MD09G1116600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004903
Physical Location & Seq
Reverse (-)
8974229 .. 8977015
2787 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1116600.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCATTCGTATACATTTCATGGTTTCTTTTAACTCAAATCCTGCTTCAAGTTGCAAAACATGAGGCTAAAGTTCCAGCAGTTATTGTGTTTGGGGACTCAACTGTGGACTCTGGGAACAACAATGGGATTTCCACACTTCTGAAGAGCAACTTCAAGCCGTATGGCCGTGACTTTGACGGTGGTCAGCCGACAGGGAGGTTCTCCAATGGCCGAGTTCCACCGGATTTTGTTTCCGAGGCTTTCGGGCTCAAAACTTCGGTTCCTGCATACTTGGACCCCAAGTTTGGGATTTCAGATTTTGTCAATGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGATAATGCAACTTCTGATGTACTAAATGTGATACCTCTGTGGAAAGAAGTGGAGTACTACAAGGAGTACCAGAACAAACTCAAAGCCTATGTTGGCCATGACAAGGCAAAAGAGATTCTGACAGAGGCTTTATACTTGATAAGCTTAGGAACAAACGACTTCCTCGAGAACTACTACGTATTGCCCCGCCGGCGAATGCAATTCACAGTCCGGCAGTACGAGGATTTCCTCGTTGGACTTTCCGAAAACTTCATTAGGGAAATCTACAGTCTAGGGGTCAGGAAAATATCCCTAACCGGGCTTCCTCCGATGGGGTGTTTGCCATTAGAGAGAGCCACTAATTTCATGGCAAATAATGATTGTATGCAGGAATACAACAATGTGGCTGTGGAATTTAATGGGAAATTGAAAAATATGGTGGCAAAGCTGAATCAAGAGCTTCCAGGGCTTAGAATCCTATTGACAGAAAAATTATATCCGCTGTTCTACCAAATCATCAAAAGACCTTCATCATATGGATTTGAGTATGCTGAGAAGGCATGTTGTGCCACTGGGACATTCGAGATGAGTTACCTCTGTAATCAGGACAACCCATATACGTGCGCAGATGCAAATAAGTTCATATTTTGGGATGCCTTCCATCCAACGGAGAAAACAAATAAGATACTCGCAGACCATGTAATTGGCGATCTTTTAGACACCTTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

39.58

Weight (kDa)

5.54

Isoelectric Point (pI)

