Rroxscaffold_2G00092000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004903
Physical Location & Seq
Reverse (-)
13559657 .. 13562390
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00092000.1

Sequence Viewer

Length: 1053 bp
ATGGCATGCATATACGTTCCATGGCTCTTGGTGACTCAAATTCTACTACAAGTAGCAAAACATGAGGCCAAGGTTCCAGCCATTATTGTGTTTGGAGACTCGTCTGTGGATTCAGGAAACAACAACCAGATTTCGACACCCTTGAAGAGCAATTTCAAGCCATATGGGCGCGATTTTGCTGGCGGTCAACCCACAGGAAGGTTCTCCAATGGTCGTGTTCCTCCGGATTTCATCTCTGAGGCTTTCGGGCTGAAACCCTCTGTTCCTGCTTACTTGGATCCCAAGTTTGGGATTTCGGATTTTGTCACTGGTGTTTGCTTTGCTTCTGCTGGAACCGGCTATGACAATGCTACTTCTGATGTACTAAATGTTATACCTCTGTGGAAAGAAGTGGAGTACTACAAGGAGTACCAAAGCAAATTGAGAGCCTATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAGGCTTTGTACTTGATAAGCTTGGGAACCAATGACTTCCTTGAGAACTACTACACGATTCCTCGCCGGCGAGCCCAATTCACAGTCCAGCAGTACCAGGATTTCCTGGTGGAACTTGCTGAAAATTTCCTGAGGGAAATTTACAGTCTTGGCGTCAGAAAAATTTCCTTAGCTGGGCCACCTCCTATGGGGTGCTTGCCTTTAGAGAGGGCCACAAATTTCATGAATAATCATGCTTGTGTGGAGGAATATAACAACGTGGCTGTGGAATTTAATGGGAAGTTGAACAACATGGTAGCAAAACTAAATCAGGAGCTTCCAGGCTATAAAATTCTATTTACAGCAAAAGTATATCAGCTCTTCTATCAAATCATTAGAAGCCCTTCTTCATATGGATTTGATGTTGCTGAAGTGGCATGTTGTTCTACTGGGACATTTGAGATGAGTTACCTCTGCAACGAGGATAATCCATTTACATGCACAGATGCAAATAAGTATATATTTTGGGATGCCTTTCATCCTACAGACAAAACAAATCAGATAATAGCTAATTATTTGATTCCAGATCTTCTTAAATTATTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.46

Weight (kDa)

5.63

Isoelectric Point (pI)

