
PspGI CCWGG 3 cut(s) 201, 509, 943
PspN4I GGNNCC 1 cut(s) 369
PspPI GGNCC 2 cut(s) 292, 748
PsuI RGATCY 3 cut(s) 925, 1072, 1303
PsyI GACNNNGTC 1 cut(s) 152
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 4 cut(s) 218, 611, 722, 808
RsaNI GTAC 4 cut(s) 217, 610, 721, 807
SapI GCTCTTC 1 cut(s) 707
SaqAI TTAA 6 cut(s) 42, 176, 848, 960, 978, 1008
SatI GCNGC 3 cut(s) 54, 570, 783
Sau3AI GATC 7 cut(s) 94, 471, 925, 955, 1072, 1303, 1369
Sau96I GGNCC 2 cut(s) 292, 748
SchI GAGTC 4 cut(s) 428, 1021, 1197, 1261
ScrFI CCNGG 4 cut(s) 203, 511, 945, 1338
SduI GDGCHC 1 cut(s) 729
SfaNI GCATC 4 cut(s) 285, 667, 839, 1334
SfcI CTRYAG 1 cut(s) 1095
SfuI TTCGAA 1 cut(s) 267
SmlI CTYRAG 6 cut(s) 287, 503, 564, 847, 959, 1126
SmoI CTYRAG 6 cut(s) 287, 503, 564, 847, 959, 1126
SnaBI TACGTA 1 cut(s) 613
Sse9I AATT 8 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341, 1359
SsiI CCGC 1 cut(s) 693
SspMI CTAG 6 cut(s) 923, 1070, 1137, 1209, 1271, 1295
StyD4I CCNGG 4 cut(s) 201, 509, 943, 1336
TaaI ACNGT 4 cut(s) 196, 841, 1013, 1311
TaiI ACGT 5 cut(s) 116, 615, 726, 742, 878
TaqI TCGA 5 cut(s) 93, 267, 339, 432, 587
TasI AATT 8 cut(s) 9, 301, 414, 1004, 1213, 1251, 1341, 1359
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 992
Tru1I TTAA 6 cut(s) 42, 176, 848, 960, 978, 1008
Tru9I TTAA 6 cut(s) 42, 176, 848, 960, 978, 1008
TscAI CASTG 5 cut(s) 199, 760, 846, 958, 1018
TseFI GTSAC 3 cut(s) 305, 968, 1264
TseI GCWGC 3 cut(s) 53, 569, 782
Tsp45I GTSAC 3 cut(s) 305, 968, 1264
TspDTI ATGAA 6 cut(s) 720, 875, 881, 1005, 1110, 1290
TspGWI ACGGA 2 cut(s) 814, 1263
TspRI CASTG 5 cut(s) 199, 760, 846, 958, 1018
Tth111I GACNNNGTC 1 cut(s) 152
Van91I CCANNNNNTGG 1 cut(s) 1388
Vha464I CTTAAG 2 cut(s) 847, 959
XapI RAATTY 4 cut(s) 414, 1004, 1213, 1341
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 1 cut(s) 1060
XcmI CCANNNNNNNNNTGG 1 cut(s) 1157
XspI CTAG 6 cut(s) 923, 1070, 1137, 1209, 1271, 1295
Zsp2I ATGCAT 1 cut(s) 1327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.