FvH4_6g41871

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004903
Physical Location & Seq
Forward (+)
32793094 .. 32795503
2410 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41871.t2

Sequence Viewer

Length: 1053 bp
ATGGCATGCATGTACGTTCCATGGCTCTTGTTGACTCAAATCCTACTACAAGTAGCAAAACACGATGCCAAGGTTCCAGCCATTATTGTGTTTGGAGACTCAACTGTGGATTCAGGAAACAACAACCGGATTTCGACACTCTTGAAGAGCAACTTCAAGCCATATGGGCGTGATTTTGCTGGGGGTCAACCCACAGGAAGGTTTTCCAATGGTCGTGTTCCTCCGGATTTCATTTCTGAGGCTTTTGGACTCCAACCATTCGTTCCGGCTTACTTGGATCCCAGTTTTGGGATTTCGGATTTTGTCACTGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGACAATGCTACATCTGACGTACTGAATGTGATACCTCTGTGGAAAGAAGTGGAGTACTACAAGGAGTACCAAAGCAAATTGGGAGACTATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAGGCTCTGTATTTGATAAGCTTGGGAACCAATGATTTCCTTGAGAACTACTACACGATTCCTCGCCGGCGAGTCCAATTCACAGTCCAGCAATACCAGGATTTCCTGGTGGGACTTGCTGAGACTTTCATGAGGGAGATTTACAGTCTTGGAGTCAGAAAAGTTTCCCTAACTGGGCTCCCTCCCATGGGGTGCTTGCCCTTAGAGAGGGCCACAAATTTCATGAACCATCATGATTGTGTGGAGGAATATAACAAGGTGGCTGTGGAATTCAATGGGAAGTTGAACAACATGGTAGCACAACTGAATCAAGAGCTTCCAGGCTATACAATTATATTTACTGCAAAAGTATATCAGTTGCTCTATCAAATCATTAGCAGCCCTTCTTCATATGGATTTGATGTTGCTGAAGTGGCATGTTGCTCTACTGGGCTATTTGAGATGAGTTACCTCTGCAATGAGCATAATCCATTTACATGCACAGATGCAAATAAGTATATATTTTGGGATGCCTTTCATCCTACTGACAAAACAAATCAGATAATAGCTAGCTATTTGATTCCAGATCTTCTAAAATTGTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

39.45

Weight (kDa)

