RchiOBHm_Chr2g0157741

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004903
Physical Location & Seq
Forward (+)
74053900 .. 74059138
5239 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ52646

Sequence Viewer

Length: 1053 bp
ATGGCATACATATACATTCCATGGCTCTTGTTCTCTCAAATCCTCCTACAAGTAGCAAAACATGAGGCCAAGGTTCCGGCTCTCATTGTCTTCGGAGACTCCACCGTGGATTCCGGAAACAATAACCGGATTAAAACACTTTTGAAGAGCAACTTCAAGCCCTATGGCCGCGACTTTGCTGGGGGTCAACCCACAGGAAGGTTCTCCAATGGTCGTGTTCCTCCGGATTTCATTTCTGAGGCTTTTGGACTGAAACCATTCGTTCCGGCTTACTTGGATCCCGACTATGGGATTTCGGATTTTGTTACTGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGACAATGCAACTTCTGATGTACTAAATGTGATCCCTCTATGGAAAGAAGTGGAGTACTACAAGGAGTACCAGGCCAAATTGAGAGCCCATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAAGCTTTGTATGTGATAAGCTTGGGAACCAATGATTTCCTCGAGAACTACTACTTGCTACCCCGCCGGCAAGTCCAATATACAGTCCAGCAGTACCAGGACTTCCTTGTTGGACTAGCCGAAAACTTCATGAGGGAGCTTTACAGTCTTGGAGCCAGGAAGATTTCCCTAACTGGGCTCCCTCCCATGGGGTGCTTGCCCTTAGAGAGAGCCATAAATTTCATTGGTCACCATGATTGTGTGGAGGAGTACAACAATGTGGCCGTGGAGTTTAATGTGAAGTTGAACAACATGGTGGCAAAGCTTAACCAAGAGCTTCCAGGCTATAGAGTTCTGTTTACAGAGAAGGTCTATCAGTTTGTCTATCAAATAATCAGAACACCTTCCCTATTTGGGTTTGATGTTGTTGAAGTGGCATGTTGTTCTACTGGGAGATTCGAGATGAGTTACCTCTGTAGCGAACCAAATCCATATACATGCAAAGATGCAAATAAGTATATGTTCTGGGATGCCTTCCATCCTACAGAAAAAACAAATCAGTTAGTCTCCAACTATTTAATTCCAGATCTTCTACAATTCTACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.96

Weight (kDa)

5.84

Isoelectric Point (pI)

