Rh2DG551300

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0004903
Physical Location & Seq
Forward (+)
77979667 .. 77987889
8223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG551300.1

Sequence Viewer

Length: 1071 bp
ATGGCATACATATACATTCCATGGCTCTTGTTCTCTCAAATCCTCCTACAAGTAGCAAAACATGAGGCCAAGGTTCCGGCTCTCATTGTCTTCGGAGACTCCACCGTGGATTCCGGAAACAATAACAGGATTAAAACACTTTTGAAGAGCAACTTCAAGCCCTATGGCCGCGACTTTGCTGGGGGTCAACCCACAGGAAGGTTCTCCAATGGTCGTGTTCCTCCGGATTTCATTTCTGAGGCTTTTGGACTGAAACCATTCGTTCCGGCTTACTTGGATCCCAACTATGGGATTTCGGATTTTGTTACTGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGACAATGCAACTTCTGATGTACTGAATGTGATCCCTCTATGGAAAGAAGTGGAGTACTACAAGGAGTACCAGGCCAAATTGAGAGCCCATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAAGCTTTGTATGTGATAAGCTTGGGAACCAATGATTTCCTCGAGAACTACTACTTGCTACCCAGCCGGCAAGTCCAATATACAGTCCAGCAGTACCAGGACTTCCTTGTTGGACTAGCCGAAAACTTCATGAGGGAGCTTTACAGTCTTGGAGCCAGGAAGATTTCCCTAACTGGGCTCCCTCCCATGGGGTGCTTGCCCTTAGAGAGAGCCATAAATTTCATGGGTCACCATGATTGTGTGGAGGAGTACAACAATGTGGCCGTGGAGTTTAATGTGAAGTTGAACAACATGGTGGCAAAGCTTAACCAAGTGCTTCCAGGCTATAGAGTTCTGTTTACAGAGAAGGTCTATCATCCGATATTTATACATGTGCGTGGCTTCATCACCGCTCTTACTAAAAGAGAGTCTTCGTCAGAGTACGTCTCACGCGTTAAGGTTGAGTTTCACAAGAAAGACGACATCGCTGCTAAAGTCATCGCCGGAGCCTTGAAACCCGATTTGGGAGCGGATCGATTGGGAGTCGAGGGCGGCGTTGTCGGGTTCTATTCCAGCGCGTTAATATGGAAGAAGTTGATGAGGTTGTCGACGATTGGAAGTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

356

Amino Acids

39.95

Weight (kDa)

8.85

Isoelectric Point (pI)