5.28

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 268 6.3e-27 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 223
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 3 cut(s) 456, 682, 734
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 4 cut(s) 14, 363, 398, 410
AfiI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 672
AgsI TTSAA 5 cut(s) 145, 157, 739, 751, 1048
AjnI CCWGG 3 cut(s) 561, 570, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 246, 278
AluBI AGCT 4 cut(s) 486, 781, 1013, 1017
AluI AGCT 4 cut(s) 486, 781, 1013, 1017
Alw26I GTCTC 3 cut(s) 90, 420, 581
AlwI GGATC 2 cut(s) 272, 285
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 2 cut(s) 436, 675
ApeKI GCWGC 1 cut(s) 843
ApoI RAATTY 3 cut(s) 456, 682, 734
Asp700I GAANNNNTTC 2 cut(s) 202, 628
AspS9I GGNCC 1 cut(s) 675
AsuNHI GCTAGC 1 cut(s) 1013
BaeI ACNNNNGTAYC 2 cut(s) 365, 398
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 645
BbvI GCAGC 1 cut(s) 855
BccI CCATC 2 cut(s) 447, 702
BciT130I CCWGG 3 cut(s) 563, 572, 786
BcoDI GTCTC 3 cut(s) 90, 420, 581
BfaI CTAG 1 cut(s) 1014
BglI GCCNNNNNGGC 1 cut(s) 166
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 1 cut(s) 844
BlsI GCNGC 1 cut(s) 845
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 563, 572, 786
BmgT120I GGNCC 1 cut(s) 675
BmiI GGNNCC 4 cut(s) 75, 279, 493, 644
BmrFI CCNGG 3 cut(s) 563, 572, 786
BmrI ACTGGG 3 cut(s) 276, 648, 903
BmsI GCATC 3 cut(s) 55, 940, 964
BmtI GCTAGC 1 cut(s) 1017
BmuI ACTGGG 3 cut(s) 276, 648, 903
BpuEI CTTGAG 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 20, 69, 651
BsaWI WCCGGW 2 cut(s) 126, 223
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 672
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 5 cut(s) 282, 313, 340, 643, 898
Bse3DI GCAATG 1 cut(s) 928
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 3 cut(s) 563, 572, 786
BseDI CCNNGG 3 cut(s) 20, 69, 651
BseGI GGATG 2 cut(s) 979, 982
BseLI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 672
BseMI GCAATG 1 cut(s) 928
BseMII CTCAG 2 cut(s) 228, 576
BseNI ACTGG 5 cut(s) 282, 313, 340, 643, 898
BseXI GCAGC 1 cut(s) 855
BseYI CCCAGC 1 cut(s) 179
BshFI GGCC 2 cut(s) 438, 677
BsiSI CCGG 4 cut(s) 127, 224, 266, 532
BslFI GGGAC 1 cut(s) 591
BslI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 672
BsmAI GTCTC 3 cut(s) 90, 420, 581
BsmFI GGGAC 1 cut(s) 591
BsnI GGCC 2 cut(s) 438, 677
Bsp1286I GDGCHC 1 cut(s) 645
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 2 cut(s) 277, 1030
Bsp19I CCATGG 2 cut(s) 20, 651
BspANI GGCC 2 cut(s) 438, 677
BspCNI CTCAG 2 cut(s) 229, 577
BspEI TCCGGA 1 cut(s) 223
BspHI TCATGA 3 cut(s) 594, 687, 697
BspLI GGNNCC 4 cut(s) 75, 279, 493, 644
BspOI GCTAGC 1 cut(s) 1017
BspPI GGATC 2 cut(s) 272, 285
BspQI GCTCTTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 928
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 5 cut(s) 282, 313, 340, 643, 898
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 20, 69, 651
BssMI GATC 2 cut(s) 277, 1030
BssT1I CCWWGG 3 cut(s) 20, 69, 651
Bst2UI CCWGG 3 cut(s) 563, 572, 786
Bst4CI ACNGT 3 cut(s) 106, 550, 611
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 4 cut(s) 7, 533, 662, 1015
BstDEI CTNAG 3 cut(s) 237, 585, 667
BstDSI CCRYGG 2 cut(s) 20, 651
BstENI CCTNNNNNAGG 1 cut(s) 670
BstF5I GGATG 2 cut(s) 979, 982
BstKTI GATC 2 cut(s) 280, 1033
BstMAI GTCTC 3 cut(s) 90, 420, 581
BstMBI GATC 2 cut(s) 277, 1030
BstMWI GCNNNNNNNGC 2 cut(s) 166, 878
BstNI CCWGG 3 cut(s) 563, 572, 786
BstNSI RCATGY 4 cut(s) 9, 13, 885, 945
BstSCI CCNGG 3 cut(s) 561, 570, 784
BstV1I GCAGC 1 cut(s) 855
BstX2I RGATCY 2 cut(s) 277, 1030
BstYI RGATCY 2 cut(s) 277, 1030
BsuRI GGCC 2 cut(s) 438, 677
BtgI CCRYGG 2 cut(s) 20, 651
BtsCI GGATG 2 cut(s) 979, 982
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 4 cut(s) 7, 533, 662, 1015
CciI TCATGA 3 cut(s) 594, 687, 697
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 675
Csp6I GTAC 4 cut(s) 13, 362, 397, 409
CviQI GTAC 4 cut(s) 13, 362, 397, 409
DdeI CTNAG 3 cut(s) 237, 585, 667
DpnI GATC 2 cut(s) 279, 1032
DpnII GATC 2 cut(s) 277, 1030
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 20, 69, 651
Eco24I GRGCYC 1 cut(s) 645
Eco57I CTGAAG 1 cut(s) 894
EcoNI CCTNNNNNAGG 1 cut(s) 670
EcoRI GAATTC 1 cut(s) 734
EcoRII CCWGG 3 cut(s) 561, 570, 784
EcoT14I CCWWGG 3 cut(s) 20, 69, 651
EcoT22I ATGCAT 1 cut(s) 11
EcoT38I GRGCYC 1 cut(s) 645
