RH2AG528000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0004903
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG528000.1

Sequence Viewer

Length: 1149 bp
ATGGCATGCATATACGTTCCATGGCTCTTGTTGACTCAAATGCTACTACAAGTAGCAAAACATGAGGCCAAGGTTCCAGCCATTATTGTGTTTGGAGACTCGTCGGTGGATTCAGGAAACAACAACCAGATTTCGACACCCTTGAAGAGCAATTTCAAGCCATATGGGCGCGATTTTGCTGGCGGTCAACCCACAGGAAGGTTCTCCAATGGTCGTGTTCCTCCGGATCTCATCTCTGAGGCTTTCGGGCTGAAACCCTCTGTTCCTGCTTACTTGGATCCTAAGTTTGGGATTTCGGATTTTGTCACTGGTGTTTGCTTTGCTTCTGCTGGAACCGGCTATGACAATGCTACTTCTGATGTACTGAATGTTATACCTCTGTGGAAAGAAGTGGAGTACTACAAGGAGTACCAAAGCAAATTGAGAGCCTATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAGGCTTTGTACTTGATAAGCTTGGGAACCAATGACTTCCTTGAGAACTACTACACGATTCCTCGCCGGCGAGCCCAATTCACAGTCCAGCAGTACCAGGATTTCCTGGTGGAACTTGCTGAAAATTTCCTGAGGGAAATTTACAGTCTTGGAGTCAGAAAAATTTCCTTAACTGGGGCACCTCCTATGGGGTGCTTGCCCTTAGAGAGGGCCACAAATTTCATGAATAATCATGATTGTGTGGAGGAATATAACAACGTGGCTGTGGAATTTAATGGGAAGTTGAACAGCATGGTAGTAAAACTAAATCAGGAGCTTCCAGGCTATAAAATTCTATTTACAGCAAAAGTATATCAGCTCTTCTATCAAATCATTAGAAGCCCTTCTTCATATGGATTTGATGTTGCTGAAGTGGCATGTTGTTCTACTGGGACATTTGAGATGAGTTACCTCTGCAACGAGGATAATCCATTTACATGCACAGATGCAAGGTTTGATGTTGTTGAAGTGGGATGTTGTTCTACTGGGAGATTCGAGATGAGTTACCTCTGTAGCGAACCAAATCCATATACATGCAAAGATGCAAATAAGTATATGTTCTGGGATGCCTTCCATCCTACAGAAAAAACAAATAAGATAGTCTCCAACTATTTAATTCCAGATCTTCTACAATTCTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

382

Amino Acids

43.16

Weight (kDa)

