Rh2BG542400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004903
Physical Location & Seq
Forward (+)
75055937 .. 75064159
8223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG542400.1

Sequence Viewer

Length: 1254 bp
ATGGCATACATATACATTCCATGGCTCTTGTTCTCTCAAATCCTCCTACAAGTAGCAAAACATGAGGCCAAGGTTCCGGCTCTCATTGTCTTCGGAGACTCCACCGTGGATTCCGGAAACAATAACAGGATTAAAACACTTTTGAAGAGCAACTTCAAGCCCTATGGCCGCGACTTTGCTGGGGGTCAACCCACAGGAAGGTTCTCCAATGGTCGTGTTCCTCCGGATTTCATTTCTGAGGCTTTTGGACTGAAACCATTCGTTCCGGCTTACTTGGATCCCAACTATGGGATTTCGGATTTTGTTACTGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGACAATGCAACTTCTGATGTACTGAATGTGATCCCTCTATGGAAAGAAGTGGAGTACTACAAGGAGTACCAGGCCAAATTGAGAGCCCATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAAGCTTTGTATGTGATAAGCTTGGGAACCAATGATTTCCTCGAGAACTACTACTTGCTACCCAGCCGGCAAGTCCAATATACAGTCCAGCAGTACCAGGACTTCCTTGTTGGACTAGCCGAAAACTTCATGAGGGAGCTTTACAGTCTTGGAGCCAGGAAGATTTCCCTAACTGGGCTCCCTCCCATGGGGTGCTTGCCCTTAGAGAGAGCCATAAATTTCATGGGTCACCATGATTGTGTGGAGGAGTACAACAATGTGGCCGTGGAGTTTAATGTGAAGTTGAACAACATGGTGGCAAAGCTTAACCAAGTGCTTCCAGGCTATAGAGTTCTGTTTACAGAGAAGGTCTATCAGTTTGTCTATCAAATGATCAGAACACCTTCCCAATTTGGGTTTGATGTTGCTGAAGTGGCATGTTGTTCTACTGGGAGATTCGAGATGAGTTACCTCTGTAGCGAACCAAATCCATATACATGCAAAGATGCAAATAAGTATATGTTCTGGGATGCCTTTCATCCTACAGAAAAAACAAATCATCCGATATTTATACATGTGCGTGGCTTCATCACCGCTCTTACTAAAAGAGAGTCTTCGTCAGAGTACGTCTCACGCGTTAAGGTTGAGTTTCACAAGAAAGACGACATCGCTGCTAAAGTCATCGCCGGAGCCTTGAAACCCGATTTGGGAGCGGATCGATTGGGAGTCGAGGGCGGCGTTGTCGGGTTCTATTCCAGCGCGTTAATATGGAAGAAGTTGATGAGGTTGTCGACGATTGGAAGTCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

417

Amino Acids

47.07

Weight (kDa)

8.29

Isoelectric Point (pI)

