FvH4_6g41873

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004903
Physical Location & Seq
Forward (+)
32800799 .. 32804457
3659 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41873.t1

Sequence Viewer

Length: 1053 bp
ATGGCATACATTTACATTCCATGGCTCTTGTTCTCTCAAATCCTCCTGCAAGTAGCAAAACATGAGGCTAAGGTTCCGGCCATTATTGTCTTCGGAGACTCGACCGTGGATTCTGGAAACAACAACCGGATTTCGACACTTTTGAAGAGCAACTTCAGGCCATATGGGCGCGACTTTGCTGGGGGTCAACCCACAGGCAGGTTCTGCAATGGTCGTGTTCCTCCGGATTTCATTTCTGATGCTTTTGGACTTAAACCATTCGTCCCGGCTTACTTGGATCCCAACTATGGGATTTCGGATTTTGTCACTGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGACAATGCAACCTCTGATGTACTAAATGTGATACCTCTATGGAAAGAAGTGGAGTACTACAAGGAATACCAGGCCAAATTGAGAGCCCATGTTGGCCATGACAAGGCAACAGAAATTTTGGGAGAAGCTTTGTATGTTATAAGCTTGGGAACCAATGATTTCCTGGAGAACTACTACTTGCTACCCCGCCGGCAAGTCCAATATACAGTCCAGCAGTACCAGGACTTCCTCGTCGGACTAGCCGAAAACTTCATGAGGGAGCTTTACAGTCTTGGAGTCACGAAGATTTCCCTATCTGGGCTCCCTCCCATGGGGTGCTTGCCCTTAGAGAGAGCCACAAATTTCATAGGTCACCATGATTGTGTGGAGGAGTACAACAATGTGGCTGTGGAGTTTAATGTGAAGTTGAACAACATGGTGGCAAAGCTTAACACAGAGCTTCCGGGATATAGAGTTCTGTTTACGGAGAAGGTTTATCAGTTCGTCTATCAAATGATCAGAACACCTTCCCAGTTCGGGTTTGAGGTTACTGAAGTGGCATGTTGTTCTACTGGAACATTCGAGATGAGTTACCTCTGTAGTGAATCAAATCCATATACATGCAAAGACGCAAACAAGTATATGTTCTGGGATGCCTTCCATCCTACAGAAAAAACAAATAAGATAGTCTCCGACTTTTTAATTCCAGATCTTCTGGCATTCTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

351

Amino Acids

39.64

Weight (kDa)

5.23

Isoelectric Point (pI)