28.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 341 4.5e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 946
AccI GTMKAC 1 cut(s) 12
AciI CCGC 2 cut(s) 529, 821
AcoI YGGCCR 3 cut(s) 166, 211, 436
AcsI RAATTY 1 cut(s) 734
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 4 cut(s) 363, 398, 410, 560
AfiI CCNNNNNNNGG 6 cut(s) 287, 288, 445, 639, 653, 988
AgsI TTSAA 3 cut(s) 50, 157, 751
AjnI CCWGG 1 cut(s) 784
AluBI AGCT 3 cut(s) 486, 769, 781
AluI AGCT 3 cut(s) 486, 769, 781
Ama87I CYCGRG 1 cut(s) 506
AoxI GGCC 3 cut(s) 166, 211, 436
ApoI RAATTY 1 cut(s) 734
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AspLEI GCGC 1 cut(s) 947
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 640
AvaI CYCGRG 1 cut(s) 506
AvaII GGWCC 1 cut(s) 277
BaeI ACNNNNGTAYC 2 cut(s) 365, 398
BalI TGGCCA 1 cut(s) 438
BanII GRGCYC 1 cut(s) 252
BccI CCATC 2 cut(s) 646, 990
BceAI ACGGC 2 cut(s) 145, 153
BciT130I CCWGG 1 cut(s) 786
BcnI CCSGG 1 cut(s) 640
BfaI CTAG 1 cut(s) 614
BfmI CTRYAG 1 cut(s) 607
BglI GCCNNNNNGGC 1 cut(s) 532
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 640, 786
Bme18I GGWCC 1 cut(s) 277
BmeT110I CYCGRG 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 2 cut(s) 264, 279
BmrFI CCNGG 2 cut(s) 640, 786
BmrI ACTGGG 1 cut(s) 903
BmsI GCATC 2 cut(s) 940, 964
BmuI ACTGGG 1 cut(s) 903
BoxI GACNNNNGTC 1 cut(s) 183
Bpu10I CCTNAGC 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 640
BsaAI YACGTR 2 cut(s) 520, 942
BsaJI CCNNGG 2 cut(s) 237, 785
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 6 cut(s) 287, 288, 445, 639, 653, 988
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 2 cut(s) 340, 898
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 237, 785
BseGI GGATG 2 cut(s) 979, 982
BseLI CCNNNNNNNGG 6 cut(s) 287, 288, 445, 639, 653, 988
BseMII CTCAG 1 cut(s) 864
BseNI ACTGG 2 cut(s) 340, 898
BshFI GGCC 3 cut(s) 168, 213, 438
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 4 cut(s) 224, 532, 553, 639
BslFI GGGAC 2 cut(s) 110, 910
BslI CCNNNNNNNGG 6 cut(s) 287, 288, 445, 639, 653, 988
BsmFI GGGAC 2 cut(s) 110, 910
BsmI GAATGC 2 cut(s) 5, 543
BsnI GGCC 3 cut(s) 168, 213, 438
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 1030
BspACI CCGC 2 cut(s) 529, 821
BspANI GGCC 3 cut(s) 168, 213, 438
BspCNI CTCAG 1 cut(s) 865
BspLI GGNNCC 2 cut(s) 264, 279
BspQI GCTCTTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 2 cut(s) 340, 898
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 2 cut(s) 237, 785
BssMI GATC 1 cut(s) 1030
BssNAI GTATAC 1 cut(s) 13
Bst1107I GTATAC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 4 cut(s) 106, 182, 550, 611
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 887
BstBAI YACGTR 2 cut(s) 520, 942
BstC8I GCNNGC 1 cut(s) 533
BstDEI CTNAG 3 cut(s) 487, 791, 873
BstF5I GGATG 2 cut(s) 979, 982
BstHHI GCGC 1 cut(s) 947
BstKTI GATC 1 cut(s) 1033
BstMBI GATC 1 cut(s) 1030
BstMWI GCNNNNNNNGC 4 cut(s) 532, 787, 878, 887
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 1 cut(s) 885
BstPAI GACNNNNGTC 1 cut(s) 183
BstSCI CCNGG 2 cut(s) 638, 784
BstSFI CTRYAG 1 cut(s) 607
BstSNI TACGTA 1 cut(s) 520
BstXI CCANNNNNNTGG 1 cut(s) 1025
BstZ17I GTATAC 1 cut(s) 13
BsuRI GGCC 3 cut(s) 168, 213, 438
BtsCI GGATG 2 cut(s) 979, 982
BtsIMutI CAGTG 1 cut(s) 891
Cac8I GCNNGC 1 cut(s) 533
CfoI GCGC 1 cut(s) 947
Cfr10I RCCGGY 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 277
Csp6I GTAC 4 cut(s) 362, 397, 409, 559
CviAII CATG 6 cut(s) 21, 62, 440, 688, 882, 1019
CviQI GTAC 4 cut(s) 362, 397, 409, 559
DdeI CTNAG 3 cut(s) 487, 791, 873
DpnI GATC 1 cut(s) 1032
DpnII GATC 1 cut(s) 1030
EaeI YGGCCR 3 cut(s) 166, 211, 436
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco105I TACGTA 1 cut(s) 520
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 277
Eco57I CTGAAG 1 cut(s) 164
Eco88I CYCGRG 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 784
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 6 cut(s) 24, 65, 443, 691, 885, 1022
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 2 cut(s) 110, 910
FatI CATG 6 cut(s) 20, 61, 439, 687, 881, 1018
FauI CCCGC 1 cut(s) 536
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 12