24.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 338 5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 1040, 1157
AccI GTMKAC 1 cut(s) 1235
AccII CGCG 3 cut(s) 171, 1080, 1205
AccIII TCCGGA 2 cut(s) 113, 223
AciI CCGC 4 cut(s) 169, 1038, 1157, 1179
AclWI GGATC 4 cut(s) 272, 285, 367, 1167
AcoI YGGCCR 3 cut(s) 166, 436, 726
AcsI RAATTY 2 cut(s) 456, 682
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 6 cut(s) 363, 398, 410, 560, 716, 1070
AfiI CCNNNNNNNGG 9 cut(s) 198, 287, 288, 445, 639, 652, 653, 858, 1151
AflIII ACRYGT 2 cut(s) 1018, 1078
AgsI TTSAA 4 cut(s) 145, 157, 751, 1141
AjnI CCWGG 4 cut(s) 411, 561, 620, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 1133, 1165
AluBI AGCT 4 cut(s) 470, 486, 604, 769
AluI AGCT 4 cut(s) 470, 486, 604, 769
Alw26I GTCTC 2 cut(s) 90, 1078
AlwI GGATC 4 cut(s) 272, 285, 367, 1167
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 2 cut(s) 113, 223
AoxI GGCC 5 cut(s) 66, 166, 414, 436, 726
ApeKI GCWGC 1 cut(s) 1115
ApoI RAATTY 2 cut(s) 456, 682
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 2 cut(s) 257, 847
AspLEI GCGC 1 cut(s) 1205
AsuHPI GGTGA 2 cut(s) 686, 1027
AvaI CYCGRG 1 cut(s) 506
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 430, 645
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 2 cut(s) 82, 1050
BbvI GCAGC 1 cut(s) 1102
BccI CCATC 1 cut(s) 447
BceAI ACGGC 1 cut(s) 713
BcgI CGANNNNNNTGC 2 cut(s) 1097, 1131
BciT130I CCWGG 4 cut(s) 413, 563, 622, 786
BclI TGATCA 1 cut(s) 837
BcoDI GTCTC 2 cut(s) 90, 1078
BfaI CTAG 2 cut(s) 581, 1252
BfmI CTRYAG 3 cut(s) 790, 919, 987
BisI GCNGC 3 cut(s) 169, 1116, 1180
BlsI GCNGC 3 cut(s) 170, 1117, 1181
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 4 cut(s) 413, 563, 622, 786
BmeT110I CYCGRG 1 cut(s) 506
BmiI GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 1135
BmrFI CCNGG 4 cut(s) 413, 563, 622, 786
BmrI ACTGGG 2 cut(s) 648, 903
BmsI GCATC 2 cut(s) 940, 964
BmuI ACTGGG 2 cut(s) 648, 903
BpiI GAAGAC 2 cut(s) 82, 1050
BplI GAGNNNNNCTC 2 cut(s) 1058, 1090
Bsa29I ATCGAT 1 cut(s) 1162
BsaJI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BsaWI WCCGGW 2 cut(s) 113, 223
Bsc4I CCNNNNNNNGG 9 cut(s) 198, 287, 288, 445, 639, 652, 653, 858, 1151
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 4 cut(s) 313, 340, 643, 898
BseAI TCCGGA 2 cut(s) 113, 223
BseBI CCWGG 4 cut(s) 413, 563, 622, 786
BseCI ATCGAT 1 cut(s) 1162
BseDI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BseGI GGATG 3 cut(s) 979, 982, 1003
BseLI CCNNNNNNNGG 9 cut(s) 198, 287, 288, 445, 639, 652, 653, 858, 1151
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 4 cut(s) 313, 340, 643, 898
BseRI GAGGAG 1 cut(s) 725
BseXI GCAGC 1 cut(s) 1102
BseYI CCCAGC 2 cut(s) 179, 527
Bsh1236I CGCG 3 cut(s) 171, 1080, 1205
BshFI GGCC 5 cut(s) 68, 168, 416, 438, 728
BshVI ATCGAT 1 cut(s) 1162
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 6 cut(s) 77, 114, 224, 266, 532, 1131
BslI CCNNNNNNNGG 9 cut(s) 198, 287, 288, 445, 639, 652, 653, 858, 1151
BsmAI GTCTC 2 cut(s) 90, 1078
BsmBI CGTCTC 1 cut(s) 1078
BsnI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 430, 645
Bsp13I TCCGGA 2 cut(s) 113, 223
Bsp143I GATC 4 cut(s) 277, 372, 837, 1159
Bsp19I CCATGG 2 cut(s) 20, 651
BspACI CCGC 4 cut(s) 169, 1038, 1157, 1179
BspANI GGCC 5 cut(s) 68, 168, 416, 438, 728
BspCNI CTCAG 1 cut(s) 229
BspDI ATCGAT 1 cut(s) 1162
BspEI TCCGGA 2 cut(s) 113, 223
BspFNI CGCG 3 cut(s) 171, 1080, 1205
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 1135
BspPI GGATC 4 cut(s) 272, 285, 367, 1167