23.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 339 1e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AasI GACNNNNNNGTC 1 cut(s) 572
Acc36I ACCTGC 1 cut(s) 189
AccII CGCG 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 1 cut(s) 529
AclWI GGATC 2 cut(s) 272, 285
AcoI YGGCCR 2 cut(s) 78, 436
AcsI RAATTY 2 cut(s) 456, 682
AcuI CTGAAG 2 cut(s) 139, 894
AfaI GTAC 4 cut(s) 363, 398, 560, 716
AfiI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
AgsI TTSAA 3 cut(s) 145, 751, 1048
AjnI CCWGG 3 cut(s) 411, 504, 561
AloI GAACNNNNNNTCC 2 cut(s) 780, 812
AluBI AGCT 5 cut(s) 470, 486, 604, 769, 781
AluI AGCT 5 cut(s) 470, 486, 604, 769, 781
Alw26I GTCTC 2 cut(s) 90, 1015
AlwI GGATC 2 cut(s) 272, 285
AlwNI CAGNNNCTG 1 cut(s) 204
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 4 cut(s) 78, 158, 414, 436
ApoI RAATTY 2 cut(s) 456, 682
Asp700I GAANNNNTTC 1 cut(s) 847
AspLEI GCGC 1 cut(s) 171
AsuC2I CCSGG 2 cut(s) 266, 786
AsuHPI GGTGA 1 cut(s) 686
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 430, 645
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 1 cut(s) 82
BccI CCATC 1 cut(s) 990
BciT130I CCWGG 3 cut(s) 413, 506, 563
BclI TGATCA 1 cut(s) 837
BcnI CCSGG 2 cut(s) 266, 786
BcoDI GTCTC 2 cut(s) 90, 1015
BfaI CTAG 1 cut(s) 581
BfmI CTRYAG 2 cut(s) 919, 987
BfuAI ACCTGC 1 cut(s) 189
BglI GCCNNNNNGGC 1 cut(s) 166
BglII AGATCT 1 cut(s) 1030
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 5 cut(s) 266, 413, 506, 563, 786
BmiI GGNNCC 4 cut(s) 75, 279, 493, 644
BmrFI CCNGG 5 cut(s) 266, 413, 506, 563, 786
BmrI ACTGGG 1 cut(s) 847
BmsI GCATC 2 cut(s) 229, 964
BmuI ACTGGG 1 cut(s) 847
BpiI GAAGAC 1 cut(s) 82
BpmI CTGGAG 1 cut(s) 527
Bpu10I CCTNAGC 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 266, 786
BsaJI CCNNGG 3 cut(s) 20, 105, 651
BsaWI WCCGGW 2 cut(s) 126, 223
Bsc4I CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 4 cut(s) 313, 340, 853, 898
Bse3DI GCAATG 1 cut(s) 214
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 3 cut(s) 413, 506, 563
BseDI CCNNGG 3 cut(s) 20, 105, 651
BseGI GGATG 2 cut(s) 979, 982
BseLI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
BseMI GCAATG 1 cut(s) 214
BseNI ACTGG 4 cut(s) 313, 340, 853, 898
BseRI GAGGAG 1 cut(s) 725
BseYI CCCAGC 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 171
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 80, 160, 416, 438
BsiEI CGRYCG 1 cut(s) 105
BsiSI CCGG 6 cut(s) 77, 127, 224, 266, 532, 785
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 8 cut(s) 198, 287, 288, 445, 639, 652, 653, 858
BsmAI GTCTC 2 cut(s) 90, 1015
BsmFI GGGAC 1 cut(s) 248
BsmI GAATGC 1 cut(s) 1040
BsnI GGCC 4 cut(s) 80, 160, 416, 438
Bsp1286I GDGCHC 2 cut(s) 430, 645
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 3 cut(s) 277, 837, 1030
Bsp19I CCATGG 2 cut(s) 20, 651
BspACI CCGC 1 cut(s) 529
BspANI GGCC 4 cut(s) 80, 160, 416, 438
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 4 cut(s) 75, 279, 493, 644
BspMI ACCTGC 1 cut(s) 189
BspPI GGATC 2 cut(s) 272, 285
BspQI GCTCTTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 214
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 4 cut(s) 313, 340, 853, 898