21.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 265 5.3e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 857, 974
AccI GTMKAC 1 cut(s) 1052
AccII CGCG 3 cut(s) 171, 897, 1022
AccIII TCCGGA 2 cut(s) 113, 223
AciI CCGC 4 cut(s) 169, 855, 974, 996
AclWI GGATC 4 cut(s) 272, 285, 367, 984
AcoI YGGCCR 3 cut(s) 166, 436, 726
AcsI RAATTY 2 cut(s) 456, 682
AfaI GTAC 6 cut(s) 363, 398, 410, 560, 716, 887
AfiI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 968
AflIII ACRYGT 2 cut(s) 835, 895
AgsI TTSAA 4 cut(s) 145, 157, 751, 958
AjnI CCWGG 4 cut(s) 411, 561, 620, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 950, 982
AluBI AGCT 4 cut(s) 470, 486, 604, 769
AluI AGCT 4 cut(s) 470, 486, 604, 769
Alw26I GTCTC 2 cut(s) 90, 895
AlwI GGATC 4 cut(s) 272, 285, 367, 984
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 2 cut(s) 113, 223
AoxI GGCC 5 cut(s) 66, 166, 414, 436, 726
ApeKI GCWGC 1 cut(s) 932
ApoI RAATTY 2 cut(s) 456, 682
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 1022
AsuHPI GGTGA 2 cut(s) 686, 844
AvaI CYCGRG 1 cut(s) 506
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 430, 645
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 2 cut(s) 82, 867
BbvI GCAGC 1 cut(s) 919
BccI CCATC 1 cut(s) 447
BceAI ACGGC 1 cut(s) 713
BcgI CGANNNNNNTGC 2 cut(s) 914, 948
BciT130I CCWGG 4 cut(s) 413, 563, 622, 786
BcoDI GTCTC 2 cut(s) 90, 895
BfaI CTAG 2 cut(s) 581, 1069
BfmI CTRYAG 1 cut(s) 790
BisI GCNGC 3 cut(s) 169, 933, 997
BlsI GCNGC 3 cut(s) 170, 934, 998
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 4 cut(s) 413, 563, 622, 786
BmeT110I CYCGRG 1 cut(s) 506
BmiI GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 952
BmrFI CCNGG 4 cut(s) 413, 563, 622, 786
BmrI ACTGGG 1 cut(s) 648
BmuI ACTGGG 1 cut(s) 648
BpiI GAAGAC 2 cut(s) 82, 867
BplI GAGNNNNNCTC 2 cut(s) 875, 907
Bsa29I ATCGAT 1 cut(s) 979
BsaJI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BsaWI WCCGGW 2 cut(s) 113, 223
Bsc4I CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 968
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 3 cut(s) 313, 340, 643
BseAI TCCGGA 2 cut(s) 113, 223
BseBI CCWGG 4 cut(s) 413, 563, 622, 786
BseCI ATCGAT 1 cut(s) 979
BseDI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BseGI GGATG 1 cut(s) 820
BseLI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 968
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 3 cut(s) 313, 340, 643
BseRI GAGGAG 1 cut(s) 725
BseXI GCAGC 1 cut(s) 919
BseYI CCCAGC 2 cut(s) 179, 527
Bsh1236I CGCG 3 cut(s) 171, 897, 1022
BshFI GGCC 5 cut(s) 68, 168, 416, 438, 728
BshVI ATCGAT 1 cut(s) 979
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 6 cut(s) 77, 114, 224, 266, 532, 948
BslI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 968
BsmAI GTCTC 2 cut(s) 90, 895
BsmBI CGTCTC 1 cut(s) 895
BsnI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 430, 645
Bsp13I TCCGGA 2 cut(s) 113, 223
Bsp143I GATC 3 cut(s) 277, 372, 976
Bsp19I CCATGG 2 cut(s) 20, 651
BspACI CCGC 4 cut(s) 169, 855, 974, 996
BspANI GGCC 5 cut(s) 68, 168, 416, 438, 728
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 979
BspEI TCCGGA 2 cut(s) 113, 223
BspFNI CGCG 3 cut(s) 171, 897, 1022
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 952
BspPI GGATC 4 cut(s) 272, 285, 367, 984
BspQI GCTCTTC 1 cut(s) 140
BsrBI CCGCTC 2 cut(s) 857, 974
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 3 cut(s) 313, 340, 643