5.29

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 372 5.8e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 643
AccII CGCG 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 1 cut(s) 183
AclWI GGATC 3 cut(s) 234, 272, 285
AcoI YGGCCR 1 cut(s) 436
AcsI RAATTY 7 cut(s) 456, 589, 603, 627, 682, 734, 795
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 5 cut(s) 363, 398, 410, 476, 560
AfiI CCNNNNNNNGG 6 cut(s) 198, 287, 445, 639, 653, 672
AgsI TTSAA 5 cut(s) 145, 157, 751, 971, 1144
AjnI CCWGG 3 cut(s) 561, 570, 784
AluBI AGCT 3 cut(s) 486, 781, 823
AluI AGCT 3 cut(s) 486, 781, 823
Alw26I GTCTC 2 cut(s) 90, 1111
AlwI GGATC 3 cut(s) 234, 272, 285
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 3 cut(s) 66, 436, 675
ApoI RAATTY 7 cut(s) 456, 589, 603, 627, 682, 734, 795
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 2 cut(s) 628, 847
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 675
AxyI CCTNAGG 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 646
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 643
BanII GRGCYC 1 cut(s) 541
BccI CCATC 2 cut(s) 447, 1086
BciT130I CCWGG 3 cut(s) 563, 572, 786
BcoDI GTCTC 2 cut(s) 90, 1111
BfmI CTRYAG 2 cut(s) 1015, 1083
BglI GCCNNNNNGGC 1 cut(s) 166
BglII AGATCT 1 cut(s) 1126
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 563, 572, 786
BmgT120I GGNCC 1 cut(s) 675
BmiI GGNNCC 5 cut(s) 75, 279, 334, 493, 645
BmrFI CCNGG 3 cut(s) 563, 572, 786
BmrI ACTGGG 3 cut(s) 648, 903, 999
BmsI GCATC 3 cut(s) 940, 1036, 1060
BmuI ACTGGG 3 cut(s) 648, 903, 999
BpuEI CTTGAG 1 cut(s) 527
BsaJI CCNNGG 2 cut(s) 20, 69
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 6 cut(s) 198, 287, 445, 639, 653, 672
Bse118I RCCGGY 2 cut(s) 335, 531
Bse1I ACTGG 4 cut(s) 313, 643, 898, 994
Bse21I CCTNAGG 1 cut(s) 596
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 3 cut(s) 563, 572, 786
BseDI CCNNGG 2 cut(s) 20, 69
BseGI GGATG 3 cut(s) 983, 1075, 1078
BseLI CCNNNNNNNGG 6 cut(s) 198, 287, 445, 639, 653, 672
BseMII CTCAG 2 cut(s) 228, 587
BseNI ACTGG 4 cut(s) 313, 643, 898, 994
BseSI GKGCMC 1 cut(s) 646
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 3 cut(s) 68, 438, 677
BshNI GGYRCC 1 cut(s) 643
BsiSI CCGG 3 cut(s) 224, 336, 532
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 6 cut(s) 198, 287, 445, 639, 653, 672
BsmAI GTCTC 2 cut(s) 90, 1111
BsmFI GGGAC 1 cut(s) 910
BsnI GGCC 3 cut(s) 68, 438, 677
Bsp1286I GDGCHC 2 cut(s) 541, 646
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 3 cut(s) 226, 277, 1126
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 1 cut(s) 183
BspANI GGCC 3 cut(s) 68, 438, 677
BspCNI CTCAG 2 cut(s) 229, 588
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 2 cut(s) 687, 697
BspLI GGNNCC 5 cut(s) 75, 279, 334, 493, 645
BspPI GGATC 3 cut(s) 234, 272, 285
BspQI GCTCTTC 2 cut(s) 140, 830
BspT107I GGYRCC 1 cut(s) 643
BsrFI RCCGGY 2 cut(s) 335, 531
BsrI ACTGG 4 cut(s) 313, 643, 898, 994
BssAI RCCGGY 2 cut(s) 335, 531
BssECI CCNNGG 2 cut(s) 20, 69
BssMI GATC 3 cut(s) 226, 277, 1126
BssT1I CCWWGG 2 cut(s) 20, 69