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 3 cut(s) 20, 105, 651
BssMI GATC 3 cut(s) 277, 837, 1030
BssT1I CCWWGG 2 cut(s) 20, 651
Bst2UI CCWGG 3 cut(s) 413, 506, 563
Bst4CI ACNGT 3 cut(s) 106, 550, 611
Bst6I CTCTTC 1 cut(s) 140
BstAPI GCANNNNNTGC 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 533, 662
BstDEI CTNAG 2 cut(s) 69, 667
BstDSI CCRYGG 3 cut(s) 20, 105, 651
BstEII GGTNACC 1 cut(s) 692
BstF5I GGATG 2 cut(s) 979, 982
BstFNI CGCG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 3 cut(s) 280, 840, 1033
BstMAI GTCTC 2 cut(s) 90, 1015
BstMBI GATC 3 cut(s) 277, 837, 1030
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 2 cut(s) 166, 204
BstNI CCWGG 3 cut(s) 413, 506, 563
BstNSI RCATGY 2 cut(s) 885, 945
BstPI GGTNACC 1 cut(s) 692
BstSCI CCNGG 5 cut(s) 264, 411, 504, 561, 784
BstSFI CTRYAG 2 cut(s) 919, 987
BstUI CGCG 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 2 cut(s) 277, 1030
BstYI RGATCY 2 cut(s) 277, 1030
BsuRI GGCC 4 cut(s) 80, 160, 416, 438
BtgI CCRYGG 3 cut(s) 20, 105, 651
BtsCI GGATG 2 cut(s) 979, 982
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 189
Cac8I GCNNGC 2 cut(s) 533, 662
CaiI CAGNNNCTG 1 cut(s) 204
CciI TCATGA 1 cut(s) 594
CfoI GCGC 1 cut(s) 171
Cfr10I RCCGGY 1 cut(s) 531
CseI GACGC 1 cut(s) 959
Csp6I GTAC 4 cut(s) 362, 397, 559, 715
CviQI GTAC 4 cut(s) 362, 397, 559, 715
DdeI CTNAG 2 cut(s) 69, 667
DpnI GATC 3 cut(s) 279, 839, 1032
DpnII GATC 3 cut(s) 277, 837, 1030
DrdI GACNNNNNNGTC 1 cut(s) 572
DseDI GACNNNNNNGTC 1 cut(s) 572
EaeI YGGCCR 2 cut(s) 78, 436
Eam1104I CTCTTC 1 cut(s) 140
EarI CTCTTC 1 cut(s) 140
Eco130I CCWWGG 2 cut(s) 20, 651
Eco24I GRGCYC 2 cut(s) 430, 645
Eco57I CTGAAG 2 cut(s) 139, 894
Eco91I GGTNACC 1 cut(s) 692
EcoO65I GGTNACC 1 cut(s) 692
EcoRII CCWGG 3 cut(s) 411, 504, 561
EcoT14I CCWWGG 2 cut(s) 20, 651
EcoT38I GRGCYC 2 cut(s) 430, 645
ErhI CCWWGG 2 cut(s) 20, 651
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 248
FauI CCCGC 1 cut(s) 536
FauNDI CATATG 1 cut(s) 163
FbaI TGATCA 1 cut(s) 837
FokI GGATG 2 cut(s) 969, 986
FriOI GRGCYC 2 cut(s) 430, 645
FspBI CTAG 1 cut(s) 581
GlaI GCGC 1 cut(s) 170
GsaI CCCAGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 527
HaeIII GGCC 4 cut(s) 80, 160, 416, 438
HapII CCGG 6 cut(s) 77, 127, 224, 266, 532, 785
HgaI GACGC 1 cut(s) 959
HhaI GCGC 1 cut(s) 171
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 188
HindII GTYRAC 1 cut(s) 188
HindIII AAGCTT 3 cut(s) 468, 484, 767
HinfI GANTC 4 cut(s) 98, 110, 618, 926
HpaII CCGG 6 cut(s) 77, 127, 224, 266, 532, 785
HphI GGTGA 1 cut(s) 686
Hpy166II GTNNAC 2 cut(s) 188, 804
Hpy188I TCNGA 7 cut(s) 95, 238, 298, 358, 578, 842, 1015
Hpy188III TCNNGA 6 cut(s) 114, 224, 595, 622, 904, 1028
Hpy8I GTNNAC 2 cut(s) 188, 804
Hpy99I CGWCG 1 cut(s) 578
HpyAV CCTTC 3 cut(s) 805, 858, 988
HpyCH4III ACNGT 3 cut(s) 106, 550, 611
HpyCH4V TGCA 4 cut(s) 49, 207, 350, 945
HpyF10VI GCNNNNNNNGC 2 cut(s) 166, 204
HpyF3I CTNAG 2 cut(s) 69, 667
HspAI GCGC 1 cut(s) 169
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Ksp22I TGATCA 1 cut(s) 837
Kzo9I GATC 3 cut(s) 277, 837, 1030