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BssMI GATC 3 cut(s) 277, 372, 976
BssT1I CCWWGG 3 cut(s) 20, 69, 651
Bst2UI CCWGG 4 cut(s) 413, 563, 622, 786
Bst4CI ACNGT 3 cut(s) 106, 550, 611
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 533, 662
BstDEI CTNAG 2 cut(s) 237, 667
BstDSI CCRYGG 4 cut(s) 20, 105, 651, 729
BstEII GGTNACC 1 cut(s) 692
BstF5I GGATG 1 cut(s) 820
BstFNI CGCG 3 cut(s) 171, 897, 1022
BstHHI GCGC 1 cut(s) 1022
BstKTI GATC 3 cut(s) 280, 375, 979
BstMAI GTCTC 2 cut(s) 90, 895
BstMBI GATC 3 cut(s) 277, 372, 976
BstNI CCWGG 4 cut(s) 413, 563, 622, 786
BstNSI RCATGY 1 cut(s) 839
BstPI GGTNACC 1 cut(s) 692
BstSCI CCNGG 4 cut(s) 411, 561, 620, 784
BstSFI CTRYAG 1 cut(s) 790
BstUI CGCG 3 cut(s) 171, 897, 1022
BstV1I GCAGC 1 cut(s) 919
BstV2I GAAGAC 2 cut(s) 82, 867
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 979
BsuRI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsuTUI ATCGAT 1 cut(s) 979
BtgI CCRYGG 4 cut(s) 20, 105, 651, 729
BtgZI GCGATG 2 cut(s) 913, 928
BtsCI GGATG 1 cut(s) 820
Cac8I GCNNGC 2 cut(s) 533, 662
CciI TCATGA 1 cut(s) 594
CfoI GCGC 1 cut(s) 1022
Cfr10I RCCGGY 1 cut(s) 531
ClaI ATCGAT 1 cut(s) 979
Csp6I GTAC 6 cut(s) 362, 397, 409, 559, 715, 886
CviAII CATG 9 cut(s) 21, 62, 431, 595, 652, 688, 698, 757, 836
CviQI GTAC 6 cut(s) 362, 397, 409, 559, 715, 886
DdeI CTNAG 2 cut(s) 237, 667
DpnI GATC 3 cut(s) 279, 374, 978
DpnII GATC 3 cut(s) 277, 372, 976
EaeI YGGCCR 3 cut(s) 166, 436, 726
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 20, 69, 651
Eco24I GRGCYC 2 cut(s) 430, 645
Eco88I CYCGRG 1 cut(s) 506
Eco91I GGTNACC 1 cut(s) 692
EcoO65I GGTNACC 1 cut(s) 692
EcoRII CCWGG 4 cut(s) 411, 561, 620, 784
EcoT14I CCWWGG 3 cut(s) 20, 69, 651
EcoT38I GRGCYC 2 cut(s) 430, 645
ErhI CCWWGG 3 cut(s) 20, 69, 651
Esp3I CGTCTC 1 cut(s) 895
FaeI CATG 9 cut(s) 24, 65, 434, 598, 655, 691, 701, 760, 839
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 859, 891
FatI CATG 9 cut(s) 20, 61, 430, 594, 651, 687, 697, 756, 835
FblI GTMKAC 1 cut(s) 1052
Fnu4HI GCNGC 3 cut(s) 169, 933, 997
FokI GGATG 1 cut(s) 807
FriOI GRGCYC 2 cut(s) 430, 645
Fsp4HI GCNGC 3 cut(s) 169, 933, 997
FspBI CTAG 2 cut(s) 581, 1069
GlaI GCGC 1 cut(s) 1021
GluI GCNGC 3 cut(s) 169, 933, 997
GsaI CCCAGC 2 cut(s) 183, 531
HaeIII GGCC 5 cut(s) 68, 168, 416, 438, 728
HapII CCGG 6 cut(s) 77, 114, 224, 266, 532, 948
HhaI GCGC 1 cut(s) 1022
Hin1II CATG 9 cut(s) 24, 65, 434, 598, 655, 691, 701, 760, 839
Hin6I GCGC 1 cut(s) 1020
HinP1I GCGC 1 cut(s) 1020
HincII GTYRAC 2 cut(s) 188, 1053
HindII GTYRAC 2 cut(s) 188, 1053
HindIII AAGCTT 3 cut(s) 468, 484, 767
HinfI GANTC 4 cut(s) 98, 110, 872, 987
HpaII CCGG 6 cut(s) 77, 114, 224, 266, 532, 948
HphI GGTGA 2 cut(s) 686, 844
Hpy166II GTNNAC 3 cut(s) 188, 804, 1053
Hpy188I TCNGA 6 cut(s) 95, 238, 298, 358, 825, 883
Hpy188III TCNNGA 4 cut(s) 114, 224, 508, 595
Hpy8I GTNNAC 3 cut(s) 188, 804, 1053
Hpy99I CGWCG 1 cut(s) 1057
HpyAV CCTTC 2 cut(s) 192, 805
HpyCH4III ACNGT 3 cut(s) 106, 550, 611
HpyCH4IV ACGT 1 cut(s) 888
HpyCH4V TGCA 1 cut(s) 350
HpyF3I CTNAG 2 cut(s) 237, 667
HpySE526I ACGT 1 cut(s) 888
Hsp92II CATG 9 cut(s) 24, 65, 434, 598, 655, 691, 701, 760, 839
HspAI GCGC 1 cut(s) 1020
Kpn2I TCCGGA 2 cut(s) 113, 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 3 cut(s) 277, 372, 976
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 5 cut(s) 601, 617, 648, 950, 971