Bst2UI CCWGG 3 cut(s) 563, 572, 786
Bst4CI ACNGT 2 cut(s) 550, 611
Bst6I CTCTTC 2 cut(s) 140, 830
BstC8I GCNNGC 5 cut(s) 7, 181, 533, 537, 662
BstDEI CTNAG 4 cut(s) 237, 282, 596, 667
BstDSI CCRYGG 1 cut(s) 20
BstENI CCTNNNNNAGG 1 cut(s) 670
BstF5I GGATG 3 cut(s) 983, 1075, 1078
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 3 cut(s) 229, 280, 1129
BstMAI GTCTC 2 cut(s) 90, 1111
BstMBI GATC 3 cut(s) 226, 277, 1126
BstMWI GCNNNNNNNGC 2 cut(s) 166, 878
BstNI CCWGG 3 cut(s) 563, 572, 786
BstNSI RCATGY 4 cut(s) 9, 885, 945, 1041
BstSCI CCNGG 3 cut(s) 561, 570, 784
BstSFI CTRYAG 2 cut(s) 1015, 1083
BstSLI GKGCMC 1 cut(s) 646
BstUI CGCG 1 cut(s) 171
BstX2I RGATCY 3 cut(s) 226, 277, 1126
BstYI RGATCY 3 cut(s) 226, 277, 1126
Bsu36I CCTNAGG 1 cut(s) 596
BsuRI GGCC 3 cut(s) 68, 438, 677
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 3 cut(s) 983, 1075, 1078
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 5 cut(s) 7, 181, 533, 537, 662
CciI TCATGA 2 cut(s) 687, 697
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 2 cut(s) 335, 531
Cfr13I GGNCC 1 cut(s) 675
Csp6I GTAC 5 cut(s) 362, 397, 409, 475, 559
CviAII CATG 9 cut(s) 6, 21, 62, 688, 698, 757, 882, 942, 1038
CviQI GTAC 5 cut(s) 362, 397, 409, 475, 559
DdeI CTNAG 4 cut(s) 237, 282, 596, 667
DpnI GATC 3 cut(s) 228, 279, 1128
DpnII GATC 3 cut(s) 226, 277, 1126
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 2 cut(s) 140, 830
EarI CTCTTC 2 cut(s) 140, 830
Eco130I CCWWGG 2 cut(s) 20, 69
Eco24I GRGCYC 1 cut(s) 541
Eco57I CTGAAG 1 cut(s) 894
Eco81I CCTNAGG 1 cut(s) 596
EcoNI CCTNNNNNAGG 1 cut(s) 670
EcoRII CCWGG 3 cut(s) 561, 570, 784
EcoT14I CCWWGG 2 cut(s) 20, 69
EcoT22I ATGCAT 1 cut(s) 11
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 2 cut(s) 20, 69
FaeI CATG 9 cut(s) 9, 24, 65, 691, 701, 760, 885, 945, 1041
FalI AAGNNNNNCTT 2 cut(s) 835, 867
FaqI GGGAC 1 cut(s) 910
FatI CATG 9 cut(s) 5, 20, 61, 687, 697, 756, 881, 941, 1037
FauNDI CATATG 2 cut(s) 163, 856
FokI GGATG 3 cut(s) 990, 1065, 1082
FriOI GRGCYC 1 cut(s) 541
GlaI GCGC 1 cut(s) 170
HaeIII GGCC 3 cut(s) 68, 438, 677
HapII CCGG 3 cut(s) 224, 336, 532
HhaI GCGC 1 cut(s) 171
Hin1II CATG 9 cut(s) 9, 24, 65, 691, 701, 760, 885, 945, 1041
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 2 cut(s) 33, 188
HindII GTYRAC 2 cut(s) 33, 188
HindIII AAGCTT 1 cut(s) 484
HinfI GANTC 6 cut(s) 34, 98, 110, 523, 618, 996
HpaII CCGG 3 cut(s) 224, 336, 532
Hpy166II GTNNAC 2 cut(s) 33, 188
Hpy188I TCNGA 4 cut(s) 238, 298, 358, 623
Hpy188III TCNNGA 8 cut(s) 114, 224, 595, 688, 698, 776, 1000, 1124
Hpy8I GTNNAC 2 cut(s) 33, 188
Hpy99I CGWCG 1 cut(s) 106
HpyAV CCTTC 3 cut(s) 192, 858, 1084
HpyCH4III ACNGT 2 cut(s) 550, 611
HpyCH4IV ACGT 2 cut(s) 15, 723
HpyCH4V TGCA 6 cut(s) 9, 921, 945, 953, 1041, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 878
HpyF3I CTNAG 4 cut(s) 237, 282, 596, 667
HpySE526I ACGT 2 cut(s) 15, 723
Hsp92II CATG 9 cut(s) 9, 24, 65, 691, 701, 760, 885, 945, 1041
HspAI GCGC 1 cut(s) 169
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 3 cut(s) 226, 277, 1126