FokI GGATG 2 cut(s) 969, 986
FriOI GRGCYC 1 cut(s) 252
FspBI CTAG 1 cut(s) 614
FspI TGCGCA 1 cut(s) 946
GlaI GCGC 1 cut(s) 946
HaeIII GGCC 3 cut(s) 168, 213, 438
HapII CCGG 4 cut(s) 224, 532, 553, 639
HhaI GCGC 1 cut(s) 947
Hin1II CATG 6 cut(s) 24, 65, 443, 691, 885, 1022
Hin6I GCGC 1 cut(s) 945
HinP1I GCGC 1 cut(s) 945
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 5 cut(s) 98, 110, 457, 772, 795
HpaII CCGG 4 cut(s) 224, 532, 553, 639
Hpy166II GTNNAC 2 cut(s) 13, 109
Hpy188I TCNGA 7 cut(s) 144, 238, 298, 358, 462, 586, 651
Hpy188III TCNNGA 5 cut(s) 508, 622, 776, 904, 926
Hpy8I GTNNAC 2 cut(s) 13, 109
HpyAV CCTTC 3 cut(s) 858, 871, 988
HpyCH4III ACNGT 4 cut(s) 106, 182, 550, 611
HpyCH4IV ACGT 2 cut(s) 519, 941
HpyCH4V TGCA 6 cut(s) 56, 269, 350, 541, 709, 953
HpyF10VI GCNNNNNNNGC 4 cut(s) 532, 787, 878, 887
HpyF3I CTNAG 3 cut(s) 487, 791, 873
HpySE526I ACGT 2 cut(s) 519, 941
Hsp92II CATG 6 cut(s) 24, 65, 443, 691, 885, 1022
HspAI GCGC 1 cut(s) 945
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 1 cut(s) 1030
LguI GCTCTTC 1 cut(s) 140
LweI GCATC 2 cut(s) 940, 964
MaeI CTAG 1 cut(s) 614
MaeII ACGT 2 cut(s) 519, 941
MaeIII GTNAC 2 cut(s) 170, 911
MalI GATC 1 cut(s) 1032
MboI GATC 1 cut(s) 1030
MboII GAAGA 1 cut(s) 157
MhlI GDGCHC 1 cut(s) 252
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 7 cut(s) 542, 682, 734, 746, 812, 1023, 1048
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 2 cut(s) 92, 104
MmeI TCCRAC 2 cut(s) 556, 1010
Mox20I TGGCCA 1 cut(s) 438
MreI CGCCGGCG 1 cut(s) 531
MroNI GCCGGC 1 cut(s) 531
MscI TGGCCA 1 cut(s) 438
MseI TTAA 3 cut(s) 32, 738, 1047
MslI CAYNNNNRTG 1 cut(s) 940
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 4 cut(s) 224, 532, 553, 639
MspR9I CCNGG 2 cut(s) 640, 786
Mva1269I GAATGC 2 cut(s) 5, 543
MvaI CCWGG 1 cut(s) 786
MwoI GCNNNNNNNGC 4 cut(s) 532, 787, 878, 887
NaeI GCCGGC 1 cut(s) 533
NciI CCSGG 1 cut(s) 640
NdeI CATATG 1 cut(s) 856
NdeII GATC 1 cut(s) 1030
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 6 cut(s) 24, 65, 443, 691, 885, 1022
NlaIV GGNNCC 2 cut(s) 264, 279
NmeAIII GCCGAG 1 cut(s) 239
NmuCI GTSAC 1 cut(s) 170
NsbI TGCGCA 1 cut(s) 946
NspI RCATGY 1 cut(s) 885
PaeR7I CTCGAG 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 504
PctI GAATGC 2 cut(s) 5, 543
PdiI GCCGGC 1 cut(s) 533
PfeI GAWTC 3 cut(s) 457, 772, 795
PleI GAGTC 2 cut(s) 92, 104
PpsI GAGTC 2 cut(s) 92, 104
Ppu21I YACGTR 2 cut(s) 520, 942
PshAI GACNNNNGTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 784
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 2 cut(s) 264, 279
PspPI GGNCC 1 cut(s) 277
RsaI GTAC 4 cut(s) 363, 398, 410, 560
RsaNI GTAC 4 cut(s) 362, 397, 409, 559
RseI CAYNNNNRTG 1 cut(s) 940
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 3 cut(s) 32, 738, 1047
Sau3AI GATC 1 cut(s) 1030
Sau96I GGNCC 1 cut(s) 277
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 92, 104
ScrFI CCNGG 2 cut(s) 640, 786
SduI GDGCHC 1 cut(s) 252
SfaNI GCATC 2 cut(s) 940, 964
SfcI CTRYAG 1 cut(s) 607
Sfr274I CTCGAG 1 cut(s) 506
SgrAI CRCCGGYG 1 cut(s) 531
SinI GGWCC 1 cut(s) 277
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 1 cut(s) 940
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SnaBI TACGTA 1 cut(s) 520
Sse9I AATT 7 cut(s) 542, 682, 734, 746, 812, 1023, 1048
SsiI CCGC 2 cut(s) 529, 821
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 638, 784
TaaI ACNGT 4 cut(s) 106, 182, 550, 611
TaiI ACGT 2 cut(s) 522, 944
TaqI TCGA 2 cut(s) 507, 903
TasI AATT 7 cut(s) 542, 682, 734, 746, 812, 1023, 1048
TatI WGTACW 2 cut(s) 361, 396
TfiI GAWTC 3 cut(s) 457, 772, 795
Tru1I TTAA 3 cut(s) 32, 738, 1047
Tru9I TTAA 3 cut(s) 32, 738, 1047
TscAI CASTG 1 cut(s) 898
TseFI GTSAC 1 cut(s) 170
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 5 cut(s) 9, 583, 676, 840, 952
TspGWI ACGGA 1 cut(s) 1004
TspRI CASTG 1 cut(s) 898
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 1 cut(s) 734
XceI RCATGY 1 cut(s) 885
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XhoI CTCGAG 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 1 cut(s) 614
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.