BspQI GCTCTTC 1 cut(s) 140
BsrBI CCGCTC 2 cut(s) 1040, 1157
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 4 cut(s) 313, 340, 643, 898
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BssMI GATC 4 cut(s) 277, 372, 837, 1159
BssT1I CCWWGG 3 cut(s) 20, 69, 651
Bst2UI CCWGG 4 cut(s) 413, 563, 622, 786
Bst4CI ACNGT 3 cut(s) 106, 550, 611
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 533, 662
BstDEI CTNAG 2 cut(s) 237, 667
BstDSI CCRYGG 4 cut(s) 20, 105, 651, 729
BstEII GGTNACC 1 cut(s) 692
BstF5I GGATG 3 cut(s) 979, 982, 1003
BstFNI CGCG 3 cut(s) 171, 1080, 1205
BstHHI GCGC 1 cut(s) 1205
BstKTI GATC 4 cut(s) 280, 375, 840, 1162
BstMAI GTCTC 2 cut(s) 90, 1078
BstMBI GATC 4 cut(s) 277, 372, 837, 1159
BstMWI GCNNNNNNNGC 1 cut(s) 878
BstNI CCWGG 4 cut(s) 413, 563, 622, 786
BstNSI RCATGY 3 cut(s) 885, 945, 1022
BstPI GGTNACC 1 cut(s) 692
BstSCI CCNGG 4 cut(s) 411, 561, 620, 784
BstSFI CTRYAG 3 cut(s) 790, 919, 987
BstUI CGCG 3 cut(s) 171, 1080, 1205
BstV1I GCAGC 1 cut(s) 1102
BstV2I GAAGAC 2 cut(s) 82, 1050
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
Bsu15I ATCGAT 1 cut(s) 1162
BsuRI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsuTUI ATCGAT 1 cut(s) 1162
BtgI CCRYGG 4 cut(s) 20, 105, 651, 729
BtgZI GCGATG 2 cut(s) 1096, 1111
BtsCI GGATG 3 cut(s) 979, 982, 1003
Cac8I GCNNGC 2 cut(s) 533, 662
CciI TCATGA 1 cut(s) 594
CfoI GCGC 1 cut(s) 1205
Cfr10I RCCGGY 1 cut(s) 531
ClaI ATCGAT 1 cut(s) 1162
Csp6I GTAC 6 cut(s) 362, 397, 409, 559, 715, 1069
CviQI GTAC 6 cut(s) 362, 397, 409, 559, 715, 1069
DdeI CTNAG 2 cut(s) 237, 667
DpnI GATC 4 cut(s) 279, 374, 839, 1161
DpnII GATC 4 cut(s) 277, 372, 837, 1159
EaeI YGGCCR 3 cut(s) 166, 436, 726
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 20, 69, 651
Eco24I GRGCYC 2 cut(s) 430, 645
Eco57I CTGAAG 1 cut(s) 894
Eco88I CYCGRG 1 cut(s) 506
Eco91I GGTNACC 1 cut(s) 692
EcoO65I GGTNACC 1 cut(s) 692
EcoRII CCWGG 4 cut(s) 411, 561, 620, 784
EcoT14I CCWWGG 3 cut(s) 20, 69, 651
EcoT38I GRGCYC 2 cut(s) 430, 645
ErhI CCWWGG 3 cut(s) 20, 69, 651
Esp3I CGTCTC 1 cut(s) 1078
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 1042, 1074
FbaI TGATCA 1 cut(s) 837
FblI GTMKAC 1 cut(s) 1235
Fnu4HI GCNGC 3 cut(s) 169, 1116, 1180
FokI GGATG 3 cut(s) 969, 986, 990
FriOI GRGCYC 2 cut(s) 430, 645
Fsp4HI GCNGC 3 cut(s) 169, 1116, 1180
FspBI CTAG 2 cut(s) 581, 1252
GlaI GCGC 1 cut(s) 1204
GluI GCNGC 3 cut(s) 169, 1116, 1180
GsaI CCCAGC 2 cut(s) 183, 531
HaeIII GGCC 5 cut(s) 68, 168, 416, 438, 728
HapII CCGG 6 cut(s) 77, 114, 224, 266, 532, 1131
HhaI GCGC 1 cut(s) 1205
Hin6I GCGC 1 cut(s) 1203
HinP1I GCGC 1 cut(s) 1203
HincII GTYRAC 2 cut(s) 188, 1236
HindII GTYRAC 2 cut(s) 188, 1236
HindIII AAGCTT 3 cut(s) 468, 484, 767
HinfI GANTC 5 cut(s) 98, 110, 900, 1055, 1170
HpaII CCGG 6 cut(s) 77, 114, 224, 266, 532, 1131
HphI GGTGA 2 cut(s) 686, 1027
Hpy166II GTNNAC 3 cut(s) 188, 804, 1236
Hpy188I TCNGA 7 cut(s) 95, 238, 298, 358, 842, 1008, 1066
Hpy188III TCNNGA 5 cut(s) 114, 224, 508, 595, 904
Hpy8I GTNNAC 3 cut(s) 188, 804, 1236
Hpy99I CGWCG 1 cut(s) 1240
HpyAV CCTTC 3 cut(s) 192, 805, 858
HpyCH4III ACNGT 3 cut(s) 106, 550, 611
HpyCH4IV ACGT 1 cut(s) 1071
HpyCH4V TGCA 3 cut(s) 350, 945, 953
HpyF10VI GCNNNNNNNGC 1 cut(s) 878
HpyF3I CTNAG 2 cut(s) 237, 667
HpySE526I ACGT 1 cut(s) 1071
HspAI GCGC 1 cut(s) 1203
Kpn2I TCCGGA 2 cut(s) 113, 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 4 cut(s) 277, 372, 837, 1159
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 5 cut(s) 601, 617, 648, 1133, 1154
Lsp1109I GCAGC 1 cut(s) 1102