ErhI CCWWGG 3 cut(s) 20, 69, 651
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 591
FauNDI CATATG 2 cut(s) 163, 856
Fnu4HI GCNGC 1 cut(s) 844
FokI GGATG 2 cut(s) 969, 986
FriOI GRGCYC 1 cut(s) 645
Fsp4HI GCNGC 1 cut(s) 844
FspBI CTAG 1 cut(s) 1014
GluI GCNGC 1 cut(s) 844
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 2 cut(s) 438, 677
HapII CCGG 4 cut(s) 127, 224, 266, 532
HincII GTYRAC 2 cut(s) 33, 188
HindII GTYRAC 2 cut(s) 33, 188
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 9 cut(s) 34, 98, 110, 249, 523, 537, 618, 772, 1024
HpaII CCGG 4 cut(s) 127, 224, 266, 532
Hpy166II GTNNAC 2 cut(s) 33, 188
Hpy188I TCNGA 5 cut(s) 238, 298, 358, 623, 1005
Hpy188III TCNNGA 8 cut(s) 114, 142, 224, 595, 688, 698, 776, 1028
Hpy8I GTNNAC 2 cut(s) 33, 188
HpyAV CCTTC 2 cut(s) 192, 858
HpyCH4III ACNGT 3 cut(s) 106, 550, 611
HpyCH4IV ACGT 2 cut(s) 15, 360
HpyCH4V TGCA 5 cut(s) 9, 809, 921, 945, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 878
HpyF3I CTNAG 3 cut(s) 237, 585, 667
HpySE526I ACGT 2 cut(s) 15, 360
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 2 cut(s) 277, 1030
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 1 cut(s) 648
Lsp1109I GCAGC 1 cut(s) 855
LweI GCATC 3 cut(s) 55, 940, 964
MaeI CTAG 1 cut(s) 1014
MaeII ACGT 2 cut(s) 15, 360
MaeIII GTNAC 2 cut(s) 304, 911
MalI GATC 2 cut(s) 279, 1032
MboI GATC 2 cut(s) 277, 1030
MboII GAAGA 3 cut(s) 157, 843, 1025
MfeI CAATTG 1 cut(s) 1048
MflI RGATCY 2 cut(s) 277, 1030
MhlI GDGCHC 1 cut(s) 645
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 8 cut(s) 419, 456, 542, 682, 734, 795, 1040, 1048
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 5 cut(s) 28, 92, 243, 546, 627
MmeI TCCRAC 1 cut(s) 277
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 1 cut(s) 11
MreI CGCCGGCG 1 cut(s) 531
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 202, 628
MscI TGGCCA 1 cut(s) 438
MslI CAYNNNNRTG 3 cut(s) 86, 702, 940
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 4 cut(s) 127, 224, 266, 532
MspR9I CCNGG 3 cut(s) 563, 572, 786
MunI CAATTG 1 cut(s) 1048
MvaI CCWGG 3 cut(s) 563, 572, 786
MwoI GCNNNNNNNGC 2 cut(s) 166, 878
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 2 cut(s) 20, 651
NdeI CATATG 2 cut(s) 163, 856
NdeII GATC 2 cut(s) 277, 1030
NgoMIV GCCGGC 1 cut(s) 531
NheI GCTAGC 1 cut(s) 1013
NlaIV GGNNCC 4 cut(s) 75, 279, 493, 644
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 11
NspI RCATGY 4 cut(s) 9, 13, 885, 945
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 3 cut(s) 594, 687, 697
PciSI GCTCTTC 1 cut(s) 140
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 2 cut(s) 202, 628
PfeI GAWTC 4 cut(s) 110, 523, 772, 1024
PkrI GCNGC 1 cut(s) 845
PleI GAGTC 5 cut(s) 28, 92, 243, 545, 626
PpsI GAGTC 5 cut(s) 28, 92, 243, 545, 626
Psp6I CCWGG 3 cut(s) 561, 570, 784
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 3 cut(s) 561, 570, 784
PspN4I GGNNCC 4 cut(s) 75, 279, 493, 644
PspPI GGNCC 1 cut(s) 675
PsuI RGATCY 2 cut(s) 277, 1030
RsaI GTAC 4 cut(s) 14, 363, 398, 410
RsaNI GTAC 4 cut(s) 13, 362, 397, 409
RseI CAYNNNNRTG 3 cut(s) 86, 702, 940
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 1 cut(s) 844
Sau3AI GATC 2 cut(s) 277, 1030
Sau96I GGNCC 1 cut(s) 675
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 5 cut(s) 28, 92, 243, 546, 627
ScrFI CCNGG 3 cut(s) 563, 572, 786
SduI GDGCHC 1 cut(s) 645
SfaNI GCATC 3 cut(s) 55, 940, 964
SgrAI CRCCGGYG 1 cut(s) 531
SmiMI CAYNNNNRTG 3 cut(s) 86, 702, 940
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SphI GCATGC 1 cut(s) 9
Sse9I AATT 8 cut(s) 419, 456, 542, 682, 734, 795, 1040, 1048
SspMI CTAG 1 cut(s) 1014
StyD4I CCNGG 3 cut(s) 561, 570, 784
StyI CCWWGG 3 cut(s) 20, 69, 651
TaaI ACNGT 3 cut(s) 106, 550, 611
TaiI ACGT 2 cut(s) 18, 363
TaqI TCGA 1 cut(s) 134
TasI AATT 8 cut(s) 419, 456, 542, 682, 734, 795, 1040, 1048
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 4 cut(s) 110, 523, 772, 1024
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 304
TseI GCWGC 1 cut(s) 843
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 6 cut(s) 220, 583, 676, 704, 843, 971
TspRI CASTG 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 670
XapI RAATTY 3 cut(s) 456, 682, 734
XceI RCATGY 4 cut(s) 9, 13, 885, 945
XmnI GAANNNNTTC 2 cut(s) 202, 628
XspI CTAG 1 cut(s) 1014
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.