Rh2CG512400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004903
Physical Location & Seq
Forward (+)
68635221 .. 68655694
20474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG512400.1

Sequence Viewer

Length: 864 bp
ATGGCATACATATACATTCCATGGCTCTTGTTCTCACAAATCCTCCTACAAGTAGCAAAACATGAGGCCAAGGTTCCGGCTCTCATTGTCTTCGGAGACTCCACCGTGGATTCTGGAAACAATAACCGGATTAAAACACTTTTGAAGAGCAACTTCAAGCCCTATGGCCGCGACTTTGCTGGGGGTCACCCCACAGGAAGGTTCTCCAATGGTCGTGTTCCTCCGGATTTCATTTCTGAGGCTTTTGGACTGAAACCATTCGTTCCGGCTTACTTGGATCCCAACTATGGGATTTCGGATTTTGTTACTGGTGTTTGCTTTGCTTCTGCTGGAACTGGCTATGACAATGCAACTTCTGATGTACTAAATGTGATCCCTCTATGGAAAGAAGTGGAGTACTACAAGGAGTACCAGGCCAAATTGAGAGCCCATGTTGGCCATCACAAGGCAAGAGAAATTTTGACAGAAGCTTTGTATGTGATAAGCTTGGGAACCAATGATTTCCTCGAGAACTACTACTTGCTACCCCGCCGGCAAGTCCAATATACAGTCCAGCAGTACCAGGACTTCCTTGTTGGACTAGCCGAAAACTTCATGAGGGAGCTTTACAGTCTTGGAGCCAGGAAGATTTCCCTAACTGGGCTCCCTCCCATGGGGTGCTTGCCCTTAGAGAGAGCCATAAATTTCATGGGTCACCATGATTGTGTGGAGGAGTACAACAATGTGGCCGTGGAGTTTAATGTGAAGTTGAACAGCATGGTGGCAAAGCTTAACCAAGAGCTTCCAGGCTATAGAGTTCTGTTTACAGAGAAGAGCAAAAAACGTATCTCCAAAGACAAACTTCAAAGGGCGACAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

287

Amino Acids

32.53

Weight (kDa)

8.26

Isoelectric Point (pI)