LweI GCATC 2 cut(s) 940, 964
MaeI CTAG 2 cut(s) 581, 1252
MaeII ACGT 1 cut(s) 1071
MaeIII GTNAC 3 cut(s) 304, 692, 911
MalI GATC 4 cut(s) 279, 374, 839, 1161
MbiI CCGCTC 2 cut(s) 1040, 1157
MboI GATC 4 cut(s) 277, 372, 837, 1159
MboII GAAGA 5 cut(s) 82, 157, 637, 1050, 1228
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 430, 645
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 4 cut(s) 419, 456, 682, 854
MluI ACGCGT 1 cut(s) 1078
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 3 cut(s) 92, 1064, 1179
MmeI TCCRAC 1 cut(s) 556
Mox20I TGGCCA 1 cut(s) 438
MroI TCCGGA 2 cut(s) 113, 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 257, 847
MscI TGGCCA 1 cut(s) 438
MseI TTAA 5 cut(s) 132, 738, 771, 1083, 1208
MslI CAYNNNNRTG 3 cut(s) 702, 940, 1023
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 6 cut(s) 77, 114, 224, 266, 532, 1131
MspR9I CCNGG 4 cut(s) 413, 563, 622, 786
MvaI CCWGG 4 cut(s) 413, 563, 622, 786
MvnI CGCG 3 cut(s) 171, 1080, 1205
MwoI GCNNNNNNNGC 1 cut(s) 878
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 2 cut(s) 20, 651
NdeII GATC 4 cut(s) 277, 372, 837, 1159
NgoMIV GCCGGC 1 cut(s) 531
NlaIV GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 1135
NmuCI GTSAC 1 cut(s) 692
NspI RCATGY 3 cut(s) 885, 945, 1022
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 594
PciI ACATGT 1 cut(s) 1018
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 2 cut(s) 1077, 1179
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 2 cut(s) 257, 847
PfeI GAWTC 2 cut(s) 110, 900
PkrI GCNGC 3 cut(s) 170, 1117, 1181
PleI GAGTC 3 cut(s) 92, 1063, 1178
PpsI GAGTC 3 cut(s) 92, 1063, 1178
PscI ACATGT 1 cut(s) 1018
Psp6I CCWGG 4 cut(s) 411, 561, 620, 784
PspEI GGTNACC 1 cut(s) 692
PspFI CCCAGC 2 cut(s) 179, 527
PspGI CCWGG 4 cut(s) 411, 561, 620, 784
PspN4I GGNNCC 6 cut(s) 75, 279, 493, 619, 644, 1135
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 6 cut(s) 363, 398, 410, 560, 716, 1070
RsaNI GTAC 6 cut(s) 362, 397, 409, 559, 715, 1069
RseI CAYNNNNRTG 3 cut(s) 702, 940, 1023
SalI GTCGAC 1 cut(s) 1234
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 5 cut(s) 132, 738, 771, 1083, 1208
SatI GCNGC 3 cut(s) 169, 1116, 1180
Sau3AI GATC 4 cut(s) 277, 372, 837, 1159
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 3 cut(s) 92, 1064, 1179
ScrFI CCNGG 4 cut(s) 413, 563, 622, 786
SduI GDGCHC 2 cut(s) 430, 645
SfaNI GCATC 2 cut(s) 940, 964
SfcI CTRYAG 3 cut(s) 790, 919, 987
Sfr274I CTCGAG 1 cut(s) 506
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 3 cut(s) 702, 940, 1023
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 4 cut(s) 419, 456, 682, 854
SsiI CCGC 4 cut(s) 169, 1038, 1157, 1179
SspMI CTAG 2 cut(s) 581, 1252
StyD4I CCNGG 4 cut(s) 411, 561, 620, 784
StyI CCWWGG 3 cut(s) 20, 69, 651
TaaI ACNGT 3 cut(s) 106, 550, 611
TaiI ACGT 1 cut(s) 1074
TaqI TCGA 5 cut(s) 507, 903, 1162, 1173, 1235
TasI AATT 4 cut(s) 419, 456, 682, 854
TatI WGTACW 3 cut(s) 361, 396, 714
TauI GCSGC 2 cut(s) 171, 1182
TfiI GAWTC 2 cut(s) 110, 900
Tru1I TTAA 5 cut(s) 132, 738, 771, 1083, 1208
Tru9I TTAA 5 cut(s) 132, 738, 771, 1083, 1208
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 1 cut(s) 1115
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 5 cut(s) 220, 583, 676, 971, 1021
XapI RAATTY 2 cut(s) 456, 682
XceI RCATGY 3 cut(s) 885, 945, 1022
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XhoI CTCGAG 1 cut(s) 506
XmiI GTMKAC 1 cut(s) 1235
XmnI GAANNNNTTC 2 cut(s) 257, 847
XspI CTAG 2 cut(s) 581, 1252
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.