32.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 341 1.9e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 1 cut(s) 183
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 8 cut(s) 39, 456, 589, 603, 627, 682, 734, 795
AcuI CTGAAG 1 cut(s) 894
AcyI GRCGYC 1 cut(s) 618
AfaI GTAC 5 cut(s) 363, 398, 410, 476, 560
AfiI CCNNNNNNNGG 6 cut(s) 198, 287, 288, 445, 639, 653
AgsI TTSAA 4 cut(s) 145, 157, 751, 1048
AjnI CCWGG 3 cut(s) 561, 570, 784
AluBI AGCT 5 cut(s) 486, 638, 781, 823, 1013
AluI AGCT 5 cut(s) 486, 638, 781, 823, 1013
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 272, 285
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 4 cut(s) 66, 436, 641, 675
ApoI RAATTY 8 cut(s) 39, 456, 589, 603, 627, 682, 734, 795
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 2 cut(s) 628, 847
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 2 cut(s) 641, 675
AsuHPI GGTGA 1 cut(s) 43
AxyI CCTNAGG 1 cut(s) 596
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 541
BccI CCATC 1 cut(s) 447
BciT130I CCWGG 3 cut(s) 563, 572, 786
BcoDI GTCTC 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 987
BglI GCCNNNNNGGC 1 cut(s) 166
BglII AGATCT 1 cut(s) 1030
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 563, 572, 786
BmgT120I GGNCC 2 cut(s) 641, 675
BmiI GGNNCC 4 cut(s) 75, 279, 334, 493
BmrFI CCNGG 3 cut(s) 563, 572, 786
BmrI ACTGGG 1 cut(s) 903
BmsI GCATC 2 cut(s) 940, 964
BmuI ACTGGG 1 cut(s) 903
Bpu10I CCTNAGC 1 cut(s) 634
BpuEI CTTGAG 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 20, 69
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 6 cut(s) 198, 287, 288, 445, 639, 653
Bse118I RCCGGY 2 cut(s) 335, 531
Bse1I ACTGG 2 cut(s) 313, 898
Bse21I CCTNAGG 1 cut(s) 596
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 3 cut(s) 563, 572, 786
BseDI CCNNGG 2 cut(s) 20, 69
BseGI GGATG 2 cut(s) 979, 982
BseLI CCNNNNNNNGG 6 cut(s) 198, 287, 288, 445, 639, 653
BseMII CTCAG 2 cut(s) 228, 587
BseNI ACTGG 2 cut(s) 313, 898
BseYI CCCAGC 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 4 cut(s) 68, 438, 643, 677
BsiSI CCGG 3 cut(s) 224, 336, 532
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 6 cut(s) 198, 287, 288, 445, 639, 653
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 910
BsnI GGCC 4 cut(s) 68, 438, 643, 677
Bsp1286I GDGCHC 1 cut(s) 541
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 2 cut(s) 277, 1030
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 1 cut(s) 183
BspANI GGCC 4 cut(s) 68, 438, 643, 677
BspCNI CTCAG 2 cut(s) 229, 588
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 4 cut(s) 75, 279, 334, 493
BspPI GGATC 2 cut(s) 272, 285
BspQI GCTCTTC 2 cut(s) 140, 830
BsrFI RCCGGY 2 cut(s) 335, 531
BsrI ACTGG 2 cut(s) 313, 898
BssAI RCCGGY 2 cut(s) 335, 531
BssECI CCNNGG 2 cut(s) 20, 69
BssMI GATC 2 cut(s) 277, 1030
BssNI GRCGYC 1 cut(s) 618
BssT1I CCWWGG 2 cut(s) 20, 69
Bst2UI CCWGG 3 cut(s) 563, 572, 786
Bst4CI ACNGT 2 cut(s) 550, 611
Bst6I CTCTTC 2 cut(s) 140, 830
BstACI GRCGYC 1 cut(s) 618
BstC8I GCNNGC 5 cut(s) 7, 181, 533, 537, 662
BstDEI CTNAG 3 cut(s) 237, 596, 634
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 979, 982
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 280, 1033
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 2 cut(s) 277, 1030
BstMWI GCNNNNNNNGC 2 cut(s) 166, 878
BstNI CCWGG 3 cut(s) 563, 572, 786
BstNSI RCATGY 3 cut(s) 9, 885, 945
BstSCI CCNGG 3 cut(s) 561, 570, 784
BstSFI CTRYAG 1 cut(s) 987
BstUI CGCG 1 cut(s) 171
BstX2I RGATCY 2 cut(s) 277, 1030
BstYI RGATCY 2 cut(s) 277, 1030
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 4 cut(s) 68, 438, 643, 677
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 979, 982
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 5 cut(s) 7, 181, 533, 537, 662
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 2 cut(s) 335, 531
Cfr13I GGNCC 2 cut(s) 641, 675
CseI GACGC 1 cut(s) 607
Csp6I GTAC 5 cut(s) 362, 397, 409, 475, 559
CviAII CATG 8 cut(s) 6, 21, 62, 688, 698, 757, 882, 942
CviQI GTAC 5 cut(s) 362, 397, 409, 475, 559
DdeI CTNAG 3 cut(s) 237, 596, 634
DpnI GATC 2 cut(s) 279, 1032
DpnII GATC 2 cut(s) 277, 1030
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 2 cut(s) 140, 830
EarI CTCTTC 2 cut(s) 140, 830
Eco130I CCWWGG 2 cut(s) 20, 69
Eco24I GRGCYC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 894
Eco81I CCTNAGG 1 cut(s) 596
EcoRII CCWGG 3 cut(s) 561, 570, 784