21.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 29 - 267 1.1e-25 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 2 cut(s) 169, 529
AclWI GGATC 3 cut(s) 272, 285, 367
AcoI YGGCCR 3 cut(s) 166, 436, 726
AcsI RAATTY 2 cut(s) 456, 682
AfaI GTAC 5 cut(s) 363, 398, 410, 560, 716
AfiI CCNNNNNNNGG 7 cut(s) 198, 287, 288, 445, 639, 652, 653
AgsI TTSAA 4 cut(s) 145, 157, 751, 845
AjnI CCWGG 4 cut(s) 411, 561, 620, 784
AluBI AGCT 5 cut(s) 470, 486, 604, 769, 781
AluI AGCT 5 cut(s) 470, 486, 604, 769, 781
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 3 cut(s) 272, 285, 367
Ama87I CYCGRG 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 5 cut(s) 66, 166, 414, 436, 726
ApoI RAATTY 2 cut(s) 456, 682
ArsI GACNNNNNNTTYG 2 cut(s) 454, 486
Asp700I GAANNNNTTC 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 179, 686
AvaI CYCGRG 1 cut(s) 506
BalI TGGCCA 1 cut(s) 438
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 430, 645
BarI GAAGNNNNNNTAC 2 cut(s) 551, 583
BbsI GAAGAC 1 cut(s) 82
BccI CCATC 1 cut(s) 447
BceAI ACGGC 1 cut(s) 713
BciT130I CCWGG 4 cut(s) 413, 563, 622, 786
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 581
BfmI CTRYAG 1 cut(s) 790
BisI GCNGC 1 cut(s) 169
BlsI GCNGC 1 cut(s) 170
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 4 cut(s) 413, 563, 622, 786
BmeT110I CYCGRG 1 cut(s) 506
BmiI GGNNCC 5 cut(s) 75, 279, 493, 619, 644
BmrFI CCNGG 4 cut(s) 413, 563, 622, 786
BmrI ACTGGG 1 cut(s) 648
BmuI ACTGGG 1 cut(s) 648
BpiI GAAGAC 1 cut(s) 82
BsaJI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BsaWI WCCGGW 2 cut(s) 126, 223
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 7 cut(s) 198, 287, 288, 445, 639, 652, 653
Bse118I RCCGGY 1 cut(s) 531
Bse1I ACTGG 3 cut(s) 313, 340, 643
BseAI TCCGGA 1 cut(s) 223
BseBI CCWGG 4 cut(s) 413, 563, 622, 786
BseDI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BseLI CCNNNNNNNGG 7 cut(s) 198, 287, 288, 445, 639, 652, 653
BseMII CTCAG 1 cut(s) 228
BseNI ACTGG 3 cut(s) 313, 340, 643
BseRI GAGGAG 1 cut(s) 725
BseYI CCCAGC 1 cut(s) 179
Bsh1236I CGCG 1 cut(s) 171
BshFI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsiHKCI CYCGRG 1 cut(s) 506
BsiSI CCGG 5 cut(s) 77, 127, 224, 266, 532
BslI CCNNNNNNNGG 7 cut(s) 198, 287, 288, 445, 639, 652, 653
BsmAI GTCTC 1 cut(s) 90
BsnI GGCC 5 cut(s) 68, 168, 416, 438, 728
BsoBI CYCGRG 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 430, 645
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 2 cut(s) 277, 372
Bsp19I CCATGG 2 cut(s) 20, 651
BspACI CCGC 2 cut(s) 169, 529
BspANI GGCC 5 cut(s) 68, 168, 416, 438, 728
BspCNI CTCAG 1 cut(s) 229
BspEI TCCGGA 1 cut(s) 223
BspFNI CGCG 1 cut(s) 171
BspHI TCATGA 1 cut(s) 594
BspLI GGNNCC 5 cut(s) 75, 279, 493, 619, 644
BspPI GGATC 3 cut(s) 272, 285, 367
BspQI GCTCTTC 2 cut(s) 140, 806
BsrFI RCCGGY 1 cut(s) 531
BsrI ACTGG 3 cut(s) 313, 340, 643
BssAI RCCGGY 1 cut(s) 531
BssECI CCNNGG 5 cut(s) 20, 69, 105, 651, 729
BssMI GATC 2 cut(s) 277, 372
BssT1I CCWWGG 3 cut(s) 20, 69, 651
Bst2UI CCWGG 4 cut(s) 413, 563, 622, 786
Bst4CI ACNGT 3 cut(s) 106, 550, 611
Bst6I CTCTTC 2 cut(s) 140, 806
BstC8I GCNNGC 2 cut(s) 533, 662
BstDEI CTNAG 2 cut(s) 237, 667
BstDSI CCRYGG 4 cut(s) 20, 105, 651, 729
BstEII GGTNACC 2 cut(s) 185, 692
BstFNI CGCG 1 cut(s) 171
BstKTI GATC 2 cut(s) 280, 375
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 2 cut(s) 277, 372
BstNI CCWGG 4 cut(s) 413, 563, 622, 786
BstPI GGTNACC 2 cut(s) 185, 692
BstSCI CCNGG 4 cut(s) 411, 561, 620, 784
BstSFI CTRYAG 1 cut(s) 790
BstUI CGCG 1 cut(s) 171
BstV2I GAAGAC 1 cut(s) 82
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 5 cut(s) 68, 168, 416, 438, 728
BtgI CCRYGG 4 cut(s) 20, 105, 651, 729
Cac8I GCNNGC 2 cut(s) 533, 662
CciI TCATGA 1 cut(s) 594
Cfr10I RCCGGY 1 cut(s) 531
Csp6I GTAC 5 cut(s) 362, 397, 409, 559, 715
CviAII CATG 8 cut(s) 21, 62, 431, 595, 652, 688, 698, 757
CviQI GTAC 5 cut(s) 362, 397, 409, 559, 715
DdeI CTNAG 2 cut(s) 237, 667
DpnI GATC 2 cut(s) 279, 374
DpnII GATC 2 cut(s) 277, 372
EaeI YGGCCR 3 cut(s) 166, 436, 726
Eam1104I CTCTTC 2 cut(s) 140, 806
EarI CTCTTC 2 cut(s) 140, 806
Eco130I CCWWGG 3 cut(s) 20, 69, 651
Eco24I GRGCYC 2 cut(s) 430, 645
Eco88I CYCGRG 1 cut(s) 506