24.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 340 5.8e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 171
AccIII TCCGGA 2 cut(s) 113, 223
AciI CCGC 2 cut(s) 169, 529
AclWI GGATC 3 cut(s) 272, 285, 367
AcoI YGGCCR 3 cut(s) 166, 436, 726
AcsI RAATTY 2 cut(s) 456, 682
AfaI GTAC 5 cut(s) 363, 398, 410, 560, 716
AfiI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
AgsI TTSAA 4 cut(s) 145, 157, 751, 875
AjnI CCWGG 4 cut(s) 411, 561, 620, 784
AluBI AGCT 5 cut(s) 470, 486, 604, 769, 781
AluI AGCT 5 cut(s) 470, 486, 604, 769, 781
Alw26I GTCTC 2 cut(s) 90, 1015
AlwI GGATC 3 cut(s) 272, 285, 367
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 2 cut(s) 113, 223
AoxI GGCC 5 cut(s) 66, 166, 414, 436, 726
ApoI RAATTY 2 cut(s) 456, 682
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 2 cut(s) 257, 847
AsuHPI GGTGA 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 506
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 430, 645
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 1 cut(s) 82
BccI CCATC 2 cut(s) 447, 990
BceAI ACGGC 1 cut(s) 713
BciT130I CCWGG 4 cut(s) 413, 563, 622, 786
BcoDI GTCTC 2 cut(s) 90, 1015
BfaI CTAG 1 cut(s) 581
BfmI CTRYAG 3 cut(s) 790, 919, 987
BglII AGATCT 1 cut(s) 1030
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 4 cut(s) 413, 563, 622, 786
BmeT110I CYCGRG 1 cut(s) 506
BmiI GGNNCC 5 cut(s) 75, 279, 493, 619, 644
BmrFI CCNGG 4 cut(s) 413, 563, 622, 786
BmrI ACTGGG 2 cut(s) 648, 903
BmsI GCATC 2 cut(s) 940, 964
BmuI ACTGGG 2 cut(s) 648, 903
BpiI GAAGAC 1 cut(s) 82
BsaJI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BsaWI WCCGGW 3 cut(s) 113, 126, 223
Bsc4I CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 4 cut(s) 313, 340, 643, 898
BseAI TCCGGA 2 cut(s) 113, 223
BseBI CCWGG 4 cut(s) 413, 563, 622, 786
BseDI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BseGI GGATG 2 cut(s) 979, 982
BseLI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 4 cut(s) 313, 340, 643, 898
BseRI GAGGAG 1 cut(s) 725
BseYI CCCAGC 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 6 cut(s) 77, 114, 127, 224, 266, 532
BslI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
BsmAI GTCTC 2 cut(s) 90, 1015
BsnI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 430, 645
Bsp13I TCCGGA 2 cut(s) 113, 223
Bsp143I GATC 3 cut(s) 277, 372, 1030
Bsp19I CCATGG 2 cut(s) 20, 651
BspACI CCGC 2 cut(s) 169, 529
BspANI GGCC 5 cut(s) 68, 168, 416, 438, 728
BspCNI CTCAG 1 cut(s) 229
BspEI TCCGGA 2 cut(s) 113, 223
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 5 cut(s) 75, 279, 493, 619, 644
BspPI GGATC 3 cut(s) 272, 285, 367
BspQI GCTCTTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 4 cut(s) 313, 340, 643, 898
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BssMI GATC 3 cut(s) 277, 372, 1030
BssT1I CCWWGG 3 cut(s) 20, 69, 651
Bst2UI CCWGG 4 cut(s) 413, 563, 622, 786
Bst4CI ACNGT 3 cut(s) 106, 550, 611
Bst6I CTCTTC 1 cut(s) 140
BstC8I GCNNGC 2 cut(s) 533, 662
BstDEI CTNAG 2 cut(s) 237, 667
BstDSI CCRYGG 4 cut(s) 20, 105, 651, 729
BstEII GGTNACC 1 cut(s) 692
BstF5I GGATG 2 cut(s) 979, 982
BstFNI CGCG 1 cut(s) 171
BstKTI GATC 3 cut(s) 280, 375, 1033
BstMAI GTCTC 2 cut(s) 90, 1015
BstMBI GATC 3 cut(s) 277, 372, 1030
BstNI CCWGG 4 cut(s) 413, 563, 622, 786
BstNSI RCATGY 2 cut(s) 885, 945
BstPI GGTNACC 1 cut(s) 692
BstSCI CCNGG 4 cut(s) 411, 561, 620, 784
BstSFI CTRYAG 3 cut(s) 790, 919, 987
BstUI CGCG 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 277, 1030
BstYI RGATCY 2 cut(s) 277, 1030
BsuRI GGCC 5 cut(s) 68, 168, 416, 438, 728
BtgI CCRYGG 4 cut(s) 20, 105, 651, 729
BtsCI GGATG 2 cut(s) 979, 982
Cac8I GCNNGC 2 cut(s) 533, 662
CciI TCATGA 1 cut(s) 594
Cfr10I RCCGGY 1 cut(s) 531
Csp6I GTAC 5 cut(s) 362, 397, 409, 559, 715
CviAII CATG 9 cut(s) 21, 62, 431, 595, 652, 698, 757, 882, 942
CviQI GTAC 5 cut(s) 362, 397, 409, 559, 715
DdeI CTNAG 2 cut(s) 237, 667
DpnI GATC 3 cut(s) 279, 374, 1032
DpnII GATC 3 cut(s) 277, 372, 1030
EaeI YGGCCR 3 cut(s) 166, 436, 726
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 3 cut(s) 20, 69, 651
Eco24I GRGCYC 2 cut(s) 430, 645
Eco88I CYCGRG 1 cut(s) 506
Eco91I GGTNACC 1 cut(s) 692
EcoO65I GGTNACC 1 cut(s) 692
EcoRII CCWGG 4 cut(s) 411, 561, 620, 784
EcoT14I CCWWGG 3 cut(s) 20, 69, 651
EcoT38I GRGCYC 2 cut(s) 430, 645
ErhI CCWWGG 3 cut(s) 20, 69, 651