EcoT14I CCWWGG 2 cut(s) 20, 69
EcoT22I ATGCAT 1 cut(s) 11
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 2 cut(s) 20, 69
FaeI CATG 8 cut(s) 9, 24, 65, 691, 701, 760, 885, 945
FalI AAGNNNNNCTT 2 cut(s) 835, 867
FaqI GGGAC 1 cut(s) 910
FatI CATG 8 cut(s) 5, 20, 61, 687, 697, 756, 881, 941
FauNDI CATATG 2 cut(s) 163, 856
FokI GGATG 2 cut(s) 969, 986
FriOI GRGCYC 1 cut(s) 541
GlaI GCGC 1 cut(s) 170
GsaI CCCAGC 1 cut(s) 642
HaeIII GGCC 4 cut(s) 68, 438, 643, 677
HapII CCGG 3 cut(s) 224, 336, 532
HgaI GACGC 1 cut(s) 607
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 1 cut(s) 618
Hin1II CATG 8 cut(s) 9, 24, 65, 691, 701, 760, 885, 945
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 188
HindII GTYRAC 1 cut(s) 188
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 5 cut(s) 34, 98, 110, 523, 1024
HpaII CCGG 3 cut(s) 224, 336, 532
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 1 cut(s) 188
Hpy188I TCNGA 5 cut(s) 238, 298, 358, 623, 1005
Hpy188III TCNNGA 6 cut(s) 114, 224, 595, 688, 776, 1028
Hpy8I GTNNAC 1 cut(s) 188
HpyAV CCTTC 2 cut(s) 192, 858
HpyCH4III ACNGT 2 cut(s) 550, 611
HpyCH4IV ACGT 2 cut(s) 15, 723
HpyCH4V TGCA 4 cut(s) 9, 921, 945, 953
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 878
HpyF3I CTNAG 3 cut(s) 237, 596, 634
HpySE526I ACGT 2 cut(s) 15, 723
Hsp92I GRCGYC 1 cut(s) 618
Hsp92II CATG 8 cut(s) 9, 24, 65, 691, 701, 760, 885, 945
HspAI GCGC 1 cut(s) 169
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 2 cut(s) 277, 1030
LguI GCTCTTC 2 cut(s) 140, 830
LmnI GCTCC 1 cut(s) 778
LweI GCATC 2 cut(s) 940, 964
MaeII ACGT 2 cut(s) 15, 723
MaeIII GTNAC 3 cut(s) 31, 304, 911
MalI GATC 2 cut(s) 279, 1032
MboI GATC 2 cut(s) 277, 1030
MboII GAAGA 4 cut(s) 157, 817, 843, 1025
MfeI CAATTG 1 cut(s) 1048
MflI RGATCY 2 cut(s) 277, 1030
MhlI GDGCHC 1 cut(s) 541
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 2 cut(s) 28, 92
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 1 cut(s) 11
MreI CGCCGGCG 1 cut(s) 531
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 628, 847
MscI TGGCCA 1 cut(s) 438
MseI TTAA 2 cut(s) 738, 1038
MslI CAYNNNNRTG 3 cut(s) 86, 702, 940
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 3 cut(s) 224, 336, 532
MspR9I CCNGG 3 cut(s) 563, 572, 786
MunI CAATTG 1 cut(s) 1048
MvaI CCWGG 3 cut(s) 563, 572, 786
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 166, 878
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 1 cut(s) 20
NdeI CATATG 2 cut(s) 163, 856
NdeII GATC 2 cut(s) 277, 1030
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 8 cut(s) 9, 24, 65, 691, 701, 760, 885, 945
NlaIV GGNNCC 4 cut(s) 75, 279, 334, 493
NmuCI GTSAC 2 cut(s) 31, 304
NsiI ATGCAT 1 cut(s) 11
NspI RCATGY 3 cut(s) 9, 885, 945
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 1 cut(s) 687
PciSI GCTCTTC 2 cut(s) 140, 830
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 2 cut(s) 628, 847
PfeI GAWTC 3 cut(s) 110, 523, 1024
PleI GAGTC 2 cut(s) 28, 92
PpsI GAGTC 2 cut(s) 28, 92
Psp6I CCWGG 3 cut(s) 561, 570, 784
PspFI CCCAGC 1 cut(s) 638
PspGI CCWGG 3 cut(s) 561, 570, 784
PspN4I GGNNCC 4 cut(s) 75, 279, 334, 493
PspPI GGNCC 2 cut(s) 641, 675
PsuI RGATCY 2 cut(s) 277, 1030
RsaI GTAC 5 cut(s) 363, 398, 410, 476, 560
RsaNI GTAC 5 cut(s) 362, 397, 409, 475, 559
RseI CAYNNNNRTG 3 cut(s) 86, 702, 940
SapI GCTCTTC 2 cut(s) 140, 830
SaqAI TTAA 2 cut(s) 738, 1038
Sau3AI GATC 2 cut(s) 277, 1030
Sau96I GGNCC 2 cut(s) 641, 675
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 28, 92
ScrFI CCNGG 3 cut(s) 563, 572, 786
SduI GDGCHC 1 cut(s) 541
SfaNI GCATC 2 cut(s) 940, 964
SfcI CTRYAG 1 cut(s) 987
SgrAI CRCCGGYG 1 cut(s) 531
SmiMI CAYNNNNRTG 3 cut(s) 86, 702, 940
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SphI GCATGC 1 cut(s) 9
SsiI CCGC 1 cut(s) 183
StyD4I CCNGG 3 cut(s) 561, 570, 784
StyI CCWWGG 2 cut(s) 20, 69
TaaI ACNGT 2 cut(s) 550, 611
TaiI ACGT 2 cut(s) 18, 726
TaqI TCGA 1 cut(s) 134
TatI WGTACW 3 cut(s) 361, 396, 474
TfiI GAWTC 3 cut(s) 110, 523, 1024
Tru1I TTAA 2 cut(s) 738, 1038
Tru9I TTAA 2 cut(s) 738, 1038
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 2 cut(s) 31, 304
Tsp45I GTSAC 2 cut(s) 31, 304
TspDTI ATGAA 5 cut(s) 220, 676, 704, 843, 971
TspRI CASTG 1 cut(s) 313
XapI RAATTY 8 cut(s) 39, 456, 589, 603, 627, 682, 734, 795
XceI RCATGY 3 cut(s) 9, 885, 945
XmnI GAANNNNTTC 2 cut(s) 628, 847
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.