LguI GCTCTTC 2 cut(s) 140, 830
LmnI GCTCC 1 cut(s) 778
LweI GCATC 3 cut(s) 940, 1036, 1060
MaeII ACGT 2 cut(s) 15, 723
MaeIII GTNAC 3 cut(s) 304, 911, 1007
MalI GATC 3 cut(s) 228, 279, 1128
MboI GATC 3 cut(s) 226, 277, 1126
MboII GAAGA 5 cut(s) 157, 817, 843, 1121, 1132
MfeI CAATTG 1 cut(s) 1144
MflI RGATCY 3 cut(s) 226, 277, 1126
MhlI GDGCHC 2 cut(s) 541, 646
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 3 cut(s) 28, 92, 627
MmeI TCCRAC 1 cut(s) 1134
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 1 cut(s) 11
MreI CGCCGGCG 1 cut(s) 531
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 628, 847
MscI TGGCCA 1 cut(s) 438
MseI TTAA 3 cut(s) 635, 738, 1118
MslI CAYNNNNRTG 4 cut(s) 86, 702, 940, 1036
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 3 cut(s) 224, 336, 532
MspR9I CCNGG 3 cut(s) 563, 572, 786
MunI CAATTG 1 cut(s) 1144
MvaI CCWGG 3 cut(s) 563, 572, 786
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 166, 878
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 1 cut(s) 20
NdeI CATATG 2 cut(s) 163, 856
NdeII GATC 3 cut(s) 226, 277, 1126
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 9 cut(s) 9, 24, 65, 691, 701, 760, 885, 945, 1041
NlaIV GGNNCC 5 cut(s) 75, 279, 334, 493, 645
NmuCI GTSAC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 11
NspI RCATGY 4 cut(s) 9, 885, 945, 1041
PaeI GCATGC 1 cut(s) 9
PagI TCATGA 2 cut(s) 687, 697
PciSI GCTCTTC 2 cut(s) 140, 830
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 2 cut(s) 628, 847
PfeI GAWTC 3 cut(s) 110, 523, 996
PleI GAGTC 3 cut(s) 28, 92, 626
PpsI GAGTC 3 cut(s) 28, 92, 626
Psp6I CCWGG 3 cut(s) 561, 570, 784
PspGI CCWGG 3 cut(s) 561, 570, 784
PspN4I GGNNCC 5 cut(s) 75, 279, 334, 493, 645
PspPI GGNCC 1 cut(s) 675
PsuI RGATCY 3 cut(s) 226, 277, 1126
RsaI GTAC 5 cut(s) 363, 398, 410, 476, 560
RsaNI GTAC 5 cut(s) 362, 397, 409, 475, 559
RseI CAYNNNNRTG 4 cut(s) 86, 702, 940, 1036
SapI GCTCTTC 2 cut(s) 140, 830
SaqAI TTAA 3 cut(s) 635, 738, 1118
Sau3AI GATC 3 cut(s) 226, 277, 1126
Sau96I GGNCC 1 cut(s) 675
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 28, 92, 627
ScrFI CCNGG 3 cut(s) 563, 572, 786
SduI GDGCHC 2 cut(s) 541, 646
SfaNI GCATC 3 cut(s) 940, 1036, 1060
SfcI CTRYAG 2 cut(s) 1015, 1083
SgrAI CRCCGGYG 1 cut(s) 531
SmiMI CAYNNNNRTG 4 cut(s) 86, 702, 940, 1036
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
SphI GCATGC 1 cut(s) 9
SsiI CCGC 1 cut(s) 183
StyD4I CCNGG 3 cut(s) 561, 570, 784
StyI CCWWGG 2 cut(s) 20, 69
TaaI ACNGT 2 cut(s) 550, 611
TaiI ACGT 2 cut(s) 18, 726
TaqI TCGA 2 cut(s) 134, 999
TatI WGTACW 3 cut(s) 361, 396, 474
TfiI GAWTC 3 cut(s) 110, 523, 996
Tru1I TTAA 3 cut(s) 635, 738, 1118
Tru9I TTAA 3 cut(s) 635, 738, 1118
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 304
Tsp45I GTSAC 1 cut(s) 304
TspDTI ATGAA 3 cut(s) 676, 704, 843
TspRI CASTG 1 cut(s) 313
XagI CCTNNNNNAGG 1 cut(s) 670
XapI RAATTY 7 cut(s) 456, 589, 603, 627, 682, 734, 795
XceI RCATGY 4 cut(s) 9, 885, 945, 1041
XmnI GAANNNNTTC 2 cut(s) 628, 847
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.