Lsp1109I GCAGC 1 cut(s) 919
MaeI CTAG 2 cut(s) 581, 1069
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 2 cut(s) 304, 692
MalI GATC 3 cut(s) 279, 374, 978
MbiI CCGCTC 2 cut(s) 857, 974
MboI GATC 3 cut(s) 277, 372, 976
MboII GAAGA 5 cut(s) 82, 157, 637, 867, 1045
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 430, 645
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 3 cut(s) 419, 456, 682
MluI ACGCGT 1 cut(s) 895
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 3 cut(s) 92, 881, 996
MmeI TCCRAC 1 cut(s) 556
Mox20I TGGCCA 1 cut(s) 438
MroI TCCGGA 2 cut(s) 113, 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 257
MscI TGGCCA 1 cut(s) 438
MseI TTAA 5 cut(s) 132, 738, 771, 900, 1025
MslI CAYNNNNRTG 2 cut(s) 702, 840
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 6 cut(s) 77, 114, 224, 266, 532, 948
MspR9I CCNGG 4 cut(s) 413, 563, 622, 786
MvaI CCWGG 4 cut(s) 413, 563, 622, 786
MvnI CGCG 3 cut(s) 171, 897, 1022
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 2 cut(s) 20, 651
NdeII GATC 3 cut(s) 277, 372, 976
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 9 cut(s) 24, 65, 434, 598, 655, 691, 701, 760, 839
NlaIV GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 952
NmuCI GTSAC 1 cut(s) 692
NspI RCATGY 1 cut(s) 839
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 594
PciI ACATGT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 2 cut(s) 894, 996
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 3 cut(s) 170, 934, 998
PleI GAGTC 3 cut(s) 92, 880, 995
PpsI GAGTC 3 cut(s) 92, 880, 995
PscI ACATGT 1 cut(s) 835
Psp6I CCWGG 4 cut(s) 411, 561, 620, 784
PspEI GGTNACC 1 cut(s) 692
PspFI CCCAGC 2 cut(s) 179, 527
PspGI CCWGG 4 cut(s) 411, 561, 620, 784
PspN4I GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 952
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 6 cut(s) 363, 398, 410, 560, 716, 887
RsaNI GTAC 6 cut(s) 362, 397, 409, 559, 715, 886
RseI CAYNNNNRTG 2 cut(s) 702, 840
SalI GTCGAC 1 cut(s) 1051
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 5 cut(s) 132, 738, 771, 900, 1025
SatI GCNGC 3 cut(s) 169, 933, 997
Sau3AI GATC 3 cut(s) 277, 372, 976
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 92, 881, 996
ScrFI CCNGG 4 cut(s) 413, 563, 622, 786
SduI GDGCHC 2 cut(s) 430, 645
SfcI CTRYAG 1 cut(s) 790
Sfr274I CTCGAG 1 cut(s) 506
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 702, 840
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 3 cut(s) 419, 456, 682
SsiI CCGC 4 cut(s) 169, 855, 974, 996
SspMI CTAG 2 cut(s) 581, 1069
StyD4I CCNGG 4 cut(s) 411, 561, 620, 784
StyI CCWWGG 3 cut(s) 20, 69, 651
TaaI ACNGT 3 cut(s) 106, 550, 611
TaiI ACGT 1 cut(s) 891
TaqI TCGA 4 cut(s) 507, 979, 990, 1052
TasI AATT 3 cut(s) 419, 456, 682
TatI WGTACW 3 cut(s) 361, 396, 714
TauI GCSGC 2 cut(s) 171, 999
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 5 cut(s) 132, 738, 771, 900, 1025
Tru9I TTAA 5 cut(s) 132, 738, 771, 900, 1025
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 1 cut(s) 932
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 4 cut(s) 220, 583, 676, 838
XapI RAATTY 2 cut(s) 456, 682
XceI RCATGY 1 cut(s) 839
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XhoI CTCGAG 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 1052
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 2 cut(s) 581, 1069
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.