Eco91I GGTNACC 2 cut(s) 185, 692
EcoO65I GGTNACC 2 cut(s) 185, 692
EcoRII CCWGG 4 cut(s) 411, 561, 620, 784
EcoT14I CCWWGG 3 cut(s) 20, 69, 651
EcoT38I GRGCYC 2 cut(s) 430, 645
ErhI CCWWGG 3 cut(s) 20, 69, 651
FaeI CATG 8 cut(s) 24, 65, 434, 598, 655, 691, 701, 760
FalI AAGNNNNNCTT 4 cut(s) 137, 169, 825, 857
FatI CATG 8 cut(s) 20, 61, 430, 594, 651, 687, 697, 756
FauI CCCGC 1 cut(s) 536
Fnu4HI GCNGC 1 cut(s) 169
FriOI GRGCYC 2 cut(s) 430, 645
Fsp4HI GCNGC 1 cut(s) 169
FspBI CTAG 1 cut(s) 581
GluI GCNGC 1 cut(s) 169
GsaI CCCAGC 1 cut(s) 183
HaeIII GGCC 5 cut(s) 68, 168, 416, 438, 728
HapII CCGG 5 cut(s) 77, 127, 224, 266, 532
Hin1II CATG 8 cut(s) 24, 65, 434, 598, 655, 691, 701, 760
HindIII AAGCTT 3 cut(s) 468, 484, 767
HinfI GANTC 2 cut(s) 98, 110
HpaII CCGG 5 cut(s) 77, 127, 224, 266, 532
HphI GGTGA 2 cut(s) 179, 686
Hpy166II GTNNAC 1 cut(s) 804
Hpy188I TCNGA 4 cut(s) 95, 238, 298, 358
Hpy188III TCNNGA 4 cut(s) 114, 224, 508, 595
Hpy8I GTNNAC 1 cut(s) 804
HpyAV CCTTC 1 cut(s) 192
HpyCH4III ACNGT 3 cut(s) 106, 550, 611
HpyCH4IV ACGT 1 cut(s) 823
HpyCH4V TGCA 1 cut(s) 350
HpyF3I CTNAG 2 cut(s) 237, 667
HpySE526I ACGT 1 cut(s) 823
Hsp92II CATG 8 cut(s) 24, 65, 434, 598, 655, 691, 701, 760
Kpn2I TCCGGA 1 cut(s) 223
KroI GCCGGC 1 cut(s) 531
KroNI GCCGGC 1 cut(s) 533
Kzo9I GATC 2 cut(s) 277, 372
LguI GCTCTTC 2 cut(s) 140, 806
LmnI GCTCC 3 cut(s) 601, 617, 648
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 823
MaeIII GTNAC 3 cut(s) 185, 304, 692
MalI GATC 2 cut(s) 279, 374
MboI GATC 2 cut(s) 277, 372
MboII GAAGA 4 cut(s) 82, 157, 637, 823
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 430, 645
MlsI TGGCCA 1 cut(s) 438
MluCI AATT 3 cut(s) 419, 456, 682
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 9 cut(s) 53, 58, 231, 232, 387, 515, 591, 657, 703
Mox20I TGGCCA 1 cut(s) 438
MroI TCCGGA 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 531
MroXI GAANNNNTTC 1 cut(s) 257
MscI TGGCCA 1 cut(s) 438
MseI TTAA 4 cut(s) 132, 738, 771, 862
MslI CAYNNNNRTG 1 cut(s) 702
Msp20I TGGCCA 1 cut(s) 438
MspI CCGG 5 cut(s) 77, 127, 224, 266, 532
MspR9I CCNGG 4 cut(s) 413, 563, 622, 786
MvaI CCWGG 4 cut(s) 413, 563, 622, 786
MvnI CGCG 1 cut(s) 171
NaeI GCCGGC 1 cut(s) 533
NcoI CCATGG 2 cut(s) 20, 651
NdeII GATC 2 cut(s) 277, 372
NgoMIV GCCGGC 1 cut(s) 531
NlaIII CATG 8 cut(s) 24, 65, 434, 598, 655, 691, 701, 760
NlaIV GGNNCC 5 cut(s) 75, 279, 493, 619, 644
NmuCI GTSAC 2 cut(s) 185, 692
PaeR7I CTCGAG 1 cut(s) 506
PagI TCATGA 1 cut(s) 594
PciSI GCTCTTC 2 cut(s) 140, 806
PdiI GCCGGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 110
PkrI GCNGC 1 cut(s) 170
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Psp6I CCWGG 4 cut(s) 411, 561, 620, 784
PspEI GGTNACC 2 cut(s) 185, 692
PspFI CCCAGC 1 cut(s) 179
PspGI CCWGG 4 cut(s) 411, 561, 620, 784
PspN4I GGNNCC 5 cut(s) 75, 279, 493, 619, 644
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 5 cut(s) 363, 398, 410, 560, 716
RsaNI GTAC 5 cut(s) 362, 397, 409, 559, 715
RseI CAYNNNNRTG 1 cut(s) 702
SapI GCTCTTC 2 cut(s) 140, 806
SaqAI TTAA 4 cut(s) 132, 738, 771, 862
SatI GCNGC 1 cut(s) 169
Sau3AI GATC 2 cut(s) 277, 372
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 4 cut(s) 413, 563, 622, 786
SduI GDGCHC 2 cut(s) 430, 645
SetI ASST 8 cut(s) 75, 203, 472, 488, 606, 771, 783, 826
SfcI CTRYAG 1 cut(s) 790
Sfr274I CTCGAG 1 cut(s) 506
SlaI CTCGAG 1 cut(s) 506
SmiMI CAYNNNNRTG 1 cut(s) 702
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 3 cut(s) 419, 456, 682
SsiI CCGC 2 cut(s) 169, 529
SspMI CTAG 1 cut(s) 581
StyD4I CCNGG 4 cut(s) 411, 561, 620, 784
StyI CCWWGG 3 cut(s) 20, 69, 651
TaaI ACNGT 3 cut(s) 106, 550, 611
TaiI ACGT 1 cut(s) 826
TaqI TCGA 1 cut(s) 507
TasI AATT 3 cut(s) 419, 456, 682
TatI WGTACW 3 cut(s) 361, 396, 714
TauI GCSGC 1 cut(s) 171
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 4 cut(s) 132, 738, 771, 862
Tru9I TTAA 4 cut(s) 132, 738, 771, 862
TseFI GTSAC 2 cut(s) 185, 692
Tsp45I GTSAC 2 cut(s) 185, 692
TspDTI ATGAA 3 cut(s) 220, 583, 676
XapI RAATTY 2 cut(s) 456, 682
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XhoI CTCGAG 1 cut(s) 506
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 1 cut(s) 581
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.