FaeI CATG 9 cut(s) 24, 65, 434, 598, 655, 701, 760, 885, 945
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 9 cut(s) 20, 61, 430, 594, 651, 697, 756, 881, 941
FauI CCCGC 1 cut(s) 536
Fnu4HI GCNGC 1 cut(s) 169
FokI GGATG 2 cut(s) 969, 986
FriOI GRGCYC 2 cut(s) 430, 645
Fsp4HI GCNGC 1 cut(s) 169
FspBI CTAG 1 cut(s) 581
GluI GCNGC 1 cut(s) 169
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 5 cut(s) 68, 168, 416, 438, 728
HapII CCGG 6 cut(s) 77, 114, 127, 224, 266, 532
Hin1II CATG 9 cut(s) 24, 65, 434, 598, 655, 701, 760, 885, 945
HincII GTYRAC 1 cut(s) 188
HindII GTYRAC 1 cut(s) 188
HindIII AAGCTT 3 cut(s) 468, 484, 767
HinfI GANTC 3 cut(s) 98, 110, 900
HpaII CCGG 6 cut(s) 77, 114, 127, 224, 266, 532
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 2 cut(s) 188, 804
Hpy188I TCNGA 5 cut(s) 95, 238, 298, 358, 842
Hpy188III TCNNGA 7 cut(s) 114, 224, 281, 508, 595, 904, 1028
Hpy8I GTNNAC 2 cut(s) 188, 804
HpyAV CCTTC 4 cut(s) 192, 805, 858, 988
HpyCH4III ACNGT 3 cut(s) 106, 550, 611
HpyCH4V TGCA 3 cut(s) 350, 945, 953
HpyF3I CTNAG 2 cut(s) 237, 667
Hsp92II CATG 9 cut(s) 24, 65, 434, 598, 655, 701, 760, 885, 945
Kpn2I TCCGGA 2 cut(s) 113, 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 3 cut(s) 277, 372, 1030
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 3 cut(s) 601, 617, 648
LweI GCATC 2 cut(s) 940, 964
MaeI CTAG 1 cut(s) 581
MaeIII GTNAC 3 cut(s) 304, 692, 911
MalI GATC 3 cut(s) 279, 374, 1032
MboI GATC 3 cut(s) 277, 372, 1030
MboII GAAGA 4 cut(s) 82, 157, 637, 1025
MfeI CAATTG 1 cut(s) 1048
MflI RGATCY 2 cut(s) 277, 1030
MhlI GDGCHC 2 cut(s) 430, 645
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 6 cut(s) 419, 456, 682, 1023, 1040, 1048
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 556, 1038
Mox20I TGGCCA 1 cut(s) 438
MroI TCCGGA 2 cut(s) 113, 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 257, 847
MscI TGGCCA 1 cut(s) 438
MseI TTAA 4 cut(s) 132, 738, 771, 1022
MslI CAYNNNNRTG 2 cut(s) 702, 940
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 6 cut(s) 77, 114, 127, 224, 266, 532
MspR9I CCNGG 4 cut(s) 413, 563, 622, 786
MunI CAATTG 1 cut(s) 1048
MvaI CCWGG 4 cut(s) 413, 563, 622, 786
MvnI CGCG 1 cut(s) 171
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 2 cut(s) 20, 651
NdeII GATC 3 cut(s) 277, 372, 1030
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 9 cut(s) 24, 65, 434, 598, 655, 701, 760, 885, 945
NlaIV GGNNCC 5 cut(s) 75, 279, 493, 619, 644
NmuCI GTSAC 1 cut(s) 692
NspI RCATGY 2 cut(s) 885, 945
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 594
PciSI GCTCTTC 1 cut(s) 140
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 2 cut(s) 257, 847
PfeI GAWTC 2 cut(s) 110, 900
PkrI GCNGC 1 cut(s) 170
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 4 cut(s) 411, 561, 620, 784
PspEI GGTNACC 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 4 cut(s) 411, 561, 620, 784
PspN4I GGNNCC 5 cut(s) 75, 279, 493, 619, 644
PsuI RGATCY 2 cut(s) 277, 1030
RsaI GTAC 5 cut(s) 363, 398, 410, 560, 716
RsaNI GTAC 5 cut(s) 362, 397, 409, 559, 715
RseI CAYNNNNRTG 2 cut(s) 702, 940
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 132, 738, 771, 1022
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 3 cut(s) 277, 372, 1030
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 4 cut(s) 413, 563, 622, 786
SduI GDGCHC 2 cut(s) 430, 645
SfaNI GCATC 2 cut(s) 940, 964
SfcI CTRYAG 3 cut(s) 790, 919, 987
Sfr274I CTCGAG 1 cut(s) 506
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 2 cut(s) 702, 940
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 6 cut(s) 419, 456, 682, 1023, 1040, 1048
SsiI CCGC 2 cut(s) 169, 529
SspMI CTAG 1 cut(s) 581
StyD4I CCNGG 4 cut(s) 411, 561, 620, 784
StyI CCWWGG 3 cut(s) 20, 69, 651
TaaI ACNGT 3 cut(s) 106, 550, 611
TaqI TCGA 2 cut(s) 507, 903
TasI AATT 6 cut(s) 419, 456, 682, 1023, 1040, 1048
TatI WGTACW 3 cut(s) 361, 396, 714
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 2 cut(s) 110, 900
Tru1I TTAA 4 cut(s) 132, 738, 771, 1022
Tru9I TTAA 4 cut(s) 132, 738, 771, 1022
TseFI GTSAC 1 cut(s) 692
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 3 cut(s) 220, 583, 676
XapI RAATTY 2 cut(s) 456, 682
XceI RCATGY 2 cut(s) 885, 945
XhoI CTCGAG 1 cut(s) 506
XmnI GAANNNNTTC 2 cut(s) 257, 847
XspI CTAG 1 cut(s) 581
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.