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 2 cut(s) 601, 648
LweI GCATC 2 cut(s) 229, 964
MaeI CTAG 1 cut(s) 581
MaeIII GTNAC 5 cut(s) 304, 619, 692, 868, 911
MalI GATC 3 cut(s) 279, 839, 1032
MboI GATC 3 cut(s) 277, 837, 1030
MboII GAAGA 5 cut(s) 82, 157, 637, 1025, 1036
MfeI CAATTG 1 cut(s) 1048
MflI RGATCY 2 cut(s) 277, 1030
MhlI GDGCHC 2 cut(s) 430, 645
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 5 cut(s) 419, 456, 682, 1023, 1048
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 2 cut(s) 92, 627
MmeI TCCRAC 2 cut(s) 556, 1038
Mox20I TGGCCA 1 cut(s) 438
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 847
MscI TGGCCA 1 cut(s) 438
MseI TTAA 4 cut(s) 252, 738, 771, 1022
MslI CAYNNNNRTG 2 cut(s) 702, 940
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 6 cut(s) 77, 127, 224, 266, 532, 785
MspR9I CCNGG 5 cut(s) 266, 413, 506, 563, 786
MunI CAATTG 1 cut(s) 1048
Mva1269I GAATGC 1 cut(s) 1040
MvaI CCWGG 3 cut(s) 413, 506, 563
MvnI CGCG 1 cut(s) 171
MwoI GCNNNNNNNGC 2 cut(s) 166, 204
NaeI GCCGGC 1 cut(s) 533
NciI CCSGG 2 cut(s) 266, 786
NcoI CCATGG 2 cut(s) 20, 651
NdeI CATATG 1 cut(s) 163
NdeII GATC 3 cut(s) 277, 837, 1030
NgoMIV GCCGGC 1 cut(s) 531
NlaIV GGNNCC 4 cut(s) 75, 279, 493, 644
NmuCI GTSAC 3 cut(s) 304, 619, 692
NspI RCATGY 2 cut(s) 885, 945
PagI TCATGA 1 cut(s) 594
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PctI GAATGC 1 cut(s) 1040
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 847
PfeI GAWTC 2 cut(s) 110, 926
PfoI TCCNGGA 2 cut(s) 504, 784
PleI GAGTC 2 cut(s) 92, 626
PpsI GAGTC 2 cut(s) 92, 626
PsiI TTATAA 1 cut(s) 482
Psp6I CCWGG 3 cut(s) 411, 504, 561
PspEI GGTNACC 1 cut(s) 692
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 3 cut(s) 411, 504, 561
PspN4I GGNNCC 4 cut(s) 75, 279, 493, 644
PstNI CAGNNNCTG 1 cut(s) 204
PsuI RGATCY 2 cut(s) 277, 1030
RsaI GTAC 4 cut(s) 363, 398, 560, 716
RsaNI GTAC 4 cut(s) 362, 397, 559, 715
RseI CAYNNNNRTG 2 cut(s) 702, 940
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 252, 738, 771, 1022
Sau3AI GATC 3 cut(s) 277, 837, 1030
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 2 cut(s) 92, 627
ScrFI CCNGG 5 cut(s) 266, 413, 506, 563, 786
SduI GDGCHC 2 cut(s) 430, 645
SfaNI GCATC 2 cut(s) 229, 964
SfcI CTRYAG 2 cut(s) 919, 987
SmiMI CAYNNNNRTG 2 cut(s) 702, 940
Sse9I AATT 5 cut(s) 419, 456, 682, 1023, 1048
SsiI CCGC 1 cut(s) 529
SspMI CTAG 1 cut(s) 581
StyD4I CCNGG 5 cut(s) 264, 411, 504, 561, 784
StyI CCWWGG 2 cut(s) 20, 651
TaaI ACNGT 3 cut(s) 106, 550, 611
TaqI TCGA 3 cut(s) 101, 134, 903
TasI AATT 5 cut(s) 419, 456, 682, 1023, 1048
TatI WGTACW 3 cut(s) 361, 396, 714
TfiI GAWTC 2 cut(s) 110, 926
Tru1I TTAA 4 cut(s) 252, 738, 771, 1022
Tru9I TTAA 4 cut(s) 252, 738, 771, 1022
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 3 cut(s) 304, 619, 692
Tsp45I GTSAC 3 cut(s) 304, 619, 692
TspDTI ATGAA 3 cut(s) 220, 583, 676
TspGWI ACGGA 1 cut(s) 821
TspRI CASTG 1 cut(s) 313
XapI RAATTY 2 cut(s) 456, 682
XceI RCATGY 2 cut(s) 885, 945
XcmI CCANNNNNNNNNTGG 1 cut(s) 502
XmnI GAANNNNTTC 1 cut(s) 847
XspI CTAG 1 cut(s) 581
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.